Roland WOHLGEMUTH

Summary

Affiliation: Sigma-Aldrich
Location: Buchs, Schweiz
Publications:
1.
Swiss Industrial Biocatalysis Consortium (SIBC).
Wirz B, Kittelmann M, Meyer HP, Wohlgemuth R.
Chimia (Aarau). 2010;64(11):780-1.

2.
Biocatalysis--key to sustainable industrial chemistry.
Wohlgemuth R.
Curr Opin Biotechnol. 2010 Dec;21(6):713-24. Epub 2010 Oct 26. Review.

3.
Asymmetric biocatalysis with microbial enzymes and cells.
Wohlgemuth R.
Curr Opin Microbiol. 2010 Jun;13(3):283-92. Epub 2010 Apr 29. Review.

4.
Characterization of a whole-cell catalyst co-expressing glycerol dehydrogenase and glucose dehydrogenase and its application in the synthesis of L-glyceraldehyde.
Richter N, Neumann M, Liese A, Wohlgemuth R, Weckbecker A, Eggert T, Hummel W.
Biotechnol Bioeng. 2010 Jul 1;106(4):541-52.

5.
Guidelines for reporting of biocatalytic reactions.
Gardossi L, Poulsen PB, Ballesteros A, Hult K, Svedas VK, Vasić-Racki D, Carrea G, Magnusson A, Schmid A, Wohlgemuth R, Halling PJ; European Federation of Biotechnology Section on Applied Biocatalysis.
Trends Biotechnol. 2010 Apr;28(4):171-80. Epub 2010 Feb 10.

6.
Tools and ingredients for the biocatalytic synthesis of metabolites.
Wohlgemuth R.
Biotechnol J. 2009 Sep;4(9):1253-65. Review.

7.
Characterisation of a recombinant NADP-dependent glycerol dehydrogenase from Gluconobacter oxydans and its application in the production of L-glyceraldehyde.
Richter N, Neumann M, Liese A, Wohlgemuth R, Eggert T, Hummel W.
Chembiochem. 2009 Jul 20;10(11):1888-96.

8.
Influence of pH on the expression of a recombinant epoxide hydrolase in Aspergillus niger.
Naundorf A, Melzer G, Archelas A, Furstoss R, Wohlgemuth R.
Biotechnol J. 2009 May;4(5):756-65.

9.
Preparative-scale separation by simulated moving bed chromatography of biocatalytically produced regioisomeric lactones.
Kaiser P, Ottolina G, Carrea G, Wohlgemuth R.
N Biotechnol. 2009 Apr;25(4):220-5. Epub 2009 Jan 22.

10.
The locks and keys to industrial biotechnology.
Wohlgemuth R.
N Biotechnol. 2009 Apr;25(4):204-13. Epub 2009 Jan 21. Review.

11.
Characterization of enzymatic D-xylulose 5-phosphate synthesis.
Shaeri J, Wright I, Rathbone EB, Wohlgemuth R, Woodley JM.
Biotechnol Bioeng. 2008 Nov 1;101(4):761-7.

12.
Preparative scale Baeyer-Villiger biooxidation at high concentration using recombinant Escherichia coli and in situ substrate feeding and product removal process.
Hilker I, Gutiérrez MC, Furstoss R, Ward J, Wohlgemuth R, Alphand V.
Nat Protoc. 2008;3(3):546-54. Epub 2008 Mar 6.

13.
Production of epoxide hydrolases in batch fermentations of Botryosphaeria rhodina.
Melzer G, Junne S, Wohlgemuth R, Hempel DC, Götz P.
J Ind Microbiol Biotechnol. 2008 Jun;35(6):485-93. Epub 2008 Jan 22.

14.
Ex vivo glycan engineering of CD44 programs human multipotent mesenchymal stromal cell trafficking to bone.
Sackstein R, Merzaban JS, Cain DW, Dagia NM, Spencer JA, Lin CP, Wohlgemuth R.
Nat Med. 2008 Feb;14(2):181-7. Epub 2008 Jan 13.

15.
Process analysis of macrotetrolide biosynthesis during fermentation by means of direct infusion LC-MS.
Jani P, Emmert J, Wohlgemuth R.
Biotechnol J. 2008 Feb;3(2):202-8.

16.
On the influence of oxygen and cell concentration in an SFPR whole cell biocatalytic Baeyer-Villiger oxidation process.
Hilker I, Baldwin C, Alphand V, Furstoss R, Woodley J, Wohlgemuth R.
Biotechnol Bioeng. 2006 Apr 20;93(6):1138-44.

17.
Microbial transformations 59: first kilogram scale asymmetric microbial Baeyer-Villiger oxidation with optimized productivity using a resin-based in situ SFPR strategy.
Hilker I, Wohlgemuth R, Alphand V, Furstoss R.
Biotechnol Bioeng. 2005 Dec 20;92(6):702-10.
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18.
Microbiological transformations 57. Facile and efficient resin-based in situ SFPR preparative-scale synthesis of an enantiopure "unexpected" lactone regioisomer via a Baeyer-Villiger oxidation process.
Hilker I, Gutiérrez MC, Alphand V, Wohlgemuth R, Furstoss R.
Org Lett. 2004 Jun 10;6(12):1955-8.

19.
Towards large-scale synthetic applications of Baeyer-Villiger monooxygenases.
Alphand V, Carrea G, Wohlgemuth R, Furstoss R, Woodley JM.
Trends Biotechnol. 2003 Jul;21(7):318-23. Review.

20.
Reactor operation and scale-up of whole cell Baeyer-Villiger catalyzed lactone synthesis.
Doig SD, Avenell PJ, Bird PA, Gallati P, Lander KS, Lye GJ, Wohlgemuth R, Woodley JM.
Biotechnol Prog. 2002 Sep-Oct;18(5):1039-46.

Publications

  1. ncbi Reactor operation and scale-up of whole cell Baeyer-Villiger catalyzed lactone synthesis
    Steven D Doig
    Department of Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, UK
    Biotechnol Prog 18:1039-46. 2002
  2. doi Asymmetric biocatalysis with microbial enzymes and cells
    Roland Wohlgemuth
    Research Specialties, Sigma Aldrich, Industriestrasse 25, CH 9470 Buchs, Switzerland
    Curr Opin Microbiol 13:283-92. 2010
  3. doi Characterization of a whole-cell catalyst co-expressing glycerol dehydrogenase and glucose dehydrogenase and its application in the synthesis of L-glyceraldehyde
    Nina Richter
    Evocatal GmbH, Merowingerplatz 1a, 40225 Dusseldorf, Germany
    Biotechnol Bioeng 106:541-52. 2010
  4. doi Guidelines for reporting of biocatalytic reactions
    Lucia Gardossi
    Dipartimento di Scienze Farmaceutiche, Universita di Trieste, Piazzale Europa 1, 34127 Trieste, Italy
    Trends Biotechnol 28:171-80. 2010
  5. doi Tools and ingredients for the biocatalytic synthesis of metabolites
    Roland Wohlgemuth
    Research Specialties, Sigma Aldrich, Buchs, Switzerland
    Biotechnol J 4:1253-65. 2009
  6. doi Characterisation of a recombinant NADP-dependent glycerol dehydrogenase from Gluconobacter oxydans and its application in the production of L-glyceraldehyde
    Nina Richter
    Evocatal GmbH, Merowingerplatz 1 A, 40225 Dusseldorf, Germany
    Chembiochem 10:1888-96. 2009
  7. doi Influence of pH on the expression of a recombinant epoxide hydrolase in Aspergillus niger
    Andreas Naundorf
    Research Specialties, Sigma Aldrich, Buchs, Switzerland
    Biotechnol J 4:756-65. 2009
  8. doi Preparative-scale separation by simulated moving bed chromatography of biocatalytically produced regioisomeric lactones
    Patrick Kaiser
    SAFC Process Research and Development, Industriestrasse 25, CH 9470 Buchs, Switzerland
    N Biotechnol 25:220-5. 2009
  9. doi The locks and keys to industrial biotechnology
    Roland Wohlgemuth
    Sigma Aldrich, Research Specialities, Industriestrasse 25, 9470 Buchs, Switzerland
    N Biotechnol 25:204-13. 2009
  10. doi Characterization of enzymatic D-xylulose 5-phosphate synthesis
    Jobin Shaeri
    Department of Biochemical Engineering, University College London, Torrington Place, London, UK
    Biotechnol Bioeng 101:761-7. 2008

Collaborators

Detail Information

Publications19

  1. ncbi Reactor operation and scale-up of whole cell Baeyer-Villiger catalyzed lactone synthesis
    Steven D Doig
    Department of Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, UK
    Biotechnol Prog 18:1039-46. 2002
    ....
  2. doi Asymmetric biocatalysis with microbial enzymes and cells
    Roland Wohlgemuth
    Research Specialties, Sigma Aldrich, Industriestrasse 25, CH 9470 Buchs, Switzerland
    Curr Opin Microbiol 13:283-92. 2010
    ..The production of a wide variety of industrial products by asymmetric biocatalysis has even become the preferred method of production...
  3. doi Characterization of a whole-cell catalyst co-expressing glycerol dehydrogenase and glucose dehydrogenase and its application in the synthesis of L-glyceraldehyde
    Nina Richter
    Evocatal GmbH, Merowingerplatz 1a, 40225 Dusseldorf, Germany
    Biotechnol Bioeng 106:541-52. 2010
    ..The results obtained making the described whole-cell catalyst an improved catalyst for a more efficient production of enantiopure L-glyceraldehyde...
  4. doi Guidelines for reporting of biocatalytic reactions
    Lucia Gardossi
    Dipartimento di Scienze Farmaceutiche, Universita di Trieste, Piazzale Europa 1, 34127 Trieste, Italy
    Trends Biotechnol 28:171-80. 2010
    ..We believe that adoption and use of these guidelines could greatly increase the value and impact of published work in biocatalysis, and hence promote the further growth of applications...
  5. doi Tools and ingredients for the biocatalytic synthesis of metabolites
    Roland Wohlgemuth
    Research Specialties, Sigma Aldrich, Buchs, Switzerland
    Biotechnol J 4:1253-65. 2009
    ..The use of stable-isotope-labeled metabolites can thereby simplify in vivo applications and metabolic flux analyses...
  6. doi Characterisation of a recombinant NADP-dependent glycerol dehydrogenase from Gluconobacter oxydans and its application in the production of L-glyceraldehyde
    Nina Richter
    Evocatal GmbH, Merowingerplatz 1 A, 40225 Dusseldorf, Germany
    Chembiochem 10:1888-96. 2009
    ..Since high enantioselectivities were observed for the reduction of glyceraldehyde, the kinetic resolution of glyceraldehyde was investigated and found to yield enantiopure L-glyceraldehyde on preparative scale...
  7. doi Influence of pH on the expression of a recombinant epoxide hydrolase in Aspergillus niger
    Andreas Naundorf
    Research Specialties, Sigma Aldrich, Buchs, Switzerland
    Biotechnol J 4:756-65. 2009
    ..The EH has been easily isolated from the biomass with high activity (1.67 U/mg lyophilisate) in a robust process...
  8. doi Preparative-scale separation by simulated moving bed chromatography of biocatalytically produced regioisomeric lactones
    Patrick Kaiser
    SAFC Process Research and Development, Industriestrasse 25, CH 9470 Buchs, Switzerland
    N Biotechnol 25:220-5. 2009
    ..Under optimised conditions, the productivity of the process was 0.026g/(g(stationary)(phase) day) of pure regioisomers and the solvent consumption was 0.363L/g(feed)...
  9. doi The locks and keys to industrial biotechnology
    Roland Wohlgemuth
    Sigma Aldrich, Research Specialities, Industriestrasse 25, 9470 Buchs, Switzerland
    N Biotechnol 25:204-13. 2009
    ..Biocatalyst and pathway discovery and development is the key towards new synthetic transformations in industrial biotechnology...
  10. doi Characterization of enzymatic D-xylulose 5-phosphate synthesis
    Jobin Shaeri
    Department of Biochemical Engineering, University College London, Torrington Place, London, UK
    Biotechnol Bioeng 101:761-7. 2008
    ..The data presented here allow a comparison of both routes for the synthesis, based on experimental information on reaction kinetics. Operational guidelines are proposed which should assist in the scale-up of such syntheses...
  11. doi Preparative scale Baeyer-Villiger biooxidation at high concentration using recombinant Escherichia coli and in situ substrate feeding and product removal process
    Iris Hilker
    Biosciences CNRS 3005, Aix Marseille Universite, Avenue Escadre Normandie Niemen, 13397 Marseille, France
    Nat Protoc 3:546-54. 2008
    ..The corresponding lactone was obtained in 75-80% yield. Time for cell growth and biotransformation is about 24 h each and oxygen supply can be improved by using a tailor-made bubble column...
  12. doi Production of epoxide hydrolases in batch fermentations of Botryosphaeria rhodina
    Guido Melzer
    Institute of Biochemical Engineering, Technische Universitat Braunschweig, Gaussstrasse 17, 38106 Braunschweig, Germany
    J Ind Microbiol Biotechnol 35:485-93. 2008
    ..4 times higher when using R-pNSO. A protein purification strategy leading to a 47-fold increase in specific activity (940 U/mgProtein) was developed as a first step to investigate molecular and structural characteristics of the EH...
  13. doi Ex vivo glycan engineering of CD44 programs human multipotent mesenchymal stromal cell trafficking to bone
    Robert Sackstein
    Department of Dermatology, Brigham and Women s Hospital and Harvard Skin Disease Research Center, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Med 14:181-7. 2008
    ..These findings establish that the HCELL glycoform of CD44 confers tropism to bone and unveil a readily translatable roadmap for programming cellular trafficking by chemical engineering of glycans on a distinct membrane glycoprotein...
  14. ncbi Process analysis of macrotetrolide biosynthesis during fermentation by means of direct infusion LC-MS
    Peter Jani
    Research Specialties, Sigma Aldrich, Buchs, Switzerland
    Biotechnol J 3:202-8. 2008
    ..In this work, a fast and versatile analytical LC-MS method has been set up, which has proven of much value for the in-process analysis of macrotetrolides during fermentation and which has allowed rapid large-scale bioprocess development...
  15. ncbi On the influence of oxygen and cell concentration in an SFPR whole cell biocatalytic Baeyer-Villiger oxidation process
    Iris Hilker
    Department of Biochemistry, Fluka Group, Industriestrasse 25, CH 9470 Buchs, Switzerland
    Biotechnol Bioeng 93:1138-44. 2006
    ..Both parameters were optimized with respect to practical considerations. The outcome of this study supports a schematic process model, allows estimation of optimum process conditions and exploration of its limits...
  16. ncbi Microbial transformations 59: first kilogram scale asymmetric microbial Baeyer-Villiger oxidation with optimized productivity using a resin-based in situ SFPR strategy
    Iris Hilker
    Groupe Biocatalyse et Chimie Fine, FRE CNRS 2712, Universite de la Mediterranee, Faculte des Sciences de Luminy, Case 901, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France
    Biotechnol Bioeng 92:702-10. 2005
    ..This novel resin-based in situ SFPR technology therefore clearly opens the way to further (industrial) upscaling of this highly valuable (asymmetric) reaction...
  17. ncbi Microbiological transformations 57. Facile and efficient resin-based in situ SFPR preparative-scale synthesis of an enantiopure "unexpected" lactone regioisomer via a Baeyer-Villiger oxidation process
    Iris Hilker
    Groupe Biocatalyse et Chimie Fine, UMR CNRS 6111, Universite de la Mediterranee, Faculte des Sciences de Luminy, Case 901, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France
    Org Lett 6:1955-8. 2004
    ..2 g L(-)(1) h(-)(1)). This illustrates the high "sustainable chemistry" advantages of such a process, simply conducted in aqueous medium, at room temperature and using atmospheric oxygen...
  18. ncbi Towards large-scale synthetic applications of Baeyer-Villiger monooxygenases
    Veronique Alphand
    Groupe Biocatalyse et Chimie Fine, CNRS, Case 901, Marseille 13288, France
    Trends Biotechnol 21:318-23. 2003
    ..Here, we review the state-of-the-art situation for the scale-up and exploitation of these enzymes...
  19. doi Biocatalysis--key to sustainable industrial chemistry
    Roland Wohlgemuth
    Sigma Aldrich, Industriestrasse 25, CH 9470 Buchs, Switzerland
    Curr Opin Biotechnol 21:713-24. 2010
    ..The understanding of the catalytic functions of known and new enzymes is key for the development of new sustainable chemical transformations...