Affiliation: The Scripps Research Institute
- Nucleotide dependent packing differences in helical crystals of the ABC transporter MsbAAndrew Ward
Department of Cell Biology, The Scripps Research Institute, 10550 N Torrey Pines Rd, CB227, La Jolla, CA 92037, USA
J Struct Biol 165:169-75. 2009..This suggests that while there are likely structural differences between the nucleotide transition states, membrane embedded MsbA remains in an outward facing conformation while nucleotide is bound...
- Windex: a toolset for indexing helicesAndrew Ward
Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
J Struct Biol 144:172-83. 2003..The new procedures have been tested using a number of helical specimens, including TMV, acto-myosin, decorated microtubules, and a variety of helical tubes of a bacterial membrane protein...
- The structures of MsbA: Insight into ABC transporter-mediated multidrug effluxChristopher L Reyes
Department of Molecular Biology, The Scripps Research Institute, 10550 N Torrey Pines Rd, CB105, La Jolla, CA 92137, USA
FEBS Lett 580:1042-8. 2006..In this review, we examine recent structural and functional analysis of the ABC transporter MsbA and implications on the mechanism of multidrug efflux...
- Flexibility in the ABC transporter MsbA: Alternating access with a twistAndrew Ward
Departments of Cell Biology and Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, CB 105, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 104:19005-10. 2007..Altogether, the conformational changes between these structures suggest that large ranges of motion may be required for substrate transport...
- Differential binding of neutralizing and non-neutralizing antibodies to native-like soluble HIV-1 Env trimers, uncleaved Env proteins, and monomeric subunitsAnila Yasmeen
Department of Microbiology and Immunology, Weill Cornell Medical College, Cornell University, New York, USA
Retrovirology 11:41. 2014..By introducing stabilizing mutations (SOSIP), we constructed soluble, cleaved Env trimers derived from the HIV-1 subtype A isolate BG505 that resemble native Env spikes on virions both structurally and antigenically...
- Stable 293 T and CHO cell lines expressing cleaved, stable HIV-1 envelope glycoprotein trimers for structural and vaccine studiesNancy P Y Chung
Department of Microbiology and Immunology, Weill Cornell Medical College, New York, NY, USA
Retrovirology 11:33. 2014..Making cell lines that produce BG505 SOSIP.664 trimers requires the co-expression of the Furin protease to ensure that the cleavage site between the gp120 and gp41 subunits is fully utilized...
- Broadly neutralizing antibody PGT121 allosterically modulates CD4 binding via recognition of the HIV-1 gp120 V3 base and multiple surrounding glycansJean Philippe Julien
Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA
PLoS Pathog 9:e1003342. 2013....
- Structure of P-glycoprotein reveals a molecular basis for poly-specific drug bindingStephen G Aller
Department of Molecular Biology, Scripps Research Institute, 10550 North Torrey Pines Road, CB105, La Jolla, CA 92037, USA
Science 323:1718-22. 2009..The inward-facing conformation represents an initial stage of the transport cycle that is competent for drug binding...
- A helical processing pipeline for EM structure determination of membrane proteinsLauren S Fisher
The National Resource for Automated Molecular Microscopy, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Methods 55:350-62. 2011..We describe here the integration of standard Fourier-Bessel helical analysis techniques into Appion, an integrated, database-driven pipeline...
- Designing facial amphiphiles for the stabilization of integral membrane proteinsQinghai Zhang
Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Angew Chem Int Ed Engl 46:7023-5. 2007