Lei Wang

Summary

Affiliation: The Salk Institute
Country: USA

Publications

  1. ncbi request reprint [Dynamic change of metabolism related protein in liver tissue of rats' model of hepatic fibrosis and regulatory effect of fuzheng huayu decoction on it]
    Ying Liu
    Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203
    Zhongguo Zhong Xi Yi Jie He Za Zhi 26:224-7. 2006
  2. pmc Release factor one is nonessential in Escherichia coli
    David B F Johnson
    The Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, 10010 N Torrey Pines Road, La Jolla, CA 92037, USA
    ACS Chem Biol 7:1337-44. 2012
  3. pmc Stereochemical basis for engineered pyrrolysyl-tRNA synthetase and the efficient in vivo incorporation of structurally divergent non-native amino acids
    Jeffrey K Takimoto
    The Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, United States
    ACS Chem Biol 6:733-43. 2011
  4. ncbi request reprint Evolving proteins in mammalian cells using somatic hypermutation
    Lei Wang
    The Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, 10010 N Torrey Pines Road, La Jolla, California 92037, USA
    Nat Protoc 1:1346-50. 2006
  5. pmc RF1 knockout allows ribosomal incorporation of unnatural amino acids at multiple sites
    David B F Johnson
    The Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, La Jolla, California, USA
    Nat Chem Biol 7:779-86. 2011
  6. doi request reprint Human dopamine β-hydroxylase promoter variant alters transcription in chromaffin cells, enzyme secretion, and blood pressure
    Yuqing Chen
    Department of Medicine, University of California at San Diego, La Jolla, USA
    Am J Hypertens 24:24-32. 2011
  7. doi request reprint Improving orthogonal tRNA-synthetase recognition for efficient unnatural amino acid incorporation and application in mammalian cells
    Jeffrey K Takimoto
    The Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, 10010 N Torrey Pines Road, La Jolla, CA 92037, USA
    Mol Biosyst 5:931-4. 2009
  8. ncbi request reprint A new strategy for the site-specific modification of proteins in vivo
    Zhiwen Zhang
    Department of Chemistry and Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Biochemistry 42:6735-46. 2003
  9. pmc Adding an unnatural covalent bond to proteins through proximity-enhanced bioreactivity
    Zheng Xiang
    The Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, La Jolla, California, USA
    Nat Methods 10:885-8. 2013
  10. ncbi request reprint Expanding the genetic code
    Lei Wang
    The Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Annu Rev Biophys Biomol Struct 35:225-49. 2006

Collaborators

Detail Information

Publications71

  1. ncbi request reprint [Dynamic change of metabolism related protein in liver tissue of rats' model of hepatic fibrosis and regulatory effect of fuzheng huayu decoction on it]
    Ying Liu
    Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203
    Zhongguo Zhong Xi Yi Jie He Za Zhi 26:224-7. 2006
    ..To investigate the effect of Fuzheng Huayu decoction (FHD) intervention on hepatic fibrosis...
  2. pmc Release factor one is nonessential in Escherichia coli
    David B F Johnson
    The Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, 10010 N Torrey Pines Road, La Jolla, CA 92037, USA
    ACS Chem Biol 7:1337-44. 2012
    ....
  3. pmc Stereochemical basis for engineered pyrrolysyl-tRNA synthetase and the efficient in vivo incorporation of structurally divergent non-native amino acids
    Jeffrey K Takimoto
    The Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, United States
    ACS Chem Biol 6:733-43. 2011
    ..This multifaceted analysis provides a new starting point for engineering PylRS to aminoacylate a significantly more diverse selection of Uaas than previously anticipated...
  4. ncbi request reprint Evolving proteins in mammalian cells using somatic hypermutation
    Lei Wang
    The Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, 10010 N Torrey Pines Road, La Jolla, California 92037, USA
    Nat Protoc 1:1346-50. 2006
    ..For each round of evolution, it takes approximately 1 d to mutate the target gene, approximately 0.5-1 d to select or screen, and 2-4 d to propagate the cells for the next round depending on how many cells are collected...
  5. pmc RF1 knockout allows ribosomal incorporation of unnatural amino acids at multiple sites
    David B F Johnson
    The Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, La Jolla, California, USA
    Nat Chem Biol 7:779-86. 2011
    ..coli tolerates apparent global suppression of UAG. JX33 affords a unique autonomous host for synthesizing and evolving new protein functions by enabling Uaa incorporation at multiple sites...
  6. doi request reprint Human dopamine β-hydroxylase promoter variant alters transcription in chromaffin cells, enzyme secretion, and blood pressure
    Yuqing Chen
    Department of Medicine, University of California at San Diego, La Jolla, USA
    Am J Hypertens 24:24-32. 2011
    ..Here, we characterized a DBH promoter polymorphism (C-2073T; rs1989787; minor allele frequency ~16%) that influences not only gene transcription but also enzyme secretion and blood pressure (BP) in vivo...
  7. doi request reprint Improving orthogonal tRNA-synthetase recognition for efficient unnatural amino acid incorporation and application in mammalian cells
    Jeffrey K Takimoto
    The Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, 10010 N Torrey Pines Road, La Jolla, CA 92037, USA
    Mol Biosyst 5:931-4. 2009
    ....
  8. ncbi request reprint A new strategy for the site-specific modification of proteins in vivo
    Zhiwen Zhang
    Department of Chemistry and Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Biochemistry 42:6735-46. 2003
    ..The genetic incorporation of a nonproteinogenic "ketone handle" into proteins provides a powerful tool for the introduction of biophysical probes for the structural and functional analysis of proteins in vitro or in vivo...
  9. pmc Adding an unnatural covalent bond to proteins through proximity-enhanced bioreactivity
    Zheng Xiang
    The Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, La Jolla, California, USA
    Nat Methods 10:885-8. 2013
    ..We demonstrate the utility of this bond for enabling irreversible binding between an affibody and its protein substrate, capturing peptide-protein interactions in mammalian cells, and improving the photon output of fluorescent proteins. ..
  10. ncbi request reprint Expanding the genetic code
    Lei Wang
    The Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Annu Rev Biophys Biomol Struct 35:225-49. 2006
    ..This methodology provides a powerful tool both for exploring protein structure and function in vitro and in vivo and for generating proteins with new or enhanced properties...
  11. pmc Genetically encoding unnatural amino acids in neural stem cells and optically reporting voltage-sensitive domain changes in differentiated neurons
    Bin Shen
    The Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Stem Cells 29:1231-40. 2011
    ..The method described here should be generally applicable to other stem cells and membrane proteins...
  12. ncbi request reprint An efficient system for the evolution of aminoacyl-tRNA synthetase specificity
    Stephen W Santoro
    Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, SR202, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Nat Biotechnol 20:1044-8. 2002
    ..The new enzymes can be used to produce milligram-per-liter quantities of unnatural amino acid-containing protein in E. coli...
  13. pmc Genetically encoding unnatural amino acids for cellular and neuronal studies
    Wenyuan Wang
    Jack H Skirball Center for Chemical Biology and Proteomics, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Neurosci 10:1063-72. 2007
    ..This technique will stimulate and facilitate new molecular studies using tailored unnatural amino acids for cell biology and neurobiology...
  14. pmc The SRC homology 2 domain protein Shep1 plays an important role in the penetration of olfactory sensory axons into the forebrain
    Lei Wang
    Sanford Burnham Medical Research Institute, La Jolla, California 92037, USA
    J Neurosci 30:13201-10. 2010
    ....
  15. pmc Neuropeptide Y (NPY): genetic variation in the human promoter alters glucocorticoid signaling, yielding increased NPY secretion and stress responses
    Kuixing Zhang
    Department of Medicine and Institute for Genomic Medicine, University of California at San Diego, San Diego, California, USA
    J Am Coll Cardiol 60:1678-89. 2012
    ..This study sought to understand whether genetic variation at the Neuropeptide Y (NPY) locus governs secretion and stress responses in vivo as well as NPY gene expression in sympathochromaffin cells...
  16. pmc Common functional genetic variants in catecholamine storage vesicle protein promoter motifs interact to trigger systemic hypertension
    Kuixing Zhang
    Department of Medicine, University of California at San Diego, San Diego, California, USA
    J Am Coll Cardiol 55:1463-75. 2010
    ....
  17. pmc Human dopamine beta-hydroxylase (DBH) regulatory polymorphism that influences enzymatic activity, autonomic function, and blood pressure
    Yuqing Chen
    Department of Medicine, University of California at San Diego, La Jolla, California 92093 0838, USA
    J Hypertens 28:76-86. 2010
    ..Here we systematically investigated DBH polymorphisms associated with enzymatic activity as well as autonomic and blood pressure (BP)/disease phenotypes in vivo...
  18. ncbi request reprint Amersham Prize winner. Expanding the genetic code
    Lei Wang
    Department of Pharmacology, University of California, San Diego, La Jolla, CA 92093, USA
    Science 302:584-5. 2003
  19. pmc Crystal structures of apo wild-type M. jannaschii tyrosyl-tRNA synthetase (TyrRS) and an engineered TyrRS specific for O-methyl-L-tyrosine
    Yan Zhang
    Department of Molecular Biology, The Scripps Research Institute, 10550 Torrey Pines Road, La Jolla, CA 92037, USA
    Protein Sci 14:1340-9. 2005
    ..The structure of the mutant and wild-type TyrRS now provide a basis for generating new active-site libraries to evolve synthetases specific for other unnatural amino acids...
  20. ncbi request reprint Unnatural amino acid mutagenesis of green fluorescent protein
    Lei Wang
    Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    J Org Chem 68:174-6. 2003
    ..The spectral properties of the mutant GFPs, including the absorbance and fluorescence maxima and quantum yields, correlate with the structural and electronic properties of the substituents on the amino acids...
  21. ncbi request reprint Generation of a bacterium with a 21 amino acid genetic code
    Ryan A Mehl
    Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Am Chem Soc 125:935-9. 2003
    ..This and other such organisms may provide an opportunity to examine the evolutionary consequences of adding new amino acids to the genetic repertoire, as well as generate proteins with new or enhanced biological functions...
  22. ncbi request reprint Expanding the genetic code
    Lei Wang
    Department of Pharmacology, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Angew Chem Int Ed Engl 44:34-66. 2004
    ..By removing the limitations imposed by the existing 20 amino acid code, it should be possible to generate proteins and perhaps entire organisms with new or enhanced properties...
  23. pmc In vivo expression of a light-activatable potassium channel using unnatural amino acids
    Ji Yong Kang
    The Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Neuron 80:358-70. 2013
    ..These principles could be generally expanded to other proteins expressed in the brain to enable optical regulation...
  24. pmc Expanding the genetic code for biological studies
    Qian Wang
    The Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Chem Biol 16:323-36. 2009
    ..Genetic incorporation of unnatural amino acids provides a powerful method for investigating a wide variety of biological processes both in vitro and in vivo...
  25. pmc Enantiospecific synthesis of genetically encodable fluorescent unnatural amino acid L-3-(6-acetylnaphthalen-2-ylamino)-2-aminopropanoic acid
    Zheng Xiang
    The Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    J Org Chem 76:6367-71. 2011
    ..The synthesis takes advantage of a high-yield and enantiospecific Fukuyama-Mitsunobu reaction as the key transformation...
  26. pmc Expanding the genetic code of Caenorhabditis elegans using bacterial aminoacyl-tRNA synthetase/tRNA pairs
    Angela R Parrish
    Jack H Skirball Center for Chemical Biology and Proteomics, Glenn Center for Aging Research, The Salk Institute for Biological Studies, Jolla, California 92037, United States
    ACS Chem Biol 7:1292-302. 2012
    ..We anticipate our strategies will be generally extendable to other multicellular organisms...
  27. pmc Imprints of the genetic code in the ribosome
    David B F Johnson
    The Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 107:8298-303. 2010
    ..The ribosome thus serves as a molecular fossil, preserving biological evidence that anticodon-amino acid interactions shaped the evolution of the genetic code...
  28. pmc Evolution of new nonantibody proteins via iterative somatic hypermutation
    Lei Wang
    Department of Pharmacology, University of California at San Diego, La Jolla, CA 92093 0647, USA
    Proc Natl Acad Sci U S A 101:16745-9. 2004
    ..g., 649 nm), surpassing the best efforts of structure-based design. SHM offers a strategy to evolve nonantibody proteins with desirable properties for which a high-throughput selection or viable single-cell screen can be devised...
  29. doi request reprint New methods enabling efficient incorporation of unnatural amino acids in yeast
    Qian Wang
    Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    J Am Chem Soc 130:6066-7. 2008
    ..These new strategies enabled a significant increase in yield of unnatural amino acid containing proteins from tens of micrograms to tens of milligrams per liter in yeast...
  30. pmc Genetically encoding photoswitchable click amino acids in Escherichia coli and mammalian cells
    Christian Hoppmann
    Chemical Biology and Proteomics Laboratory, The Salk Institute for Biological Studies, 10010 N Torrey Pines Road, La Jolla, CA 92037 USA http wang salk edu
    Angew Chem Int Ed Engl 53:3932-6. 2014
    ..These genetically encodable PSCaas will prove valuable for engineering photoswitchable bridges into proteins for reversible optogenetic regulation. ..
  31. pmc Addition of a photocrosslinking amino acid to the genetic code of Escherichiacoli
    Jason W Chin
    Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 99:11020-4. 2002
    ..Irradiation resulted in efficient crosslinking (>50%) of the protein subunits. This methodology may prove useful for discovering and defining protein interactions in vitro and in vivo...
  32. pmc Photo-cross-linkers incorporated into G-protein-coupled receptors in mammalian cells: a ligand comparison
    Irene Coin
    Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Angew Chem Int Ed Engl 50:8077-81. 2011
    ....
  33. pmc The SH2 domain protein Shep1 regulates the in vivo signaling function of the scaffolding protein Cas
    Séverine Roselli
    Sanford Burnham Medical Research Institute, 10901 N Torrey Pines Rd, La Jolla, CA 92037, USA
    Cell Signal 22:1745-52. 2010
    ..These data demonstrate that Shep1 plays a critical role in the in vivo regulation of Src activity and Cas downstream signaling through Crk, and suggest that the SH2 domain of Shep1 is critical for these effects...
  34. ncbi request reprint Proximity-enabled protein crosslinking through genetically encoding haloalkane unnatural amino acids
    Zheng Xiang
    The Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, 10010 N Torrey Pines Road, La Jolla, CA 92037 USA http wang salk edu
    Angew Chem Int Ed Engl 53:2190-3. 2014
    ....
  35. ncbi request reprint Selective labeling of proteins with chemical probes in living cells
    Michael Z Lin
    Department of Pharmacology, University of California at San Diego, La Jolla, CA, USA
    Physiology (Bethesda) 23:131-41. 2008
    ..Their application in cells and in vivo has yielded novel insights into diverse biological processes...
  36. ncbi request reprint The selective incorporation of alkenes into proteins in Escherichia coli
    Zhiwen Zhang
    Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Angew Chem Int Ed Engl 41:2840-2. 2002
  37. ncbi request reprint A method for the generation of glycoprotein mimetics
    Haitian Liu
    Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    J Am Chem Soc 125:1702-3. 2003
    ..This method may also lead to the production of glycoprotein therapeutics from Escherichia coli...
  38. ncbi request reprint Suppression of in vitro megakaryocyte production by antiplatelet autoantibodies from adult patients with chronic ITP
    Robert McMillan
    The Scripps Research Institute, MEM 215, 10550 N Torrey Pines Rd, La Jolla, CA 92037, USA
    Blood 103:1364-9. 2004
    ..These results show that plasma autoantibody from some adult patients with ITP inhibits in vitro megakaryocyte production, suggesting that a similar effect may occur in vivo...
  39. pmc Expanding the library and substrate diversity of the pyrrolysyl-tRNA synthetase to incorporate unnatural amino acids containing conjugated rings
    Vanessa K Lacey
    Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, 10010 N Torrey Pines Road, La Jolla, CA 92037 USA
    Chembiochem 14:2100-5. 2013
    ..Using the "small-intelligent" approach to construct a highly diverse library, we evolved novel synthetases specific for two such UAAs and incorporated them into proteins in E. coli and mammalian cells. ..
  40. pmc Neuropeptide Y(1) Receptor NPY1R discovery of naturally occurring human genetic variants governing gene expression in cella as well as pleiotropic effects on autonomic activity and blood pressure in vivo
    Lei Wang
    Department of Medicine, University of California at San Diego, California, USA
    J Am Coll Cardiol 54:944-54. 2009
    ..We asked whether naturally occurring genetic variation at the human NPY1R locus alters autonomic traits that might predispose individuals to cardiovascular disease...
  41. pmc Bacterial inclusion bodies contain amyloid-like structure
    Lei Wang
    Structural Biology Laboratory, The Salk Institute, La Jolla, California, United States of America
    PLoS Biol 6:e195. 2008
    ..These findings suggest that inclusion bodies are structured, that amyloid formation is an omnipresent process both in eukaryotes and prokaryotes, and that amino acid sequences evolve to avoid the amyloid conformation...
  42. pmc Glial ephrin-A3 regulates hippocampal dendritic spine morphology and glutamate transport
    Maria A Carmona
    Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 106:12524-9. 2009
    ..Our results suggest that the interaction between neuronal EphA4 and glial ephrin-A3 bidirectionally controls synapse morphology and glial glutamate transport, ultimately regulating hippocampal function...
  43. pmc Genetically encoded chemical probes in cells reveal the binding path of urocortin-I to CRF class B GPCR
    Irene Coin
    Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Cell 155:1258-69. 2013
    ..Structural features of the receptor-ligand complex yield molecular insights on the mechanism of receptor activation and the basis for discrimination between agonist and antagonist function...
  44. ncbi request reprint Manipulating proteins for neuroscience
    Angela R Parrish
    The Jack H Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037 1099 USA
    Curr Opin Neurobiol 16:585-92. 2006
    ....
  45. pmc A common genetic variant in the 3'-UTR of vacuolar H+-ATPase ATP6V0A1 creates a micro-RNA motif to alter chromogranin A processing and hypertension risk
    Zhiyun Wei
    Departments of Medicine and Pharmacology, Institute for Genomic Medicine, University of California at San Diego, the VA San Diego Healthcare System, La Jolla, USA
    Circ Cardiovasc Genet 4:381-9. 2011
    ..We explored the molecular mechanism of this effect by experiments with transfected chimeric photoproteins in chromaffin cells...
  46. pmc Hepatic vascular endothelial growth factor regulates recruitment of rat liver sinusoidal endothelial cell progenitor cells
    Lin Wang
    Division of Gastrointestinal and Liver Disease and the University of Southern California Research Center for Liver Disease, Keck School of Medicine, University of Southern California, Los Angeles, California, USA
    Gastroenterology 143:1555-1563.e2. 2012
    ..We examined how hepatic vascular endothelial growth factor (VEGF) regulates recruitment of BM SPCs and their effects on liver injury...
  47. ncbi request reprint Many alphaIIbbeta3 autoepitopes in chronic immune thrombocytopenic purpura are localized to alphaIIb between amino acids L1 and Q459
    Robert McMillan
    The Scripps Research Institute, La Jolla, CA 92037, USA
    Br J Haematol 118:1132-6. 2002
    ..We conclude that the binding of many anti-alphaIIbbeta3 autoantibodies is dependent on the presence of alphaIIb amino acids L1-Q459...
  48. pmc Regulation of adult stem cell behavior by nutrient signaling
    Lei Wang
    Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, CA, USA
    Cell Cycle 10:2628-34. 2011
    ..Here we discuss our data regarding the effects and mechanisms by which changes in systemic nutritional conditions may influence the maintenance and activity of adult stem cells via insulin signaling...
  49. pmc Transnitrosylation of XIAP regulates caspase-dependent neuronal cell death
    Tomohiro Nakamura
    Del E Webb Center for Neuroscience, Aging, and Stem Cell Research, Sanford Burnham Medical Research Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
    Mol Cell 39:184-95. 2010
    ..These findings provide insights into the regulation of caspase activation in neurodegenerative disorders mediated, at least in part, by nitrosative stress...
  50. pmc Addition of the keto functional group to the genetic code of Escherichia coli
    Lei Wang
    Department of Chemistry, University of California, Berkeley 94720, USA
    Proc Natl Acad Sci U S A 100:56-61. 2003
    ..This additional genetically encoded amino acid should greatly expand our ability to manipulate protein structure and function both in vitro and in living cells...
  51. ncbi request reprint Regulatory properties of alpha(1B)-adrenergic receptors defective in coupling to phosphoinositide hydrolysis
    Jiefa Wang
    Department of Pharmacology, University of Nebraska Medical Center, Omaha, Nebraska 68198 6260, USA
    J Pharmacol Exp Ther 300:134-41. 2002
    ....
  52. ncbi request reprint Proteomic analysis of effect of hyperthermia on spermatogenesis in adult male mice
    Ye fei Zhu
    Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, PR China
    J Proteome Res 5:2217-25. 2006
    ..Further analysis in this heat-shocked model suggests numerous potential mechanisms for heat shock-induced spermatogenic disorder...
  53. pmc Extensive variation in the O-antigen gene cluster within one Salmonella enterica serogroup reveals an unexpected complex history
    Lei Wang
    Department of Microbiology, The University of Sydney, Sydney, New South Wales 2006, Australia
    J Bacteriol 184:1669-77. 2002
    ..Two of the forms lacking a functional wzy(alpha(1-6)) gene have the wzy(alpha(1-2)) gene first described for strain LT2 as rfc, whereas for the third the wzy gene has not been located...
  54. ncbi request reprint p19Arf-p53 tumor suppressor pathway regulates cell motility by suppression of phosphoinositide 3-kinase and Rac1 GTPase activities
    Fukun Guo
    Division of Experimental Hematology, Children s Hospital Research Foundation, University of Cincinnati, Ohio 45229, USA
    J Biol Chem 278:14414-9. 2003
    ....
  55. pmc A preliminary analysis of genome structure and composition in Gossypium hirsutum
    Wangzhen Guo
    National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, PR China
    BMC Genomics 9:314. 2008
    ..Here, we employed GeneTrek and BAC tagging information approaches to predict the general composition and structure of the allotetraploid cotton genome...
  56. ncbi request reprint A novel strategy for specifically down-regulating individual Rho GTPase activity in tumor cells
    Lei Wang
    Division of Experimental Hematology and Molecular Developmental Biology Program, Children s Hospital Research Foundation, University of Cincinnati, Cincinnati, Ohio 45229, USA
    J Biol Chem 278:44617-25. 2003
    ....
  57. ncbi request reprint Amphioxus allantoicase: molecular cloning, expression and enzymatic activity
    Yongjun Wang
    Department of Marine Biology, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China
    Comp Biochem Physiol B Biochem Mol Biol 141:237-43. 2005
    ..These suggest that allantoicase remains in action in the primitive chordate amphioxus...
  58. ncbi request reprint Identification and expression of amphioxus beta-microseminoprotein (MSP)-like gene encoding an ancient and rapidly evolving protein in chordates
    Yongjun Wang
    Department of Marine Biology, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China
    Comp Biochem Physiol B Biochem Mol Biol 142:251-7. 2005
    ....
  59. ncbi request reprint [Sequence of Escherichia coli O141 O-antigen gene cluster and analyses of its evolutionary history]
    Qing Ke Kong
    College of Life Science, Nankai University, Tianjin 300457, China
    Yi Chuan Xue Bao 31:1448-54. 2004
    ..We find that these genes are typical E. coli genes and might have been involved in recombination events between O-antigen gene clusters...
  60. ncbi request reprint Diversity and evolution of conotoxins based on gene expression profiling of Conus litteratus
    Canhui Pi
    Department of Biochemistry, College of Life Sciences, Sun Yat Sen Zhongshan University, Guangzhou 510275, People s Republic of China
    Genomics 88:809-19. 2006
    ..These results give an initial insight into the C. litteratus transcriptome that will contribute to a better understanding of conotoxin evolution and the study of the cone snail genome in the near future...
  61. ncbi request reprint Characterization of E. coli O24 and O56 O antigen gene clusters reveals a complex evolutionary history of the O24 gene cluster
    Jiansong Cheng
    TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 HongDa Street, Tianjin, TEDA, 300457, P R China
    Curr Microbiol 53:470-6. 2006
    ..Genes specific to E. coli O24 and O56 were also identified, which may be used for development of DNA-based serotyping schemes...
  62. ncbi request reprint Cloning and characterization of a novel sperm tail protein, NYD-SP28
    Ying Zheng
    Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing 10029, PR China
    Int J Mol Med 18:1119-25. 2006
    ..In conclusion, these results suggest that NYD-SP28 is a new human sperm tail protein and might play an important role during sperm capacitation...
  63. ncbi request reprint Quantification of protein based on single-molecule counting by total internal reflection fluorescence microscopy with adsorption equilibrium
    Lei Wang
    School of Chemistry and Chemical Engineering, Shandong University, Jinan, PR China
    Anal Chim Acta 590:104-9. 2007
    ..The spot number showed an excellent linear relationship with protein concentration. The concentration linear range was 5.4 x 10(-11) to 8.1 x 10(-10) mol L(-1)...
  64. ncbi request reprint Salt-tolerance genes involved in cation efflux and osmoregulation of Sinorhizobium fredii RT19 detected by isolation and characterization of Tn5 mutants
    Ju Quan Jiang
    Department of Microbiology, College of Biological Sciences, China Agricultural University and Key Laboratory of Agro Microbial Resource and Application, Ministry of Agriculture, No 2 Yuanmingyuan West Road, Beijing, 100094, PR China
    FEMS Microbiol Lett 239:139-46. 2004
    ..fredii RT19...
  65. ncbi request reprint The site-specific incorporation of p-iodo-L-phenylalanine into proteins for structure determination
    Jianming Xie
    Nat Biotechnol 22:1297-301. 2004
    ..The ability to selectively introduce this and other heavy atom-containing amino acids into proteins should facilitate the structural study of proteins...
  66. ncbi request reprint Transcriptional profiling of human embryonic stem cells and embryoid bodies identifies HESRG, a novel stem cell gene
    Ming Zhao
    Cancer Research Institute, Xiang Ya School of Medicine, Central South University, 110 Xiangya Road, Changsha 410078, Hunan, and Department of Neurosurgery, Xiangya Hospital, China
    Biochem Biophys Res Commun 362:916-22. 2007
    ..The above suggested an important role for the novel HESRG gene in supporting the undifferentiated state or self-renewal of hES cells...
  67. ncbi request reprint Neural progenitor cell transplantation and imaging in a large animal model
    Lei Wang
    Scott Ritchey Research Center, College of Veterinary Medicine, Auburn University, Auburn, AL 36849 5525, United States
    Neurosci Res 59:327-40. 2007
    ..The characterization of fNPCs and exploration of non-invasive bioluminescent imaging to track transplanted cells in this study will allow evaluation of NPC transplantation therapy using feline models of human neurological diseases...
  68. ncbi request reprint Identification of a type III thioesterase reveals the function of an operon crucial for Mtb virulence
    Feng Wang
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843, USA
    Chem Biol 14:543-51. 2007
    ..The characterization of Mtb FcoT provides a putative function of this operon that is crucial for Mtb pathogenicity...
  69. ncbi request reprint High-level secretory production of recombinant bovine enterokinase light chain by Pichia pastoris
    Lisheng Peng
    The Open Laboratory for Marine Functional Genomics of State High Tech Development, Department of Biochemistry, College of Life Sciences, Sun Yat Sen Zhongshan University, Guangzhou 510275, PR China
    J Biotechnol 108:185-92. 2004
    ..pastoris, and this His-tagged EK(L) exhibited a similar enzymatic activity to the untagged EK(L)...
  70. ncbi request reprint Stationary phase protein overproduction is a fundamental capability of Escherichia coli
    Jingxing Ou
    Key Laboratory of Genetic Engineering of Ministry of Education, Department of Biochemistry, College of Life Sciences, Sun Yat Sen Zhongshan University, Guangzhou, People s Republic of China
    Biochem Biophys Res Commun 314:174-80. 2004
    ..These results not only provide a better strategy for recombinant protein preparation in E. coli, but also reveal that rapid rehabilitation from stresses and stationary phase protein overproduction are fundamental characters of E. coli...
  71. pmc Structural and genetic characterization of the Shigella boydii type 10 and type 6 O antigens
    Sof ya N Senchenkova
    N D Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation
    J Bacteriol 187:2551-4. 2005
    ..Therefore, it is proposed that the O-antigen gene cluster of S. boydii type 6 was derived from type 10 by an IS element insertion...