S J Shattil

Summary

Affiliation: The Scripps Research Institute
Country: USA

Publications

  1. ncbi request reprint Not just another pretty face: regulation of platelet function at the cytoplasmic face of integrin alpha IIb beta 3
    S J Shattil
    Department of Vascular Biology, Scripps Research Institute, La Jolla, CA 92037, USA
    Thromb Haemost 78:220-5. 1997
  2. ncbi request reprint Signaling through platelet integrin alpha IIb beta 3: inside-out, outside-in, and sideways
    S J Shattil
    Department of Vascular Biology, Scripps Research Institute, La Jolla, CA 92037, USA
    Thromb Haemost 82:318-25. 1999
  3. ncbi request reprint All in the family: primary megakaryocytes for studies of platelet alphaIIbbeta3 signaling
    S J Shattil
    Department of Vascular Biology, Scripps Research Institute, La Jolla, CA 92037, USA
    Thromb Haemost 86:259-65. 2001
  4. ncbi request reprint Activation of Syk protein tyrosine kinase through interaction with integrin beta cytoplasmic domains
    D G Woodside
    Department of Vascular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Curr Biol 11:1799-804. 2001
  5. pmc Regulation of the pp72syk protein tyrosine kinase by platelet integrin alpha IIb beta 3
    J Gao
    Department of Vascular Biology, Scripps Research Institute, La Jolla, CA 92037, USA
    EMBO J 16:6414-25. 1997
  6. ncbi request reprint Rac recruits high-affinity integrin alphavbeta3 to lamellipodia in endothelial cell migration
    W B Kiosses
    Department of Vascular Biology, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Cell Biol 3:316-20. 2001
  7. ncbi request reprint A role for PKCtheta in outside-in alpha(IIb)beta3 signaling
    A Soriani
    Division of Hematology Oncology, Department of Medicine, University of California San Diego, La Jolla, CA, USA
    J Thromb Haemost 4:648-55. 2006
  8. pmc What is vinculin needed for in platelets?
    J V Mitsios
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093 0726, USA
    J Thromb Haemost 8:2294-304. 2010
  9. pmc Integrin activation controls metastasis in human breast cancer
    B Felding-Habermann
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 98:1853-8. 2001
  10. doi request reprint The beta3 integrin cytoplasmic tail: protein scaffold and control freak
    S J Shattil
    Division of Hematology Oncology, Department of Medicine, University of California San Diego, La Jolla, CA 92093 0726, USA
    J Thromb Haemost 7:210-3. 2009

Collaborators

Detail Information

Publications41

  1. ncbi request reprint Not just another pretty face: regulation of platelet function at the cytoplasmic face of integrin alpha IIb beta 3
    S J Shattil
    Department of Vascular Biology, Scripps Research Institute, La Jolla, CA 92037, USA
    Thromb Haemost 78:220-5. 1997
    ....
  2. ncbi request reprint Signaling through platelet integrin alpha IIb beta 3: inside-out, outside-in, and sideways
    S J Shattil
    Department of Vascular Biology, Scripps Research Institute, La Jolla, CA 92037, USA
    Thromb Haemost 82:318-25. 1999
  3. ncbi request reprint All in the family: primary megakaryocytes for studies of platelet alphaIIbbeta3 signaling
    S J Shattil
    Department of Vascular Biology, Scripps Research Institute, La Jolla, CA 92037, USA
    Thromb Haemost 86:259-65. 2001
    ....
  4. ncbi request reprint Activation of Syk protein tyrosine kinase through interaction with integrin beta cytoplasmic domains
    D G Woodside
    Department of Vascular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Curr Biol 11:1799-804. 2001
    ..Thus, the direct binding of Syk kinase to the integrin beta3 cytoplasmic tail is a novel and functionally significant mechanism for the regulation of this important non-receptor tyrosine kinase...
  5. pmc Regulation of the pp72syk protein tyrosine kinase by platelet integrin alpha IIb beta 3
    J Gao
    Department of Vascular Biology, Scripps Research Institute, La Jolla, CA 92037, USA
    EMBO J 16:6414-25. 1997
    ..Furthermore, there are fundamental differences in the activation of Syk by alpha IIb beta 3 and immune response receptors, suggesting a unique role for integrins in Syk function...
  6. ncbi request reprint Rac recruits high-affinity integrin alphavbeta3 to lamellipodia in endothelial cell migration
    W B Kiosses
    Department of Vascular Biology, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Cell Biol 3:316-20. 2001
    ..By using WOW-1, we have identified the selective recruitment of high-affinity integrins as a mechanism by which lamellipodia promote formation of new adhesions at the leading edge in cell migration...
  7. ncbi request reprint A role for PKCtheta in outside-in alpha(IIb)beta3 signaling
    A Soriani
    Division of Hematology Oncology, Department of Medicine, University of California San Diego, La Jolla, CA, USA
    J Thromb Haemost 4:648-55. 2006
    ..Thus, PKCtheta is a newly identified, essential member of a dynamic outside-in signaling complex that includes Btk and that couples alpha(IIb)beta3 to the actin cytoskeleton...
  8. pmc What is vinculin needed for in platelets?
    J V Mitsios
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093 0726, USA
    J Thromb Haemost 8:2294-304. 2010
    ..Conclusion: Despite promoting membrane cytoskeleton integrity when mechanical force is applied to α(IIb) β(3) , vinculin is not required for the traditional functions of α(IIb) β(3) or the platelet actin cytoskeleton...
  9. pmc Integrin activation controls metastasis in human breast cancer
    B Felding-Habermann
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 98:1853-8. 2001
    ..Consequently, alterations within tumors that lead to the aberrant control of integrin activation are expected to adversely affect the course of human breast cancer...
  10. doi request reprint The beta3 integrin cytoplasmic tail: protein scaffold and control freak
    S J Shattil
    Division of Hematology Oncology, Department of Medicine, University of California San Diego, La Jolla, CA 92093 0726, USA
    J Thromb Haemost 7:210-3. 2009
    ..We speculate that this scaffold or its interacting proteins may serve as therapeutic targets for the development of future anti-thrombotic drugs...
  11. ncbi request reprint Signaling networks linking integrins and rho family GTPases
    M A Schwartz
    Dept of Vascular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Trends Biochem Sci 25:388-91. 2000
    ..Recently, it has also become apparent that integrins regulate Rho GTPases and vice versa. Integrins and GTPases might therefore be organized into complex signaling cascades that regulate cell behavior...
  12. pmc Activation of integrins in endothelial cells by fluid shear stress mediates Rho-dependent cytoskeletal alignment
    E Tzima
    Department of Vascular Biology, The Scripps Research Institute, 10550 N.Torrey Pines Road, La Jolla, CA 92037, USA
    EMBO J 20:4639-47. 2001
    ..This transient inhibition is necessary for cytoskeletal alignment in the direction of flow. The results therefore define the role of integrins and Rho in a pathway leading to endothelial cell adaptation to flow...
  13. pmc Affinity modulation of platelet integrin alphaIIbbeta3 by beta3-endonexin, a selective binding partner of the beta3 integrin cytoplasmic tail
    H Kashiwagi
    Department of Vascular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Cell Biol 137:1433-43. 1997
    ..By analogy, the adhesive function of platelets may be regulated by such protein-protein interactions at the level of the cytoplasmic tails of alphaIIbbeta3...
  14. pmc EGFR-dependent pancreatic carcinoma cell metastasis through Rap1 activation
    M Huang
    Department of Pathology, Moores University of California San Diego Cancer Center, La Jolla, CA 92093 1503, USA
    Oncogene 31:2783-93. 2012
    ..These findings identify a molecular pathway that promotes the invasive/metastatic properties of human pancreatic carcinomas driven by EGFR...
  15. pmc Mechanisms and consequences of agonist-induced talin recruitment to platelet integrin alphaIIbbeta3
    Naohide Watanabe
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 181:1211-22. 2008
    ..Thus, talin recruitment to alphaIIbbeta3 by RIAM mediates agonist-induced alphaIIbbeta3 activation, with implications for hemostasis and thrombosis...
  16. pmc PTP-1B is an essential positive regulator of platelet integrin signaling
    Elena Garcia Arias-Salgado
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 170:837-45. 2005
    ..Thus, PTP-1B is an essential positive regulator of the initiation of outside-in alphaIIbbeta3 signaling in platelets...
  17. ncbi request reprint Caspase-12: a developmental link between G-protein-coupled receptors and integrin alphaIIbbeta3 activation
    Steven W Kerrigan
    Department of Laboratory Medicine, University of California San Francisco, 513 Parnassus Ave, Rm S 577, San Francisco, CA 94143 0100, USA
    Blood 104:1327-34. 2004
    ....
  18. ncbi request reprint Signaling through GP Ib-IX-V activates alpha IIb beta 3 independently of other receptors
    Ana Kasirer-Friede
    Department of Cell Biology, The Scripps Research Institute, 10550 N Torrey Pines Rd, La Jolla, CA 92037, USA
    Blood 103:3403-11. 2004
    ..These data establish that GP Ib-IX-V itself can signal to activate alpha IIb beta 3, through sequential actions of Src kinases, Ca(2+) oscillations, and PI 3-kinase/PKC...
  19. ncbi request reprint Lateral clustering of platelet GP Ib-IX complexes leads to up-regulation of the adhesive function of integrin alpha IIbbeta 3
    Ana Kasirer-Friede
    Departments of Cell Biology and Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 277:11949-56. 2002
    ..By extrapolation, GP Ib-IX clustering in platelets may promote thrombus formation...
  20. ncbi request reprint Relationships between Rap1b, affinity modulation of integrin alpha IIbbeta 3, and the actin cytoskeleton
    Alessandra Bertoni
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 277:25715-21. 2002
    ..These studies establish that Rap1b can augment agonist-induced ligand binding to alpha(IIb)beta(3) through effects on integrin affinity, possibly by modulating alpha(IIb)beta(3) interactions with the actin cytoskeleton...
  21. pmc Differential role of glycolipid-enriched membrane domains in glycoprotein VI- and integrin-mediated phospholipase Cgamma2 regulation in platelets
    Peter Wonerow
    Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, UK
    Biochem J 364:755-65. 2002
    ....
  22. pmc Coordinate interactions of Csk, Src, and Syk kinases with [alpha]IIb[beta]3 initiate integrin signaling to the cytoskeleton
    Achim Obergfell
    Division of Vascular Biology, Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Biol 157:265-75. 2002
    ..These studies establish that platelet spreading on fibrinogen requires sequential activation of Src and Syk in proximity to alphaIIbbeta3, thus providing a paradigm for initiation of integrin signaling to the actin cytoskeleton...
  23. ncbi request reprint Talin binding to integrin beta tails: a final common step in integrin activation
    Seiji Tadokoro
    Department of Cell Biology, The Scripps Research Institute, The Burnham Institute, La Jolla, CA 92037, USA
    Science 302:103-6. 2003
    ..Thus, regulated binding of talin to integrin beta tails is a final common element of cellular signaling cascades that control integrin activation...
  24. pmc The antithrombotic potential of selective blockade of talin-dependent integrin alpha IIb beta 3 (platelet GPIIb-IIIa) activation
    Brian G Petrich
    Department of Medicine, UCSD School of Medicine, La Jolla, California 92093 0726, USA
    J Clin Invest 117:2250-9. 2007
    ..Furthermore, they suggest that modulation of beta(3) integrin-talin interactions may provide an attractive target for antithrombotics and result in a reduced risk of pathological bleeding...
  25. ncbi request reprint Platelet integrin adhesive functions and signaling
    Nicolas Prevost
    Division of Hematology Oncology, Department of Medicine, University of California, San Diego, La Jolla, California, USA
    Methods Enzymol 426:103-15. 2007
    ..The present chapter details the various protocols and reagents currently in use in our laboratory to study alphaIIbbeta3 adhesive responses and signaling in both human and murine cell models...
  26. pmc Roles of focal adhesion kinase (FAK) in megakaryopoiesis and platelet function: studies using a megakaryocyte lineage specific FAK knockout
    Ian S Hitchcock
    Department of Medicine, University of California San Diego, La Jolla 92093, USA
    Blood 111:596-604. 2008
    ..These studies establish clear roles for FAK in megakaryocyte growth and platelet function, setting the stage for manipulation of this component of the Tpo signaling apparatus for therapeutic benefit...
  27. pmc Microfluidic devices for studies of shear-dependent platelet adhesion
    Edgar Gutierrez
    Department of Physics, University of California San Diego, La Jolla, CA 92093, USA
    Lab Chip 8:1486-95. 2008
    ..Because of small sample volumes required, the devices could be employed in research with genetically-modified model organisms and for adhesion tests in clinical settings with blood from neonates...
  28. pmc The GPIIb/IIIa (integrin alphaIIbbeta3) odyssey: a technology-driven saga of a receptor with twists, turns, and even a bend
    Barry S Coller
    Laboratory of Blood and Vascular Biology, The Rockefeller University, New York, NY, USA
    Blood 112:3011-25. 2008
    ....
  29. pmc Differential requirement for LAT and SLP-76 in GPVI versus T cell receptor signaling
    Barbi A Judd
    Program in Molecular Biology, University of Iowa, Iowa City, IA 52242, USA
    J Exp Med 195:705-17. 2002
    ....
  30. ncbi request reprint Platelet integrins and immunoreceptors
    Ana Kasirer-Friede
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093 0726, USA
    Immunol Rev 218:247-64. 2007
    ....
  31. pmc The oligodendrocyte precursor mitogen PDGF stimulates proliferation by activation of alpha(v)beta3 integrins
    Wia Baron
    Department of Medical Genetics, University of Cambridge, and Cambridge Centre for Brain Repair, ED Adrian Building, University Forvie Site, Cambridge CB2 2PY, UK
    EMBO J 21:1957-66. 2002
    ....
  32. ncbi request reprint Reconstructing and deconstructing agonist-induced activation of integrin alphaIIbbeta3
    Jaewon Han
    Department of Medicine, University of California, San Diego, La Jolla, California 92093, USA
    Curr Biol 16:1796-806. 2006
    ..Many different signaling molecules that regulate integrin affinity have been described, but a pathway that connects agonist stimulation to talin binding and activation has not been mapped...
  33. pmc Src kinase activation by direct interaction with the integrin beta cytoplasmic domain
    Elena G Arias-Salgado
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 100:13298-302. 2003
    ..The data provide a paradigm for integrin regulation of Src and a molecular basis for the similar functional defects of osteoclasts or platelets from mice lacking beta3 integrins or c-Src...
  34. ncbi request reprint Detection of integrin alpha IIbbeta 3 clustering in living cells
    Charito Buensuceso
    Departments of Cell Biology and Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 278:15217-24. 2003
    ..These studies in living cells establish that alpha(IIb)beta(3) clustering is modulated by fibrinogen and actin dynamics. More broadly, they should facilitate investigations of the mechanisms and consequences of integrin clustering...
  35. pmc Megakaryocytes derived from embryonic stem cells implicate CalDAG-GEFI in integrin signaling
    Koji Eto
    Departments of Cell Biology and Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 99:12819-24. 2002
    ..01). These results establish that ES cells are a ready source of mature megakaryocytes for integrin studies and other biological applications, and they implicate CalDAG-GEFI in inside-out signaling to alphaIIbbeta3...
  36. pmc Proximal, selective, and dynamic interactions between integrin alphaIIbbeta3 and protein tyrosine kinases in living cells
    Maddalena de Virgilio
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Rd, VB 5, La Jolla, CA 92037, USA
    J Cell Biol 165:305-11. 2004
    ..Thus, alphaIIbbeta3 interacts proximally, if not directly, with tyrosine kinases in a coordinated, selective, and dynamic manner during sequential phases of alphaIIbbeta3 signaling to the actin cytoskeleton...
  37. ncbi request reprint Integrins: dynamic scaffolds for adhesion and signaling in platelets
    Sanford J Shattil
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA, USA
    Blood 104:1606-15. 2004
    ....
  38. ncbi request reprint Protein-protein interactions in platelet alphaIIbbeta3 signaling
    Charito S Buensuceso
    Division of Hematology Oncology, Department of Medicine, University of California San Diego, La Jolla, California 92093, USA
    Semin Thromb Hemost 30:427-39. 2004
    ..This review summarizes the known proteins that directly interact with alpha (IIb)bbeta (3) and provides an overview of their roles in integrin function...
  39. ncbi request reprint Regulation of outside-in signaling in platelets by integrin-associated protein kinase C beta
    Charito S Buensuceso
    Hematology Oncology Division, Department of Medicine, University of California San Diego, La Jolla, California 92093, USA
    J Biol Chem 280:644-53. 2005
    ..Furthermore, the studies extend the concept of alpha(IIb)beta(3) as a scaffold for multiple protein kinases that regulate the platelet actin cytoskeleton...
  40. ncbi request reprint Amino acid changes in Drosophila alphaPS2betaPS integrins that affect ligand affinity
    Thomas A Bunch
    Department of Molecular and Cellular Biology, Arizona Cancer Center, Tucson, Arizona 85724, USA
    J Biol Chem 281:5050-7. 2006
    ..These studies establish a means to evaluate mechanisms and consequences of integrin affinity modulation in a tractable model genetic system...
  41. pmc ADAP is required for normal alphaIIbbeta3 activation by VWF/GP Ib-IX-V and other agonists
    Ana Kasirer-Friede
    Department of Medicine, University of California San Diego, La Jolla 92093 0726, USA
    Blood 109:1018-25. 2007
    ..These studies establish ADAP as a component of inside-out signaling pathways that couple GP Ib-IX-V and other platelet agonist receptors to alphaIIbbeta3 activation...