Research Topics
| Eduard SergienkoSummaryAffiliation: The Burnham Institute Country: USA Publications
| Collaborators
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Detail Information
Publications
Identification and characterization of novel tissue-nonspecific alkaline phosphatase inhibitors with diverse modes of actionEduard Sergienko
Conrad Prebys Center for Chemical Genomics, Burnham Institute for Medical Research, La Jolla, California 92037, USA
J Biomol Screen 14:824-37. 2009....
High-throughput screening of tissue-nonspecific alkaline phosphatase for identification of effectors with diverse modes of actionEduard A Sergienko
Conrad Prebys Center for Chemical Genomics, Sanford Burnham Medical Research Institute, La Jolla, California, USA
Nat Protoc 5:1431-9. 2010..This protocol provides a method of screening TNAP that is 1,000-fold more sensitive and 10-fold faster than a conventional colorimetric assay with p-nitrophenyl phosphate...
High-throughput fluorescence polarization assay for chemical library screening against anti-apoptotic Bcl-2 family member Bfl-1Dayong Zhai
Sanford Burnham Medical Research Institute, Program on Apoptosis and Cell Death Research, La Jolla, CA 92037, USA
J Biomol Screen 17:350-60. 2012..Collectively, these findings demonstrate the feasibility of using the HTS assay for discovery of selective chemical inhibitors of Bfl-1...
Design and synthesis of pyrazole derivatives as potent and selective inhibitors of tissue-nonspecific alkaline phosphatase (TNAP)Shyama Sidique
Burnham Center for Chemical Genomics, Burnham Institute for Medical Research, La Jolla, CA 92037, USA
Bioorg Med Chem Lett 19:222-5. 2009..A representative of the series was characterized in kinetic studies and determined to have a mode of inhibition not previously observed for TNAP inhibitors...
Inhibition of hematopoietic protein tyrosine phosphatase augments and prolongs ERK1/2 and p38 activationEduard Sergienko
Infectious and Inflammatory Disease Center, Sanford Burnham Medical Research Institute, La Jolla, California 92037, United States
ACS Chem Biol 7:367-77. 2012..Our results warrant future studies with this probe compound that may establish HePTP as a new drug target for acute leukemic conditions...
Assay format as a critical success factor for identification of novel inhibitor chemotypes of tissue-nonspecific alkaline phosphatase from high-throughput screeningThomas D Y Chung
Conrad Prebys Center for Chemical Genomics, Sanford Burnham Medical Research Institute, La Jolla, CA 92037, USA
Molecules 15:3010-37. 2010..This illustrates the underappreciated impact of the underlying fundamental assay configuration on screening success, beyond mere signal generation and detection formats...
High-throughput fluorescence assay for small-molecule inhibitors of autophagins/Atg4Chih Wen Shu
Sanford Burnham Medical Research Institute, Program on Apoptosis and Cell Death Research, and Conrad Prebys Center for Chemical Genomics, La Jolla, CA 92037, USA
J Biomol Screen 16:174-82. 2011....
Potent, selective, and orally available benzoisothiazolone phosphomannose isomerase inhibitors as probes for congenital disorder of glycosylation IaRussell Dahl
Apoptosis and Cell Death Research Program, Conrad Prebys Center for Chemical Genomics, Sanford Burnham Medical Research Institute, 10901 North Torrey Pines Road, La Jolla, California 92037, United States
J Med Chem 54:3661-8. 2011..The pharmacokinetic profile of a representative of this series (compound 19; ML089) was also assessed, demonstrating the potential of this series for in vivo efficacy when dosed orally in disease models...
Discovery of 4-oxo-6-((pyrimidin-2-ylthio)methyl)-4H-pyran-3-yl 4-nitrobenzoate (ML221) as a functional antagonist of the apelin (APJ) receptorPatrick R Maloney
Conrad Prebys Center for Chemical Genomics, Sanford Burnham Medical Research Institute, 6400 Sanger Road, Orlando, FL 32827, USA
Bioorg Med Chem Lett 22:6656-60. 2012..The synthetic methodology, development of structure-activity relationship (SAR), and initial in vitro pharmacologic characterization are also presented...
Discovery and characterization of 2-aminobenzimidazole derivatives as selective NOD1 inhibitorsRicardo G Correa
Sanford Burnham Medical Research Institute, La Jolla, CA 92037, USA
Chem Biol 18:825-32. 2011..Altogether, this inaugural class of inhibitors provides chemical probes for interrogating mechanisms regulating NOD1 activity and tools for exploring the roles of NOD1 in various infectious and inflammatory diseases...
Versatile assays for high throughput screening for activators or inhibitors of intracellular proteases and their cellular regulatorsHideki Hayashi
Burnham Institute for Medical Research, La Jolla, California, USA
PLoS ONE 4:e7655. 2009..methods #entitystartx00026;..
A TR3/Nur77 peptide-based high-throughput fluorescence polarization screen for small molecule Bcl-B inhibitorsKenneth W Yip
Burnham Institute for Medical Research, La Jolla, California 92037, USA
J Biomol Screen 13:665-73. 2008..Experiments using Bcl-B overexpressing HeLa cells demonstrated that this compound induced Bcl-B-dependent cell death. The current FPA represents a screen that can identify noncanonical inhibitors of Bcl-2-family proteins...
