Research Topics
| S L SchmidSummaryAffiliation: The Scripps Research Institute Country: USA Publications
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Detail Information
Publications
Control of EGF receptor signaling by clathrin-mediated endocytosisA V Vieira
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Science 274:2086-9. 1996..Thus, endocytic trafficking of activated EGFR plays a critical role not only in attenuating EGFR signaling but also in establishing and controlling specific signaling pathways...
Dynamin: functional design of a membrane fission catalystSandra L Schmid
Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
Annu Rev Cell Dev Biol 27:79-105. 2011....- Days and knights discussing membrane dynamics in endocytosis: meeting report from the Euresco/EMBL Membrane Dynamics in Endocytosis, 6--11 October in Tomar, PortugalSandra L Schmid
The Scripps Research Institute, La Jolla, CA 92037, USA
Traffic 3:77-85. 2002.... 
The Palade symposium: celebrating cell biology at its bestSandra L Schmid
Department of Cell Biology, The Scripps Research Institute, University of California, San Diego, La Jolla, CA 92037, USA
Mol Biol Cell 21:2367-70. 2010....- Receptor and membrane recycling can occur with unaltered efficiency despite dramatic Rab5(q79l)-induced changes in endosome geometryB P Ceresa
Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
J Biol Chem 276:9649-54. 2001..These results suggest that GTP hydrolysis by Rab5 is rate-limiting for endosome fusion but not for endocytic trafficking and that early endosome geometry may be a less critical determinant of sorting efficiencies than previously thought... - Dynamin GTPase, a force-generating molecular switchD E Warnock
Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92037, USA
Bioessays 18:885-93. 1996..In this case, dynamin would be the first GTPase identified that acts as a structural protein with mechano-chemical function... - Dominant-interfering Hsc70 mutants disrupt multiple stages of the clathrin-coated vesicle cycle in vivoS L Newmyer
Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
J Cell Biol 152:607-20. 2001..Our findings demonstrate that hsc70 indeed regulates coat disassembly and also suggest that this chaperone broadly modulates clathrin dynamics throughout the CCV cycle... - Dynamin GTPase domain mutants block endocytic vesicle formation at morphologically distinct stagesH Damke
Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
Mol Biol Cell 12:2578-89. 2001..Nonetheless, we find that overexpression of dynamin mutants with subtle differences in their GTPase properties can lead to the accumulation of distinct intermediates in endocytic coated vesicle formation... - Evidence that dynamin-2 functions as a signal-transducing GTPaseK N Fish
Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
J Cell Biol 150:145-54. 2000..All described effects are dyn2-specific because >200-fold overexpression of dyn1, the 70% identical neuronal isoform, has no effect. Our data suggest that dyn2 can act as a signal transducing GTPase affecting transcriptional regulation... - The type III effector EspF coordinates membrane trafficking by the spatiotemporal activation of two eukaryotic signaling pathwaysNeal M Alto
Department of Pharmacology, University of California, San Diego, La Jolla, CA 92093, USA
J Cell Biol 178:1265-78. 2007..Thus, our findings suggest that the EspF-dependent assembly of SNX9 and N-WASP represents a novel form of signaling mimicry used to promote EPEC pathogenesis and gastrointestinal disease... - Robust single-particle tracking in live-cell time-lapse sequencesKhuloud Jaqaman
Department of Cell Biology, The Scripps Research Institute, 10550 N Torrey Pines Rd, La Jolla, California 92037, USA
Nat Methods 5:695-702. 2008..Both applications indicate the requirement for robust and complete tracking of dense particle fields to dissect the mechanisms of receptor organization at the level of the plasma membrane... - SNX9 couples actin assembly to phosphoinositide signals and is required for membrane remodeling during endocytosisDefne Yarar
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Dev Cell 13:43-56. 2007..These results suggest a mechanism for the spatial and temporal regulation of N-WASP-dependent actin assembly and implicate SNX9 in directly coupling actin dynamics to membrane remodeling during multiple modes of endocytosis... - SNX9 regulates dynamin assembly and is required for efficient clathrin-mediated endocytosisFabienne Soulet
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Mol Biol Cell 16:2058-67. 2005..Thus, our results establish that SNX9 is required for efficient clathrin-mediated endocytosis and suggest that it functions to regulate dynamin activity... - Identification of an adaptor-associated kinase, AAK1, as a regulator of clathrin-mediated endocytosisSean D Conner
The Scripps Research Institute, La Jolla, CA 92037, USA
J Cell Biol 156:921-9. 2002..These results also lend support to the idea that clathrin-mediated endocytosis is controlled by cycles of phosphorylation/desphosphorylation... - A dynamic actin cytoskeleton functions at multiple stages of clathrin-mediated endocytosisDefne Yarar
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Mol Biol Cell 16:964-75. 2005..Our results demonstrate a key role for actin during clathrin-mediated endocytosis in mammalian cells... - Regulated portals of entry into the cellSean D Conner
Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
Nature 422:37-44. 2003.... - An assembly-incompetent mutant establishes a requirement for dynamin self-assembly in clathrin-mediated endocytosis in vivoByeong Doo Song
Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
Mol Biol Cell 15:2243-52. 2004..Together these results provide new structural insight into the role of GED in self-assembly and assembly-stimulated GTPase activity and establish that dynamin self-assembly is essential for clathrin-mediated endocytosis... - Dynamin self-assembles into rings suggesting a mechanism for coated vesicle buddingJ E Hinshaw
Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037
Nature 374:190-2. 1995..We propose that invaginated coated pits become constricted by the assembly of dynamin into rings around their necks. A concerted conformational change would then close the rings and pinch off the budding coated vesicles... - Dynamin GTPase domain mutants that differentially affect GTP binding, GTP hydrolysis, and clathrin-mediated endocytosisByeong Doo Song
Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
J Biol Chem 279:40431-6. 2004.... - SNX9 activities are regulated by multiple phosphoinositides through both PX and BAR domainsDefne Yarar
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Traffic 9:133-46. 2008..In total, our results suggest that SNX9 can integrate signals from varied lipids through two domains to direct membrane remodeling events at multiple cellular locations... - Hsc70 is required for endocytosis and clathrin function in DrosophilaHenry C Chang
Department of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520 8002, USA
J Cell Biol 159:477-87. 2002..Together, these data provide genetic support for the long-suspected role of Hsc70 in clathrin-mediated endocytosis, at least in part by inhibiting the uncoating of CCVs... - Clathrin- and dynamin-dependent coated vesicle formation from isolated plasma membranesIshido Miwako
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Traffic 4:376-89. 2003..This assay will provide a powerful tool to dissect the minimum machinery and to probe the hierarchy of events involved in cargo selection and endocytic clathrin-coated vesicle formation... - Multivalent display and receptor-mediated endocytosis of transferrin on virus-like particlesDeboshri Banerjee
Department of Chemistry, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
Chembiochem 11:1273-9. 2010..These results suggest that cell targeting with transferrin can be improved by local concentration (avidity) effects... - Dissecting dynamin's role in clathrin-mediated endocytosisMarcel Mettlen
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Biochem Soc Trans 37:1022-6. 2009.... - Conserved functions of membrane active GTPases in coated vesicle formationThomas J Pucadyil
Department of Cell Biology, The Scripps Research Institute TSRI, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Science 325:1217-20. 2009.... - Membrane insertion of the pleckstrin homology domain variable loop 1 is critical for dynamin-catalyzed vesicle scissionRajesh Ramachandran
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Mol Biol Cell 20:4630-9. 2009..These results demonstrate that, in concert with dynamin self-assembly, PH domain membrane insertion is essential for fission and vesicle release in vitro and for clathrin-mediated endocytosis in vivo... - An intramolecular signaling element that modulates dynamin function in vitro and in vivoJoshua S Chappie
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Mol Biol Cell 20:3561-71. 2009..These data suggest that the components of the GTPase-GED interface act as an intramolecular signaling module, which we term the bundle signaling element, that can modulate dynamin function in vitro and in vivo... - Endocytic accessory proteins are functionally distinguished by their differential effects on the maturation of clathrin-coated pitsMarcel Mettlen
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Mol Biol Cell 20:3251-60. 2009.... - Cargo- and adaptor-specific mechanisms regulate clathrin-mediated endocytosisMarcel Mettlen
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
J Cell Biol 188:919-33. 2010..Altogether, our results underscore the highly dynamic and cargo-responsive nature of CCP assembly and suggest that the observed heterogeneity is, in part, related to compositional differences (e.g., cargo and adaptors) between CCPs... - Real-time visualization of dynamin-catalyzed membrane fission and vesicle releaseThomas J Pucadyil
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Cell 135:1263-75. 2008..Thus, self-limited assemblies of dynamin generated in the constant presence of GTP catalyze membrane fission... - Global and local regulation of clathrin-coated pit dynamics detected on patterned substratesAllen P Liu
Department of Cell Biology, Scripps Research Institute, La Jolla, California, USA
Biophys J 97:1038-47. 2009..Although we cannot rule out the possibility that other factors might locally affect CCP dynamics at Fn islands, our data suggest that localized modulation in cortical tension may spatially regulate clathrin-mediated endocytosis... - Isoform and splice-variant specific functions of dynamin-2 revealed by analysis of conditional knock-out cellsYa Wen Liu
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Mol Biol Cell 19:5347-59. 2008..This splice variant specificity correlated with their differential targeting to the Golgi. These studies reveal isoform and splice-variant specific functions for Dyn2... - Cargo and dynamin regulate clathrin-coated pit maturationDinah Loerke
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA, USA
PLoS Biol 7:e57. 2009..From these data, we infer the existence of an endocytic restriction or checkpoint, responsive to cargo and regulated by dynamin... - AAK1-mediated micro2 phosphorylation is stimulated by assembled clathrinSean D Conner
The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
Traffic 4:885-90. 2003..We suggest a model in which AAK1 is specifically activated in coated pits to enhance cargo recruitment and efficient internalization... - A cell-free biochemical complementation assay reveals complex and redundant cytosolic requirements for LRP endocytosisIshido Miwako
Department of Cell Biology, The Scripps Research Institute, 10550 N Torrey Pines Rd, La Jolla, CA 92037, USA
Exp Cell Res 312:1335-44. 2006..Among these, we identified Hsc70, synaptojanin1 and CRMP-2 by mass spectrometry. Our data suggest that LRP, which bears several distinct endocytic motifs in its cytoplasmic domain, may use multiple pathways for endocytosis in vitro... - Domain requirements for an endocytosis-independent, isoform-specific function of dynamin-2Fabienne Soulet
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Exp Cell Res 312:3539-45. 2006..Thus, this dynamin-2-specific function is mechanistically distinct from and independent of its role in endocytosis. These findings have important implications for interpreting dynamin-2 dependent phenotypes in overexpression studies... - The dynamin middle domain is critical for tetramerization and higher-order self-assemblyRajesh Ramachandran
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
EMBO J 26:559-66. 2007..Consequently, these mutations also impair dynamin's assembly-dependent stimulated GTPase activity... - Differential requirements for AP-2 in clathrin-mediated endocytosisSean D Conner
The Scripps Research Institute, La Jolla, CA 92037, USA
J Cell Biol 162:773-9. 2003..Surprisingly, clathrin distribution and EGF uptake were unaffected by AAK1 overexpression. Thus, AP-2 may not be stoichiometrically required for coat assembly, and may have a more cargo-selective function in CME than previously thought... - Clathrin-coated vesicle formation from isolated plasma membranesIshido Miwako
The Scripps Research Institute, Department of Cell Biology, La Jolla, California, USA
Methods Enzymol 404:503-11. 2005..Here we describe an in vitro assay for CCV formation from highly purified rat liver plasma membranes. This rapid and easy assay can be used to quantitatively evaluate the different protein requirements for different endocytic receptors... - Real-time detection reveals that effectors couple dynamin's GTP-dependent conformational changes to the membraneRajesh Ramachandran
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
EMBO J 27:27-37. 2008..Amphiphysin I has the opposite effect. Thus, dynamin's mechanochemical properties on a membrane surface are dynamically regulated by its GTPase cycle and major binding partners... - Phosphorylation of the AP2 mu subunit by AAK1 mediates high affinity binding to membrane protein sorting signalsDoris Ricotta
Institute for Biochemistry II, University of Gottingen, 37073 Gottingen, Germany
J Cell Biol 156:791-5. 2002..We propose that phosphorylation of the AP2 mu 2 subunit by AAK1 ensures high affinity binding of AP2 to sorting signals of cargo membrane proteins during the initial steps of receptor-mediated endocytosis... - Crystal structure of the GTPase domain of rat dynamin 1Thomas F Reubold
Abteilung Biophysik, Max Planck Institut fur medizinische Forschung, Jahnstrasse 29, D 69120 Heidelberg, Germany
Proc Natl Acad Sci U S A 102:13093-8. 2005..Our results support a model in which switch I residue Arg-59 gates GTP binding in an assembly-dependent manner and the GTPase effector domain functions as an assembly-dependent GTPase activating protein in the fashion of RGS-type GAPs... - An internal GAP domain negatively regulates presynaptic dynamin in vivo: a two-step model for dynamin functionRadhakrishnan Narayanan
Department of Molecular and Cellular Biology and Arizona Research Laboratories Division of Neurobiology, University of Arizona, Tucson, AZ 85721, USA
J Cell Biol 169:117-26. 2005.... - CVAK104 is a novel poly-L-lysine-stimulated kinase that targets the beta2-subunit of AP2Sean D Conner
Department of Genetics, Cell Biology, and Development, The University of Minnesota, Minneapolis, Minnesota 55455, USA
J Biol Chem 280:21539-44. 2005..CVAK104 binds ATP, and kinase assays indicate that it functions in vitro as a poly-L-lysine-stimulated kinase that is capable of autophosphorylation and phosphorylating the beta2-adaptin subunit of AP2...