S L Schmid

Summary

Affiliation: The Scripps Research Institute
Country: USA

Publications

  1. pmc Differential requirements for AP-2 in clathrin-mediated endocytosis
    Sean D Conner
    The Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Biol 162:773-9. 2003
  2. pmc Cargo- and adaptor-specific mechanisms regulate clathrin-mediated endocytosis
    Marcel Mettlen
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Biol 188:919-33. 2010
  3. pmc Identification of an adaptor-associated kinase, AAK1, as a regulator of clathrin-mediated endocytosis
    Sean D Conner
    The Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Biol 156:921-9. 2002
  4. pmc Dominant-interfering Hsc70 mutants disrupt multiple stages of the clathrin-coated vesicle cycle in vivo
    S L Newmyer
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Cell Biol 152:607-20. 2001
  5. pmc G domain dimerization controls dynamin's assembly-stimulated GTPase activity
    Joshua S Chappie
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, Maryland 20892, USA
    Nature 465:435-40. 2010
  6. pmc The Palade symposium: celebrating cell biology at its best
    Sandra L Schmid
    Department of Cell Biology, The Scripps Research Institute, University of California, San Diego, La Jolla, CA 92037, USA
    Mol Biol Cell 21:2367-70. 2010
  7. ncbi request reprint Days and knights discussing membrane dynamics in endocytosis: meeting report from the Euresco/EMBL Membrane Dynamics in Endocytosis, 6--11 October in Tomar, Portugal
    Sandra L Schmid
    The Scripps Research Institute, La Jolla, CA 92037, USA
    Traffic 3:77-85. 2002
  8. ncbi request reprint Control of EGF receptor signaling by clathrin-mediated endocytosis
    A V Vieira
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Science 274:2086-9. 1996
  9. doi request reprint Dynamin: functional design of a membrane fission catalyst
    Sandra L Schmid
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Annu Rev Cell Dev Biol 27:79-105. 2011
  10. ncbi request reprint Receptor and membrane recycling can occur with unaltered efficiency despite dramatic Rab5(q79l)-induced changes in endosome geometry
    B P Ceresa
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 276:9649-54. 2001

Collaborators

Detail Information

Publications49

  1. pmc Differential requirements for AP-2 in clathrin-mediated endocytosis
    Sean D Conner
    The Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Biol 162:773-9. 2003
    ..Surprisingly, clathrin distribution and EGF uptake were unaffected by AAK1 overexpression. Thus, AP-2 may not be stoichiometrically required for coat assembly, and may have a more cargo-selective function in CME than previously thought...
  2. pmc Cargo- and adaptor-specific mechanisms regulate clathrin-mediated endocytosis
    Marcel Mettlen
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Biol 188:919-33. 2010
    ..Altogether, our results underscore the highly dynamic and cargo-responsive nature of CCP assembly and suggest that the observed heterogeneity is, in part, related to compositional differences (e.g., cargo and adaptors) between CCPs...
  3. pmc Identification of an adaptor-associated kinase, AAK1, as a regulator of clathrin-mediated endocytosis
    Sean D Conner
    The Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Biol 156:921-9. 2002
    ..These results also lend support to the idea that clathrin-mediated endocytosis is controlled by cycles of phosphorylation/desphosphorylation...
  4. pmc Dominant-interfering Hsc70 mutants disrupt multiple stages of the clathrin-coated vesicle cycle in vivo
    S L Newmyer
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Cell Biol 152:607-20. 2001
    ..Our findings demonstrate that hsc70 indeed regulates coat disassembly and also suggest that this chaperone broadly modulates clathrin dynamics throughout the CCV cycle...
  5. pmc G domain dimerization controls dynamin's assembly-stimulated GTPase activity
    Joshua S Chappie
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, Maryland 20892, USA
    Nature 465:435-40. 2010
    ..The structure of the GTPase-GED fusion protein dimer provides insight into the mechanisms underlying dynamin-catalysed membrane fission...
  6. pmc The Palade symposium: celebrating cell biology at its best
    Sandra L Schmid
    Department of Cell Biology, The Scripps Research Institute, University of California, San Diego, La Jolla, CA 92037, USA
    Mol Biol Cell 21:2367-70. 2010
    ....
  7. ncbi request reprint Days and knights discussing membrane dynamics in endocytosis: meeting report from the Euresco/EMBL Membrane Dynamics in Endocytosis, 6--11 October in Tomar, Portugal
    Sandra L Schmid
    The Scripps Research Institute, La Jolla, CA 92037, USA
    Traffic 3:77-85. 2002
    ....
  8. ncbi request reprint Control of EGF receptor signaling by clathrin-mediated endocytosis
    A V Vieira
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Science 274:2086-9. 1996
    ..Thus, endocytic trafficking of activated EGFR plays a critical role not only in attenuating EGFR signaling but also in establishing and controlling specific signaling pathways...
  9. doi request reprint Dynamin: functional design of a membrane fission catalyst
    Sandra L Schmid
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Annu Rev Cell Dev Biol 27:79-105. 2011
    ....
  10. ncbi request reprint Receptor and membrane recycling can occur with unaltered efficiency despite dramatic Rab5(q79l)-induced changes in endosome geometry
    B P Ceresa
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 276:9649-54. 2001
    ..These results suggest that GTP hydrolysis by Rab5 is rate-limiting for endosome fusion but not for endocytic trafficking and that early endosome geometry may be a less critical determinant of sorting efficiencies than previously thought...
  11. pmc Dynamin GTPase domain mutants block endocytic vesicle formation at morphologically distinct stages
    H Damke
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Mol Biol Cell 12:2578-89. 2001
    ..Nonetheless, we find that overexpression of dynamin mutants with subtle differences in their GTPase properties can lead to the accumulation of distinct intermediates in endocytic coated vesicle formation...
  12. pmc Clathrin-independent pinocytosis is induced in cells overexpressing a temperature-sensitive mutant of dynamin
    H Damke
    Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037, USA
    J Cell Biol 131:69-80. 1995
    ....
  13. ncbi request reprint Dynamin GTPase, a force-generating molecular switch
    D E Warnock
    Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92037, USA
    Bioessays 18:885-93. 1996
    ..In this case, dynamin would be the first GTPase identified that acts as a structural protein with mechano-chemical function...
  14. pmc Evidence that dynamin-2 functions as a signal-transducing GTPase
    K N Fish
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Cell Biol 150:145-54. 2000
    ..Our data suggest that dyn2 can act as a signal transducing GTPase affecting transcriptional regulation...
  15. pmc The type III effector EspF coordinates membrane trafficking by the spatiotemporal activation of two eukaryotic signaling pathways
    Neal M Alto
    Department of Pharmacology, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 178:1265-78. 2007
    ..Thus, our findings suggest that the EspF-dependent assembly of SNX9 and N-WASP represents a novel form of signaling mimicry used to promote EPEC pathogenesis and gastrointestinal disease...
  16. pmc Robust single-particle tracking in live-cell time-lapse sequences
    Khuloud Jaqaman
    Department of Cell Biology, The Scripps Research Institute, 10550 N Torrey Pines Rd, La Jolla, California 92037, USA
    Nat Methods 5:695-702. 2008
    ..Both applications indicate the requirement for robust and complete tracking of dense particle fields to dissect the mechanisms of receptor organization at the level of the plasma membrane...
  17. ncbi request reprint SNX9 couples actin assembly to phosphoinositide signals and is required for membrane remodeling during endocytosis
    Defne Yarar
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Dev Cell 13:43-56. 2007
    ..These results suggest a mechanism for the spatial and temporal regulation of N-WASP-dependent actin assembly and implicate SNX9 in directly coupling actin dynamics to membrane remodeling during multiple modes of endocytosis...
  18. pmc SNX9 regulates dynamin assembly and is required for efficient clathrin-mediated endocytosis
    Fabienne Soulet
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Biol Cell 16:2058-67. 2005
    ..Thus, our results establish that SNX9 is required for efficient clathrin-mediated endocytosis and suggest that it functions to regulate dynamin activity...
  19. pmc A dynamic actin cytoskeleton functions at multiple stages of clathrin-mediated endocytosis
    Defne Yarar
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Biol Cell 16:964-75. 2005
    ..Our results demonstrate a key role for actin during clathrin-mediated endocytosis in mammalian cells...
  20. pmc An assembly-incompetent mutant establishes a requirement for dynamin self-assembly in clathrin-mediated endocytosis in vivo
    Byeong Doo Song
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Mol Biol Cell 15:2243-52. 2004
    ..Together these results provide new structural insight into the role of GED in self-assembly and assembly-stimulated GTPase activity and establish that dynamin self-assembly is essential for clathrin-mediated endocytosis...
  21. ncbi request reprint Regulated portals of entry into the cell
    Sean D Conner
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Nature 422:37-44. 2003
    ....
  22. ncbi request reprint Dynamin self-assembles into rings suggesting a mechanism for coated vesicle budding
    J E Hinshaw
    Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037
    Nature 374:190-2. 1995
    ..We propose that invaginated coated pits become constricted by the assembly of dynamin into rings around their necks. A concerted conformational change would then close the rings and pinch off the budding coated vesicles...
  23. ncbi request reprint SNX9 activities are regulated by multiple phosphoinositides through both PX and BAR domains
    Defne Yarar
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Traffic 9:133-46. 2008
    ..In total, our results suggest that SNX9 can integrate signals from varied lipids through two domains to direct membrane remodeling events at multiple cellular locations...
  24. pmc Hsc70 is required for endocytosis and clathrin function in Drosophila
    Henry C Chang
    Department of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520 8002, USA
    J Cell Biol 159:477-87. 2002
    ..Together, these data provide genetic support for the long-suspected role of Hsc70 in clathrin-mediated endocytosis, at least in part by inhibiting the uncoating of CCVs...
  25. ncbi request reprint Clathrin- and dynamin-dependent coated vesicle formation from isolated plasma membranes
    Ishido Miwako
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Traffic 4:376-89. 2003
    ..This assay will provide a powerful tool to dissect the minimum machinery and to probe the hierarchy of events involved in cargo selection and endocytic clathrin-coated vesicle formation...
  26. ncbi request reprint Multivalent display and receptor-mediated endocytosis of transferrin on virus-like particles
    Deboshri Banerjee
    Department of Chemistry, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Chembiochem 11:1273-9. 2010
    ..These results suggest that cell targeting with transferrin can be improved by local concentration (avidity) effects...
  27. ncbi request reprint Dynamin GTPase domain mutants that differentially affect GTP binding, GTP hydrolysis, and clathrin-mediated endocytosis
    Byeong Doo Song
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 279:40431-6. 2004
    ....
  28. pmc The dynamin middle domain is critical for tetramerization and higher-order self-assembly
    Rajesh Ramachandran
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    EMBO J 26:559-66. 2007
    ..Consequently, these mutations also impair dynamin's assembly-dependent stimulated GTPase activity...
  29. ncbi request reprint AAK1-mediated micro2 phosphorylation is stimulated by assembled clathrin
    Sean D Conner
    The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Traffic 4:885-90. 2003
    ..We suggest a model in which AAK1 is specifically activated in coated pits to enhance cargo recruitment and efficient internalization...
  30. pmc Membrane insertion of the pleckstrin homology domain variable loop 1 is critical for dynamin-catalyzed vesicle scission
    Rajesh Ramachandran
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Biol Cell 20:4630-9. 2009
    ..These results demonstrate that, in concert with dynamin self-assembly, PH domain membrane insertion is essential for fission and vesicle release in vitro and for clathrin-mediated endocytosis in vivo...
  31. pmc Dissecting dynamin's role in clathrin-mediated endocytosis
    Marcel Mettlen
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Biochem Soc Trans 37:1022-6. 2009
    ....
  32. pmc Conserved functions of membrane active GTPases in coated vesicle formation
    Thomas J Pucadyil
    Department of Cell Biology, The Scripps Research Institute TSRI, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 325:1217-20. 2009
    ....
  33. pmc Global and local regulation of clathrin-coated pit dynamics detected on patterned substrates
    Allen P Liu
    Department of Cell Biology, Scripps Research Institute, La Jolla, California, USA
    Biophys J 97:1038-47. 2009
    ..Although we cannot rule out the possibility that other factors might locally affect CCP dynamics at Fn islands, our data suggest that localized modulation in cortical tension may spatially regulate clathrin-mediated endocytosis...
  34. pmc An intramolecular signaling element that modulates dynamin function in vitro and in vivo
    Joshua S Chappie
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Biol Cell 20:3561-71. 2009
    ..These data suggest that the components of the GTPase-GED interface act as an intramolecular signaling module, which we term the bundle signaling element, that can modulate dynamin function in vitro and in vivo...
  35. ncbi request reprint A cell-free biochemical complementation assay reveals complex and redundant cytosolic requirements for LRP endocytosis
    Ishido Miwako
    Department of Cell Biology, The Scripps Research Institute, 10550 N Torrey Pines Rd, La Jolla, CA 92037, USA
    Exp Cell Res 312:1335-44. 2006
    ..Among these, we identified Hsc70, synaptojanin1 and CRMP-2 by mass spectrometry. Our data suggest that LRP, which bears several distinct endocytic motifs in its cytoplasmic domain, may use multiple pathways for endocytosis in vitro...
  36. ncbi request reprint Clathrin-coated vesicle formation from isolated plasma membranes
    Ishido Miwako
    The Scripps Research Institute, Department of Cell Biology, La Jolla, California, USA
    Methods Enzymol 404:503-11. 2005
    ..Here we describe an in vitro assay for CCV formation from highly purified rat liver plasma membranes. This rapid and easy assay can be used to quantitatively evaluate the different protein requirements for different endocytic receptors...
  37. pmc Endocytic accessory proteins are functionally distinguished by their differential effects on the maturation of clathrin-coated pits
    Marcel Mettlen
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Biol Cell 20:3251-60. 2009
    ....
  38. ncbi request reprint Domain requirements for an endocytosis-independent, isoform-specific function of dynamin-2
    Fabienne Soulet
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Exp Cell Res 312:3539-45. 2006
    ..Thus, this dynamin-2-specific function is mechanistically distinct from and independent of its role in endocytosis. These findings have important implications for interpreting dynamin-2 dependent phenotypes in overexpression studies...
  39. pmc Isoform and splice-variant specific functions of dynamin-2 revealed by analysis of conditional knock-out cells
    Ya Wen Liu
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Biol Cell 19:5347-59. 2008
    ..This splice variant specificity correlated with their differential targeting to the Golgi. These studies reveal isoform and splice-variant specific functions for Dyn2...
  40. pmc Real-time visualization of dynamin-catalyzed membrane fission and vesicle release
    Thomas J Pucadyil
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Cell 135:1263-75. 2008
    ..Thus, self-limited assemblies of dynamin generated in the constant presence of GTP catalyze membrane fission...
  41. pmc Cargo and dynamin regulate clathrin-coated pit maturation
    Dinah Loerke
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA, USA
    PLoS Biol 7:e57. 2009
    ..From these data, we infer the existence of an endocytic restriction or checkpoint, responsive to cargo and regulated by dynamin...
  42. pmc Real-time detection reveals that effectors couple dynamin's GTP-dependent conformational changes to the membrane
    Rajesh Ramachandran
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    EMBO J 27:27-37. 2008
    ..Amphiphysin I has the opposite effect. Thus, dynamin's mechanochemical properties on a membrane surface are dynamically regulated by its GTPase cycle and major binding partners...
  43. pmc Phosphorylation of the AP2 mu subunit by AAK1 mediates high affinity binding to membrane protein sorting signals
    Doris Ricotta
    Institute for Biochemistry II, University of Gottingen, 37073 Gottingen, Germany
    J Cell Biol 156:791-5. 2002
    ..We propose that phosphorylation of the AP2 mu 2 subunit by AAK1 ensures high affinity binding of AP2 to sorting signals of cargo membrane proteins during the initial steps of receptor-mediated endocytosis...
  44. pmc Crystal structure of the GTPase domain of rat dynamin 1
    Thomas F Reubold
    Abteilung Biophysik, Max Planck Institut fur medizinische Forschung, Jahnstrasse 29, D 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 102:13093-8. 2005
    ..Our results support a model in which switch I residue Arg-59 gates GTP binding in an assembly-dependent manner and the GTPase effector domain functions as an assembly-dependent GTPase activating protein in the fashion of RGS-type GAPs...
  45. pmc An internal GAP domain negatively regulates presynaptic dynamin in vivo: a two-step model for dynamin function
    Radhakrishnan Narayanan
    Department of Molecular and Cellular Biology and Arizona Research Laboratories Division of Neurobiology, University of Arizona, Tucson, AZ 85721, USA
    J Cell Biol 169:117-26. 2005
    ....
  46. ncbi request reprint CVAK104 is a novel poly-L-lysine-stimulated kinase that targets the beta2-subunit of AP2
    Sean D Conner
    Department of Genetics, Cell Biology, and Development, The University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Biol Chem 280:21539-44. 2005
    ..CVAK104 binds ATP, and kinase assays indicate that it functions in vitro as a poly-L-lysine-stimulated kinase that is capable of autophosphorylation and phosphorylating the beta2-adaptin subunit of AP2...