S Sastry

Summary

Affiliation: The Rockefeller University
Country: USA

Publications

  1. ncbi Identification of the template-binding cleft of T7 RNA polymerase as the site for promoter binding by photochemical cross-linking with psoralen
    S S Sastry
    Laboratory of Molecular Genetics, Rockefeller University, New York, New York 10021, USA
    Biochemistry 35:13519-30. 1996
  2. ncbi Probing the interaction of T7 RNA polymerase with promoter
    S Sastry
    Laboratory of Molecular Genetics, The Rockefeller University, New York 10021, USA
    Biochemistry 38:4972-81. 1999
  3. ncbi Molecular mechanisms of peptide loading by the tumor rejection antigen/heat shock chaperone gp96 (GRP94)
    S Sastry
    Laboratory of Molecular Genetics, The Rockefeller University, New York, New York 10021, USA
    J Biol Chem 274:12023-35. 1999
  4. ncbi RNA-binding site in T7 RNA polymerase
    S Sastry
    Laboratory of Molecular Genetics, Box 174, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 95:9111-6. 1998
  5. ncbi Mechanisms for the processing of a frozen topoisomerase-DNA conjugate by human cell-free extracts
    S Sastry
    Laboratory of Molecular Genetics, The Rockefeller University, New York, New York 10021, USA
    J Biol Chem 273:9942-50. 1998
  6. ncbi Isolation and partial characterization of a novel psoralen-tyrosine photoconjugate from a photoreaction of psoralen with a natural protein
    S S Sastry
    Laboratory of Molecular Genetics, Rockefeller University, New York, NY 10021, USA
    Photochem Photobiol 65:937-44. 1997
  7. ncbi Probing the mechanisms of T7 RNA polymerase transcription initiation using photochemical conjugation of psoralen to a promoter
    S S Sastry
    Laboratory of Molecular Genetics, Rockefeller University, New York, New York 10021, USA
    Biochemistry 36:3133-44. 1997
  8. ncbi Cross-linking of DNA-binding proteins to DNA with psoralen and psoralen furan-side monoadducts. Comparison of action spectra with DNA-DNA cross-linking
    S S Sastry
    Laboratory of Molecular Genetics, and the Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, USA
    J Biol Chem 272:3715-23. 1997
  9. ncbi A direct real-time spectroscopic investigation of the mechanism of open complex formation by T7 RNA polymerase
    S S Sastry
    Laboratory of Molecular Genetics, Rockefeller University, New York, New York 10021, USA
    Biochemistry 35:15715-25. 1996
  10. ncbi A fluorescence-based assay for transcription using a novel fluorescent GTP analogue
    S Sastry
    Laboratory of Molecular Genetics, Box 174, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Biophys Chem 91:191-208. 2001

Collaborators

  • N A Linderoth
  • M N Simon
  • J F Hainfeld

Detail Information

Publications11

  1. ncbi Identification of the template-binding cleft of T7 RNA polymerase as the site for promoter binding by photochemical cross-linking with psoralen
    S S Sastry
    Laboratory of Molecular Genetics, Rockefeller University, New York, New York 10021, USA
    Biochemistry 35:13519-30. 1996
    ..The photochemical techniques we have developed [Sastry et al. (1993) Biochemistry 32, 5526-5538; this work] can be applied to other DNA-protein complexes to map DNA-binding domains...
  2. ncbi Probing the interaction of T7 RNA polymerase with promoter
    S Sastry
    Laboratory of Molecular Genetics, The Rockefeller University, New York 10021, USA
    Biochemistry 38:4972-81. 1999
    ..High-resolution mapping indicated that amino acid residues 614-627 may be in the vicinity of the melting domain. Specifically, Y623 may contact -3 on the template strand...
  3. ncbi Molecular mechanisms of peptide loading by the tumor rejection antigen/heat shock chaperone gp96 (GRP94)
    S Sastry
    Laboratory of Molecular Genetics, The Rockefeller University, New York, New York 10021, USA
    J Biol Chem 274:12023-35. 1999
    ..It is proposed that peptides are assembled within higher order gp96 complexes (dimers, etc.) in a hydrophobic pocket and that there may be a conformational change in gp96 leading to an open configuration for peptide loading...
  4. ncbi RNA-binding site in T7 RNA polymerase
    S Sastry
    Laboratory of Molecular Genetics, Box 174, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 95:9111-6. 1998
    ....
  5. ncbi Mechanisms for the processing of a frozen topoisomerase-DNA conjugate by human cell-free extracts
    S Sastry
    Laboratory of Molecular Genetics, The Rockefeller University, New York, New York 10021, USA
    J Biol Chem 273:9942-50. 1998
    ..These processes may represent the "repair" of DNA-protein cross-links...
  6. ncbi Isolation and partial characterization of a novel psoralen-tyrosine photoconjugate from a photoreaction of psoralen with a natural protein
    S S Sastry
    Laboratory of Molecular Genetics, Rockefeller University, New York, NY 10021, USA
    Photochem Photobiol 65:937-44. 1997
    ..As far as we know, this work represents the first instance of isolation and partial characterization of an amino acid-psoralen photoadduct...
  7. ncbi Probing the mechanisms of T7 RNA polymerase transcription initiation using photochemical conjugation of psoralen to a promoter
    S S Sastry
    Laboratory of Molecular Genetics, Rockefeller University, New York, New York 10021, USA
    Biochemistry 36:3133-44. 1997
    ....
  8. ncbi Cross-linking of DNA-binding proteins to DNA with psoralen and psoralen furan-side monoadducts. Comparison of action spectra with DNA-DNA cross-linking
    S S Sastry
    Laboratory of Molecular Genetics, and the Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, USA
    J Biol Chem 272:3715-23. 1997
    ..Based on the action spectra and the known excited states of psoralen, it is suggested that the triplet n,pi* transition localized in the C-2=O of psoralen may be involved in protein-psoralen photoreactions...
  9. ncbi A direct real-time spectroscopic investigation of the mechanism of open complex formation by T7 RNA polymerase
    S S Sastry
    Laboratory of Molecular Genetics, Rockefeller University, New York, New York 10021, USA
    Biochemistry 35:15715-25. 1996
    ....
  10. ncbi A fluorescence-based assay for transcription using a novel fluorescent GTP analogue
    S Sastry
    Laboratory of Molecular Genetics, Box 174, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Biophys Chem 91:191-208. 2001
    ..The results suggested that G-ladder RNAs adapt unusual conformations such as G-quartets. Thus, the new fluorescent probe can be useful for structural studies on RNA...
  11. ncbi Binding of antigenic peptide to the endoplasmic reticulum-resident protein gp96/GRP94 heat shock chaperone occurs in higher order complexes. Essential role of some aromatic amino acid residues in the peptide-binding site
    N A Linderoth
    Laboratory of Molecular Genetics, The Rockefeller University, New York, New York 10021 and the Department of Biology, Brookhaven National Laboratory, Upton, New York 11973
    J Biol Chem 276:11049-54. 2001
    ..Probing the peptide microenvironment in gp96-peptide complexes, suggested that hydrophobic interactions (and perhaps hydrogen bonding/stacking interactions) may play a role in peptide loading by gp96...