Thomas P Sakmar


Affiliation: The Rockefeller University
Country: USA


  1. Tian H, Sakmar T, Huber T. A simple method for enhancing the bioorthogonality of cyclooctyne reagent. Chem Commun (Camb). 2016;52:5451-4 pubmed publisher
    ..We site-specifically label a genetically-encoded azido group in the visual photoreceptor rhodopsin to demonstrate the utility of the strategy. ..
  2. Graham W, Bonito Oliva A, Sakmar T. Update on Alzheimer's Disease Therapy and Prevention Strategies. Annu Rev Med. 2017;68:413-430 pubmed publisher
    ..The main focus of this review is to summarize the most recent basic biology, biochemistry, and pharmacology that serve as a foundation for more than 50 active advanced-phase clinical trials for AD prevention and therapy. ..
  3. Lorenzen E, Ceraudo E, Berchiche Y, Rico C, Fürstenberg A, Sakmar T, et al. G protein subtype-specific signaling bias in a series of CCR5 chemokine analogs. Sci Signal. 2018;11: pubmed publisher
    ..We propose that G protein subtype-specific signaling bias may be a general feature of GPCRs that can couple to more than one G protein family. ..
  4. Lorenzen E, Sakmar T. Receptor Structures for a Caldron of Cannabinoids. Cell. 2019;176:409-411 pubmed publisher
    ..2019) and CB2 in complex with a new rationally designed inverse agonist (Li et al., 2019) provide unique snapshots of the molecular pharmacology of cannabinoids. ..
  5. Barbash S, Persson T, Lorenzen E, Kazmi M, Huber T, Sakmar T. Detection of Concordance between Transcriptional Levels of GPCRs and Receptor-Activity-Modifying Proteins. iScience. 2019;11:366-374 pubmed publisher
    ..These results provide additional evidence that GPCR-RAMP interactions are more widespread than previously appreciated and that these interactions have functional consequences. ..
  6. Huber T, Sakmar T. Chemical biology methods for investigating G protein-coupled receptor signaling. Chem Biol. 2014;21:1224-37 pubmed publisher
  7. Huber T, Sakmar T. Probing Antibody Binding Sites on G Protein-Coupled Receptors Using Genetically Encoded Photo-Activatable Cross-Linkers. Methods Mol Biol. 2018;1785:65-75 pubmed publisher
    ..The targeted photo-cross-linking method is complementary to loss-of-function mutagenesis and is especially useful to study anti-receptor antibodies with discontinuous epitopes. ..
  8. Tian H, Sakmar T, Huber T. Micelle-Enhanced Bioorthogonal Labeling of Genetically Encoded Azido Groups on the Lipid-Embedded Surface of a GPCR. Chembiochem. 2015;16:1314-22 pubmed publisher
    ..Targeting hydrophobic regions of membrane proteins by use of micelle-enhanced SpAAC should expand the utility of bioorthogonal labeling strategies. ..
  9. Park M, Sivertsen B, Els Heindl S, Huber T, Holst B, Beck Sickinger A, et al. Bioorthogonal Labeling of Ghrelin Receptor to Facilitate Studies of Ligand-Dependent Conformational Dynamics. Chem Biol. 2015;22:1431-1436 pubmed publisher
    ..We demonstrate how conformational dynamics of a G-protein-coupled receptor can be measured in reconstituted systems. ..

More Information


  1. Berchiche Y, Sakmar T. CXC Chemokine Receptor 3 Alternative Splice Variants Selectively Activate Different Signaling Pathways. Mol Pharmacol. 2016;90:483-95 pubmed publisher
    ..Our data show an additional layer of complexity for chemokine receptor signaling that might be exploited to target specific CXCR3 splice variants. ..
  2. Tian H, Sakmar T, Huber T. Measurement of Slow Spontaneous Release of 11-cis-Retinal from Rhodopsin. Biophys J. 2017;112:153-161 pubmed publisher
    ..We also discuss the physiological implications of these findings on the basal activity of opsins and the associated dark noise in the visual system. ..
  3. Barbash S, Sakmar T. Length-dependent gene misexpression is associated with Alzheimer's disease progression. Sci Rep. 2017;7:190 pubmed publisher
    ..Additional investigation to elucidate the precise mechanism underlying gene-length dependent changes in expression is warranted. ..
  4. Tian H, Sakmar T, Huber T. The Energetics of Chromophore Binding in the Visual Photoreceptor Rhodopsin. Biophys J. 2017;113:60-72 pubmed publisher
    ..Our data provide insights into the ligand-receptor binding reaction for rhodopsin in particular, and for GPCRs more broadly. ..
  5. Peeler J, Schedin Weiss S, Soula M, Kazmi M, Sakmar T. Isopeptide and ester bond ubiquitination both regulate degradation of the human dopamine receptor 4. J Biol Chem. 2017;292:21623-21630 pubmed publisher
    ..We conclude that both isopeptide and ester bond ubiquitination regulate proteasomal degradation of hD4R. ..
  6. Barbash S, Sakmar T. Brain gene expression signature on primate genomic sequence evolution. Sci Rep. 2017;7:17329 pubmed publisher
    ..The similarity was specific for promoter regions and cerebellum and frontal cortex expression, suggesting a major impact of gene regulation in the brain on genome shaping along the primate branch. ..