Wolfram Ruf

Summary

Affiliation: The Scripps Research Institute
Country: USA

Publications

  1. ncbi request reprint Role of the protein C receptor in cancer progression
    Wolfram Ruf
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA, USA Center for Thrombosis and Hemostasis, Universitatsmedizin, Johannes Gutenberg University, Mainz, Germany Electronic address
    Thromb Res 133:S85-9. 2014
  2. pmc Science review: role of coagulation protease cascades in sepsis
    Matthias Riewald
    Department of Immunology C204, The Scripps Research Institute, La Jolla, California, USA
    Crit Care 7:123-9. 2003
  3. pmc Tissue factor-protease-activated receptor 2 signaling promotes diet-induced obesity and adipose inflammation
    Leylla Badeanlou
    Department of Cell Biology, Torrey Pines Institute for Molecular Studies, San Diego, California, USA
    Nat Med 17:1490-7. 2011
  4. pmc Tissue factor and cancer
    Wolfram Ruf
    Department of Immunology and Microbial Science, SP258, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, United States
    Thromb Res 130:S84-7. 2012
  5. pmc Role of thiol pathways in TF procoagulant regulation
    Wolfram Ruf
    Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Thromb Res 129:S11-2. 2012
  6. ncbi request reprint Specificity of coagulation factor signaling
    W Ruf
    Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Thromb Haemost 1:1495-503. 2003
  7. ncbi request reprint Tissue factor-dependent signaling in tumor biology
    Wolfram Ruf
    Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Pathophysiol Haemost Thromb 33:28-30. 2003
  8. ncbi request reprint Differential role of tissue factor pathway inhibitors 1 and 2 in melanoma vasculogenic mimicry
    Wolfram Ruf
    Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
    Cancer Res 63:5381-9. 2003
  9. ncbi request reprint Emerging roles of tissue factor in viral hemorrhagic fever
    Wolfram Ruf
    Department of Immunology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Trends Immunol 25:461-4. 2004
  10. pmc Vascular and dendritic cell coagulation signaling in sepsis progression
    W Ruf
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Thromb Haemost 7:118-21. 2009

Detail Information

Publications79

  1. ncbi request reprint Role of the protein C receptor in cancer progression
    Wolfram Ruf
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA, USA Center for Thrombosis and Hemostasis, Universitatsmedizin, Johannes Gutenberg University, Mainz, Germany Electronic address
    Thromb Res 133:S85-9. 2014
    ....
  2. pmc Science review: role of coagulation protease cascades in sepsis
    Matthias Riewald
    Department of Immunology C204, The Scripps Research Institute, La Jolla, California, USA
    Crit Care 7:123-9. 2003
    ..This dichotomy is of relevance for the interpretation of preclinical and clinical data that document nonuniform responses to anticoagulant strategies in sepsis therapy...
  3. pmc Tissue factor-protease-activated receptor 2 signaling promotes diet-induced obesity and adipose inflammation
    Leylla Badeanlou
    Department of Cell Biology, Torrey Pines Institute for Molecular Studies, San Diego, California, USA
    Nat Med 17:1490-7. 2011
    ..Thus, inhibition of tissue factor signaling is a potential therapeutic avenue for improving impaired metabolism and insulin resistance in obesity...
  4. pmc Tissue factor and cancer
    Wolfram Ruf
    Department of Immunology and Microbial Science, SP258, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, United States
    Thromb Res 130:S84-7. 2012
    ..Basic research continues to identify pivotal molecular interactions in these processes and provides potential leads for targeting specific tumor promoting pathways associated with hemostasis and thrombosis...
  5. pmc Role of thiol pathways in TF procoagulant regulation
    Wolfram Ruf
    Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Thromb Res 129:S11-2. 2012
    ..Genetic deletion of P2X7 signaling attenuates FeCl(3)-induced carotid artery thrombosis in mice, indicating that TF prothrombotic activity is regulated by specific cell signaling pathways in vivo...
  6. ncbi request reprint Specificity of coagulation factor signaling
    W Ruf
    Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Thromb Haemost 1:1495-503. 2003
    ..Protease receptors are thus major determinants of the biological outcome of coagulation factor signaling on vascular cells...
  7. ncbi request reprint Tissue factor-dependent signaling in tumor biology
    Wolfram Ruf
    Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Pathophysiol Haemost Thromb 33:28-30. 2003
  8. ncbi request reprint Differential role of tissue factor pathway inhibitors 1 and 2 in melanoma vasculogenic mimicry
    Wolfram Ruf
    Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
    Cancer Res 63:5381-9. 2003
    ..Thus, TFPI-2 contributes to VM plasticity, whereas TFPI-1 has anticoagulant functions of relevance for perfusion of VM channels formed by TF-expressing melanoma cells...
  9. ncbi request reprint Emerging roles of tissue factor in viral hemorrhagic fever
    Wolfram Ruf
    Department of Immunology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Trends Immunol 25:461-4. 2004
    ..Thus, targeting TF appears to aid the antiviral immune response in hemorrhagic fevers, and further studies are encouraged to define how TF-dependent signaling regulates immunity...
  10. pmc Vascular and dendritic cell coagulation signaling in sepsis progression
    W Ruf
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Thromb Haemost 7:118-21. 2009
    ....
  11. ncbi request reprint Is APC activation of endothelial cell PAR1 important in severe sepsis?: Yes
    W Ruf
    Department of Immunology, SP258, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Thromb Haemost 3:1912-4. 2005
  12. pmc New players in the sepsis-protective activated protein C pathway
    Wolfram Ruf
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California 92037, USA
    J Clin Invest 120:3084-7. 2010
    ....
  13. ncbi request reprint Redundant signaling of tissue factor and thrombin in cancer progression?
    W Ruf
    Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Thromb Haemost 5:1584-7. 2007
  14. pmc Tissue factor in cancer progression and angiogenesis
    Wolfram Ruf
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA
    Thromb Res 125:S36-8. 2010
    ..Thus, interruption of TF signaling represents a potential anti-angiogenic strategy that does not carry an increased risk of bleeding associated with prolonged inhibition of the TF coagulation pathway...
  15. ncbi request reprint Tissue factor and PAR signaling in tumor progression
    Wolfram Ruf
    Department of Immunology, Scripps Research Institute, La Jolla, CA, USA
    Thromb Res 120:S7-12. 2007
    ..A combination of experimental models of spontaneous and transplanted tumor development and metastasis start to delineate the role of TF in tumor progression and identified potential therapeutic approaches to target the TF pathway...
  16. ncbi request reprint Molecular regulation of blood clotting in tumor biology
    W Ruf
    Department of Immunology and Vascular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Haemostasis 31:5-7. 2001
    ..e. TF-VIIa-Xa, generates autocrine cell signaling though protease activated receptors. A cooperation of the TF cytoplasmic domain with protease signaling may explain the diverse contributions of TF to metastasis and angiogenesis...
  17. ncbi request reprint Diverse functions of protease receptor tissue factor in inflammation and metastasis
    W Ruf
    Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Immunol Res 21:289-92. 2000
    ....
  18. ncbi request reprint The interaction of activated factor VII with tissue factor: insight into the mechanism of cofactor-mediated activation of activated factor VII
    W Ruf
    Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Blood Coagul Fibrinolysis 9:S73-8. 1998
    ..Combining these findings with the results of mutational analysis of TF, a picture emerges whereby multiple domains of both cofactor and enzyme provide contacts for the assembly of macromolecular substrate...
  19. pmc Endogenous EPCR/aPC-PAR1 signaling prevents inflammation-induced vascular leakage and lethality
    Frank Niessen
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA
    Blood 113:2859-66. 2009
    ..Thus, the activating proteases and selectivity in coupling to S1P receptor subtypes determine vascular PAR1 signaling specificity in systemic inflammatory response syndromes in vivo...
  20. pmc The endothelial protein C receptor supports tissue factor ternary coagulation initiation complex signaling through protease-activated receptors
    Jennifer Disse
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 286:5756-67. 2011
    ....
  21. ncbi request reprint Protease-activated receptor-1 signaling by activated protein C in cytokine-perturbed endothelial cells is distinct from thrombin signaling
    Matthias Riewald
    Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 280:19808-14. 2005
    ..These data may explain how APC can be therapeutically protective through the EPCR-PAR1 signaling despite ongoing thrombin generation due to disseminated intravascular coagulopathy...
  22. pmc Inhibition of tissue factor signaling suppresses tumor growth
    Henri H Versteeg
    Department of Immunology, SP258, Scripps Research Institute, La Jolla, CA 92037, USA
    Blood 111:190-9. 2008
    ..These results show that tumor cell TF-PAR2 signaling is crucial for tumor growth and suggest that anti-TF strategies can be applied in cancer therapy with minor impairment of TF-dependent hemostatic pathways...
  23. pmc Factor Xa binding to annexin 2 mediates signal transduction via protease-activated receptor 1
    Gourab Bhattacharjee
    Scripps Research Institute, SP 258, 10550 N Torrey Pines Rd, La Jolla, CA 92037, USA
    Circ Res 102:457-64. 2008
    ..We propose that annexin 2 serves to regulate factor Xa signaling specifically in the absence of cell surface TF and may thus play physiological or pathological roles when factor Xa is generated in a TF-depleted environment...
  24. pmc Organ-specific protection against lipopolysaccharide-induced vascular leak is dependent on the endothelial protein C receptor
    Annette Von Drygalski
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, MEM 180, 10550 North Torrey Pines Rd, La Jolla, CA 92037, USA
    Arterioscler Thromb Vasc Biol 33:769-76. 2013
    ..To study the role of the endothelial protein C receptor (EPCR) in the modulation of susceptibility to inflammation-induced vascular leak in vivo...
  25. ncbi request reprint Role of protease activated receptor 1 and 2 signaling in hypoxia-induced angiogenesis
    Hannele Uusitalo-Jarvinen
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Arterioscler Thromb Vasc Biol 27:1456-62. 2007
    ..The TF-VIIa complex also directly cleaves PAR2 and promotes angiogenesis in vitro in TF cytoplasmic domain-deleted (TF(deltaCT)) mice. Here we address the effect of PAR1 and PAR2 deficiency on angiogenesis in vivo...
  26. doi request reprint Dendritic cell PAR1-S1P3 signalling couples coagulation and inflammation
    Frank Niessen
    Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
    Nature 452:654-8. 2008
    ..Thus, activation of dendritic cells by coagulation in the lymphatics emerges as a previously unknown mechanism that promotes systemic inflammation and lethality in decompensated innate immune responses...
  27. ncbi request reprint Regulation of tissue factor-induced signaling by endogenous and recombinant tissue factor pathway inhibitor 1
    Jasimuddin Ahamed
    Department of Immunology, SP258, The Scripps Research Institute, 10550 North Torrey Pines Rd, La Jolla, CA 92037, USA
    Blood 105:2384-91. 2005
    ....
  28. pmc Molecular dynamics simulations and functional characterization of the interactions of the PAR2 ectodomain with factor VIIa
    Qing Zhang
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Proteins 77:559-69. 2009
    ....
  29. ncbi request reprint Orchestration of coagulation protease signaling by tissue factor
    Matthias Riewald
    Department of Immunology C204, The Scripps Research Institute, La Jolla, CA 92037, USA
    Trends Cardiovasc Med 12:149-54. 2002
    ..Targeting inflammatory cell signaling events of the coagulation system may become an important aspect of efforts to improve antithrombotic therapy...
  30. pmc Protease-activated receptor (PAR) 2, but not PAR1, signaling promotes the development of mammary adenocarcinoma in polyoma middle T mice
    Henri H Versteeg
    Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Cancer Res 68:7219-27. 2008
    ..This study establishes that protease signaling contributes to mammary tumor development and that PAR2, rather than the thrombin receptor PAR1, plays a crucial role in the angiogenic switch...
  31. pmc Endothelial protein C receptor function in murine and human breast cancer development
    Florence Schaffner
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California, United States of America
    PLoS ONE 8:e61071. 2013
    ....
  32. ncbi request reprint Tissue factor coagulant function is enhanced by protein-disulfide isomerase independent of oxidoreductase activity
    Henri H Versteeg
    Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 282:25416-24. 2007
    ..PDI enhanced TF coagulant activity on microvesicles shed from cells, suggesting that PDI plays a role as an activating chaperone for circulating cryptic TF...
  33. ncbi request reprint Activation of endothelial cell protease activated receptor 1 by the protein C pathway
    Matthias Riewald
    Department of Immunology, C204, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 296:1880-2. 2002
    ..Thus, the prototypical thrombin receptor is the target for EPCR-dependent APC signaling, suggesting a role for this receptor cascade in protection from sepsis...
  34. ncbi request reprint Emerging insights in tissue factor-dependent signaling events
    Henri H Versteeg
    Department of Immunology, The Scripps Institute, La Jolla, CA 92037, USA
    Semin Thromb Hemost 32:24-32. 2006
    ..In this review, we relate our current understanding of direct TF signaling pathways to the emerging roles of TF in (patho)physiology...
  35. doi request reprint Tissue factor and protease-activated receptor signaling in cancer
    Florence Schaffner
    Research Associate, Department of Immunology, The Scripps Research Institute, La Jolla, California, USA
    Semin Thromb Hemost 34:147-53. 2008
    ....
  36. ncbi request reprint Activated protein C signals through the thrombin receptor PAR1 in endothelial cells
    Matthias Riewald
    Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Endotoxin Res 9:317-21. 2003
    ..These data demonstrate unexpected protective functions of the major thrombin receptor PAR1 in endothelial cells...
  37. pmc Inflammation, obesity, and thrombosis
    Fahumiya Samad
    Department of Cell Biology, Torrey Pines Institute for Molecular Studies, San Diego, CA and
    Blood 122:3415-22. 2013
    ..Furthermore, TF-induced signaling could provide new therapeutic targets for drug development at the intersection between obesity, inflammation, and thrombosis. ..
  38. ncbi request reprint Regulation of angiogenesis by tissue factor cytoplasmic domain signaling
    Mattias Belting
    Department of Immunology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Med 10:502-9. 2004
    ..Targeting the TF-VIIa signaling pathway may thus enhance the efficacy of angiostatic treatments for cancer and neovascular eye diseases...
  39. ncbi request reprint Regulation of tissue factor--mediated initiation of the coagulation cascade by cell surface grp78
    Gourab Bhattacharjee
    Department of Immunology, The Scripps Research Institute, SP258, La Jolla, CA 92037, USA
    Arterioscler Thromb Vasc Biol 25:1737-43. 2005
    ..To test the hypothesis that Grp78 negatively regulates cell surface tissue factor (TF) procoagulant activity and whether this is mediated by physical interaction...
  40. ncbi request reprint Protease-activated receptors (PAR1 and PAR2) contribute to tumor cell motility and metastasis
    Xiaoli Shi
    Cancer Biology Division, La Jolla Institute for Molecular Medicine, 4570 Executive Drive, San Diego, CA 92121, USA
    Mol Cancer Res 2:395-402. 2004
    ..Together, these data confirm a role for PAR1 in migration and metastasis and demonstrate an unexpected role for PAR2 in thrombin-dependent tumor cell migration and in metastasis...
  41. pmc Mechanistic studies of the immunochemical termination of self-tolerance with unnatural amino acids
    Jan Grünewald
    Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 106:4337-42. 2009
    ..These findings suggest that this may be a relatively general approach to generate effective immunotherapeutics against cancer-associated or other weakly immunogenic antigens...
  42. ncbi request reprint The tissue factor/factor VIIa/factor Xa complex: a model built by docking and site-directed mutagenesis
    Brian V Norledge
    The Scripps Research Institute, La Jolla, California 92037, USA
    Proteins 53:640-8. 2003
    ....
  43. pmc Tissue factor and PAR2 signaling in the tumor microenvironment
    Florence Schaffner
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA
    Arterioscler Thromb Vasc Biol 29:1999-2004. 2009
    ....
  44. pmc Disulfide isomerization switches tissue factor from coagulation to cell signaling
    Jasimuddin Ahamed
    Department of Immunology, The Scripps Research Institute, SP258, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 103:13932-7. 2006
    ..These experiments delineate an upstream regulatory mechanism that controls TF function, and they provide initial evidence that TF.VIIa signaling can be specifically inhibited with minimal effects on coagulation...
  45. pmc P2X7 receptor signaling contributes to tissue factor-dependent thrombosis in mice
    Christian Furlan-Freguia
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California 92037, USA
    J Clin Invest 121:2932-44. 2011
    ..These data suggest that PDI regulates a critical P2X7 receptor-dependent signaling pathway that generates prothrombotic TF, defining a link between inflammation and thrombosis with potential implications for antithrombotic therapy...
  46. pmc Cooperation of tissue factor cytoplasmic domain and PAR2 signaling in breast cancer development
    Florence Schaffner
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA
    Blood 116:6106-13. 2010
    ....
  47. pmc Cross-talk of integrin alpha3beta1 and tissue factor in cell migration
    Andrea Dorfleutner
    Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Biol Cell 15:4416-25. 2004
    ..Thus, TF influences integrin-mediated migration through cooperative intra- and extracellular interactions and phosphorylation regulates TF's function in cell motility...
  48. ncbi request reprint Signaling of the tissue factor coagulation pathway in angiogenesis and cancer
    Mattias Belting
    Department of Immunology, The Scripps Research Institute, 10550 N Torrey Pines Rd, La Jolla, CA 92037, USA
    Arterioscler Thromb Vasc Biol 25:1545-50. 2005
    ..Here we review the molecular mechanisms of tissue factor pathways in angiogenesis and tumorigenesis with emphasis on the intriguing role for tissue factor cytoplasmic domain signaling...
  49. ncbi request reprint Thrombin generation and the pathogenesis of cancer
    Wolfram Ruf
    Scripps Research Institute and the La Jolla Institute for Molecular Medicine, San Diego, California 92037, USA
    Semin Thromb Hemost 32:61-8. 2006
    ..Transmembrane serine proteases as well as matrix metalloproteinase are prime candidates for accessory pathways to regulate metastasis, tumor expansion, and angiogenesis dependent on specific features of the local tumor microenvironment...
  50. pmc Infrared fluorescence for vascular barrier breach in vivo--a novel method for quantitation of albumin efflux
    Annette Von Drygalski
    Department of Medicine, University of California San Diego, San Diego, California, USA
    Thromb Haemost 108:981-91. 2012
    ..In summary, EB-IRF is a novel, highly sensitive, rapid, and convenient method for the relative quantification of EB in intact organs of treatment versus control mice...
  51. ncbi request reprint Tissue factor proangiogenic signaling in cancer progression
    Florence Schaffner
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA
    Thromb Res 129:S127-31. 2012
    ....
  52. ncbi request reprint A hybrid fibronectin motif protein as an integrin targeting selective tumor vascular thrombogen
    Cheng Liu
    Department of Immunology, Scripps Research Institute, La Jolla, California 92037, USA
    Mol Cancer Ther 3:793-801. 2004
    ..This response can produce local tumor infarction followed by intratumoral ischemia-reperfusion injury, inflammation, and a local host tumor eradicative response...
  53. ncbi request reprint Regulation of tissue factor cytoplasmic domain phosphorylation by palmitoylation
    Andrea Dorfleutner
    Department of Immunology, C204, The Scripps Research Institute, 10550 North Torrey Pines Rd, La Jolla, CA 92037, USA
    Blood 102:3998-4005. 2003
    ..Thus, cell surface location-dependent phosphorylation of the TF cytoplasmic domain is regulated at multiple levels...
  54. pmc Cell surface proteomics identifies molecules functionally linked to tumor cell intravasation
    Erin M Conn
    Departments of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 283:26518-27. 2008
    ..Overall, our cell surface proteomic analysis provides a powerful tool for identification of specific cell membrane molecules that contribute functionally to intravasation and metastasis in vivo...
  55. ncbi request reprint Protease-activated receptor signaling in the regulation of inflammation
    Wolfram Ruf
    Department of Immunology, The Scripps Research Institute, La Jolla, CA, USA
    Crit Care Med 32:S287-92. 2004
    ..To discuss recent studies addressing the relationship between protease-activated receptor signaling, coagulation, and inflammation...
  56. ncbi request reprint Role of zymogenicity-determining residues of coagulation factor VII/VIIa in cofactor interaction and macromolecular substrate recognition
    Ramona J Petrovan
    Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
    Biochemistry 41:9302-9. 2002
    ....
  57. ncbi request reprint Gene induction by coagulation factor Xa is mediated by activation of protease-activated receptor 1
    M Riewald
    Department of Immunology and Vascular Biology, The Scripps Research Institute, La Jolla, CA, USA
    Blood 97:3109-16. 2001
    ....
  58. doi request reprint Protease-activated receptor 2 signaling in inflammation
    Andrea S Rothmeier
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA
    Semin Immunopathol 34:133-49. 2012
    ....
  59. ncbi request reprint Protease-activated receptor 2-dependent phosphorylation of the tissue factor cytoplasmic domain
    Jasimuddin Ahamed
    Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 279:23038-44. 2004
    ..Thus, PAR2 signaling specifically phosphorylates TF in a receptor cross-talk that distinguishes upstream from downstream coagulation protease signaling...
  60. pmc Mechanistic coupling of protease signaling and initiation of coagulation by tissue factor
    M Riewald
    Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 98:7742-7. 2001
    ....
  61. ncbi request reprint The structural basis of function of the TF. VIIa complex in the cellular initiation of coagulation
    T S Edgington
    Department of Immunology, Scripps Research Institute, La Jolla, CA 92037, USA
    Thromb Haemost 78:401-5. 1997
    ..Docking of the VIIa Gla-domain to the latter region of TF appears to contribute to substrate assembly. The current data suggest an extended embrace between TF and VIIa to form the bimolecular enzyme TF.VIIa...
  62. pmc Requirement for binding of catalytically active factor VIIa in tissue factor-dependent experimental metastasis
    B M Mueller
    Department of Immunology, Scripps Research Institute, La Jolla, California 92037, USA
    J Clin Invest 101:1372-8. 1998
    ..Thus, extracellular functions of the catalytically active TF-VIIa complex cooperate with specific functions of the TF cytoplasmic domain to support the complex process of hematogenous tumor cell dissemination...
  63. pmc Immunochemical termination of self-tolerance
    Jan Grünewald
    Department of Chemistry, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 105:11276-80. 2008
    ..This approach may provide a general method for inducing an antibody response to specific epitopes of self- and foreign antigens that lead to a neutralizing immune response...
  64. ncbi request reprint Tissue factor-dependent coagulation protease signaling in acute lung injury
    Wolfram Ruf
    Department of Immunology, C204, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Crit Care Med 31:S231-7. 2003
    ..To interpret preclinical and clinical data on therapeutic intervention of the coagulation cascade, focusing on the principles of proteolytic cell signaling of the coagulant and anticoagulant pathways...
  65. ncbi request reprint Residue Met(156) contributes to the labile enzyme conformation of coagulation factor VIIa
    R J Petrovan
    Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 276:6616-20. 2001
    ..The mutation thus selectively influenced the catalytic activity of free VIIa, identifying the Met(156) residue position as a determinant for the zymogen-like properties of free VIIa...
  66. pmc A role for tissue factor in cell adhesion and migration mediated by interaction with actin-binding protein 280
    I Ott
    Departments of Immunology and Vascular Biology, IMM 17, The Scripps Research Institute, La Jolla, California 92037, USA
    J Cell Biol 140:1241-53. 1998
    ..The specific interaction of the TF cytoplasmic domain with ABP-280 provides a molecular pathway by which TF supports tumor cell metastasis and vascular remodeling...
  67. ncbi request reprint Plasmin-induced migration of endothelial cells. A potential target for the anti-angiogenic action of angiostatin
    Takehiko Tarui
    Department of Cell Biology, VB 6, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    J Biol Chem 277:33564-70. 2002
    ..These results suggest that plasmin-induced migration of endothelial cells requires alphavbeta(3) and the catalytic activity of plasmin and that this process is a potential target for the inhibitory activity of angiostatin...
  68. ncbi request reprint The mechanism of an inhibitory antibody on TF-initiated blood coagulation revealed by the crystal structures of human tissue factor, Fab 5G9 and TF.G9 complex
    M Huang
    Department of Molecular Biology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92027, USA
    J Mol Biol 275:873-94. 1998
    ..The TF. 5G9 structure provides insights into the mechanism by which the antibody 5G9 inhibits formation of the TF.VIIa.X ternary complex...
  69. pmc HIF2α-Sp1 interaction mediates a deacetylation-dependent FVII-gene activation under hypoxic conditions in ovarian cancer cells
    Shiro Koizume
    Molecular Pathology and Genetics Division, Kanagawa Cancer Center Research Institute, 1 1 2 Nakao, Asahi ku, Yokohama 241 0815, Japan
    Nucleic Acids Res 40:5389-401. 2012
    ..These results suggest the presence of a stress-responsive transcription mediated by the HIF2α/Sp1/HDAC4 network and explain how CCC shed their procoagulant activity under hypoxia...
  70. ncbi request reprint Tissue factor pathway inhibitor-2 (TFPI-2) recognizes the complement and kininogen binding protein gC1qR/p33 (gC1qR): implications for vascular inflammation
    Ellinor I B Peerschke
    New York Presbyterian Hospital, Weill Cornell Center, 525 East 68th Street, Room F715, New York 10021, USA
    Thromb Haemost 92:811-9. 2004
    ..Taken together, these data suggest that gC1qR may participate in tissue remodeling and inflammation by localizing TFPI-2 to the pericellular environment to modulate local protease activity and regulate HK activation...
  71. ncbi request reprint Plasmin-induced migration requires signaling through protease-activated receptor 1 and integrin alpha(9)beta(1)
    Mousumi Majumdar
    Department of Dermatology, University of California Davis Medical Center, Sacramento, California 95817, USA
    J Biol Chem 279:37528-34. 2004
    ..These results suggest that plasmin induces migration by kringle-mediated binding to alpha(9)beta(1) and simultaneous proteolytic activation of PAR-1...
  72. ncbi request reprint PAR1 signaling: more good than harm?
    Wolfram Ruf
    Nat Med 9:258-60. 2003
  73. ncbi request reprint Flow perturbation is linked to endothelial par signaling
    Wolfram Ruf
    Arterioscler Thromb Vasc Biol 26:962-4. 2006
  74. ncbi request reprint Evidence for activation of tissue factor by an allosteric disulfide bond
    Vivien M Chen
    Centre for Vascular Research, University of New South Wales, Sydney, NSW 2052, Australia
    Biochemistry 45:12020-8. 2006
    ..It is likely that formation of this disulfide bond changes the conformation of the domain that facilitates productive binding of factors IX and X...
  75. ncbi request reprint Activation of cancer cell migration and invasion by ectopic synthesis of coagulation factor VII
    Shiro Koizume
    Molecular Pathology and Genetics Division, Kanagawa Cancer Center Research Institute, Asahi ku, Yokohama 241 0815, Japan
    Cancer Res 66:9453-60. 2006
    ..This study shows that ectopic synthesis of fVII by cancer cells is sufficient to support proinvasive factor Xa-mediated protease-activated receptor-1 signaling and that this pathway is inducible under hypoxia...
  76. pmc Regulation of macrophage procoagulant responses by the tissue factor cytoplasmic domain in endotoxemia
    Jasimuddin Ahamed
    The Rockefeller University, New York, NY, USA
    Blood 109:5251-9. 2007
    ..The present study demonstrates a new role of the TF cytoplasmic domain in an autoregulatory pathway that controls LPS-induced TF expression in macrophages and procoagulant responses in endotoxemia...
  77. ncbi request reprint Role of PAR2 in murine pulmonary pseudomonal infection
    Theo J Moraes
    Academic Affairs, National Jewish Medical and Research Center, 1400 Jackson Street, Denver, CO 80206, USA
    Am J Physiol Lung Cell Mol Physiol 294:L368-77. 2008
    ..aeruginosa. Our data suggest that a deficiency in IFN-gamma production and impaired bacterial phagocytosis are two potential mechanisms responsible for this defect...
  78. pmc Neutrophil activation by the tissue factor/Factor VIIa/PAR2 axis mediates fetal death in a mouse model of antiphospholipid syndrome
    Patricia Redecha
    Hospital for Special Surgery, Weill Medical College of Cornell University, New York, New York, USA
    J Clin Invest 118:3453-61. 2008
    ..Our results suggest that TF/FVIIa/PAR2 signaling mediates neutrophil activation and fetal death in APS and that statins may be a good treatment for women with aPL-induced pregnancy complications...
  79. pmc Activated protein C in sepsis: the promise of nonanticoagulant activated protein C
    Hartmut Weiler
    aBlood Research Institute, BloodCenter of Wisconsin, Milwaukee, Wisconsin, USA
    Curr Opin Hematol 15:487-93. 2008
    ..To discuss the potential use of recombinant activated protein C (aPC) variants with altered bioactivity in sepsis therapy...

Research Grants34

  1. Functional Cooperation of Tissue Factor and Integrins
    Wolfram Ruf; Fiscal Year: 2005
    ..Together, these experiments will characterize a novel pathway of integrin cooperation with TF of relevance for endothelial cell migration. ..
  2. NEW GENETIC MODELS FOR TISSUE FACTOR SIGNALING
    Wolfram Ruf; Fiscal Year: 2005
    ..Identification of such markers will facilitate the quantitation of coagulation signaling in vivo and thus advance clinical efforts to design therapeutic intervention in coagulant cell signaling pathways . ..
  3. TARGETING SIGNALING OF THE TISSUE FACTOR PATHWAY
    Wolfram Ruf; Fiscal Year: 2005
    ..abstract_text> ..
  4. Functional Cooperation of Tissue Factor and Integrins
    Wolfram Ruf; Fiscal Year: 2006
    ..Together, these experiments will characterize a novel pathway of integrin cooperation with TF of relevance for endothelial cell migration. ..
  5. NEW GENETIC MODELS FOR TISSUE FACTOR SIGNALING
    Wolfram Ruf; Fiscal Year: 2006
    ..Identification of such markers will facilitate the quantitation of coagulation signaling in vivo and thus advance clinical efforts to design therapeutic intervention in coagulant cell signaling pathways . ..
  6. TARGETING SIGNALING OF THE TISSUE FACTOR PATHWAY
    Wolfram Ruf; Fiscal Year: 2006
    ..abstract_text> ..
  7. Functional Cooperation of Tissue Factor and Integrins
    Wolfram Ruf; Fiscal Year: 2007
    ..Together, these experiments will characterize a novel pathway of integrin cooperation with TF of relevance for endothelial cell migration. ..
  8. NEW GENETIC MODELS FOR TISSUE FACTOR SIGNALING
    Wolfram Ruf; Fiscal Year: 2007
    ..Identification of such markers will facilitate the quantitation of coagulation signaling in vivo and thus advance clinical efforts to design therapeutic intervention in coagulant cell signaling pathways . ..
  9. TARGETING SIGNALING OF THE TISSUE FACTOR PATHWAY
    Wolfram Ruf; Fiscal Year: 2007
    ..abstract_text> ..
  10. Functional Cooperation of Tissue Factor and Integrins
    Wolfram Ruf; Fiscal Year: 2009
    ..Because a prototypic inhibitor of this pathway is effective to attenuate tumor growth in preclinical models, the proposed basic mechanistic studies facilitate a rational translation of this novel concept into the clinic. ..
  11. TF Signaling in Systemic Inflammation
    Wolfram Ruf; Fiscal Year: 2009
    ....
  12. PAR SIGNALING AND PROTECTIVE PATHWAYS IN INFLAMMATION AND SEPSIS
    Wolfram Ruf; Fiscal Year: 2009
    ..3. ..
  13. Functional Cooperation of Tissue Factor and Integrins
    Wolfram Ruf; Fiscal Year: 2010
    ..Because a prototypic inhibitor of this pathway is effective to attenuate tumor growth in preclinical models, the proposed basic mechanistic studies facilitate a rational translation of this novel concept into the clinic. ..
  14. TF Signaling in Systemic Inflammation
    Wolfram Ruf; Fiscal Year: 2010
    ....
  15. NEW GENETIC MODELS FOR TISSUE FACTOR SIGNALING
    Wolfram Ruf; Fiscal Year: 2004
    ..Identification of such markers will facilitate the quantitation of coagulation signaling in vivo and thus advance clinical efforts to design therapeutic intervention in coagulant cell signaling pathways . ..
  16. ALLOSTERIC REGULATION OF FACTOR VII FUNCTION
    Wolfram Ruf; Fiscal Year: 1999
    ..The detailed insight into allosteric regulation and determinants of substrate specificity in VIIa may provide novel strategies for therapeutic intervention in inflammatory and thrombotic diseases. ..
  17. SIGNALING OF TISSUE FACTOR IN CELL ADHESION
    Wolfram Ruf; Fiscal Year: 1999
    ..In the long term, elucidation of the molecular details of TF signaling will guide novel therapeutic approaches that intervene with functions of TF in metastasis and angiogenesis. ..
  18. ALLOSTERIC REGULATION OF FACTOR VII FUNCTION
    Wolfram Ruf; Fiscal Year: 2000
    ..The detailed insight into allosteric regulation and determinants of substrate specificity in VIIa may provide novel strategies for therapeutic intervention in inflammatory and thrombotic diseases. ..
  19. SIGNALING OF TISSUE FACTOR IN CELL ADHESION
    Wolfram Ruf; Fiscal Year: 2000
    ..In the long term, elucidation of the molecular details of TF signaling will guide novel therapeutic approaches that intervene with functions of TF in metastasis and angiogenesis. ..
  20. ALLOSTERIC REGULATION OF FACTOR VII FUNCTION
    Wolfram Ruf; Fiscal Year: 2001
    ..The detailed insight into allosteric regulation and determinants of substrate specificity in VIIa may provide novel strategies for therapeutic intervention in inflammatory and thrombotic diseases. ..
  21. SIGNALING OF TISSUE FACTOR IN CELL ADHESION
    Wolfram Ruf; Fiscal Year: 2001
    ..In the long term, elucidation of the molecular details of TF signaling will guide novel therapeutic approaches that intervene with functions of TF in metastasis and angiogenesis. ..
  22. ALLOSTERIC REGULATION OF FACTOR VII FUNCTION
    Wolfram Ruf; Fiscal Year: 2002
    ..The detailed insight into allosteric regulation and determinants of substrate specificity in VIIa may provide novel strategies for therapeutic intervention in inflammatory and thrombotic diseases. ..
  23. Structural Basis of Protease Signaling by Vlla
    Wolfram Ruf; Fiscal Year: 2003
    ..abstract_text> ..
  24. Functional Cooperation of Tissue Factor and Integrins
    Wolfram Ruf; Fiscal Year: 2004
    ..Together, these experiments will characterize a novel pathway of integrin cooperation with TF of relevance for endothelial cell migration. ..
  25. PAR SIGNALING AND PROTECTIVE PATHWAYS IN INFLAMMATION AND SEPSIS
    Wolfram Ruf; Fiscal Year: 2010
    ..3. ..