Research Topics
Genomes and Genes
| Wolfram RufSummaryAffiliation: The Scripps Research Institute Country: USA Publications
Research Grants
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Detail Information
Publications
Tissue factor and cancerWolfram Ruf
Department of Immunology and Microbial Science, SP258, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, United States
Thromb Res 130:S84-7. 2012..Basic research continues to identify pivotal molecular interactions in these processes and provides potential leads for targeting specific tumor promoting pathways associated with hemostasis and thrombosis...
Role of thiol pathways in TF procoagulant regulationWolfram Ruf
Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Thromb Res 129:S11-2. 2012..Genetic deletion of P2X7 signaling attenuates FeCl(3)-induced carotid artery thrombosis in mice, indicating that TF prothrombotic activity is regulated by specific cell signaling pathways in vivo...- Science review: role of coagulation protease cascades in sepsisMatthias Riewald
Department of Immunology C204, The Scripps Research Institute, La Jolla, California, USA
Crit Care 7:123-9. 2003..This dichotomy is of relevance for the interpretation of preclinical and clinical data that document nonuniform responses to anticoagulant strategies in sepsis therapy... 
Tissue factor and PAR signaling in tumor progressionWolfram Ruf
Department of Immunology, Scripps Research Institute, La Jolla, CA, USA
Thromb Res 120:S7-12. 2007..A combination of experimental models of spontaneous and transplanted tumor development and metastasis start to delineate the role of TF in tumor progression and identified potential therapeutic approaches to target the TF pathway...- Tissue factor in cancer progression and angiogenesisWolfram Ruf
Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA
Thromb Res 125:S36-8. 2010..Thus, interruption of TF signaling represents a potential anti-angiogenic strategy that does not carry an increased risk of bleeding associated with prolonged inhibition of the TF coagulation pathway... - Specificity of coagulation factor signalingW Ruf
Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
J Thromb Haemost 1:1495-503. 2003..Protease receptors are thus major determinants of the biological outcome of coagulation factor signaling on vascular cells... - Vascular and dendritic cell coagulation signaling in sepsis progressionW Ruf
Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA
J Thromb Haemost 7:118-21. 2009.... - Tissue factor-dependent signaling in tumor biologyWolfram Ruf
Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
Pathophysiol Haemost Thromb 33:28-30. 2003 - Differential role of tissue factor pathway inhibitors 1 and 2 in melanoma vasculogenic mimicryWolfram Ruf
Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
Cancer Res 63:5381-9. 2003..Thus, TFPI-2 contributes to VM plasticity, whereas TFPI-1 has anticoagulant functions of relevance for perfusion of VM channels formed by TF-expressing melanoma cells... - Redundant signaling of tissue factor and thrombin in cancer progression?W Ruf
Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
J Thromb Haemost 5:1584-7. 2007 - Emerging roles of tissue factor in viral hemorrhagic feverWolfram Ruf
Department of Immunology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Trends Immunol 25:461-4. 2004..Thus, targeting TF appears to aid the antiviral immune response in hemorrhagic fevers, and further studies are encouraged to define how TF-dependent signaling regulates immunity... - Is APC activation of endothelial cell PAR1 important in severe sepsis?: YesW Ruf
Department of Immunology, SP258, The Scripps Research Institute, La Jolla, CA 92037, USA
J Thromb Haemost 3:1912-4. 2005 - New players in the sepsis-protective activated protein C pathwayWolfram Ruf
Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California 92037, USA
J Clin Invest 120:3084-7. 2010.... - The interaction of activated factor VII with tissue factor: insight into the mechanism of cofactor-mediated activation of activated factor VIIW Ruf
Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
Blood Coagul Fibrinolysis 9:S73-8. 1998..Combining these findings with the results of mutational analysis of TF, a picture emerges whereby multiple domains of both cofactor and enzyme provide contacts for the assembly of macromolecular substrate... - Molecular regulation of blood clotting in tumor biologyW Ruf
Department of Immunology and Vascular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Haemostasis 31:5-7. 2001..e. TF-VIIa-Xa, generates autocrine cell signaling though protease activated receptors. A cooperation of the TF cytoplasmic domain with protease signaling may explain the diverse contributions of TF to metastasis and angiogenesis... - Diverse functions of protease receptor tissue factor in inflammation and metastasisW Ruf
Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
Immunol Res 21:289-92. 2000.... - Endogenous EPCR/aPC-PAR1 signaling prevents inflammation-induced vascular leakage and lethalityFrank Niessen
Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA
Blood 113:2859-66. 2009..Thus, the activating proteases and selectivity in coupling to S1P receptor subtypes determine vascular PAR1 signaling specificity in systemic inflammatory response syndromes in vivo... - The endothelial protein C receptor supports tissue factor ternary coagulation initiation complex signaling through protease-activated receptorsJennifer Disse
Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California 92037, USA
J Biol Chem 286:5756-67. 2011.... - Protease-activated receptor-1 signaling by activated protein C in cytokine-perturbed endothelial cells is distinct from thrombin signalingMatthias Riewald
Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
J Biol Chem 280:19808-14. 2005..These data may explain how APC can be therapeutically protective through the EPCR-PAR1 signaling despite ongoing thrombin generation due to disseminated intravascular coagulopathy... - Inhibition of tissue factor signaling suppresses tumor growthHenri H Versteeg
Department of Immunology, SP258, Scripps Research Institute, La Jolla, CA 92037, USA
Blood 111:190-9. 2008..These results show that tumor cell TF-PAR2 signaling is crucial for tumor growth and suggest that anti-TF strategies can be applied in cancer therapy with minor impairment of TF-dependent hemostatic pathways... - Factor Xa binding to annexin 2 mediates signal transduction via protease-activated receptor 1Gourab Bhattacharjee
Scripps Research Institute, SP 258, 10550 N Torrey Pines Rd, La Jolla, CA 92037, USA
Circ Res 102:457-64. 2008..We propose that annexin 2 serves to regulate factor Xa signaling specifically in the absence of cell surface TF and may thus play physiological or pathological roles when factor Xa is generated in a TF-depleted environment... - Regulation of tissue factor-induced signaling by endogenous and recombinant tissue factor pathway inhibitor 1Jasimuddin Ahamed
Department of Immunology, SP258, The Scripps Research Institute, 10550 North Torrey Pines Rd, La Jolla, CA 92037, USA
Blood 105:2384-91. 2005.... - Organ-specific protection against lipopolysaccharide-induced vascular leak is dependent on the endothelial protein C receptorAnnette Von Drygalski
Department of Molecular and Experimental Medicine, The Scripps Research Institute, MEM 180, 10550 North Torrey Pines Rd, La Jolla, CA 92037, USA
Arterioscler Thromb Vasc Biol 33:769-76. 2013..CONCLUSIONS: This study demonstrates an important protective role for EPCR in vivo against vascular leakage during inflammation and suggests that EPCR-dependent vascular protection is organ-specific... - Protease-activated receptor (PAR) 2, but not PAR1, signaling promotes the development of mammary adenocarcinoma in polyoma middle T miceHenri H Versteeg
Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
Cancer Res 68:7219-27. 2008..This study establishes that protease signaling contributes to mammary tumor development and that PAR2, rather than the thrombin receptor PAR1, plays a crucial role in the angiogenic switch... - Role of protease activated receptor 1 and 2 signaling in hypoxia-induced angiogenesisHannele Uusitalo-Jarvinen
Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Arterioscler Thromb Vasc Biol 27:1456-62. 2007..The TF-VIIa complex also directly cleaves PAR2 and promotes angiogenesis in vitro in TF cytoplasmic domain-deleted (TF(deltaCT)) mice. Here we address the effect of PAR1 and PAR2 deficiency on angiogenesis in vivo... - Dendritic cell PAR1-S1P3 signalling couples coagulation and inflammationFrank Niessen
Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
Nature 452:654-8. 2008..Thus, activation of dendritic cells by coagulation in the lymphatics emerges as a previously unknown mechanism that promotes systemic inflammation and lethality in decompensated innate immune responses... - Orchestration of coagulation protease signaling by tissue factorMatthias Riewald
Department of Immunology C204, The Scripps Research Institute, La Jolla, CA 92037, USA
Trends Cardiovasc Med 12:149-54. 2002..Targeting inflammatory cell signaling events of the coagulation system may become an important aspect of efforts to improve antithrombotic therapy... - Molecular dynamics simulations and functional characterization of the interactions of the PAR2 ectodomain with factor VIIaQing Zhang
Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
Proteins 77:559-69. 2009.... - Tissue factor coagulant function is enhanced by protein-disulfide isomerase independent of oxidoreductase activityHenri H Versteeg
Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
J Biol Chem 282:25416-24. 2007..PDI enhanced TF coagulant activity on microvesicles shed from cells, suggesting that PDI plays a role as an activating chaperone for circulating cryptic TF... - Activation of endothelial cell protease activated receptor 1 by the protein C pathwayMatthias Riewald
Department of Immunology, C204, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Science 296:1880-2. 2002..Thus, the prototypical thrombin receptor is the target for EPCR-dependent APC signaling, suggesting a role for this receptor cascade in protection from sepsis... - Emerging insights in tissue factor-dependent signaling eventsHenri H Versteeg
Department of Immunology, The Scripps Institute, La Jolla, CA 92037, USA
Semin Thromb Hemost 32:24-32. 2006..In this review, we relate our current understanding of direct TF signaling pathways to the emerging roles of TF in (patho)physiology... - Tissue factor and protease-activated receptor signaling in cancerFlorence Schaffner
Research Associate, Department of Immunology, The Scripps Research Institute, La Jolla, California, USA
Semin Thromb Hemost 34:147-53. 2008.... - Activated protein C signals through the thrombin receptor PAR1 in endothelial cellsMatthias Riewald
Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
J Endotoxin Res 9:317-21. 2003..These data demonstrate unexpected protective functions of the major thrombin receptor PAR1 in endothelial cells... - Tissue factor-protease-activated receptor 2 signaling promotes diet-induced obesity and adipose inflammationLeylla Badeanlou
Department of Cell Biology, Torrey Pines Institute for Molecular Studies, San Diego, California, USA
Nat Med 17:1490-7. 2011..Thus, inhibition of tissue factor signaling is a potential therapeutic avenue for improving impaired metabolism and insulin resistance in obesity... - Tissue factor and PAR2 signaling in the tumor microenvironmentFlorence Schaffner
Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA
Arterioscler Thromb Vasc Biol 29:1999-2004. 2009.... - Disulfide isomerization switches tissue factor from coagulation to cell signalingJasimuddin Ahamed
Department of Immunology, The Scripps Research Institute, SP258, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 103:13932-7. 2006..These experiments delineate an upstream regulatory mechanism that controls TF function, and they provide initial evidence that TF.VIIa signaling can be specifically inhibited with minimal effects on coagulation... - The tissue factor/factor VIIa/factor Xa complex: a model built by docking and site-directed mutagenesisBrian V Norledge
The Scripps Research Institute, La Jolla, California 92037, USA
Proteins 53:640-8. 2003.... - Regulation of angiogenesis by tissue factor cytoplasmic domain signalingMattias Belting
Department of Immunology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
Nat Med 10:502-9. 2004..Targeting the TF-VIIa signaling pathway may thus enhance the efficacy of angiostatic treatments for cancer and neovascular eye diseases... - Mechanistic studies of the immunochemical termination of self-tolerance with unnatural amino acidsJan Grünewald
Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 106:4337-42. 2009..These findings suggest that this may be a relatively general approach to generate effective immunotherapeutics against cancer-associated or other weakly immunogenic antigens... - Thrombin generation and the pathogenesis of cancerWolfram Ruf
Scripps Research Institute and the La Jolla Institute for Molecular Medicine, San Diego, California 92037, USA
Semin Thromb Hemost 32:61-8. 2006..Transmembrane serine proteases as well as matrix metalloproteinase are prime candidates for accessory pathways to regulate metastasis, tumor expansion, and angiogenesis dependent on specific features of the local tumor microenvironment... - Regulation of tissue factor--mediated initiation of the coagulation cascade by cell surface grp78Gourab Bhattacharjee
Department of Immunology, The Scripps Research Institute, SP258, La Jolla, CA 92037, USA
Arterioscler Thromb Vasc Biol 25:1737-43. 2005..To test the hypothesis that Grp78 negatively regulates cell surface tissue factor (TF) procoagulant activity and whether this is mediated by physical interaction... - Signaling of the tissue factor coagulation pathway in angiogenesis and cancerMattias Belting
Department of Immunology, The Scripps Research Institute, 10550 N Torrey Pines Rd, La Jolla, CA 92037, USA
Arterioscler Thromb Vasc Biol 25:1545-50. 2005..Here we review the molecular mechanisms of tissue factor pathways in angiogenesis and tumorigenesis with emphasis on the intriguing role for tissue factor cytoplasmic domain signaling... - Cooperation of tissue factor cytoplasmic domain and PAR2 signaling in breast cancer developmentFlorence Schaffner
Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA
Blood 116:6106-13. 2010.... - Cross-talk of integrin alpha3beta1 and tissue factor in cell migrationAndrea Dorfleutner
Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
Mol Biol Cell 15:4416-25. 2004..Thus, TF influences integrin-mediated migration through cooperative intra- and extracellular interactions and phosphorylation regulates TF's function in cell motility... - Infrared fluorescence for vascular barrier breach in vivo--a novel method for quantitation of albumin effluxAnnette Von Drygalski
Department of Medicine, University of California San Diego, San Diego, California, USA
Thromb Haemost 108:981-91. 2012..In summary, EB-IRF is a novel, highly sensitive, rapid, and convenient method for the relative quantification of EB in intact organs of treatment versus control mice... - Tissue factor proangiogenic signaling in cancer progressionFlorence Schaffner
Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA
Thromb Res 129:S127-31. 2012.... - A hybrid fibronectin motif protein as an integrin targeting selective tumor vascular thrombogenCheng Liu
Department of Immunology, Scripps Research Institute, La Jolla, California 92037, USA
Mol Cancer Ther 3:793-801. 2004..This response can produce local tumor infarction followed by intratumoral ischemia-reperfusion injury, inflammation, and a local host tumor eradicative response... - Regulation of tissue factor cytoplasmic domain phosphorylation by palmitoylationAndrea Dorfleutner
Department of Immunology, C204, The Scripps Research Institute, 10550 North Torrey Pines Rd, La Jolla, CA 92037, USA
Blood 102:3998-4005. 2003..Thus, cell surface location-dependent phosphorylation of the TF cytoplasmic domain is regulated at multiple levels... - P2X7 receptor signaling contributes to tissue factor-dependent thrombosis in miceChristian Furlan-Freguia
Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California 92037, USA
J Clin Invest 121:2932-44. 2011..These data suggest that PDI regulates a critical P2X7 receptor-dependent signaling pathway that generates prothrombotic TF, defining a link between inflammation and thrombosis with potential implications for antithrombotic therapy... - Protease-activated receptor signaling in the regulation of inflammationWolfram Ruf
Department of Immunology, The Scripps Research Institute, La Jolla, CA, USA
Crit Care Med 32:S287-92. 2004..Further insight into this question will require the development or use of additional biomarkers for assessing pharmacologic interference with coagulation-related cell-signaling pathways... - Role of zymogenicity-determining residues of coagulation factor VII/VIIa in cofactor interaction and macromolecular substrate recognitionRamona J Petrovan
Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
Biochemistry 41:9302-9. 2002.... - Gene induction by coagulation factor Xa is mediated by activation of protease-activated receptor 1M Riewald
Department of Immunology and Vascular Biology, The Scripps Research Institute, La Jolla, CA, USA
Blood 97:3109-16. 2001.... - Endothelial protein C receptor function in murine and human breast cancer developmentFlorence Schaffner
Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California, United States of America
PLoS ONE 8:e61071. 2013.... - Protease-activated receptor 2 signaling in inflammationAndrea S Rothmeier
Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA
Semin Immunopathol 34:133-49. 2012.... - Protease-activated receptor 2-dependent phosphorylation of the tissue factor cytoplasmic domainJasimuddin Ahamed
Scripps Research Institute, La Jolla, California 92037, USA
J Biol Chem 279:23038-44. 2004..Thus, PAR2 signaling specifically phosphorylates TF in a receptor cross-talk that distinguishes upstream from downstream coagulation protease signaling... - Cell surface proteomics identifies molecules functionally linked to tumor cell intravasationErin M Conn
Departments of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
J Biol Chem 283:26518-27. 2008..Overall, our cell surface proteomic analysis provides a powerful tool for identification of specific cell membrane molecules that contribute functionally to intravasation and metastasis in vivo... - Mechanistic coupling of protease signaling and initiation of coagulation by tissue factorM Riewald
Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 98:7742-7. 2001.... - Requirement for binding of catalytically active factor VIIa in tissue factor-dependent experimental metastasisB M Mueller
Department of Immunology, Scripps Research Institute, La Jolla, California 92037, USA
J Clin Invest 101:1372-8. 1998..Thus, extracellular functions of the catalytically active TF-VIIa complex cooperate with specific functions of the TF cytoplasmic domain to support the complex process of hematogenous tumor cell dissemination... - The structural basis of function of the TF. VIIa complex in the cellular initiation of coagulationT S Edgington
Department of Immunology, Scripps Research Institute, La Jolla, CA 92037, USA
Thromb Haemost 78:401-5. 1997..Docking of the VIIa Gla-domain to the latter region of TF appears to contribute to substrate assembly. The current data suggest an extended embrace between TF and VIIa to form the bimolecular enzyme TF.VIIa... - Tissue factor-dependent coagulation protease signaling in acute lung injuryWolfram Ruf
Department of Immunology, C204, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA
Crit Care Med 31:S231-7. 2003..Therefore, in the vasculature, inflammatory signaling by the tissue factor initiation complex is favored over thrombin signaling... - Immunochemical termination of self-toleranceJan Grünewald
Department of Chemistry, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 105:11276-80. 2008..This approach may provide a general method for inducing an antibody response to specific epitopes of self- and foreign antigens that lead to a neutralizing immune response... - A role for tissue factor in cell adhesion and migration mediated by interaction with actin-binding protein 280I Ott
Departments of Immunology and Vascular Biology, IMM 17, The Scripps Research Institute, La Jolla, California 92037, USA
J Cell Biol 140:1241-53. 1998..The specific interaction of the TF cytoplasmic domain with ABP-280 provides a molecular pathway by which TF supports tumor cell metastasis and vascular remodeling... - Residue Met(156) contributes to the labile enzyme conformation of coagulation factor VIIaR J Petrovan
Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
J Biol Chem 276:6616-20. 2001..The mutation thus selectively influenced the catalytic activity of free VIIa, identifying the Met(156) residue position as a determinant for the zymogen-like properties of free VIIa... - Plasmin-induced migration of endothelial cells. A potential target for the anti-angiogenic action of angiostatinTakehiko Tarui
Department of Cell Biology, VB-6, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA
J Biol Chem 277:33564-70. 2002..These results suggest that plasmin-induced migration of endothelial cells requires alphavbeta(3) and the catalytic activity of plasmin and that this process is a potential target for the inhibitory activity of angiostatin... - The mechanism of an inhibitory antibody on TF-initiated blood coagulation revealed by the crystal structures of human tissue factor, Fab 5G9 and TF.G9 complexM Huang
Department of Molecular Biology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92027, USA
J Mol Biol 275:873-94. 1998..The TF. 5G9 structure provides insights into the mechanism by which the antibody 5G9 inhibits formation of the TF.VIIa.X ternary complex... - HIF2α-Sp1 interaction mediates a deacetylation-dependent FVII-gene activation under hypoxic conditions in ovarian cancer cellsShiro Koizume
Molecular Pathology and Genetics Division, Kanagawa Cancer Center Research Institute, 1 1 2 Nakao, Asahi ku, Yokohama 241 0815, Japan
Nucleic Acids Res 40:5389-401. 2012..These results suggest the presence of a stress-responsive transcription mediated by the HIF2α/Sp1/HDAC4 network and explain how CCC shed their procoagulant activity under hypoxia... - Plasmin-induced migration requires signaling through protease-activated receptor 1 and integrin alpha(9)beta(1)Mousumi Majumdar
Department of Dermatology, University of California Davis Medical Center, Sacramento, California 95817, USA
J Biol Chem 279:37528-34. 2004..These results suggest that plasmin induces migration by kringle-mediated binding to alpha(9)beta(1) and simultaneous proteolytic activation of PAR-1... - Regulation of macrophage procoagulant responses by the tissue factor cytoplasmic domain in endotoxemiaJasimuddin Ahamed
The Rockefeller University, New York, NY, USA
Blood 109:5251-9. 2007..The present study demonstrates a new role of the TF cytoplasmic domain in an autoregulatory pathway that controls LPS-induced TF expression in macrophages and procoagulant responses in endotoxemia... - Role of PAR2 in murine pulmonary pseudomonal infectionTheo J Moraes
Academic Affairs, National Jewish Medical and Research Center, 1400 Jackson Street, Denver, CO 80206, USA
Am J Physiol Lung Cell Mol Physiol 294:L368-77. 2008..aeruginosa. Our data suggest that a deficiency in IFN-gamma production and impaired bacterial phagocytosis are two potential mechanisms responsible for this defect... - Activation of cancer cell migration and invasion by ectopic synthesis of coagulation factor VIIShiro Koizume
Molecular Pathology and Genetics Division, Kanagawa Cancer Center Research Institute, Asahi ku, Yokohama 241 0815, Japan
Cancer Res 66:9453-60. 2006..This study shows that ectopic synthesis of fVII by cancer cells is sufficient to support proinvasive factor Xa-mediated protease-activated receptor-1 signaling and that this pathway is inducible under hypoxia... - Evidence for activation of tissue factor by an allosteric disulfide bondVivien M Chen
Centre for Vascular Research, University of New South Wales, Sydney, NSW 2052, Australia
Biochemistry 45:12020-8. 2006..It is likely that formation of this disulfide bond changes the conformation of the domain that facilitates productive binding of factors IX and X... - Flow perturbation is linked to endothelial par signalingWolfram Ruf
Arterioscler Thromb Vasc Biol 26:962-4. 2006 - PAR1 signaling: more good than harm?Wolfram Ruf
Nat Med 9:258-60. 2003 - Tissue factor pathway inhibitor-2 (TFPI-2) recognizes the complement and kininogen binding protein gC1qR/p33 (gC1qR): implications for vascular inflammationEllinor I B Peerschke
New York Presbyterian Hospital, Weill Cornell Center, 525 East 68th Street, Room F715, New York 10021, USA
Thromb Haemost 92:811-9. 2004..Taken together, these data suggest that gC1qR may participate in tissue remodeling and inflammation by localizing TFPI-2 to the pericellular environment to modulate local protease activity and regulate HK activation... - Neutrophil activation by the tissue factor/Factor VIIa/PAR2 axis mediates fetal death in a mouse model of antiphospholipid syndromePatricia Redecha
Hospital for Special Surgery, Weill Medical College of Cornell University, New York, New York, USA
J Clin Invest 118:3453-61. 2008..Our results suggest that TF/FVIIa/PAR2 signaling mediates neutrophil activation and fetal death in APS and that statins may be a good treatment for women with aPL-induced pregnancy complications... - Protease-activated receptors (PAR1 and PAR2) contribute to tumor cell motility and metastasisXiaoli Shi
Cancer Biology Division, La Jolla Institute for Molecular Medicine, 4570 Executive Drive, San Diego, CA 92121, USA
Mol Cancer Res 2:395-402. 2004..Together, these data confirm a role for PAR1 in migration and metastasis and demonstrate an unexpected role for PAR2 in thrombin-dependent tumor cell migration and in metastasis... - Activated protein C in sepsis: the promise of nonanticoagulant activated protein CHartmut Weiler
aBlood Research Institute, BloodCenter of Wisconsin, Milwaukee, Wisconsin, USA
Curr Opin Hematol 15:487-93. 2008..To discuss the potential use of recombinant activated protein C (aPC) variants with altered bioactivity in sepsis therapy...
Research Grants
- Functional Cooperation of Tissue Factor and IntegrinsWolfram Ruf; Fiscal Year: 2007..Together, these experiments will characterize a novel pathway of integrin cooperation with TF of relevance for endothelial cell migration. ..
- NEW GENETIC MODELS FOR TISSUE FACTOR SIGNALINGWolfram Ruf; Fiscal Year: 2007..Identification of such markers will facilitate the quantitation of coagulation signaling in vivo and thus advance clinical efforts to design therapeutic intervention in coagulant cell signaling pathways . ..
- TARGETING SIGNALING OF THE TISSUE FACTOR PATHWAYWolfram Ruf; Fiscal Year: 2007..abstract_text> ..
- Functional Cooperation of Tissue Factor and IntegrinsWolfram Ruf; Fiscal Year: 2010..Because a prototypic inhibitor of this pathway is effective to attenuate tumor growth in preclinical models, the proposed basic mechanistic studies facilitate a rational translation of this novel concept into the clinic. ..
- TF Signaling in Systemic InflammationWolfram Ruf; Fiscal Year: 2010....
- SIGNALING OF TISSUE FACTOR IN CELL ADHESIONWolfram Ruf; Fiscal Year: 2001..In the long term, elucidation of the molecular details of TF signaling will guide novel therapeutic approaches that intervene with functions of TF in metastasis and angiogenesis. ..
- ALLOSTERIC REGULATION OF FACTOR VII FUNCTIONWolfram Ruf; Fiscal Year: 2002..The detailed insight into allosteric regulation and determinants of substrate specificity in VIIa may provide novel strategies for therapeutic intervention in inflammatory and thrombotic diseases. ..
- Structural Basis of Protease Signaling by VllaWolfram Ruf; Fiscal Year: 2003..abstract_text> ..
- PAR SIGNALING AND PROTECTIVE PATHWAYS IN INFLAMMATION AND SEPSISWolfram Ruf; Fiscal Year: 2010..3. ..