Research Topics
| JOHN REEVESummaryAffiliation: The Ohio State University Country: USA Publications
Research Grants
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Detail Information
Publications
Archaeal chromatin proteins: different structures but common function?Kathleen Sandman
Department of Microbiology, Ohio State University, 484W 12th Avenue, Columbus, OH 43210, USA
Curr Opin Microbiol 8:656-61. 2005..Although their structures and complexes with DNA have no similarities, their functions probably overlap as mutants that lack single chromatin proteins are viable...- Both DNA and histone fold sequences contribute to archaeal nucleosome stabilityKathryn A Bailey
Department of Microbiology, Ohio State University, Columbus, Ohio 43210, USA
J Biol Chem 277:9293-301. 2002.... - Molecular identification of bacteria and Eukarya inhabiting an Antarctic cryoconite holeBrent C Christner
Department of Microbiology, Ohio State University, Columbus, OH 43210-1292, USA
Extremophiles 7:177-83. 2003..This cryoconite hole community was therefore most likely seeded by particulates from these local environments. Cryoconite holes may serve as biological refuges that, on glacial melting, can repopulate the local environments... - Archaeal histones: structures, stability and DNA bindingJ N Reeve
Department of Microbiology, Ohio State University, Columbus, OH 43210, U S A
Biochem Soc Trans 32:227-30. 2004..The histone-fold residues that stabilize dimer-dimer interactions within an archaeal histone core contribute to determining archaeal histone-DNA affinity... - Archaeal chromatin and transcriptionJohn N Reeve
Department of Microbiology, Ohio State University, Columbus, OH 43210 1292, USA
Mol Microbiol 48:587-98. 2003..However, this seems unlikely as it might limit repressor competition at this site to only the first round of transcription initiation... 
Spontaneous trpY mutants and mutational analysis of the TrpY archaeal transcription regulatorKathleen Sandman
Department of Microbiology, Ohio State University, Columbus, OH 43210 1292, USA
J Bacteriol 189:4338-42. 2007..In vivo and in vitro studies revealed that DNA binding was sufficient for TrpY repression of trpY transcription but that TrpY must bind DNA and tryptophan to assemble a complex that represses trpEGCFBAD...- Archaeal RNA polymerase is sensitive to intrinsic termination directed by transcribed and remote sequencesThomas J Santangelo
Department of Microbiology, The Ohio State University, Columbus, OH 43210, USA
J Mol Biol 355:196-210. 2006.... - Archaeal histones and the origin of the histone foldKathleen Sandman
Department of Microbiology, Ohio State University, Columbus, OH 43210, USA
Curr Opin Microbiol 9:520-5. 2006..Interpretation of these structural variations offers clues to the steps that might have occurred during the evolution and specialization of eukaryotic core histones... - TFB1 or TFB2 is sufficient for Thermococcus kodakaraensis viability and for basal transcription in vitroThomas J Santangelo
Department of Microbiology, Ohio State University, Columbus, OH 43210, USA
J Mol Biol 367:344-57. 2007.... - Shuttle vector expression in Thermococcus kodakaraensis: contributions of cis elements to protein synthesis in a hyperthermophilic archaeonThomas J Santangelo
Department of Microbiology, Ohio State University, Columbus, OH 43210 1292, USA
Appl Environ Microbiol 74:3099-104. 2008..kodakaraensis cell lysates by Ni(2+) binding and imidazole elution... 
Archaeal chromatin proteins histone HMtB and Alba have lost DNA-binding ability in laboratory strains of Methanothermobacter thermautotrophicusKathleen Sandman
Department of Microbiology, Ohio State University, Columbus, OH 43210, USA
Extremophiles 12:811-7. 2008..The loss of DNA binding by HMtB(I19) does not therefore prevent HMtB from participating in DNA interactions as one partner of an archaeal histone heterodimer...- Histones in crenarchaeaKathleen Sandman
Department of Microbiology, Ohio State University, Columbus, OH 43210, USA
J Bacteriol 187:5482-5. 2005..The discovery of histones in Crenarchaea supports the argument that histones evolved before the divergence of Archaea and Eukarya... - Deletion of switch 3 results in an archaeal RNA polymerase that is defective in transcript elongationThomas J Santangelo
Department of Microbiology, Ohio State University, Columbus, OH 43210, USA
J Biol Chem 285:23908-15. 2010..The close structural homology of archaeal and eukaryotic RNAPs would predict that eukaryotic Switch 3 loops likely conform to the archaeal rather than bacterial functional paradigm... - Transcription by Methanothermobacter thermautotrophicus RNA polymerase in vitro releases archaeal transcription factor B but not TATA-box binding protein from the template DNAYunwei Xie
Department of Microbiology, Ohio State University, Columbus, OH 43210-1292, USA
J Bacteriol 186:6306-10. 2004..Regulation of archaeal transcription initiation by a repressor competition with TBP for TATA-box region binding must accommodate this observation... - Archaeal histone tetramerization determines DNA affinity and the direction of DNA supercoilingFrederic Marc
Department of Microbiology, Ohio State University, Columbus, OH 43210, USA
J Biol Chem 277:30879-86. 2002..Therefore, by regulating the assembly of different archaeal histone dimers into tetramers that have different sequence affinities, the assembly of archaeal histone-DNA complexes could be localized and used to regulate gene expression... - Transcription by an archaeal RNA polymerase is slowed but not blocked by an archaeal nucleosomeYunwei Xie
Department of Microbiology, Ohio State University, Columbus, Ohio 43210-1292, USA
J Bacteriol 186:3492-8. 2004..This inhibited transcription presumably by preventing archaeal TATA-box binding protein, general transcription factor TFB, and RNAP access and thus inhibiting transcription initiation... - TrpY regulation of trpB2 transcription in Methanothermobacter thermautotrophicusElizabeth A Karr
Department of Microbiology, Ohio State University, Columbus, OH 43210 1292, USA
J Bacteriol 190:2637-41. 2008..Inhibitory complex formation is prevented by insertions within the regulatory region and by a G149R substitution in TrpY, even though TrpY(G149R) retains both TRP box DNA- and tryptophan-binding abilities... - Polarity in archaeal operon transcription in Thermococcus kodakaraensisThomas J Santangelo
Department of Microbiology, Ohio State University, Columbus, OH 43210 1292, USA
J Bacteriol 190:2244-8. 2008..Following the introduction of nonsense codons into promoter-proximal genes, polarity in operon expression in this archaeon has been established by both microarray hybridization assays and a reporter gene expression system... - Archaeal intrinsic transcription termination in vivoThomas J Santangelo
Department of Microbiology, Ohio State University, Columbus, OH 43210, USA
J Bacteriol 191:7102-8. 2009.... - In vitro transcription assays using components from Methanothermobacter thermautotrophicusYunwei Xie
Department of Microbiology, Ohio State University, Columbus, Ohio 43210, USA
Methods Enzymol 370:66-72. 2003 - The Fur iron regulator-like protein is cryptic in the hyperthermophilic archaeon Thermococcus kodakaraensisHélène Louvel
Department of Microbiology, Ohio State University, Columbus, OH, USA
FEMS Microbiol Lett 295:117-28. 2009..kodakaraensis diphtheria toxin regulator (DtxR) homolog may control the expression of the major iron acquisition effectors, while its inactivation enabled higher resistance to iron deficiency... - Conserved eukaryotic histone-fold residues substituted into an archaeal histone increase DNA affinity but reduce complex flexibilityDivya J Soares
Department of Microbiology, The Ohio State University, Columbus, Ohio 43210-1292, USA
J Bacteriol 185:3453-7. 2003..However, these complexes no longer facilitated the circularization of short DNA molecules and had lost the flexibility to wrap DNA alternatively in either a negative or positive supercoil... - Thermococcus kodakarensis genetics: TK1827-encoded beta-glycosidase, new positive-selection protocol, and targeted and repetitive deletion technologyThomas J Santangelo
Department of Microbiology, Ohio State University, Columbus, OH 43210, USA
Appl Environ Microbiol 76:1044-52. 2010..Transformants can therefore be selected rapidly, and replicating plasmids can be maintained in this strain growing in rich medium by complementation of the TK0149 deletion... - Bacterial recovery from ancient glacial iceBrent C Christner
Department of Microbiology, Ohio State University, Columbus, OH 43210-1292, USA
Environ Microbiol 5:433-6. 2003..These results demonstrate that bacteria can be recovered from water ice that has frozen for time periods relevant to biological survival through terrestrial ice ages or during interplanetary transport... - Regulation of tryptophan operon expression in the archaeon Methanothermobacter thermautotrophicusYunwei Xie
Department of Microbiology, Ohio State University, Columbus, Ohio 43210-1292, USA
J Bacteriol 187:6419-29. 2005.... - Deletion of the archaeal histone in Methanosarcina mazei Gö1 results in reduced growth and genomic transcriptionKatrin Weidenbach
Institut fur Allgemeine Mikrobiologie, Christian Albrechts Universitat zu Kiel, Am Botanischen Garten 1 9, 24118 Kiel, Germany
Mol Microbiol 67:662-71. 2008..mazei MM1825::pac cells. Complementary synthesis of HMm from a plasmid transformed into M. mazei MM1825::pac restored wild-type growth and transcript levels... - The genome of M. acetivorans reveals extensive metabolic and physiological diversityJames E Galagan
Whitehead Institute Center for Genome Research, Cambridge, Massachusetts 02141, USA
Genome Res 12:532-42. 2002..acetivorans a powerful model organism for the study of archaeal biology. [Sequence, data, annotations and analyses are available at http://www-genome.wi.mit.edu/.].. - Archaeal minichromosome maintenance (MCM) helicase can unwind DNA bound by archaeal histones and transcription factorsJae Ho Shin
Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland 20850, USA
J Biol Chem 282:4908-15. 2007..In contrast, the minichromosome maintenance helicase was unable to unwind DNA bound by this archaeal RNA polymerase in a stalled transcript-elongating complex... - Biology, biochemistry and the molecular machinery of ArchaeaJohn N Reeve
Curr Opin Microbiol 8:627-9. 2005 - Transcription and translation are coupled in ArchaeaSarah L French
Mol Biol Evol 24:893-5. 2007..These complexes are consistent with transcription and translation being coupled in this Archaeon, with translation of transcripts being initiated before the transcript is complete... 
Use of a restriction enzyme-digested PCR product as substrate for helicase assaysJae-Ho Shin
University of Maryland Biotechnology Institute, Center for Advanced Research in Biotechnology 9600 Gudelsky Drive, Rockville, MD 20850, USA
Nucleic Acids Res 33:e8. 2005..Here, a PCR-based method to generate a substrate for a helicase assay is described, and its application for several archaeal, bacterial and viral enzymes is demonstrated...
Research Grants
- HISTONES AND NUCLEOSOMES IN ARCHAEA (ARCHAEBACTERIA)JOHN REEVE; Fiscal Year: 2002....
- Histones and Nucleosomes in ArchaeaJOHN REEVE; Fiscal Year: 2007..abstract_text> ..