JOHN REEVE

Summary

Affiliation: The Ohio State University
Country: USA

Publications

  1. ncbi request reprint Archaeal chromatin proteins: different structures but common function?
    Kathleen Sandman
    Department of Microbiology, Ohio State University, 484W 12th Avenue, Columbus, OH 43210, USA
    Curr Opin Microbiol 8:656-61. 2005
  2. ncbi request reprint Both DNA and histone fold sequences contribute to archaeal nucleosome stability
    Kathryn A Bailey
    Department of Microbiology, Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 277:9293-301. 2002
  3. ncbi request reprint Molecular identification of bacteria and Eukarya inhabiting an Antarctic cryoconite hole
    Brent C Christner
    Department of Microbiology, Ohio State University, Columbus, OH 43210 1292, USA
    Extremophiles 7:177-83. 2003
  4. pmc Archaeal nucleosome positioning in vivo and in vitro is directed by primary sequence motifs
    Narasimharao Nalabothula
    Department of Molecular and Cellular Biochemistry, College of Medicine, University of Kentucky, Lexington, KY 40536, USA
    BMC Genomics 14:391. 2013
  5. ncbi request reprint Archaeal chromatin and transcription
    John N Reeve
    Department of Microbiology, Ohio State University, Columbus, OH 43210 1292, USA
    Mol Microbiol 48:587-98. 2003
  6. ncbi request reprint Archaeal histones: structures, stability and DNA binding
    J N Reeve
    Department of Microbiology, Ohio State University, Columbus, OH 43210, U S A
    Biochem Soc Trans 32:227-30. 2004
  7. pmc Spontaneous trpY mutants and mutational analysis of the TrpY archaeal transcription regulator
    Kathleen Sandman
    Department of Microbiology, Ohio State University, Columbus, OH 43210 1292, USA
    J Bacteriol 189:4338-42. 2007
  8. ncbi request reprint Archaeal RNA polymerase is sensitive to intrinsic termination directed by transcribed and remote sequences
    Thomas J Santangelo
    Department of Microbiology, The Ohio State University, Columbus, OH 43210, USA
    J Mol Biol 355:196-210. 2006
  9. ncbi request reprint Archaeal histone tetramerization determines DNA affinity and the direction of DNA supercoiling
    Frederic Marc
    Department of Microbiology, Ohio State University, Columbus, OH 43210, USA
    J Biol Chem 277:30879-86. 2002
  10. pmc TFB1 or TFB2 is sufficient for Thermococcus kodakaraensis viability and for basal transcription in vitro
    Thomas J Santangelo
    Department of Microbiology, Ohio State University, Columbus, OH 43210, USA
    J Mol Biol 367:344-57. 2007

Research Grants

  1. HISTONES AND NUCLEOSOMES IN ARCHAEA (ARCHAEBACTERIA)
    JOHN REEVE; Fiscal Year: 2002
  2. Histones and Nucleosomes in Archaea
    JOHN REEVE; Fiscal Year: 2007

Collaborators

Detail Information

Publications33

  1. ncbi request reprint Archaeal chromatin proteins: different structures but common function?
    Kathleen Sandman
    Department of Microbiology, Ohio State University, 484W 12th Avenue, Columbus, OH 43210, USA
    Curr Opin Microbiol 8:656-61. 2005
    ..Although their structures and complexes with DNA have no similarities, their functions probably overlap as mutants that lack single chromatin proteins are viable...
  2. ncbi request reprint Both DNA and histone fold sequences contribute to archaeal nucleosome stability
    Kathryn A Bailey
    Department of Microbiology, Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 277:9293-301. 2002
    ....
  3. ncbi request reprint Molecular identification of bacteria and Eukarya inhabiting an Antarctic cryoconite hole
    Brent C Christner
    Department of Microbiology, Ohio State University, Columbus, OH 43210 1292, USA
    Extremophiles 7:177-83. 2003
    ..This cryoconite hole community was therefore most likely seeded by particulates from these local environments. Cryoconite holes may serve as biological refuges that, on glacial melting, can repopulate the local environments...
  4. pmc Archaeal nucleosome positioning in vivo and in vitro is directed by primary sequence motifs
    Narasimharao Nalabothula
    Department of Molecular and Cellular Biochemistry, College of Medicine, University of Kentucky, Lexington, KY 40536, USA
    BMC Genomics 14:391. 2013
    ..The experiments reported were undertaken to determine if archaeal histone assembly conforms to the nucleosome positioning code...
  5. ncbi request reprint Archaeal chromatin and transcription
    John N Reeve
    Department of Microbiology, Ohio State University, Columbus, OH 43210 1292, USA
    Mol Microbiol 48:587-98. 2003
    ..However, this seems unlikely as it might limit repressor competition at this site to only the first round of transcription initiation...
  6. ncbi request reprint Archaeal histones: structures, stability and DNA binding
    J N Reeve
    Department of Microbiology, Ohio State University, Columbus, OH 43210, U S A
    Biochem Soc Trans 32:227-30. 2004
    ..The histone-fold residues that stabilize dimer-dimer interactions within an archaeal histone core contribute to determining archaeal histone-DNA affinity...
  7. pmc Spontaneous trpY mutants and mutational analysis of the TrpY archaeal transcription regulator
    Kathleen Sandman
    Department of Microbiology, Ohio State University, Columbus, OH 43210 1292, USA
    J Bacteriol 189:4338-42. 2007
    ..In vivo and in vitro studies revealed that DNA binding was sufficient for TrpY repression of trpY transcription but that TrpY must bind DNA and tryptophan to assemble a complex that represses trpEGCFBAD...
  8. ncbi request reprint Archaeal RNA polymerase is sensitive to intrinsic termination directed by transcribed and remote sequences
    Thomas J Santangelo
    Department of Microbiology, The Ohio State University, Columbus, OH 43210, USA
    J Mol Biol 355:196-210. 2006
    ....
  9. ncbi request reprint Archaeal histone tetramerization determines DNA affinity and the direction of DNA supercoiling
    Frederic Marc
    Department of Microbiology, Ohio State University, Columbus, OH 43210, USA
    J Biol Chem 277:30879-86. 2002
    ..Therefore, by regulating the assembly of different archaeal histone dimers into tetramers that have different sequence affinities, the assembly of archaeal histone-DNA complexes could be localized and used to regulate gene expression...
  10. pmc TFB1 or TFB2 is sufficient for Thermococcus kodakaraensis viability and for basal transcription in vitro
    Thomas J Santangelo
    Department of Microbiology, Ohio State University, Columbus, OH 43210, USA
    J Mol Biol 367:344-57. 2007
    ....
  11. pmc Shuttle vector expression in Thermococcus kodakaraensis: contributions of cis elements to protein synthesis in a hyperthermophilic archaeon
    Thomas J Santangelo
    Department of Microbiology, Ohio State University, Columbus, OH 43210 1292, USA
    Appl Environ Microbiol 74:3099-104. 2008
    ..kodakaraensis cell lysates by Ni(2+) binding and imidazole elution...
  12. pmc Archaeal chromatin proteins histone HMtB and Alba have lost DNA-binding ability in laboratory strains of Methanothermobacter thermautotrophicus
    Kathleen Sandman
    Department of Microbiology, Ohio State University, Columbus, OH 43210, USA
    Extremophiles 12:811-7. 2008
    ..The loss of DNA binding by HMtB(I19) does not therefore prevent HMtB from participating in DNA interactions as one partner of an archaeal histone heterodimer...
  13. ncbi request reprint Archaeal histones and the origin of the histone fold
    Kathleen Sandman
    Department of Microbiology, Ohio State University, Columbus, OH 43210, USA
    Curr Opin Microbiol 9:520-5. 2006
    ..Interpretation of these structural variations offers clues to the steps that might have occurred during the evolution and specialization of eukaryotic core histones...
  14. pmc Transcription by Methanothermobacter thermautotrophicus RNA polymerase in vitro releases archaeal transcription factor B but not TATA-box binding protein from the template DNA
    Yunwei Xie
    Department of Microbiology, Ohio State University, Columbus, OH 43210 1292, USA
    J Bacteriol 186:6306-10. 2004
    ..Regulation of archaeal transcription initiation by a repressor competition with TBP for TATA-box region binding must accommodate this observation...
  15. pmc Histones in crenarchaea
    L ubomíra Cubonová
    Department of Microbiology, Ohio State University, Columbus, OH 43210, USA
    J Bacteriol 187:5482-5. 2005
    ..The discovery of histones in Crenarchaea supports the argument that histones evolved before the divergence of Archaea and Eukarya...
  16. pmc Transcription by an archaeal RNA polymerase is slowed but not blocked by an archaeal nucleosome
    Yunwei Xie
    Department of Microbiology, Ohio State University, Columbus, Ohio 43210 1292, USA
    J Bacteriol 186:3492-8. 2004
    ..This inhibited transcription presumably by preventing archaeal TATA-box binding protein, general transcription factor TFB, and RNAP access and thus inhibiting transcription initiation...
  17. pmc Deletion of switch 3 results in an archaeal RNA polymerase that is defective in transcript elongation
    Thomas J Santangelo
    Department of Microbiology, Ohio State University, Columbus, OH 43210, USA
    J Biol Chem 285:23908-15. 2010
    ..The close structural homology of archaeal and eukaryotic RNAPs would predict that eukaryotic Switch 3 loops likely conform to the archaeal rather than bacterial functional paradigm...
  18. pmc TrpY regulation of trpB2 transcription in Methanothermobacter thermautotrophicus
    Elizabeth A Karr
    Department of Microbiology, Ohio State University, Columbus, OH 43210 1292, USA
    J Bacteriol 190:2637-41. 2008
    ..Inhibitory complex formation is prevented by insertions within the regulatory region and by a G149R substitution in TrpY, even though TrpY(G149R) retains both TRP box DNA- and tryptophan-binding abilities...
  19. pmc Polarity in archaeal operon transcription in Thermococcus kodakaraensis
    Thomas J Santangelo
    Department of Microbiology, Ohio State University, Columbus, OH 43210 1292, USA
    J Bacteriol 190:2244-8. 2008
    ..Following the introduction of nonsense codons into promoter-proximal genes, polarity in operon expression in this archaeon has been established by both microarray hybridization assays and a reporter gene expression system...
  20. pmc Archaeal intrinsic transcription termination in vivo
    Thomas J Santangelo
    Department of Microbiology, Ohio State University, Columbus, OH 43210, USA
    J Bacteriol 191:7102-8. 2009
    ....
  21. ncbi request reprint The Fur iron regulator-like protein is cryptic in the hyperthermophilic archaeon Thermococcus kodakaraensis
    Hélène Louvel
    Department of Microbiology, Ohio State University, Columbus, OH, USA
    FEMS Microbiol Lett 295:117-28. 2009
    ..kodakaraensis diphtheria toxin regulator (DtxR) homolog may control the expression of the major iron acquisition effectors, while its inactivation enabled higher resistance to iron deficiency...
  22. pmc Conserved eukaryotic histone-fold residues substituted into an archaeal histone increase DNA affinity but reduce complex flexibility
    Divya J Soares
    Department of Microbiology, The Ohio State University, Columbus, Ohio 43210 1292, USA
    J Bacteriol 185:3453-7. 2003
    ..However, these complexes no longer facilitated the circularization of short DNA molecules and had lost the flexibility to wrap DNA alternatively in either a negative or positive supercoil...
  23. ncbi request reprint In vitro transcription assays using components from Methanothermobacter thermautotrophicus
    Yunwei Xie
    Department of Microbiology, Ohio State University, Columbus, Ohio 43210, USA
    Methods Enzymol 370:66-72. 2003
  24. pmc Thermococcus kodakarensis genetics: TK1827-encoded beta-glycosidase, new positive-selection protocol, and targeted and repetitive deletion technology
    Thomas J Santangelo
    Department of Microbiology, Ohio State University, Columbus, OH 43210, USA
    Appl Environ Microbiol 76:1044-52. 2010
    ..Transformants can therefore be selected rapidly, and replicating plasmids can be maintained in this strain growing in rich medium by complementation of the TK0149 deletion...
  25. ncbi request reprint Bacterial recovery from ancient glacial ice
    Brent C Christner
    Department of Microbiology, Ohio State University, Columbus, OH 43210 1292, USA
    Environ Microbiol 5:433-6. 2003
    ..These results demonstrate that bacteria can be recovered from water ice that has frozen for time periods relevant to biological survival through terrestrial ice ages or during interplanetary transport...
  26. pmc Regulation of tryptophan operon expression in the archaeon Methanothermobacter thermautotrophicus
    Yunwei Xie
    Department of Microbiology, Ohio State University, Columbus, Ohio 43210 1292, USA
    J Bacteriol 187:6419-29. 2005
    ....
  27. ncbi request reprint Deletion of the archaeal histone in Methanosarcina mazei Gö1 results in reduced growth and genomic transcription
    Katrin Weidenbach
    Institut fur Allgemeine Mikrobiologie, Christian Albrechts Universitat zu Kiel, Am Botanischen Garten 1 9, 24118 Kiel, Germany
    Mol Microbiol 67:662-71. 2008
    ..mazei MM1825::pac cells. Complementary synthesis of HMm from a plasmid transformed into M. mazei MM1825::pac restored wild-type growth and transcript levels...
  28. ncbi request reprint Archaeal minichromosome maintenance (MCM) helicase can unwind DNA bound by archaeal histones and transcription factors
    Jae Ho Shin
    Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland 20850, USA
    J Biol Chem 282:4908-15. 2007
    ..In contrast, the minichromosome maintenance helicase was unable to unwind DNA bound by this archaeal RNA polymerase in a stalled transcript-elongating complex...
  29. ncbi request reprint Biology, biochemistry and the molecular machinery of Archaea
    John N Reeve
    Curr Opin Microbiol 8:627-9. 2005
  30. pmc The genome of M. acetivorans reveals extensive metabolic and physiological diversity
    James E Galagan
    Whitehead Institute Center for Genome Research, Cambridge, Massachusetts 02141, USA
    Genome Res 12:532-42. 2002
    ..acetivorans a powerful model organism for the study of archaeal biology. [Sequence, data, annotations and analyses are available at http://www-genome.wi.mit.edu/.]..
  31. pmc Use of a restriction enzyme-digested PCR product as substrate for helicase assays
    Jae Ho Shin
    University of Maryland Biotechnology Institute, Center for Advanced Research in Biotechnology 9600 Gudelsky Drive, Rockville, MD 20850, USA
    Nucleic Acids Res 33:e8. 2005
    ..Here, a PCR-based method to generate a substrate for a helicase assay is described, and its application for several archaeal, bacterial and viral enzymes is demonstrated...
  32. ncbi request reprint Transcription and translation are coupled in Archaea
    Sarah L French
    Mol Biol Evol 24:893-5. 2007
    ..These complexes are consistent with transcription and translation being coupled in this Archaeon, with translation of transcripts being initiated before the transcript is complete...

Research Grants11

  1. HISTONES AND NUCLEOSOMES IN ARCHAEA (ARCHAEBACTERIA)
    JOHN REEVE; Fiscal Year: 2002
    ....
  2. Histones and Nucleosomes in Archaea
    JOHN REEVE; Fiscal Year: 2007
    ..abstract_text> ..