J C Reed

Summary

Affiliation: The Burnham Institute
Country: USA

Publications

  1. ncbi Bc1-2, Raf-1 and mitochondrial regulation of apoptosis
    H G Wang
    Burnham Institute, La Jolla, CA 92037, USA
    Biofactors 8:13-6. 1998
  2. ncbi Bifunctional apoptosis inhibitor (BAR) protects neurons from diverse cell death pathways
    W Roth
    The Burnham Institute, 10901 N Torrey Pines Road, La Jolla, CA 92037, USA
    Cell Death Differ 10:1178-87. 2003
  3. ncbi Drug insight: cancer therapy strategies based on restoration of endogenous cell death mechanisms
    John C Reed
    Burnham Institute for Medical Research, La Jolla, CA 92037, USA
    Nat Clin Pract Oncol 3:388-98. 2006
  4. ncbi Cancer immunotherapy targeting survivin: commentary re: V. Pisarev et al., full-length dominant-negative survivin for cancer immunotherapy. Clin. Cancer Res., 9:6523-6533, 2003
    John C Reed
    The Burnham Institute, La Jolla, California 92037, USA
    Clin Cancer Res 9:6310-5. 2003
  5. ncbi Apoptosis mechanisms: implications for cancer drug discovery
    John C Reed
    The Burnham Institute, La Jolla, California 92037, USA
    Oncology (Williston Park) 18:11-20. 2004
  6. ncbi Bcl-2-family proteins and hematologic malignancies: history and future prospects
    John C Reed
    Burnham Institute for Medical Research, La Jolla, CA 92037, USA
    Blood 111:3322-30. 2008
  7. ncbi Apoptosis-based therapies for hematologic malignancies
    John C Reed
    Burnham Institute, 10901 N Torrey Pines Rd, La Jolla, CA 92037, USA
    Blood 106:408-18. 2005
  8. ncbi cIAP1 Localizes to the nuclear compartment and modulates the cell cycle
    Temesgen Samuel
    Burnham Institute, La Jolla, CA 92037, USA
    Cancer Res 65:210-8. 2005
  9. ncbi Proapoptotic multidomain Bcl-2/Bax-family proteins: mechanisms, physiological roles, and therapeutic opportunities
    J C Reed
    Burnham Institute for Medical Research, La Jolla, CA 92037, USA
    Cell Death Differ 13:1378-86. 2006
  10. ncbi A diverse family of proteins containing tumor necrosis factor receptor-associated factor domains
    J M Zapata
    Burnham Institute, La Jolla, California 92037, USA
    J Biol Chem 276:24242-52. 2001

Research Grants

  1. MITOCHONDRIA AND APOPTOSIS
    John Reed; Fiscal Year: 2007
  2. Yeast-based HTS Assay Technologies for Proteases
    John Reed; Fiscal Year: 2009
  3. Chemical Antagonists of IAP-Family Anti-Apoptotic Proteins
    John Reed; Fiscal Year: 2007
  4. SIGNAL TRANSDUCTION AND CELL DEATH REGULATION
    John Reed; Fiscal Year: 2007
  5. Apoptosis-Based Cancer Drug Discovery
    John Reed; Fiscal Year: 2007
  6. Mechanisms of the HBX-interacting protein (HBXIP)
    John Reed; Fiscal Year: 2007
  7. PAAD-Family Proteins and Host Defense Mechanisms
    John Reed; Fiscal Year: 2007
  8. Chemical Inhibitors of ER Stress
    John Reed; Fiscal Year: 2007
  9. NLR Family Proteins: Mechanisms and Regulation
    John Reed; Fiscal Year: 2009
  10. BI-1, A Regulator of ER-Stress Pathways Linked to Apoptosis
    John Reed; Fiscal Year: 2009

Collaborators

Detail Information

Publications162 found, 100 shown here

  1. ncbi Bc1-2, Raf-1 and mitochondrial regulation of apoptosis
    H G Wang
    Burnham Institute, La Jolla, CA 92037, USA
    Biofactors 8:13-6. 1998
    ..The findings suggest that Bcl-2 targets Raf-1 to mitochondria, allowing this kinase to contribute to cellular survival by phosphorylating BAD or possibly other protein substrates in the vicinity of Bcl-2...
  2. ncbi Bifunctional apoptosis inhibitor (BAR) protects neurons from diverse cell death pathways
    W Roth
    The Burnham Institute, 10901 N Torrey Pines Road, La Jolla, CA 92037, USA
    Cell Death Differ 10:1178-87. 2003
    ..Taken together, the expression pattern and functional data suggest that the BAR protein is involved in the regulation of neuronal survival...
  3. ncbi Drug insight: cancer therapy strategies based on restoration of endogenous cell death mechanisms
    John C Reed
    Burnham Institute for Medical Research, La Jolla, CA 92037, USA
    Nat Clin Pract Oncol 3:388-98. 2006
    ..Several of the current strategies based on targeting core components of the cell death machinery for cancer therapy are reviewed here, and a summary of progress toward clinical applications is provided...
  4. ncbi Cancer immunotherapy targeting survivin: commentary re: V. Pisarev et al., full-length dominant-negative survivin for cancer immunotherapy. Clin. Cancer Res., 9:6523-6533, 2003
    John C Reed
    The Burnham Institute, La Jolla, California 92037, USA
    Clin Cancer Res 9:6310-5. 2003
  5. ncbi Apoptosis mechanisms: implications for cancer drug discovery
    John C Reed
    The Burnham Institute, La Jolla, California 92037, USA
    Oncology (Williston Park) 18:11-20. 2004
    ..Apoptosis-regulating genes are also beginning to find utility as targets for antisense oligonucleotides...
  6. ncbi Bcl-2-family proteins and hematologic malignancies: history and future prospects
    John C Reed
    Burnham Institute for Medical Research, La Jolla, CA 92037, USA
    Blood 111:3322-30. 2008
    ..Experimental therapies targeting Bcl-2 family mRNAs or proteins are currently in clinical testing, raising hopes that a new class of anticancer drugs may be near...
  7. ncbi Apoptosis-based therapies for hematologic malignancies
    John C Reed
    Burnham Institute, 10901 N Torrey Pines Rd, La Jolla, CA 92037, USA
    Blood 106:408-18. 2005
    ....
  8. ncbi cIAP1 Localizes to the nuclear compartment and modulates the cell cycle
    Temesgen Samuel
    Burnham Institute, La Jolla, CA 92037, USA
    Cancer Res 65:210-8. 2005
    ..Our findings demonstrate a role for overexpressed cIAP1 in genetic instability, possibly by interfering with mitotic functions of Survivin. These findings may have important implications for cancers in which cIAP1 overexpression occurs...
  9. ncbi Proapoptotic multidomain Bcl-2/Bax-family proteins: mechanisms, physiological roles, and therapeutic opportunities
    J C Reed
    Burnham Institute for Medical Research, La Jolla, CA 92037, USA
    Cell Death Differ 13:1378-86. 2006
    ....
  10. ncbi A diverse family of proteins containing tumor necrosis factor receptor-associated factor domains
    J M Zapata
    Burnham Institute, La Jolla, California 92037, USA
    J Biol Chem 276:24242-52. 2001
    ..We propose the moniker TEFs (TD-encompassing factors) for this large family of proteins...
  11. ncbi Regulation of Nur77 nuclear export by c-Jun N-terminal kinase and Akt
    Y H Han
    Burnham Institute for Medical Research, Cancer Center, La Jolla, CA 92037, USA
    Oncogene 25:2974-86. 2006
    ..Together, our results demonstrate that both activation of JNK and inhibition of Akt play a role in translocation of Nur77 from the nucleus to the cytoplasm...
  12. ncbi Microtubule-targeting drugs induce bcl-2 phosphorylation and association with Pin1
    N Pathan
    The Burnham Institute, 10901 N Torrey Pines Rd, La Jolla, CA 92037, USA
    Neoplasia 3:550-9. 2001
    ....
  13. ncbi TRAF1 is a substrate of caspases activated during tumor necrosis factor receptor-alpha-induced apoptosis
    E Leo
    Burnham Institute, La Jolla, California 92037, USA
    J Biol Chem 276:8087-93. 2001
    ..These data identify TRAF1 as a specific target of caspases activated during TNF- and Fas-induced apoptosis and illustrate differences in the repertoire of protease substrates cleaved during activation of different apoptotic pathways...
  14. ncbi TUCAN, an antiapoptotic caspase-associated recruitment domain family protein overexpressed in cancer
    N Pathan
    Burnham Institute, La Jolla, California 92037, USA
    J Biol Chem 276:32220-9. 2001
    ..Thus, TUCAN represents a new member of the CARD family that selectively suppresses apoptosis induced via the mitochondrial pathway for caspase activation...
  15. ncbi Bcl-B, a novel Bcl-2 family member that differentially binds and regulates Bax and Bak
    N Ke
    Burnham Institute, La Jolla, California 92037, USA
    J Biol Chem 276:12481-4. 2001
    ..Bcl-B thus displays a unique pattern of selectivity for binding and regulating the function of other members of the Bcl-2 family...
  16. ncbi CLAN, a novel human CED-4-like gene
    J S Damiano
    The Burnham Institute, La Jolla, California 92037, USA
    Genomics 75:77-83. 2001
    ..The CARD of the CLAN proteins binds a number of other CARD-containing proteins including caspase-1, BCL10, NOD2, and NAC. Once their physiologic functions are uncovered, CLAN proteins may prove to be valuable therapeutic targets...
  17. ncbi The c-IAP-1 and c-IAP-2 proteins are direct inhibitors of specific caspases
    N Roy
    The Burnham Institute, Program on Apoptosis and Cell Death Research, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
    EMBO J 16:6914-25. 1997
    ..Taken together, these findings suggest that c-IAP-1 and c-IAP-2 function similarly to XIAP by inhibiting the distal cell death proteases, caspases-3 and -7, whereas NAIP presumably inhibits apoptosis via other targets...
  18. ncbi Structure-function analysis of Bag1 proteins. Effects on androgen receptor transcriptional activity
    D A Knee
    Burnham Institute, La Jolla, California 92037, USA
    J Biol Chem 276:12718-24. 2001
    ..Thus, both the unique NH(2)-terminal domain and the COOH-terminal Hsc70-binding domain of Bag1L are simultaneously required for its function as an AR regulator, whereas the conserved ubiquitin-like domain is expendable...
  19. ncbi BAR: An apoptosis regulator at the intersection of caspases and Bcl-2 family proteins
    H Zhang
    Program on Apoptosis and Cell Death Regulation, The Burnham Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 97:2597-602. 2000
    ..The BAR protein is anchored in intracellular membranes where Bcl-2 resides. BAR therefore may represent a scaffold protein capable of bridging two major apoptosis pathways...
  20. ncbi IAP-family protein survivin inhibits caspase activity and apoptosis induced by Fas (CD95), Bax, caspases, and anticancer drugs
    I Tamm
    The Burnham Institute, La Jolla, California 92037, USA
    Cancer Res 58:5315-20. 1998
    ..Although quantitative differences may exist, these observations suggest commonality in the mechanisms used by IAP-family proteins to suppress apoptosis...
  21. ncbi IAPs block apoptotic events induced by caspase-8 and cytochrome c by direct inhibition of distinct caspases
    Q L Deveraux
    The Burnham Institute, Program on Apoptosis and Cell Death Research, 10901 N Torrey Pines Road, La Jolla, CA 92037, USA
    EMBO J 17:2215-23. 1998
    ..These findings demonstrate that IAPs can suppress different apoptotic pathways by inhibiting distinct caspases and identify pro-caspase-9 as a new target for IAP-mediated inhibition of apoptosis...
  22. ncbi Changes in intramitochondrial and cytosolic pH: early events that modulate caspase activation during apoptosis
    S Matsuyama
    Programme on Apoptosis and Cell Death Regulation, The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Cell Biol 2:318-25. 2000
    ..These findings indicate that mitochondria-mediated alteration of intracellular pH may be an early event that regulates caspase activation in the mitochondrial pathway for apoptosis...
  23. ncbi Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses
    S I Matsuzawa
    The Burnham Institute, 10901 N Torrey Pines Road, La Jolla, CA 92037, USA
    Mol Cell 7:915-26. 2001
    ..Expression of Siah is induced by p53, revealing a way of linking genotoxic injury to destruction of beta-catenin, thus reducing activity of Tcf/LEF transcription factors and contributing to cell cycle arrest...
  24. ncbi Dynamics of expression of apoptosis-regulatory proteins Bid, Bcl-2, Bcl-X, Bax and Bak during development of murine nervous system
    M Krajewska
    The Burnham Institute, 10901 N Torrey Pines Road, La Jolla, CA 92037, USA
    Cell Death Differ 9:145-57. 2002
    ..Among the Bcl-2 family proteins only Bid and Bcl-XL continue to be expressed at high levels in the adult brain...
  25. ncbi Ca2+-induced apoptosis through calcineurin dephosphorylation of BAD
    H G Wang
    The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 284:339-43. 1999
    ..Thus, a Ca2+-inducible mechanism for apoptosis induction operates by regulating BAD phosphorylation and localization in cells...
  26. ncbi Structural analysis of BAG1 cochaperone and its interactions with Hsc70 heat shock protein
    K Briknarová
    The Burnham Institute, La Jolla, California 92037, USA
    Nat Struct Biol 8:349-52. 2001
    ..The results provide a structural basis for understanding the mechanism by which BAG proteins link molecular chaperones and cell signaling pathways...
  27. ncbi Immunohistochemical analysis of in vivo patterns of expression of CPP32 (Caspase-3), a cell death protease
    M Krajewska
    Apoptosis and Cell Death Research Program, The Burnham Institute, La Jolla, California 92037, USA
    Cancer Res 57:1605-13. 1997
    ..These findings establish for the first time the cell type- and differentiation-specific patterns of expression of an interleukin-1beta converting enzyme/CED-3 (Caspase) family protease...
  28. ncbi Release of caspase-9 from mitochondria during neuronal apoptosis and cerebral ischemia
    S Krajewski
    The Burnham Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 96:5752-7. 1999
    ..Loss of mitochondrial barrier function during neuronal damage from ischemia or other insults therefore may play an important role in making certain caspases available to participate in apoptosis...
  29. ncbi Bax inhibitor-1, a mammalian apoptosis suppressor identified by functional screening in yeast
    Q Xu
    Burnham Institute Program on Apoptosis and Cell Death Research La Jolla, California 92037, USA
    Mol Cell 1:337-46. 1998
    ..Conversely, BI-1 antisense induced apoptosis. BI-1 thus represents a new type of regulator of cell death pathways controlled by Bcl-2 and Bax...
  30. ncbi Expression and location of Hsp70/Hsc-binding anti-apoptotic protein BAG-1 and its variants in normal tissues and tumor cell lines
    S Takayama
    The Burnham Institute, La Jolla, California 92037, USA
    Cancer Res 58:3116-31. 1998
    ....
  31. ncbi The carboxyl-terminal lobe of Hsc70 ATPase domain is sufficient for binding to BAG1
    L Brive
    The Burnham Institute, 10901 N Torrey Pines Road, La Jolla, California 92037, USA
    Biochem Biophys Res Commun 289:1099-105. 2001
    ..This result suggests that the stabilizing contacts for docking of BAG1 to Hsc70 are located in the C-terminal lobe of the ATPase domain. These findings provide new insights into the role of co-chaperones as nucleotide exchange factors...
  32. ncbi Regulation of cell death protease caspase-9 by phosphorylation
    M H Cardone
    Program on Apoptosis and Cell Death Research, The Burnham Institute, La Jolla, CA 92037, USA
    Science 282:1318-21. 1998
    ..Mutant pro-Casp9(Ser196Ala) was resistant to Akt-mediated phosphorylation and inhibition in vitro and in cells, resulting in Akt-resistant induction of apoptosis. Thus, caspases can be directly regulated by protein phosphorylation...
  33. ncbi BIRinging chromosomes through cell division--and survivin' the experience
    J C Reed
    The Burnham Institute, La Jolla, California 92037, USA
    Cell 102:545-8. 2000
  34. ncbi A GTP-binding adapter protein couples TRAIL receptors to apoptosis-inducing proteins
    T Miyazaki
    The Burnham Institute, 10901 N Torrey Pines Rd, La Jolla, CA 92037, USA
    Nat Immunol 2:493-500. 2001
    ..Elucidation of this mechanism suggests GTP-binding proteins as potential targets for pharmacological intervention in TRAIL-induced apoptosis...
  35. ncbi Microtubule-targeting drugs induce Bcl-2 phosphorylation and association with Pin1
    N Pathan
    The Burnham Institute, 10901 N Torrey Pines, La Jolla, CA 92037, USA
    Neoplasia 3:70-9. 2001
    ....
  36. ncbi p53-inducible human homologue of Drosophila seven in absentia (Siah) inhibits cell growth: suppression by BAG-1
    S Matsuzawa
    Burnham Institute, La Jolla, CA 92037, USA
    EMBO J 17:2736-47. 1998
    ..We suggest that Siah-1A may be an important mediator of p53-dependent cell-cycle arrest and demonstrate that Siah-1A is directly inhibited by BAG-1...
  37. ncbi TNFR-associated factor family protein expression in normal tissues and lymphoid malignancies
    J M Zapata
    The Burnham Institute, Program on Apoptosis and Cell Death Regulation, La Jolla, California 92037, USA
    J Immunol 165:5084-96. 2000
    ..01), as determined by immunoblotting. These findings contribute to an improved understanding of the cell-specific roles of TRAFs in normal tissues and provide evidence of altered TRAF1 expression in lymphoid malignancies...
  38. ncbi Bcl-2 family proteins and mitochondria
    J C Reed
    The Burnham Institute, Program on Apoptosis and Cell Death Research, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
    Biochim Biophys Acta 1366:127-37. 1998
    ....
  39. ncbi An evolutionarily conserved family of Hsp70/Hsc70 molecular chaperone regulators
    S Takayama
    The Burnham Institute, La Jolla, California 92037, USA
    J Biol Chem 274:781-6. 1999
    ..The findings suggest opportunities for specification and diversification of Hsp70/Hsc70 chaperone functions through interactions with various BAG-family proteins...
  40. ncbi A novel enhancer of the Apaf1 apoptosome involved in cytochrome c-dependent caspase activation and apoptosis
    Z L Chu
    Burnham Institute, La Jolla, California 92037, USA
    J Biol Chem 276:9239-45. 2001
    ..NAC expression in vivo is associated with terminal differentiation of short lived cells in epithelia and some other tissues. The ability of NAC to enhance Apaf1-apoptosome function reveals a novel paradigm for apoptosis regulation...
  41. ncbi TRAF2-binding BIR1 domain of c-IAP2/MALT1 fusion protein is essential for activation of NF-kappaB
    J B Garrison
    Program on Apoptosis and Cell Death Research, Cancer Center, Burnham Institute for Medical Research, La Jolla, CA 92037, USA
    Oncogene 28:1584-93. 2009
    ....
  42. ncbi Bag1 is a regulator and marker of neuronal differentiation
    P Kermer
    The Burnham Institute, Program on Apoptosis and Cell Death Research, 10901 N Torrey Pines Road, La Jolla, California, CA 92037, USA
    Cell Death Differ 9:405-13. 2002
    ..Taken together, these findings raise the possibility that the Bag1 protein is expressed early in neurogenesis in vivo and is capable of modulating neuronal cell survival and differentiation at least in part from a nuclear location...
  43. ncbi Thymidine-phosphorothioate oligonucleotides induce activation and apoptosis of CLL cells independently of CpG motifs or BCL-2 gene interference
    J E Castro
    John and Rebecca Moores Cancer Center, University of California San Diego, La Jolla, CA, USA
    Leukemia 20:680-8. 2006
    ..We conclude that thymidine-containing PS-ODN can activate CLL cells and induce apoptosis via a mechanism that is independent of BCL-2 gene interference or CpG motifs...
  44. ncbi Drosophila pro-apoptotic Bcl-2/Bax homologue reveals evolutionary conservation of cell death mechanisms
    H Zhang
    Program on Apoptosis and Cell Death Regulation, Burnham Institute, La Jolla, California 92037, USA
    J Biol Chem 275:27303-6. 2000
    ..Moreover, DBok targets mitochondria and triggers cytochrome c release through a caspase-independent mechanism. These characteristics of DBok reveal evolutionary conservation of cell death mechanisms in flies and humans...
  45. ncbi Characterization of interactions between the anti-apoptotic protein BAG-1 and Hsc70 molecular chaperones
    J K Stuart
    Burnham Institute, Cancer Research Center, La Jolla, California 92037, USA
    J Biol Chem 273:22506-14. 1998
    ..Molecular modeling permitted a comparison of structural features between the functionally homologous BAG-1 and GrpE proteins. These data were used to propose a mechanism for BAG-1 in the regulation of Hsp70/Hsc70 chaperone activity...
  46. ncbi Bcl-G, a novel pro-apoptotic member of the Bcl-2 family
    B Guo
    Burnham Institute, La Jolla, California 92037, USA
    J Biol Chem 276:2780-5. 2001
    ..A mutant of Bcl-G(L) in which the BH2 domain was deleted displayed increased apoptotic activity and coimmunoprecipitated with Bcl-X(L), suggesting that the BH2 domain autorepresses Bcl-G(L)...
  47. ncbi Bax directly induces release of cytochrome c from isolated mitochondria
    J M Jurgensmeier
    Program on Apoptosis and Cell Death Research, The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 95:4997-5002. 1998
    ....
  48. ncbi Cytochrome c release and apoptosis induced by mitochondrial targeting of nuclear orphan receptor TR3
    H Li
    The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
    Science 289:1159-64. 2000
    ..Our results reveal a mechanism by which a nuclear transcription factor translocates to mitochondria to initiate apoptosis...
  49. ncbi BAG1L enhances trans-activation function of the vitamin D receptor
    M Guzey
    Burnham Institute, La Jolla, California 92037 and RIGEB, MAM TUBITAK, P K 21 Gebze 41 470, Kocaeli, Turkey
    J Biol Chem 275:40749-56. 2000
    ..Thus, BAG1L is a direct regulator of VDR, which enhances its trans-activation function and improves tumor cell responses to growth-suppressive VDR ligands...
  50. ncbi A single BIR domain of XIAP sufficient for inhibiting caspases
    R Takahashi
    Burnham Institute, Program on Apoptosis and Cell Death Regulation, La Jolla, California 92037, USA
    J Biol Chem 273:7787-90. 1998
    ..These findings identify BIR2 as the minimal caspase-inhibitory domain of XIAP and indicate that a single BIR domain can be sufficient for binding and inhibiting caspases...
  51. ncbi TRAF family proteins interact with the common neurotrophin receptor and modulate apoptosis induction
    X Ye
    The Burnham Institute, La Jolla, California 92037, USA
    J Biol Chem 274:30202-8. 1999
    ..These results demonstrate that TRAF family proteins interact with p75(NTR) and differentially modulate its NF-kappaB activation and cell death induction...
  52. ncbi The Drosophila tumor necrosis factor receptor-associated factor-1 (DTRAF1) interacts with Pelle and regulates NFkappaB activity
    J M Zapata
    The Burnham Institute, La Jolla, California 92037, USA
    J Biol Chem 275:12102-7. 2000
    ..Interactions of DTRAF1 with human TRAF-, TNF receptor-, and IAP-family proteins imply strong evolutionary conservation of TRAF protein structure and function throughout Metazoan evolution...
  53. ncbi A short Nur77-derived peptide converts Bcl-2 from a protector to a killer
    Siva Kumar Kolluri
    Cancer Center, Burnham Institute for Medical Research, La Jolla, CA 92037, USA
    Cancer Cell 14:285-98. 2008
    ..NuBCP-9s act as molecular switches to dislodge the Bcl-2 BH4 domain, exposing its BH3 domain, which in turn blocks the activity of antiapoptotic Bcl-X(L)...
  54. ncbi Discovery, characterization, and structure-activity relationships studies of proapoptotic polyphenols targeting B-cell lymphocyte/leukemia-2 proteins
    Shinichi Kitada
    The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, California 92037, USA
    J Med Chem 46:4259-64. 2003
    ....
  55. ncbi An inducible pathway for degradation of FLIP protein sensitizes tumor cells to TRAIL-induced apoptosis
    Youngsoo Kim
    Burnham Institute, La Jolla, California 92037, USA
    J Biol Chem 277:22320-9. 2002
    ....
  56. ncbi Expression and potential role of Fas-associated phosphatase-1 in ovarian cancer
    I Meinhold-Heerlein
    Program on Apoptosis and Cell Death Research, The Burnham Institute, 10901 North Torrey Pines Rd, La Jolla, CA 92037, USA
    Am J Pathol 158:1335-44. 2001
    ..We conclude that FAP-1 correlates significantly with Fas resistance in ovarian cancer cell lines and is commonly expressed in ovarian cancers...
  57. ncbi Molecular basis for CD40 signaling mediated by TRAF3
    C Z Ni
    Cancer Center, The Burnham Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 97:10395-9. 2000
    ..TRAF2 suggests that CD40 may assume different conformations when bound to different TRAF family members. This molecular adaptation may influence binding affinity and specific cellular triggers...
  58. ncbi TRAF-4 expression in epithelial progenitor cells. Analysis in normal adult, fetal, and tumor tissues
    M Krajewska
    Burnham Institute, La Jolla, CA 92037, USA
    Am J Pathol 152:1549-61. 1998
    ..Although also expressed in some types of mesenchymal cells, these findings suggest that TRAF-4 is a marker of normal epithelial stem cells, the expression of which often ceases on differentiation and malignant transformation...
  59. ncbi Interaction of BAG-1 with retinoic acid receptor and its inhibition of retinoic acid-induced apoptosis in cancer cells
    R Liu
    The Burnham Institute, Cancer Research Center, La Jolla, California 92037, USA
    J Biol Chem 273:16985-92. 1998
    ..These results demonstrate that BAG-1 can regulate retinoid activities through its interaction with RAR and suggest that elevated levels of BAG-1 protein could potentially contribute to retinoid resistance in cancer cells...
  60. ncbi Developmental expression patterns of Bcl-2, Bcl-x, Bax, and Bak in teeth
    S Krajewski
    The Burnham Institute formerly La Jolla Cancer Research Foundation, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
    Cell Death Differ 5:408-15. 1998
    ....
  61. ncbi BAG-1L protein enhances androgen receptor function
    B A Froesch
    Burnham Institute, La Jolla, California 92037, USA
    J Biol Chem 273:11660-6. 1998
    ..These findings implicate BAG-1L in the regulation of AR function and may have relevance to mechanisms of prostate cancer resistance to hormone-ablative and anti-androgen therapy...
  62. ncbi Mechanisms of apoptosis
    J C Reed
    Burnham Institute, La Jolla, California 92037, USA
    Am J Pathol 157:1415-30. 2000
    ..Knowledge of the molecular mechanisms of apoptosis is providing insights into the causes of multiple pathologies where aberrant cell death regulation occurs and is beginning to provide new approaches to the treatment of human diseases...
  63. ncbi Bcl-2 family proteins
    J C Reed
    The Burnham Institute, La Jolla, California 92037, USA
    Oncogene 17:3225-36. 1998
    ....
  64. ncbi X-linked IAP is a direct inhibitor of cell-death proteases
    Q L Deveraux
    The Burnham Institute, Program on Apoptosis and Cell Death Research, La Jolla, California 92037, USA
    Nature 388:300-4. 1997
    ....
  65. ncbi Synthetic triterpenoids activate a pathway for apoptosis in AML cells involving downregulation of FLIP and sensitization to TRAIL
    W-S Suh
    The Burnham Institute, La Jolla, CA 92037, USA
    Leukemia 17:2122-9. 2003
    ..The findings suggest that synthetic triterpenoids warrant further investigation in the treatment of AML, alone or in combination with TRAIL or other immune-based therapies...
  66. ncbi PAAD - a new protein domain associated with apoptosis, cancer and autoimmune diseases
    K Pawłowski
    Program in Bioinformatics and Biological Complexity, The Burnham Institute, 10901 N Torrey Pines Road, La Jolla, CA 92037, USA
    Trends Biochem Sci 26:85-7. 2001
    ..Its location within these proteins and predicted fold suggests that it functions as a protein-protein interaction domain, possibly uniting different signaling pathways...
  67. ncbi Conversion of Bcl-2 from protector to killer by interaction with nuclear orphan receptor Nur77/TR3
    Bingzhen Lin
    The Burnham Institute, Cancer Center, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
    Cell 116:527-40. 2004
    ....
  68. ncbi Apoptosis-regulating proteins as targets for drug discovery
    J C Reed
    The Burnham Institute, La Jolla, CA 92037, USA
    Trends Mol Med 7:314-9. 2001
    ....
  69. ncbi BAG-1 modulates the chaperone activity of Hsp70/Hsc70
    S Takayama
    The Burnham Institute, Program on Apoptosis and Cell Death Research, La Jolla, CA 92037, USA
    EMBO J 16:4887-96. 1997
    ..The inhibitory effects of BAG-1 on Hsp/Hsc70 chaperone activity suggest that BAG-1 represents a novel type of chaperone regulatory proteins and thus suggest a link between cell signaling, cell death and the stress response...
  70. ncbi HBXIP functions as a cofactor of survivin in apoptosis suppression
    Hiroyuki Marusawa
    The Burnham Institute, La Jolla, CA 92037, USA
    EMBO J 22:2729-40. 2003
    ..Thus, HBXIP functions as a cofactor for survivin, and serves as a link between the cellular apoptosis machinery and a viral pathogen involved in hepatocellular carcinogenesis...
  71. ncbi Identification of small molecules that sensitize resistant tumor cells to tumor necrosis factor-family death receptors
    Aaron D Schimmer
    Burnham Institute for Medical Research, La Jolla, California 92037, USA
    Cancer Res 66:2367-75. 2006
    ....
  72. ncbi Triterpenoids display single agent anti-tumor activity in a transgenic mouse model of chronic lymphocytic leukemia and small B cell lymphoma
    Christina L Kress
    Burnham Institute for Medical Research, La Jolla, California, United States of America
    PLoS ONE 2:e559. 2007
    ..In this report, we have studied the effects of CDDO and its imidazolide derivative (CDDO-Im) on chronic lymphocytic leukemia (CLL), using patients' CLL cells and a mouse model of CLL and small B cell lymphoma (SBL)...
  73. ncbi Cellular, biochemical, and genetic analysis of mechanism of small molecule IAP inhibitors
    Zhiliang Wang
    The Burnham Institute, La Jolla, California 92037, USA
    J Biol Chem 279:48168-76. 2004
    ....
  74. ncbi Apoptosis-associated speck-like protein containing a caspase recruitment domain is a regulator of procaspase-1 activation
    Christian Stehlik
    Burnham Institute, La Jolla, CA 92037, USA
    J Immunol 171:6154-63. 2003
    ....
  75. ncbi Structurally distinct recognition motifs in lymphotoxin-beta receptor and CD40 for tumor necrosis factor receptor-associated factor (TRAF)-mediated signaling
    Chenglong Li
    Cancer Research Center, The Burnham Institute, La Jolla, California 92037, USA
    J Biol Chem 278:50523-9. 2003
    ..The results reveal structurally adaptive "hot spots" in the TRAF3-binding crevice that promote molecular interactions driving specific signaling after contact with LTbetaR, CD40, or the downstream regulator TANK...
  76. ncbi Functional role of death-associated protein 3 (DAP3) in anoikis
    Tadaaki Miyazaki
    The Burnham Institute, La Jolla, California 92037, USA
    J Biol Chem 279:44667-72. 2004
    ..Involvement of DAP3 in anoikis signaling demonstrates a novel role for this GTP-binding protein in apoptosis induction caused by cell detachment...
  77. ncbi Apogossypol derivatives as antagonists of antiapoptotic Bcl-2 family proteins
    Jun Wei
    Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 90237, USA
    Mol Cancer Ther 8:904-13. 2009
    ..Together with its improved plasma and microsomal stability relative to Apogossypol, BI79D10 represents a lead compound for the development of novel apoptosis-based therapies for cancer...
  78. ncbi Cytoprotective peptide humanin binds and inhibits proapoptotic Bcl-2/Bax family protein BimEL
    Frederic Luciano
    The Burnham Institute, La Jolla, California 92037, USA
    J Biol Chem 280:15825-35. 2005
    ..Taken together, our results indicate that the inhibition of BimEL may contribute to the antiapoptotic properties of the HN peptide...
  79. ncbi Cell death and endoplasmic reticulum stress: disease relevance and therapeutic opportunities
    InKi Kim
    Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Rev Drug Discov 7:1013-30. 2008
    ....
  80. ncbi CADD, a Chlamydia protein that interacts with death receptors
    Frank Stenner-Liewen
    Burnham Institute, La Jolla, California 92037, USA
    J Biol Chem 277:9633-6. 2002
    ..trachomatis and co-localizes with Fas in the proximity of the inclusion body. The results suggest a role for CADD modulating the apoptosis pathways of cells infected, revealing a new mechanism of host-pathogen interaction...
  81. ncbi Distinct BIR domains of cIAP1 mediate binding to and ubiquitination of tumor necrosis factor receptor-associated factor 2 and second mitochondrial activator of caspases
    Temesgen Samuel
    Burnham Institute for Medical Research, La Jolla, CA 92037, USA
    J Biol Chem 281:1080-90. 2006
    ....
  82. ncbi Gambogic acid is an antagonist of antiapoptotic Bcl-2 family proteins
    Dayong Zhai
    Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
    Mol Cancer Ther 7:1639-46. 2008
    ..Altogether, the findings suggest that suppression of antiapoptotic Bcl-2 family proteins may be among the cytotoxic mechanisms by which GA kills tumor cells...
  83. ncbi Bcl-2 antagonist apogossypol (NSC736630) displays single-agent activity in Bcl-2-transgenic mice and has superior efficacy with less toxicity compared with gossypol (NSC19048)
    Shinichi Kitada
    Burnham Institute for Medical Research, Cancer Research Center, La Jolla, CA 92037, USA
    Blood 111:3211-9. 2008
    ..Taken together, these studies indicate that apogossypol is superior to parent compound gossypol with respect to toxicology and efficacy, suggesting that further development of this compound for cancer therapy is warranted...
  84. ncbi Synthesis and biological evaluation of Apogossypolone derivatives as pan-active inhibitors of antiapoptotic B-cell lymphoma/leukemia-2 (Bcl-2) family proteins
    Jun Wei
    Sanford Burnham Medical Research Institute, 10901 North Torrey Pines Road, La Jolla, California 92037, USA
    J Med Chem 53:8000-11. 2010
    ..Together with its negligible toxicity, compound 6f represents a promising drug lead for the development of novel apoptosis-based therapies for cancer...
  85. ncbi ZIP kinase triggers apoptosis from nuclear PML oncogenic domains
    Taro Kawai
    The Burnham Institute, La Jolla, California 92037, USA
    Mol Cell Biol 23:6174-86. 2003
    ..These results suggest that ZIPK, in collaboration with Daxx and Par-4, mediates a novel nuclear pathway for apoptosis...
  86. ncbi Vaccinia virus virulence factor N1L is a novel promising target for antiviral therapeutic intervention
    Anton V Cheltsov
    Infectious and Inflammatory Disease Center, Burnham Institute for Medical Research, La Jolla, California 92037, USA
    J Med Chem 53:3899-906. 2010
    ..We have also identified the natural polyphenol resveratrol as a moderate N1L inhibitor. Finally, we show that our ligands efficiently inhibit growth of vaccinia virus...
  87. ncbi Elevated expression of inhibitor of apoptosis proteins in prostate cancer
    Maryla Krajewska
    The Burnham Institute, La Jolla, California 92037, USA
    Clin Cancer Res 9:4914-25. 2003
    ..CONCLUSIONS: The findings demonstrate that tumor- associated elevations in the expression of several IAP family proteins occur as a frequent and early event in the etiology of prostate cancer...
  88. ncbi Vaccinia virus protein F1L is a caspase-9 inhibitor
    Dayong Zhai
    Sanford Burnham Medical Research Institute, La Jolla, California 92037, USA
    J Biol Chem 285:5569-80. 2010
    ....
  89. ncbi Small-molecule antagonists of apoptosis suppressor XIAP exhibit broad antitumor activity
    Aaron D Schimmer
    The Burnham Institute, La Jolla, CA 92037, USA
    Cancer Cell 5:25-35. 2004
    ..Active compounds also suppressed growth of established tumors in xenograft models in mice, while displaying little toxicity to normal tissues. These findings validate IAPs as targets for cancer drug discovery...
  90. ncbi Nur77 converts phenotype of Bcl-B, an antiapoptotic protein expressed in plasma cells and myeloma
    Frederic Luciano
    Burnham Institute for Medical Research, 10901 Torrey Pines Road, La Jolla, CA 92037, USA
    Blood 109:3849-55. 2007
    ..Because Bcl-B is abundantly expressed in plasma cells and some myelomas, these findings raise the possibility of exploiting the Nur77/Bcl-B mechanism for apoptosis for eradication of autoimmune plasma cells or myeloma...
  91. ncbi Peptides targeting caspase inhibitors
    Ingo Tamm
    The Burnham Institute, La Jolla, California 97037, USA
    J Biol Chem 278:14401-5. 2003
    ..Peptides interacting with XIAP could serve as prototypes for the design of low molecular weight modulators of apoptosis...
  92. ncbi Apoptosis induction by 1alpha,25-dihydroxyvitamin D3 in prostate cancer
    Meral Guzey
    Cancer Research Center, The Burnham Institute, La Jolla, California 92037, USA
    Mol Cancer Ther 1:667-77. 2002
    ..Thus, VD3 is capable of inhibiting expression of multiple antiapoptotic proteins in VDR-expressing prostate cancer cells, leading to activation of the mitochondrial pathway for apoptosis...
  93. ncbi The PAAD/PYRIN-family protein ASC is a dual regulator of a conserved step in nuclear factor kappaB activation pathways
    Christian Stehlik
    The Burnham Institute, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Exp Med 196:1605-15. 2002
    ..Our findings suggest that ASC modulates diverse NF-kappaB induction pathways by acting upon the IKK complex, implying a broad role for this and similar proteins containing PAAD domains in regulation of inflammatory responses...
  94. ncbi Latent sensitivity to Fas-mediated apoptosis after CD40 ligation may explain activity of CD154 gene therapy in chronic lymphocytic leukemia
    Peter Chu
    The Chronic Lymphocytic Leukemia Research Consortium, Biomedical Sciences Graduate Program, Division of Hematology Oncology, Stein Institute for Research on Aging, School of Medicine, University of California at San Diego, La Jolla, CA 92093 0663, USA
    Proc Natl Acad Sci U S A 99:3854-9. 2002
    ....
  95. ncbi Identification and characterization of DEDD2, a death effector domain-containing protein
    Wilfried Roth
    The Burnham Institute, 10901 N Torrey Pines Road, La Jolla, CA 92037, USA
    J Biol Chem 277:7501-8. 2002
    ..These findings suggest that DEDD2 is involved in the regulation of nuclear events mediated by the extrinsic apoptosis pathway...
  96. ncbi Functional blocks in caspase activation pathways are common in leukemia and predict patient response to induction chemotherapy
    Aaron D Schimmer
    The Burnham Institute, La Jolla, California 92037, USA
    Cancer Res 63:1242-8. 2003
    ..This study supports the importance of apoptosis pathways in determining response to chemotherapy and suggests that functional defects in caspase activation are prognostic in patients with leukemia...
  97. ncbi Bag1 proteins regulate growth and survival of ZR-75-1 human breast cancer cells
    Masafumi Kudoh
    The Burnham Institute, La Jolla, California 92037, USA
    Cancer Res 62:1904-9. 2002
    ..Altogether, these findings demonstrate that Bag1 and Bag1L provoke similar changes in breast cancer cell growth and survival and suggest that interference with Bag1 or Bag1L function might be a useful strategy for opposing breast cancer...
  98. ncbi Fragment-based design of small molecule X-linked inhibitor of apoptosis protein inhibitors
    Jui Wen Huang
    Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, California 92037, USA
    J Med Chem 51:7111-8. 2008
    ..Hence, as an application, we report on the derivation of novel, selective, druglike, cell permeable SMAC mimics with cellular activity...
  99. ncbi Conformation of membrane-associated proapoptotic tBid
    Xiao Min Gong
    Burnham Institute, La Jolla, California 92037, USA
    J Biol Chem 279:28954-60. 2004
    ....
  100. ncbi Synthetic triterpenoids cooperate with tumor necrosis factor-related apoptosis-inducing ligand to induce apoptosis of breast cancer cells
    Marc L Hyer
    The Burnham Institute, La Jolla, California 92037, USA
    Cancer Res 65:4799-808. 2005
    ..These data suggest that CDDO and CDDO-Im may be useful for selectively reversing the TRAIL-resistant phenotype in cancer but not normal cells...
  101. ncbi Key molecular contacts promote recognition of the BAFF receptor by TNF receptor-associated factor 3: implications for intracellular signaling regulation
    Chao Zhou Ni
    Cancer Research Center, The Burnham Institute, La Jolla, CA 92037, USA
    J Immunol 173:7394-400. 2004
    ..The structure of the complex provides a molecular explanation for binding affinities and selective protein interactions in TNFR-TRAF interactions...

Research Grants55

  1. MITOCHONDRIA AND APOPTOSIS
    John Reed; Fiscal Year: 2007
    ....
  2. Yeast-based HTS Assay Technologies for Proteases
    John Reed; Fiscal Year: 2009
    ..For proof of concept, we focus on proteases important for inflammatory and infectious diseases. ..
  3. Chemical Antagonists of IAP-Family Anti-Apoptotic Proteins
    John Reed; Fiscal Year: 2007
    ..The resulting compounds will be useful as research tools for understanding the biology of IAPs and for ascertaining their roles in diseases such as cancer, where IAP over-expression is commonly observed. ..
  4. SIGNAL TRANSDUCTION AND CELL DEATH REGULATION
    John Reed; Fiscal Year: 2007
    ....
  5. Apoptosis-Based Cancer Drug Discovery
    John Reed; Fiscal Year: 2007
    ..This knowledge-base can now be exploited for devising strategies for small molecule drug discovery, towards the goal of revolutionary new treatments for cancer and leukemia. ..
  6. Mechanisms of the HBX-interacting protein (HBXIP)
    John Reed; Fiscal Year: 2007
    ..Altogether, these investigations will define the role of HBXIP in regulating cell division and apoptosis, as well as providing new insights into the mechanisms of HBx in the pathogenesis of HCC. ..
  7. PAAD-Family Proteins and Host Defense Mechanisms
    John Reed; Fiscal Year: 2007
    ..abstract_text> ..
  8. Chemical Inhibitors of ER Stress
    John Reed; Fiscal Year: 2007
    ....
  9. NLR Family Proteins: Mechanisms and Regulation
    John Reed; Fiscal Year: 2009
    ..Thus, studies of NLRs have relevance to a wide diversity of human diseases. ..
  10. BI-1, A Regulator of ER-Stress Pathways Linked to Apoptosis
    John Reed; Fiscal Year: 2009
    ..Altogether, these studies will improve understanding of how damage to the ER is linked to apoptosis, and will potentially reveal strategies for neuronal preservation in stroke and neurodegenerative diseases. ..
  11. Mitochondria and Apoptosis (revised GM60554)
    John Reed; Fiscal Year: 2009
    ..abstract_text> ..
  12. BAG-1--A NOVEL BCL-2 BINDING PROTEIN
    John Reed; Fiscal Year: 1999
    ....
  13. BAX INHIBITORY PROTEINS, BI1 AND BI2
    John Reed; Fiscal Year: 2002
    ....
  14. CED-4 FAMILY PROTEINS
    John Reed; Fiscal Year: 2003
    ..The information provided by these investigations may provide insights into human diseases where dysregulation of programmed cell death is known to occur and may lead to new strategies for therapeutic intervention. ..
  15. MITOCHONDRIA AND APOPTOSIS
    John Reed; Fiscal Year: 2003
    ..g. myocardial infarction, stroke; AIDS; neurodegeneration), the insights gains from these investigations could find broad applicability to the improved treatment of several human diseases. ..