Alexander Ploss

Summary

Affiliation: The Rockefeller University
Country: USA

Publications

  1. pmc Towards a small animal model for hepatitis C
    Alexander Ploss
    Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York 10065, USA
    EMBO Rep 10:1220-7. 2009
  2. pmc A genetically humanized mouse model for hepatitis C virus infection
    Marcus Dorner
    Center for the Study of Hepatitis C, The Rockefeller University, New York, New York 10065, USA
    Nature 474:208-11. 2011
  3. pmc Hepatitis C virus host cell entry
    Alexander Ploss
    Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Diseases, The Rockefeller University, New York, NY 10065, USA
    Curr Opin Virol 2:14-9. 2012
  4. doi request reprint New advances in the molecular biology of hepatitis C virus infection: towards the identification of new treatment targets
    Alexander Ploss
    The Rockefeller University, Center for the Study of Hepatitis C, Laboratory for Virology and Infectious Diseases, 1230 York Avenue, Box 64, New York 10065, USA
    Gut 61:i25-35. 2012
  5. pmc Expression of paramyxovirus V proteins promotes replication and spread of hepatitis C virus in cultures of primary human fetal liver cells
    Linda Andrus
    Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Diseases, The Rockefeller University, New York, NY 10021, USA
    Hepatology 54:1901-12. 2011
  6. pmc Completion of the entire hepatitis C virus life cycle in genetically humanized mice
    Marcus Dorner
    Center for the Study of Hepatitis C, The Rockefeller University, New York, New York 10065, USA
    Nature 501:237-41. 2013
  7. pmc Recapitulation of the hepatitis C virus life-cycle in engineered murine cell lines
    Alexander Vogt
    Center for the Study of Hepatitis C, The Rockefeller University, New York, NY 10065, USA
    Virology 444:1-11. 2013
  8. pmc Utility of humanized BLT mice for analysis of dengue virus infection and antiviral drug testing
    Natalia Frias-Staheli
    Center for the Study of Hepatitis C, Laboratory for Virology and Infectious Disease, The Rockefeller University, New York, New York, USA
    J Virol 88:2205-18. 2014
  9. pmc Persistent hepatitis C virus infection in microscale primary human hepatocyte cultures
    Alexander Ploss
    Center for the Study of Hepatitis C and Laboratory of Virology and Infectious Diseases, The Rockefeller University, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 107:3141-5. 2010
  10. pmc Expression of heterologous proteins flanked by NS3-4A cleavage sites within the hepatitis C virus polyprotein
    Joshua A Horwitz
    Center for the Study of Hepatitis C, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Virology 439:23-33. 2013

Collaborators

Detail Information

Publications30

  1. pmc Towards a small animal model for hepatitis C
    Alexander Ploss
    Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York 10065, USA
    EMBO Rep 10:1220-7. 2009
    ..We highlight three independent, but possibly complementary, approaches towards overcoming current species barriers and generating a small-animal model for HCV pathogenesis...
  2. pmc A genetically humanized mouse model for hepatitis C virus infection
    Marcus Dorner
    Center for the Study of Hepatitis C, The Rockefeller University, New York, New York 10065, USA
    Nature 474:208-11. 2011
    ....
  3. pmc Hepatitis C virus host cell entry
    Alexander Ploss
    Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Diseases, The Rockefeller University, New York, NY 10065, USA
    Curr Opin Virol 2:14-9. 2012
    ..A detailed understanding of the viral uptake pathway is imperative to define new drug targets allowing for more effective intervention against this devastating disease...
  4. doi request reprint New advances in the molecular biology of hepatitis C virus infection: towards the identification of new treatment targets
    Alexander Ploss
    The Rockefeller University, Center for the Study of Hepatitis C, Laboratory for Virology and Infectious Diseases, 1230 York Avenue, Box 64, New York 10065, USA
    Gut 61:i25-35. 2012
    ..Such studies have revealed a growing list of targets for therapeutic intervention, some of which will be discussed in this review...
  5. pmc Expression of paramyxovirus V proteins promotes replication and spread of hepatitis C virus in cultures of primary human fetal liver cells
    Linda Andrus
    Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Diseases, The Rockefeller University, New York, NY 10021, USA
    Hepatology 54:1901-12. 2011
    ..Strategies aimed at dampening this response may be key to further development of robust HCV culture systems, enabling studies of virus pathogenicity and the mechanisms by which HCV spreads in its natural host cell population...
  6. pmc Completion of the entire hepatitis C virus life cycle in genetically humanized mice
    Marcus Dorner
    Center for the Study of Hepatitis C, The Rockefeller University, New York, New York 10065, USA
    Nature 501:237-41. 2013
    ....
  7. pmc Recapitulation of the hepatitis C virus life-cycle in engineered murine cell lines
    Alexander Vogt
    Center for the Study of Hepatitis C, The Rockefeller University, New York, NY 10065, USA
    Virology 444:1-11. 2013
    ..Our data demonstrate that the barriers of HCV interspecies transmission can be overcome by engineering a suitable cellular environment and provide a blue-print towards constructing a small animal model for HCV infection. ..
  8. pmc Utility of humanized BLT mice for analysis of dengue virus infection and antiviral drug testing
    Natalia Frias-Staheli
    Center for the Study of Hepatitis C, Laboratory for Virology and Infectious Disease, The Rockefeller University, New York, New York, USA
    J Virol 88:2205-18. 2014
    ..We further show that this mouse model can be used to test preclinically the efficacy of antiviral drugs. ..
  9. pmc Persistent hepatitis C virus infection in microscale primary human hepatocyte cultures
    Alexander Ploss
    Center for the Study of Hepatitis C and Laboratory of Virology and Infectious Diseases, The Rockefeller University, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 107:3141-5. 2010
    ..When coupled with highly sensitive fluorescence- and luminescence-based reporter systems, MPCCs have potential as a high-throughput platform for simultaneous assessment of in vitro efficacy and toxicity profiles of anti-HCV therapeutics...
  10. pmc Expression of heterologous proteins flanked by NS3-4A cleavage sites within the hepatitis C virus polyprotein
    Joshua A Horwitz
    Center for the Study of Hepatitis C, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Virology 439:23-33. 2013
    ..Expression of heterologous proteins within the HCV genome offers new opportunities to analyze HCV infection in experimental systems without perturbing functions of individual viral proteins...
  11. pmc New horizons for studying human hepatotropic infections
    Ype P De Jong
    Center for the Study of Hepatitis C, The Rockefeller University, New York, New York 10065, USA
    J Clin Invest 120:650-3. 2010
    ....
  12. pmc Real-time imaging of hepatitis C virus infection using a fluorescent cell-based reporter system
    Christopher T Jones
    Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York, USA
    Nat Biotechnol 28:167-71. 2010
    ..Integration of this reporter with modern image-based analysis methods could open new doors for HCV research...
  13. pmc Antibody and antiretroviral preexposure prophylaxis prevent cervicovaginal HIV-1 infection in a transgenic mouse model
    Henning Gruell
    Laboratory of Molecular Immunology, The Rockefeller University, New York, New York, USA
    J Virol 87:8535-44. 2013
    ..It further supports the idea that broadly neutralizing antibodies should be evaluated for use as preexposure prophylaxis in clinical trials. ..
  14. pmc Characterization of human antiviral adaptive immune responses during hepatotropic virus infection in HLA-transgenic human immune system mice
    Eva Billerbeck
    Laboratory of Virology and Infectious Disease, Center for the Study of Hepatitis C, The Rockefeller University, New York, NY 10065, USA
    J Immunol 191:1753-64. 2013
    ..The adenovirus reporter system used in our study may serve as simple in vivo method to evaluate future strategies for improving human intrahepatic immune responses in humanized mice. ..
  15. pmc Development of human CD4+FoxP3+ regulatory T cells in human stem cell factor-, granulocyte-macrophage colony-stimulating factor-, and interleukin-3-expressing NOD-SCID IL2Rγ(null) humanized mice
    Eva Billerbeck
    Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY, USA
    Blood 117:3076-86. 2011
    ....
  16. pmc HIV-1 suppression and durable control by combining single broadly neutralizing antibodies and antiretroviral drugs in humanized mice
    Joshua A Horwitz
    Laboratory of Molecular Immunology, Laboratory of Virology and Infectious Diseases, and Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10065
    Proc Natl Acad Sci U S A 110:16538-43. 2013
    ..We conclude that immunotherapy reduces plasma viral load and cell-associated HIV-1 DNA and that decreasing the initial viral load enables single bNAbs to control viremia in hu-mice. ..
  17. doi request reprint Advances and challenges in studying hepatitis C virus in its native environment
    Timothy Sheahan
    Center for the Study of Hepatitis C, The Rockefeller University, 1230 York Avenue, Box 64, New York, NY 10065, USA
    Expert Rev Gastroenterol Hepatol 4:541-50. 2010
    ..Here, we discuss recent advances in culture and detection systems that facilitate the study of HCV in primary cells. Use of these new models may help bridge the gap between in vitro studies and clinical research...
  18. pmc Hepatitis C virus induces interferon-λ and interferon-stimulated genes in primary liver cultures
    Svetlana Marukian
    Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Disease, Rockefeller University, New York, NY 10065, USA
    Hepatology 54:1913-23. 2011
    ..Induction of ISGs and λ IFNs may limit the growth and spread of HCV in primary cell cultures and in the infected liver. HCV infection of HFLC may provide a useful model for the study of gene induction by HCV in vivo...
  19. pmc Human occludin is a hepatitis C virus entry factor required for infection of mouse cells
    Alexander Ploss
    Center for the Study of Hepatitis C, The Rockefeller University, New York, New York 10065, USA
    Nature 457:882-6. 2009
    ..The identification of OCLN as a new HCV entry factor further highlights the importance of the tight junction complex in the viral entry process, and provides an important advance towards efforts to develop small animal models for HCV...
  20. ncbi request reprint Interferon lambda alleles predict innate antiviral immune responses and hepatitis C virus permissiveness
    Timothy Sheahan
    Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Diseases, The Rockefeller University, New York, NY 10065, USA
    Cell Host Microbe 15:190-202. 2014
    ..These data suggest that early virus-host interactions, particularly host genetics and induction of innate immunity, critically determine the outcome of HCV infection. ..
  21. pmc Study of hepatitis C virus entry in genetically humanized mice
    Marcus Dorner
    Center for the Study of Hepatitis C, Laboratory for Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA
    Methods 59:249-57. 2013
    ..Here, we provide a detailed protocol for the generation of these animals and highlight some of its applications for studying HCV biology and preclinical testing of drug and vaccine candidates...
  22. pmc HIV therapy by a combination of broadly neutralizing antibodies in humanized mice
    Florian Klein
    Laboratory of Molecular Immunology, The Rockefeller University, New York, New York 10065, USA
    Nature 492:118-22. 2012
    ..Thus, combinations of potent monoclonal antibodies can effectively control HIV-1 replication in humanized mice, and should be re-examined as a therapeutic modality in HIV-1-infected individuals...
  23. pmc Cell culture-grown hepatitis C virus is infectious in vivo and can be recultured in vitro
    Brett D Lindenbach
    Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Disease, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 103:3805-9. 2006
    ..These results greatly extend the utility of the HCVcc genetic system to allow the complete in vitro and in vivo dissection of the HCV life cycle...
  24. doi request reprint Animal models for hepatitis C
    Eva Billerbeck
    Center for the Study of Hepatitis C, The Rockefeller University, NY, USA
    Curr Top Microbiol Immunol 369:49-86. 2013
    ..Exciting new models and improvements in established models hold promise to further elucidate our understanding of chronic HCV infection...
  25. doi request reprint Hepatitis C virus vaccines in the era of new direct-acting antivirals
    Chao Shi
    Laboratory of Virology and Infectious Disease, Center for the Study of Hepatitis C The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Expert Rev Gastroenterol Hepatol 7:171-85. 2013
    ..Consequently, development of novel therapeutic and prophylactic strategies is imperative to contain HCV infection globally...
  26. pmc Barriers of hepatitis C virus interspecies transmission
    Lisa Sandmann
    Laboratory of Virology and Infectious Disease, Center for the Study of Hepatitis C, The Rockefeller University, New York, NY 10065, USA
    Virology 435:70-80. 2013
    ..Some progress has been made elucidating the barriers for the HCV lifecycle in non-permissive species which will help in the future to construct animal models for HCV infection, immunity and pathogenesis...
  27. doi request reprint Deconstructing hepatitis C virus infection in humanized mice
    Marcus Dorner
    Center for the Study of Hepatitis C, Laboratory for Virology and Infectious Disease, The Rockefeller University, New York, New York, USA
    Ann N Y Acad Sci 1245:59-62. 2011
    ..Hepatitis C virus is a major medical problem. Novel small-animal models are likely to accelerate the development of more effective therapeutics and a vaccine...
  28. pmc Dengue reporter viruses reveal viral dynamics in interferon receptor-deficient mice and sensitivity to interferon effectors in vitro
    John W Schoggins
    Laboratory of Virology and Infectious Disease, Center for the Study of Hepatitis C, The Rockefeller University, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 109:14610-5. 2012
    ..Several antiviral effectors were identified, and they targeted DENV at two distinct life cycle steps. These viruses provide a powerful platform for applications ranging from validation of vaccine candidates to antiviral discovery...
  29. pmc Priming of protective T cell responses against virus-induced tumors in mice with human immune system components
    Till Strowig
    Laboratory of Viral Immunobiology, Christopher H Browne Center for Immunology and Immune Diseases, The Rockefeller University, New York, NY 10065, USA
    J Exp Med 206:1423-34. 2009
    ..Both loss of CD4(+) and CD8(+) T cells abolished immune control. Therefore, this mouse model recapitulates features of symptomatic primary EBV infection and generates T cell-mediated immune control that resists oncogenic transformation...
  30. pmc A mouse model for HIV-1 entry
    John Pietzsch
    Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 109:15859-64. 2012
    ..An antibody's ability to block viral entry in this in vivo model is a function of its bioavailability, direct neutralizing activity, and effector functions...