Ardem Patapoutian

Summary

Affiliation: The Scripps Research Institute
Country: USA

Publications

  1. ncbi request reprint Opposite thermosensor in fruitfly and mouse
    Veena Viswanath
    Genomics Institute of the Novartis Research Foundation, San Diego, California 92121, USA
    Nature 423:822-3. 2003
  2. ncbi request reprint ThermoTRP channels and beyond: mechanisms of temperature sensation
    Ardem Patapoutian
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Nat Rev Neurosci 4:529-39. 2003
  3. pmc Single residues in the outer pore of TRPV1 and TRPV3 have temperature-dependent conformations
    Sung Eun Kim
    Department of Molecular and Cellular Neuroscience, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, California, United States of America
    PLoS ONE 8:e59593. 2013
  4. ncbi request reprint Roles of Wnt proteins in neural development and maintenance
    A Patapoutian
    Department of Cell Biology, MB6, The Scripps Research Institute, La Jolla, CA 92037
    Curr Opin Neurobiol 10:392-9. 2000
  5. ncbi request reprint TRP channels and thermosensation
    Ardem Patapoutian
    Department of Cell Biology, ICND202, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Chem Senses 30:i193-4. 2005
  6. ncbi request reprint Trk receptors: mediators of neurotrophin action
    A Patapoutian
    Department of Cell Biology, The Scripps Research Institute and Genomics Institute, Novartis Research Foundation, La Jolla, CA 92037, USA
    Curr Opin Neurobiol 11:272-80. 2001
  7. pmc TRPV1 is activated by both acidic and basic pH
    Ajay Dhaka
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Neurosci 29:153-8. 2009
  8. ncbi request reprint Impaired thermosensation in mice lacking TRPV3, a heat and camphor sensor in the skin
    Aziz Moqrich
    Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92037, USA
    Science 307:1468-72. 2005
  9. pmc Piezo1 and Piezo2 are essential components of distinct mechanically activated cation channels
    Bertrand Coste
    Department of Cell Biology, The Scripps Research Institute TSRI, La Jolla, CA 92037, USA
    Science 330:55-60. 2010
  10. ncbi request reprint More than cool: promiscuous relationships of menthol and other sensory compounds
    Lindsey J Macpherson
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Cell Neurosci 32:335-43. 2006

Research Grants

  1. Somatic Sensory Neurons: Mechanisms of Heterogeneity
    Ardem Patapoutian; Fiscal Year: 2002
  2. Nociceptive Ion Channels: mechanisms of activation
    Ardem Patapoutian; Fiscal Year: 2009
  3. Molecular characterization of Sensory Function
    Ardem Patapoutian; Fiscal Year: 2009
  4. Temperature-activated TRP channels and thermosensation
    Ardem Patapoutian; Fiscal Year: 2007
  5. Molecular characterization of Sensory Function
    Ardem Patapoutian; Fiscal Year: 2007
  6. Nociceptive Ion Channels: mechanisms of activation
    Ardem Patapoutian; Fiscal Year: 2006
  7. Molecular characterization of Sensory Function
    Ardem Patapoutian; Fiscal Year: 2006
  8. Temperature-activated TRP channels and thermosensation
    Ardem Patapoutian; Fiscal Year: 2006
  9. Somatic Sensory Neurons: Mechanisms of Heterogeneity
    Ardem Patapoutian; Fiscal Year: 2003
  10. Nociceptive Ion Channels: mechanisms of activation
    Ardem Patapoutian; Fiscal Year: 2010

Collaborators

Detail Information

Publications44

  1. ncbi request reprint Opposite thermosensor in fruitfly and mouse
    Veena Viswanath
    Genomics Institute of the Novartis Research Foundation, San Diego, California 92121, USA
    Nature 423:822-3. 2003
  2. ncbi request reprint ThermoTRP channels and beyond: mechanisms of temperature sensation
    Ardem Patapoutian
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Nat Rev Neurosci 4:529-39. 2003
  3. pmc Single residues in the outer pore of TRPV1 and TRPV3 have temperature-dependent conformations
    Sung Eun Kim
    Department of Molecular and Cellular Neuroscience, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, California, United States of America
    PLoS ONE 8:e59593. 2013
    ..Collectively, this suggests that these specific residues and the second predicted pore loop in general are crucial for the temperature-activation mechanism of these heat-activated thermoTRPs...
  4. ncbi request reprint Roles of Wnt proteins in neural development and maintenance
    A Patapoutian
    Department of Cell Biology, MB6, The Scripps Research Institute, La Jolla, CA 92037
    Curr Opin Neurobiol 10:392-9. 2000
    ..Wnt signaling has been demonstrated to regulate apoptosis and may participate in degenerative processes leading to cell death in the aging brain...
  5. ncbi request reprint TRP channels and thermosensation
    Ardem Patapoutian
    Department of Cell Biology, ICND202, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Chem Senses 30:i193-4. 2005
  6. ncbi request reprint Trk receptors: mediators of neurotrophin action
    A Patapoutian
    Department of Cell Biology, The Scripps Research Institute and Genomics Institute, Novartis Research Foundation, La Jolla, CA 92037, USA
    Curr Opin Neurobiol 11:272-80. 2001
    ..Three-dimensional structures of the Trk receptors, in one instance in association with a neurotrophin, have revealed the structural bases underlying specificity in neurotrophin signaling...
  7. pmc TRPV1 is activated by both acidic and basic pH
    Ajay Dhaka
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Neurosci 29:153-8. 2009
    ..Our studies show that TRPV1 detects both acidic and basic deviations from homeostatic pH...
  8. ncbi request reprint Impaired thermosensation in mice lacking TRPV3, a heat and camphor sensor in the skin
    Aziz Moqrich
    Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92037, USA
    Science 307:1468-72. 2005
    ..Camphor activated cultured primary keratinocytes but not sensory neurons, and this activity was abolished in TRPV3 null mice. Therefore, heat-activated receptors in keratinocytes are important for mammalian thermosensation...
  9. pmc Piezo1 and Piezo2 are essential components of distinct mechanically activated cation channels
    Bertrand Coste
    Department of Cell Biology, The Scripps Research Institute TSRI, La Jolla, CA 92037, USA
    Science 330:55-60. 2010
    ..Piezos are expressed in several tissues, and knockdown of Piezo2 in dorsal root ganglia neurons specifically reduced rapidly adapting MA currents. We propose that Piezos are components of MA cation channels...
  10. ncbi request reprint More than cool: promiscuous relationships of menthol and other sensory compounds
    Lindsey J Macpherson
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Cell Neurosci 32:335-43. 2006
    ..Therefore, we find that many "sensory compounds" presumed to be specific have a promiscuous relationship with thermoTRPs...
  11. pmc Identification of transmembrane domain 5 as a critical molecular determinant of menthol sensitivity in mammalian TRPA1 channels
    Bailong Xiao
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Neurosci 28:9640-51. 2008
    ..These data suggest separable structural requirements for modulation of TRPA1 by covalent and nonreactive molecules. Whether this region is involved in binding or gating of TRPA1 channels is discussed...
  12. pmc Piezo proteins are pore-forming subunits of mechanically activated channels
    Bertrand Coste
    Department of Cell Biology, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, California 92037, USA
    Nature 483:176-81. 2012
    ..These data demonstrate that Piezo proteins are an evolutionarily conserved ion channel family involved in mechanotransduction...
  13. pmc Inflammatory signals enhance piezo2-mediated mechanosensitive currents
    Adrienne E Dubin
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Cell Rep 2:511-7. 2012
    ..Finally, piezo2-dependent MA currents in a class of native sensory neurons are enhanced 8-fold by BK via PKA and PKC. Thus, piezo2 sensitization may contribute to PKA- and PKC-mediated mechanical hyperalgesia...
  14. pmc TRPV1 and TRPA1 mediate peripheral nitric oxide-induced nociception in mice
    Takashi Miyamoto
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California, USA
    PLoS ONE 4:e7596. 2009
    ..In behavioral assays, peripheral NO-induced nociception is compromised when TRPV1 and TRPA1 are both ablated. These results provide genetic evidence that the peripheral nociceptive action of NO is mediated by both TRPV1 and TRPA1...
  15. pmc Temperature-induced opening of TRPV1 ion channel is stabilized by the pore domain
    JORG GRANDL
    Department of Cell Biology, Scripps Research Institute, La Jolla, California, USA
    Nat Neurosci 13:708-14. 2010
    ..We propose that sequential occupancy of short and long open states on activation provides a mechanism for enhancing temperature sensitivity. Our results suggest that the outer pore is important for the heat sensitivity of thermoTRPs...
  16. doi request reprint Visualizing cold spots: TRPM8-expressing sensory neurons and their projections
    Ajay Dhaka
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Neurosci 28:566-75. 2008
    ..We show that TRPM8 expression functionally associates with cold sensitivity in cultured DRGs, and provide the first glimpses of the unique anatomical architecture of cold fibers in vivo...
  17. ncbi request reprint TRPM8 is required for cold sensation in mice
    Ajay Dhaka
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Neuron 54:371-8. 2007
    ..Therefore, depending on context, TRPM8 contributes to sensing unpleasant cold stimuli or mediating the effects of cold analgesia...
  18. pmc Pore region of TRPV3 ion channel is specifically required for heat activation
    JORG GRANDL
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Nat Neurosci 11:1007-13. 2008
    ..These findings demonstrate that the temperature sensitivity of TRPV3 is separable from all other known activation mechanisms and implicate a specific region in temperature sensing...
  19. ncbi request reprint An ion channel essential for sensing chemical damage
    Lindsey J Macpherson
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Neurosci 27:11412-5. 2007
    ..Therefore, although these reactive chemicals modify many proteins, the associated pain appears mainly dependent on a single ion channel...
  20. pmc Piezo2 is required for Merkel-cell mechanotransduction
    Seung Hyun Woo
    Howard Hughes Medical Institute, Molecular and Cellular Neuroscience, The Scripps Research Institute, La Jolla, California 92037, USA
    Nature 509:622-6. 2014
    ..The two-receptor system could provide this mechanoreceptor complex with a tuning mechanism to achieve highly sophisticated responses to a given mechanical stimulus. ..
  21. pmc Temperature-dependent STIM1 activation induces Ca²+ influx and modulates gene expression
    Bailong Xiao
    Dorris Neuroscience Center, Department of Cell Biology, The Scripps Research Institute, La Jolla, California, USA
    Nat Chem Biol 7:351-8. 2011
    ..Additionally, physiologically relevant temperature shifts modulate STIM1-dependent gene expression in Jurkat T cells. Therefore, temperature is an important regulator of STIM1 function...
  22. pmc Nociceptive signals induce trafficking of TRPA1 to the plasma membrane
    Manuela Schmidt
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Neuron 64:498-509. 2009
    ..Capacitance recordings suggest that MO can induce exocytosis. We propose that TRPA1 translocation to the membrane might represent one of the mechanisms controlling TRPA1 functionality upon acute activation or inflammatory signals...
  23. ncbi request reprint High-throughput random mutagenesis screen reveals TRPM8 residues specifically required for activation by menthol
    Michael Bandell
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Nat Neurosci 9:493-500. 2006
    ..In contrast, S2 mutations strongly shifted the concentration dependence of menthol activation, raising the possibility that S2 influences menthol binding...
  24. ncbi request reprint Noxious compounds activate TRPA1 ion channels through covalent modification of cysteines
    Lindsey J Macpherson
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Nature 445:541-5. 2007
    ..Collectively, our data indicate that covalent modification of reactive cysteines within TRPA1 can cause channel activation, rapidly signalling potential tissue damage through the pain pathway...
  25. ncbi request reprint The pungency of garlic: activation of TRPA1 and TRPV1 in response to allicin
    Lindsey J Macpherson
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Curr Biol 15:929-34. 2005
    ..We further show that allicin, an unstable component of fresh garlic, is the chemical responsible for TRPA1 and TRPV1 activation and is therefore likely to cause garlic's pungency...
  26. pmc The role of Drosophila Piezo in mechanical nociception
    Sung Eun Kim
    Department of Cell Biology, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, California 92037, USA
    Nature 483:209-12. 2012
    ..These data demonstrate the physiological relevance of the Piezo family in mechanotransduction in vivo, supporting a role of Piezo proteins in mechanosensory nociception...
  27. pmc TRPV3 regulates nitric oxide synthase-independent nitric oxide synthesis in the skin
    Takashi Miyamoto
    Department of Cell Biology, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, California 92037, USA
    Nat Commun 2:369. 2011
    ..Our study demonstrates that activation of an ion channel can induce NOS-independent NO production in keratinocytes...
  28. pmc Nociceptors: the sensors of the pain pathway
    Adrienne E Dubin
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California, USA
    J Clin Invest 120:3760-72. 2010
    ..Current work in this field is providing researchers with a more thorough understanding of nociceptor cell biology at molecular and systems levels and insight that will allow the targeted design of novel pain therapeutics...
  29. pmc From chills to chilis: mechanisms for thermosensation and chemesthesis via thermoTRPs
    Michael Bandell
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Curr Opin Neurobiol 17:490-7. 2007
    ..Recent studies have provided some striking new insights into the molecular mechanism of thermal and chemical activation of these biological thermometers...
  30. ncbi request reprint Trp ion channels and temperature sensation
    Ajay Dhaka
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Annu Rev Neurosci 29:135-61. 2006
    ..In this review, we examine the extent to which thermoTRPs are responsible for providing a molecular basis for thermal sensation...
  31. ncbi request reprint ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures
    Gina M Story
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Cell 112:819-29. 2003
    ..Consistent with the expression of ANKTM1, we identify noxious cold-sensitive sensory neurons that also respond to capsaicin but not to menthol...
  32. pmc Gain-of-function mutations in the mechanically activated ion channel PIEZO2 cause a subtype of Distal Arthrogryposis
    Bertrand Coste
    Molecular and Cellular Neuroscience Department, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 110:4667-72. 2013
    ..Our studies identify a type of channelopathy and link the dysfunction of mechanically activated ion channels to developmental malformations and joint contractures...
  33. ncbi request reprint A FAAH-regulated class of N-acyl taurines that activates TRP ion channels
    Alan Saghatelian
    Department of Cell Biology, The Skaggs Institute for Chemical Biology, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Biochemistry 45:9007-15. 2006
    ....
  34. pmc Expressing TrkC from the TrkA locus causes a subset of dorsal root ganglia neurons to switch fate
    Aziz Moqrich
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Nat Neurosci 7:812-8. 2004
    ..The surviving presumptive TrkA-expressing neurons adopted a proprioceptive phenotype, indicating that neurotrophin signaling can specify sensory neuron subtypes...
  35. pmc SWELL1, a plasma membrane protein, is an essential component of volume-regulated anion channel
    Zhaozhu Qiu
    Genomics Institute of the Novartis Research Foundation, San Diego, CA 92121, USA Department of Molecular and Cellular Neuroscience, Howard Hughes Medical Institute, The Scripps Research Institute, La Jolla, CA 92037, USA
    Cell 157:447-58. 2014
    ..These findings enable further molecular characterization of the VRAC channel complex and genetic studies for understanding the function of VRAC in normal physiology and disease...
  36. pmc Hypermorphic mutation of the voltage-gated sodium channel encoding gene Scn10a causes a dramatic stimulus-dependent neurobehavioral phenotype
    Amanda L Blasius
    Department of Genetics, and Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 108:19413-8. 2011
    ..Our data confirm the involvement of Na(v)1.8 in transducing pain initiated by cold and additionally implicate Na(v)1.8 in previously unknown functions in the central nervous system and heart...
  37. pmc Transient receptor potential channels: targeting pain at the source
    Ardem Patapoutian
    The Scripps Research Institute, 10550 North Torrey Pines Road, ICND210F, La Jolla, California 92037, USA
    Nat Rev Drug Discov 8:55-68. 2009
    ....
  38. ncbi request reprint Why does morphine make you itch?
    Takashi Miyamoto
    Department of Cell Biology, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, CA 92037, USA
    Cell 147:261-2. 2011
    ....
  39. ncbi request reprint A heat-sensitive TRP channel expressed in keratinocytes
    Andrea M Peier
    Genomics Institute of the Novartis Research Foundation, San Diego, CA 92121, USA
    Science 296:2046-9. 2002
    ..TRPV3 is specifically expressed in keratinocytes; hence, skin cells are capable of detecting heat via molecules similar to those in heat-sensing neurons...
  40. ncbi request reprint Noxious cold ion channel TRPA1 is activated by pungent compounds and bradykinin
    Michael Bandell
    Genomics Institute of the Novartis Research Foundation, San Diego, CA 92121, USA
    Neuron 41:849-57. 2004
    ..Collectively, these data demonstrate that TRPA1 activation elicits a painful sensation and provide a potential molecular model for why noxious cold can paradoxically be perceived as burning pain...
  41. pmc The Drosophila ortholog of vertebrate TRPA1 regulates thermotaxis
    Mark Rosenzweig
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genes Dev 19:419-24. 2005
    ..Our data suggest that thermotaxis relies upon neurons and molecules distinct from those required for high-temperature nociception. We propose dTRPA1 may control thermotaxis by sensing environmental temperature...
  42. ncbi request reprint Channeling pain
    Ardem Patapoutian
    Nat Med 12:506-7. 2006
  43. ncbi request reprint A TRP channel that senses cold stimuli and menthol
    Andrea M Peier
    Genomics Institute of the Novartis Research Foundation, San Diego, CA 92121, USA
    Cell 108:705-15. 2002
    ..Our identification of a cold-sensing TRP channel in a distinct subpopulation of sensory neurons implicates an expanded role for this family of ion channels in somatic sensory detection...
  44. ncbi request reprint Caenorhabditis elegans TRPA-1 functions in mechanosensation
    Katie S Kindt
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nat Neurosci 10:568-77. 2007
    ....

Research Grants21

  1. Somatic Sensory Neurons: Mechanisms of Heterogeneity
    Ardem Patapoutian; Fiscal Year: 2002
    ....
  2. Nociceptive Ion Channels: mechanisms of activation
    Ardem Patapoutian; Fiscal Year: 2009
    ..elegans? ..
  3. Molecular characterization of Sensory Function
    Ardem Patapoutian; Fiscal Year: 2009
    ..PUBLIC HEALTH RELEVANCE The studies described in this grant will address how ion channels involved in sensing pain and temperature are regulated. Information from these studies could potentially help in designing analgesic drugs. ..
  4. Temperature-activated TRP channels and thermosensation
    Ardem Patapoutian; Fiscal Year: 2007
    ..Here we use genetic and biochemical tools to test if TRPV3 and its close homologs play an essential role in thermosensation and pain. ..
  5. Molecular characterization of Sensory Function
    Ardem Patapoutian; Fiscal Year: 2007
    ..Finally, we will ask if these channels are required for cold detection and pain sensation in-vivo. ..
  6. Nociceptive Ion Channels: mechanisms of activation
    Ardem Patapoutian; Fiscal Year: 2006
    ..elegans? ..
  7. Molecular characterization of Sensory Function
    Ardem Patapoutian; Fiscal Year: 2006
    ..Finally, we will ask if these channels are required for cold detection and pain sensation in-vivo. ..
  8. Temperature-activated TRP channels and thermosensation
    Ardem Patapoutian; Fiscal Year: 2006
    ..Here we use genetic and biochemical tools to test if TRPV3 and its close homologs play an essential role in thermosensation and pain. ..
  9. Somatic Sensory Neurons: Mechanisms of Heterogeneity
    Ardem Patapoutian; Fiscal Year: 2003
    ....
  10. Nociceptive Ion Channels: mechanisms of activation
    Ardem Patapoutian; Fiscal Year: 2010
    ..elegans? ..
  11. Somatic Sensory Neurons: Mechanisms of Heterogeneity
    Ardem Patapoutian; Fiscal Year: 2005
    ....
  12. Temperature-activated TRP channels and thermosensation
    Ardem Patapoutian; Fiscal Year: 2005
    ..Here we use genetic and biochemical tools to test if TRPV3 and its close homologs play an essential role in thermosensation and pain. ..
  13. Nociceptive Ion Channels: mechanisms of activation
    Ardem Patapoutian; Fiscal Year: 2007
    ..elegans? ..
  14. Molecular characterization of Sensory Function
    Ardem Patapoutian; Fiscal Year: 2004
    ..Finally, we will ask if these channels are required for cold detection and pain sensation in-vivo. ..
  15. Molecular characterization of Sensory Function
    Ardem Patapoutian; Fiscal Year: 2005
    ..Finally, we will ask if these channels are required for cold detection and pain sensation in-vivo. ..
  16. Somatic Sensory Neurons: Mechanisms of Heterogeneity
    Ardem Patapoutian; Fiscal Year: 2004
    ....
  17. Temperature-activated TRP channels and thermosensation
    Ardem Patapoutian; Fiscal Year: 2009
    ..Here we use genetic and biochemical tools to test if TRPV3 and its close homologs play an essential role in thermosensation and pain. ..
  18. Molecular characterization of Sensory Function
    Ardem Patapoutian; Fiscal Year: 2010
    ..PUBLIC HEALTH RELEVANCE The studies described in this grant will address how ion channels involved in sensing pain and temperature are regulated. Information from these studies could potentially help in designing analgesic drugs. ..