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Genomes and Genes | B R OakleySummaryAffiliation: The Ohio State University Country: USA Publications
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Publications
Gamma-tubulin at ten: progress and prospectsB R Oakley
Department of Molecular Genetics, The Ohio State University, 484 W 12th Ave, Columbus, OH 43210, USA
Cell Struct Funct 24:365-72. 1999..Finally, there is evidence that gamma-tubulin has important functions in addition to microtubule nucleation, and these functions are just beginning to be investigated...- An abundance of tubulinsB R Oakley
Dept of Molecular Genetics, The Ohio State University, 484 W 12th Ave, Columbus, OH 43210, USA
Trends Cell Biol 10:537-42. 2000..delta-Tubulin has an important role in flagellar assembly in Chlamydomonas, but its role in other organisms is just beginning to be investigated, as are the functions of the recently discovered epsilon- and zeta-tubulins... - Tubulins in Aspergillus nidulansBerl R Oakley
Department of Molecular Genetics, Ohio State University, 484 West 12th Avenue, Columbus, OH 43210, USA
Fungal Genet Biol 41:420-7. 2004..gamma-Tubulin localizes to the spindle pole body and is essential for mitotic spindle formation. The phenotypes of mipA mutants suggest, moreover, that gamma-tubulin has essential functions in addition to microtubule nucleation... - Mitosis in wild-type and beta-tubulin mutant strains of Aspergillus nidulansM K Jung
Department of Molecular Genetics, The Ohio State University, 484 West 12th Avenue, Columbus, Ohio, 43210, USA
Fungal Genet Biol 24:146-60. 1998..We also often observe abnormal spindle morphologies in strains carrying benA33. Another allele, benA31, causes arrest in mitosis with short mitotic spindles and, thus, appears to inhibit spindle elongation... 
Gamma-tubulin and the C-terminal motor domain kinesin-like protein, KLPA, function in the establishment of spindle bipolarity in Aspergillus nidulansN L Prigozhina
Department of Molecular Genetics, Ohio State University, Columbus, OH 43210, USA
Mol Biol Cell 12:3161-74. 2001..These results indicate, surprisingly, that gamma-tubulin and the klpA kinesin have overlapping roles in the establishment of spindle bipolarity. We propose a model to account for these data...- Alanine-scanning mutagenesis of Aspergillus gamma-tubulin yields diverse and novel phenotypesM K Jung
Department of Molecular Genetics, The Ohio State University, Columbus, Ohio 43210, USA
Mol Biol Cell 12:2119-36. 2001..We have also placed our mutations on a model of the structure of gamma-tubulin and these data give a good initial indication of the functionally important regions of the molecule... - Gamma-tubulin is a component of the spindle pole body that is essential for microtubule function in Aspergillus nidulansB R Oakley
Department of Molecular Genetics, Ohio State University, Colombus 43210
Cell 61:1289-301. 1990..We propose that gamma-tubulin attaches microtubules to the spindle pole body, nucleates microtubule assembly, and establishes microtubule polarity in vivo... - The gamma-tubulin gene family in humansD O Wise
Department of Molecular Genetics, The Ohio State University, 484 W 12th Avenue, Columbus, Ohio 43210, USA
Genomics 67:164-70. 2000..In addition, any pharmacological agents that target one human gamma-tubulin are likely to target both... - gamma-tubulin plays an essential role in the coordination of mitotic eventsNatalie L Prigozhina
Department of Molecular Genetics, The Ohio State University, Columbus, Ohio 43210, USA
Mol Biol Cell 15:1374-86. 2004..These data reveal that gamma-tubulin has an essential role in the coordination of late mitotic events, and a microtubule-independent function in mitotic checkpoint control... - TINA interacts with the NIMA kinase in Aspergillus nidulans and negatively regulates astral microtubules during metaphase arrestAysha H Osmani
Department of Molecular Genetics, The Ohio State University, Columbus, Ohio 43210, USA
Mol Biol Cell 14:3169-79. 2003.... - In vivo analysis of the functions of gamma-tubulin-complex proteinsYi Xiong
Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA
J Cell Sci 122:4218-27. 2009..All GCPs localize normally in a GCPE deletion. We propose a model for the structure of the gamma-tubulin complex and its attachment to polar microtubule organizing centers... - The role of microtubules in rapid hyphal tip growth of Aspergillus nidulansTetsuya Horio
Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA
Mol Biol Cell 16:918-26. 2005..These data also reveal that A. nidulans exhibits a remarkable spatial regulation of microtubule disassembly within hyphal tip cells... - Identification and characterization of the asperthecin gene cluster of Aspergillus nidulansEdyta Szewczyk
Department of Molecular Genetics, Ohio State University, 484 West 12th Avenue, Columbus, Ohio 43210, USA
Appl Environ Microbiol 74:7607-12. 2008..The identification of these genes allows us to propose a biosynthetic pathway for asperthecin... - Gamma-tubulin regulates the anaphase-promoting complex/cyclosome during interphaseTania Nayak
Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA
J Cell Biol 190:317-30. 2010..Thus, gamma-tubulin functions to regulate this key mitotic and cell cycle regulatory complex... - Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens and is associated with the centrosomeY Zheng
Department of Molecular Genetics, Ohio State University, Columbus 43210 1292
Cell 65:817-23. 1991.... - Spindle formation in Aspergillus is coupled to tubulin movement into the nucleusYulia Ovechkina
Department of Molecular Genetics, The Ohio State University, Columbus 43210, USA
Mol Biol Cell 14:2192-200. 2003..nidulans. They suggest that regulation of protein movement into the nucleoplasm may be important for regulating mitotic onset in organisms with intranuclear mitosis... - The tip growth apparatus of Aspergillus nidulansNaimeh Taheri-Talesh
Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA
Mol Biol Cell 19:1439-49. 2008..Microtubules are important for delivering vesicles to the tip area and for holding the tip growth apparatus in position... - Identification and analysis of essential Aspergillus nidulans genes using the heterokaryon rescue techniqueAysha H Osmani
Department of Molecular Genetics, The Ohio State University, Columbus, Ohio 43210, USA
Nat Protoc 1:2517-26. 2006..Diagnostic PCR can be used to confirm deletions at the molecular level. This technique is suitable for large-scale gene-deletion programs and can be completed within 3 weeks... - Fusion PCR and gene targeting in Aspergillus nidulansEdyta Szewczyk
Department of Molecular Genetics, Ohio State University, 484 West 12th Avenue, Columbus, Ohio 43210, USA
Nat Protoc 1:3111-20. 2006..nidulans with fusion PCR products results in high frequencies of accurate gene targeting. Fusion PCR takes less than 2 d. Protoplast formation and transformation takes less than 1 d... - Cytoplasmic dynein's mitotic spindle pole localization requires a functional anaphase-promoting complex, gamma-tubulin, and NUDF/LIS1 in Aspergillus nidulansShihe Li
Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
Mol Biol Cell 16:3591-605. 2005.... - Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzaeJames E Galagan
The Broad Institute of MIT and Harvard, 320 Charles Street, Cambridge, Massachusetts 02142, USA
Nature 438:1105-15. 2005..These results enhance our understanding of these widely studied fungi as well as provide new insight into eukaryotic genome evolution and gene regulation... 
Sumoylation in Aspergillus nidulans: sumO inactivation, overexpression and live-cell imagingKoon Ho Wong
Department of Genetics, The University of Melbourne, Grattan Street, Parkville, VIC 3010, Australia
Fungal Genet Biol 45:728-37. 2008..Localization of GFP-SumO is dynamic, accumulating in punctate spots within the nucleus during interphase, lost at the onset of mitosis and re-accumulating during telophase...- Molecular genetic mining of the Aspergillus secondary metabolome: discovery of the emericellamide biosynthetic pathwayYi Ming Chiang
Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA 90089, USA
Chem Biol 15:527-32. 2008..From the sequences of the genes, we are able to deduce a biosynthetic pathway for the emericellamides. The identification of this biosynthetic pathway opens the door to engineering novel analogs of this structurally complex metabolite... - Expression of Arabidopsis gamma-tubulin in fission yeast reveals conserved and novel functions of gamma-tubulinTetsuya Horio
Department of Nutrition, The University of Tokushima School of Medicine, Kuramoto, Tokushima, 770 8503, Japan
Plant Physiol 133:1926-34. 2003.... - Microtubule organization requires cell cycle-dependent nucleation at dispersed cytoplasmic sites: polar and perinuclear microtubule organizing centers in the plant pathogen Ustilago maydisAnne Straube
, Karl-von-Frisch-Strabetae, D-35043 Marburg, Germany
Mol Biol Cell 14:642-57. 2003..Taken together, our data suggest that MT reorganization in U. maydis depends on cell cycle-specific nucleation at dispersed cytoplasmic sites, at a polar MTOC and the SPB... - Dual-Color imaging of nuclear division and mitotic spindle elongation in live cells of Aspergillus nidulansWenqi Su
Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
Eukaryot Cell 3:553-6. 2004..This system allows new details of mitosis and nuclear movement to be revealed...