Dolores D Mruk

Summary

Affiliation: The Rockefeller University
Country: USA

Publications

  1. doi The myotubularin family of lipid phosphatases in disease and in spermatogenesis
    Dolores D Mruk
    Center for Biomedical Research, New York, NY 10065, USA
    Biochem J 433:253-62. 2011
  2. ncbi Sertoli-Sertoli and Sertoli-germ cell interactions and their significance in germ cell movement in the seminiferous epithelium during spermatogenesis
    Dolores D Mruk
    Population Council, Center for Biomedical Research, New York, New York 10021, USA
    Endocr Rev 25:747-806. 2004
  3. doi Delivering non-hormonal contraceptives to men: advances and obstacles
    Dolores D Mruk
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Trends Biotechnol 26:90-9. 2008
  4. ncbi Crosstalk between Rab GTPases and cell junctions
    Dolores D Mruk
    Population Council, Center for Biomedical Research 1230 York Ave, New York, NY 10021, USA
    Contraception 72:280-90. 2005
  5. pmc Anchoring junctions as drug targets: role in contraceptive development
    Dolores D Mruk
    Population Council, Center for Biomedical Research, The Mary M Wohlford Laboratory for Male Contraceptive Research, 1230 York Avenue, New York, NY 10065, USA
    Pharmacol Rev 60:146-80. 2008
  6. ncbi A male contraceptive targeting germ cell adhesion
    Dolores D Mruk
    Population Council, Center for Biomedical Research, 1230 York Avenue, New York, New York 10021, USA
    Nat Med 12:1323-8. 2006
  7. ncbi Regulation of ectoplasmic specialization dynamics in the seminiferous epithelium by focal adhesion-associated proteins in testosterone-suppressed rat testes
    Ching Hang Wong
    Population Council, Center for Biomedical Research, New York, New York 10021, USA
    Endocrinology 146:1192-204. 2005
  8. pmc P-glycoprotein regulates blood-testis barrier dynamics via its effects on the occludin/zonula occludens 1 (ZO-1) protein complex mediated by focal adhesion kinase (FAK)
    Linlin Su
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Population Council, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 108:19623-8. 2011
  9. ncbi Tumor necrosis factor {alpha} reversibly disrupts the blood-testis barrier and impairs Sertoli-germ cell adhesion in the seminiferous epithelium of adult rat testes
    Michelle W M Li
    Population Council, 1230 York Avenue, New York 10021, USA
    J Endocrinol 190:313-29. 2006
  10. ncbi Regulation of Sertoli-germ cell adherens junction dynamics via changes in protein-protein interactions of the N-cadherin-beta-catenin protein complex which are possibly mediated by c-Src and myotubularin-related protein 2: an in vivo study using an androg
    Jiayi Zhang
    Population Council, Center for Biomedical Research, New York, New York 10021, USA
    Endocrinology 146:1268-84. 2005

Collaborators

Detail Information

Publications100

  1. doi The myotubularin family of lipid phosphatases in disease and in spermatogenesis
    Dolores D Mruk
    Center for Biomedical Research, New York, NY 10065, USA
    Biochem J 433:253-62. 2011
    ....
  2. ncbi Sertoli-Sertoli and Sertoli-germ cell interactions and their significance in germ cell movement in the seminiferous epithelium during spermatogenesis
    Dolores D Mruk
    Population Council, Center for Biomedical Research, New York, New York 10021, USA
    Endocr Rev 25:747-806. 2004
    ..Other factors such as proteases, protease inhibitors, GTPases, kinases, and phosphatases also come into play. In this review, we focus on this cellular phenomenon, recapping recent developments in the field...
  3. doi Delivering non-hormonal contraceptives to men: advances and obstacles
    Dolores D Mruk
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Trends Biotechnol 26:90-9. 2008
    ..In this article, we highlight the latest developments regarding contraceptive delivery to men and with the aim of providing useful information for investigators in future studies...
  4. ncbi Crosstalk between Rab GTPases and cell junctions
    Dolores D Mruk
    Population Council, Center for Biomedical Research 1230 York Ave, New York, NY 10021, USA
    Contraception 72:280-90. 2005
    ..In this mini-review, we provide an overview of Rab GTPase structure, function, and regulation, while placing added emphasis on the role of Rabs in cell junction dynamics in the testis...
  5. pmc Anchoring junctions as drug targets: role in contraceptive development
    Dolores D Mruk
    Population Council, Center for Biomedical Research, The Mary M Wohlford Laboratory for Male Contraceptive Research, 1230 York Avenue, New York, NY 10065, USA
    Pharmacol Rev 60:146-80. 2008
    ..Herein, we discuss a novel approach with the aim of developing a nonhormonal male contraceptive for future human use, one that involves perturbing adhesion between Sertoli and germ cells in the testis...
  6. ncbi A male contraceptive targeting germ cell adhesion
    Dolores D Mruk
    Population Council, Center for Biomedical Research, 1230 York Avenue, New York, New York 10021, USA
    Nat Med 12:1323-8. 2006
    ..5 microg Adjudin per kg b.w. was administered intraperitoneally, which was similar to results when 50 mg per kg b.w. was given orally. This represents a substantial increase in Adjudin's selectivity and efficacy as a male contraceptive...
  7. ncbi Regulation of ectoplasmic specialization dynamics in the seminiferous epithelium by focal adhesion-associated proteins in testosterone-suppressed rat testes
    Ching Hang Wong
    Population Council, Center for Biomedical Research, New York, New York 10021, USA
    Endocrinology 146:1192-204. 2005
    ..In addition to its role in conferring cell adhesion formation, the p-FAK/c-Src protein complex apparently also regulates apical ES disruption via the ERK signaling pathway...
  8. pmc P-glycoprotein regulates blood-testis barrier dynamics via its effects on the occludin/zonula occludens 1 (ZO-1) protein complex mediated by focal adhesion kinase (FAK)
    Linlin Su
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Population Council, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 108:19623-8. 2011
    ..These findings indicate that P-glycoprotein is involved in BTB restructuring during spermatogenesis but that P-glycoprotein-mediated restructuring does not "open up" the BTB to make it freely permeable to drugs...
  9. ncbi Tumor necrosis factor {alpha} reversibly disrupts the blood-testis barrier and impairs Sertoli-germ cell adhesion in the seminiferous epithelium of adult rat testes
    Michelle W M Li
    Population Council, 1230 York Avenue, New York 10021, USA
    J Endocrinol 190:313-29. 2006
    ....
  10. ncbi Regulation of Sertoli-germ cell adherens junction dynamics via changes in protein-protein interactions of the N-cadherin-beta-catenin protein complex which are possibly mediated by c-Src and myotubularin-related protein 2: an in vivo study using an androg
    Jiayi Zhang
    Population Council, Center for Biomedical Research, New York, New York 10021, USA
    Endocrinology 146:1268-84. 2005
    ..In summary, these results illustrate that the cadherin/catenin is a crucial cell adhesion complex that regulates AJ dynamics in the testis, and its functionality is likely modulated by the MTMR2/c-Src protein complex...
  11. ncbi Dynamin II interacts with the cadherin- and occludin-based protein complexes at the blood-testis barrier in adult rat testes
    Pearl P Y Lie
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, New York 10021, USA
    J Endocrinol 191:571-86. 2006
    ..Thus, this may permit the occludin/ZO-1 complexes to maintain the BTB integrity when the cadherin/catenin complexes are dissociated to facilitate germ cell movement...
  12. ncbi Regulation of blood-testis barrier dynamics: an in vivo study
    Ching Hang Wong
    Population Council, Center for Biomedical Research, 1230 York Avenue, New York, NY 10021, USA
    J Cell Sci 117:783-98. 2004
    ....
  13. ncbi Adjudin-mediated junction restructuring in the seminiferous epithelium leads to displacement of soluble guanylate cyclase from adherens junctions
    Oli Sarkar
    Population Council, Center for Biomedical Research, New York, New York 10021, USA
    J Cell Physiol 208:175-87. 2006
    ..e., occludin and ZO-1) decreased. Taken collectively, these results suggest that sGC participates in the remodeling of cell junctions during spermatogenesis...
  14. pmc Epidermal growth factor receptor pathway substrate 8 (Eps8) is a novel regulator of cell adhesion and the blood-testis barrier integrity in the seminiferous epithelium
    Pearl P Y Lie
    Center for Biomedical Research, Population Council, 1230 York Ave, New York, NY 10065, USA
    FASEB J 23:2555-67. 2009
    ....
  15. doi p-FAK-Tyr(397) regulates spermatid adhesion in the rat testis via its effects on F-actin organization at the ectoplasmic specialization
    Hin Ting Wan
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, New York, New York
    Am J Physiol Endocrinol Metab 305:E687-99. 2013
    ..These findings illustrate a mechanistic pathway mediated by p-FAK-Tyr(397) that regulates spermatid adhesion at the apical ES in vivo...
  16. pmc A peptide derived from laminin-γ3 reversibly impairs spermatogenesis in rats
    Linlin Su
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, 1230 York Avenue, New York, New York 10065, USA
    Nat Commun 3:1185. 2012
    ..The impairment of spermatogenesis suggests that this laminin peptide fragment may serve as a contraceptive in male rats...
  17. ncbi C-Src and c-Yes are two unlikely partners of spermatogenesis and their roles in blood-testis barrier dynamics
    Xiang Xiao
    Center for Biomedical Research, Population Council, New York New York, USA
    Adv Exp Med Biol 763:295-317. 2012
    ..We also propose a hypothetical model on the mechanistic functions of c-Src and c-Yes in spermatogenesis so that functional experiments can be designed in future studies...
  18. pmc Focal adhesion kinase-Tyr407 and -Tyr397 exhibit antagonistic effects on blood-testis barrier dynamics in the rat
    Pearl P Y Lie
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 109:12562-7. 2012
    ....
  19. pmc Unraveling the molecular targets pertinent to junction restructuring events during spermatogenesis using the Adjudin-induced germ cell depletion model
    Weiliang Xia
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, New York 10021, USA
    J Endocrinol 192:563-83. 2007
    ....
  20. pmc An autocrine axis in the testis that coordinates spermiation and blood-testis barrier restructuring during spermatogenesis
    Helen H N Yan
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 105:8950-5. 2008
    ....
  21. pmc Rai14 (retinoic acid induced protein 14) is involved in regulating f-actin dynamics at the ectoplasmic specialization in the rat testis*
    Xiaojing Qian
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, New York, NY, USA
    PLoS ONE 8:e60656. 2013
    ..In short, Rai14 is involved in the re-organization of actin filaments in Sertoli cells during the epithelial cycle, participating in conferring spermatid polarity and cell adhesion in the testis...
  22. pmc Disruption of the blood-testis barrier integrity by bisphenol A in vitro: is this a suitable model for studying blood-testis barrier dynamics?
    Michelle W M Li
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Int J Biochem Cell Biol 41:2302-14. 2009
    ..In summary, bisphenol A reversibly perturbs the integrity of the blood-testis barrier in Sertoli cells in vitro, which can also serve as a suitable model for studying the dynamics of the blood-testis barrier...
  23. ncbi Cellular localization of sphingomyelin synthase 2 in the seminiferous epithelium of adult rat testes
    Nikki P Y Lee
    Population Council, Center for Biomedical Research, New York, NY 10021, USA
    J Endocrinol 192:17-32. 2007
    ..Collectively, these results suggest that SMS2 may play a crucial role in the lipid metabolism in acrosome formation and the plasma membrane restructuring from late round spermatids to early elongating spermatids...
  24. pmc Differential effects of testosterone and TGF-β3 on endocytic vesicle-mediated protein trafficking events at the blood-testis barrier
    Linlin Su
    Center for Biomedical Research, Population Council, New York, NY 10065, USA
    Exp Cell Res 316:2945-60. 2010
    ....
  25. doi RAB13 participates in ectoplasmic specialization dynamics in the rat testis
    Dolores D Mruk
    Center for Biomedical Research, Population Council, New York, New York 10065, USA
    Biol Reprod 80:590-601. 2009
    ..Taken collectively, these results suggest that RAB13 functions in ectoplasmic specialization dynamics in the testis...
  26. pmc Targeted and reversible disruption of the blood-testis barrier by an FSH mutant-occludin peptide conjugate
    Ching Hang Wong
    Center for Biomedical Research, Population Council, 1230 York Ave, New York, NY 10021, USA
    FASEB J 21:438-48. 2007
    ..In summary, the use of an FSH mutant-occludin peptide conjugate is a feasible nanodevice to transiently compromise the BTB...
  27. pmc Regulation of actin dynamics and protein trafficking during spermatogenesis--insights into a complex process
    Wenhui Su
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, New York, NY 10065, USA
    Crit Rev Biochem Mol Biol 48:153-72. 2013
    ..It is obvious that this model will be rapidly updated when more data are available in future years...
  28. pmc Palladin is a regulator of actin filament bundles at the ectoplasmic specialization in adult rat testes
    Xiaojing Qian
    Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, 1230 York Ave, New York, New York 10065, USA
    Endocrinology 154:1907-20. 2013
    ..Its knockdown in vivo also perturbed F-actin organization that led to a loss of spermatid polarity and adhesion, causing defects in spermatid transport and spermiation. In summary, palladin is an actin filament regulator at the ES...
  29. ncbi Role of P-glycoprotein at the blood-testis barrier on adjudin distribution in the testis: a revisit of recent data
    Linlin Su
    Center for Biomedical Research, Population Council, New York, New York, USA
    Adv Exp Med Biol 763:318-33. 2012
    ..Herein, we summarize latest findings in this area of research, we also provide a critical evaluation on research areas that deserve attention in future studies...
  30. pmc Focal adhesion kinase is a blood-testis barrier regulator
    Erica R Siu
    Center for Biomedical Research, Population Council, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 106:9298-303. 2009
    ..Furthermore, FAK was shown to be a molecular target of cadmium because its knockdown would desensitize Sertoli cells to cadmium-induced TJ barrier disruption. In summary, FAK is a unique regulator of BTB dynamics in the testis...
  31. pmc Regulation of blood-testis barrier dynamics by TGF-beta3 is a Cdc42-dependent protein trafficking event
    Elissa W P Wong
    Center for Biomedical Research, Population Council, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 107:11399-404. 2010
    ..In summary, Cdc42 is a crucial regulatory component in the TGF-beta3-mediated cascade of events that leads to the disruption of the TJ fibrils above the preleptotene spermatocytes to facilitate their transit...
  32. pmc c-Yes regulates cell adhesion at the blood-testis barrier and the apical ectoplasmic specialization in the seminiferous epithelium of rat testes
    Xiang Xiao
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Int J Biochem Cell Biol 43:651-65. 2011
    ..In summary, c-Yes regulates BTB and apical ES integrity by maintaining proper distribution of integral membrane proteins and actin filament organization at these sites...
  33. pmc Biology and regulation of ectoplasmic specialization, an atypical adherens junction type, in the testis
    Elissa W P Wong
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Biochim Biophys Acta 1778:692-708. 2008
    ..g., apical ES) in the testis. The biology and regulation based on recent studies in the testis are of interest to cell biologists in the field, in particular their regulation, which perhaps is applicable to tumorigenesis...
  34. ncbi Interactions among IQGAP1, Cdc42, and the cadherin/catenin protein complex regulate Sertoli-germ cell adherens junction dynamics in the testis
    Wing Yee Lui
    Population Council, Center for Biomedical Research, New York, New York 10021, USA
    J Cell Physiol 202:49-66. 2005
    ..These results illustrate that the interactions among IQGAP1, Cdc42, and beta-catenin are crucial to the regulation of Sertoli-germ cell, but not Sertoli-Sertoli cell, AJ dynamics in the seminiferous epithelium...
  35. ncbi Differential interactions between transforming growth factor-beta3/TbetaR1, TAB1, and CD2AP disrupt blood-testis barrier and Sertoli-germ cell adhesion
    Weiliang Xia
    Population Council, Center for Biomedical Research, 1230 York Avenue, New York, NY 10021, USA
    J Biol Chem 281:16799-813. 2006
    ..However, TGF-beta3 selectively disrupts Sertoli-germ cell adhesion in the seminiferous epithelium to facilitate germ cell migration without compromising BTB when TbetaRI interacts only with adaptor CD2AP...
  36. pmc Breast cancer resistance protein regulates apical ectoplasmic specialization dynamics stage specifically in the rat testis
    Xiaojing Qian
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, New York, NY 10065, USA
    Am J Physiol Endocrinol Metab 304:E757-69. 2013
    ..Indeed, a knockdown of Bcrp by RNAi in the testis perturbed the apical ES function, disrupting spermatid polarity and adhesion. In summary, Bcrp is a regulator of the F-actin-rich apical ES in the testis...
  37. pmc c-Yes regulates cell adhesion at the apical ectoplasmic specialization-blood-testis barrier axis via its effects on protein recruitment and distribution
    Xiang Xiao
    Center for Biomedical Research, Population Council, New York, NY 10065, USA
    Am J Physiol Endocrinol Metab 304:E145-59. 2013
    ..g., nectin-3) and possibly the result of changes in the underlying actin filaments at the BTB and the apical ES. These findings implicate that c-Yes is a likely target of male contraceptive research...
  38. pmc Intercellular adhesion molecule-2 is involved in apical ectoplasmic specialization dynamics during spermatogenesis in the rat
    Xiang Xiao
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    J Endocrinol 216:73-86. 2013
    ..Taken collectively, these results illustrate that ICAM2 plays an important role in apical ES dynamics during spermatogenesis...
  39. pmc The Scribble/Lgl/Dlg polarity protein complex is a regulator of blood-testis barrier dynamics and spermatid polarity during spermatogenesis
    Wenhui Su
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, New York, NY 10065, USA
    Endocrinology 153:6041-53. 2012
    ..In summary, the Scribble/Lgl/Dlg complex participates in the regulation of BTB dynamics and spermatid adhesion/polarity in the testis...
  40. pmc Rictor/mTORC2 regulates blood-testis barrier dynamics via its effects on gap junction communications and actin filament network
    Ka Wai Mok
    Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, 1230 York Ave, New York, NY 10065, USA
    FASEB J 27:1137-52. 2013
    ..Rictor knockdown by RNAi was also found to impede Sertoli cell-cell GJ communication, disrupting protein distribution (e.g., occludin, ZO-1) at the BTB, illustrating that rictor is a crucial BTB regulator...
  41. ncbi Polarity protein complex Scribble/Lgl/Dlg and epithelial cell barriers
    Wen Hui Su
    Center for Biomedical Research, Population Council, New York New York, USA
    Adv Exp Med Biol 763:149-70. 2012
    ....
  42. ncbi The apical ectoplasmic specialization-blood-testis barrier functional axis is a novel target for male contraception
    Ka Wai Mok
    Center for Biomedical Research, Population Council, New York, New York, USA
    Adv Exp Med Biol 763:334-55. 2012
    ....
  43. pmc Crosstalk between desmoglein-2/desmocollin-2/Src kinase and coxsackie and adenovirus receptor/ZO-1 protein complexes, regulates blood-testis barrier dynamics
    Pearl P Y Lie
    Population Council, Center for Biomedical Research, 1230 York Avenue, New York, NY 10065, United States
    Int J Biochem Cell Biol 42:975-86. 2010
    ..We hereby propose a novel regulatory protein complex composed of desmoglein-2, desmocollin-2, c-Src, coxsackie and adenovirus receptor and zonula occludens-1 at the blood-testis barrier...
  44. pmc Drug transporter, P-glycoprotein (MDR1), is an integrated component of the mammalian blood-testis barrier
    Linlin Su
    Population Council, Center for Biomedical Research, 1230 York Avenue, NY 10065, USA
    Int J Biochem Cell Biol 41:2578-87. 2009
    ..g., occludin, claudin-11 and JAM-A) was noted when testis lysates from Adjudin-treated rats were used for co-immunoprecipitation experiments, suggesting that P-gp may enhance BTB function during Sertoli-germ cell junction restructuring...
  45. pmc Connexin 43 and plakophilin-2 as a protein complex that regulates blood-testis barrier dynamics
    Michelle W M Li
    Center for Biomedical Research, Population Council, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 106:10213-8. 2009
    ....
  46. ncbi Zyxin, axin, and Wiskott-Aldrich syndrome protein are adaptors that link the cadherin/catenin protein complex to the cytoskeleton at adherens junctions in the seminiferous epithelium of the rat testis
    Nikki P Y Lee
    Population Council, New York, New York 10021, USA
    J Androl 25:200-15. 2004
    ..Collectively, these results illustrate that while these adaptors are structurally associated with the cadherin/catenin complex in the testis, they are regulated differently...
  47. pmc Par3/Par6 polarity complex coordinates apical ectoplasmic specialization and blood-testis barrier restructuring during spermatogenesis
    Elissa W P Wong
    Population Council, 1230 York Avenue, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 105:9657-62. 2008
    ....
  48. pmc Intercellular adhesion molecules (ICAMs) and spermatogenesis
    Xiang Xiao
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Hum Reprod Update 19:167-86. 2013
    ..However, the regulatory molecules that co-ordinate cellular events in this axis are not known...
  49. pmc Connexin 43 is critical to maintain the homeostasis of the blood-testis barrier via its effects on tight junction reassembly
    Michelle W M Li
    Center for Biomedical Research, Population Council, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 107:17998-8003. 2010
    ..In summary, Cx43 is crucial for TJ reassembly at the BTB during its cyclic restructuring throughout the seminiferous epithelial cycle of spermatogenesis...
  50. pmc rpS6 Regulates blood-testis barrier dynamics by affecting F-actin organization and protein recruitment
    Ka Wai Mok
    Center for Biomedical Research, Population Council, New York, New York 10065, USA
    Endocrinology 153:5036-48. 2012
    ..g. claudin-11 and occludin) to the BTB. Thus, rpS6 in the mTORC1 pathway regulates BTB restructuring via its effects on the F-actin organization and protein recruitment at the BTB...
  51. ncbi Fer kinase/FerT and adherens junction dynamics in the testis: an in vitro and in vivo study
    Yong Mei Chen
    Population Council, New York, New York, 10021, USA
    Biol Reprod 69:656-72. 2003
    ..In summary, Fer kinase structurally associates with the N-cadherin/catenin protein complex in the testis and can possibly be used to mediate signaling function via the cadherin/catenin protein complex...
  52. doi Adjudin, a potential male contraceptive, exerts its effects locally in the seminiferous epithelium of mammalian testes
    Ka Wai Mok
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, 1230 York Avenue, New York, New York 10065, USA
    Reproduction 141:571-80. 2011
    ..In this review, we highlight some of the recent advances and obstacles regarding the possible use of adjudin as a male contraceptive...
  53. pmc Regulation of spermatogenesis in the microenvironment of the seminiferous epithelium: new insights and advances
    C Yan Cheng
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Mol Cell Endocrinol 315:49-56. 2010
    ..These findings provide a new window of research for investigators in the field to tackle the functional regulation of spermatogenesis...
  54. ncbi Role of tissue inhibitor of metalloproteases-1 in junction dynamics in the testis
    Dolores D Mruk
    Population Council, Center for Biomedical Research, New York, New York 10021, USA
    J Androl 24:510-23. 2003
    ....
  55. pmc C-type natriuretic peptide regulates blood-testis barrier dynamics in adult rat testes
    Weiliang Xia
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10021
    Proc Natl Acad Sci U S A 104:3841-6. 2007
    ..In summary, CNP secreted by Sertoli and germ cells into the BTB microenvironment regulates BTB dynamics during spermatogenesis...
  56. pmc Coxsackie and adenovirus receptor (CAR) is a product of Sertoli and germ cells in rat testes which is localized at the Sertoli-Sertoli and Sertoli-germ cell interface
    Claire Q F Wang
    Center for Biomedical Research, The Population Council, 1230 York Avenue, New York, NY 10021, USA
    Exp Cell Res 313:1373-92. 2007
    ..It is possible that the receptor utilized by viral pathogens to breakthrough the epithelial barrier was also employed by developing germ cells to migrate through the inter-Sertoli cell junctions...
  57. pmc TGF-beta3 and TNFalpha perturb blood-testis barrier (BTB) dynamics by accelerating the clathrin-mediated endocytosis of integral membrane proteins: a new concept of BTB regulation during spermatogenesis
    Weiliang Xia
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Dev Biol 327:48-61. 2009
    ..In summary, this report demonstrates for the first time that BTB dynamics are regulated by TGF-beta3 and TNFalpha via an enhancement of protein endocytosis at the BTB...
  58. ncbi Regulation of Sertoli-germ cell adherens junction dynamics in the testis via the nitric oxide synthase (NOS)/cGMP/protein kinase G (PRKG)/beta-catenin (CATNB) signaling pathway: an in vitro and in vivo study
    Nikki P Y Lee
    Population Council, New York, New York 10021, USA
    Biol Reprod 73:458-71. 2005
    ..These results illustrate that the CDH2/CATNB-mediated adhesion function in the testis is regulated, at least in part, via the NOS/cGMP/PRKG/CATNB pathway...
  59. pmc An occludin-focal adhesion kinase protein complex at the blood-testis barrier: a study using the cadmium model
    Erica R Siu
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, New York 10065, USA
    Endocrinology 150:3336-44. 2009
    ..Thus, these findings demonstrate that FAK is an integrated regulatory component of the occludin-ZO-1 protein complex, suggesting that functional studies can be performed to study the role of FAK in BTB dynamics...
  60. pmc Regulation of blood-testis barrier dynamics by focal adhesion kinase (FAK): an unexpected turn of events
    C Yan Cheng
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, New York, NY, USA
    Cell Cycle 8:3493-9. 2009
    ..Lastly, we describe a working model, which can be used to design future functional experiments to explore the involvement of FAK in BTB dynamics by investigators in the field...
  61. pmc Ectoplasmic specialization: a friend or a foe of spermatogenesis?
    Helen H N Yan
    Center for Biomedical Research, Population Council, New York, NY 10021, USA
    Bioessays 29:36-48. 2007
    ..We discuss herein the mechanisms that maintain the cross-talk between ES and blood-testis barrier to facilitate cell movement and orientation in the seminiferous epithelium...
  62. pmc Cytoskeletal dynamics and spermatogenesis
    Pearl P Y Lie
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Philos Trans R Soc Lond B Biol Sci 365:1581-92. 2010
    ....
  63. pmc Restricted Arp3 expression in the testis prevents blood-testis barrier disruption during junction restructuring at spermatogenesis
    Pearl P Y Lie
    Center for Biomedical Research, Population Council, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 107:11411-6. 2010
    ....
  64. pmc Filamin A is a regulator of blood-testis barrier assembly during postnatal development in the rat testis
    Wenhui Su
    Population Council, New York, New York 10065, USA
    Endocrinology 153:5023-35. 2012
    ..In short, filamin A participates in BTB assembly by regulating protein recruitment during postnatal development in the rat testis...
  65. doi Drug transporters and blood--testis barrier function
    Linlin Su
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, New York, New York 10065, USA
    J Endocrinol 209:337-51. 2011
    ....
  66. pmc Interleukin-1alpha is a regulator of the blood-testis barrier
    Pearl P Y Lie
    Center for Biomedical Research, Population Council, New York, NY 10065, USA
    FASEB J 25:1244-53. 2011
    ..As important, an increase in the kinetics of occludin internalization but a decrease in its rate of degradation was noted following IL-1α treatment. These results indicate that IL-1α is a critical regulator of BTB dynamics...
  67. ncbi Adhering junction dynamics in the testis are regulated by an interplay of beta 1-integrin and focal adhesion complex-associated proteins
    Michelle K Y Siu
    The Population Council, Center for Biomedical Research, New York, New York 10021, USA
    Endocrinology 144:2141-63. 2003
    ....
  68. doi Cross-talk between tight and anchoring junctions-lesson from the testis
    Helen H N Yan
    Center for Biomedical Research, The Population Council, New York, NY 10021, USA
    Adv Exp Med Biol 636:234-54. 2008
    ..Herein, we provide a critical review based on the recently available data regarding the cross-talk between TJ and anchoring junction to allow simultaneous maintenance of the BTB and germ cell movement across the seminiferous epithelium...
  69. pmc Testicular cell junction: a novel target for male contraception
    Nikki P Y Lee
    Center for Biomedical Research, Population Council, New York, New York 10065, USA
    Curr Med Chem 16:906-15. 2009
    ..While it will still take much time and effort of intensive investigation before a product can reach the consumable market, these findings have provided hope for better family planning involving men...
  70. ncbi Cytokines and junction restructuring during spermatogenesis--a lesson to learn from the testis
    Weiliang Xia
    Population Council, Center for Biomedical Research, New York, NY 10021, USA
    Cytokine Growth Factor Rev 16:469-93. 2005
    ..Furthermore, a molecular model is presented regarding how cytokines selectively regulate TJ/AJ restructuring in the epithelium during spermatogenesis...
  71. ncbi Alpha2-macroglobulin expression in the liver in response to inflammation is mediated by the testis
    Wing Yee Lui
    Population Council, Center for Biomedical Research, 1230 York Avenue, New York, New York 10021, USA
    J Endocrinol 185:497-505. 2005
    ....
  72. ncbi Junction restructuring and spermatogenesis: the biology, regulation, and implication in male contraceptive development
    Helen H N Yan
    Center for Biomedical Research, Population Council, New York, New York 10021, USA
    Curr Top Dev Biol 80:57-92. 2008
    ..Besides, we highlight important areas of research that can now be undertaken, and functional studies that can be designed to tackle different issues pertinent to junction restructuring during spermatogenesis...
  73. ncbi Rab4A GTPase catenin interactions are involved in cell junction dynamics in the testis
    Dolores D Mruk
    Population Council, Center for Biomedical Research, 1230 York Ave, New York, NY 10021, USA
    J Androl 28:742-54. 2007
    ..Taken collectively, these results suggest that Rab4A participates in adherens junction dynamics...
  74. pmc Coordinating cellular events during spermatogenesis: a biochemical model
    Pearl P Y Lie
    Center for Biomedical Research, New York, NY 10065, USA
    Trends Biochem Sci 34:366-73. 2009
    ..In this way, MAPKs would synchronize cell cycle progression in primary spermatocytes with junction remodeling and cell migration across the BTB...
  75. pmc An in vivo study on adjudin and blood-testis barrier dynamics
    Ilona A Kopera
    Population Council, Center for Biomedical Research, New York, New York 10065, USA
    Endocrinology 150:4724-33. 2009
    ..Collectively these results demonstrate that adjudin targets the apical ES as well as the basal ES and tight junction, which in turn delays assembly of the BTB...
  76. pmc Cytokines and junction restructuring events during spermatogenesis in the testis: an emerging concept of regulation
    Michelle W M Li
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, United States
    Cytokine Growth Factor Rev 20:329-38. 2009
    ..Possible regulators that mediate cytokine-induced junction restructuring, including gap junction and extracellular matrix, and the role of testosterone on junction dynamics in the testis will also be discussed...
  77. ncbi Blood-testis barrier dynamics are regulated by {alpha}2-macroglobulin via the c-Jun N-terminal protein kinase pathway
    Ching Hang Wong
    Population Council, 1230 York Avenue, New York, New York 10021, USA
    Endocrinology 146:1893-908. 2005
    ..This finding illustrates for the first time that a cell-matrix restructuring event occurs in normal cell physiology at the cell-cell interface in the testis, highlighting the significance of alpha(2)-MG in the regulation of BTB function...
  78. ncbi Rab8B GTPase and junction dynamics in the testis
    Ann S N Lau
    Population Council, Center for Biomedical Research, New York, New York 10021, USA
    Endocrinology 144:1549-63. 2003
    ..Taken collectively, these studies suggest that Rab8B participates in adherens junction dynamics in the testis...
  79. doi The biology of the desmosome-like junction a versatile anchoring junction and signal transducer in the seminiferous epithelium
    Pearl P Y Lie
    Population Council, Center for Biomedical Research, New York, New York, USA
    Int Rev Cell Mol Biol 286:223-69. 2011
    ..We place special emphasis on the emerging role of desmosome-like junctions as signal transducers during germ cell movement across the seminiferous epithelium...
  80. pmc Sertoli-germ cell junctions in the testis: a review of recent data
    Ilona A Kopera
    Population Council, Center for Biomedical Research, 1230 York Avenue, New York, NY 10065, USA
    Philos Trans R Soc Lond B Biol Sci 365:1593-605. 2010
    ..Here, we discuss recent findings relating to these two junction types in the testis, highlighting prospective areas that should be investigated in future studies...
  81. doi Drug transporters, the blood-testis barrier, and spermatogenesis
    Linlin Su
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, New York, New York 10065, USA
    J Endocrinol 208:207-23. 2011
    ..For example, when drugs (e.g. male contraceptives) that can perturb germ cell adhesion and/or maturation are actively pumped out of the testis or are prevented from entering the apical compartment, such as by efflux pumps...
  82. pmc Blood-testis barrier dynamics are regulated by testosterone and cytokines via their differential effects on the kinetics of protein endocytosis and recycling in Sertoli cells
    Helen H N Yan
    Center for Biomedical Research, Population Council, New York, New York 10065, USA
    FASEB J 22:1945-59. 2008
    ..The net result of these interactions, in turn, determines the steady-state protein levels at the Sertoli-Sertoli cell interface at the BTB...
  83. ncbi Cell junction dynamics in the testis: Sertoli-germ cell interactions and male contraceptive development
    C Yan Cheng
    Population Council, Center for Biomedical Research, New York, New York 10021, USA
    Physiol Rev 82:825-74. 2002
    ..An in-depth survey in this subject has also identified several potential targets to be tackled to perturb spermatogenesis, which will likely lead to the development of novel male contraceptives...
  84. ncbi TGF-beta3 regulates the blood-testis barrier dynamics via the p38 mitogen activated protein (MAP) kinase pathway: an in vivo study
    Wing Yee Lui
    Population Council, New York, New York 10021, USA
    Endocrinology 144:1139-42. 2003
    ..These results illustrate that BTB dynamics in vivo are regulated by the TGF-beta3/p38 MAP kinase pathway, which in turn determines the level of occludin at the site of Sertoli cells TJs...
  85. pmc Interleukin 1 alpha (IL1A) is a novel regulator of the blood-testis barrier in the rat
    Oli Sarkar
    Population Council, Center for Biomedical Research, New York, NY 10065, USA
    Biol Reprod 78:445-54. 2008
    ..It is possible that localized production of IL1A by Sertoli and/or germ cells in vivo results in BTB restructuring, and this may facilitate the movement of leptotene spermatocytes across the BTB...
  86. pmc Cadmium-induced testicular injury
    Erica R Siu
    Center for Biomedical Research, Population Council, New York, NY 10065, USA
    Toxicol Appl Pharmacol 238:240-9. 2009
    ..Furthermore, some of the recent studies have shed lights on potential therapeutic or preventive approaches that can be developed in future studies by blocking or minimizing the destructive effects of Cd to testicular function in men...
  87. ncbi Myotubularin phosphoinositide phosphatases, protein phosphatases, and kinases: their roles in junction dynamics and spermatogenesis
    Jiayi Zhang
    Population Council, Center for Biomedical Research, New York, New York, USA
    J Cell Physiol 204:470-83. 2005
    ..Specific research areas that are open for further investigation are also highlighted...
  88. pmc The biology of spermatogenesis: the past, present and future
    C Yan Cheng
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Philos Trans R Soc Lond B Biol Sci 365:1459-63. 2010
    ....
  89. ncbi Antioxidant superoxide dismutase - a review: its function, regulation in the testis, and role in male fertility
    Dolores D Mruk
    Population Council, Center for Biomedical Research, New York, NY, USA
    Contraception 65:305-11. 2002
    ..These results, taken collectively, suggest SOD(EX) is an important antioxidant molecule in the testis that is under germ cell regulation...
  90. doi Regulation of spermiogenesis, spermiation and blood-testis barrier dynamics: novel insights from studies on Eps8 and Arp3
    C Yan Cheng
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Biochem J 435:553-62. 2011
    ....
  91. doi A local autocrine axis in the testes that regulates spermatogenesis
    C Yan Cheng
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Nat Rev Endocrinol 6:380-95. 2010
    ..This local regulatory axis is the emerging target for male contraception...
  92. pmc An intracellular trafficking pathway in the seminiferous epithelium regulating spermatogenesis: a biochemical and molecular perspective
    C Yan Cheng
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, New York 10065, USA
    Crit Rev Biochem Mol Biol 44:245-63. 2009
    ..Herein, we provide a critical review based on the latest findings in the field regarding the regulation of these cellular events. These recent findings also open up a new window for investigators studying blood-tissue barriers...
  93. pmc Tight junctions in the testis: new perspectives
    Dolores D Mruk
    Population Council, Center for Biomedical Research, 1230 York Avenue, New York, NY 10065, USA
    Philos Trans R Soc Lond B Biol Sci 365:1621-35. 2010
    ..We also discuss how this information may apply to future studies investigating the movement of preleptotene spermatocytes across the BTB...
  94. pmc Emerging role for drug transporters at the blood-testis barrier
    Dolores D Mruk
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Trends Pharmacol Sci 32:99-106. 2011
    ..Modulation of efflux pump function via specific inhibitors could help to deliver contraceptives to the testis in the future...
  95. doi New perspectives in non-hormonal male contraception
    Dolores D Mruk
    Population Council, Center for Biomedical Research, 1230 York Avenue, New York, NY 10065, USA
    Trends Endocrinol Metab 19:57-64. 2008
    ..Through committed research, advocacy and support, male contraceptives are likely to become a valuable addition to the current choices of family planning...
  96. doi New frontiers in nonhormonal male contraception
    C Yan Cheng
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Contraception 82:476-82. 2010
    ..In this review, we discuss several approaches for fertility control involving the testis that one day may lead to the development of a nonhormonal male contraceptive...
  97. ncbi Cell-cell interactions at the ectoplasmic specialization in the testis
    Dolores D Mruk
    Population Council, Center for Biomedical Research, 1230 York Avenue, New York, New York 10021, USA
    Trends Endocrinol Metab 15:439-47. 2004
    ..This approach will not only provide a better understanding of ES dynamics, but also will yield innovative approaches for the development of male contraceptives...
  98. ncbi Adherens junction dynamics in the testis and spermatogenesis
    Wing Yee Lui
    Population Council, Center for Biomedical Research, New York, NY 10021, USA
    J Androl 24:1-14. 2003
  99. ncbi The Sertoli-spermatid junctional complex adhesion strength is affected in vitro by adjudin
    Katja M Wolski
    Department of Pathology and Cell Biology, University of South Florida College of Medicine, Tampa, FL 33612 4799, USA
    J Androl 27:790-4. 2006
    ..05). These data confirm that Adjudin is effective in reducing the strength of the STJC, identifying Adjudin as a potential contraceptive agent in the male by inducing spermatid sloughing and therefore oligospermia...
  100. pmc Disruption of Mtmr2 produces CMT4B1-like neuropathy with myelin outfolding and impaired spermatogenesis
    Alessandra Bolino
    Dulbecco Telethon Institute, San Raffaele Scientific Institute, 20132 Milan, Italy
    J Cell Biol 167:711-21. 2004
    ..We propose that Schwann cell-autonomous loss of Mtmr2-Dlg1 interaction dysregulates membrane homeostasis in the paranodal region, thereby producing outfolding and recurrent loops of myelin...