Luis J Montaner

Summary

Affiliation: The Wistar Institute
Country: USA

Publications

  1. pmc Constitutive gene expression in monocytes from chronic HIV-1 infection overlaps with acute Toll-like receptor induced monocyte activation profiles
    Bethsebah Gekonge
    The Wistar Institute, Philadelphia, Pennsylvania, United States of America
    PLoS ONE 7:e41153. 2012
  2. pmc Randomized trial of time-limited interruptions of protease inhibitor-based antiretroviral therapy (ART) vs. continuous therapy for HIV-1 infection
    Cynthia Firnhaber
    Clinical HIV Research Unit, Faculty of Health Sciences, Department of Medicine, University of the Witwatersrand, Johannesburg, South Africa
    PLoS ONE 6:e21450. 2011
  3. pmc Prioritizing CD4 count monitoring in response to ART in resource-constrained settings: a retrospective application of prediction-based classification
    Livio Azzoni
    Wistar Institute, Philadelphia, Pennsylvania, United States of America
    PLoS Med 9:e1001207. 2012
  4. ncbi request reprint HIV-1-specific CD4+ T cell responses in chronically HIV-1 infected blippers on antiretroviral therapy in relation to viral replication following treatment interruption
    Emmanouil Papasavvas
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    J Clin Immunol 26:40-54. 2006
  5. ncbi request reprint Advances in macrophage and dendritic cell biology in HIV-1 infection stress key understudied areas in infection, pathogenesis, and analysis of viral reservoirs
    Luis J Montaner
    The Wistar Institute, Philadelphia, PA 19104 4268, USA
    J Leukoc Biol 80:961-4. 2006
  6. pmc HIV type 1 viremia on ART is positively associated with polyclonal T cell proliferation in subjects with T cell IFN-gamma secretion levels comparable to those of uninfected subjects
    Emmanouil Papasavvas
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    AIDS Res Hum Retroviruses 24:1203-8. 2008
  7. pmc Delayed loss of control of plasma lipopolysaccharide levels after therapy interruption in chronically HIV-1-infected patients
    Emmanouil Papasavvas
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    AIDS 23:369-75. 2009
  8. ncbi request reprint Baseline viral load and immune activation determine the extent of reconstitution of innate immune effectors in HIV-1-infected subjects undergoing antiretroviral treatment
    Jihed Chehimi
    HIV Immunopathogenesis Laboratory, The Wistar Institute, Philadelphia, PA 19104, USA
    J Immunol 179:2642-50. 2007
  9. ncbi request reprint IL-13 acutely augments HIV-specific and recall responses from HIV-1-infected subjects in vitro by modulating monocytes
    Emmanouil Papasavvas
    The Wistar Institute, Philadelphia, PA 19104, USA
    J Immunol 175:5532-40. 2005
  10. ncbi request reprint Early and delayed benefits of HIV-1 suppression: timeline of recovery of innate immunity effector cells
    Livio Azzoni
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    AIDS 21:293-305. 2007

Detail Information

Publications78

  1. pmc Constitutive gene expression in monocytes from chronic HIV-1 infection overlaps with acute Toll-like receptor induced monocyte activation profiles
    Bethsebah Gekonge
    The Wistar Institute, Philadelphia, Pennsylvania, United States of America
    PLoS ONE 7:e41153. 2012
    ..In summary, our data support the presence of a sustained TLR-like gene activation profile in circulating monocyte from steady-state viremia in HIV-1 infected subjects...
  2. pmc Randomized trial of time-limited interruptions of protease inhibitor-based antiretroviral therapy (ART) vs. continuous therapy for HIV-1 infection
    Cynthia Firnhaber
    Clinical HIV Research Unit, Faculty of Health Sciences, Department of Medicine, University of the Witwatersrand, Johannesburg, South Africa
    PLoS ONE 6:e21450. 2011
    ..The clinical outcomes of short interruptions of PI-based ART regimens remains undefined...
  3. pmc Prioritizing CD4 count monitoring in response to ART in resource-constrained settings: a retrospective application of prediction-based classification
    Livio Azzoni
    Wistar Institute, Philadelphia, Pennsylvania, United States of America
    PLoS Med 9:e1001207. 2012
    ..Thus, methods to prioritize patients for CD4 count testing could improve treatment monitoring by optimizing resource allocation...
  4. ncbi request reprint HIV-1-specific CD4+ T cell responses in chronically HIV-1 infected blippers on antiretroviral therapy in relation to viral replication following treatment interruption
    Emmanouil Papasavvas
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    J Clin Immunol 26:40-54. 2006
    ..04) with no difference between groups by week 24. These data support that blippers have higher anti-HIV lymphoproliferative responses while on ART but experience equal to higher viral rebound as compared to matched non-blippers upon TI...
  5. ncbi request reprint Advances in macrophage and dendritic cell biology in HIV-1 infection stress key understudied areas in infection, pathogenesis, and analysis of viral reservoirs
    Luis J Montaner
    The Wistar Institute, Philadelphia, PA 19104 4268, USA
    J Leukoc Biol 80:961-4. 2006
    ....
  6. pmc HIV type 1 viremia on ART is positively associated with polyclonal T cell proliferation in subjects with T cell IFN-gamma secretion levels comparable to those of uninfected subjects
    Emmanouil Papasavvas
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    AIDS Res Hum Retroviruses 24:1203-8. 2008
    ..This finding suggests that T cell hyperresponsiveness may play a role in the pathogenesis of immune comorbidities on ART...
  7. pmc Delayed loss of control of plasma lipopolysaccharide levels after therapy interruption in chronically HIV-1-infected patients
    Emmanouil Papasavvas
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    AIDS 23:369-75. 2009
    ..Increased circulating levels of lipopolysaccharide (LPS) have been demonstrated in HIV-1-infected progressors. We investigated the effect of antiretroviral therapy (ART) interruptions on plasma LPS levels...
  8. ncbi request reprint Baseline viral load and immune activation determine the extent of reconstitution of innate immune effectors in HIV-1-infected subjects undergoing antiretroviral treatment
    Jihed Chehimi
    HIV Immunopathogenesis Laboratory, The Wistar Institute, Philadelphia, PA 19104, USA
    J Immunol 179:2642-50. 2007
    ....
  9. ncbi request reprint IL-13 acutely augments HIV-specific and recall responses from HIV-1-infected subjects in vitro by modulating monocytes
    Emmanouil Papasavvas
    The Wistar Institute, Philadelphia, PA 19104, USA
    J Immunol 175:5532-40. 2005
    ..Our data show that IL-13 is able to acutely enhance the function of the CD14(+) cell subset toward supporting Ag-specific cell-mediated responses in chronic HIV-1 infection...
  10. ncbi request reprint Early and delayed benefits of HIV-1 suppression: timeline of recovery of innate immunity effector cells
    Livio Azzoni
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    AIDS 21:293-305. 2007
    ..The kinetics of recovery for innate immune effectors following antiretroviral therapy are unknown...
  11. pmc Increased metallothionein gene expression, zinc, and zinc-dependent resistance to apoptosis in circulating monocytes during HIV viremia
    Andrea D Raymond
    The Wistar Institute, 3601 Spruce St, Philadelphia, PA 19104 4268, USA
    J Leukoc Biol 88:589-96. 2010
    ..Taken together, increases in MT gene expression and intracellular zinc levels may contribute directly to maintenance of an immune-activated monocyte by mediating an increased resistance to apoptosis during active HIV-1 viremia...
  12. pmc Increased soluble vascular cell adhesion molecule-1 plasma levels and soluble intercellular adhesion molecule-1 during antiretroviral therapy interruption and retention of elevated soluble vascular cellular adhesion molecule-1 levels following resumption
    Emmanouil Papasavvas
    The Wistar Institute, Philadelphia, PA 19104, USA
    AIDS 22:1153-61. 2008
    ....
  13. pmc Randomized, controlled trial of therapy interruption in chronic HIV-1 infection
    Emmanouil Papasavvas
    The Wistar Institute, Philadelphia, Pennsylvania, USA
    PLoS Med 1:e64. 2004
    ....
  14. ncbi request reprint Presence of human immunodeficiency virus-1-specific CD4 and CD8 cellular immune responses in children with full or partial virus suppression
    Emmanouil Papasavvas
    The Wistar Institute, Philadelphia, USA
    J Infect Dis 188:873-82. 2003
    ..Overall, a history of stable CD4(+) T cell counts, as a result of therapy that imparted full or partial virus suppression, was associated with increased levels of anti-HIV CD4(+) T helper responses and decreased T cell activation...
  15. pmc Inability of plasmacytoid dendritic cells to directly lyse HIV-infected autologous CD4+ T cells despite induction of tumor necrosis factor-related apoptosis-inducing ligand
    Jihed Chehimi
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    J Virol 84:2762-73. 2010
    ..Taken together, our data show a lack of sustained high levels of soluble IFN-alpha in chronic HIV-1 infection in vivo and document a lack of direct PDC cytolytic activity against autologous infected or uninfected CD4(+) T cells...
  16. pmc Circulating monocytes in HIV-1-infected viremic subjects exhibit an antiapoptosis gene signature and virus- and host-mediated apoptosis resistance
    Malavika S Giri
    The Wistar Institute, Philadelphia, PA 19104, USA
    J Immunol 182:4459-70. 2009
    ....
  17. pmc Antiretroviral therapy interruptions result in loss of protective humoral immunity to neoantigens in HIV-infected individuals
    Livio Azzoni
    The Wistar Institute, Philadelphia, Pennsylvania, USA
    AIDS 26:1355-62. 2012
    ..As ART interruption occur frequently in resource-constrained settings, we studied their effects on the ability to mount humoral immune responses against a neoantigen...
  18. pmc Metabolic and anthropometric parameters contribute to ART-mediated CD4+ T cell recovery in HIV-1-infected individuals: an observational study
    Livio Azzoni
    HIV 1 Immunopathogenesis Laboratory, The Wistar Institute, Philadelphia, PA, USA
    J Int AIDS Soc 14:37. 2011
    ..We sought to determine if adiposity and molecules associated with lipid metabolism may affect the response to ART and the degree of subsequent immune reconstitution, and to assess their ability to predict CD4 recovery...
  19. ncbi request reprint Synthetic imidazoquinolines potently and broadly activate the cellular immune response of patients with cutaneous T-cell lymphoma: synergy with interferon-gamma enhances production of interleukin-12
    Maria Wysocka
    Department of Dermatology, University of Pennsylvania, PA 19104, USA
    Clin Lymphoma Myeloma 7:524-34. 2007
    ....
  20. ncbi request reprint DR5 activation of caspase-8 induces DC maturation and immune enhancement in vivo
    Michael A Chattergoon
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Mol Ther 16:419-26. 2008
    ....
  21. pmc Natural killer cell activation distinguishes Mycobacterium tuberculosis-mediated immune reconstitution syndrome from chronic HIV and HIV/MTB coinfection
    Francesca Conradie
    Clinical HIV Research Unit, Department of Medicine, University of the Witwatersrand, Johannesburg, South Africa
    J Acquir Immune Defic Syndr 58:309-18. 2011
    ..We studied a cross-sectional cohort of HIV-infected subjects in Johannesburg (South Africa) to help define the immune correlates that best distinguish IRIS from ongoing MTB cases...
  22. pmc Association between HIV replication and serum leptin levels: an observational study of a cohort of HIV-1-infected South African women
    Livio Azzoni
    The Wistar Institute, Philadelphia, PA, USA
    J Int AIDS Soc 13:33. 2010
    ..Advanced HIV infection can result in lipoatrophy and wasting, even in the absence of ongoing opportunistic infections, suggesting that HIV may directly affect adipose tissue amount and distribution...
  23. pmc Retinoblastoma protein induction by HIV viremia or CCR5 in monocytes exposed to HIV-1 mediates protection from activation-induced apoptosis: ex vivo and in vitro study
    Bethsebah Gekonge
    Wistar Institute, Philadelphia, Pennsylvania, USA
    J Leukoc Biol 92:397-405. 2012
    ....
  24. ncbi request reprint NK cell lysis of HIV-1-infected autologous CD4 primary T cells: requirement for IFN-mediated NK activation by plasmacytoid dendritic cells
    Costin Tomescu
    HIV Immunopathogenesis Laboratory, Wistar Institute, Philadelphia, PA 19104, USA
    J Immunol 179:2097-104. 2007
    ..Overall, our data suggest that HIV-infected cells are not innately resistant to NK lysis, and that exogenous NK stimulation derived from PDC can trigger NK cytotoxicity against HIV-1-infected autologous CD4(+) primary T cells...
  25. ncbi request reprint Co-immunization with plasmid IL-12 generates a strong T-cell memory response in mice
    Michael A Chattergoon
    Department of Pathology, University of Pennsylvania School of Medicine, 422 Curie Blvd, 505 Stellar Chance Laboratories, Philadelphia, PA 19104, USA
    Vaccine 22:1744-50. 2004
    ..The strength of the immune response as well as the number of Ag-specific T-cells is proportional to the number of Ag-specific cells primed by the vaccination regimen...
  26. ncbi request reprint Sustained impairment of IFN-gamma secretion in suppressed HIV-infected patients despite mature NK cell recovery: evidence for a defective reconstitution of innate immunity
    Livio Azzoni
    Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA
    J Immunol 168:5764-70. 2002
    ....
  27. ncbi request reprint HIV-1 transmission and cytokine-induced expression of DC-SIGN in human monocyte-derived macrophages
    Jihed Chehimi
    HIV Immunopathogenesis Laboratory, The Wistar Institute, Philadelphia, PA, USA
    J Leukoc Biol 74:757-63. 2003
    ..39-48%), supporting the role of mannose-binding receptors in viral transmission. Taken together, results show that DC-SIGN regulation in MDM does not singly predict the transmission potential of this cell type...
  28. pmc Impact of protective killer inhibitory receptor/human leukocyte antigen genotypes on natural killer cell and T-cell function in HIV-1-infected controllers
    Costin Tomescu
    HIV Immunopathogenesis Laboratory, The Wistar Institute, Philadelphia, PA 19104, USA
    AIDS 26:1869-78. 2012
    ..However, a comparative analysis of the genotype and function of the innate and adaptive immune compartments in HIV-1-infected controller individuals has been understudied to date...
  29. pmc Increased microbial translocation in ≤ 180 days old perinatally human immunodeficiency virus-positive infants as compared with human immunodeficiency virus-exposed uninfected infants of similar age
    Emmanouil Papasavvas
    The Wistar Institute, Philadelphia, PA, USA
    Pediatr Infect Dis J 30:877-82. 2011
    ....
  30. pmc Pegylated Interferon alfa-2a monotherapy results in suppression of HIV type 1 replication and decreased cell-associated HIV DNA integration
    Livio Azzoni
    1HIV 1 Immunopathogenesis Laboratory, The Wistar Institute, Philadelphia, PA, USA
    J Infect Dis 207:213-22. 2013
    ..Antiretroviral therapy (ART)-mediated immune reconstitution fails to restore the capacity of the immune system to spontaneously control human immunodeficiency virus (HIV) replication...
  31. ncbi request reprint Concurrent measures of total and integrated HIV DNA monitor reservoirs and ongoing replication in eradication trials
    Angela M Mexas
    Division of Transfusion Medicine, Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, 19104, USA
    AIDS 26:2295-306. 2012
    ..Therefore, we aimed to develop strategies to sensitively measure changes in these parameters in patients on and off antiretroviral therapy (ART). Achieving these goals may help evaluate the effects of future clinical trials...
  32. ncbi request reprint HIV and cells of macrophage/dendritic lineage and other non-T cell reservoirs: new answers yield new questions
    Ronald G Collman
    Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
    J Leukoc Biol 74:631-4. 2003
    ..This issue of JLB contains several reviews and original reports resulting from the workshop that address recent progress and highlight the current research questions regarding these cell types...
  33. ncbi request reprint Lack of persistent drug-resistant mutations evaluated within and between treatment interruptions in chronically HIV-1-infected patients
    Emmanouil Papasavvas
    Wistar Institute and the Philadelphia Field Initiating Group for HIV 1 Trials, Philadelphia, Pennsylvania, USA
    AIDS 17:2337-43. 2003
    ..To determine the effect of treatment interruptions (TI) on the evolution and persistence of drug-resistant viruses in chronically HIV-1-infected suppressed patients...
  34. ncbi request reprint Microarray data on gene modulation by HIV-1 in immune cells: 2000-2006
    Malavika S Giri
    HIV Immunopathogenesis Laboratory, Wistar Institute, 3601 Spruce St, Room 480, Philadelphia, PA 19104, USA
    J Leukoc Biol 80:1031-43. 2006
    ....
  35. ncbi request reprint Persistent decreases in blood plasmacytoid dendritic cell number and function despite effective highly active antiretroviral therapy and increased blood myeloid dendritic cells in HIV-infected individuals
    Jihed Chehimi
    HIV Immunopathogenesis Laboratory, Wistar Institute, Philadelphia, PA 19104, USA
    J Immunol 168:4796-801. 2002
    ..The persistent impairment of certain DC subsets may result in a sustained defect in DC-mediated innate immune functions despite an effective treatment regimen...
  36. pmc Increased CD34+/KDR+ cells are not associated with carotid artery intima-media thickness progression in chronic HIV-positive subjects
    Emmanouil Papasavvas
    The Wistar Institute, Philadelphia, PA, USA
    Antivir Ther 17:557-63. 2012
    ..We hypothesized that circulating EPCs would be inversely associated with carotid artery intima-media thickness (c-IMT) changes in HIV-infected subjects...
  37. ncbi request reprint Enhancement of the host immune responses in cutaneous T-cell lymphoma by CpG oligodeoxynucleotides and IL-15
    Maria Wysocka
    Department of Dermatology, University of Pennsylvania, 245 CRB, 415 Curie Blvd, Philadelphia, PA 19104, USA
    Blood 104:4142-9. 2004
    ..These findings have important implications for the potential enhancement of antitumor immunity among patients with advanced CTCL...
  38. pmc Identification of a 251 gene expression signature that can accurately detect M. tuberculosis in patients with and without HIV co-infection
    Noor Dawany
    Center for Systems and Computational Biology, The Wistar Institute, Philadelphia, Pennsylvania, United States of America
    PLoS ONE 9:e89925. 2014
    ..Here we report a gene signature that can identify a tuberculosis infection in patients co-infected with HIV as well as in the absence of HIV...
  39. pmc Antiviral effects of autologous CD4 T cells genetically modified with a conditionally replicating lentiviral vector expressing long antisense to HIV
    Pablo Tebas
    Department of Medicine and Center for AIDS Research, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA
    Blood 121:1524-33. 2013
    ..We conclude that gene-modified T cells have the potential to decrease the fitness of HIV-1 and conditionally replicative lentiviral vectors have a promising safety profile in T cells...
  40. ncbi request reprint Lessons learned from HIV treatment interruption: safety, correlates of immune control, and drug sparing
    Livio Azzoni
    HIV Immunopathogenesis Laboratory, The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Curr HIV Res 1:329-42. 2003
    ..Based on ongoing research, we identify the topics to be targeted in future studies...
  41. ncbi request reprint Flt3 ligand treatment reverses endotoxin tolerance-related immunoparalysis
    Maria Wysocka
    Department of Dermatology, University of Pennsylvania, Philadelphia, 19104, USA
    J Immunol 174:7398-402. 2005
    ..Notably, administration of flt3 ligand rapidly reverses immunoparalysis in vivo, accelerating and amplifying repopulation of tissues with proinflammatory and immunoregulatory cytokine-producing dendritic cells...
  42. pmc Increased plasmacytoid dendritic cell maturation and natural killer cell activation in HIV-1 exposed, uninfected intravenous drug users
    Costin Tomescu
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    AIDS 24:2151-60. 2010
    ..However, the exact mechanism contributing to NK activation in HIV-1 exposed, uninfected intravenous drug users (EU-IDU) remains to be elucidated...
  43. pmc Retention of viability, cytotoxicity, and response to IL-2, IL-15, or IFN-alpha by human NK cells after CD107a degranulation
    Costin Tomescu
    HIV Immunopathogenesis Laboratory, Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    J Leukoc Biol 85:871-6. 2009
    ..Overall, our data argue for the strength of the NK cell compartment in the continuous surveillance of tumor and virally infected cells in the body and highlight the use of using CD107a expression as a stable marker for NK cytotoxicity...
  44. pmc Diverse and high prevalence of human papillomavirus associated with a significant high rate of cervical dysplasia in human immunodeficiency virus-infected women in Johannesburg, South Africa
    Cynthia Firnhaber
    Department of Medicine, University of Witwatersrand, Johannesburg, South Africa
    Acta Cytol 53:10-7. 2009
    ..To evaluate the epidemiology of the human papillomavirus (HPV) type and correlate it with the Papanicolaou smears in human immunodeficiency virus-seropositive women in Johannesburg, South Africa...
  45. ncbi request reprint Immune reconstitution
    Drew Weissman
    Division of Infectious Diseases, University of Pennsylvania, 522B Johnson Pavilion, Philadelphia, PA 19104, USA
    Clin Lab Med 22:719-40. 2002
    ....
  46. ncbi request reprint Costimulatory molecule immune enhancement in a plasmid vaccine model is regulated in part through the Ig constant-like domain of CD80/86
    Michael G Agadjanyan
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, School of Medicine, Philadelphia, PA 19104, USA
    J Immunol 171:4311-9. 2003
    ....
  47. ncbi request reprint Complex proteome prefractionation using microscale solution isoelectrofocusing
    Hsin Yao Tang
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Expert Rev Proteomics 2:295-306. 2005
    ..This review focuses on the application of microscale solution isoelectrofocusing prefractionation and subsequent downstream strategies in expanding protein profiling capacities and mining low-abundance proteins of complex proteomes...
  48. pmc A fused alpha-beta "mini-spectrin" mimics the intact erythrocyte spectrin head-to-head tetramer
    Sandra L Harper
    Center for Systems and Computational Biology, The Wistar Institute, 3601 Spruce St, Philadelphia, PA 19104, USA
    J Biol Chem 285:11003-12. 2010
    ..Caffrey, M. S., Johnson, M. E., and Fung, L. W. (2003) J. Biol. Chem. 278, 21837-21844). This fusion protein should serve as a useful template for structural and functional studies of the divalent tetramer site...
  49. pmc Activation and repression of interleukin-12 p40 transcription by erythroid Kruppel-like factor in macrophages
    Qi Luo
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 279:18451-6. 2004
    ..The bifunctional control of IL-12 p40 by EKLF and its modulation of NFkappaB support a potential function for this factor in orchestrating IL-12 p40 production in macrophages...
  50. pmc Serial cervicovaginal exposures with replication-deficient SIVsm induce higher dendritic cell (pDC) and CD4+ T-cell infiltrates not associated with prevention but a more severe SIVmac251 infection of rhesus macaques
    Shaheed A Abdulhaqq
    Department of Immunology, The Wistar Institute, Philadelphia, PA Caribbean Primate Research Center and Animal Resources Center, University of Puerto Rico UPR, San Juan, PR University of Minnesota Medical School, Minneapolis, MN Division of Biostatistics and Epidemiology, University of Massachusetts, Amherst, MA Tulane National Primate Research Center, Covington, LA Departments of Microbiology and Internal Medicine, UPR Medical School, San Juan, PR and School of Biological Sciences and Nebraska Center for Virology, University of Nebraska, Lincoln, NE
    J Acquir Immune Defic Syndr 65:405-13. 2014
    ..We tested the impact of repeated cervicovaginal exposures to noninfectious, defective SIV particles over 72 hours on a subsequent cervicovaginal challenge with replication competent SIV...
  51. pmc Effects of behavioral stress reduction Transcendental Meditation intervention in persons with HIV
    Sumedha Chhatre
    a Department of Psychiatry, HIV AIDS Prevention Research Division, University of Pennsylvania, Philadelphia, PA, USA
    AIDS Care 25:1291-7. 2013
    ..TM stress reduction intervention in community dwelling adults with HIV is viable and can enhance health-related quality of life. Further research with large sample and longer follow-up is needed to validate our results...
  52. ncbi request reprint A structural model of the erythrocyte spectrin heterodimer initiation site determined using homology modeling and chemical cross-linking
    Donghai Li
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    J Biol Chem 283:1553-62. 2008
    ..This model supports the hypothesis that initial docking of the correct alpha and beta repeats from among many very similar repeats in both subunits is driven primarily by long range electrostatic interactions...
  53. ncbi request reprint Depletion of multiple high-abundance proteins improves protein profiling capacities of human serum and plasma
    Lynn A Echan
    The Wistar Institute, Philadelphia, PA 19104, USA
    Proteomics 5:3292-303. 2005
    ..Hence, non2-D methods such as protein array pixelation are more promising strategies for detecting lower abundance proteins after depleting the six abundant proteins...
  54. ncbi request reprint Microscale solution isoelectrofocusing: a sample prefractionation method for comprehensive proteome analysis
    Xun Zuo
    The Wistar Institute, Philadelphia, PA, USA
    Methods Mol Biol 244:361-75. 2004
  55. pmc Forced unfolding of proteins within cells
    Colin P Johnson
    Biophysical Engineering Lab, University of Pennsylvania, Philadelphia, PA 19104, USA
    Science 317:663-6. 2007
    ....
  56. ncbi request reprint Influence of lateral association on forced unfolding of antiparallel spectrin heterodimers
    Richard Law
    Department of Chemical and Biomolecular Engineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6315, USA
    J Biol Chem 279:16410-6. 2004
    ..Weak lateral interactions do not inhibit unfolding, but strong lateral interactions facilitate simultaneous unfolding analogous to serial repeat coupling within spectrin family proteins...
  57. ncbi request reprint Comprehensive analysis of complex proteomes using microscale solution isoelectrofocusing prior to narrow pH range two-dimensional electrophoresis
    Xun Zuo
    The Wistar Institute, Philadelphia, PA 19104, USA
    Proteomics 2:58-68. 2002
    ....
  58. ncbi request reprint In vivo phosphorylation of human erythrocyte spectrin occurs in a sequential manner
    Hsin Yao Tang
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Biochemistry 43:4251-62. 2004
    ....
  59. ncbi request reprint Initiation and propagation of spectrin heterodimer assembly involves distinct energetic processes
    Donghai Li
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Biochemistry 46:10585-94. 2007
    ..On the basis of this analysis of heterodimer thermodynamics, a detailed model of spectrin dimer assembly is proposed...
  60. ncbi request reprint A novel four-dimensional strategy combining protein and peptide separation methods enables detection of low-abundance proteins in human plasma and serum proteomes
    Hsin Yao Tang
    The Wistar Institute, Philadelphia, PA 19104, USA
    Proteomics 5:3329-42. 2005
    ..With further refinement, this method has the potential for even higher capacity and higher throughput...
  61. ncbi request reprint Enhanced analysis of human breast cancer proteomes using micro-scale solution isoelectrofocusing combined with high resolution 1-D and 2-D gels
    Xun Zuo
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 782:253-65. 2002
    ....
  62. doi request reprint Protein profiling by microscale solution isoelectrofocusing (MicroSol-IEF)
    Xun Zuo
    The Wistar Institute, Philadelphia, Pennsylvania, USA
    Curr Protoc Protein Sci . 2005
    ..In addition, methods are provided for evaluating the effectiveness of the prefractionation, using 1-D and 2-D gel electrophoresis. Approaches for quantitative protein profiling that incorporate MicroSol-IEF are also discussed...
  63. pmc Pathway shifts and thermal softening in temperature-coupled forced unfolding of spectrin domains
    Richard Law
    Biophysical Engineering Lab, Institute for Medicine and Engineering, and School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6315, USA
    Biophys J 85:3286-93. 2003
    ..In sum, structural changes with temperature correlate with both single-molecule unfolding forces and shifts in unfolding pathways...
  64. pmc Comparison of extensive protein fractionation and repetitive LC-MS/MS analyses on depth of analysis for complex proteomes
    Huan Wang
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    J Proteome Res 9:1032-40. 2010
    ..Hence, high reproducibility of complex proteomes, such as human cancer cell lysates, readily can be achieved when using multidimensional separation methods with good depth of analysis...
  65. doi request reprint Overview of proteome analysis
    Nadeem Ali-Khan
    The Wistar Institute, Philadelphia, Pennsylvania, USA
    Curr Protoc Protein Sci . 2003
    ..The complexity of different types of proteomes, the merits of targeted versus global proteome studies, and the advantages of alternative separation and analysis technologies are discussed...
  66. doi request reprint Human serum and plasma proteomics
    Ian Musselman
    The Wistar Institute, Philadelphia, Pennsylvania, USA
    Curr Protoc Protein Sci . 2005
    ..Additional protein profiling methods and strategies are also discussed...
  67. pmc Structural and functional effects of hereditary hemolytic anemia-associated point mutations in the alpha spectrin tetramer site
    Massimiliano Gaetani
    The Wistar Institute, Philadelphia, PA 19104, USA
    Blood 111:5712-20. 2008
    ..All alpha0 HE/HPP mutations studied here appear to exert their destabilizing effects through molecular recognition rather than structural mechanisms...
  68. doi request reprint Microscale isoelectric focusing in solution: a method for comprehensive and quantitative proteome analysis using 1-D and 2-D DIGE combined with MicroSol IEF prefractionation
    Mee Jung Han
    The Wistar Institute, Philadelphia, Pennsylvania, USA
    Methods Mol Biol 424:241-56. 2008
    ..The combination of DIGE and MicroSol IEF produces a powerful method for more comprehensive and quantitative comparison of protein profiles of complex proteomes...
  69. pmc A comprehensive model of the spectrin divalent tetramer binding region deduced using homology modeling and chemical cross-linking of a mini-spectrin
    Donghai Li
    Center for Systems and Computational Biology, The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 285:29535-45. 2010
    ....
  70. pmc Structural basis for dimerization in DNA recognition by Gal4
    Manqing Hong
    The Wistar Institute, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA
    Structure 16:1019-26. 2008
    ..Associated biochemical studies show that the dimerization domain of Gal4 is important for DNA binding and protein thermostability. We also map the interaction surface of the Gal4 dimerization domain with Gal11P...
  71. pmc Pathogenic proline mutation in the linker between spectrin repeats: disease caused by spectrin unfolding
    Colin P Johnson
    Molecular and Cell Biophysics Laboratory, University of Pennsylvania, 3699 Market Street, Philadelphia, PA 19104, USA
    Blood 109:3538-43. 2007
    ..The results suggest a molecular mechanism for the high frequency of disease caused by proline mutations in spectrin linkers...
  72. ncbi request reprint Higher dimensional (Hi-D) separation strategies dramatically improve the potential for cancer biomarker detection in serum and plasma
    Seth A Hoffman
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 849:43-52. 2007
    ..In contrast, new higher dimensional (Hi-D) separation strategies, utilizing more than two dimensions of fractionation, can profile the low abundance proteome...
  73. pmc The proteome of the mouse photoreceptor sensory cilium complex
    Qin Liu
    F M Kirby Center for Molecular Ophthalmology, Scheie Eye Institute, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Mol Cell Proteomics 6:1299-317. 2007
    ....
  74. pmc Mass spectrometric and computational analysis of cytokine-induced alterations in the astrocyte secretome
    Sarah Dunn Keene
    Stokes Research Institute and Department of Pediatrics, Children s Hospital of Philadelphia, Philadelphia, PA 19104 4318, USA
    Proteomics 9:768-82. 2009
    ....
  75. doi request reprint Determination of disulfide-bond linkages in proteins
    Hsin Yao Tang
    The Wistar Institute, Philadelphia, Pennsylvania, USA
    Curr Protoc Protein Sci . 2004
    ..A partial reduction and alkylation strategy for mapping disulfide linkages in peptides with multiple disulfide bonds is also presented...
  76. ncbi request reprint Disulfide bond assignments of secreted Frizzled-related protein-1 provide insights about Frizzled homology and netrin modules
    Jae Min Chong
    Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 277:5134-44. 2002
    ..The major C-terminal processing event was removal of the terminal amino acid (Lys(313)) with only a trace amount of unprocessed protein detected...
  77. pmc Cdc13 N-terminal dimerization, DNA binding, and telomere length regulation
    Meghan T Mitchell
    The Wistar Institute, 3601 Spruce St, Philadelphia, PA 19104, USA
    Mol Cell Biol 30:5325-34. 2010
    ..The multiple DNA binding domains and dimeric nature of Cdc13 offer unique insights into how it coordinates the recruitment and regulation of telomerase access to the telomeres...
  78. ncbi request reprint Constitutive and induced expression of DC-SIGN on dendritic cell and macrophage subpopulations in situ and in vitro
    Elizabeth J Soilleux
    Department of Molecular Histopathology, University of Cambridge, United Kingdom
    J Leukoc Biol 71:445-57. 2002
    ....

Research Grants36

  1. IMMUNOBIOLOGY OF INTERLUKIN-12
    Luis Montaner; Fiscal Year: 2003
    ..Analyze the mechanisms of acute induction and priming for cytokine production induced by IL-12 and costimulation. ..
  2. Pediatric Immune Correlates of Early Anti-HIV Therapy
    Luis Montaner; Fiscal Year: 2009
    ....
  3. Pediatric Immune Correlates of Early Anti-HIV Therapy
    Luis Montaner; Fiscal Year: 2005
    ....
  4. FLOW CORE UPGRADE--CYTOMATION MOFLO CYTOFLUORIMETER
    Luis Montaner; Fiscal Year: 2001
    ....
  5. Cellular innate activation as a tactic to prevent HIV-1 transmission
    LUIS J contact MONTANER; Fiscal Year: 2010
    ..abstract_text> ..
  6. HIV Therapy & Interruption RCT in Resource Poor Clinic
    Luis Montaner; Fiscal Year: 2004
    ..abstract_text> ..
  7. Pediatric Immune Correlates of Early Anti-HIV Therapy
    Luis Montaner; Fiscal Year: 2006
    ....
  8. HIV Therapy & Interruption RCT in Resource Poor Clinic
    Luis Montaner; Fiscal Year: 2007
    ..abstract_text> ..
  9. Pediatric Immune Correlates of Early Anti-HIV Therapy
    Luis Montaner; Fiscal Year: 2007
    ....
  10. Antiviral activity of Peg-IFN alpha in chronic HIV-1
    Luis Montaner; Fiscal Year: 2007
    ..and The Gladstone Institute of Virology and Immunology. ..
  11. NK/DC Cross-Talk and Antiviral Response
    Luis Montaner; Fiscal Year: 2009
    ..As the rate of response to therapy varies from about 60% in mono-infection to <25% in HIV/HCV co-infected individuals, it is imperative that added research be conducted in understanding clearance mechanisms. ..
  12. NK Cell Activation and Function in HIV-1 Exposed Uninfected IV Drug Users
    David W Speicher; Fiscal Year: 2010
    ....
  13. APCs and Innate Immunity in HIV Disease
    Luis Montaner; Fiscal Year: 2004
    ..a hypothesis-driven collaborative effort by The Wistar Institute, the Infectious Disease Division of the University of Pennsylvania Hospital, Philadelphia FIGHT, Schering-Plough and the Multiple AIDS Cohort Study ..
  14. HIV Therapy & Interruption RCT in Resource Poor Clinic
    Luis Montaner; Fiscal Year: 2003
    ..abstract_text> ..
  15. IMMUNOBIOLOGY OF INTERLUKIN-12
    Luis Montaner; Fiscal Year: 2000
    ..Analyze the mechanisms of acute induction and priming for cytokine production induced by IL-12 and costimulation. ..
  16. IMMUNE AND VIRAL OUTCOMES OF HIV-1 THERAPY INTERRUPTION
    Luis Montaner; Fiscal Year: 2000
    ....
  17. IMMUNOBIOLOGY OF INTERLUKIN-12
    Luis Montaner; Fiscal Year: 2002
    ..Analyze the mechanisms of acute induction and priming for cytokine production induced by IL-12 and costimulation. ..
  18. IMMUNE AND VIRAL OUTCOMES OF HIV-1 THERAPY INTERRUPTION
    Luis Montaner; Fiscal Year: 2002
    ....
  19. APCs and Innate Immunity in HIV Disease
    Luis Montaner; Fiscal Year: 2002
    ..a hypothesis-driven collaborative effort by The Wistar Institute, the Infectious Disease Division of the University of Pennsylvania Hospital, Philadelphia FIGHT, Schering-Plough and the Multiple AIDS Cohort Study ..
  20. IMMUNE AND VIRAL OUTCOMES OF HIV-1 THERAPY INTERRUPTION
    Luis Montaner; Fiscal Year: 2003
    ....
  21. NK/DC Cross-Talk and Antiviral Response
    Luis J Montaner; Fiscal Year: 2010
    ..As the rate of response to therapy varies from about 60% in mono-infection to <25% in HIV/HCV co-infected individuals, it is imperative that added research be conducted in understanding clearance mechanisms. ..
  22. Cellular innate activation as a tactic to prevent HIV-1 transmission
    Luis Montaner; Fiscal Year: 2009
    ..abstract_text> ..
  23. IMMUNOBIOLOGY OF INTERLUKIN-12
    Luis Montaner; Fiscal Year: 2001
    ..Analyze the mechanisms of acute induction and priming for cytokine production induced by IL-12 and costimulation. ..
  24. APCs and Innate Immunity in HIV Disease
    Luis Montaner; Fiscal Year: 2005
    ..a hypothesis-driven collaborative effort by The Wistar Institute, the Infectious Disease Division of the University of Pennsylvania Hospital, Philadelphia FIGHT, Schering-Plough and the Multiple AIDS Cohort Study ..
  25. IMMUNE AND VIRAL OUTCOMES OF HIV-1 THERAPY INTERRUPTION
    Luis Montaner; Fiscal Year: 2001
    ....
  26. APCs and Innate Immunity in HIV Disease
    Luis Montaner; Fiscal Year: 2003
    ..a hypothesis-driven collaborative effort by The Wistar Institute, the Infectious Disease Division of the University of Pennsylvania Hospital, Philadelphia FIGHT, Schering-Plough and the Multiple AIDS Cohort Study ..
  27. Pediatric Immune Correlates of Early Anti-HIV Therapy
    Luis J Montaner; Fiscal Year: 2010
    ....