Norman H Lee
Affiliation: The Institute for Genomic Research
- Assessing unmodified 70-mer oligonucleotide probe performance on glass-slide microarraysHong-Ying Wang
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
Genome Biol 4:R5. 2003..CONCLUSIONS: Microarrays of UV cross-linked unmodified oligonucleotides provided sensitive and specific measurements for most of the genes studied...
- Gene expression profiling of lymphoblastoid cell lines from monozygotic twins discordant in severity of autism reveals differential regulation of neurologically relevant genesValerie W Hu
The George Washington University Medical Center, Dept of Biochemistry and Molecular Biology, 2300 Eye St, N W Washington, DC 20037, USA
BMC Genomics 7:118. 2006....
- A robust method for the amplification of RNA in the sense orientationNicholas F Marko
The George Washington University Medical Center, USA
BMC Genomics 6:27. 2005..Alternatives lack extensive validation and are often confounded by problems with bias or yield attributable to their greater biological and technical complexity...
- Alanine scanning mutagenesis of conserved arginine/lysine-arginine/lysine-X-X-arginine/lysine G protein-activating motifs on m1 muscarinic acetylcholine receptorsN H Lee
Institute for Genomic Research, Department of Molecular and Cellular Biology, Rockville, Maryland 20850, USA
Mol Pharmacol 50:140-8. 1996..In contrast, all three residues must be present for stimulation of PI hydrolysis by a partial agonist...
- Nerve growth factor regulation of m4 muscarinic receptor mRNA stability but not gene transcription requires mitogen-activated protein kinase activityN H Lee
Department of Molecular and Cellular Biology, The Institute for Genomic Research, Rockville, Maryland 20850, USA
J Biol Chem 273:22317-25. 1998..Finally, transcription of the m4 gene can be induced by all three growth factors but is not dependent on MAPK activity, unlike growth factor-induced m4 mRNA stabilization...
- Genomic approaches for reconstructing gene networksNorman H Lee
The Institute for Genomic Research, Department of Functional Genomics, Rockville, MD 20850, USA
Pharmacogenomics 6:245-58. 2005..In this review, several different genomic technologies are discussed, and example studies that are promoting new discoveries and hypotheses are detailed...
- Within the fold: assessing differential expression measures and reproducibility in microarray assaysIvana V Yang
The Institute for Genomic Research, Rockville, MD 20850, USA
Genome Biol 3:research0062. 2002..We show that pooled reference samples should be based not only on the expression of individual genes in each cell line but also on the expression levels of genes within cell lines...
- Physiogenomic resources for rat models of heart, lung and blood disordersRenae L Malek
TREX, The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
Nat Genet 38:234-9. 2006..We performed a variety of data-mining manipulations on the profiles and used complementary physiological data from the PhysGen resource to demonstrate how TREX can be used by the cardiovascular community for hypothesis generation...
- Combined application of behavior genetics and microarray analysis to identify regional expression themes and gene-behavior associationsNoah E Letwin
Department of Functional Genomics, The Institute for Genomic Research, Rockville, Maryland 20850, USA
J Neurosci 26:5277-87. 2006..The approaches and data set described herein serve as a valuable resource to investigate the genetic underpinning of complex behaviors...
- Molecular cloning, tissue-specific expression, and chromosomal localization of a novel nerve growth factor-regulated G-protein- coupled receptor, nrg-1M Glickman
Department of Molecular Biology, The Institute of Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
Mol Cell Neurosci 14:141-52. 1999..Chromosomal mapping employing a rat somatic cell readiation hybrid panel demonstrated that nrg-1 is linked to marker D8Rat54 and tightly associated with H218 on chromosome 8...
- Microarrays: an overviewNorman H Lee
Department of Functional Genomics, Institute for Genomic Research, Rockville, MD, USA
Methods Mol Biol 353:265-300. 2007..In this chapter, the underlying principles regarding microarrays are reviewed, to serve as a guide when navigating through this powerful technology...
- Identification of Src transformation fingerprint in human colon cancerRenae L Malek
Department of Functional Genomics, The Institute for Genomic Research, 9712 Medical Center Dr, Rockville, Maryland, MD 20850, USA
Oncogene 21:7256-65. 2002..Supportive Supplemental Data can be viewed at http://pga.tigr.org/PGApubs.shtml...
- Comparative expressed-sequence-tag analysis of differential gene expression profiles in PC-12 cells before and after nerve growth factor treatmentN H Lee
Institute for Genomic Research, Gaithersburg, MD 20878, USA
Proc Natl Acad Sci U S A 92:8303-7. 1995....
- Genome sequence of Aedes aegypti, a major arbovirus vectorVishvanath Nene
Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
Science 316:1718-23. 2007..An increase in genes encoding odorant binding, cytochrome P450, and cuticle domains relative to An. gambiae suggests that members of these protein families underpin some of the biological differences between the two mosquito species...
- Nrg-1 belongs to the endothelial differentiation gene family of G protein-coupled sphingosine-1-phosphate receptorsR L Malek
Department of Molecular and Cellular Biology, Institute for Genomic Research, Rockville, Maryland 20850, USA
J Biol Chem 276:5692-9. 2001..The antiproliferative effect, like S1P-mediated ERK1/2 inhibition, was orthovanadate-sensitive and pertussis toxin-insensitive. Our results indicate that EDG-8, a member of the EDG-1 subfamily, couples to unique signaling pathways...
- Transcriptional profiling with a blood pressure QTL interval-specific oligonucleotide arrayBina Joe
Department of Physiology and Cardiovascular Genomics, Medical University of Ohio, Toledo, Ohio 43614 5804, USA
Physiol Genomics 23:318-26. 2005..These results demonstrate the importance of using a saturated oligonucleotide array for identifying and prioritizing differentially expressed positional candidate genes of a BP QTL...
- Growth hormone-mediated alteration of fuel metabolism in the aged rat as determined from transcript profilesPetra Tollet-Egnell
Department of Molecular Medicine, Karolinska Institutet, Karolinska Hospital, 171 76 Stockholm, Sweden
Physiol Genomics 16:261-7. 2004..Serum levels of triglycerides, high-density lipoprotein (HDL) cholesterol, and total cholesterol were higher in the old animals than in the young and normalized by GH treatment...
- Identification of H-Ras, RhoA, Rac1 and Cdc42 responsive genesHidemi Teramoto
Oral and Pharyngeal Cancer Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892-4330, USA
Oncogene 22:2689-97. 2003..64..
- Iterative microarray and RNA interference-based interrogation of the SRC-induced invasive phenotypeRosalyn B Irby
Department of Surgery, H. Lee Moffit Cancer Center and Research Institute, College of Medicine, University of South Florida, Tampa, Florida 33612, USA
Cancer Res 65:1814-21. 2005..In this manner, numerous members of a "transcriptional cascade" pathway for metastatic activity have been identified and functionally validated...
- Autocrine activation of an osteopontin-CD44-Rac pathway enhances invasion and transformation by H-RasV12Hidemi Teramoto
Oral and Pharyngeal Cancer Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892 4330, USA
Oncogene 24:489-501. 2005..Indeed, four genes were selected for knockdown, each resulting in a disruption of foci formation and/or invasion. These results underscore the role of aberrant autocrine signaling and transcriptional networking during tumorigenesis...
- Molecular networks in Dahl salt-sensitive hypertension based on transcriptome analysis of a panel of consomic ratsMingyu Liang
Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
Physiol Genomics 34:54-64. 2008....
- Schistosoma mansoni genome project: an updatePhilip T LoVerde
Department of Microbiology and Immunology, State University of New York, Buffalo, NY 14214, USA
Parasitol Int 53:183-92. 2004..Functional genomics is expected to accelerate the development of control and treatment strategies for schistosomiasis...
- Proteomic analysis of laser-captured paraffin-embedded tissues: a molecular portrait of head and neck cancer progressionVyomesh Patel
Oral and Pharyngeal Cancer Branch, National Institute of Craniofacial and Dental Research, NIH, Bethesda, Maryland 20892, USA
Clin Cancer Res 14:1002-14. 2008..A proteomic analysis of HNSCC lesions may help identify novel molecular targets for the early detection, prevention, and treatment of HNSCC...
- Associating quantitative behavioral traits with gene expression in the brain: searching for diamonds in the hayAnat Reiner Benaim
Department of Statistics and Operation Research, Tel Aviv University
Bioinformatics 23:2239-46. 2007..The hierarchical FDR testing strategies presented here can serve beyond the structure of our motivating example study to any complex microarray study. Supplementary information: Supplementary data are available at Bioinformatics online...
- Identification of Pax5 as a target of MTA1 in B-cell lymphomasSeetharaman Balasenthil
Department of Molecular and Cellular Oncology, The University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
Cancer Res 67:7132-8. 2007..Significance of these murine studies was established by evidence showing a widespread up-regulation of both MTA1 and Pax5 in DLBCL from humans. These observations provide in vivo genetic evidence for a role of MTA1 in lymphomagenesis...
- Physiogenomic strategies and resources to associate genes with rat models of heart, lung and blood disordersNorman H Lee
Department of Pharmacology and Physiology, The George Washington University Medical Center, 2300 Eye Street NW, Washington, DC 20037, USA
Exp Physiol 92:992-1002. 2007..Also discussed are ways to harness two recent publicly available resources that have been created to investigate the role of genes and environment on cardiovascular physiology and pathophysiology...
- Cross-talk of expression quantitative trait loci within 2 interacting blood pressure quantitative trait lociNorman H Lee
Department of Pharmacology and Physiology, George Washington University, Washington, DC, USA
Hypertension 50:1126-33. 2007..A network of transcription factors potentially affecting blood pressure could be traced, lending support to our hypothesis...
- Identification of Myc-mediated death response pathways by microarray analysisQiang Yu
Advanced Technology Center, Center for Cancer Research, NCI National Institutes of Health, Gaithersburg, MD 20877, USA
J Biol Chem 277:13059-66. 2002..Thus, we have proposed that removal of Myc-mediated repression of apoptotic signals, combined with Myc-associated acceleration of the p53 responsive pathway, results in complete and rapid cell death following DNA damage...
- Patterns of liver gene expression governed by TRbetaAmilcar Flores-Morales
Department of Molecular Medicine, Karolinska Institute, Stockholm 17176, Sweden
Mol Endocrinol 16:1257-68. 2002....
- cDNA microarray profiling of rat mammary gland carcinomas induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine and 7,12-dimethylbenz[a]anthraceneLiang Shan
Chemical Carcinogenesis Section, Laboratory of Experimental Carcinogenesis, National Cancer Institute, NIH, Bethesda, MD 20892, USA
Carcinogenesis 23:1561-8. 2002..The similarities in gene expression between PhIP- and DMBA-induced carcinomas raise the possibility that several molecular pathways for rat mammary gland transformation are maintained irrespective of the carcinogenic initiating agent...
- Divergent responses of chondrocytes and endothelial cells to shear stress: cross-talk among COX-2, the phase 2 response, and apoptosisZachary R Healy
Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA
Proc Natl Acad Sci U S A 102:14010-5. 2005....
- The Schistosoma mansoni gene index: gene discovery and biology by reconstruction and analysis of expressed gene sequencesJoseph M Merrick
Department of Microbiology, State University of New York, Buffalo, New York 14214, USA
J Parasitol 89:261-9. 2003..The utility of EST analysis is demonstrated by identifying new protease genes, which may be involved in hemoglobin degradation...
- Differential transactivation of sphingosine-1-phosphate receptors modulates NGF-induced neurite extensionRachelle E Toman
Department of Biochemistry, Virginia Commonwealth University School of Medicine, VCU Medical Center, Richmond 23298, USA
J Cell Biol 166:381-92. 2004..Thus, differential transactivation of S1P receptors by NGF regulates antagonistic signaling pathways that modulate neurite extension...
- Molecular profiling of LGL leukemia reveals role of sphingolipid signaling in survival of cytotoxic lymphocytesMithun Vinod Shah
Penn State Hershey Cancer Institute, Milton S Hershey Medical College, Hershey, PA, USA
Blood 112:770-81. 2008..Collectively, these results show a role for sphingolipid-mediated signaling as a mechanism for long-term survival of CTLs. Therapeutic targeting of this pathway, such as use of FTY720, may have efficacy in LGL leukemia...