Norman H Lee

Summary

Affiliation: The Institute for Genomic Research
Country: USA

Publications

  1. pmc Assessing unmodified 70-mer oligonucleotide probe performance on glass-slide microarrays
    Hong Ying Wang
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Genome Biol 4:R5. 2003
  2. pmc Gene expression profiling of lymphoblastoid cell lines from monozygotic twins discordant in severity of autism reveals differential regulation of neurologically relevant genes
    Valerie W Hu
    The George Washington University Medical Center, Dept of Biochemistry and Molecular Biology, 2300 Eye St, N W Washington, DC 20037, USA
    BMC Genomics 7:118. 2006
  3. pmc A robust method for the amplification of RNA in the sense orientation
    Nicholas F Marko
    The George Washington University Medical Center, USA
    BMC Genomics 6:27. 2005
  4. ncbi Alanine scanning mutagenesis of conserved arginine/lysine-arginine/lysine-X-X-arginine/lysine G protein-activating motifs on m1 muscarinic acetylcholine receptors
    N H Lee
    Institute for Genomic Research, Department of Molecular and Cellular Biology, Rockville, Maryland 20850, USA
    Mol Pharmacol 50:140-8. 1996
  5. ncbi Nerve growth factor regulation of m4 muscarinic receptor mRNA stability but not gene transcription requires mitogen-activated protein kinase activity
    N H Lee
    Department of Molecular and Cellular Biology, The Institute for Genomic Research, Rockville, Maryland 20850, USA
    J Biol Chem 273:22317-25. 1998
  6. ncbi Genomic approaches for reconstructing gene networks
    Norman H Lee
    The Institute for Genomic Research, Department of Functional Genomics, Rockville, MD 20850, USA
    Pharmacogenomics 6:245-58. 2005
  7. ncbi Physiogenomic resources for rat models of heart, lung and blood disorders
    Renae L Malek
    TREX, The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
    Nat Genet 38:234-9. 2006
  8. ncbi Microarrays: an overview
    Norman H Lee
    Department of Functional Genomics, Institute for Genomic Research, Rockville, MD, USA
    Methods Mol Biol 353:265-300. 2007
  9. ncbi Molecular cloning, tissue-specific expression, and chromosomal localization of a novel nerve growth factor-regulated G-protein- coupled receptor, nrg-1
    M Glickman
    Department of Molecular Biology, The Institute of Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Mol Cell Neurosci 14:141-52. 1999
  10. pmc Within the fold: assessing differential expression measures and reproducibility in microarray assays
    Ivana V Yang
    The Institute for Genomic Research, Rockville, MD 20850, USA
    Genome Biol 3:research0062. 2002

Research Grants

  1. RAT GENE EXPRESSION ANATOMY
    Norman Lee; Fiscal Year: 2004
  2. Mapping Gene Networks in Colon Cancer
    Norman Lee; Fiscal Year: 2007

Detail Information

Publications34

  1. pmc Assessing unmodified 70-mer oligonucleotide probe performance on glass-slide microarrays
    Hong Ying Wang
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Genome Biol 4:R5. 2003
    ..The feasibility of using unmodified oligonucleotides to accurately measure changes in gene expression is also unclear...
  2. pmc Gene expression profiling of lymphoblastoid cell lines from monozygotic twins discordant in severity of autism reveals differential regulation of neurologically relevant genes
    Valerie W Hu
    The George Washington University Medical Center, Dept of Biochemistry and Molecular Biology, 2300 Eye St, N W Washington, DC 20037, USA
    BMC Genomics 7:118. 2006
    ....
  3. pmc A robust method for the amplification of RNA in the sense orientation
    Nicholas F Marko
    The George Washington University Medical Center, USA
    BMC Genomics 6:27. 2005
    ..Alternatives lack extensive validation and are often confounded by problems with bias or yield attributable to their greater biological and technical complexity...
  4. ncbi Alanine scanning mutagenesis of conserved arginine/lysine-arginine/lysine-X-X-arginine/lysine G protein-activating motifs on m1 muscarinic acetylcholine receptors
    N H Lee
    Institute for Genomic Research, Department of Molecular and Cellular Biology, Rockville, Maryland 20850, USA
    Mol Pharmacol 50:140-8. 1996
    ..In contrast, all three residues must be present for stimulation of PI hydrolysis by a partial agonist...
  5. ncbi Nerve growth factor regulation of m4 muscarinic receptor mRNA stability but not gene transcription requires mitogen-activated protein kinase activity
    N H Lee
    Department of Molecular and Cellular Biology, The Institute for Genomic Research, Rockville, Maryland 20850, USA
    J Biol Chem 273:22317-25. 1998
    ..Finally, transcription of the m4 gene can be induced by all three growth factors but is not dependent on MAPK activity, unlike growth factor-induced m4 mRNA stabilization...
  6. ncbi Genomic approaches for reconstructing gene networks
    Norman H Lee
    The Institute for Genomic Research, Department of Functional Genomics, Rockville, MD 20850, USA
    Pharmacogenomics 6:245-58. 2005
    ..In this review, several different genomic technologies are discussed, and example studies that are promoting new discoveries and hypotheses are detailed...
  7. ncbi Physiogenomic resources for rat models of heart, lung and blood disorders
    Renae L Malek
    TREX, The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
    Nat Genet 38:234-9. 2006
    ..We performed a variety of data-mining manipulations on the profiles and used complementary physiological data from the PhysGen resource to demonstrate how TREX can be used by the cardiovascular community for hypothesis generation...
  8. ncbi Microarrays: an overview
    Norman H Lee
    Department of Functional Genomics, Institute for Genomic Research, Rockville, MD, USA
    Methods Mol Biol 353:265-300. 2007
    ..In this chapter, the underlying principles regarding microarrays are reviewed, to serve as a guide when navigating through this powerful technology...
  9. ncbi Molecular cloning, tissue-specific expression, and chromosomal localization of a novel nerve growth factor-regulated G-protein- coupled receptor, nrg-1
    M Glickman
    Department of Molecular Biology, The Institute of Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Mol Cell Neurosci 14:141-52. 1999
    ..Chromosomal mapping employing a rat somatic cell readiation hybrid panel demonstrated that nrg-1 is linked to marker D8Rat54 and tightly associated with H218 on chromosome 8...
  10. pmc Within the fold: assessing differential expression measures and reproducibility in microarray assays
    Ivana V Yang
    The Institute for Genomic Research, Rockville, MD 20850, USA
    Genome Biol 3:research0062. 2002
    ..Further, the analysis of a large number of expression profiles is facilitated by a common reference sample, the construction of which must be carefully addressed...
  11. ncbi Combined application of behavior genetics and microarray analysis to identify regional expression themes and gene-behavior associations
    Noah E Letwin
    Department of Functional Genomics, The Institute for Genomic Research, Rockville, Maryland 20850, USA
    J Neurosci 26:5277-87. 2006
    ..The approaches and data set described herein serve as a valuable resource to investigate the genetic underpinning of complex behaviors...
  12. ncbi Identification of Src transformation fingerprint in human colon cancer
    Renae L Malek
    Department of Functional Genomics, The Institute for Genomic Research, 9712 Medical Center Dr, Rockville, Maryland, MD 20850, USA
    Oncogene 21:7256-65. 2002
    ..Supportive Supplemental Data can be viewed at http://pga.tigr.org/PGApubs.shtml...
  13. pmc Comparative expressed-sequence-tag analysis of differential gene expression profiles in PC-12 cells before and after nerve growth factor treatment
    N H Lee
    Institute for Genomic Research, Gaithersburg, MD 20878, USA
    Proc Natl Acad Sci U S A 92:8303-7. 1995
    ....
  14. pmc Genome sequence of Aedes aegypti, a major arbovirus vector
    Vishvanath Nene
    Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Science 316:1718-23. 2007
    ..An increase in genes encoding odorant binding, cytochrome P450, and cuticle domains relative to An. gambiae suggests that members of these protein families underpin some of the biological differences between the two mosquito species...
  15. ncbi Nrg-1 belongs to the endothelial differentiation gene family of G protein-coupled sphingosine-1-phosphate receptors
    R L Malek
    Department of Molecular and Cellular Biology, Institute for Genomic Research, Rockville, Maryland 20850, USA
    J Biol Chem 276:5692-9. 2001
    ..The antiproliferative effect, like S1P-mediated ERK1/2 inhibition, was orthovanadate-sensitive and pertussis toxin-insensitive. Our results indicate that EDG-8, a member of the EDG-1 subfamily, couples to unique signaling pathways...
  16. ncbi Transcriptional profiling with a blood pressure QTL interval-specific oligonucleotide array
    Bina Joe
    Department of Physiology and Cardiovascular Genomics, Medical University of Ohio, Toledo, Ohio 43614 5804, USA
    Physiol Genomics 23:318-26. 2005
    ..These results demonstrate the importance of using a saturated oligonucleotide array for identifying and prioritizing differentially expressed positional candidate genes of a BP QTL...
  17. ncbi Growth hormone-mediated alteration of fuel metabolism in the aged rat as determined from transcript profiles
    Petra Tollet-Egnell
    Department of Molecular Medicine, Karolinska Institutet, Karolinska Hospital, 171 76 Stockholm, Sweden
    Physiol Genomics 16:261-7. 2004
    ..Serum levels of triglycerides, high-density lipoprotein (HDL) cholesterol, and total cholesterol were higher in the old animals than in the young and normalized by GH treatment...
  18. ncbi Identification of H-Ras, RhoA, Rac1 and Cdc42 responsive genes
    Hidemi Teramoto
    Oral and Pharyngeal Cancer Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892 4330, USA
    Oncogene 22:2689-97. 2003
    ..64..
  19. ncbi Iterative microarray and RNA interference-based interrogation of the SRC-induced invasive phenotype
    Rosalyn B Irby
    Department of Surgery, H Lee Moffit Cancer Center and Research Institute, College of Medicine, University of South Florida, Tampa, Florida 33612, USA
    Cancer Res 65:1814-21. 2005
    ..In this manner, numerous members of a "transcriptional cascade" pathway for metastatic activity have been identified and functionally validated...
  20. ncbi Autocrine activation of an osteopontin-CD44-Rac pathway enhances invasion and transformation by H-RasV12
    Hidemi Teramoto
    Oral and Pharyngeal Cancer Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892 4330, USA
    Oncogene 24:489-501. 2005
    ..Indeed, four genes were selected for knockdown, each resulting in a disruption of foci formation and/or invasion. These results underscore the role of aberrant autocrine signaling and transcriptional networking during tumorigenesis...
  21. ncbi Molecular networks in Dahl salt-sensitive hypertension based on transcriptome analysis of a panel of consomic rats
    Mingyu Liang
    Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Physiol Genomics 34:54-64. 2008
    ....
  22. ncbi Schistosoma mansoni genome project: an update
    Philip T LoVerde
    Department of Microbiology and Immunology, State University of New York, Buffalo, NY 14214, USA
    Parasitol Int 53:183-92. 2004
    ..Functional genomics is expected to accelerate the development of control and treatment strategies for schistosomiasis...
  23. doi Proteomic analysis of laser-captured paraffin-embedded tissues: a molecular portrait of head and neck cancer progression
    Vyomesh Patel
    Oral and Pharyngeal Cancer Branch, National Institute of Craniofacial and Dental Research, NIH, Bethesda, Maryland 20892, USA
    Clin Cancer Res 14:1002-14. 2008
    ..A proteomic analysis of HNSCC lesions may help identify novel molecular targets for the early detection, prevention, and treatment of HNSCC...
  24. ncbi Associating quantitative behavioral traits with gene expression in the brain: searching for diamonds in the hay
    Anat Reiner-Benaim
    Department of Statistics and Operation Research, Tel Aviv University
    Bioinformatics 23:2239-46. 2007
    ..The hierarchical FDR testing strategies presented here can serve beyond the structure of our motivating example study to any complex microarray study. Supplementary information: Supplementary data are available at Bioinformatics online...
  25. ncbi Identification of Pax5 as a target of MTA1 in B-cell lymphomas
    Seetharaman Balasenthil
    Department of Molecular and Cellular Oncology, The University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Cancer Res 67:7132-8. 2007
    ..Significance of these murine studies was established by evidence showing a widespread up-regulation of both MTA1 and Pax5 in DLBCL from humans. These observations provide in vivo genetic evidence for a role of MTA1 in lymphomagenesis...
  26. ncbi Physiogenomic strategies and resources to associate genes with rat models of heart, lung and blood disorders
    Norman H Lee
    Department of Pharmacology and Physiology, The George Washington University Medical Center, 2300 Eye Street NW, Washington, DC 20037, USA
    Exp Physiol 92:992-1002. 2007
    ..Also discussed are ways to harness two recent publicly available resources that have been created to investigate the role of genes and environment on cardiovascular physiology and pathophysiology...
  27. ncbi Cross-talk of expression quantitative trait loci within 2 interacting blood pressure quantitative trait loci
    Norman H Lee
    Department of Pharmacology and Physiology, George Washington University, Washington, DC, USA
    Hypertension 50:1126-33. 2007
    ..A network of transcription factors potentially affecting blood pressure could be traced, lending support to our hypothesis...
  28. ncbi Identification of Myc-mediated death response pathways by microarray analysis
    Qiang Yu
    Advanced Technology Center, Center for Cancer Research, NCI National Institutes of Health, Gaithersburg, MD 20877, USA
    J Biol Chem 277:13059-66. 2002
    ..Thus, we have proposed that removal of Myc-mediated repression of apoptotic signals, combined with Myc-associated acceleration of the p53 responsive pathway, results in complete and rapid cell death following DNA damage...
  29. ncbi Patterns of liver gene expression governed by TRbeta
    Amilcar Flores-Morales
    Department of Molecular Medicine, Karolinska Institute, Stockholm 17176, Sweden
    Mol Endocrinol 16:1257-68. 2002
    ....
  30. ncbi cDNA microarray profiling of rat mammary gland carcinomas induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine and 7,12-dimethylbenz[a]anthracene
    Liang Shan
    Chemical Carcinogenesis Section, Laboratory of Experimental Carcinogenesis, National Cancer Institute, NIH, Bethesda, MD 20892, USA
    Carcinogenesis 23:1561-8. 2002
    ..The similarities in gene expression between PhIP- and DMBA-induced carcinomas raise the possibility that several molecular pathways for rat mammary gland transformation are maintained irrespective of the carcinogenic initiating agent...
  31. pmc Divergent responses of chondrocytes and endothelial cells to shear stress: cross-talk among COX-2, the phase 2 response, and apoptosis
    Zachary R Healy
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA
    Proc Natl Acad Sci U S A 102:14010-5. 2005
    ....
  32. ncbi The Schistosoma mansoni gene index: gene discovery and biology by reconstruction and analysis of expressed gene sequences
    Joseph M Merrick
    Department of Microbiology, State University of New York, Buffalo, New York 14214, USA
    J Parasitol 89:261-9. 2003
    ..The utility of EST analysis is demonstrated by identifying new protease genes, which may be involved in hemoglobin degradation...
  33. pmc Differential transactivation of sphingosine-1-phosphate receptors modulates NGF-induced neurite extension
    Rachelle E Toman
    Department of Biochemistry, Virginia Commonwealth University School of Medicine, VCU Medical Center, Richmond 23298, USA
    J Cell Biol 166:381-92. 2004
    ..Thus, differential transactivation of S1P receptors by NGF regulates antagonistic signaling pathways that modulate neurite extension...
  34. pmc Molecular profiling of LGL leukemia reveals role of sphingolipid signaling in survival of cytotoxic lymphocytes
    Mithun Vinod Shah
    Penn State Hershey Cancer Institute, Milton S Hershey Medical College, Hershey, PA, USA
    Blood 112:770-81. 2008
    ..Collectively, these results show a role for sphingolipid-mediated signaling as a mechanism for long-term survival of CTLs. Therapeutic targeting of this pathway, such as use of FTY720, may have efficacy in LGL leukemia...

Research Grants2

  1. RAT GENE EXPRESSION ANATOMY
    Norman Lee; Fiscal Year: 2004
    ..abstract_text> ..
  2. Mapping Gene Networks in Colon Cancer
    Norman Lee; Fiscal Year: 2007
    ....