Edward L LeCluyse

Summary

Affiliation: The Hamner Institutes for Health Sciences
Country: USA

Publications

  1. pmc Organotypic liver culture models: meeting current challenges in toxicity testing
    Edward L LeCluyse
    The Institute for Chemical Safety Sciences, The Hamner Institutes for Health Sciences, Research Triangle Park, NC, USA
    Crit Rev Toxicol 42:501-48. 2012
  2. doi request reprint Xenobiotic-metabolizing enzyme and transporter gene expression in primary cultures of human hepatocytes modulated by ToxCast chemicals
    Daniel M Rotroff
    US Environmental Protection Agency, Office of Research and Development, National Center for Computational Toxicology, Research Triangle Park, North Carolina, USA
    J Toxicol Environ Health B Crit Rev 13:329-46. 2010
  3. ncbi request reprint Liver biomarker and in vitro assessment confirm the hepatic origin of aminotransferase elevations lacking histopathological correlate in beagle dogs treated with GABAA receptor antagonist NP260
    Alison H Harrill
    College of Public Health, The University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA The Hamner University of North Carolina Institute for Drug Safety Sciences, The Hamner Institutes for Health Sciences, Research Triangle Park, NC 27709, USA Electronic address
    Toxicol Appl Pharmacol 277:131-7. 2014
  4. doi request reprint Incorporating human dosimetry and exposure into high-throughput in vitro toxicity screening
    Daniel M Rotroff
    National Center for Computational Toxicology, Office of Research and Development, United States Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Toxicol Sci 117:348-58. 2010
  5. ncbi request reprint Human CYP2C8 is transcriptionally regulated by the nuclear receptors constitutive androstane receptor, pregnane X receptor, glucocorticoid receptor, and hepatic nuclear factor 4alpha
    Stephen S Ferguson
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Mol Pharmacol 68:747-57. 2005
  6. doi request reprint Cross-species comparisons of transcriptomic alterations in human and rat primary hepatocytes exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin
    Michael B Black
    The Hamner Institutes for Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Toxicol Sci 127:199-215. 2012
  7. ncbi request reprint Isolation and culture of primary hepatocytes from resected human liver tissue
    Edward L LeCluyse
    CellzDirect Invitrogen Corp, Durham, NC, USA
    Methods Mol Biol 640:57-82. 2010
  8. ncbi request reprint A map of the PPARĪ± transcription regulatory network for primary human hepatocytes
    Patrick D McMullen
    The Hamner Institutes for Health Sciences, Institute for Chemical Safety Sciences, 6 Davis Drive, PO Box 12137, Research Triangle Park, NC 27709, USA
    Chem Biol Interact 209:14-24. 2014
  9. ncbi request reprint CYP3A induction by N-hydroxyformamide tumor necrosis factor-alpha converting enzyme/matrix metalloproteinase inhibitors use of a pregname X receptor activation assay and primary hepatocyte culture for assessing induction potential in humans
    Timothy K Tippin
    Drug Metabolism and Pharmacokinetics Department, Metabolic and Viral Diseases Center of Excellence in Drug Discovery, GlaxoSmithKline, 5 Moore Drive, Research Triangle Park, NC 27709, USA
    Drug Metab Dispos 31:870-7. 2003

Collaborators

Detail Information

Publications9

  1. pmc Organotypic liver culture models: meeting current challenges in toxicity testing
    Edward L LeCluyse
    The Institute for Chemical Safety Sciences, The Hamner Institutes for Health Sciences, Research Triangle Park, NC, USA
    Crit Rev Toxicol 42:501-48. 2012
    ..In the future, a balanced approach between sample throughput and biological relevance should provide better in vitro tools that are complementary with animal testing and assist in conducting more predictive human risk assessment...
  2. doi request reprint Xenobiotic-metabolizing enzyme and transporter gene expression in primary cultures of human hepatocytes modulated by ToxCast chemicals
    Daniel M Rotroff
    US Environmental Protection Agency, Office of Research and Development, National Center for Computational Toxicology, Research Triangle Park, North Carolina, USA
    J Toxicol Environ Health B Crit Rev 13:329-46. 2010
    ..Significant relative risk associations with rodent in vivo chronic toxicity effects are reported for the five major receptor pathways. These gene expression data are being incorporated into the larger ToxCast predictive modeling effort...
  3. ncbi request reprint Liver biomarker and in vitro assessment confirm the hepatic origin of aminotransferase elevations lacking histopathological correlate in beagle dogs treated with GABAA receptor antagonist NP260
    Alison H Harrill
    College of Public Health, The University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA The Hamner University of North Carolina Institute for Drug Safety Sciences, The Hamner Institutes for Health Sciences, Research Triangle Park, NC 27709, USA Electronic address
    Toxicol Appl Pharmacol 277:131-7. 2014
    ..Taken together, the data indicate that a diagnostic biomarker approach, coupled with sensitive in vitro screening strategies, may facilitate interpretation of toxicity potential when an adaptive event masks the underlying toxicity. ..
  4. doi request reprint Incorporating human dosimetry and exposure into high-throughput in vitro toxicity screening
    Daniel M Rotroff
    National Center for Computational Toxicology, Office of Research and Development, United States Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA
    Toxicol Sci 117:348-58. 2010
    ..Importantly, these tools are necessary to move beyond hazard rankings to estimates of possible in vivo responses based on in vitro screens...
  5. ncbi request reprint Human CYP2C8 is transcriptionally regulated by the nuclear receptors constitutive androstane receptor, pregnane X receptor, glucocorticoid receptor, and hepatic nuclear factor 4alpha
    Stephen S Ferguson
    Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Mol Pharmacol 68:747-57. 2005
    ..In summary, the present studies show that CAR, PXR, GR, and HNF4alpha can regulate CYP2C8 expression and identify specific cis-elements within the promoter that control these regulatory pathways...
  6. doi request reprint Cross-species comparisons of transcriptomic alterations in human and rat primary hepatocytes exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin
    Michael B Black
    The Hamner Institutes for Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Toxicol Sci 127:199-215. 2012
    ..The results provide evidence for divergent cross-species gene expression changes in response to TCDD and are consistent with epidemiological and clinical evidence showing humans to be less sensitive to TCDD-induced hepatotoxicity...
  7. ncbi request reprint Isolation and culture of primary hepatocytes from resected human liver tissue
    Edward L LeCluyse
    CellzDirect Invitrogen Corp, Durham, NC, USA
    Methods Mol Biol 640:57-82. 2010
    ....
  8. ncbi request reprint A map of the PPARĪ± transcription regulatory network for primary human hepatocytes
    Patrick D McMullen
    The Hamner Institutes for Health Sciences, Institute for Chemical Safety Sciences, 6 Davis Drive, PO Box 12137, Research Triangle Park, NC 27709, USA
    Chem Biol Interact 209:14-24. 2014
    ....
  9. ncbi request reprint CYP3A induction by N-hydroxyformamide tumor necrosis factor-alpha converting enzyme/matrix metalloproteinase inhibitors use of a pregname X receptor activation assay and primary hepatocyte culture for assessing induction potential in humans
    Timothy K Tippin
    Drug Metabolism and Pharmacokinetics Department, Metabolic and Viral Diseases Center of Excellence in Drug Discovery, GlaxoSmithKline, 5 Moore Drive, Research Triangle Park, NC 27709, USA
    Drug Metab Dispos 31:870-7. 2003
    ..In conclusion, GW3333 is a potential inducer of CYP3A expression in vivo in humans, but other N-hydroxyformamides are less likely to induce CYP3A...