Joseph Krzycki

Summary

Affiliation: The Ohio State University
Country: USA

Publications

  1. ncbi request reprint Pyrrolysine encoded by UAG in Archaea: charging of a UAG-decoding specialized tRNA
    Gayathri Srinivasan
    Department of Microbiology, Ohio State University, Columbus, OH 43210, USA
    Science 296:1459-62. 2002
  2. ncbi request reprint Function of genetically encoded pyrrolysine in corrinoid-dependent methylamine methyltransferases
    Joseph A Krzycki
    Department of Microbiology, OSU Biochemistry Program, Ohio State University, Columbus, Ohio 43210, USA
    Curr Opin Chem Biol 8:484-91. 2004
  3. doi request reprint The path of lysine to pyrrolysine
    Joseph A Krzycki
    Department of Microbiology, 484 West 12th Avenue, Columbus, OH 43210, United States The Ohio State University Biochemistry Program, The Ohio State University, 484 West 12th Avenue, Columbus, OH 43210, United States Electronic address
    Curr Opin Chem Biol 17:619-25. 2013
  4. ncbi request reprint The direct genetic encoding of pyrrolysine
    Joseph A Krzycki
    Department of Microbiology, OSU Biochemistry Program, Ohio State University, Columbus, OH 43210, USA
    Curr Opin Microbiol 8:706-12. 2005
  5. ncbi request reprint Direct charging of tRNA(CUA) with pyrrolysine in vitro and in vivo
    Sherry K Blight
    Department of Microbiology, 484 West 12th Avenue, The Ohio State University, Columbus, Ohio 43210, USA
    Nature 431:333-5. 2004
  6. ncbi request reprint Characterization of a Methanosarcina acetivorans mutant unable to translate UAG as pyrrolysine
    Anirban Mahapatra
    Department of Microbiology, Ohio State University, 484 West 12th Avenue, Columbus, OH 43210, USA
    Mol Microbiol 59:56-66. 2006
  7. ncbi request reprint The residue mass of L-pyrrolysine in three distinct methylamine methyltransferases
    Jitesh A Soares
    Department of Microbiology, Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 280:36962-9. 2005
  8. ncbi request reprint A new UAG-encoded residue in the structure of a methanogen methyltransferase
    Bing Hao
    Department of Biochemistry, The Ohio State University, 484 West 12th Avenue, Columbus, OH 43210, USA
    Science 296:1462-6. 2002
  9. ncbi request reprint Reactivity and chemical synthesis of L-pyrrolysine- the 22(nd) genetically encoded amino acid
    Bing Hao
    Department of Biochemistry, The Ohio State University, Columbus, OH 43210, USA
    Chem Biol 11:1317-24. 2004
  10. pmc A natural genetic code expansion cassette enables transmissible biosynthesis and genetic encoding of pyrrolysine
    David G Longstaff
    Department of Microbiology, Campus Chemical Instrument Center Mass Spectrometry and Proteomics Facility, Ohio State University, 484 West 12th Avenue, Columbus, OH 43210, USA
    Proc Natl Acad Sci U S A 104:1021-6. 2007

Research Grants

Collaborators

  • JAMES FERRY
  • Everly Conway de Macario
  • JOHN REEVE
  • R H White
  • JOHN LEIGH
  • Anirban Mahapatra
  • Jitesh A Soares
  • David G Longstaff
  • Gang Zhao
  • Patrick T Kang
  • Michael K Chan
  • Bing Hao
  • Ross C Larue
  • Kari B Green-Church
  • William W Metcalf
  • Gayathri Srinivasan
  • Wen Tai Li
  • Tsuneo Ferguson
  • Tanja Lienard
  • Gerhard Gottschalk
  • Jun Kai Zhang
  • Liwen Zhang
  • Sherry K Blight
  • Tsuneo K Ferguson
  • Carey M James
  • James E Galagan
  • Jonathan Bechtel
  • Kerstin B Richter
  • Andrew Meyer
  • Joseph E Faust
  • Michael Chan
  • Asmita Patel
  • Jon Amster
  • Jeremy J Wolff
  • R Benjamin Jones
  • Nanette M Kleinholz
  • Rhonda L Pitsch
  • Judith Gallucci
  • Edward Chang
  • Robert D Barber
  • Andrea Tirrell
  • Alice Roy
  • Jinfeng Liu
  • H Craig Kuettner
  • Eric Lander
  • Robin Johnson
  • Jerome Naylor
  • Matthew G Endrizzi
  • Weimin Gong
  • Pendexter Macdonald
  • Ronald V Swanson
  • Isaac Cann
  • Will FitzHugh
  • Kurt DeArellano
  • Matthew Pritchett
  • David E Graham
  • Steven H Zinder
  • Bruce Birren
  • Reiner Hedderich
  • Chad Nusbaum
  • Nicole Allen
  • Ken F Jarrell
  • Cheryl Ingram-Smith
  • Andrew Zimmer
  • Ian Paulsen
  • Sarah Calvo
  • Reinhard Engels
  • Lauren Linton
  • Paul McEwan
  • Kevin McKernan
  • Kerry Smith
  • Adam M Guss
  • Hua Jing
  • Kevin R Sowers
  • Alberto J L Macario
  • Serge Smirnov
  • Weixi Li
  • David A Grahame
  • Timothy A Springer
  • Deven Atnoor
  • Adam Brown
  • Lowell A Umayam
  • Jessica Talamas
  • Owen White
  • Biswarup Mukhopadhyay
  • Nicole Stange-Thomann
  • Wenjuan Ye

Detail Information

Publications14

  1. ncbi request reprint Pyrrolysine encoded by UAG in Archaea: charging of a UAG-decoding specialized tRNA
    Gayathri Srinivasan
    Department of Microbiology, Ohio State University, Columbus, OH 43210, USA
    Science 296:1459-62. 2002
    ..Charging a tRNA(CUA) with lysine is a likely first step in translating UAG amber codons as pyrrolysine in certain methanogens. Our results indicate that pyrrolysine is the 22nd genetically encoded natural amino acid...
  2. ncbi request reprint Function of genetically encoded pyrrolysine in corrinoid-dependent methylamine methyltransferases
    Joseph A Krzycki
    Department of Microbiology, OSU Biochemistry Program, Ohio State University, Columbus, Ohio 43210, USA
    Curr Opin Chem Biol 8:484-91. 2004
    ..This reaction is essential for methane formation from methylamines, and may underlie the retention of pyrrolysine in the genetic code of methanogens...
  3. doi request reprint The path of lysine to pyrrolysine
    Joseph A Krzycki
    Department of Microbiology, 484 West 12th Avenue, Columbus, OH 43210, United States The Ohio State University Biochemistry Program, The Ohio State University, 484 West 12th Avenue, Columbus, OH 43210, United States Electronic address
    Curr Opin Chem Biol 17:619-25. 2013
    ..Mechanisms have been proposed for both the mutase and the ligase, and structures now exist for each, setting the stage for a more detailed understanding of how pyrrolysine is synthesized and functions in bacteria and archaea. ..
  4. ncbi request reprint The direct genetic encoding of pyrrolysine
    Joseph A Krzycki
    Department of Microbiology, OSU Biochemistry Program, Ohio State University, Columbus, OH 43210, USA
    Curr Opin Microbiol 8:706-12. 2005
    ..Thus, the recruitment of pyrrolysine into the genetic code involved evolution of the first non-canonical aminoacyl-tRNA synthetase and cognate tRNA to be described from nature...
  5. ncbi request reprint Direct charging of tRNA(CUA) with pyrrolysine in vitro and in vivo
    Sherry K Blight
    Department of Microbiology, 484 West 12th Avenue, The Ohio State University, Columbus, Ohio 43210, USA
    Nature 431:333-5. 2004
    ..This is the first example from nature of direct aminoacylation of a tRNA with a non-canonical amino acid and shows that the genetic code of E. coli can be expanded to include UAG-directed pyrrolysine incorporation into proteins...
  6. ncbi request reprint Characterization of a Methanosarcina acetivorans mutant unable to translate UAG as pyrrolysine
    Anirban Mahapatra
    Department of Microbiology, Ohio State University, 484 West 12th Avenue, Columbus, OH 43210, USA
    Mol Microbiol 59:56-66. 2006
    ..These results indicate that in-depth genetic analysis of UAG translation as pyrrolysine is feasible, as deletion of pylT is conditionally lethal depending on growth substrate...
  7. ncbi request reprint The residue mass of L-pyrrolysine in three distinct methylamine methyltransferases
    Jitesh A Soares
    Department of Microbiology, Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 280:36962-9. 2005
    ....
  8. ncbi request reprint A new UAG-encoded residue in the structure of a methanogen methyltransferase
    Bing Hao
    Department of Biochemistry, The Ohio State University, 484 West 12th Avenue, Columbus, OH 43210, USA
    Science 296:1462-6. 2002
    ..Instead it appears consistent with a lysine in amide-linkage to (4R,5R)-4-substituted-pyrroline-5-carboxylate. We suggest that this amino acid be named l-pyrrolysine...
  9. ncbi request reprint Reactivity and chemical synthesis of L-pyrrolysine- the 22(nd) genetically encoded amino acid
    Bing Hao
    Department of Biochemistry, The Ohio State University, Columbus, OH 43210, USA
    Chem Biol 11:1317-24. 2004
    ..Future applications of this synthetic L-pyrrolysine include its in vivo incorporation into recombinant proteins...
  10. pmc A natural genetic code expansion cassette enables transmissible biosynthesis and genetic encoding of pyrrolysine
    David G Longstaff
    Department of Microbiology, Campus Chemical Instrument Center Mass Spectrometry and Proteomics Facility, Ohio State University, 484 West 12th Avenue, Columbus, OH 43210, USA
    Proc Natl Acad Sci U S A 104:1021-6. 2007
    ..Analogous cassettes may have served similar functions for other amino acids during the evolutionary expansion of the canonical genetic code...
  11. pmc RamA, a protein required for reductive activation of corrinoid-dependent methylamine methyltransferase reactions in methanogenic archaea
    Tsuneo Ferguson
    Department of Microbiology and The Ohio State Biochemistry Program, Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 284:2285-95. 2009
    ..These results suggest that RamA mediates reductive activation of corrinoid proteins and that it is the first functional archetype of COG3894, a family of redox proteins of unknown function...
  12. doi request reprint Specificity of pyrrolysyl-tRNA synthetase for pyrrolysine and pyrrolysine analogs
    Wen Tai Li
    Department of Chemistry, The Ohio State University, Columbus, OH 43210, USA
    J Mol Biol 385:1156-64. 2009
    ....
  13. ncbi request reprint Class I and class II lysyl-tRNA synthetase mutants and the genetic encoding of pyrrolysine in Methanosarcina spp
    Anirban Mahapatra
    Department of Microbiology, Ohio State University, 484 West 12th Avenue, Columbus, OH 43210, USA
    Mol Microbiol 64:1306-18. 2007
    ..However, decreased lysyl-tRNA(Lys) in the lysS mutant provides a possible rationale for stable transfer of the bacterial lysS gene to methanoarchaea...
  14. pmc The genome of M. acetivorans reveals extensive metabolic and physiological diversity
    James E Galagan
    Whitehead Institute Center for Genome Research, Cambridge, Massachusetts 02141, USA
    Genome Res 12:532-42. 2002
    ..acetivorans a powerful model organism for the study of archaeal biology. [Sequence, data, annotations and analyses are available at http://www-genome.wi.mit.edu/.]..

Research Grants3

  1. Pyrrolysine, a novel genetically encoded amino acid
    Joseph Krzycki; Fiscal Year: 2007
    ..Finally, transposon mutagenesis will be used to identify unknown genes with anticipated or unanticipated functions during UAG translation as pyrrolysine. ..