Joseph KrzyckiSummaryAffiliation: The Ohio State University Country: USA Publications
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Publications
Pyrrolysine encoded by UAG in Archaea: charging of a UAG-decoding specialized tRNAGayathri Srinivasan
Department of Microbiology, Ohio State University, Columbus, OH 43210, USA
Science 296:1459-62. 2002..Charging a tRNA(CUA) with lysine is a likely first step in translating UAG amber codons as pyrrolysine in certain methanogens. Our results indicate that pyrrolysine is the 22nd genetically encoded natural amino acid...- Function of genetically encoded pyrrolysine in corrinoid-dependent methylamine methyltransferasesJoseph A Krzycki
Department of Microbiology, OSU Biochemistry Program, Ohio State University, Columbus, Ohio 43210, USA
Curr Opin Chem Biol 8:484-91. 2004..This reaction is essential for methane formation from methylamines, and may underlie the retention of pyrrolysine in the genetic code of methanogens... - The direct genetic encoding of pyrrolysineJoseph A Krzycki
Department of Microbiology, OSU Biochemistry Program, Ohio State University, Columbus, OH 43210, USA
Curr Opin Microbiol 8:706-12. 2005..Thus, the recruitment of pyrrolysine into the genetic code involved evolution of the first non-canonical aminoacyl-tRNA synthetase and cognate tRNA to be described from nature... - Direct charging of tRNA(CUA) with pyrrolysine in vitro and in vivoSherry K Blight
Department of Microbiology, 484 West 12th Avenue, The Ohio State University, Columbus, Ohio 43210, USA
Nature 431:333-5. 2004..This is the first example from nature of direct aminoacylation of a tRNA with a non-canonical amino acid and shows that the genetic code of E. coli can be expanded to include UAG-directed pyrrolysine incorporation into proteins... - Characterization of a Methanosarcina acetivorans mutant unable to translate UAG as pyrrolysineAnirban Mahapatra
Department of Microbiology, Ohio State University, 484 West 12th Avenue, Columbus, OH 43210, USA
Mol Microbiol 59:56-66. 2006..These results indicate that in-depth genetic analysis of UAG translation as pyrrolysine is feasible, as deletion of pylT is conditionally lethal depending on growth substrate... - The residue mass of L-pyrrolysine in three distinct methylamine methyltransferasesJitesh A Soares
Department of Microbiology, Ohio State University, Columbus, Ohio 43210, USA
J Biol Chem 280:36962-9. 2005.... - A new UAG-encoded residue in the structure of a methanogen methyltransferaseBing Hao
Department of Biochemistry, The Ohio State University, 484 West 12th Avenue, Columbus, OH 43210, USA
Science 296:1462-6. 2002..Instead it appears consistent with a lysine in amide-linkage to (4R,5R)-4-substituted-pyrroline-5-carboxylate. We suggest that this amino acid be named l-pyrrolysine... - Reactivity and chemical synthesis of L-pyrrolysine- the 22(nd) genetically encoded amino acidBing Hao
Department of Biochemistry, The Ohio State University, Columbus, OH 43210, USA
Chem Biol 11:1317-24. 2004..Future applications of this synthetic L-pyrrolysine include its in vivo incorporation into recombinant proteins... 
RamA, a protein required for reductive activation of corrinoid-dependent methylamine methyltransferase reactions in methanogenic archaeaTsuneo Ferguson
Department of Microbiology and The Ohio State Biochemistry Program, Ohio State University, Columbus, Ohio 43210, USA
J Biol Chem 284:2285-95. 2009..These results suggest that RamA mediates reductive activation of corrinoid proteins and that it is the first functional archetype of COG3894, a family of redox proteins of unknown function...- A natural genetic code expansion cassette enables transmissible biosynthesis and genetic encoding of pyrrolysineDavid G Longstaff
Department of Microbiology, Campus Chemical Instrument Center Mass Spectrometry and Proteomics Facility, Ohio State University, 484 West 12th Avenue, Columbus, OH 43210, USA
Proc Natl Acad Sci U S A 104:1021-6. 2007..Analogous cassettes may have served similar functions for other amino acids during the evolutionary expansion of the canonical genetic code... 
Specificity of pyrrolysyl-tRNA synthetase for pyrrolysine and pyrrolysine analogsWen Tai Li
Department of Chemistry, The Ohio State University, Columbus, OH 43210, USA
J Mol Biol 385:1156-64. 2009....- Class I and class II lysyl-tRNA synthetase mutants and the genetic encoding of pyrrolysine in Methanosarcina sppAnirban Mahapatra
Department of Microbiology, Ohio State University, 484 West 12th Avenue, Columbus, OH 43210, USA
Mol Microbiol 64:1306-18. 2007..However, decreased lysyl-tRNA(Lys) in the lysS mutant provides a possible rationale for stable transfer of the bacterial lysS gene to methanoarchaea... - The genome of M. acetivorans reveals extensive metabolic and physiological diversityJames E Galagan
Whitehead Institute Center for Genome Research, Cambridge, Massachusetts 02141, USA
Genome Res 12:532-42. 2002..acetivorans a powerful model organism for the study of archaeal biology. [Sequence, data, annotations and analyses are available at http://www-genome.wi.mit.edu/.]..
Research Grants
- Pyrrolysine, a novel genetically encoded amino acidJoseph Krzycki; Fiscal Year: 2007..Finally, transposon mutagenesis will be used to identify unknown genes with anticipated or unanticipated functions during UAG translation as pyrrolysine. ..