Research Topics
| Maria M KonarskaSummaryAffiliation: The Rockefeller University Country: USA Publications
| Collaborators
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Detail Information
Publications
Repositioning of the reaction intermediate within the catalytic center of the spliceosomeMaria M Konarska
The Rockefeller University, New York, New York 10021, USA
Mol Cell 21:543-53. 2006..Changes in the relative occupancy of first and second step substrates at the catalytic center alter efficiency of the two steps of splicing, allowing use of suboptimal intron sequences and thereby altering substrate selectivity...- Insights into the mechanisms of splicing: more lessons from the ribosomeMaria M Konarska
The Rockefeller University, New York, New York 10021, USA
Genes Dev 19:2255-60. 2005 
Insights into branch nucleophile positioning and activation from an orthogonal pre-mRNA splicing system in yeastDuncan J Smith
The Rockefeller University, New York, NY 10065, USA
Mol Cell 34:333-43. 2009..These results provide insights into substrate selection during spliceosomal branching catalysis; additionally, this system provides a foundation and tool for future mechanistic splicing research...- trans-splicing to spliceosomal U2 snRNA suggests disruption of branch site-U2 pairing during pre-mRNA splicingDuncan J Smith
The Rockefeller University, New York, NY 10021, USA
Mol Cell 26:883-90. 2007..Multiple spatially disparate splice sites in U2 can be used, calling into question both the fate of its pairing to the branch site and the details of its role in splicing catalysis... - "Nought may endure but mutability": spliceosome dynamics and the regulation of splicingDuncan J Smith
The Rockefeller University, New York, NY 10065, USA
Mol Cell 30:657-66. 2008..Each transition along the splicing pathway presents an opportunity for progression, pausing, or discard, allowing splice site choice to be regulated throughout both the assembly and catalytic phases of the reaction... - Opposing classes of prp8 alleles modulate the transition between the catalytic steps of pre-mRNA splicingLi Liu
The Rockefeller University, New York, New York 10021, USA
Nat Struct Mol Biol 14:519-26. 2007..Modulation of these events alters splice-site selection and splicing fidelity... - Identification and characterization of a short 2'-3' bond-forming ribozymeDuncan J Smith
Laboratory of Molecular Biology and Biochemistry, The Rockefeller University, New York, New York 10065, USA
RNA 15:8-13. 2009..Our data have implications for the intrinsic reactivity of the large amount of RNA sequence space known to be transcribed in nature and for the validity and utility of the use of protein-free systems to study pre-mRNA splicing... - A critical assessment of the utility of protein-free splicing systemsDuncan J Smith
Laboratory of Molecular Biology and Biochemistry, The Rockefeller University, New York, New York 10065, USA
RNA 15:1-3. 2009.... - Mechanistic insights from reversible splicing catalysisDuncan J Smith
The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
RNA 14:1975-8. 2008.... - A sequence motif in the simian virus 40 (SV40) early core promoter affects alternative splicing of transcribed mRNAElisenda Gendra
Rockefeller University, New York, New York 10021, USA
J Biol Chem 282:11648-57. 2007.... - p54(nrb) associates with the 5' splice site within large transcription/splicing complexesSei Kameoka
The Rockefeller University, New York, NY 10021, USA
EMBO J 23:1782-91. 2004.... - Spliceosomal snRNAs in the unicellular eukaryote Trichomonas vaginalis are structurally conserved but lack a 5'-cap structureAugusto Simoes-Barbosa
Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, California 90095 1489, USA
RNA 14:1617-31. 2008..Despite the lack of a cap structure, U1, U2, U4, and U5 genes are transcribed by RNA polymerase II, whereas the U6 gene is transcribed by RNA polymerase III... - Suppression of multiple substrate mutations by spliceosomal prp8 alleles suggests functional correlations with ribosomal ambiguity mutantsCharles C Query
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
Mol Cell 14:343-54. 2004..This mechanistic commonality suggests that alteration of rearrangements represents an evolutionarily convenient way of modulating substrate selectivity... - A purified catalytically competent spliceosomeMaria M Konarska
Nat Struct Mol Biol 15:222-4. 2008 - Splicing fidelity revisitedCharles C Query
Nat Struct Mol Biol 13:472-4. 2006 - Prp5 bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNAYong Zhen Xu
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY, USA
EMBO J 23:376-85. 2004..pombe. Together, these data are consistent with Prp5 being a bridge between U1 and U2 snRNPs at the time of pre-spliceosome formation...