Research Topics
| GERALD JOYCESummaryAffiliation: The Scripps Research Institute Country: USA Publications
Research Grants
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Detail Information
Publications
The antiquity of RNA-based evolutionGerald F Joyce
Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Nature 418:214-21. 2002..This earlier era is referred to as the 'RNA world', during which the genetic information resided in the sequence of RNA molecules and the phenotype derived from the catalytic properties of RNA...
Bit by bit: the Darwinian basis of lifeGerald F Joyce
Department of Molecular Biology, Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California, United States of America
PLoS Biol 10:e1001323. 2012....- Evolution in an RNA worldG F Joyce
Departments of Chemistry and Molecular Biology and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Cold Spring Harb Symp Quant Biol 74:17-23. 2009..This is the first example, outside of biology, of evolutionary adaptation in a molecular genetic system... - The implausibility of metabolic cycles on the prebiotic EarthLeslie E Orgel
The Salk Institute for BiologicalStudies, San Diego, California, United States ofAmerica
PLoS Biol 6:e18. 2008 - Forty years of in vitro evolutionGerald F Joyce
Department of Chemistry and Molecular Biology, La Jolla, CA 92037, USA
Angew Chem Int Ed Engl 46:6420-36. 2007..This Review summarizes the concepts and methods for the directed evolution of RNA molecules in vitro... - Structural biology. A glimpse of biology's first enzymeGerald F Joyce
Departments of Chemistry and of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037, USA
Science 315:1507-8. 2007 - The promise and peril of continuous in vitro evolutionGlenn C Johns
Departments of Chemistry and Molecular Biology and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
J Mol Evol 61:253-63. 2005..It offers a candid appraisal of both the strengths and the limitations of these systems... - A complex ligase ribozyme evolved in vitro from a group I ribozyme domainL Jaeger
Institut de Biologie Moleculaire et Cellulaire du Centre National de la Recherche Scientifique, 15 rue Descartes, 67084 Strasbourg, France
Proc Natl Acad Sci U S A 96:14712-7. 1999..This work demonstrates that complex RNA molecules, like their protein counterparts, can share common structural domains while exhibiting distinct catalytic functions... - Directed evolution of nucleic acid enzymesGerald F Joyce
Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, USA
Annu Rev Biochem 73:791-836. 2004..The difficulty of this reaction, and therefore the complexity of the corresponding DNA enzyme, depends on whether a catalytic cofactor, such as a divalent metal cation or small molecule, is present in the reaction mixture... - Self-replicationNatasha Paul
Department of Chemistry, Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, 92037, La Jolla, CA, USA
Curr Biol 13:R46. 2003 - RNA structure. Ribozyme evolution at the crossroadsG F Joyce
Department of Chemistry, Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Science 289:401-2. 2000..In a remarkable study (Schultes and Bartel), an RNA sequence has been designed that can adopt two different structures, each with a different catalytic function. Joyce details how this study sheds light on the evolution of enzymes... - A ribozyme composed of only two different nucleotidesJohn S Reader
Department of Chemistry, The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
Nature 420:841-4. 2002..This ribozyme is specific for the formation of biologically relevant 3',5'-phosphodiester linkages... - Emergence of a fast-reacting ribozyme that is capable of undergoing continuous evolutionSarah B Voytek
Department of Chemistry, The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 104:15288-93. 2007..Now that two distinct species of continuously evolving enzymes have been established, it is possible to conduct molecular ecology experiments in which the two are made to compete for limited resources within a common environment... - Obituary: Leslie Orgel (1927-2007)Gerald F Joyce
Gerald F. Joyce is in the Departments of Chemistry and Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037
Nature 450:627. 2007 - A 1.7-kilobase single-stranded DNA that folds into a nanoscale octahedronWilliam M Shih
Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
Nature 427:618-21. 2004..Because the base-pair sequence of individual struts is not repeated in a given octahedron, each strut is uniquely addressable by the appropriate sequence-specific DNA binder... - A modular, bifunctional RNA that integrates itself into a target RNARoshan M Kumar
Department of Chemistry, Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 100:9738-43. 2003..These molecules also may be useful as tools for the insertional mutagenesis of target mRNAs... - Microfluidic compartmentalized directed evolutionBrian M Paegel
Department of Chemistry, The Scripps Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA
Chem Biol 17:717-24. 2010..During five rounds of this procedure, the enzymes acquired mutations that conferred resistance to neomycin and caused some enzymes to become dependent on neomycin for optimal activity... - Substitution of ribonucleotides in the T7 RNA polymerase promoter elementKathleen E McGinness
Department of Chemistry, The Skaggs Institute for Chemical Biology, La Jolla, California 92037, USA
J Biol Chem 277:2987-91. 2002.... - Self-sustained replication of an RNA enzymeTracey A Lincoln
Department of Chemistry and Skaggs Institute for Chemical Biology, Scripps Research Institute, La Jolla, CA 92037, USA
Science 323:1229-32. 2009..These replicating RNA enzymes can serve as an experimental model of a genetic system. Many such model systems could be constructed, allowing different selective outcomes to be related to the underlying properties of the genetic system... - RNA-catalyzed RNA ligation on an external RNA templateKathleen E McGinness
Department of Chemistry, The Skaggs Institute for Chemical Biology, La Jolla, CA 92037, USA
Chem Biol 9:297-307. 2002..The development of ribozymes that catalyze polymerase-type reactions contributes to the notion that an RNA world could have existed during the early history of life on Earth... - Continuous in vitro evolution of a ribozyme that catalyzes three successive nucleotidyl addition reactionsKathleen E McGinness
Department of Chemistry and The Skaggs Institute for Chemical Biology, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Chem Biol 9:585-96. 2002..This purely chemical system provides a functional model of a multi-step reaction pathway that is undergoing Darwinian evolution... - Conversion of a ribozyme to a deoxyribozyme through in vitro evolutionNatasha Paul
Department of Chemistry, The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
Chem Biol 13:329-38. 2006..This transfer of both information and function is relevant to the transition between two different genetic systems based on nucleic acid-like molecules, as postulated to have occurred during the early history of life on Earth... - Minimal self-replicating systemsNatasha Paul
Departments of Chemistry and Molecular Biology and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA
Curr Opin Chem Biol 8:634-9. 2004..This review analyzes chemical self-replicating systems that have been developed to date and discusses ongoing challenges in this area of research... - A DNA-templated aldol reaction as a model for the formation of pentose sugars in the RNA worldMichael Oberhuber
Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA
Angew Chem Int Ed Engl 44:7580-3. 2005 - Niche partitioning in the coevolution of 2 distinct RNA enzymesSarah B Voytek
Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 106:7780-5. 2009.... - Autocatalytic aptazymes enable ligand-dependent exponential amplification of RNABianca J Lam
Department of Chemistry, The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
Nat Biotechnol 27:288-92. 2009..This process is analogous to quantitative PCR (qPCR) but can be generalized to a wide variety of targets, including proteins and small molecules that are relevant to medical diagnostics and environmental monitoring... - Perfectly complementary nucleic acid enzymesScott T Kuhns
Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
J Mol Evol 56:711-7. 2003..The ability to generate functional nucleic acids encoded by both strands of a duplex has implications for the evolution of catalytic nucleic acids and the prospects for realizing maximum functionality from a given genetic sequence... - Continuous in vitro evolution of ribozymes that operate under conditions of extreme pHHenriette Kühne
Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
J Mol Evol 57:292-8. 2003..The results establish continuous in vitro evolution as a useful model system for exploring the evolution of enzymatic function in extreme environments... - RNA-cleaving DNA enzymes with altered regio- or enantioselectivityPhillip Ordoukhanian
Departments of Chemistry, The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, USA
J Am Chem Soc 124:12499-506. 2002..These molecules demonstrate how in vitro evolution can be used to obtain regio- and enantioselective catalysts that exhibit specificities for nonnatural analogues of biological compounds... - A self-replicating ligase ribozymeNatasha Paul
Departments of Chemistry and Molecular Biology and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 99:12733-40. 2002..Further optimization of the system may lead to the sustained exponential growth of ribozymes that undergo self-replication... - In search of an RNA replicase ribozymeKathleen E McGinness
Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Chem Biol 10:5-14. 2003.... - Darwinian evolution on a chipBrian M Paegel
Department of Chemistry, The Scripps Research Institute, La Jolla, California, United States of America
PLoS Biol 6:e85. 2008..This system reduces evolution to a microfluidic algorithm, allowing the experimenter to observe and manipulate adaptation... - Cross-catalytic replication of an RNA ligase ribozymeDong-Eun Kim
Department of Chemistry and Molecular Biology and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Chem Biol 11:1505-12. 2004..As the concentration of the two ribozymes increases, the rate of formation of additional ribozyme molecules increases, consistent with the overall autocatalytic behavior of the reaction system... - Molecular evolution: booting up lifeGerald F Joyce
Nature 420:278-9. 2002
Research Grants
- Directed Evolution of Nucleic Acid EnzymesGERALD JOYCE; Fiscal Year: 2005..The adapter will allow the cofactor to be bound readily by the enzyme, allowing evolution to exploit the bound cofactor for use in catalysis. ..
- Directed Evolution of Nucleic Acid EnzymesGERALD JOYCE; Fiscal Year: 2007..These decorated nanostructures then will be subject to in vitro evolution, selecting on the basis of both their form and the function of the appended DNAs. ..
- Directed Evolution of Nucleic Acid EnzymesGerald F Joyce; Fiscal Year: 2010..These decorated nanostructures then will be subject to in vitro evolution, selecting on the basis of both their form and the function of the appended DNAs. ..