Affiliation: The Rockefeller University
- Ordered ATP hydrolysis in the gamma complex clamp loader AAA+ machineAaron Johnson
Howard Hughes Medical Institute and The Rockefeller University, New York, New York 10021, USA
J Biol Chem 278:14406-13. 2003..Implications of these results to clamp loaders of other systems are discussed...
- In vivo magnetic resonance imaging of immune cells in the central nervous system with superparamagnetic antibodiesIstvan Pirko
Department of Neurology, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA
FASEB J 18:179-82. 2004..Because superparamagnetic antibodies similar to those used in the study are approved for human use, the in vivo MRI technique we have described could be developed for human use...
- Cellular DNA replicases: components and dynamics at the replication forkAaron Johnson
Howard Hughes Medical Institute, New York City, New York 10021 6399, USA
Annu Rev Biochem 74:283-315. 2005..Twin polymerases and clamps coordinate their actions with a clamp loader and yet other proteins to form a replisome machine that advances the replication fork...
- DNA ligase: getting a grip to seal the dealAaron Johnson
The Rockefeller University 1230 York Avenue, Box 228, New York, New York 10021 6399, USA
Curr Biol 15:R90-2. 2005..The elegant structure explains how ligase attains fidelity for the sealing operation...
- Characterization of a triple DNA polymerase replisomePeter McInerney
Laboratory of DNA Replication, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA
Mol Cell 27:527-38. 2007..We propose that two polymerases can function on the lagging strand and that the third DNA polymerase can act as a reserve enzyme to overcome certain types of obstacles to the replication fork...
- The replication factor C clamp loader requires arginine finger sensors to drive DNA binding and proliferating cell nuclear antigen loadingAaron Johnson
Laboratory of DNA Replication, Howard Hughes Medical Institute and Rockefeller University, New York, New York 10021, USA
J Biol Chem 281:35531-43. 2006..PCNA closure severs contact to RFC subunits D and E (RFC2 and RFC5), and the gamma-phosphate sensor of ATP site C is switched off, resulting in low affinity of RFC for DNA and ejection of RFC from the site of PCNA loading...
- Fluorescence measurements on the E.coli DNA polymerase clamp loader: implications for conformational changes during ATP and clamp bindingEric R Goedken
Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA
J Mol Biol 336:1047-59. 2004....
- Mechanism of loading the Escherichia coli DNA polymerase III sliding clamp: II. Uncoupling the beta and DNA binding activities of the gamma complexAnita K Snyder
Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, Florida 32610 0245, USA
J Biol Chem 279:4386-93. 2004..Additionally, these results show that the "arginine fingers" play a structural role in facilitating the formation of a conformation that has high affinity for beta and DNA...
- Mechanism of proliferating cell nuclear antigen clamp opening by replication factor CNina Y Yao
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
J Biol Chem 281:17528-39. 2006..Interestingly, the Rad.RFC DNA damage checkpoint clamp loader unloads PCNA clamps from DNA. We propose that Rad.RFC may clear PCNA from DNA to facilitate shutdown of replication in the face of DNA damage...
- Motion of a DNA sliding clamp observed by single molecule fluorescence spectroscopyTed A Laurence
Lawrence Livermore National Laboratory, California 94550, USA
J Biol Chem 283:22895-906. 2008..These results may imply that the clamp not only acts to hold the polymerase on the DNA but also prevents excessive drifting along the DNA...