JAMES ALFRED HOCH

Summary

Affiliation: The Scripps Research Institute
Country: USA

Publications

  1. ncbi request reprint Initiation of bacterial development
    J A Hoch
    The Scripps Research Institute, Molecular and Experimental Medicine, Division of Cellular Biology, 10550 North Torrey Pines Road, NX 1, La Jolla, CA 92037, USA
    Curr Opin Microbiol 1:170-4. 1998
  2. ncbi request reprint Two-component and phosphorelay signal transduction
    J A Hoch
    Division of Cellular Biology, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    Curr Opin Microbiol 3:165-70. 2000
  3. pmc A novel histidine kinase inhibitor regulating development in Bacillus subtilis
    L Wang
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    Genes Dev 11:2569-79. 1997
  4. ncbi request reprint Crystal structure of a phosphatase-resistant mutant of sporulation response regulator Spo0F from Bacillus subtilis
    - Madhusudan
    Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, CA 92037, USA
    Structure 4:679-90. 1996
  5. ncbi request reprint Multisensory activation of the phosphorelay initiating sporulation in Bacillus subtilis: identification and sequence of the protein kinase of the alternate pathway
    K A Trach
    Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, California 92037
    Mol Microbiol 8:69-79. 1993
  6. ncbi request reprint Alterations in the flow of one-carbon units affect KinB-dependent sporulation in Bacillus subtilis
    V Dartois
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    Mol Microbiol 25:39-51. 1997
  7. ncbi request reprint Phosphorylation of the Spo0B response regulator phosphotransferase of the phosphorelay initiating development in Bacillus subtilis
    Y L Tzeng
    Division of Cellular Biology, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 273:23849-55. 1998
  8. pmc Characterization of the gene for a protein kinase which phosphorylates the sporulation-regulatory proteins Spo0A and Spo0F of Bacillus subtilis
    M Perego
    Department of Molecular and Experimental Medicine, Research Institute of Scripps Clinic, La Jolla, California 92037
    J Bacteriol 171:6187-96. 1989
  9. ncbi request reprint Formation of a novel four-helix bundle and molecular recognition sites by dimerization of a response regulator phosphotransferase
    K I Varughese
    Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, California 92037, USA
    Mol Cell 2:485-93. 1998
  10. ncbi request reprint KapB is a lipoprotein required for KinB signal transduction and activation of the phosphorelay to sporulation in Bacillus subtilis
    V Dartois
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Microbiol 26:1097-108. 1997

Research Grants

  1. Signal Transduction Networks in Bacillus anthracis
    JAMES ALFRED HOCH; Fiscal Year: 2010
  2. GENETIC CONTROL OF DEVELOPMENT
    JAMES HOCH; Fiscal Year: 2007
  3. Signal Transduction Networks in Bacillus anthracis
    JAMES HOCH; Fiscal Year: 2007
  4. Signal Transduction Networks in Bacillus anthracis
    JAMES HOCH; Fiscal Year: 2006
  5. GENETIC CONTROL OF DEVELOPMENT
    JAMES HOCH; Fiscal Year: 2005
  6. GENETIC CONTROL OF DEVELOPMENT
    JAMES HOCH; Fiscal Year: 1991
  7. GENETIC CONTROL OF DEVELOPMENT
    JAMES ALFRED HOCH; Fiscal Year: 2010

Collaborators

  • M Perego
  • K I Varughese
  • V Dartois
  • Li Wang
  • S P Cole
  • Keith Stephenson
  • Hendrik Szurmant
  • John Cavanagh
  • C L Brooks
  • Malcolm Winkler
  • Christopher F Higgins
  • Adam C Wilson
  • Christina Chiang
  • Tatsuya Fukushima
  • Robert A White
  • Eugenio Santelli
  • Patrick D McLaughlin
  • - Madhusudan
  • Billyana Tsvetanova
  • J Zapf
  • Richele J Thompson
  • Kristina Wörner
  • Andrea K White
  • Ryan L Brunsing
  • Douglas J Kojetin
  • A Koide
  • Haiyan Zhao
  • Eun Ja Kim
  • Erin J Regel
  • Michael A Mohan
  • Benjamin G Bobay
  • Terence Hwa
  • Robert C Liddington
  • Cristina Bongiorni
  • C Fabret
  • Adam Godzik
  • Y L Tzeng
  • Marcin Grynberg
  • Chandra La Clair
  • Stephen Naylor
  • Charles H Opperman
  • Sharon Tang
  • Linda M Benson
  • Lynn E Hancock
  • Keith G Davies
  • Jenora Waterman
  • M Madhusudan
  • N H Xuong
  • J M Whiteley
  • Tarek Msadek
  • James Zapf
  • U Sen
  • X Z Zhou
  • K A Trach
  • M A Strauch
  • P T Kallio
  • B E Van Hoy
  • L R Faust
  • J A Connor
  • D M Roof
  • D Burbulys
  • W C Johnson
  • B M Babior
  • J E Fagelson
  • G B Spiegelman

Detail Information

Publications51

  1. ncbi request reprint Initiation of bacterial development
    J A Hoch
    The Scripps Research Institute, Molecular and Experimental Medicine, Division of Cellular Biology, 10550 North Torrey Pines Road, NX 1, La Jolla, CA 92037, USA
    Curr Opin Microbiol 1:170-4. 1998
    ..New mechanisms of developmental regulation are being uncovered in a variety of microbial systems...
  2. ncbi request reprint Two-component and phosphorelay signal transduction
    J A Hoch
    Division of Cellular Biology, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    Curr Opin Microbiol 3:165-70. 2000
    ..Elucidation of the structures of the interacting domains is leading to an understanding of the mechanisms of molecular recognition and phosphotransfer in these systems...
  3. pmc A novel histidine kinase inhibitor regulating development in Bacillus subtilis
    L Wang
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    Genes Dev 11:2569-79. 1997
    ....
  4. ncbi request reprint Crystal structure of a phosphatase-resistant mutant of sporulation response regulator Spo0F from Bacillus subtilis
    - Madhusudan
    Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, CA 92037, USA
    Structure 4:679-90. 1996
    ....
  5. ncbi request reprint Multisensory activation of the phosphorelay initiating sporulation in Bacillus subtilis: identification and sequence of the protein kinase of the alternate pathway
    K A Trach
    Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, California 92037
    Mol Microbiol 8:69-79. 1993
    ..The KinA and KinB pathways are the only pathways capable of producing sufficient Spo0A approximately P to allow initiation and completion of sporulation under laboratory conditions...
  6. ncbi request reprint Alterations in the flow of one-carbon units affect KinB-dependent sporulation in Bacillus subtilis
    V Dartois
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    Mol Microbiol 25:39-51. 1997
    ..Our results indicate that lrpA-lrpB together with the biosynthetic glyA gene lie on a common signalling pathway meant to activate the KinB sensor kinase...
  7. ncbi request reprint Phosphorylation of the Spo0B response regulator phosphotransferase of the phosphorelay initiating development in Bacillus subtilis
    Y L Tzeng
    Division of Cellular Biology, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 273:23849-55. 1998
    ....
  8. pmc Characterization of the gene for a protein kinase which phosphorylates the sporulation-regulatory proteins Spo0A and Spo0F of Bacillus subtilis
    M Perego
    Department of Molecular and Experimental Medicine, Research Institute of Scripps Clinic, La Jolla, California 92037
    J Bacteriol 171:6187-96. 1989
    ..The results suggest that multiple kinases exist for the Spo0A protein...
  9. ncbi request reprint Formation of a novel four-helix bundle and molecular recognition sites by dimerization of a response regulator phosphotransferase
    K I Varughese
    Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, California 92037, USA
    Mol Cell 2:485-93. 1998
    ..It is apparent that different surfaces of response regulators may be involved in recognition of the protein partners to which they are paired...
  10. ncbi request reprint KapB is a lipoprotein required for KinB signal transduction and activation of the phosphorelay to sporulation in Bacillus subtilis
    V Dartois
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Microbiol 26:1097-108. 1997
    ..Models for the role of KapB in regulating KinB activity are discussed...
  11. ncbi request reprint Protein aspartate phosphatases control the output of two-component signal transduction systems
    M Perego
    Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, CA 92037, USA
    Trends Genet 12:97-101. 1996
    ..Such phosphatases are prevalent in signal transduction systems controlling complex processes, such as the initiation of development in microorganisms...
  12. pmc Identification of a membrane protein involved in activation of the KinB pathway to sporulation in Bacillus subtilis
    V Dartois
    Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, California 92037, USA
    J Bacteriol 178:1178-86. 1996
    ..Implications of these observations on the transcriptional regulation of kinB and the role of KbaA in KinB activation are discussed...
  13. pmc Dissection of the functional and structural domains of phosphorelay histidine kinase A of Bacillus subtilis
    L Wang
    Division of Cellular Biology, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Bacteriol 183:2795-802. 2001
    ..The N-terminal region appears to be essential both for signal sensing and for maintaining the correct conformation of the autokinase component domains...
  14. pmc Characterization of the spoIVB and recN loci of Bacillus subtilis
    B E Van Hoy
    Department of Molecular and Experimental Medicine, Research Institute of Scripps Clinic, La Jolla, California 92037
    J Bacteriol 172:1306-11. 1990
    ..subtilis cells, as determined by beta-galactosidase activity assays from lacZ transcriptional fusion constructions. We found no amino acid sequence homology between the spoIVB gene product and other known bacterial proteins...
  15. ncbi request reprint A response regulatory protein with the site of phosphorylation blocked by an arginine interaction: crystal structure of Spo0F from Bacillus subtilis
    M Madhusudan
    Department of Biology, University of California at San Diego, La Jolla, California 92093 0359, USA
    Biochemistry 36:12739-45. 1997
    ..This observation suggests that a small molecule with a shape complementary to the active site and having a guanidinium group might inhibit phosphotransfer between response regulators and their cognate histidine kinases...
  16. pmc ScoC regulates peptide transport and sporulation initiation in Bacillus subtilis
    A Koide
    Division of Cellular Biology, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Bacteriol 181:4114-7. 1999
    ..Since peptide transport is essential for inactivation of the negative regulation of sporulation by Rap phosphatases, the control of ScoC transcription repression activity plays a crucial role in the initiation of sporulation...
  17. ncbi request reprint Isolation and sequence of the spo0E gene: its role in initiation of sporulation in Bacillus subtilis
    M Perego
    Division of Cellular Biology, Research Insititute of Scripps Clinic, La Jolla, CA 92037
    Mol Microbiol 1:125-32. 1987
    ..Studies of the regulation of this gene have allowed the generation of a hypothesis to explain the interactions of those five stage 0 genes involved in the activation of sporulation-specific transcription...
  18. pmc PAS-A domain of phosphorelay sensor kinase A: a catalytic ATP-binding domain involved in the initiation of development in Bacillus subtilis
    K Stephenson
    Division of Cellular Biology, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 98:15251-6. 2001
    ....
  19. ncbi request reprint A transient interaction between two phosphorelay proteins trapped in a crystal lattice reveals the mechanism of molecular recognition and phosphotransfer in signal transduction
    J Zapf
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    Structure 8:851-62. 2000
    ..Information at the molecular level on the interaction between response regulators and phosphohistidine domains is necessary to develop a rationale for how phospho-signaling fidelity is maintained in two-component systems...
  20. ncbi request reprint Structure of the gene for the transition state regulator, abrB: regulator synthesis is controlled by the spo0A sporulation gene in Bacillus subtilis
    M Perego
    Division of Cellular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037
    Mol Microbiol 2:689-99. 1988
    ..The level of this regulator is, in turn, controlled by the spo0A gene. The pleiotropic phenotypes of spo0A mutants result from uncontrolled overexpression of the abrB regulator...
  21. pmc The transition state transcription regulator abrB of Bacillus subtilis is a DNA binding protein
    M A Strauch
    Department of Basic and Clinical Research, Research Institute of Scripps Clinic, La Jolla, CA 92037
    EMBO J 8:1615-21. 1989
    ..The results suggested that stationary phase functions and sporulation are repressed during active growth by AbrB and other transition state regulators by binding to the affected promoters in a concentration-dependent manner...
  22. ncbi request reprint The oligopeptide transport system of Bacillus subtilis plays a role in the initiation of sporulation
    M Perego
    Department of Molecular and Experimental Medicine, Research Institute of Scripps Clinic, La Jolla, California 92037
    Mol Microbiol 5:173-85. 1991
    ..We postulate that the accumulation of such peptides may play a signalling role in the initiation of sporulation, and that the sporulation defect in opp mutants results from an inability to transport these peptides...
  23. ncbi request reprint Identification of a second oligopeptide transport system in Bacillus subtilis and determination of its role in sporulation
    A Koide
    Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, California 92037
    Mol Microbiol 13:417-26. 1994
    ..The repaired App system can substitute completely for the Opp system in both sporulation and competence for genetic transformation. The phenotypes raised some speculation about the subunit configuration of the Opp system...
  24. ncbi request reprint Histidine kinase-mediated signal transduction systems of pathogenic microorganisms as targets for therapeutic intervention
    K Stephenson
    Division of Cellular Biology, Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, CA 92037, USA
    Curr Drug Targets Infect Disord 2:235-46. 2002
    ..In addition, the current status on the development of inhibitors specific for two-component systems will be discussed...
  25. pmc A two-component signal transduction system essential for growth of Bacillus subtilis: implications for anti-infective therapy
    C Fabret
    Division of Cellular Biology, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, USA
    J Bacteriol 180:6375-83. 1998
    ..The high level of identity of these proteins (71% for YycF) suggests that this system may play a similar role in gram-positive pathogens...
  26. ncbi request reprint The transition state regulator Hpr of Bacillus subtilis is a DNA-binding protein
    P T Kallio
    Department of Molecular and Experimental Medicine, Research Institute of Scripps Clinic, La Jolla, California 92037
    J Biol Chem 266:13411-7. 1991
    ..However, in mixing competition experiments we have shown that Hpr and Sin binding occurred independently, and no visible alterations of protected regions were detected...
  27. ncbi request reprint Cloning, sequencing, and expression of the genes encoding the adenosylcobalamin-dependent ethanolamine ammonia-lyase of Salmonella typhimurium
    L R Faust
    Department of Molecular and Experimental Medicine, Research Institute of Scripps Clinic, La Jolla, California 92037
    J Biol Chem 265:12462-6. 1990
    ..coli ethanolamine ammonia-lyase, except that residue 16 of the large subunit was asparagine in the E. coli sequence and aspartic acid in the S. typhimurium sequence...
  28. pmc YycH and YycI interact to regulate the essential YycFG two-component system in Bacillus subtilis
    Hendrik Szurmant
    Division of Cellular Biology, Mail Code MEM 116, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Bacteriol 189:3280-9. 2007
    ..The data suggest that YycH and YycI control the activity of YycG in the periplasm and that this control is crucial in regulating important cellular processes...
  29. pmc Predominantly buried residues in the response regulator Spo0F influence specific sensor kinase recognition
    Patrick D McLaughlin
    Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC 27695, USA
    FEBS Lett 581:1425-9. 2007
    ..In addition, we discuss how buried residues and intra-protein communication networks contribute to precise molecular recognition by ensuring that the correct surface is presented...
  30. ncbi request reprint The essential YycFG two-component system of Bacillus subtilis
    Hendrik Szurmant
    Department of Molecular and Structural Biochemistry, North Carolina State University, North Carolina, USA
    Methods Enzymol 422:396-417. 2007
    ..Most aspects of our approaches are applicable to other two-component systems in B. subtilis and the gram positives. Some are limited to essential systems, such as the YycFG system...
  31. pmc Sensor complexes regulating two-component signal transduction
    Hendrik Szurmant
    The Scripps Research Institute, Division of Cellular Biology, Mail Code MEM 116, Department of Molecular and Experimental Medicine, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Curr Opin Struct Biol 17:706-15. 2007
    ..Structural studies of such domains are leading to an understanding of the mechanisms by which sensor complexes recognize signals and regulate kinase activity...
  32. pmc New transposon delivery plasmids for insertional mutagenesis in Bacillus anthracis
    Adam C Wilson
    The Scripps Research Institute, Department of Molecular and Experimental, Medicine, Division of Cellular Biology, 10550 North Torrey Pines Road, La Jolla, CA 92037, United States
    J Microbiol Methods 71:332-5. 2007
    ..The utility of both systems was directly demonstrated through the mutagenesis of a widely used B. anthracis strain...
  33. pmc Essentiality, bypass, and targeting of the YycFG (VicRK) two-component regulatory system in gram-positive bacteria
    Malcolm E Winkler
    Department of Biology, Indiana University Bloomington, Jordan Hall, Room 142, Bloomington, IN 47405, USA
    J Bacteriol 190:2645-8. 2008
  34. doi request reprint Commingling regulatory systems following acquisition of virulence plasmids by Bacillus anthracis
    Marta Perego
    Division of Cellular Biology, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Trends Microbiol 16:215-21. 2008
    ..An example of such a conversion is seen in Bacillus anthracis, and how acquired plasmid regulatory functions affect the activity of the regulatory processes of the bacterium, and vice versa, is now being revealed...
  35. pmc An essential sensor histidine kinase controlled by transmembrane helix interactions with its auxiliary proteins
    Hendrik Szurmant
    Division of Cellular Biology, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 105:5891-6. 2008
    ....
  36. pmc Virulence gene expression is independent of ResDE-regulated respiration control in Bacillus anthracis
    Adam C Wilson
    Department of Molecular and Experimental Medicine, Division of Cellular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    J Bacteriol 190:5522-5. 2008
    ..The results indicate that the loss of ResDE-controlled respiratory capacity does not affect the synthesis of anthrax toxin...
  37. pmc A sensor histidine kinase co-ordinates cell wall architecture with cell division in Bacillus subtilis
    Tatsuya Fukushima
    Division of Cellular Biology, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA, USA
    Mol Microbiol 69:621-32. 2008
    ....
  38. pmc The crystal structure of Bacillus subtilis YycI reveals a common fold for two members of an unusual class of sensor histidine kinase regulatory proteins
    Eugenio Santelli
    Division of Cellular Biology, Mail Code MEM 116, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Bacteriol 189:3290-5. 2007
    ..9-A resolution. YycI forms a dimer, and remarkably the structure resembles that of the two C-terminal domains of YycH despite nearly undetectable sequence homology (10%) between the two proteins...
  39. ncbi request reprint Opposing effects of histidine phosphorylation regulate the AtxA virulence transcription factor in Bacillus anthracis
    Billyana Tsvetanova
    The Scripps Research Institute, Department of Molecular and Experimental Medicine, Division of Cellular Biology, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Mol Microbiol 63:644-55. 2007
    ..Our results link virulence factor production in B. anthracis to carbohydrate metabolism and, for the first time, provide a mechanistic explanation for AtxA transcriptional activity...
  40. ncbi request reprint DNA complexed structure of the key transcription factor initiating development in sporulating bacteria
    Haiyan Zhao
    Division of Cellular Biology, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    Structure 10:1041-50. 2002
    ..The protein:protein and protein:DNA interfaces revealed in the crystal provide a basis for interpreting the transcription activation process and for the design of drugs to counter infections by these bacteria...
  41. ncbi request reprint Evolution of signalling in the sporulation phosphorelay
    Keith Stephenson
    Department of Molecular and Experimental Medicine, The Scripps Research Insitute, La Jolla, CA 92037, USA
    Mol Microbiol 46:297-304. 2002
    ..In Clostridial genomes, only the Spo0A protein was found, suggesting that the anaerobic relatives of the Bacilli do not use a phosphorelay and phosphorylate Spo0A directly with sensor kinases...
  42. pmc Functional genomics of gram-positive microorganisms
    Marta Perego
    Division of Cellular Biology, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Bacteriol 186:903-9. 2004
  43. pmc YycH regulates the activity of the essential YycFG two-component system in Bacillus subtilis
    Hendrik Szurmant
    Division of Cellular Biology, Mail Code MEM 116, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Bacteriol 187:5419-26. 2005
    ....
  44. pmc Characterization of sporulation histidine kinases of Bacillus anthracis
    Ryan L Brunsing
    Division of Cellular Biology, Mail Code MEM 116, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Bacteriol 187:6972-81. 2005
    ..The sporulation of B. anthracis ex vivo appears to result from any one or a combination of the sporulation sensor histidine kinases remaining...
  45. ncbi request reprint Structural analysis of divalent metals binding to the Bacillus subtilis response regulator Spo0F: the possibility for in vitro metalloregulation in the initiation of sporulation
    Douglas J Kojetin
    Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC 27695, USA
    Biometals 18:449-66. 2005
    ..Depending on the type of divalent metal ion present, in vitro phosphorylation of Spo0F by its cognate kinase KinA can be inhibited...
  46. ncbi request reprint Phosphorylation and functional analysis of the sporulation initiation factor Spo0A from Clostridium botulinum
    Kristina Wörner
    The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Mol Microbiol 59:1000-12. 2006
    ..botulinum Spo0A, some of these interactions have been lost...
  47. pmc The crystal structure of YycH involved in the regulation of the essential YycFG two-component system in Bacillus subtilis reveals a novel tertiary structure
    Hendrik Szurmant
    Division of Cellular Biology, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    Protein Sci 15:929-34. 2006
    ..3 A data to an R-factor of 25.2%. The molecule is made up of three domains, and has a novel three-dimensional structure. The N-terminal domain features a calcium binding site and the central domain contains two conserved loop regions...
  48. pmc Sensor domains encoded in Bacillus anthracis virulence plasmids prevent sporulation by hijacking a sporulation sensor histidine kinase
    Andrea K White
    Division of Cellular Biology, Mail Code MEM 116, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Bacteriol 188:6354-60. 2006
    ..anthracis sporulation in the bloodstream of its infected host, a salient characteristic in the virulence of this organism, and provides an additional role for the virulence plasmids in anthrax pathogenesis...
  49. ncbi request reprint Features of protein-protein interactions in two-component signaling deduced from genomic libraries
    Robert A White
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, USA
    Methods Enzymol 422:75-101. 2007
    ....
  50. ncbi request reprint Virulence- and antibiotic resistance-associated two-component signal transduction systems of Gram-positive pathogenic bacteria as targets for antimicrobial therapy
    Keith Stephenson
    Division of Cellular Biology, Department of Molecular and Experimental Medicine, MEM 116, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Pharmacol Ther 93:293-305. 2002
    ..This review discusses the potential use of two-component systems as targets for antimicrobial therapy against Gram-positive pathogens and the current status in the development of inhibitors specific for these systems...
  51. pmc Co-evolving motions at protein-protein interfaces of two-component signaling systems identified by covariance analysis
    Hendrik Szurmant
    Division of Cellular Biology, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    Biochemistry 47:7782-4. 2008
    ..NMR dynamics and structural studies of both wild-type and mutant proteins in this cluster suggest that motions serve to precisely arrange the site of phosphoryl transfer within the complex...

Research Grants19

  1. Signal Transduction Networks in Bacillus anthracis
    JAMES ALFRED HOCH; Fiscal Year: 2010
    ..This program has the goal of generating knowledge of the pathogenic regulatory mechanisms employed by toxin producing spore-forming bacteria for which little is known. ..
  2. GENETIC CONTROL OF DEVELOPMENT
    JAMES HOCH; Fiscal Year: 2007
    ..The significance of this research is not limited to understanding the phosphorelay and sporulation in a corner of the bacterial world but has regulatory implications for signal transduction in eukaryotes and plants. ..
  3. Signal Transduction Networks in Bacillus anthracis
    JAMES HOCH; Fiscal Year: 2007
    ..This program has the goal of generating knowledge of the pathogenic regulatory mechanisms employed by toxin producing spore-forming bacteria for which little is known. ..
  4. Signal Transduction Networks in Bacillus anthracis
    JAMES HOCH; Fiscal Year: 2006
    ..This is a collaborative effort between investigators with expertise in genetics, biochemistry and structure of Bacillus regulatory proteins. ..
  5. GENETIC CONTROL OF DEVELOPMENT
    JAMES HOCH; Fiscal Year: 2005
    ..It is believed that this combination of structural and functional studies will lead to effective anti-bacterial agents either directly or in combination as inhibitors of resistance mechanisms. ..
  6. GENETIC CONTROL OF DEVELOPMENT
    JAMES HOCH; Fiscal Year: 1991
    ....
  7. GENETIC CONTROL OF DEVELOPMENT
    JAMES ALFRED HOCH; Fiscal Year: 2010
    ..The significance of this research is not limited to understanding the phosphorelay and sporulation in a corner of the bacterial world but has regulatory implications for signal transduction in eukaryotes and plants. ..