Russ Hille

Summary

Affiliation: The Ohio State University
Country: USA

Publications

  1. pmc Substrate Orientation and Catalysis at the Molybdenum Site in Xanthine Oxidase: CRYSTAL STRUCTURES IN COMPLEX WITH XANTHINE AND LUMAZINE
    James M Pauff
    Department of Biochemistry, University of California, Riverside, California 92521 and the Medical Scientist Program, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 284:8760-7. 2009
  2. ncbi request reprint Molybdenum-containing hydroxylases
    Russ Hille
    Department of Molecular and Cellular Biochemistry, The Ohio State University, 333 Hamilton Hall, 1645 Neil Avenue, Columbus, OH 43210, USA
    Arch Biochem Biophys 433:107-16. 2005
  3. ncbi request reprint Resonance Raman studies of xanthine oxidase: The reduced enzyme-product complex with violapterin
    Craig Hemann
    Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA
    J Phys Chem B 109:3023-31. 2005
  4. ncbi request reprint Spectroscopic and kinetic studies of Y114F and W116F mutants of Me2SO reductase from Rhodobacter capsulatus
    Nathan Cobb
    Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 282:35519-29. 2007
  5. ncbi request reprint The role of arginine 310 in catalysis and substrate specificity in xanthine dehydrogenase from Rhodobacter capsulatus
    James M Pauff
    Department of Molecular and Cellular Biochemistry, Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 282:12785-90. 2007
  6. ncbi request reprint Spectroscopic and kinetic studies of Arabidopsis thaliana sulfite oxidase: nature of the redox-active orbital and electronic structure contributions to catalysis
    Craig Hemann
    Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA
    J Am Chem Soc 127:16567-77. 2005
  7. ncbi request reprint Mechanistic studies of Rhodobacter sphaeroides Me2SO reductase
    Nathan Cobb
    Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, Ohio 43210 1218, USA
    J Biol Chem 280:11007-17. 2005
  8. pmc Oxidative half-reaction of arabidopsis thaliana sulfite oxidase: generation of superoxide by a peroxisomal enzyme
    Robert S Byrne
    Department of Molecular and Cellular Biochemistry, University of California, Riverside, California 92521, USA
    J Biol Chem 284:35479-84. 2009
  9. ncbi request reprint Studies on the mechanism of action of xanthine oxidase
    Eun Young Choi
    Department of Molecular and Cellular Biochemistry, The Ohio State University, 333 Hamilton Hall, 1645 Neil Avenue, Columbus, OH 43210 1218, USA
    J Inorg Biochem 98:841-8. 2004
  10. ncbi request reprint Molybdenum and tungsten in biology
    Russ Hille
    Dept of Molecular and Cellular Biochemistry and The Protein Research Group, The Ohio State University, Columbus, OH 43210 1218, USA
    Trends Biochem Sci 27:360-7. 2002

Collaborators

Detail Information

Publications33

  1. pmc Substrate Orientation and Catalysis at the Molybdenum Site in Xanthine Oxidase: CRYSTAL STRUCTURES IN COMPLEX WITH XANTHINE AND LUMAZINE
    James M Pauff
    Department of Biochemistry, University of California, Riverside, California 92521 and the Medical Scientist Program, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 284:8760-7. 2009
    ..The mechanistic implications as to how the ensemble of active site functional groups in the active site work to accelerate reaction rate are discussed...
  2. ncbi request reprint Molybdenum-containing hydroxylases
    Russ Hille
    Department of Molecular and Cellular Biochemistry, The Ohio State University, 333 Hamilton Hall, 1645 Neil Avenue, Columbus, OH 43210, USA
    Arch Biochem Biophys 433:107-16. 2005
    ..The present minireview summarizes recent mechanistic and structure/function studies of members of this large and growing family of enzymes...
  3. ncbi request reprint Resonance Raman studies of xanthine oxidase: The reduced enzyme-product complex with violapterin
    Craig Hemann
    Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA
    J Phys Chem B 109:3023-31. 2005
    ..Given the numerous modes involving the bridging oxygen, a side-on binding mode can be eliminated...
  4. ncbi request reprint Spectroscopic and kinetic studies of Y114F and W116F mutants of Me2SO reductase from Rhodobacter capsulatus
    Nathan Cobb
    Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 282:35519-29. 2007
    ..This "redox-cycled" mutant exhibits a Me(2)SO reducing activity and overall reaction mechanism similar to that of wild-type enzyme but rapidly reverts to the inactive five-coordinate form in the course of turnover...
  5. ncbi request reprint The role of arginine 310 in catalysis and substrate specificity in xanthine dehydrogenase from Rhodobacter capsulatus
    James M Pauff
    Department of Molecular and Cellular Biochemistry, Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 282:12785-90. 2007
    ....
  6. ncbi request reprint Spectroscopic and kinetic studies of Arabidopsis thaliana sulfite oxidase: nature of the redox-active orbital and electronic structure contributions to catalysis
    Craig Hemann
    Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA
    J Am Chem Soc 127:16567-77. 2005
    ..Electron occupancy of this Mo=O(eq) pi* redox orbital upon interaction with substrates would effectively labilize the Mo=O(eq) bond, providing the dominant contribution to lowering the activation energy for oxygen atom transfer...
  7. ncbi request reprint Mechanistic studies of Rhodobacter sphaeroides Me2SO reductase
    Nathan Cobb
    Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, Ohio 43210 1218, USA
    J Biol Chem 280:11007-17. 2005
    ....
  8. pmc Oxidative half-reaction of arabidopsis thaliana sulfite oxidase: generation of superoxide by a peroxisomal enzyme
    Robert S Byrne
    Department of Molecular and Cellular Biochemistry, University of California, Riverside, California 92521, USA
    J Biol Chem 284:35479-84. 2009
    ..The physiological implications of plant sulfite oxidase as a copious generator of superoxide are discussed...
  9. ncbi request reprint Studies on the mechanism of action of xanthine oxidase
    Eun Young Choi
    Department of Molecular and Cellular Biochemistry, The Ohio State University, 333 Hamilton Hall, 1645 Neil Avenue, Columbus, OH 43210 1218, USA
    J Inorg Biochem 98:841-8. 2004
    ..Preliminary experiments with two such mutants are entirely consistent with the proposed catalytic roles of two active site glutamate residues...
  10. ncbi request reprint Molybdenum and tungsten in biology
    Russ Hille
    Dept of Molecular and Cellular Biochemistry and The Protein Research Group, The Ohio State University, Columbus, OH 43210 1218, USA
    Trends Biochem Sci 27:360-7. 2002
    ..Together, these studies provide an increasingly detailed view of the reaction mechanisms and the correlation between the electronic structure of the active site and catalytic function, one of the fundamental goals in metallobiochemistry...
  11. pmc Reduced nonprotein thiols inhibit activation and function of MMP-9: implications for chemoprevention
    Ping Pei
    Department of Oral Maxillofacial Surgery and Pathology, College of Dentistry, The Ohio State University, Columbus, OH 43210, USA
    Free Radic Biol Med 41:1315-24. 2006
    ..Collectively, these data demonstrate that nonprotein thiols suppress MMP-9 activation and function and introduce the prospect for their use in chemopreventive applications...
  12. ncbi request reprint The interaction of trimethylamine dehydrogenase and electron-transferring flavoprotein
    Weiwei Shi
    Department of Molecular and Cellular Biochemistry, Ohio State University, Columbus, Ohio 43210 1218, USA
    J Biol Chem 280:20239-46. 2005
    ....
  13. ncbi request reprint Oxo, sulfido, and tellurido Mo-enedithiolate models for xanthine oxidase: understanding the basis of enzyme reactivity
    Predrag Ilich
    Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA
    J Am Chem Soc 124:6796-7. 2002
    ....
  14. ncbi request reprint The active site of arsenite oxidase from Alcaligenes faecalis
    Thomas Conrads
    Department of Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA
    J Am Chem Soc 124:11276-7. 2002
    ....
  15. pmc Ser170 of Bacillus thuringiensis Cry1Ab delta-endotoxin becomes anchored in a hydrophobic moiety upon insertion of this protein into Manduca sexta brush border membranes
    Oscar Alzate
    Biochemistry Department, The Ohio State University, Columbus, OH 43210, USA
    BMC Biochem 10:25. 2009
    ....
  16. ncbi request reprint Crystal structure and stability studies of C77S HiPIP: a serine ligated [4Fe-4S] cluster
    Sheref S Mansy
    Evans Laboratory of Chemistry, Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210, USA
    Biochemistry 41:1195-201. 2002
    ..Ser ligation ultimately results in decreased cluster stability due to increased sensitivity to proton mediated degradation...
  17. ncbi request reprint Characterization of superoxide production from aldehyde oxidase: an important source of oxidants in biological tissues
    Tapan Kumar Kundu
    Center for Biomedical EPR Spectroscopy and Imaging, The Davis Heart and Lung Research Institute, Division of Cardiovascular Medicine, Department of Internal Medicine, Ohio State University College of Medicine, Columbus, OH 43210, USA
    Arch Biochem Biophys 460:113-21. 2007
    ..In view of the ubiquitous distribution of aldehydes in tissues, aldehyde oxidase can be an important basal source of superoxide that would be enhanced in disease settings where cellular aldehyde levels are increased...
  18. ncbi request reprint Molybdenum enzymes containing the pyranopterin cofactor: an overview
    Russ Hille
    Department of Molecular and Cellular Biochemistry, Ohio State University, Columbus, OH 43210 1218, USA
    Met Ions Biol Syst 39:187-226. 2002
  19. ncbi request reprint The reaction mechanism of xanthine oxidase: evidence for two-electron chemistry rather than sequential one-electron steps
    Amy L Stockert
    Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA
    J Am Chem Soc 124:14554-5. 2002
    ..Our results provide additional support to current evidence for the favored two-electron reduction mechanism...
  20. ncbi request reprint Iron-sulfur cluster biosynthesis: characterization of Schizosaccharomyces pombe Isa1
    Gong Wu
    Evans Laboratory of Chemistry, Ohio State University, 100 West 18th Avenue, Columbus, OH 43210, USA
    J Biol Inorg Chem 7:526-32. 2002
    ..Electronic supplementary material to this paper, containing Mössbauer and UV-visible spectra for mutant Isa1 proteins, can be obtained by using the Springer Link server located at http://dx.doi.org/10.1007/s00775-001-0330-2...
  21. ncbi request reprint The role of active site glutamate residues in catalysis of Rhodobacter capsulatus xanthine dehydrogenase
    Silke Leimkuhler
    Department of Plant Biology, Technical University Braunschweig, 38023 Braunschweig, Germany
    J Biol Chem 279:40437-44. 2004
    ..This result is fully consistent with the proposed role of this residue as an active site base that initiates catalysis...
  22. ncbi request reprint Substrate orientation in xanthine oxidase: crystal structure of enzyme in reaction with 2-hydroxy-6-methylpurine
    James M Pauff
    Department of Biochemistry, University of California, Riverside, California 92521, USA
    J Biol Chem 283:4818-24. 2008
    ....
  23. pmc The crystal structure of xanthine oxidoreductase during catalysis: implications for reaction mechanism and enzyme inhibition
    Ken Okamoto
    Department of Biochemistry and Molecular Biology, Nippon Medical School, 1 1 5 Sendagi, Bunkyo ku, Tokyo 113 8602, Japan
    Proc Natl Acad Sci U S A 101:7931-6. 2004
    ..A water molecule usually seen in the active site of the enzyme is absent in the present structure, which probably accounts for the stability of this intermediate toward ligand displacement by hydroxide...
  24. ncbi request reprint Electrochemical studies of arsenite oxidase: an unusual example of a highly cooperative two-electron molybdenum center
    Kevin R Hoke
    Inorganic Chemistry Laboratory, Oxford University, South Parks Road, Oxford OX1 3QR, England
    Biochemistry 43:1667-74. 2004
    ..0 and 20 degrees C, that of the [3Fe-4S] center is approximately 260 mV and that of the Rieske center is approximately 130 mV...
  25. ncbi request reprint Structure of tetrahydrobiopterin tunes its electron transfer to the heme-dioxy intermediate in nitric oxide synthase
    Chin Chuan Wei
    Department of Immunology, The Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Biochemistry 42:1969-77. 2003
    ..4) Faster electron transfer from 5-methyl-H(4)B may be due to increased radical stability afforded by the N-5 methyl group...
  26. ncbi request reprint Structures of the Mo(V) forms of sulfite oxidase from Arabidopsis thaliana by pulsed EPR spectroscopy
    Andrei V Astashkin
    Department of Chemistry, University of Arizona, 1306 East University Boulevard, Tucson, Arizona 85721 0041, USA
    Biochemistry 44:13274-81. 2005
    ..At high pH, the active site is in the "open form", and water can readily exchange into the site to generate the hpH SO...
  27. ncbi request reprint Nature of the catalytically labile oxygen at the active site of xanthine oxidase
    Christian J Doonan
    Department of Geological Sciences, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada
    J Am Chem Soc 127:4518-22. 2005
    ..These results support a mechanism initiated by base-assisted nucleophilic attack of the substrate by Mo-OH...
  28. ncbi request reprint Plants have SOX: the structure of sulfite oxidase from Arabidopsis thaliana
    Russ Hille
    Structure 11:1189-90. 2003
  29. pmc Direct demonstration of the presence of coordinated sulfate in the reaction pathway of Arabidopsis thaliana sulfite oxidase using 33S labeling and ESEEM spectroscopy
    Andrei V Astashkin
    Department of Chemistry, University of Arizona, Tucson, Arizona 85721 0041, USA
    J Am Chem Soc 129:14800-10. 2007
    ..The isotropic hfi constant of 33S determined in these experiments was about 3 MHz, which demonstrates the presence of coordinated sulfate in the sulfite-reduced low-pH form of the plant enzyme...
  30. pmc Catalytic reduction of a tetrahydrobiopterin radical within nitric-oxide synthase
    Chin Chuan Wei
    Department of Pathobiology, The Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    J Biol Chem 283:11734-42. 2008
    ..These results, in light of existing NOS crystal structures, suggest a "through-heme" mechanism may operate for H(4)B radical reduction in NOS...
  31. pmc Differences in a conformational equilibrium distinguish catalysis by the endothelial and neuronal nitric-oxide synthase flavoproteins
    Robielyn P Ilagan
    Department of Pathobiology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    J Biol Chem 283:19603-15. 2008
    ..We conclude that the set point and regulation of the FMN conformational equilibrium differ markedly in eNOSr and nNOSr and can explain the lower electron transfer activity of eNOSr...
  32. ncbi request reprint Electron transfer within complex II. Succinate:ubiquinone oxidoreductase of Escherichia coli
    Robert F Anderson
    Department of Chemistry, The University of Auckland, Auckland 1, New Zealand
    J Biol Chem 280:33331-7. 2005
    ....
  33. ncbi request reprint A tetrahydrobiopterin radical forms and then becomes reduced during Nomega-hydroxyarginine oxidation by nitric-oxide synthase
    Chin Chuan Wei
    Department of Immunology, The Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    J Biol Chem 278:46668-73. 2003
    ..These redox roles are novel and expand our understanding of biopterin function in biology...