M Herlyn

Summary

Affiliation: The Wistar Institute
Country: USA

Publications

  1. pmc Human breast cancer associated fibroblasts exhibit subtype specific gene expression profiles
    Julia Tchou
    Department of Surgery, Division of Endocrine and Oncologic Surgery, Rena Rowan Breast Center, Abramson Cancer Center, Perelman School of Medicine of the University of Pennsylvania, Philadelphia, PA 19104, USA
    BMC Med Genomics 5:39. 2012
  2. pmc Matricellular proteins produced by melanocytes and melanomas: in search for functions
    Mizuho Fukunaga Kalabis
    Molecular and Cellular Oncogenesis Program, The Wistar Institute, 3601 Spruce Street, Philadelphia, PA, 19104, USA
    Cancer Microenviron 1:93-102. 2008
  3. doi request reprint Driving in the melanoma landscape
    Meenhard Herlyn
    The Wistar Institute, Philadelphia, PA 19104, USA
    Exp Dermatol 18:506-8. 2009
  4. ncbi request reprint Roadmap for new opportunities in melanoma research
    Meenhard Herlyn
    Molecular and Cellular Oncogenesis, The Wistar Institute, Philadelphia, PA 19104, USA
    Semin Oncol 34:566-76. 2007
  5. doi request reprint Melanocytes: from morphology to application
    A Santiago-Walker
    Molecular and Cellular Oncogenesis Program, The Wistar Institute, Philadelphia, PA 19104, USA
    Skin Pharmacol Physiol 22:114-21. 2009
  6. ncbi request reprint Farming cells to rebuild skin and melanoma
    Meenhard Herlyn
    The Wistar Institute, Philadelphia, Pennsylvannia 19104, USA
    Cancer Biol Ther 6:467-71. 2007
  7. ncbi request reprint Molecular targets in melanoma: strategies and challenges for diagnosis and therapy
    Meenhard Herlyn
    The Wistar Institute, Philadelphia, PA, USA
    Int J Cancer 118:523-6. 2006
  8. pmc Basic fibroblast growth factor and ultraviolet B transform melanocytes in human skin
    C Berking
    The Wistar Institute, 3601 Spruce St, Philadelphia, PA 19104, USA
    Am J Pathol 158:943-53. 2001
  9. ncbi request reprint Downregulation of CCN3 expression as a potential mechanism for melanoma progression
    M Fukunaga-Kalabis
    Molecular and Cellular Oncogenesis Program, The Wistar Institute, Philadelphia, PA 19104, USA
    Oncogene 27:2552-60. 2008
  10. ncbi request reprint N-cadherin-mediated intercellular interactions promote survival and migration of melanoma cells
    G Li
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Cancer Res 61:3819-25. 2001

Research Grants

  1. Second International Melanoma Research Congress
    Meenhard Herlyn; Fiscal Year: 2004
  2. Biology of Melanoma Matastasis
    Meenhard Herlyn; Fiscal Year: 2007
  3. Cell-Cell Communication During Melanoma Development
    Meenhard Herlyn; Fiscal Year: 2007
  4. EXPERIMENTAL TRANSFORMATION OF HUMAN MELANOCYTES IN VIVO
    Meenhard Herlyn; Fiscal Year: 2007
  5. SPORE in Skin Cancer
    Meenhard Herlyn; Fiscal Year: 2007
  6. Cell-Cell Communication During Melanoma Development
    Meenhard Herlyn; Fiscal Year: 2009
  7. CELL/CELL COMMUNICATION DURING MELANOMA PROGRESSION
    Meenhard Herlyn; Fiscal Year: 2000
  8. METASTASIS--BIOLOGICAL PHENOTYPE AND MODELS FOR THERAPY
    Meenhard Herlyn; Fiscal Year: 2002
  9. EXPERIMENTAL TRANSFORMATION OF HUMAN MELANOCYTES IN VIVO
    Meenhard Herlyn; Fiscal Year: 2003
  10. CELL CELL COMMUNICATION DURING MELANOMA DEVELOPMENT
    Meenhard Herlyn; Fiscal Year: 2004

Collaborators

Detail Information

Publications113 found, 100 shown here

  1. pmc Human breast cancer associated fibroblasts exhibit subtype specific gene expression profiles
    Julia Tchou
    Department of Surgery, Division of Endocrine and Oncologic Surgery, Rena Rowan Breast Center, Abramson Cancer Center, Perelman School of Medicine of the University of Pennsylvania, Philadelphia, PA 19104, USA
    BMC Med Genomics 5:39. 2012
    ..Several studies have reported gene expression profile differences between CAFs and normal breast fibroblasts but in none of these studies were the results stratified based on tumor subtypes...
  2. pmc Matricellular proteins produced by melanocytes and melanomas: in search for functions
    Mizuho Fukunaga Kalabis
    Molecular and Cellular Oncogenesis Program, The Wistar Institute, 3601 Spruce Street, Philadelphia, PA, 19104, USA
    Cancer Microenviron 1:93-102. 2008
    ..Qualitative and quantitative changes in matricellular protein expression contribute to melanoma progression similar to the E-cadherin to N-cadherin class switch, allowing melanoma cells to escape from keratinocyte control...
  3. doi request reprint Driving in the melanoma landscape
    Meenhard Herlyn
    The Wistar Institute, Philadelphia, PA 19104, USA
    Exp Dermatol 18:506-8. 2009
    ..Melanomas as a group are heterogeneous as are tumor cells within one lesion. New strategies will move towards individualized therapies and combination therapies to target all cells within a tumor...
  4. ncbi request reprint Roadmap for new opportunities in melanoma research
    Meenhard Herlyn
    Molecular and Cellular Oncogenesis, The Wistar Institute, Philadelphia, PA 19104, USA
    Semin Oncol 34:566-76. 2007
    ..This group provides recommendations for both short- and long-term strategies that build on research strengths and opportunities established by the many members of the research community...
  5. doi request reprint Melanocytes: from morphology to application
    A Santiago-Walker
    Molecular and Cellular Oncogenesis Program, The Wistar Institute, Philadelphia, PA 19104, USA
    Skin Pharmacol Physiol 22:114-21. 2009
    ..Here we present the biological basis for the use of organotypic, three-dimensional model systems in the study of melanoma, and highlight the features of the most utilized organotypic model systems...
  6. ncbi request reprint Farming cells to rebuild skin and melanoma
    Meenhard Herlyn
    The Wistar Institute, Philadelphia, Pennsylvannia 19104, USA
    Cancer Biol Ther 6:467-71. 2007
  7. ncbi request reprint Molecular targets in melanoma: strategies and challenges for diagnosis and therapy
    Meenhard Herlyn
    The Wistar Institute, Philadelphia, PA, USA
    Int J Cancer 118:523-6. 2006
    ..However, major challenges lie ahead in securing funding, building infrastructure and gaining expertise in new technologies. To meet these challenges, multidisciplinary collaborations will be required all the more...
  8. pmc Basic fibroblast growth factor and ultraviolet B transform melanocytes in human skin
    C Berking
    The Wistar Institute, 3601 Spruce St, Philadelphia, PA 19104, USA
    Am J Pathol 158:943-53. 2001
    ..This is the first report suggesting that an imbalance of physiological growth factor production in the skin may cause melanoma in combination with UVB...
  9. ncbi request reprint Downregulation of CCN3 expression as a potential mechanism for melanoma progression
    M Fukunaga-Kalabis
    Molecular and Cellular Oncogenesis Program, The Wistar Institute, Philadelphia, PA 19104, USA
    Oncogene 27:2552-60. 2008
    ..Whereas major matricellular proteins, such as osteopontin, tenascin or secreted protein acidic and rich in cysteine (SPARC), are strongly upregulated in melanoma cells; CCN3 is the first member of this family that is downregulated...
  10. ncbi request reprint N-cadherin-mediated intercellular interactions promote survival and migration of melanoma cells
    G Li
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Cancer Res 61:3819-25. 2001
    ....
  11. ncbi request reprint Mel-CAM-specific genetic suppressor elements inhibit melanoma growth and invasion through loss of gap junctional communication
    K Satyamoorthy
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania, PA 19104 USA
    Oncogene 20:4676-84. 2001
    ..These results suggest the multifunctional role of a melanoma-associated cell-cell adhesion receptor in tumor progression...
  12. ncbi request reprint Lessons from melanocyte development for understanding the biological events in naevus and melanoma formation
    M Herlyn
    The Wistar Institute, Philadelphia, PA 19104, USA
    Melanoma Res 10:303-12. 2000
    ..Even highly aggressive metastatic melanoma cells can be signalled to turn off the expression of genes associated with tumour invasion and metastasis, suggesting that this strategy could be utilized in the therapy of melanoma...
  13. ncbi request reprint Prolonged response to antisense cyclin D1 in a human squamous cancer xenograft model
    E R Sauter
    Department of Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Clin Cancer Res 6:654-60. 2000
    ..Cyclin D1 expression increases in frequency with disease progression, and antisense cyclin D1 was effective in a xenograft model of human cancer, independent of tumor growth rate...
  14. ncbi request reprint Transforming growth factor-beta1 increases survival of human melanoma through stroma remodeling
    C Berking
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Cancer Res 61:8306-16. 2001
    ..These data suggest that remodeling of the neighboring stroma, which provides a supporting scaffolding and a positive feedback stimulation of tumor growth, is an important function of TGF-beta1 in melanoma...
  15. pmc CRAF inhibition induces apoptosis in melanoma cells with non-V600E BRAF mutations
    K S M Smalley
    The Wistar Institute, Philadelphia, PA, USA
    Oncogene 28:85-94. 2009
    ..In summary, we have identified a group of melanomas with low-activity BRAF mutations that are reliant upon CRAF-mediated survival activity...
  16. ncbi request reprint Low-level monocyte chemoattractant protein-1 stimulation of monocytes leads to tumor formation in nontumorigenic melanoma cells
    M Nesbit
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    J Immunol 166:6483-90. 2001
    ..This correlates with the degree of monocytic cell infiltration, which results in increased tumor vascularization and TNF-alpha production...
  17. ncbi request reprint Insulin-like growth factor-1 induces survival and growth of biologically early melanoma cells through both the mitogen-activated protein kinase and beta-catenin pathways
    K Satyamoorthy
    The Wistar Institute, Biomedical Graduate Studies, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Cancer Res 61:7318-24. 2001
    ....
  18. ncbi request reprint Dynamics of cell interactions and communications during melanoma development
    G Li
    The Wistar Institute, Philadelphia, PA 19104, USA
    Crit Rev Oral Biol Med 13:62-70. 2002
    ..Thus, abnormal expression of intercellular adhesion receptors and dysregulated intercellular communication underlies melanoma development and progression...
  19. ncbi request reprint Role of cadherins and matrixins in melanoma
    C Gruss
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Curr Opin Oncol 13:117-23. 2001
    ..Advances in these fields will lead to the development of better tools for prevention, diagnosis, and therapy...
  20. pmc Evidence for mesenchymal-like sub-populations within squamous cell carcinomas possessing chemoresistance and phenotypic plasticity
    D Basu
    Department of Otorhinolaryngology Head and Neck Surgery, University of Pennsylvania, Philadelphia, PA 19104, USA
    Oncogene 29:4170-82. 2010
    ..Taken together, these results provide evidence for a low-turnover, mesenchymal-like sub-population in SCCs with diminished EGFR pathway function and intrinsic resistance to conventional and EGFR-targeted chemotherapies...
  21. ncbi request reprint Microarray analysis of phosphatase gene expression in human melanoma
    L McArdle
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Br J Dermatol 152:925-30. 2005
    ..However, elevation may also arise due to decreased protein tyrosine phosphatase (PTP) expression...
  22. pmc Ki67 expression levels are a better marker of reduced melanoma growth following MEK inhibitor treatment than phospho-ERK levels
    K S M Smalley
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania, 19104, USA
    Br J Cancer 96:445-9. 2007
    ..Here, we demonstrate that there is a poor correlation between pERK inhibition and the anti-proliferative effects of MEK inhibitors in melanoma cells. We suggest that Ki67 is a better biomarker for future clinical studies...
  23. ncbi request reprint A versatile method for the removal of melanin from ribonucleic acids in melanocytic cells
    K Satyamoorthy
    The Wistar Institute, Philadelphia, PA 19104, USA
    Melanoma Res 12:449-52. 2002
    ..Although the process results in some loss of input RNA, this purification procedure is simple, robust and can easily be adopted in any laboratory for the molecular analysis of RNA that requires removal of melanin contamination...
  24. pmc Structure-based design of an organoruthenium phosphatidyl-inositol-3-kinase inhibitor reveals a switch governing lipid kinase potency and selectivity
    Peng Xie
    Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    ACS Chem Biol 3:305-16. 2008
    ....
  25. ncbi request reprint Notch1 signaling promotes primary melanoma progression by activating mitogen-activated protein kinase/phosphatidylinositol 3-kinase-Akt pathways and up-regulating N-cadherin expression
    Zhao Jun Liu
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Cancer Res 66:4182-90. 2006
    ..Our data show regulation of MAPK/PI3K-Akt pathway activities and expression of N-cadherin by the Notch pathway and provide a mechanistic basis for Notch signaling in the promotion of primary melanoma progression...
  26. ncbi request reprint Osteonectin/SPARC induction by ectopic beta(3) integrin in human radial growth phase primary melanoma cells
    Richard A Sturm
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Cancer Res 62:226-32. 2002
    ....
  27. pmc Tenascin-C promotes melanoma progression by maintaining the ABCB5-positive side population
    M Fukunaga-Kalabis
    Molecular and Cellular Oncogenesis Program, Division of Molecular and Cellular Biology, The Wistar Institute, Philadelphia, PA 19104, USA
    Oncogene 29:6115-24. 2010
    ..These data suggest that TNC is critical in melanoma progression as it mediates protective signals in the therapy-resistant population of melanoma...
  28. ncbi request reprint Human skin reconstruct models: a new application for studies of melanocyte and melanoma biology
    C Berking
    The Wistar Institute, Philadelphia, PA 19104, USA
    Histol Histopathol 16:669-74. 2001
    ..In this review, principles and different methods of skin reconstruction are introduced with focus on the application for pigment cell biology...
  29. pmc Up-regulated expression of zonula occludens protein-1 in human melanoma associates with N-cadherin and contributes to invasion and adhesion
    Keiran S M Smalley
    Wistar Institute, 3601 Spruce St, Philadelphia, Pennsylvania 19104, USA
    Am J Pathol 166:1541-54. 2005
    ....
  30. pmc Distinct patterns of DNA copy number alterations associate with BRAF mutations in melanomas and melanoma-derived cell lines
    J Greshock
    Translational Medicine Oncology, GlaxoSmithKline, King of Prussia, PA, USA
    Genes Chromosomes Cancer 48:419-28. 2009
    ..The genetic loci that make up this profile may harbor therapeutic targets specific for tumors with BRAF mutations...
  31. ncbi request reprint Fibroblast growth factor-binding protein expression changes with disease progression in clinical and experimental human squamous epithelium
    E R Sauter
    The Wistar Institute, Philadelphia, PA, USA
    Int J Cancer 92:374-81. 2001
    ..Taken together, these data suggest that FGF-BP expression in squamous epithelium does not play an important role in progression to invasive carcinoma...
  32. ncbi request reprint Downregulation of E-cadherin and Desmoglein 1 by autocrine hepatocyte growth factor during melanoma development
    G Li
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania, PA 19104, USA
    Oncogene 20:8125-35. 2001
    ....
  33. ncbi request reprint No longer a molecular black box--new clues to apoptosis and drug resistance in melanoma
    K Satyamoorthy
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Trends Mol Med 7:191-4. 2001
    ..Hence, the genes and proteins that control apoptosis provide exciting new targets for rationally designed anti-melanoma therapeutic strategies...
  34. doi request reprint Learning the ABCs of melanoma-initiating cells
    Susan E Zabierowski
    Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Cancer Cell 13:185-7. 2008
    ..The identification of melanoma-initiating cells has far-reaching implications, as new therapeutic strategies can be envisioned that specifically target these cells...
  35. ncbi request reprint Reversal of melanocytic malignancy by keratinocytes is an E-cadherin-mediated process overriding beta-catenin signaling
    Gang Li
    The Wistar Institute, Philadelphia, PA 19104, USA
    Exp Cell Res 297:142-51. 2004
    ..The results indicate that E-cadherin-mediated cell adhesion is required for keratinocyte-mediated control of melanocytic cells, which can override proliferative activity of beta-catenin...
  36. ncbi request reprint Adhesion, migration and communication in melanocytes and melanoma
    Nikolas K Haass
    The Wistar Institute, Philadelphia, PA 19104, USA
    Pigment Cell Res 18:150-9. 2005
    ....
  37. ncbi request reprint The vascular phenotype of melanoma metastasis
    Omaida C Velazquez
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Clin Exp Metastasis 20:229-35. 2003
    ..Thus pathways for angiogenesis and vasculogenesis are guided through the cooperation of fibroblasts and melanoma cells perpetuated by the dominance of the metastatic melanoma cells...
  38. ncbi request reprint Reciprocal regulation of MelCAM and AKT in human melanoma
    Gang Li
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Oncogene 22:6891-9. 2003
    ..These data link AKT activation with MelCAM expression, and implicate that intervention of MelCAM-AKT signaling axis in melanoma is a potential therapeutical approach...
  39. ncbi request reprint Normal human melanocyte homeostasis as a paradigm for understanding melanoma
    Nikolas K Haass
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    J Investig Dermatol Symp Proc 10:153-63. 2005
    ....
  40. pmc Dysregulation of claudin-7 leads to loss of E-cadherin expression and the increased invasion of esophageal squamous cell carcinoma cells
    Mercedes Lioni
    The Wistar Institute, 3601 Spruce St, Philadelphia, PA 19104, USA
    Am J Pathol 170:709-21. 2007
    ..We also demonstrate a critical role for claudin-7 expression in the regulation of E-cadherin in these cells, suggesting this may be one mechanism for the loss of epithelial architecture and invasion observed in esophageal SCC...
  41. ncbi request reprint Characterization of monoclonal antibodies directed to the amino-terminus of the WT1, Wilms' tumor suppressor protein
    F J Rauscher
    The Wistar Institute, Philadelphia, PA 19104, USA
    Hybridoma 17:191-8. 1998
    ..These WT1-specific MAbs should be useful in characterizing the biochemical and developmental roles of WT1 and in defining the emerging role of WT1 as a diagnostic and/or prognostic marker in mesothelioma, leukemias, and breast cancer...
  42. ncbi request reprint Towards the targeted therapy of melanoma
    K S M Smalley
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Mini Rev Med Chem 6:387-93. 2006
    ..The recent years have seen great advances in understanding the biology of melanoma. In the current review we discuss the most promising molecular targets for melanoma and suggest possible strategies for overcoming resistance...
  43. ncbi request reprint Alpha5 and alpha2 integrin gene transfers mimic the PDGF-B-induced transformed phenotype of fibroblasts in human skin
    M Nesbit
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Lab Invest 81:1263-74. 2001
    ..A similar stromal response was induced when alpha5 and alpha2 integrin subunits were overexpressed in the human dermis, suggesting that integrins play a major role in the induction of a transformed phenotype of fibroblasts by PDGF-B...
  44. ncbi request reprint Emerging concepts and technologies in melanoma research
    M Herlyn
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Melanoma Res 12:3-8. 2002
    ..The field has to better bridge experimental with clinical research and increase communication. Corroboration with advocacy groups should activate the public for increased awareness and funding...
  45. ncbi request reprint The role of altered cell-cell communication in melanoma progression
    Nikolas K Haass
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    J Mol Histol 35:309-18. 2004
    ....
  46. ncbi request reprint Melanoma and the tumor microenvironment
    Jessie Villanueva
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Curr Oncol Rep 10:439-46. 2008
    ..It also discusses the influence of the microenvironment on therapeutic targeting of melanoma, highlighting recent studies that propose novel strategies to target tumor-microenvironment interactions...
  47. ncbi request reprint Targeting the stromal fibroblasts: a novel approach to melanoma therapy
    Keiran S M Smalley
    The Wistar Institute, Philadelphia, PA 19104, USA
    Expert Rev Anticancer Ther 5:1069-78. 2005
    ..More recent thinking posits that, although cancers are initiated through genetic mutation, progression is often the result of dynamic interactions between the tumor cells and their surrounding environment...
  48. doi request reprint Melanoma stem cells: the dark seed of melanoma
    Susan E Zabierowski
    The Wistar Institute, 3601 Spruce St, Philadelphia, PA 19104, USA
    J Clin Oncol 26:2890-4. 2008
    ..Ongoing studies are dissecting and characterizing the hierarchy of these subpopulations within a malignant lesion. Understanding these and the dynamics of clonal dominance will aid in the development of novel therapeutic strategies...
  49. ncbi request reprint Function and regulation of melanoma-stromal fibroblast interactions: when seeds meet soil
    Gang Li
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Oncogene 22:3162-71. 2003
    ..An understanding of this process and developing new experimental and screening models are of great importance for the development of effective therapeutical strategies to treat melanoma...
  50. ncbi request reprint Multiple signaling pathways must be targeted to overcome drug resistance in cell lines derived from melanoma metastases
    Keiran S M Smalley
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Mol Cancer Ther 5:1136-44. 2006
    ..It is further suggested that BRAF mutational status is not predictive of response to MEK inhibition under three-dimensional culture conditions...
  51. ncbi request reprint Differential response of primary and metastatic melanomas to neutrophils attracted by IL-8
    Helmut Schaider
    The Wistar Institute, Philadelphia, PA 19104, USA
    Int J Cancer 103:335-43. 2003
    ..Metastatic melanomas proliferate in vivo independently of infiltrating neutrophils...
  52. ncbi request reprint Unraveling mysteries of the multifunctional protein SPARC
    Mizuho Fukunaga-Kalabis
    Molecular and Cellular Oncogenesis Program, The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    J Invest Dermatol 127:2497-8. 2007
    ..In this issue, Prada et al. (2007) begin to answer this question by demonstrating that SPARC produced by melanoma, but not stromal cells, is essential to regulate melanoma cell growth...
  53. ncbi request reprint An organometallic protein kinase inhibitor pharmacologically activates p53 and induces apoptosis in human melanoma cells
    Keiran S M Smalley
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Cancer Res 67:209-17. 2007
    ..Taken together, our data provide a new strategy for the pharmacologic activation of p53 in melanoma, which may be a viable approach for overcoming apoptotic resistance in melanoma and offer new hope for rational melanoma therapy...
  54. ncbi request reprint Microenvironmental influences in melanoma progression
    John T Lee
    The Wistar Institute, Program of Molecular and Cellular Oncogenesis, Philadelphia, PA 19104, USA
    J Cell Biochem 101:862-72. 2007
    ..These promising model systems and their potential for closing current gaps in knowledge of disease are reviewed. The development of such models holds translational value that cannot be achieved with most current systems...
  55. pmc Bortezomib induces apoptosis in esophageal squamous cell carcinoma cells through activation of the p38 mitogen-activated protein kinase pathway
    Mercedes Lioni
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Mol Cancer Ther 7:2866-75. 2008
    ..We therefore suggest that p38 MAPK pathway activation is an excellent potential therapeutic strategy in ESCC. It is further suggested that bortezomib could be added to existing ESCC therapeutic regimens...
  56. pmc Coordinated functions of E-cadherin and transforming growth factor beta receptor II in vitro and in vivo
    Claudia D Andl
    Division of Gastroenterology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Cancer Res 66:9878-85. 2006
    ..Our results, which suggest that E-cadherin regulates TbetaRII function, have important implications for epithelial carcinogenesis characterized through the frequent occurrence of E-cadherin and TbetaRII loss...
  57. ncbi request reprint A tumorigenic subpopulation with stem cell properties in melanomas
    Dong Fang
    Program of Molecular and Cellular Oncogenesis, The Wistar Institute and Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, PA 19104, USA
    Cancer Res 65:9328-37. 2005
    ..Based on these findings, we propose that melanomas can contain a subpopulation of stem cells that contribute to heterogeneity and tumorigenesis. Targeting this population may lead to effective treatments for melanomas...
  58. pmc The functional interplay between EGFR overexpression, hTERT activation, and p53 mutation in esophageal epithelial cells with activation of stromal fibroblasts induces tumor development, invasion, and differentiation
    Takaomi Okawa
    Division of Gastroenterology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Genes Dev 21:2788-803. 2007
    ....
  59. pmc Human dermal stem cells differentiate into functional epidermal melanocytes
    Ling Li
    Molecular and Cellular Oncogenesis Program, The Wistar Institute, Philadelphia, PA 19104, USA
    J Cell Sci 123:853-60. 2010
    ....
  60. ncbi request reprint Life isn't flat: taking cancer biology to the next dimension
    Keiran S M Smalley
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    In Vitro Cell Dev Biol Anim 42:242-7. 2006
    ..It is likely that the adoption of these and other 3D models will allow us to more closely re-create the behavior of the tumor in vivo which may lead to identifying better anticancer drug candidates at an earlier stage of development...
  61. ncbi request reprint Melanoma-stroma interactions: structural and functional aspects
    Dirk Ruiter
    The Wistar Institute, Philadelphia, PA, USA
    Lancet Oncol 3:35-43. 2002
    ....
  62. pmc Active Notch1 confers a transformed phenotype to primary human melanocytes
    Chelsea C Pinnix
    Wistar Institute, Philadelphia, PA 19104, USA
    Cancer Res 69:5312-20. 2009
    ..This new information yields valuable insight into the basic epidemiology of melanoma and launches a realm of possibilities for drug intervention in this deadly disease...
  63. pmc In vivo and ex vivo MR imaging of slowly cycling melanoma cells
    S Magnitsky
    Laboratory of Molecular Imaging, Department of Radiology, Philadelphia, Pennsylvania, USA
    Magn Reson Med 66:1362-73. 2011
    ..Magnetic resonance imaging detects not only iron retaining melanoma cells but also iron positive macrophages. Proposed method opens up opportunities to image subpopulation of melanoma cells, which is critical for continuous tumor growth...
  64. pmc Oncogenic BRAF regulates beta-Trcp expression and NF-kappaB activity in human melanoma cells
    J Liu
    Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104 6046, USA
    Oncogene 26:1954-8. 2007
    ..Taken together, these data support a model in which mutational activation of BRAF in human melanomas contributes to constitutive induction of NF-kappaB activity and to increased survival of melanoma cells...
  65. ncbi request reprint Nuclear redistribution of BRCA1 during viral infection
    G G Maul
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Cell Growth Differ 9:743-55. 1998
    ..In contrast, infection with adenovirus 5 recruited BRCA1 to regions of viral transcription and replication. These disparate distributions of BRCA1 may provide clues to its function...
  66. ncbi request reprint Clinical protocol: Phase I trial to evaluate the safety of H5.020CMV.PDGF-B for the treatment of a diabetic insensate foot ulcer
    D J Margolis
    Department of Dermatology, Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Wound Repair Regen 8:480-93. 2000
    ..020CMV.PDGF-b associated with in vivo platelet-derived growth factor-BB gene transduction via an intraulcer injection of H5.020CMV.PDGF-b in patients with a diabetic insensate foot ulcer...
  67. pmc A temporarily distinct subpopulation of slow-cycling melanoma cells is required for continuous tumor growth
    Alexander Roesch
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Cell 141:583-94. 2010
    ..These results suggest a new understanding of melanoma heterogeneity with tumor maintenance as a dynamic process mediated by a temporarily distinct subpopulation...
  68. pmc New approaches to the biology of melanoma: a workshop of the National Institutes of Health Pathology B Study Section
    Meenhard Herlyn
    Wistar Institute, Philadelphia, Pennsylvania, USA
    Am J Pathol 161:1949-57. 2002
  69. pmc Nuclear cyclin D1/CDK4 kinase regulates CUL4 expression and triggers neoplastic growth via activation of the PRMT5 methyltransferase
    Priya Aggarwal
    Abramson Family Cancer Research Institute, Department of Cancer Biology, Abramson Cancer Center, University of Pennsylvania, Philadelphia, 19104, USA
    Cancer Cell 18:329-40. 2010
    ..Importantly, human cancers harboring mutations in Fbx4, the cyclin D1 E3 ligase, exhibit nuclear cyclin D1 accumulation and increased PRMT5 activity...
  70. pmc The essential role of fibroblasts in esophageal squamous cell carcinoma-induced angiogenesis
    Kazuhiro Noma
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Gastroenterology 134:1981-93. 2008
    ..Esophageal squamous cell carcinoma (ESCC) is known to be a highly angiogenic tumor. Here, we investigated the role of the stromal fibroblasts in the ESCC-induced angiogenic response using a novel 3-dimensional model...
  71. ncbi request reprint Mutant V600E BRAF increases hypoxia inducible factor-1alpha expression in melanoma
    Suresh M Kumar
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine and The Wistar Institute, Philadelphia, Pennsylvania, USA
    Cancer Res 67:3177-84. 2007
    ....
  72. doi request reprint Unraveling the mysteries of IGF-1 signaling in melanoma
    John T Lee
    Department of Molecular and Cellular Oncogenesis, The Wistar Institute, Philadelphia, PA 19104, USA
    J Invest Dermatol 128:1358-60. 2008
    ..The impact of this event is dramatic on several levels; avoidance of apoptosis not only prevents programmed cell death in an array of cell types but also promotes chemotherapeutic resistance during anticancer regimens...
  73. pmc Identification of a novel subgroup of melanomas with KIT/cyclin-dependent kinase-4 overexpression
    Keiran S M Smalley
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Cancer Res 68:5743-52. 2008
    ..This group of melanomas may be a subpopulation for which imatinib or other KIT inhibitors may constitute optimal therapy...
  74. pmc CCN3 controls 3D spatial localization of melanocytes in the human skin through DDR1
    Mizuho Fukunaga-Kalabis
    Molecular and Cellular Oncogenesis Program and 2Immunology Program, The Wistar Institute, Philadelphia, PA 19104, USA
    J Cell Biol 175:563-9. 2006
    ..These results demonstrate an intricate and necessary communication between keratinocytes and melanocytes in maintaining normal epidermal homeostasis...
  75. pmc Activation of Notch1 signaling is required for beta-catenin-mediated human primary melanoma progression
    Klara Balint
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    J Clin Invest 115:3166-76. 2005
    ..Inhibiting beta-catenin expression reversed Notch1-enhanced tumor growth and metastasis. Our data therefore suggest a beta-catenin-dependent, stage-specific role for Notch1 signaling in promoting the progression of primary melanoma...
  76. pmc Functional erythropoietin autocrine loop in melanoma
    Suresh M Kumar
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, 3400 Spruce Street, Philadelphia, PA 19104, USA
    Am J Pathol 166:823-30. 2005
    ..The results suggest that the autocrine and paracrine functions of Epo might play a role in malignant transformation of melanocytes and in the survival of melanoma cells in hypoxia and other adverse conditions...
  77. doi request reprint The mitogen-activated protein/extracellular signal-regulated kinase kinase inhibitor AZD6244 (ARRY-142886) induces growth arrest in melanoma cells and tumor regression when combined with docetaxel
    Nikolas K Haass
    The Wistar Institute, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Clin Cancer Res 14:230-9. 2008
    ..This study addresses the anti-melanoma activity of the MEK inhibitor AZD6244 (ARRY-142886)...
  78. pmc Overexpression of IL-8 in the cornea induces ulcer formation in the SCID mouse
    M Oka
    The Wistar Institute, Philadelphia, PA 19104, USA
    Br J Ophthalmol 90:612-5. 2006
    ..Although interleukin 8 (IL-8) is not produced in the normal cornea, it has been detected there in several pathological conditions. In this study, the direct effects of IL-8 overexpression on the cornea was examined...
  79. pmc AKT induces senescence in primary esophageal epithelial cells but is permissive for differentiation as revealed in organotypic culture
    K Oyama
    Gastroenterology Division and Department of Medicine, University of Pennsylvania, Philadelphia, PA 19085, USA
    Oncogene 26:2353-64. 2007
    ..In organotypic culture, AKT mediates changes related to cell shape and size with an expansion of the differentiated compartment...
  80. pmc Absence of BRAF mutations in UV-protected mucosal melanomas
    R H Edwards
    Abramson Family Cancer Research Institute, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Med Genet 41:270-2. 2004
    ..Thus, we determined the BRAF mutation frequency in a panel of 13 mucosal melanomas and compared those data with data from all currently published series of cutaneous melanomas...
  81. ncbi request reprint Constitutive mitogen-activated protein kinase activation in melanoma is mediated by both BRAF mutations and autocrine growth factor stimulation
    Kapaettu Satyamoorthy
    Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Cancer Res 63:756-9. 2003
    ..These data suggest that melanoma growth, invasion, and metastasis are attributable to constitutively activated ERK apparently mediated by excessive growth factors through autocrine mechanisms and BRAF kinase activation...
  82. ncbi request reprint Targeting intracellular signaling pathways as a novel strategy in melanoma therapeutics
    Keiran S M Smalley
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Ann N Y Acad Sci 1059:16-25. 2005
    ..In the current review, we discuss the role for these signaling pathways in melanoma and discuss the rationale for targeting signaling cascades using small molecule inhibitors...
  83. ncbi request reprint Induction of melanoma phenotypes in human skin by growth factors and ultraviolet B
    Carola Berking
    The Wistar Institute, Philadelphia, Pennsylvania, USA
    Cancer Res 64:807-11. 2004
    ..This is the first report on human cancer initiation in vivo in which an imbalance of physiological factors combined with an environmental carcinogen can lead to transformation of normal tissue...
  84. pmc Increased cyclin D1 expression can mediate BRAF inhibitor resistance in BRAF V600E-mutated melanomas
    Keiran S M Smalley
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Mol Cancer Ther 7:2876-83. 2008
    ....
  85. pmc A subpopulation of mouse esophageal basal cells has properties of stem cells with the capacity for self-renewal and lineage specification
    Jiri Kalabis
    Division of Gastroenterology, Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Clin Invest 118:3860-9. 2008
    ..These studies therefore provide a basis for understanding the regenerative capacity and biology of the esophageal epithelium when it is faced with injurious insults...
  86. ncbi request reprint Metastatic melanoma cells. Introduction
    Meenhard Herlyn
    The Wistar Institute, Philadelphia, PA, 19104, USA
    Cancer Metastasis Rev 24:193-4. 2005
  87. ncbi request reprint Photocarcinogenesis in human adult skin grafts
    Carola Berking
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Carcinogenesis 23:181-7. 2002
    ..The data suggest that melanocytes from young individuals are more susceptible to the transforming effects of genotoxic agents than melanocytes from adults...
  88. ncbi request reprint Suppression of BRAF(V599E) in human melanoma abrogates transformation
    Sunil R Hingorani
    Abramson Family Cancer Research Institute at the University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Cancer Res 63:5198-202. 2003
    ..Thus, when present, BRAF(V599E) appears to be essential for melanoma cell viability and transformation and, therefore, represents an attractive therapeutic target in the majority of melanomas that harbor the mutation...
  89. pmc Epidermal growth factor receptor and mutant p53 expand an esophageal cellular subpopulation capable of epithelial-to-mesenchymal transition through ZEB transcription factors
    Shinya Ohashi
    Gastroenterology Division, Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Cancer Res 70:4174-84. 2010
    ....
  90. ncbi request reprint CXC chemokine ligand 12 (stromal cell-derived factor 1 alpha) and CXCR4-dependent migration of CTLs toward melanoma cells in organotypic culture
    Tianqian Zhang
    The Wistar Institute, Philadelphia, PA 19104, USA
    J Immunol 174:5856-63. 2005
    ....
  91. ncbi request reprint Melanoma development and progression: a conspiracy between tumor and host
    Mei Yu Hsu
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104 4283, USA
    Differentiation 70:522-36. 2002
    ..Defining intercellular molecular dialogues in human skin promises to provide key information for the development of novel treatment strategies that target the functional unit of stroma and tumor...
  92. ncbi request reprint The many faces of Notch signaling in skin-derived cells
    Chelsea C Pinnix
    The Wistar Institute, Philadelphia, PA, USA
    Pigment Cell Res 20:458-65. 2007
    ..We will review the Notch signaling literature as it relates to skin homeostasis, melanocytic stem cells and melanoma tumorigenesis...
  93. pmc Raf inhibitor stabilizes receptor for the type I interferon but inhibits its anti-proliferative effects in human malignant melanoma cells
    K G Suresh Kumar
    Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Cancer Biol Ther 6:1437-41. 2007
    ..We discuss the implications of this result for combination therapy with BAY 43-9006 and IFNalpha in melanoma patients...
  94. ncbi request reprint Cell-surface proteolysis, growth factor activation and intercellular communication in the progression of melanoma
    Thomas Bogenrieder
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Crit Rev Oncol Hematol 44:1-15. 2002
    ....
  95. ncbi request reprint Differentiation of normal skin and melanoma using high resolution hyperspectral imaging
    David T Dicker
    Laboratory of Molecular Oncology and Cell Cycle Regulation, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA
    Cancer Biol Ther 5:1033-8. 2006
    ..This would assist multiple laboratories to participate in the input and retrieval of target spectral information...
  96. pmc Regulation of Notch1 and Dll4 by vascular endothelial growth factor in arterial endothelial cells: implications for modulating arteriogenesis and angiogenesis
    Zhao Jun Liu
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Mol Cell Biol 23:14-25. 2003
    ..This study provides the first evidence for regulation of Notch/Delta gene expression by an angiogenic growth factor and insight into the critical role of Notch signaling in arteriogenesis and angiogenesis...
  97. ncbi request reprint p53 alone or in combination with antisense cyclin D1 induces apoptosis and reduces tumor size in human melanoma
    Edward R Sauter
    Tumor Biology Program, The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Cancer Gene Ther 9:807-12. 2002
    ..In summary, combining the separately effective treatment vectors p53 and AS CD1 led to an enhanced growth-suppressive and apoptotic effect, supporting a role for combination gene therapy to treat human malignant melanoma...
  98. ncbi request reprint Defining the conditions for the generation of melanocytes from human embryonic stem cells
    Dong Fang
    Program of Molecular and Cellular Oncogenesis, The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Stem Cells 24:1668-77. 2006
    ..In summary, we have shown for the first time the differentiation of hESCs into melanocytes. This method provides a novel in vitro system for studying the development biology of human melanocytes...
  99. pmc Mechanism for elimination of a tumor suppressor: aberrant splicing of a brain-specific exon causes loss of function of Bin1 in melanoma
    K Ge
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 96:9689-94. 1999
    ..Our findings suggest that aberrant splicing of Bin1 may contribute to melanoma progression, and they define a mechanism by which the activity of a tumor suppressor can be eliminated in cells...
  100. ncbi request reprint The prevalence of interferon-alpha transcription defects in malignant melanoma
    Karen L Price
    RAFT Institute of Plastic Surgery, Mount Vernon Hospital, Northwood, Middlesex HA6 2RN, UK
    Melanoma Res 15:91-8. 2005
    ..These defects were found to occur in cells isolated from early melanomas, lending support to the hypothesis that IFN-alpha has a role in the aetiology of malignant melanoma...
  101. ncbi request reprint Truncation of activated leukocyte cell adhesion molecule: a gateway to melanoma metastasis
    Leon C L T van Kempen
    Department of Biochemistry NCMLS, University of Nijmegen, Nijmegen, The Netherlands
    J Invest Dermatol 122:1293-301. 2004
    ..ALCAM induction could, thus, provide an attractive target for proteolysis as a part of a more complex cellular program that couples growth and migration and facilitates dissemination...

Research Grants37

  1. Second International Melanoma Research Congress
    Meenhard Herlyn; Fiscal Year: 2004
    ..This second congress will strengthen the field and solidify the ties between the different research and clinical communities. ..
  2. Biology of Melanoma Matastasis
    Meenhard Herlyn; Fiscal Year: 2007
    ..These studies should lead to a better understanding of the tumor-specific signatures in pathways associated with invasion and metastasis. ..
  3. Cell-Cell Communication During Melanoma Development
    Meenhard Herlyn; Fiscal Year: 2007
    ..These studies will demonstrate how dysregulation of homeostasis results in tumor formation and targeting subpopulations of malignant cells leads to successful therapy. ..
  4. EXPERIMENTAL TRANSFORMATION OF HUMAN MELANOCYTES IN VIVO
    Meenhard Herlyn; Fiscal Year: 2007
    ..Our unique model allows a systematic dissection of the biological and molecular events leading to human melanoma formation. ..
  5. SPORE in Skin Cancer
    Meenhard Herlyn; Fiscal Year: 2007
    ..CTCL is addressed in one Project and one Pilot Study, and SCC in one Pilot Study. This endeavor represents the synergistic integration of well-established individual research programs towards our collective goals. ..
  6. Cell-Cell Communication During Melanoma Development
    Meenhard Herlyn; Fiscal Year: 2009
    ..These studies will demonstrate how dysregulation of homeostasis results in tumor formation and targeting subpopulations of malignant cells leads to successful therapy. ..
  7. CELL/CELL COMMUNICATION DURING MELANOMA PROGRESSION
    Meenhard Herlyn; Fiscal Year: 2000
    ..This proposal addresses the biological significance of inter-cellular signaling during distinct steps of melanoma tumor progression. ..
  8. METASTASIS--BIOLOGICAL PHENOTYPE AND MODELS FOR THERAPY
    Meenhard Herlyn; Fiscal Year: 2002
    ....
  9. EXPERIMENTAL TRANSFORMATION OF HUMAN MELANOCYTES IN VIVO
    Meenhard Herlyn; Fiscal Year: 2003
    ..Our unique in vitro/in vivo models are suitable to investigate etiology, early detection, and prevention of human melanoma. ..
  10. CELL CELL COMMUNICATION DURING MELANOMA DEVELOPMENT
    Meenhard Herlyn; Fiscal Year: 2004
    ..As additional signaling pathways are being defined, therapeutic strategies that would mimic the regulatory control of keratinocytes over E-cadherin-expressing melanoma cell will be developed as a long-term objective. ..
  11. Cell-Cell Communication During Melanoma Development
    Meenhard Herlyn; Fiscal Year: 2010
    ..These studies will demonstrate how dysregulation of homeostasis results in tumor formation and targeting subpopulations of malignant cells leads to successful therapy. ..