Research Topics
Genomes and GenesSpecies | MARY HEEBSummaryAffiliation: The Scripps Research Institute Country: USA Publications
Research Grants
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Detail Information
Publications
Platelet protein S directly inhibits procoagulant activity on platelets and microparticlesF Stavenuiter
Mary J Heeb, MEM276, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 N Torrey Pines Rd, La Jolla, CA 92037, USA, Tel 1 858 784 2185, Fax 1 858 784 2113, E mail
Thromb Haemost 109:229-37. 2013..Neutralising antibodies against APC and TFPI had no effect on the PS-direct of pltPS or pdPS on platelets. This study indicates that pltPS may be an essential pool of PS that counterbalances procoagulant activities on platelets...- Antithrombotic activity of protein S infused without activated protein C in a baboon thrombosis modelM J Heeb
Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 N Torrey Pines Rd, La Jolla, CA 92037, USA
Thromb Haemost 107:690-8. 2012..Thus, infused human Zn2+-containing ProS is antithrombotic in primates, and may have therapeutic potential even in protein C-deficient human patients... - Plasma protein S contains zinc essential for efficient activated protein C-independent anticoagulant activity and binding to factor Xa, but not for efficient binding to tissue factor pathway inhibitorMary J Heeb
Department of Molecular and Experimental Medicine, MEM276, Scripps Research Institute, 10550 N Torrey Pines Rd, La Jolla, CA 92037, USA
FASEB J 23:2244-53. 2009..Thus, Zn(2+) is required for PS-direct and efficient FXa binding and may play a role in stabilizing PS conformation... 
Plasma contains protein S monomers and multimers with similar direct anticoagulant activityM J Heeb
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
J Thromb Haemost 4:2215-22. 2006..Protein S (PS) has activated protein C-independent, direct anticoagulant activity (PS-direct). We reported that both multimers and monomers of affinity-purified PS have PS-direct similar to that in plasma, in contrast to another report...- Protein S multimers and monomers each have direct anticoagulant activityM J Heeb
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
J Thromb Haemost 4:385-91. 2006..We independently examined the relative PS-direct and affinity for phospholipids of monomeric and multimeric PS and we obtained contrasting results... - Down-regulation of factor IXa in the factor Xase complex by protein Z-dependent protease inhibitorMary J Heeb
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
J Biol Chem 280:33819-25. 2005..ZPI may be an unusual physiologic regulator of both the intrinsic FXase and the prothrombinase complexes... - Direct anticoagulant activity of protein S-C4b binding protein complex in Heerlen heterozygotes and normalsM J Heeb
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
J Thromb Haemost 2:1766-73. 2004..Protein S-Heerlen lacks a carbohydrate group, leading to low plasma free protein S levels, but normal levels of PS-C4BP... - Activated protein C-dependent and -independent anticoagulant activities of protein S have different structural requirementsMary J Heeb
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
Blood Cells Mol Dis 29:190-9. 2002..Thus, the APC-cofactor activity protein S is significantly more sensitive to structural changes in the TSR than is the APC-independent activity of protein S... - Low protein Z levels and risk of ischemic stroke: differences by diabetic status and genderMary J Heeb
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
Blood Cells Mol Dis 29:139-44. 2002..015). Protein Z is suggested to be a physiologic downregulator of blood coagulation and low protein Z levels are associated with increased risk of ischemic stroke, particularly in males and in the absence of diabetes... - C-terminal residues 621-635 of protein S are essential for binding to factor VaM J Heeb
Departments of Molecular and Experimental Medicine and Vascular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
J Biol Chem 274:36187-92. 1999..These results show that the C-terminal region of protein S containing residues 621-635 is essential for binding of protein S to Factor Va and that this interaction contributes to anticoagulant action... - Binding sites for blood coagulation factor Xa and protein S involving residues 493-506 in factor VaM J Heeb
Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, California 92037, USA
Protein Sci 5:1883-9. 1996..Possible loss of this FVa binding site for FXa due to cleavage at Arg 506 by APC may help explain why this cleavage causes 40% decrease in FVa activity and facilitates inactivation of FVa... - alpha2-macroglobulin and C1-inactivator are plasma inhibitors of human glandular kallikreinM J Heeb
Departments of Molecular and Experimental Medicine and of Vascular Biology, The Scripps Research Institute, La Jolla, CA
Blood Cells Mol Dis 24:412-9. 1998..Measurement of one or more of the hK2 forms identified here may have diagnostic or prognostic potential for prostate cancer... - Importance of individual activated protein C cleavage site regions in coagulation factor V for factor Va inactivation and for factor Xa activationM J Heeb
Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, CA 92037, USA
Eur J Biochem 260:64-75. 1999.... - Binding of protein S to factor Va associated with inhibition of prothrombinase that is independent of activated protein CM J Heeb
Committee on Vascular Biology, Scripps Research Institute, La Jolla, California 92037
J Biol Chem 268:2872-7. 1993..These data show that protein S has an anticoagulant function which is independent of activated protein C and, at least in part, that this is because of its competition with prothrombin for direct binding to Factor Va... - Protein S binds to and inhibits factor XaM J Heeb
Scripps Research Institute, La Jolla, CA 92037
Proc Natl Acad Sci U S A 91:2728-32. 1994..These data demonstrate mechanisms of anticoagulant action for protein S that are independent of activated protein C and that involve direct binding to factors Xa and Va and direct inhibition of factor Xa... - Factor Va residues 311-325 represent an activated protein C binding regionSubramanian Yegneswaran
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
J Biol Chem 282:28353-61. 2007..These data suggest that peptide VP311-325 represents both an APC and fXa binding region in fVa... - Gas6, a ligand for the receptor protein-tyrosine kinase Tyro-3, is widely expressed in the central nervous systemA L Prieto
Department of Neuropharmacology CVN12, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Brain Res 816:646-61. 1999..The spatial and temporal profiles of Gas6 expression suggest that it could potentially serve as the physiologically relevant ligand for Tyro-3 in the postnatal rat nervous system... - Association of low protein Z levels with ischemic stroke in young womenMary J Heeb
Thromb Haemost 97:495-6. 2007 - Familial protein S deficiency with a variant protein S molecule in plasma and plateletsH P Schwarz
Department of Immunology, Research Institute of Scripps Clinic, La Jolla, CA 92037
Blood 74:213-21. 1989.... - Both protein S and Gas6 stimulate outer segment phagocytosis by cultured rat retinal pigment epithelial cellsMichael O Hall
Jules Stein Eye Institute, UCLA Medical Center, 100 Stein Plaza, Los Angeles, CA 90095 7008, USA
Exp Eye Res 81:581-91. 2005..The existence of redundant ligands for Mer-dependent OS phagocytosis underscores the critical role of this process in the maintenance of retinal function...
Research Grants
- New Aspects of Protein S Anticoagulant ActivityMARY HEEB; Fiscal Year: 2009..Insights may lead to new treatments, or measures that prevent thrombosis. ..
- Novel Anticoagulant MechanismsMARY HEEB; Fiscal Year: 2006..We will find whether a ZPI polymorphism we identified affects function. ..
- FORMS OF PROSTATE SPECIFIC ANTIGEN AND HK2 IN CANCERMARY HEEB; Fiscal Year: 2003..Techniques will include protein purification, antibody and ELISA development, immunoblotting, enzyme activity assays, and peptide synthesis. ..
- New Aspects of Protein S Anticoagulant ActivityMary J Heeb; Fiscal Year: 2010..Insights may lead to new treatments, or measures that prevent thrombosis. ..