M H Ginsberg

Summary

Affiliation: The Scripps Research Institute
Country: USA

Publications

  1. ncbi request reprint A membrane-distal segment of the integrin alpha IIb cytoplasmic domain regulates integrin activation
    M H Ginsberg
    Department of Vascular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 276:22514-21. 2001
  2. pmc Physical mapping of genes in somatic cell radiation hybrids by comparative genomic hybridization to cDNA microarrays
    Johann Y Lin
    Department of Vascular Biology, The Scripps Research Institute, 10550 N, Torrey Pines Road, La Jolla, CA 92037, USA
    Genome Biol 3:RESEARCH0026. 2002
  3. ncbi request reprint Integrin cytoplasmic domain-binding proteins
    S Liu
    Department of Vascular Biology, VB 2, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Sci 113:3563-71. 2000
  4. ncbi request reprint Increased filamin binding to beta-integrin cytoplasmic domains inhibits cell migration
    D A Calderwood
    Department of Vascular Biology, The Scripps Research Institute, 10550 N Torrey Pines Rd, La Jolla, California 92037, USA
    Nat Cell Biol 3:1060-8. 2001
  5. ncbi request reprint Phosphorylation of the integrin alpha 4 cytoplasmic domain regulates paxillin binding
    J Han
    Department of Vascular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 276:40903-9. 2001
  6. ncbi request reprint Activation of Syk protein tyrosine kinase through interaction with integrin beta cytoplasmic domains
    D G Woodside
    Department of Vascular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Curr Biol 11:1799-804. 2001
  7. pmc Affinity modulation of the alpha IIb beta 3 integrin (platelet GPIIb-IIIa) is an intrinsic property of the receptor
    T E O'Toole
    Research Institute of Scripps Clinic, La Jolla, California 92037
    Cell Regul 1:883-93. 1990
  8. ncbi request reprint Calpain cleavage promotes talin binding to the beta 3 integrin cytoplasmic domain
    B Yan
    Department of Vascular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 276:28164-70. 2001
  9. ncbi request reprint Calpain cleavage of integrin beta cytoplasmic domains
    M Pfaff
    Department of Vascular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    FEBS Lett 460:17-22. 1999
  10. ncbi request reprint Binding of Paxillin to the alpha 9 Integrin Cytoplasmic Domain Inhibits Cell Spreading
    S Liu
    Department of Vascular Biology, The Scripps Research Institute, La Jolla, California 92037
    J Biol Chem 276:37086-92. 2001

Collaborators

Detail Information

Publications88

  1. ncbi request reprint A membrane-distal segment of the integrin alpha IIb cytoplasmic domain regulates integrin activation
    M H Ginsberg
    Department of Vascular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 276:22514-21. 2001
    ..Thus, this region of the alpha(IIb) tail causes the beta(3) tail to resemble that of beta(3)(Y747A) and suppresses activation of the integrin...
  2. pmc Physical mapping of genes in somatic cell radiation hybrids by comparative genomic hybridization to cDNA microarrays
    Johann Y Lin
    Department of Vascular Biology, The Scripps Research Institute, 10550 N, Torrey Pines Road, La Jolla, CA 92037, USA
    Genome Biol 3:RESEARCH0026. 2002
    ..Here we report experiments that establish the validity and efficacy of the methodology...
  3. ncbi request reprint Integrin cytoplasmic domain-binding proteins
    S Liu
    Department of Vascular Biology, VB 2, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Sci 113:3563-71. 2000
    ..These and other proteins (e.g. FAK, ILK and novel proteins such as TAP20) might also link integrins to signaling mechanisms and, in some cases (e.g. JAB1) mediate integrin-dependent gene regulation...
  4. ncbi request reprint Increased filamin binding to beta-integrin cytoplasmic domains inhibits cell migration
    D A Calderwood
    Department of Vascular Biology, The Scripps Research Institute, 10550 N Torrey Pines Rd, La Jolla, California 92037, USA
    Nat Cell Biol 3:1060-8. 2001
    ..Thus, tight filamin binding restricts integrin-dependent cell migration by inhibiting transient membrane protrusion and cell polarization...
  5. ncbi request reprint Phosphorylation of the integrin alpha 4 cytoplasmic domain regulates paxillin binding
    J Han
    Department of Vascular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 276:40903-9. 2001
    ..Consequently, alpha4 phosphorylation is a biochemical mechanism to modulate paxillin binding to alpha4 integrins with consequent regulation of alpha4 integrin-dependent cellular functions...
  6. ncbi request reprint Activation of Syk protein tyrosine kinase through interaction with integrin beta cytoplasmic domains
    D G Woodside
    Department of Vascular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Curr Biol 11:1799-804. 2001
    ..Thus, the direct binding of Syk kinase to the integrin beta3 cytoplasmic tail is a novel and functionally significant mechanism for the regulation of this important non-receptor tyrosine kinase...
  7. pmc Affinity modulation of the alpha IIb beta 3 integrin (platelet GPIIb-IIIa) is an intrinsic property of the receptor
    T E O'Toole
    Research Institute of Scripps Clinic, La Jolla, California 92037
    Cell Regul 1:883-93. 1990
    ..Furthermore, they indicate that the activation state of alpha IIb beta 3 is a property of the receptor itself rather than of the surrounding cell membrane microenvironment...
  8. ncbi request reprint Calpain cleavage promotes talin binding to the beta 3 integrin cytoplasmic domain
    B Yan
    Department of Vascular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 276:28164-70. 2001
    ..Furthermore, they suggest that talin cleavage by calpain may contribute to the effects of the protease on the clustering and activation of integrins...
  9. ncbi request reprint Calpain cleavage of integrin beta cytoplasmic domains
    M Pfaff
    Department of Vascular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    FEBS Lett 460:17-22. 1999
    ..Thus susceptibility to calpain cleavage is common to integrin beta subunits, can be induced in intact cells, and appears to favor regions surrounding two conserved NXXY motifs...
  10. ncbi request reprint Binding of Paxillin to the alpha 9 Integrin Cytoplasmic Domain Inhibits Cell Spreading
    S Liu
    Department of Vascular Biology, The Scripps Research Institute, La Jolla, California 92037
    J Biol Chem 276:37086-92. 2001
    ....
  11. ncbi request reprint Integrin beta cytoplasmic domains differentially bind to cytoskeletal proteins
    M Pfaff
    Department of Vascular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 273:6104-9. 1998
    ..The structural specificity of these associations suggests that these mimics offer a useful approach for the analysis of the interactions and structure of the integrin cytoplasmic face...
  12. ncbi request reprint The death effector domain of PEA-15 is involved in its regulation of integrin activation
    J W Ramos
    Department of Vascular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 273:33897-900. 1998
    ..In contrast, the DED of PEA-15 is essential for its capacity to reverse suppression of integrin activation. Thus, certain DED-containing proteins can regulate integrin activation as opposed to apoptotic protease cascades...
  13. pmc Affinity modulation of platelet integrin alphaIIbbeta3 by beta3-endonexin, a selective binding partner of the beta3 integrin cytoplasmic tail
    H Kashiwagi
    Department of Vascular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Cell Biol 137:1433-43. 1997
    ..By analogy, the adhesive function of platelets may be regulated by such protein-protein interactions at the level of the cytoplasmic tails of alphaIIbbeta3...
  14. ncbi request reprint Beta 3 integrin-mediated fibrin clot retraction by nucleated cells: differing behavior of alpha IIb beta 3 and alpha v beta 3
    Y P Chen
    Department of Vascular Biology, Scripps Research Institute, La Jolla, CA 92037, USA
    Blood 86:2606-15. 1995
    ....
  15. ncbi request reprint Integrin activation by talin
    B I Ratnikov
    Department of Medicine, University of California San Diego, La Jolla, CA 92093 0726, USA
    J Thromb Haemost 3:1783-90. 2005
    ..These findings indicate that talin is likely to be at the center of converging signaling pathways regulating integrin activation...
  16. pmc Death effector domain protein PEA-15 potentiates Ras activation of extracellular signal receptor-activated kinase by an adhesion-independent mechanism
    J W Ramos
    Department of Vascular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Mol Biol Cell 11:2863-72. 2000
    ..In contrast, the DED of PEA-15 is essential for its capacity to activate ERK. The ability of PEA-15 to simultaneously inhibit apoptosis and potentiate Ras-to-Erk signaling may be of importance for oncogenic processes...
  17. ncbi request reprint A beta 3 integrin mutation abolishes ligand binding and alters divalent cation-dependent conformation
    J C Loftus
    Committee on Vascular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037
    Science 249:915-8. 1990
    ..The spacing of these residues aligns with the calcium-binding residues in EF hand proteins, suggesting interaction with receptor-bound divalent cation as a mechanism of ligand binding common to all integrins...
  18. ncbi request reprint Binding of paxillin to alpha4 integrins modifies integrin-dependent biological responses
    S Liu
    Department of Vascular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Nature 402:676-81. 1999
    ..Thus, the tight association of paxillin with the alpha4 tail leads to distinct biochemical and biological responses to integrin-mediated cell adhesion...
  19. ncbi request reprint Complementation of dominant suppression implicates CD98 in integrin activation
    C A Fenczik
    Department of Vascular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Nature 390:81-5. 1997
    ..These data indicate that CD98 is involved in regulating integrin affinity, and validate an unbiased genetic approach to analysing integrin signalling pathways...
  20. pmc Regulation of the pp72syk protein tyrosine kinase by platelet integrin alpha IIb beta 3
    J Gao
    Department of Vascular Biology, Scripps Research Institute, La Jolla, CA 92037, USA
    EMBO J 16:6414-25. 1997
    ..Furthermore, there are fundamental differences in the activation of Syk by alpha IIb beta 3 and immune response receptors, suggesting a unique role for integrins in Syk function...
  21. ncbi request reprint Influence of fibrillar collagen structure on the mechanisms of platelet thrombus formation under flow
    B Savage
    Roon Research Center for Arteriosclerosis and Thrombosis, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    Blood 94:2704-15. 1999
    ..In addition, collagen structure may regulate the extent and affinity of the binding under flow of plasma components such as von Willebrand factor and/or other alpha(IIb)beta(3) ligands...
  22. ncbi request reprint A unique recognition site mediates the interaction of fibrinogen with the leukocyte integrin Mac-1 (CD11b/CD18)
    D C Altieri
    Department of Immunology, Research Institute of Scripps Clinic, La Jolla, California 92037
    J Biol Chem 265:12119-22. 1990
    ..We conclude that fibrinogen interacts with the leukocyte integrin Mac-1 through a novel recognition site that is not shared with other known integrins that function as fibrinogen receptors...
  23. ncbi request reprint Suppression of integrin activation: a novel function of a Ras/Raf-initiated MAP kinase pathway
    P E Hughes
    Department of Vascular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Cell 88:521-30. 1997
    ..Thus, regulation of integrin affinity state is a novel, transcription-independent function of a Ras-linked MAP kinase pathway that may mediate a negative feedback loop in integrin function...
  24. ncbi request reprint The affinity of integrin alpha(4)beta(1) governs lymphocyte migration
    D M Rose
    Department of Vascular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Immunol 167:2824-30. 2001
    ..Changes in this set point may specify preferred anatomical sites of integrin-dependent leukocyte emigration from the bloodstream...
  25. ncbi request reprint Integrin activation
    D G Woodside
    Department of Vascular Biology, Scripps Research Institute, La Jolla, CA 92037, USA
    Thromb Haemost 86:316-23. 2001
    ....
  26. pmc Integrin activation controls metastasis in human breast cancer
    B Felding-Habermann
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 98:1853-8. 2001
    ..Consequently, alterations within tumors that lead to the aberrant control of integrin activation are expected to adversely affect the course of human breast cancer...
  27. pmc Molecular cloning and chemical synthesis of a region of platelet glycoprotein IIb involved in adhesive function
    J C Loftus
    Department of Immunology, Research Institute of Scripps Clinic, La Jolla, CA 92037
    Proc Natl Acad Sci U S A 84:7114-8. 1987
    ..The region containing the PMI-1 epitope shows no similarity to corresponding regions of two other adhesion receptors, indicating that this portion of GPIIb may function in activities unique to the platelet receptor...
  28. ncbi request reprint Distinct domains of CD98hc regulate integrins and amino acid transport
    C A Fenczik
    Department of Vascular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 276:8746-52. 2001
    ..Furthermore, a mutant of CD98hc that lacks its integrin binding site can still promote increased isoleucine transport. Thus, these two functions of CD98hc are separable and require distinct domains of the protein...
  29. pmc Molecular cloning and preliminary characterization of a novel cytoplasmic antigen recognized by myasthenia gravis sera
    T Gordon
    Department of Basic and Clinical Research, Research Institute of Scripps Clinic, La Jolla, California 92037
    J Clin Invest 90:992-9. 1992
    ..These data indicate that gravin is an inducible, cell type-specific cytoplasmic protein and that auto-antibodies to gravin may be highly specific for MG...
  30. ncbi request reprint Association of a phospholipase A2 (14-3-3 protein) with the platelet glycoprotein Ib-IX complex
    X Du
    Department of Vascular Biology, Scripps Research Institute, La Jolla, California 92037
    J Biol Chem 269:18287-90. 1994
    ..As activation of PLA2 is an early event in GPIb-IX-mediated signaling, this result suggests that ligand occupancy of GPIb-IX may directly activate PLA2, leading to platelet activation...
  31. ncbi request reprint Calpain cleavage of the cytoplasmic domain of the integrin beta 3 subunit
    X Du
    Department of Vascular Biology, Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 270:26146-51. 1995
    ..Thus, calpain cleavage of the beta 3 cytoplasmic domain may provide a means to regulate integrin signaling functions...
  32. ncbi request reprint Networks and crosstalk: integrin signalling spreads
    Martin A Schwartz
    Division of Vascular Biology, Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Cell Biol 4:E65-8. 2002
    ..We discuss how adhesive signalling is coordinately regulated through intersecting networks. We also examine some examples of how some forms of integrin crosstalk may lead to unforeseen and potentially deleterious responses...
  33. pmc Molecular cloning and expression of the cDNA for alpha 3 subunit of human alpha 3 beta 1 (VLA-3), an integrin receptor for fibronectin, laminin, and collagen
    Y Takada
    Research Institute of Scripps Clinic, La Jolla, California 92037
    J Cell Biol 115:257-66. 1991
    ..The human alpha 3 sequence was 89% identical to hamster galactoprotein b3, and 70% similar to the chicken CSAT antigen band 2 protein partial sequence, suggesting that these two polypeptides are homologues of human alpha 3...
  34. pmc Phosphoprotein enriched in astrocytes 15 kDa (PEA-15) reprograms growth factor signaling by inhibiting threonine phosphorylation of fibroblast receptor substrate 2alpha
    Jacob R Haling
    Department of Medicine, University of California San Diego, La Jolla, CA 92093 0726, USA
    Mol Biol Cell 21:664-73. 2010
    ..Thus, PEA-15 prevents ERK1/2 localization to the plasma membrane, thereby inhibiting ERK1/2-dependent threonine phosphorylation of FRS2alpha to promote activation of the ERK1/2 MAP kinase pathway...
  35. pmc Integrin activation takes shape
    R C Liddington
    Program in Cell Adhesion, The Burnham Institute, La Jolla, CA 92037, USA
    J Cell Biol 158:833-9. 2002
    ....
  36. ncbi request reprint The phosphotyrosine binding-like domain of talin activates integrins
    David A Calderwood
    Division of Vascular Biology, Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 277:21749-58. 2002
    ..These resemblances suggest that the activation of integrins requires the presence of a beta turn at NPX(Y/F) motifs conserved in integrin beta cytoplasmic domains...
  37. ncbi request reprint PEA-15 binding to ERK1/2 MAPKs is required for its modulation of integrin activation
    Fan Li Chou
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 278:52587-97. 2003
    ..Thus, ERK1/2 are the Raf effectors for suppression of integrin activation, and PEA-15 reverses suppression by binding ERK1/2...
  38. pmc Integrin beta cytoplasmic domain interactions with phosphotyrosine-binding domains: a structural prototype for diversity in integrin signaling
    David A Calderwood
    Division of Vascular Biology, Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 100:2272-7. 2003
    ..These data establish that short integrin beta tails interact with a large number of PTB domain-containing proteins through a structurally conserved mechanism...
  39. ncbi request reprint A spontaneous mutation of integrin alpha IIb beta 3 (platelet glycoprotein IIb-IIIa) helps define a ligand binding site
    M L Bajt
    Committee on Vascular Biology, Scripps Research Institute, La Jolla, California 92037
    J Biol Chem 267:3789-94. 1992
    ..These findings suggest that substitution of beta 3 R214 by Q is responsible for the functional defect in alpha IIb beta 3 and that R214 is proximal to or part of a ligand binding domain in alpha IIb beta 3...
  40. ncbi request reprint Structural basis for phosphatidylinositol phosphate kinase type Igamma binding to talin at focal adhesions
    Jose M de Pereda
    Program on Cell Adhesion, The Burnham Institute, La Jolla, California 92037, USA
    J Biol Chem 280:8381-6. 2005
    ..Our data are consistent with the proposal that Src activity promotes a switch from integrin binding to PIPKIgamma binding that regulates focal adhesion turnover...
  41. pmc CD98hc (SLC3A2) mediates integrin signaling
    Chloe C Feral
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 102:355-60. 2005
    ..Thus, CD98hc is an integrin-associated protein that mediates integrin-dependent signals, which promote tumorigenesis...
  42. ncbi request reprint An alpha4 integrin-paxillin-Arf-GAP complex restricts Rac activation to the leading edge of migrating cells
    Naoyuki Nishiya
    Department of Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0726, USA
    Nat Cell Biol 7:343-52. 2005
    ..These findings establish a mechanism for the spatial localization of Rac activity to enhance cell migration...
  43. pmc Phosphorylation of phosphoprotein enriched in astrocytes (PEA-15) regulates extracellular signal-regulated kinase-dependent transcription and cell proliferation
    Joseph Krueger
    University of California San Diego, La Jolla, CA 92093 0726, USA
    Mol Biol Cell 16:3552-61. 2005
    ..These results define a novel posttranslational mechanism for controlling the subcellular localization of ERK1/2 and for specifying the output of MAP kinase signaling...
  44. pmc Integrin alpha4beta1 promotes focal adhesion kinase-independent cell motility via alpha4 cytoplasmic domain-specific activation of c-Src
    Datsun A Hsia
    The Scripps Research Institute, Department of Immunology, IMM21, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Mol Cell Biol 25:9700-12. 2005
    ....
  45. ncbi request reprint Alpha4 integrins are type I cAMP-dependent protein kinase-anchoring proteins
    Chinten James Lim
    Department of Medicine, University of California San Diego, La Jolla, CA 92093, USA
    Nat Cell Biol 9:415-21. 2007
    ....
  46. pmc Differences in regulation of Drosophila and vertebrate integrin affinity by talin
    Teresa L Helsten
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    Mol Biol Cell 19:3589-98. 2008
    ....
  47. pmc RIAM activates integrins by linking talin to ras GTPase membrane-targeting sequences
    Ho Sup Lee
    Department of Medicine, University of California San Diego, La Jolla, California 92093 0726, USA
    J Biol Chem 284:5119-27. 2009
    ..These data establish the mechanism whereby MRL proteins interact with both talin and Ras GTPases to activate integrins...
  48. ncbi request reprint Competition for talin results in trans-dominant inhibition of integrin activation
    David A Calderwood
    Deptartment of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 279:28889-95. 2004
    ..Thus, the sequestration of talin by the suppressive species is both necessary and sufficient for trans-dominant inhibition of integrin activation...
  49. ncbi request reprint C-terminal sequences in R-Ras are involved in integrin regulation and in plasma membrane microdomain distribution
    Malene Hansen
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Biochem Biophys Res Commun 311:829-38. 2003
    ..However, functionally distinct H-Ras/R-Ras chimeras containing different C-terminal R-Ras segments localized to lipid rafts irrespective of their integrin phenotype...
  50. pmc Spatial restriction of alpha4 integrin phosphorylation regulates lamellipodial stability and alpha4beta1-dependent cell migration
    Lawrence E Goldfinger
    Department of Cell Biology, Division of Vascular Biology, The Scripps Research Institute, 10550 N Torrey Pines Road, VB 2 La Jolla, CA 92037, USA
    J Cell Biol 162:731-41. 2003
    ..These results show that topographically specific integrin phosphorylation can control cell migration and polarization by spatial segregation of adaptor protein binding...
  51. ncbi request reprint The N-terminal SH2 domains of Syk and ZAP-70 mediate phosphotyrosine-independent binding to integrin beta cytoplasmic domains
    Darren G Woodside
    Division of Vascular Biology, Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 277:39401-8. 2002
    ..Thus, the binding of Syk and ZAP-70 to integrin beta cytoplasmic domains represents a novel phosphotyrosine-independent interaction mediated by their N-terminal SH2 domains...
  52. pmc Src kinase activation by direct interaction with the integrin beta cytoplasmic domain
    Elena G Arias-Salgado
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 100:13298-302. 2003
    ..The data provide a paradigm for integrin regulation of Src and a molecular basis for the similar functional defects of osteoclasts or platelets from mice lacking beta3 integrins or c-Src...
  53. ncbi request reprint Ras GTPases: integrins' friends or foes?
    Kayoko Kinbara
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Nat Rev Mol Cell Biol 4:767-76. 2003
    ..The Ras family of small G proteins, which includes H-ras, R-ras and Rap, are important elements in cellular signalling pathways that control integrin function...
  54. ncbi request reprint Integrin alpha 4 beta 1-dependent T cell migration requires both phosphorylation and dephosphorylation of the alpha 4 cytoplasmic domain to regulate the reversible binding of paxillin
    Jaewon Han
    Department of Cell Biology, Division of Vascular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 278:34845-53. 2003
    ..These data establish that optimal alpha 4-mediated cell migration requires both phosphorylation and dephosphorylation of the alpha 4 cytoplasmic domain to regulate the reversible binding of paxillin...
  55. pmc Mechanisms and consequences of agonist-induced talin recruitment to platelet integrin alphaIIbbeta3
    Naohide Watanabe
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 181:1211-22. 2008
    ..Thus, talin recruitment to alphaIIbbeta3 by RIAM mediates agonist-induced alphaIIbbeta3 activation, with implications for hemostasis and thrombosis...
  56. pmc Suppression of integrin activation by activated Ras or Raf does not correlate with bulk activation of ERK MAP kinase
    Paul E Hughes
    The Division of Vascular Biology, Department of Cell Biology The Scripps Research Institute, La Jolla, California 92037, USA
    Mol Biol Cell 13:2256-65. 2002
    ..Thus, the suppression of integrin activation is a Raf kinase induced regulatory event that can be mediated independently of bulk activation of the ERK MAP-kinase pathway...
  57. pmc A small molecule inhibitor of alpha4 integrin-dependent cell migration
    Jongkook Lee
    Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Bioorg Med Chem 17:977-80. 2009
    ..This molecule should serve as a useful tool to study alpha4 integrin signaling and may lead to new therapeutics for the treatment of autoimmune diseases...
  58. doi request reprint The final steps of integrin activation: the end game
    Sanford J Shattil
    Department of Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA
    Nat Rev Mol Cell Biol 11:288-300. 2010
    ..Recent progress provides insight into the structure of integrin transmembrane domains, and reveals how the final steps of integrin activation are mediated by integrin-binding proteins such as talins and kindlins...
  59. pmc Talin is required for integrin-mediated platelet function in hemostasis and thrombosis
    Brian G Petrich
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    J Exp Med 204:3103-11. 2007
    ..These data establish that platelet talin plays a crucial role in hemostasis and provide the first proof that talin is required for the activation and function of mammalian alpha2beta1 and alphaIIbbeta3 integrins in vivo...
  60. pmc KRIT-1/CCM1 is a Rap1 effector that regulates endothelial cell cell junctions
    Angela Glading
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 179:247-54. 2007
    ..Thus, Rap1 increases KRIT-1 targeting to endothelial cell-cell junctions where it suppresses stress fibers and stabilizes junctional integrity...
  61. pmc Integrin-mediated protein kinase A activation at the leading edge of migrating cells
    Chinten J Lim
    Department of Medicine, University of California San Diego, La Jolla, CA 92093 0726, USA
    Mol Biol Cell 19:4930-41. 2008
    ..In sum, we have visualized compartment-specific PKA activation in migrating cells and used it to reveal that adhesion-mediated localized activation of PKA is an early step in directional cell migration...
  62. pmc Interactions of platelet integrin alphaIIb and beta3 transmembrane domains in mammalian cell membranes and their role in integrin activation
    Chungho Kim
    Department of Medicine, University of California San Diego, La Jolla, CA 92093, USA
    Blood 113:4747-53. 2009
    ..These studies establish the preferential heterodimeric interactions of integrin alpha(IIb)beta(3) TMD tails in mammalian cell membranes and document their role in integrin signaling...
  63. pmc CD98hc facilitates B cell proliferation and adaptive humoral immunity
    Joseph Cantor
    Department of Medicine, University of California San Diego, La Jolla, USA
    Nat Immunol 10:412-9. 2009
    ..Thus, CD98hc supports integrin-dependent rapid proliferation of B cells. We propose that the advantage of adaptive immunity favored the appearance of CD98hc in vertebrates...
  64. pmc Dependence of proliferative vascular smooth muscle cells on CD98hc (4F2hc, SLC3A2)
    Per Fogelstrand
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    J Exp Med 206:2397-406. 2009
    ....
  65. ncbi request reprint A fragment of paxillin binds the alpha 4 integrin cytoplasmic domain (tail) and selectively inhibits alpha 4-mediated cell migration
    Shouchun Liu
    Department of Vascular Biology and Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 277:20887-94. 2002
    ..Thus, paxillin binding to the alpha(4) tail leads to enhanced cell migration and inhibition of the alpha(4)-paxillin interaction selectively blocks the alpha4-dependent cellular responses...
  66. pmc Recreation of the terminal events in physiological integrin activation
    Feng Ye
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 188:157-73. 2010
    ....
  67. pmc A small molecule that inhibits the interaction of paxillin and alpha 4 integrin inhibits accumulation of mononuclear leukocytes at a site of inflammation
    Christiane Kummer
    Department of Medicine, University of California at San Diego, La Jolla, California 92093, USA
    J Biol Chem 285:9462-9. 2010
    ....
  68. pmc Cerebral cavernous malformations proteins inhibit Rho kinase to stabilize vascular integrity
    Rebecca A Stockton
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    J Exp Med 207:881-96. 2010
    ....
  69. pmc CD98hc (SLC3A2) participates in fibronectin matrix assembly by mediating integrin signaling
    Chloe C Feral
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 178:701-11. 2007
    ..CD98hc expression can vary widely; our data show that these variations in CD98hc expression can control the capacity of cells to assemble an Fn matrix, a process important in development, wound healing, and tumorigenesis...
  70. ncbi request reprint Transmembrane domain helix packing stabilizes integrin alphaIIbbeta3 in the low affinity state
    Anthony W Partridge
    The Department of Medicine, University of California San Diego, La Jolla, California 92093 0726, USA
    J Biol Chem 280:7294-300. 2005
    ..Therefore, we propose that helical packing of the alpha and beta TM domains forms a clasp that regulates integrin activation...
  71. ncbi request reprint Integrin regulation
    Mark H Ginsberg
    Department of Medicine, University of California San Diego, La Jolla, California 92093, USA
    Curr Opin Cell Biol 17:509-16. 2005
    ..However, a key unresolved question is how integrins propagate signals across the plasma membrane...
  72. ncbi request reprint An experimentally derived database of candidate Ras-interacting proteins
    Lawrence E Goldfinger
    Division of Rheumatology, Department of Medicine, University of California, San Diego, La Jolla, California 92093, USA
    J Proteome Res 6:1806-11. 2007
    ..R-Ras and Rap1A each associated with various signaling molecules, many of which are membrane-associated. Thus, we have established the first database of potential Ras interactors in mammalian cells...
  73. ncbi request reprint Neurobiology: New connections between integrins and axon guidance
    Tetsuya Nakamoto
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Curr Biol 14:R121-3. 2004
    ..Recent reports have described multiple paradigms by which these molecules interact with integrin adhesion receptors in and outside the neuronal tissues...
  74. ncbi request reprint PEA-15 inhibits tumor cell invasion by binding to extracellular signal-regulated kinase 1/2
    Angela Glading
    Department of Medicine, University of California San Diego, The Scripps Research Institute, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Cancer Res 67:1536-44. 2007
    ..These results reveal that PEA-15 regulates cancer cell invasion via its ability to bind ERK1/2 and indicate that nuclear entry of ERK1/2 is important in tumor behavior...
  75. pmc RLIP76 (RalBP1) is an R-Ras effector that mediates adhesion-dependent Rac activation and cell migration
    Lawrence E Goldfinger
    Division of Rheumatology, Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 174:877-88. 2006
    ..Thus, we identify a novel R-Ras effector, RLIP76, which links R-Ras to adhesion-induced Rac activation through a GTPase cascade that mediates cell spreading and migration...
  76. ncbi request reprint Reconstructing and deconstructing agonist-induced activation of integrin alphaIIbbeta3
    Jaewon Han
    Department of Medicine, University of California, San Diego, La Jolla, California 92093, USA
    Curr Biol 16:1796-806. 2006
    ..Many different signaling molecules that regulate integrin affinity have been described, but a pathway that connects agonist stimulation to talin binding and activation has not been mapped...
  77. ncbi request reprint The Kindler syndrome protein is regulated by transforming growth factor-beta and involved in integrin-mediated adhesion
    Susanne Kloeker
    Department of Oncological Sciences, Huntsman Cancer Institute, Salt Lake City, Utah 84112 5550, USA
    J Biol Chem 279:6824-33. 2004
    ..Additionally, kindlerin is required for normal cell spreading. Taken together, these data suggest a role for kindlerin in mediating cell processes that depend on integrins...
  78. ncbi request reprint Talin forges the links between integrins and actin
    David A Calderwood
    Nat Cell Biol 5:694-7. 2003
  79. ncbi request reprint Cell migration: integrating signals from front to back
    Anne J Ridley
    Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London W1W 7BS, UK
    Science 302:1704-9. 2003
    ....
  80. pmc Genetic interaction between integrins and moleskin, a gene encoding a Drosophila homolog of importin-7
    Scott E Baker
    Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721, USA
    Genetics 162:285-96. 2002
    ..Genetic tests suggest that the moleskin suppression of wing blisters is not directly related to inhibition of D-ERK nuclear import. These data are discussed with respect to the possible regulation of integrin function by cytoplasmic ERK...
  81. ncbi request reprint Alpha4 integrins and the immune response
    David M Rose
    Division of Rheumatology, Allergy, and Immunology, Department of Medicine, The University of California, San Diego, California, USA
    Immunol Rev 186:118-24. 2002
    ..Furthermore, this protein-protein interaction may provide novel targets for the modulation of the immune response...
  82. ncbi request reprint R-Ras C-terminal sequences are sufficient to confer R-Ras specificity to H-Ras
    Malene Hansen
    Department of Molecular Cell Biology, Institute of Molecular Biology, University of Copenhagen, Ă˜ster Farimagsgade 2A, Denmark
    Oncogene 21:4448-61. 2002
    ....
  83. ncbi request reprint Phospholipase C epsilon suppresses integrin activation
    Yatish Lad
    Medical Research Council Centre for Inflammation Research, Queen s Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh EH16 4SA, Scotland, United Kingdom
    J Biol Chem 281:29501-12. 2006
    ..These results show that H-Ras suppresses integrin affinity via independent Raf and PLCepsilon signaling pathways and demonstrate a new physiological function for PLCepsilon in the regulation of integrin activation...
  84. pmc Localized alpha4 integrin phosphorylation directs shear stress-induced endothelial cell alignment
    Lawrence E Goldfinger
    Sol Sherry Thrombosis Research Center and Department of Anatomy and Cell Biology, Temple University School of Medicine, 3400 N Broad Street, OMS 415, Philadelphia, PA 19140, USA
    Circ Res 103:177-85. 2008
    ..We thereby show that shear-induced PKA-dependent alpha4 integrin phosphorylation at the downstream edge of endothelial cells promotes localized Rac1 activation, which in turn directs cytoskeletal alignment in response to shear stress...
  85. ncbi request reprint Structural basis of integrin activation by talin
    Kate L Wegener
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, England, UK
    Cell 128:171-82. 2007
    ....
  86. pmc Syk, c-Src, the alphavbeta3 integrin, and ITAM immunoreceptors, in concert, regulate osteoclastic bone resorption
    Wei Zou
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110, USA
    J Cell Biol 176:877-88. 2007
    ..Thus, in conjunction with ITAM-bearing proteins, Syk, c-Src, and alpha v beta3 represent an essential signaling complex in the bone-resorbing osteoclast, and, therefore, each is a candidate therapeutic target...
  87. ncbi request reprint Integrin modulation and signaling in leukocyte adhesion and migration
    David M Rose
    Department of Medicine, University of California, and VA Healthcare System, San Diego, CA, USA
    Immunol Rev 218:126-34. 2007
    ..We describe mechanisms through which alpha4beta1 integrin signaling regulates appropriate Rac activation to drive leukocyte migration...
  88. pmc Neurite outgrowth on a fibronectin isoform expressed during peripheral nerve regeneration is mediated by the interaction of paxillin with alpha4beta1 integrins
    Mariette Vogelezang
    Dept of Pathology, University of Cambridge, Cambridge, UK
    BMC Neurosci 8:44. 2007
    ..Here we use PC12 cells to examine the role of the interaction between paxillin and the alpha4 integrin cytoplasmic domain in neurite outgrowth...