Research Topics
Genomes and Genes | S L ForsburgSummaryAffiliation: The Salk Institute Country: USA Publications
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Publications
RNAi hushes heterochromatinJulie M Bailis
Molecular and Cellular Biology Laboratory, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
Genome Biol 3:REVIEWS1035. 2002..Recent work in the fission yeast Schizosaccharomyces pombe reveals that RNA is also an integral component of silent heterochromatin, providing a new perspective on how heterochromatin is organized and maintained in eukaryotic cells...
Mitotic replication initiation proteins are not required for pre-meiotic S phaseS L Forsburg
Molecular Biology and Virology Laboratory, The Salk Institute for Biological Studies, La Jolla, California, USA
Nat Genet 25:263-8. 2000..Our results indicate that the process of replication initiation and checkpoint control may be fundamentally different in mitotic and meiotic cells...- Only connect: linking meiotic DNA replication to chromosome dynamicsSusan L Forsburg
Molecular and Cell Biology Laboratory, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
Mol Cell 9:703-11. 2002..Thus, chromosome dynamics in meiosis are significantly different than in mitotic cells. This review analyzes unique features of meiotic DNA replication and describes how it affects subsequent recombination and chromosome segregation... - Reduced dosage of a single fission yeast MCM protein causes genetic instability and S phase delayD T Liang
Department of Biology, University of California, San Diego CA 92093, USA
J Cell Sci 112:559-67. 1999..Thus, reduced dosage of MCM proteins allows replication initiation, but is insufficient for completion of S phase and cell cycle progression... - Fission yeast enters a joyful new eraEliana B Gómez
Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
Genome Biol 3:REPORTS4017. 2002 
Eukaryotic MCM proteins: beyond replication initiationSusan L Forsburg
Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
Microbiol Mol Biol Rev 68:109-31. 2004..These experiments indicate that the MCMs are central players in many aspects of genome stability...- Mutational analysis of Cdc19p, a Schizosaccharomyces pombe MCM proteinS L Forsburg
Molecular Biology and Virology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
Genetics 147:1025-41. 1997..Not surprisingly, conserved residues in the MCM homology domain are required for protein function, but some residues outside the core homology domain are dispensable... - General purpose tagging vectors for fission yeastS L Forsburg
Molecular Biology and Virology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
Gene 191:191-5. 1997..Using the Cdc19p protein as a test case, we have demonstrated that these plasmids can express functional tagged proteins in the fission yeast cell... - The art and design of genetic screens: yeastS L Forsburg
Molecular and Cell Biology Laboratory, The Salk Institute, 10010 N Torrey Pines Road, La Jolla, California 92037, USA
Nat Rev Genet 2:659-68. 2001..Classical yeast genetics remains an essential element in discovering and characterizing the genes that make up a eukaryotic cell... - Characterization of Schizosaccharomyces pombe mcm7(+) and cdc23(+) (MCM10) and interactions with replication checkpointsD T Liang
Department of Biology, University of California, San Diego, 92093, USA
Genetics 159:471-86. 2001..Mcm7p is a nuclear protein throughout the cell cycle, and its localization is dependent on the other MCM proteins. Our data suggest that the Mcm3p-Mcm5p dimer interacts with the Mcm4p-Mcm6p-Mcm7p core complex through Mcm7p... - Schizosaccharomyces pombe Hsk1p is a potential cds1p target required for genome integrityH A Snaith
Molecular Biology and Virology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037 1099, USA
Mol Cell Biol 20:7922-32. 2000..These results indicate that the Hsk1p kinase is a potential target of Cds1p regulation and that its activity is required after replication initiation for normal mitosis... - Schizosaccharomyces pombe Mcm3p, an essential nuclear protein, associates tightly with Nda4p (Mcm5p)D A Sherman
Molecular Biology and Virology Laboratory, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
Nucleic Acids Res 26:3955-60. 1998..Mcm3p binds particularly tightly to Nda4p (Mcm5p), but is loosely associated with the other Schizosaccharomyces pombe MCM proteins. Thus, Mcm3p is a peripheral MCM subunit... - Isolation of an essential Schizosaccharomyces pombe gene, prp31(+), that links splicing and meiosisD T Bishop
Molecular Biology and Virology Laboratory, The Salk Institute, La Jolla, CA 92037, USA
Nucleic Acids Res 28:2214-20. 2000..Prp31p is closely related to human and budding yeast PRP31 homologs and is likely to function as a general splicing factor in both vegetative growth and sexual differentiation... - Multiple domains of fission yeast Cdc19p (MCM2) are required for its association with the core MCM complexD A Sherman
Molecular Biology and Virology Laboratory, The Salk Institute, La Jolla, California 92037, USA
Mol Biol Cell 9:1833-45. 1998..Using a panel of Cdc19p mutants, we find that multiple domains of Cdc19p are required for MCM binding. These studies indicate that MCM complexes in fission yeast have distinct substructures, which may be relevant for function... - Nuclear localization of Schizosaccharomyces pombe Mcm2/Cdc19p requires MCM complex assemblyS G Pasion
The Salk Institute for Biological Studies, Molecular Biology and Virology Laboratory, La Jolla, California 92037, USA
Mol Biol Cell 10:4043-57. 1999..We suggest that coupling complex assembly to nuclear targeting and retention ensures that only intact heterohexameric MCM complexes remain nuclear... - Rereplication phenomenon in fission yeast requires MCM proteins and other S phase genesH A Snaith
The Salk Institute for Biological Studies, La Jolla, California 92037 1099, USA
Genetics 152:839-51. 1999..Thus rereplication in fission yeast mimics the DNA synthesis of normal S phase, and the inability to rereplicate provides an excellent assay for novel S-phase mutants... - Identification of a second myosin-II in Schizosaccharomyces pombe: Myp2p is conditionally required for cytokinesisM Bezanilla
Structural Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
Mol Biol Cell 8:2693-705. 1997.... - Fission yeast pak1+ encodes a protein kinase that interacts with Cdc42p and is involved in the control of cell polarity and matingS Ottilie
Molecular Biology and Virology Laboratory, Salk Institute, San Diego, CA 92186, USA
EMBO J 14:5908-19. 1995..These results indicate that the Pak1 protein is likely to be an effector for Cdc42p or a related GTPase, and suggest that Pak1p is involved in the maintenance of cell polarity and in mating... - Isolation and characterization of the Pin1/Ess1p homologue in Schizosaccharomyces pombeH K Huang
Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
J Cell Sci 114:3779-88. 2001..Our genetic data also suggest that Pin1p might function as a positive regulator of Cdc25p and Wee1p... - Meiotic S-phase damage activates recombination without checkpoint arrestDaniel G Pankratz
Molecular and Cell Biology Laboratory, The Salk Institute, La Jolla, CA 92037, USA
Mol Biol Cell 16:1651-60. 2005..Our results suggest that fission yeast meiosis is exceptionally tolerant of DNA damage, and that some forms of spontaneous S-phase damage can be repaired by recombination without activating checkpoint arrest... - Hsk1-Dfp1 is required for heterochromatin-mediated cohesion at centromeresJulie M Bailis
Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
Nat Cell Biol 5:1111-6. 2003..This role for Hsk1-Dfp1 is separable from its replication initiation activity, providing a temporal link between S phase and centromere cohesion that is mediated by heterochromatin... - Schizosaccharomyces pombe histone acetyltransferase Mst1 (KAT5) is an essential protein required for damage response and chromosome segregationEliana B Gómez
Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 90089 2910, USA
Genetics 179:757-71. 2008..mst1 mutant sensitivity to hydroxyurea suggests a defect in recovery following HU arrest. We conclude that Mst1 plays essential roles in maintenance of genome stability and recovery from DNA damage... - It's all in the timing: linking S phase to chromatin structure and chromosome dynamicsJulie M Bailis
Molecular and Cell Biology Laboratory, The Salk Institute, La Jolla, California, USA
Cell Cycle 2:303-6. 2003..Intriguingly, recent studies indicate that these processes are linked to S phase by temporal mechanisms as well as by replication-dependent activities... - A screen for Schizosaccharomyces pombe mutants defective in rereplication identifies new alleles of rad4+, cut9+ and psf2+Eliana B Gómez
Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
Genetics 169:77-89. 2005..Other genes identified are likely to play general roles in gene expression and protein localization... - Schizosaccharomyces pombe replication protein Cdc45/Sna41 requires Hsk1/Cdc7 and Rad4/Cut5 for chromatin bindingWilliam P Dolan
MCBL, The Salk Institute, 10010 N Torrey Pines Road, La Jolla, CA 92037, USA
Chromosoma 113:145-56. 2004..In summary, Cdc45p chromatin binding requires an intact pre-replicative complex as well as signaling from both the Dbf4-dependent kinase and cyclin-dependent kinases... - S phase assembly of centromeric heterochromatin and cohesionJulie M Bailis
Molecular and Cell Biology Laboratory, The Salk Institute, La Jolla, California 92037, USA
Cell Cycle 3:416-8. 2004..This defines a temporal connection between S phase, heterochromatin and cohesion that is independent of replication fork passage... - The Schizosaccharomyces pombe aurora-related kinase Ark1 interacts with the inner centromere protein Pic1 and mediates chromosome segregation and cytokinesisJoel D Leverson
Molecular and Cell Biology Laboratory, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037
Mol Biol Cell 13:1132-43. 2002..We also provide evidence that the dominant-negative effect of Ark1-K147R requires Pic1p binding, indicating that the formation of Ark1p-Pic1p complexes is required for the execution of cytokinesis... - Suppressors of Bir1p (Survivin) identify roles for the chromosomal passenger protein Pic1p (INCENP) and the replication initiation factor Psf2p in chromosome segregationHan Kuei Huang
Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
Mol Cell Biol 25:9000-15. 2005.... - Different phenotypes in vivo are associated with ATPase motif mutations in Schizosaccharomyces pombe minichromosome maintenance proteinsEliana B Gómez
Molecular and Cell Biology Laboratory, The Salk Institute, La Jolla, California 92037, USA
Genetics 160:1305-18. 2002..We conclude that the role of ATP binding and hydrolysis is different for different MCM subunits... - Ibp1p, a novel Cdc25-related phosphatase, suppresses Schizosaccharomyces pombe hsk1 ( cdc7)Hilary A Snaith
The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037 1099, USA
Curr Genet 44:38-48. 2003..Ibp1 is not required for viability, suggesting it may be a non-essential regulator of DNA replication or chromosome structure during S phase... - Schizosaccharomyces pombe Rdh54 (TID1) acts with Rhp54 (RAD54) to repair meiotic double-strand breaksMichael G Catlett
Molecular and Cell Biology Laboratory, The Salk Institute, La Jolla, California 92037, USA
Mol Biol Cell 14:4707-20. 2003..pombe meiosis does not result in checkpoint arrest... - Efficient labeling of fission yeast Schizosaccharomyces pombe with thymidine and BUdRJeffrey A Hodson
Molecular and Cell Biology Laboratory, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
Nucleic Acids Res 31:e134. 2003..This strain provides a valuable tool for direct study of DNA replication in S.pombe... - Schizosaccharomyces pombe Rad4/Cut5 protein modification and chromatin binding changes in DNA damageRania Siam
Molecular and Cell Biology Laboratory, The Salk Institute, La Jolla, California, USA
DNA Cell Biol 26:565-75. 2007..In response to replication arrest and DNA damage, the protein is modified in a Rad3-dependent manner. These data indicate that Rad4 undergoes diverse forms of regulation that are distinct in both DNA replication and checkpoint response... - Growth and manipulation of S. pombeSusan L Forsburg
The Salk Institute for Biological Studies, La Jolla, California, USA
Curr Protoc Mol Biol . 2003..pombe, particularly its unwillingness to be a diploid in normal growth conditions. Similarly, the different cell shape and growth behavior of S. pombe require different approaches to basic cell cycle analysis... - Introduction of DNA into S. pombe cellsSusan L Forsburg
The Salk Institute for Biological Studies, La Jolla, California, USA
Curr Protoc Mol Biol . 2003..Protoplasts prepared as for transformation can also be induced to fuse with each other and undergo karyogamy, which provides a means of mating sterile strains... - Analysis of the fission yeast Schizosaccharomyces pombe cell cycleEliana B Gómez
Molecular and Cell Biology Laboratory, The Salk Institute for Biological Sciences, La Jolla, CA, USA
Methods Mol Biol 241:93-111. 2004 - Conserved locus-specific silencing functions of Schizosaccharomyces pombe sir2+Lisa L Freeman-Cook
Division of Biological Sciences, Section of Molecular Biology and UCSD Center for Cancer Research, University of California, San Diego, 92093 0347, USA
Genetics 169:1243-60. 2005..These results also suggest that, despite differences in most of the other molecules required, the two distantly related yeast species share a mechanism for targeting Sir2p homologs to silent chromatin... - MCM proteins: DNA damage, mutagenesis and repairJulie M Bailis
Molecular and Cell Biology Laboratory, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
Curr Opin Genet Dev 14:17-21. 2004..The role of the MCM complex in genome integrity reflects its activity both at active replication forks and away from forks... - Choosing and using Schizosaccharomyces pombe plasmidsRania Siam
Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, 10010 N. Torrey Pines Rd, La Jolla, CA 92037, USA
Methods 33:189-98. 2004..We will also discuss vectors with epitope tags and how these can be used to modify episomal or endogenous gene sequences. Considerations for choosing and using a plasmid are presented and specialized methods are described... - S. pombe strain maintenance and mediaSusan L Forsburg
Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California, USA
Curr Protoc Mol Biol . 2003..The optimal media for S. pombe is somewhat different than that employed for S. cerevisiae, although budding yeast media can be used if necessary. Good sterile technique is essential to prevent contamination by airborne molds or bacteria... - Overview of Schizosaccharomyces pombeSusan L Forsburg
The Salk Institute for Biological Studies, La Jolla, California, USA
Curr Protoc Mol Biol . 2003..Other areas of study are also expanding. Conceptually similar genetic tools are available in both systems, and both organisms have completely sequenced genomes... - Schizosaccharomyces pombe mst2+ encodes a MYST family histone acetyltransferase that negatively regulates telomere silencingEliana B Gómez
Molecular and Computational Biology Section, University of Southern California, Los Angeles, 90089 2910, USA
Mol Cell Biol 25:8887-903. 2005..Although telomere length is normal, meiosis is aberrant in Deltamst2 diploid homozygote mutants, suggesting that telomeric histone acetylation contributes to normal meiotic progression... - Minichromosome maintenance proteins interact with checkpoint and recombination proteins to promote s-phase genome stabilityJulie M Bailis
Molecular and Computational Biology Section, University of Southern California, 1050 Childs Way RRI 201B, Los Angeles, CA 90089 2910, USA
Mol Cell Biol 28:1724-38. 2008.... - Eukaryotic DNA replication in a chromatin contextAngel P Tabancay
Molecular and Computational Biology Section University of Southern California Los Angeles, California 90089, USA
Curr Top Dev Biol 76:129-84. 2006..Thus, in this chapter, we seek to bring together the studies of replication initiation and the studies of chromatin into a single holistic narrative... 
The MCM helicase: linking checkpoints to the replication forkSusan L Forsburg
Molecular and Computational Biology Section, University of Southern California, 1050 Childs Way MCB 201B, Los Angeles, CA 90089 2910, USA
Biochem Soc Trans 36:114-9. 2008....- The yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe: models for cell biology researchSusan L Forsburg
University of Southern California, Los Angeles, CA, USA
Gravit Space Biol Bull 18:3-9. 2005..Each provides unique tools for understanding environmental effects on cellular systems... - Basic methods for fission yeastSusan L Forsburg
Program in Molecular and Computational Biology, University of Southern California, 1050 Child s Way MCB201, Los Angeles, CA 90089 2910, USA
Yeast 23:173-83. 2006..Here, we present basic methods sufficient for a beginner in this system to carry out most required manipulations for genetic analysis or molecular biology...