Research Topics
Species | Alan FlakeSummaryAffiliation: The Children's Hospital of Philadelphia Country: USA Publications
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Publications
Chorioamniotic membrane separation following open fetal surgery: pregnancy outcomeR Douglas Wilson
Department of Surgery, Center for Fetal Diagnosis and Treatment, Children's Hospital of Philadelphia, University of Pennsylvania, Philadelphia, PA, USA
Fetal Diagn Ther 18:314-20. 2003..3) Delivery prior to 33 weeks gestation for MMC groups was 12/43 (28%) with 3 NND (7%). (4) Elective delivery at 36-37 weeks gestation was possible for 43% of the fetal surgery population...- In utero hematopoietic stem cell transplantation. A status reportA W Flake
Department of Surgery, Children s Hospital of Philadelphia, PA 19104, USA
JAMA 278:932-7. 1997.... 
Surgery in the human fetus: the futureAlan W Flake
Department of Surgery and the Center for Fetal Diagnosis and Treatment, Children s Hospital of Philadelphia, PA 19104, USA
J Physiol 547:45-51. 2003....- The conceptual application of systems theory to stem cell biology: a matter of contextAlan W Flake
The Children s Institute for Surgical Science, Children s Hospital of Philadelphia, Philadelphia, PA 19104 4318, USA
Blood Cells Mol Dis 32:58-64. 2004..Although the complexity of the system is beyond current mathematical modeling, the conceptual framework of systems theory can provide context to multiple controversies in stem cell biology... - Genetic therapies for the fetusAlan W Flake
Center for Fetal Diagnosis and Therapy, The Children's Institute for Surgical Science, Children's Hospital of Philadelphia, Pennsylvania 19104, USA
Clin Obstet Gynecol 45:684-96; discussion 730-2. 2002 - In utero stem cell transplantationAlan W Flake
Surgery and Obstetrics and Gynecology, University of Pennsylvania, USA
Best Pract Res Clin Obstet Gynaecol 18:941-58. 2004..This chapter presents the recognized barriers to engraftment in the fetus and discusses promising experimental strategies to overcome these barriers... - In utero stem cell transplantation for the treatment of genetic diseasesA W Flake
Department of Surgery, Children s Hospital of Philadelphia, PA 19104, USA
Schweiz Med Wochenschr 129:1733-9. 1999..In the near future, advances in our understanding of stem cell biology, developmental ontogeny, and gene therapy may allow prenatal stem cell and gene therapy to achieve their full potential... - The current status and future potential of fetal intervention: image is everythingA W Flake
The Center for Fetal Diagnosis and Treatment, Children s Hospital of Philadelphia, PA 19104 4399, USA
Comput Med Imaging Graph 23:51-7. 1999..In the future improvements in imaging and non-invasive technology will drive further expansion of indications and therapeutic options for fetal intervention... - In utero transplantation for thalassemiaA W Flake
Children s Institute of Surgical Science, Children s Hospital of Philadelphia, Pennsylvania 19104, USA
Ann N Y Acad Sci 850:300-11. 1998..Prenatal strategies involving multiple transplants, donor-specific tolerance induction, and postnatal same-donor transplants should be considered... - Sacrococcygeal teratoma: prenatal assessment, fetal intervention, and outcomeHolly L Hedrick
Center for Fetal Diagnosis and Treatment, The Children's Hospital of Philadelphia, Philadelphia, PA 19104-4399, USA
J Pediatr Surg 39:430-8; discussion 430-8. 2004..Amnioreduction, cyst aspiration, and surgical debulking are potentially life-saving interventions... - Reproductive outcomes after pregnancy complicated by maternal-fetal surgeryR Douglas Wilson
Pediatric Surgery, The Children's Hospital of Philadelphia, Philadelphia, PA, USA
Am J Obstet Gynecol 191:1430-6. 2004..The uterine rupture rate is similar to the rupture rate after "classical" cesarean section (4%-9%)... - The EXIT procedure: experience and outcome in 31 casesSarah Bouchard
Division of Pediatric General, The Center for Fetal Diagnosis and Treatment, The Children's Hospital of Philadelphia, Philadelphia, PA, USA
J Pediatr Surg 37:418-26. 2002.... - Transduction of satellite cells after prenatal intramuscular administration of lentiviral vectorsTippi C MacKenzie
The Children's Institute for Surgical Science, Children's Hospital of Philadelphia, Philadelphia, 3615 Civic Center Blvd, Philadelphia, PA 19104-4318, USA
J Gene Med 7:50-8. 2005..These findings may have important implications for gene therapy strategies directed toward muscular dystrophy... - Management of congenital lung lesionsN Scott Adzick
Center for Fetal Diagnosis and Treatment, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA
Semin Pediatr Surg 12:10-6. 2003..The finding that fetuses with hydrops are at very high risk for fetal or neonatal death has led to successful fetal thoracoamniotic shunt placement or fetal surgical resection... - Persistent expression of hF.IX After tolerance induction by in utero or neonatal administration of AAV-1-F.IX in hemophilia B miceDenise E Sabatino
Department of Hematology, Children s Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA
Mol Ther 15:1677-85. 2007..IX expression. This supports the concept of a narrow "window of opportunity" for tolerance induction... 
Congenital high airway obstruction syndrome: MR/US findings, effect on management, and outcomeAndrew Mong
Department of Radiology, Children s Hospital of Philadelphia, Philadelphia, PA 19104, USA
Pediatr Radiol 38:1171-9. 2008..Prompt airway intervention at delivery after accurate prenatal diagnosis may allow survival of this otherwise fatal condition...- Liver position and lung-to-head ratio for prediction of extracorporeal membrane oxygenation and survival in isolated left congenital diaphragmatic herniaHolly L Hedrick
Center for Fetal Diagnosis and Treatment, Children s Hospital of Philadelphia, Philadelphia, PA, USA
Am J Obstet Gynecol 197:422.e1-4. 2007..The purpose of this study was to determine the ability of liver position and lung-to-head ratio to predict outcome in isolated left congenital diaphragmatic hernia... - Abnormal umbilical cord Doppler sonograms may predict impending demise in fetuses with sacrococcygeal teratoma. A report of two casesOluyinka O Olutoye
Center for Fetal Diagnosis and Treatment, Children's Hospital of Philadelphia, Philadelphia, Pa. 19104, USA
Fetal Diagn Ther 19:35-9. 2004..In such fetuses, depending on the gestational age, abnormalities in umbilical artery waveform should be considered indications for early delivery or in utero intervention to prevent fetal demise... - Epinephrine inhibits tracheal occlusion induced lung growth in fetal sheepYoshihiro Kitano
The Children's Institute for Surgical Science, The Children's Hospital of Philadelphia, Philadelphia, PA 19104-4399, USA
Fetal Diagn Ther 18:333-7. 2003..Drugs affecting lung fluid secretion may have a major impact on TO-induced lung growth... - Severe pulmonary hypoplasia associated with giant cervical teratomasKenneth W Liechty
Center for Fetal Diagnosis and Treatment, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA
J Pediatr Surg 41:230-3. 2006..Despite obtaining airway control, a subset of these patients will die because of pulmonary hypoplasia. When counseling patients with large cervical masses it is important to discuss potential pulmonary hypoplasia in these patients... - Abnormal umbilical cord Dopplers may predict impending demise in fetuses with sacrococcygeal teratoma. A report of 2 casesOluyinka O Olutoye
The Center for Fetal Diagnosis and Treatment, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA
Fetal Diagn Ther 18:428-31. 2003..In such fetuses, depending on the gestational age, abnormalities in umbilical artery waveform should be considered indications for early delivery or in utero intervention to prevent fetal demise... - Congenital high airway obstruction syndrome: natural history and managementFoong-Yen Lim
Center for Fetal Diagnosis and Treatment, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA
J Pediatr Surg 38:940-5. 2003..The EXIT procedure offers the potential for salvage of this otherwise lethal condition. Hydrops may be well tolerated prenatally for weeks with potential resolution if airway fistulization is present... - Fetal tracheal occlusion for severe congenital diaphragmatic hernia in humans: a morphometric study of lung parenchyma and muscularization of pulmonary arteriolesEnrico Danzer
The Center for Fetal Diagnosis and Treatment, The Children s Hospital of Philadelphia, Philadelphia, PA 19104 4318, USA
J Pediatr Surg 43:1767-75. 2008..The aim of this study is to examine structure of lung parenchyma (Pp) and muscularization of pulmonary arterioles (PAs) in human fetuses that underwent tracheal occlusion (TO) therapy for severe congenital diaphragmatic hernia (CDH)... - Emergent excision of a prenatally diagnosed sacrococcygeal teratomaKha M Tran
Department of Anesthesiology and Critical Care Medicine, The Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
Paediatr Anaesth 18:431-4. 2008..We present a case of prenatally diagnosed SCT and address the evaluation, anesthetic considerations, and mechanisms needed to care for this high risk population... - Sacrococcygeal teratomas: prenatal surveillance, growth and pregnancy outcomeR Douglas Wilson
Fetal Heart Program, The Center for Fetal Diagnosis and Treatment, The Children s Hospital of Philadelphia, Philadelphia, PA 19104, USA
Fetal Diagn Ther 25:15-20. 2009..Prenatal surveillance and growth characteristics are evaluated in a cohort of fetuses with sacrococcygeal teratomas (SCT) as part of risk assessment... - Successful revision of portoenterostomy in an infant with biliary atresiaBarbara A Haber
Division of Gastroenterology and Nutrition, The Children s Hospital of Philadelphia, Philadelphia, PA 19104, USA
J Pediatr Surg 41:e1-3. 2006..At 14 months of age, the child is anicteric and growing well. In this case, successful revision of hepatoportoenterostomy averted the need for liver transplantation... - Predicting the severity of congenital high airway obstruction syndromeJessica L Roybal
Center for Fetal Diagnosis and Treatment, Children s Hospital of Philadelphia, PA 19104 4318, USA
J Pediatr Surg 45:1633-9. 2010..In our relatively large series of CHAOS patients, we have observed a spectrum of clinical severity. The aim of this study was to identify the prenatal characteristics of CHAOS predictive of a milder postnatal course... - Apoptotic cell-mediated immunoregulation of dendritic cells does not require iC3b opsonizationEdward M Behrens
Laboratory of Dendritic Cell Biology, Division of Rheumatology, Joseph Stokes, Jr Research Institute, Children s Hospital of Philadelphia, Philadelphia, PA 19104 4318, USA
J Immunol 181:3018-26. 2008..We conclude that the apoptotic cell receptor system on DCs is sufficiently redundant such that the absence of CR3 engagement does not significantly affect the normal anti-inflammatory processing of apoptotic cells... - Prenatal glucocorticoids and exogenous surfactant therapy improve respiratory function in lambs with severe diaphragmatic hernia following fetal tracheal occlusionMarcus G Davey
The Children s Institute for Surgical Science and the Center for Fetal Diagnosis and Treatment, The Children s Hospital of Philadelphia, PA 19104, USA
Pediatr Res 60:131-5. 2006..GC or surfactant therapy alone significantly improved respiratory gas exchange, Cl, and ventilatory efficiency index. Total lung capacity was normalized only in DH/TO lambs that received both GC and S... - Congenital pulmonary lymphangiectasis sequence: a rare, heterogeneous, and lethal etiology for prenatal pleural effusionR Douglas Wilson
The Center for Fetal Diagnosis and Treatment, Department of Surgery at The Children s Hospital of Philadelphia University of Pennsylvania School of Medicine, Philadelphia, PA 19104 4399, USA
Prenat Diagn 26:1058-61. 2006..Autopsy-identified CPL... - Does a myelomeningocele sac compared to no sac result in decreased postnatal leg function following maternal fetal surgery for spina bifida aperta?R Douglas Wilson
Department of Surgery and Obstetrics and Gynecology, Center for Fetal Diagnosis and Treatment, Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 4399, USA
Fetal Diagn Ther 22:348-51. 2007..A fetus with large sac S1 myelomeningocele (MMC) but bilateral talipes prompted the question, 'Does the presence or size of an MMC sac affect postnatal leg function?'.. - Maternal alloantibodies induce a postnatal immune response that limits engraftment following in utero hematopoietic cell transplantation in miceDemetri J Merianos
Children s Center for Fetal Research, Children s Hospital of Philadelphia, Philadelphia, Pennsylvania, USA
J Clin Invest 119:2590-600. 2009..This study resolves the apparent contradiction of induction of an adaptive immune response in the pre-immune fetus and confirms the potential of actively acquired tolerance to facilitate prenatal therapeutic applications... - Effect of maternal betamethasone administration on prenatal congenital cystic adenomatoid malformation growth and fetal survivalWilliam H Peranteau
Center for Fetal Diagnosis and Treatment, Children s Hospital of Philadelphia and University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
Fetal Diagn Ther 22:365-71. 2007..To evaluate the effect of prenatal steroid treatment on the growth of congenital cystic adenomatoid malformations (CCAM) and survival in affected fetuses not amenable to other percutaneous ultrasound-guided prenatal interventions... - In utero meconium passage in fetuses and newborns with myelomeningoceleEnrico Danzer
The Center for Fetal Diagnosis and Treatment, The Children s Hospital of Philadelphia, and the University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 4318, USA
J Neurosurg Pediatr 3:141-6. 2009..The authors retrospectively investigated whether midgestational fetal myelomeningocele (fMMC) repair alters intrauterine meconium exposure... - Repair of indirect inguinal hernias by "laparoscopic joining of the edges"Pablo Laje
Department of Pediatric Surgery, Children s Hospital of Philadelphia, Philadelphia, PA, USA
Surg Laparosc Endosc Percutan Tech 18:280-2. 2008..To evaluate a new laparoscopic technique for the treatment of indirect inguinal hernias... - Stem cell and genetic therapies for the fetusJessica L Roybal
Children s Center for Fetal Research, Children s Hospital of Philadelphia, PA, USA
Semin Fetal Neonatal Med 15:46-51. 2010..Here we review the current status and future potential of prenatal cellular and genetic therapy... - In utero hematopoietic stem cell transplantation: progress toward clinical applicationDemetri Merianos
Children s Center for Fetal Research, Children s Hospital of Philadelphia, Philadelphia, Pennsylvania, USA
Biol Blood Marrow Transplant 14:729-40. 2008..Because donor specific tolerance induction requires relatively minimal engraftment, this strategy may hold the key to broad clinical application of IUHCT in the near future... - Pulmonary vascular malformationsKenneth W Liechty
Center for Fetal Research, The Children s Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA
Semin Pediatr Surg 17:9-16. 2008..This paper will review the components of these lesions, their associated anomalies, the diagnosis and natural history, and finally, current concepts in the management of pulmonary vascular malformations... - Cystic adenomatoid malformation of the lung: review of genetics, prenatal diagnosis, and in utero treatmentR Douglas Wilson
Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 4399, USA
Am J Med Genet A 140:151-5. 2006..In the appropriate situation, this maternal fetal surgery approach for CCAM is life-saving for the affected fetus with acceptable maternal morbidity risks in the present and future pregnancies... - AAV-mediated factor IX gene transfer to skeletal muscle in patients with severe hemophilia BCatherine S Manno
Department of Pediatrics, University of Pennsylvania and The Children s Hospital of Philadelphia, PA, 19104, USA
Blood 101:2963-72. 2003.... - Prenatal glucocorticoids improve lung morphology and partially restores surfactant mRNA expression in lambs with diaphragmatic hernia undergoing fetal tracheal occlusionMarcus G Davey
The Children s Institute for Surgical Science and the Center for Fetal Diagnosis and Treatment, The Children s Hospital of Philadelphia, Philadelphia, Pennsylvania, USA
Pediatr Pulmonol 41:1188-96. 2006..We hypothesize that a GC-induced reduction in lung liquid volume during TO contributes, in part, to the observed increase in AE2 cell density and SPmRNA expression... - Temporary tracheal occlusion in fetal sheep with lung hypoplasia does not improve postnatal lung functionMarcus G Davey
Children s Institute for Surgical Science and Center for Fetal Diagnosis and Treatment, Children s Hospital of Philadelphia, Philadelphia, Pennsylvania 19104 4399, USA
J Appl Physiol 94:1054-62. 2003..Lung compliance remained low in DH/TO+R lambs, most likely as a consequence of the persistent increase in alveolar wall thickness in these animals... - High-level allogeneic chimerism achieved by prenatal tolerance induction and postnatal nonmyeloablative bone marrow transplantationWilliam H Peranteau
Children's Institute for Surgical Science, The Children's Hospital of Philadelphia, 34th Street and Civic Center Boulevard, Philadelphia, PA 19104, USA
Blood 100:2225-34. 2002..This study supports the concept of prenatal tolerance induction to facilitate nonmyeloablative postnatal strategies for cellular therapy. If clinically applicable, such an approach could dramatically expand the application of IUHSCT... - Thoracoamniotic shunts: fetal treatment of pleural effusions and congenital cystic adenomatoid malformationsR Douglas Wilson
Department of Surgery, Center for Fetal Diagnosis and Treatment at the Children s Hospital of Philadelphia, University of Pennsylvania, Philadelphia, PA 19104 4399, USA
Fetal Diagn Ther 19:413-20. 2004.... - Right congenital diaphragmatic hernia: Prenatal assessment and outcomeHolly L Hedrick
Center for Fetal Diagnosis and Treatment, The Children's Hospital of Philadelphia, Philadelphia, PA 19104-4399, USA
J Pediatr Surg 39:319-23; discussion 319-23. 2004.... - Fetal myelomeningocele repair: short-term clinical outcomesMark P Johnson
Center for Fetal Diagnosis and Treatment, Children s Hospital of Philadelphia and The University of Pennsylvania School of Medicine, Philadephia, PA 19104, USA
Am J Obstet Gynecol 189:482-7. 2003..The study was undertaken to evaluate short-term clinical outcomes with antenatal myelomeningocele (MMC) repair... - Acute cardiovascular effects of fetal surgery in the humanJack Rychik
Center for Fetal Diagnosis and Treatment and the Fetal Heart Program, The Children s Hospital of Philadelphia, 34th St and Civic Center Blvd, Philadelphia, PA 19104, USA
Circulation 110:1549-56. 2004..The indications for fetal surgery continue to expand, yet little is known about the acute sequelae of fetal surgery on the human cardiovascular system... - Surfactant protein expression is increased in the ipsilateral but not contralateral lungs of fetal sheep with left-sided diaphragmatic herniaMarcus G Davey
Children s Institute for Surgical Science and Center for Fetal Diagnosis and Treatment, Children s Hospital of Philadelphia, Abramson 1112 D, 3615 Civic Center Blvd, Philadelphia, PA 19104, USA
Pediatr Pulmonol 39:359-67. 2005..Furthermore, AE2 cell density is increased in DH, suggesting that the increase in SP mRNA and protein levels is due to increases AE2 cell number. Our data further support the premise that fetal lung hypoplasia favors an AE2 phenotype... - The ex utero intrapartum therapy procedure for high-risk fetal lung lesionsHolly L Hedrick
The Center for Fetal Diagnosis and Treatment, The Children s Hospital of Philadelphia, Philadelphia, PA 19104 4399, USA
J Pediatr Surg 40:1038-43; discussion 1044. 2005..Indications for the ex utero intrapartum therapy (EXIT) procedure have expanded to include any fetal anomaly in which resuscitation of the neonate may be compromised... - A randomized trial of prenatal versus postnatal repair of myelomeningoceleN Scott Adzick
Children s Hospital of Philadelphia, Philadelphia, PA 19104, USA
N Engl J Med 364:993-1004. 2011..Prenatal repair of myelomeningocele, the most common form of spina bifida, may result in better neurologic function than repair deferred until after delivery. We compared outcomes of in utero repair with standard postnatal repair... - Outcomes after postnatal resection of prenatally diagnosed asymptomatic cystic lung lesionsANTHONY Y TSAI
The Center for Fetal Diagnosis and Treatment, The Children s Hospital of Philadelphia, Philadelphia, PA 19104, USA
J Pediatr Surg 43:513-7. 2008..On the other hand, malignant degeneration, pneumonia, and pneumothorax are known consequences of cystic lung lesions. This study aims to assess the safety of resection for asymptomatic lung lesions that were diagnosed before birth... - Main bronchus occlusion for treatment of congenital diaphragmatic hernia in fetal lambsJean Marc Biard
The Center for Fetal Diagnosis and Treatment at the Children s Hospital of Philadelphia, PA 19104 4399, USA
J Pediatr Surg 43:620-6. 2008.... - Cystic adenomatoid malformations are induced by localized FGF10 overexpression in fetal rat lungSilvia Gonzaga
The Children s Center for Fetal Research, Children s Hospital of Philadelphia, Philadelphia, Pennsylvania 19104 4318, USA
Am J Respir Cell Mol Biol 39:346-55. 2008..These findings support a role for FGF10 in the induction of human CCAM and provide further mechanistic insight into the role of FGF10 in normal and abnormal lung development... - Mixed chimerism following in utero hematopoietic stem cell transplantation in murine models of hemoglobinopathySatoshi Hayashi
The Childrens Institute for Surgical Science, Department of Surgery, The Children's Hospital of Philadelphia, 3516 Civic Center Boulevard, Philadelphia, PA 19104-4399, USA
Exp Hematol 31:176-84. 2003..CONCLUSION: The severity of the hemoglobinopathy being treated and the source of donor cells may be important determinants of success in the treatment of hemoglobinopathy by IUHSCTx... - Reproductive outcomes in subsequent pregnancies after a pregnancy complicated by open maternal-fetal surgery (1996-2007)R Douglas Wilson
Center for Fetal Diagnosis and Treatment, Children s Hospital of Philadelphia, Philadelphia, PA, USA
Am J Obstet Gynecol 203:209.e1-6. 2010..The reproductive and gynecologic outcomes for women after the pregnancy complicated by open maternal-fetal surgery (OMFS) were evaluated... - Correction of murine ADAMTS13 deficiency by hematopoietic progenitor cell-mediated gene therapyPablo Laje
Department of General Surgery, The Children s Hospital of Philadelphia and The University of Pennsylvania Medical Center, Philadelphia, PA 19104, USA
Blood 113:2172-80. 2009..This success may provide the basis for development of a novel therapeutic strategy to cure hereditary TTP in humans... - Haploidentical in utero hematopoietic cell transplantation improves phenotype and can induce tolerance for postnatal same-donor transplants in the canine leukocyte adhesion deficiency modelWilliam H Peranteau
Center for Fetal Research, Children s Hospital of Philadelphia, Philadelphia, Pennsylvania, USA
Biol Blood Marrow Transplant 15:293-305. 2009.... - Prenatal transplantation of cytokine-stimulated marrow improves early chimerism in a resistant strain combination but results in poor long-term engraftmentAimen F Shaaban
Laboratory for Fetal Cellular Therapy, Department of Surgery, University of Wisconsin Medical School, Madison, WI 53792 7375, USA
Exp Hematol 34:1278-87. 2006..To evaluate this hypothesis, we stimulated the donor cells with vascular endothelial growth factor (VEGF) and stem cell factor (SCF) prior to IUHCT in a chimerism-resistant murine strain combination... - CD26 inhibition enhances allogeneic donor-cell homing and engraftment after in utero hematopoietic-cell transplantationWilliam H Peranteau
The Center for Fetal Research, Children's Hospital of Philadelphia, Abramson Research Bldg, Rm 1116B, 3615 Civic Center Blvd, Philadelphia, PA 19104-4318, USA
Blood 108:4268-74. 2006..The resulting increased donor chimerism suggests that CD26 inhibition may in the future be used as a method of increasing donor-specific tolerance following IUHCT... - Structural and functional characterization of bladder smooth muscle in fetal rats with retinoic acid-induced myelomeningoceleEnrico Danzer
The Center for Fetal Research, The Children s Hospital of Philadelphia, The University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 4318, USA
Am J Physiol Renal Physiol 292:F197-206. 2007.... - Evidence for an immune barrier after in utero hematopoietic-cell transplantationWilliam H Peranteau
Center for Fetal Research, Children s Hospital of Philadelphia, PA 19104, USA
Blood 109:1331-3. 2007..In contrast, all congenic recipients maintain stable, long-term, multilineage chimerism. These results strongly support an immune barrier to allogeneic engraftment after IUHCT... - Diagnosis and characterization of fetal sacrococcygeal teratoma with prenatal MRIEnrico Danzer
The Center for Fetal Diagnosis and Treatment, Department of Radiology, The Children's Hospital of Philadelphia, Philadelphia, PA 19104-4399, USA
AJR Am J Roentgenol 187:W350-6. 2006..The additional anatomic resolution provided by MRI resulted in more accurate prenatal counseling and improved preoperative planning for surgical resection... - Fetal liver as a source of autologous progenitor cells for perinatal tissue engineeringAlexander S Krupnick
Harrison's Department of Surgical Research, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania, USA
Tissue Eng 10:723-35. 2004..We have demonstrated that mesenchymal progenitor cells with multilineage potential can be isolated from the fetal liver and have potential utility for autologous tissue engineering... - Targeted gene transfer to fetal rat lung interstitium by ultrasound-guided intrapulmonary injectionTiago Henriques Coelho
The Center for Fetal Research, Children s Hospital of Philadelphia, Philadelphia, Pennsylvania, USA
Mol Ther 15:340-7. 2007..This model system may facilitate in vivo studies of dynamic lung morphogenesis and could provide insight into the efficacy of prenatal gene transfer strategies for treatment of specific lung disorders... - Gene transfer to ocular stem cells by early gestational intraamniotic injection of lentiviral vectorMasayuki Endo
The Children s Center for Fetal Research, Children s Hospital of Philadelphia, Philadelphia, Pennsylvania, USA
Mol Ther 15:579-87. 2007..This model may be useful for the investigation of mechanisms of genetic and/or developmental ocular disease and for the development of prenatal gene therapy for specific ocular disorders... - Long-term transgene expression in cardiac and skeletal muscle following fetal administration of adenoviral or adeno-associated viral vectors in miceSarah Bouchard
Division of Pediatric General, Thoracic, and Fetal Surgery, The Children's Institute for Surgical Science, The Children's Hospital of Philadelphia, Philadelphia, PA, USA
J Gene Med 5:941-50. 2003..The potential for immune response to viral antigens is altered, but not entirely ablated after in utero exposure... - Postnatal chest wall deformities after fetal thoracoamniotic shunting for congenital cystic adenomatoid malformationAziz M Merchant
Center for Fetal Diagnosis and Treatment, Children s Hospital of Philadelphia, and Department of Surgery and Obstetrics Gynecology, University of Pennsylvania, Philadelphia, PA 19104 4399, USA
Fetal Diagn Ther 22:435-9. 2007..Large macrocystic congenital cystic adenomatoid malformations (CCAMs) can be treated with thoracoamniotic (TA) shunting to reduce CCAM volume. Two CCAM fetuses treated with TA shunt had postnatal radiographic rib deformities... - Intradural inclusion cysts following in utero closure of myelomeningocele: clinical implications and follow-up findingsEnrico Danzer
The Center for Fetal Diagnosis and Treatment, The Children s Hospital of Philadelphia and the University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
J Neurosurg Pediatr 2:406-13. 2008..The goal in this study was to evaluate the incidence and clinical implications of the development of cutaneously derived intradural inclusion cysts (ICs) following fetal myelomeningocele (fMMC) closure... - Preliminary investigations into a new method of functional assessment of the fetal heart using a novel application of 'real-time' cardiac magnetic resonance imagingMark A Fogel
Division of Cardiology, Department of Pediatrics, University of Pennsylvania School of Medicine, Children s Hospital of Philadelphia, Philadelphia, PA 19104, USA
Fetal Diagn Ther 20:475-80. 2005..Because of quantitative echocardiographic limitations of fetal ventricular volumes as well as poor windows, we sought to determine if real-time magnetic resonance imaging (MRI) could be used... - A tissue engineering approach for prenatal closure of myelomeningocele: comparison of gelatin sponge and microsphere scaffolds and bioactive protein coatingsMiho Watanabe
Department of Surgery and Children s Center for Fetal Research, Children s Hospital of Philadelphia, Philadelphia, Pennsylvania 19104 4318, USA
Tissue Eng Part A 17:1099-110. 2011..These findings support the potential for the development of a tissue-engineered injectable scaffold that could be applied by ultrasound-guided injection, much earlier and less invasively than sponge or sheet-based composites... - The natural history of prenatally diagnosed conjoined twinsTippi C MacKenzie
Center for Fetal Diagnosis and Treatment, The Children's Hospital of Philadelphia, Philadelphia, PA, USA
J Pediatr Surg 37:303-9. 2002..CONCLUSIONS: Advances in prenatal diagnosis allow detailed, accurate evaluations of conjoined twins. Careful prenatal studies may uncover cases in which emergent separation at birth is lifesaving... - Cystic adenomatoid malformation volume ratio predicts outcome in prenatally diagnosed cystic adenomatoid malformation of the lungTimothy M Crombleholme
Division of General, The Center for Fetal Diagnosis and Treatment at The Children's Hospital of Philadelphia, USA
J Pediatr Surg 37:331-8. 2002..The CVR is a useful sonographic indicator of fetuses at risk for hydrops who require close ultrasound observation and possible fetal intervention... - Influence of congenital heart disease on survival in children with congenital diaphragmatic herniaMeryl S Cohen
Cardiac Center and The Center for Fetal Diagnosis and Treatment, The Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia 19104, USA
J Pediatr 141:25-30. 2002..2 died; 3 of 6 with an LHR >1.2 survived (Fisher exact test, P =.04). CONCLUSION: Heart disease remains a significant risk factor for death in infants with CDH. The LHR helps predict survival in this high-risk group of patients... - Antenatal vitamin A administration attenuates lung hypoplasia by interfering with early instead of late determinants of lung underdevelopment in congenital diaphragmatic herniaMaria J Baptista
Development Unit, Health and Life Research Institute, Health Sciences School, University of Minho, 4710-057 Braga, Portugal
J Pediatr Surg 40:658-65. 2005..This finding may have clinical implications because prenatal diagnosis of human CDH commonly occurs after 16 weeks' gestation when late determinants of lung hypoplasia likely predominate... - Complete allogeneic hematopoietic chimerism achieved by a combined strategy of in utero hematopoietic stem cell transplantation and postnatal donor lymphocyte infusionSatoshi Hayashi
Children's Institute for Surgical Science, The Children's Hospital of Philadelphia, PA 19104-4399, USA
Blood 100:804-12. 2002.... - Fetal heart development in the nitrofen-induced CDH rat model: the role of mechanical and nonmechanical factorsJorge Correia-Pinto
Department of Physiology, Faculty of Medicine, University of Porto, Porto, Portugal
J Pediatr Surg 38:1444-51. 2003..Heart weight predicts lung weight only in early gestational ages... - Fetal therapyMark I Evans
Columbia University College of Physicians and Surgeons, St Luke's Roosevelt Hospital Center, 1000 10th Avenue, New York, NY 10019, USA
Best Pract Res Clin Obstet Gynaecol 16:671-83. 2002.... - Gastroschisis: growth patterns and a proposed prenatal surveillance protocolDenise A Netta
Division of Maternal Fetal Medicine, Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, New Brunswick, NJ, USA
Fetal Diagn Ther 22:352-7. 2007..The growth assessment could be available as a prospective tool to direct an antepartum fetal surveillance protocol... - Stem cell and genetic therapies for the fetusAlan W Flake
The Center for Fetal Diagnosis and Treatment, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA
Semin Pediatr Surg 12:202-8. 2003..Although clinical application is currently limited to a few highly selected disorders that are amenable to cellular therapy, there is reason to believe that a dramatic increase in application will occur in the near future... - Engraftment of bone marrow and fetal liver cells after in utero transplantation in MDX miceTippi C MacKenzie
Children's Institute for Surgical Science, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA
J Pediatr Surg 37:1058-64. 2002..Because muscular dystrophy now can be detected early in gestation, such a strategy may offer a future alternative in the clinical treatment of this disease...
Research Grants
- Fetal Biology and Therapy Training ProgramAlan Flake; Fiscal Year: 2007..Penn Graduate School and IHGT. This request is made for 2 Trainee slots in year 1, 3 Trainee slots in year 2, and 4 Trainee slots each year thereafter. ..
- Strategies for prenatal stem cell transplantationAlan Flake; Fiscal Year: 2007..The ultimate goal of these studies is to develop safe and effective strategies that can be translated to the in utero treatment of a broad range of human genetic hematologic disorders including Sickle Cell Disease and Thalassemia. ..
- Fetal Stromal Progenitor CellsAlan Flake; Fiscal Year: 2006..abstract_text> ..
- Strategies for Prenatal Stem Cell TranplantationAlan Flake; Fiscal Year: 2004..The studies in this proposal are designed to identify and test strategies to increase engraftment after IUHSCTx. This would allow application of this approach to a broad range of prenatally diagnosed genetic disorders. ..
- PRENATAL STRATEGIES FOR TREATMENT OF HEMOGLOBINOPATHIESAlan Flake; Fiscal Year: 2002..abstract_text> ..
- Strategies for prenatal stem cell transplantationAlan W Flake; Fiscal Year: 2010..The ultimate goal of these studies is to develop safe and effective strategies that can be translated to the in utero treatment of a broad range of human genetic hematologic disorders including Sickle Cell Disease and Thalassemia. ..