Research Topics
| Vincent FischettiSummaryAffiliation: The Rockefeller University Country: USA Publications
Research Grants
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Detail Information
Publications
Bacteriophage lytic enzymes: novel anti-infectivesVincent A Fischetti
Laboratory of Bacterial Pathogenesis, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
Trends Microbiol 13:491-6. 2005..Thus, lysins could be an effective anti-infective in an age of mounting antibiotic resistance...- Reinventing phage therapy: are the parts greater than the sum?Vincent A Fischetti
Laboratory of Bacterial Pathogenesis and Immunology, Rockefeller University, New York, New York 10021, USA
Nat Biotechnol 24:1508-11. 2006..Although whole phage continue to generate interest as an alternative to antibiotics, focus is shifting to the use of purified phage components as antibacterial agents... - In vivo acquisition of prophage in Streptococcus pyogenesVincent A Fischetti
Laboratory of Bacterial Pathogenesis and Immunology, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
Trends Microbiol 15:297-300. 2007..This suggests that, through evolution, phage have devised a system whereby progeny phage are induced and transferred to host streptococci at a site where host organisms are more prevalent... 
Synergism between a novel chimeric lysin and oxacillin protects against infection by methicillin-resistant Staphylococcus aureusAnu Daniel
The Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, 1230 York Ave, Box 172, New York, NY 10065, USA
Antimicrob Agents Chemother 54:1603-12. 2010..aureus infections and would allow for the reinstatement of antibiotics shelved because of mounting resistance...- Bacteriophage lysins as effective antibacterialsVincent A Fischetti
Laboratory of Bacterial Pathogenesis, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
Curr Opin Microbiol 11:393-400. 2008..Thus, lysins may be a much needed anti-infective in an age of mounting antibiotic resistance... 
Bacteriophage endolysins: a novel anti-infective to control Gram-positive pathogensVincent A Fischetti
Laboratory of Bacterial Pathogenesis, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
Int J Med Microbiol 300:357-62. 2010..Here we review the current literature showing the effectiveness of these enzymes in controlling a variety of infections...- A bacteriolytic agent that detects and kills Bacillus anthracisRaymond Schuch
Laboratory of Bacteriql Pathogenesis and Immunology, The Rockefeller University, New York, New York 10021, USA
Nature 418:884-9. 2002..The lytic specificity of PlyG was also exploited as part of a rapid method for the identification of B. anthracis. We conclude that PlyG is a tool for the treatment and detection of B. anthracis... - Identification of a broadly active phage lytic enzyme with lethal activity against antibiotic-resistant Enterococcus faecalis and Enterococcus faeciumPauline Yoong
Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, 1230 York Ave, New York, NY 10021, USA
J Bacteriol 186:4808-12. 2004..faecalis and E. faecium (including vancomycin-resistant strains), as well as other human pathogens. We propose its development and use as an alternative therapeutic tool... - A novel secreted endoglycosidase from Enterococcus faecalis with activity on human immunoglobulin G and ribonuclease BMattias Collin
Laboratory of Bacterial Pathogenesis, The Rockefeller University, New York, New York 10021, USA
J Biol Chem 279:22558-70. 2004..faecalis, EndoE, which by two distinct activities hydrolyzes the glycans on RNase B and IgG. Both activities could be important for the molecular pathogenesis and persistence of E. faecalis during human infections... - The secret life of the anthrax agent Bacillus anthracis: bacteriophage-mediated ecological adaptationsRaymond Schuch
The Rockefeller University, New York, NY, USA
PLoS ONE 4:e6532. 2009..The results here demonstrate that during its environmental phase, bacteriophages provide B. anthracis with alternatives to sporulation that involve the activation of soil-survival and endosymbiotic capabilities... - M.SpyI, a DNA methyltransferase encoded on a mefA chimeric element, modifies the genome of Streptococcus pyogenesChad W Euler
Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, Box 172, 1230 York Avenue, New York, NY 10021, USA
J Bacteriol 189:1044-54. 2007..The presence of the SpyI R-M cassette in Phi10394.4 could impart a selective advantage to host strain survival and may provide another explanation for the observed increase in macrolide-resistant streptococci... - First complete genome sequence of two Staphylococcus epidermidis bacteriophagesAnu Daniel
Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, New York, NY 10021, USA
J Bacteriol 189:2086-100. 2007..Until now, no S. epidermidis phage genome sequences have been reported in the literature, and thus this study represents the first complete genomic and molecular description of two S. epidermidis phages... - PlyC: a multimeric bacteriophage lysinDaniel Nelson
Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
Proc Natl Acad Sci U S A 103:10765-70. 2006..Similar to no other proteins in sequence databases, PlyC defines a previously uncharacterized structural family of cell-wall hydrolases... - Detailed genomic analysis of the Wbeta and gamma phages infecting Bacillus anthracis: implications for evolution of environmental fitness and antibiotic resistanceRaymond Schuch
Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, New York, NY 10021, USA
J Bacteriol 188:3037-51. 2006..anthracis life cycle. Initial findings suggest that lysogeny of B. anthracis promotes ecological adaptation, rather than virulence, as with other gram-positive pathogens... - PlyPH, a bacteriolytic enzyme with a broad pH range of activity and lytic action against Bacillus anthracisPauline Yoong
Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
J Bacteriol 188:2711-4. 2006..PlyPH remains active between pH 4 and 10.5, and a single dose rescued a significant percentage of mice infected intraperitoneally with an attenuated B. anthracis strain. We propose PlyPH as a novel therapeutic agent... - The in vitro interaction of Streptococcus pyogenes with human pharyngeal cells induces a phage-encoded extracellular DNaseThomas B Broudy
Department of Bacterial Pathogenesis and Immunology, Rockefeller University, New York, New York 10021, USA
Infect Immun 70:2805-11. 2002..Finally, we speculate on the induction of phage-encoded DNase (Spd1) enhancing the fitness of both bacteria and phage... - Lytic enzyme discovery through multigenomic sequence analysis in Clostridium perfringensJonathan E Schmitz
Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, New York, NY 10065, USA
Appl Microbiol Biotechnol 89:1783-95. 2011..Overall, PlyCM shows potential for development as an enzybiotic agent, demonstrating how expanding genomic databases can serve as rich pools for biotechnologically relevant proteins... - The career of Maclyn McCartyEmil C Gotschlich
Laboratory of Bacterial Pathogenesis and Immunology, Rockefeller University, New York, NY 10021, USA
J Exp Med 201:1699-707. 2005..But McCarty's scientific career was long and fruitful, and his contributions to science were vast. This retrospective offers a look at some of Mac's other notable scientific achievements... - Identifying active phage lysins through functional viral metagenomicsJonathan E Schmitz
Rockefeller University, Laboratory of Bacterial Pathogenesis and Immunology, 1230 York Ave, Box 172, New York, NY 10065, USA
Appl Environ Microbiol 76:7181-7. 2010..Overall, this study represents one of the first functional screens of a viral metagenomic population, and it provides a general approach for characterizing lysins from uncultured phage... - A novel chimeric lysin shows superiority to mupirocin for skin decolonization of methicillin-resistant and -sensitive Staphylococcus aureus strainsMina Pastagia
Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, New York, NY 10065, USA
Antimicrob Agents Chemother 55:738-44. 2011..Our results showed no inhibition of ClyS activity at various antibody titers. These data demonstrate the potential of developing ClyS as a novel class of topical antimicrobial agents specific to staphylococcus... - Removal of group B streptococci colonizing the vagina and oropharynx of mice with a bacteriophage lytic enzymeQi Cheng
Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, 1230 York Ave, New York, NY 10021, USA
Antimicrob Agents Chemother 49:111-7. 2005.... - Genomic sequence of C1, the first streptococcal phageDaniel Nelson
Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, New York, New York 10021, USA
J Bacteriol 185:3325-32. 2003..Likewise, comparative genomics revealed no close evolutionary matches, suggesting that the C(1) bacteriophage is a unique member of the Podoviridae... - Sortase A localizes to distinct foci on the Streptococcus pyogenes membraneAssaf Raz
Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
Proc Natl Acad Sci U S A 105:18549-54. 2008..The ability to study cytoplasmic and membrane antigens using deconvolution immunofluorescence microscopy will facilitate further study of cellular processes in S. pyogenes... - A genetic screen to identify bacteriophage lysinsRaymond Schuch
Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, New York, NY, USA
Methods Mol Biol 502:307-19. 2009.... - Phage lytic enzyme Cpl-1 as a novel antimicrobial for pneumococcal bacteremiaJutta M Loeffler
Laboratory of Bacterial Pathogenesis, The Rockefeller University, New York, New York 10021, USA
Infect Immun 71:6199-204. 2003..Bacteriophage enzymes are unusual but extremely effective antimicrobials and represent a new weapon against infections with resistant bacteria... - Novel method to control pathogenic bacteria on human mucous membranesVincent A Fischetti
The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA
Ann N Y Acad Sci 987:207-14. 2003..Thus, lysins may be added to our armamentarium to control antibiotic-resistant bacteria... - Mutagenesis of a bacteriophage lytic enzyme PlyGBS significantly increases its antibacterial activity against group B streptococciQi Cheng
The Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
Appl Microbiol Biotechnol 74:1284-91. 2007..A reduction in GBS colonization before delivery should significantly reduce neonatal GBS infection providing a safe alternative to IAP... - Characterization of a unique glycosylated anchor endopeptidase that cleaves the LPXTG sequence motif of cell surface proteins of Gram-positive bacteriaSung G Lee
Laboratory of Bacterial Pathogenesis Rockefeller University, New York, New York 10021, USA
J Biol Chem 277:46912-22. 2002.... - Role of the C-terminal lysine residues of streptococcal surface enolase in Glu- and Lys-plasminogen-binding activities of group A streptococciAnne Derbise
Laboratory of Bacterial Pathogenesis, Public Health Research Institute, International Center for Public Health, Newark, New Jersey 07103 3535, USA
Infect Immun 72:94-105. 2004.... - Purification and characterization of LPXTGase from Staphylococcus aureus: the amino acid composition mirrors that found in the peptidoglycanSung G Lee
Laboratory of Bacterial Pathogenesis, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
J Bacteriol 188:389-98. 2006..This suggests that enzymes responsible for wall assembly may also play a role in the construction of LPXTGase... - Presence of D-alanine in an endopeptidase from Streptococcus pyogenesSung G Lee
Laboratory of Bacterial Pathogenesis, Rockefeller University, New York, NY 10021, USA
J Biol Chem 278:46649-53. 2003.... - Novel algorithms reveal streptococcal transcriptomes and clues about undefined genesPatricia A Ryan
Department of Bacterial Pathogenesis and Immunology, Rockefeller University, New York, New York, USA
PLoS Comput Biol 3:e132. 2007..Neighbor clustering provides a more comprehensive view of the molecular responses of streptococci during pharyngeal cell adherence... - In vivo lysogenic conversion of Tox(-) Streptococcus pyogenes to Tox(+) with Lysogenic Streptococci or free phageThomas B Broudy
Laboratory of Bacterial Pathogenesis and Immunology, Rockefeller University, New York, New York 10021, USA
Infect Immun 71:3782-6. 2003..Ultimately, our findings demonstrate that the eukaryotic host serves as an essential component in the phage-mediated evolution of virulence within the microbial population... - A novel spore protein, ExsM, regulates formation of the exosporium in Bacillus cereus and Bacillus anthracis and affects spore size and shapeMonica M Fazzini
Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, New York, New York 10065, USA
J Bacteriol 192:4012-21. 2010..Insertional mutagenesis of exsM in Bacillus anthracis DeltaSterne resulted in a partial second exosporium and in smaller spores. In all, these findings suggest that ExsM plays a critical role in the formation of the exosporium... - Lysogeny of Streptococcus pneumoniae with MM1 phage: improved adherence and other phenotypic changesJutta M Loeffler
Laboratory of Bacterial Pathogenesis, The Rockefeller University, 1230 York Ave, New York, New York 10021, USA
Infect Immun 74:4486-95. 2006..By improving the adherence of pneumococci, this prophage may contribute to their fitness and possibly to their persistence in humans... - Synergistic killing of Streptococcus pneumoniae with the bacteriophage lytic enzyme Cpl-1 and penicillin or gentamicin depends on the level of penicillin resistanceSvetolik Djurkovic
Laboratory of Bacterial Pathogenesis, The Rockefeller University, 1230 York Ave, New York, NY 10021, USA
Antimicrob Agents Chemother 49:1225-8. 2005..Activities of Cpl-1 and gentamicin were increasingly synergistic with a decreasing penicillin MIC, while Cpl-1 and penicillin showed synergy against an extremely penicillin-resistant strain... - A structural basis for the allosteric regulation of non-hydrolysing UDP-GlcNAc 2-epimerasesLucas M Velloso
Laboratory of Structural Microbiology, The Rockfeller University, New York, NY 10021, USA
EMBO Rep 9:199-205. 2008..This constitutes the first example to our knowledge, of an enzymatic allosteric activation by direct interaction between the substrate and the allosteric activator... - Phage lytic enzyme Cpl-1 for antibacterial therapy in experimental pneumococcal meningitisDenis Grandgirard
Institute for Infectious Diseases, University of Bern, Switzerland
J Infect Dis 197:1519-22. 2008..Intraperitoneal administration of Cpl-1 (200 mg/kg) led to an antibacterial effect in CSF of 2 orders of magnitude for 3 h. Cpl-1 may hold promise as an alternative treatment option in pneumococcal meningitis... - Cysteine protease activity and histamine release from the human mast cell line HMC-1 stimulated by recombinant streptococcal pyrogenic exotoxin B/streptococcal cysteine proteaseYukino Watanabe
Department of Immunology and Infectious Diseases, Institute of Gerontology, Nippon Medical School, Nakahara-ku, Kawasaki 211-8533, Japan
Infect Immun 70:3944-7. 2002..This study may contribute to the understanding of the pathogenic role of SPE B/SCP in streptococcal infection and streptococcal toxic shock syndrome... - PlyC, a novel bacteriophage lysin for compartment-dependent proteomics of group A streptococciThomas Koller
Department of Medical Microbiology and Hospital Hygiene, Hospital of Rostock University, Schillingallee 70, Rostock, Germany
Proteomics 8:140-8. 2008..Furthermore, our experimental design allowed us to delineate the actual localization of the proteins despite contamination with intracellular proteins... - Reduced IFN-gamma responses associated with HLA-DR15 presentation of streptococcal cell wall proteins to dermal Th-1 cells in psoriasisBarbara S Baker
Department of Dermatology, Faculty of Medicine, Imperial College of Science, Technology and Medicine, London, United Kingdom
J Clin Immunol 23:407-14. 2003..It is proposed that HLA-DR allelic variation may contribute to disease phenotype via effects on the immune response to group A streptococci... - The 1.6 A crystal structure of the catalytic domain of PlyB, a bacteriophage lysin active against Bacillus anthracisCorrine J Porter
Protein Crystallography Unit, Department of Biochemistry and Molecular Biology and The ARC Centre of Excellence for Structural and Functional Microbial Genomics, Monash University, Clayton, Melbourne, VIC 3800, Australia
J Mol Biol 366:540-50. 2007..PlyB therefore represents a new class of anthrax lysin and a new defensive tool in the armament against anthrax-mediated bioterrorism... - Novel strategy to prevent otitis media caused by colonizing Streptococcus pneumoniaeJonathan A McCullers
Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, United States of America
PLoS Pathog 3:e28. 2007 - Biochemical analysis of a common human polymorphism associated with age-related macular degenerationJiamei Yu
Department of Physiology and Jules Stein Eye Institute, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
Biochemistry 46:8451-61. 2007..This study reveals the fundamental properties of a common polymorphism of CFH and lays the groundwork for elucidating the role of CFH in AMD pathogenesis... - Analysis of factors affecting surface expression and immunogenicity of recombinant proteins expressed by gram-positive commensal vectorsTove C Bolken
SIGA Technologies Inc, Corvallis, Oregon 97333, USA
Infect Immun 70:2487-91. 2002..The results obtained should facilitate the design of improved vaccine candidates using this antigen delivery technology... - Clinical and microbiological responses of volunteers to combined intranasal and oral inoculation with a Streptococcus gordonii carrier strain intended for future use as a group A streptococcus vaccineKaren L Kotloff
Department of Pediatrics, School of Medicine, University of Maryland, 685 West Baltimore St, HSF 480, Baltimore, MD 21201, USA
Infect Immun 73:2360-6. 2005..The results of these clinical trials provide encouragement that the use of S. gordonii as a live mucosal vaccine vector is a feasible strategy...
Research Grants
- Pathogen-specific drug targets for weaponized bacteriaVincent Fischetti; Fiscal Year: 2007..This proposal presents a concerted effort of three complementary groups at the Rockefeller University (Dr. Vincent Fischetti, Dr. Alexander Tomasz, and Dr. Erec Stebbins) to identify novel inhibitors of the Category A pathogens, B...
- Isolaton of new phage enzymes to kill B. anthracisVincent Fischetti; Fiscal Year: 2007..Because these enzymes may be an important line of defense against an attack with drug-resistant B. anthracis, having a number of enzymes at our disposal may allow for better decisions as to their use if necessary. ..
- PROPERTIES OF STREPTOCOCCAL M PROTEINVincent Fischetti; Fiscal Year: 2009....
- PROPERTIES OF STREPTOCOCCAL M PROTEINVincent A Fischetti; Fiscal Year: 2010....
- PROPERTIES OF STREPTOCOCCAL M-PROTEINVincent Fischetti; Fiscal Year: 1980..At present, two serologically unrelated M proteins have been purified and partially sequenced. Physiocochemical and sequence data indicate that the M molecules have a close structure relationship with mammalian tropomysin. ..
- Isolation of new phage enzymes to kill B. anthracisVincent A Fischetti; Fiscal Year: 2010..This latest information changes our thinking about the versatility of these pathogens, allowing us to devise innovative strategies to control them both in the environment and during infection. ..