STEVEN DANIEL DOUGLAS

Summary

Affiliation: The Children's Hospital of Philadelphia
Country: USA

Publications

  1. ncbi T-lymphocyte subsets in HIV-infected and high-risk HIV-uninfected adolescents: retention of naive T lymphocytes in HIV-infected adolescents. The Adolescent Medicine HIV/AIDS Research Network
    S D Douglas
    Department of Pediatrics, The Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, 19104 4318, USA
    Arch Pediatr Adolesc Med 154:375-80. 2000
  2. pmc TH1 and TH2 cytokine mRNA and protein levels in human immunodeficiency virus (HIV)-seropositive and HIV-seronegative youths
    Steven D Douglas
    Division of Allergy Immunology, Joseph Stokes, Jr Research Institute, The Children s Hospital of Philadelphia, and Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Clin Diagn Lab Immunol 10:399-404. 2003
  3. ncbi The relationships between substance abuse, psychosocial variables, and natural killer cell enumeration and function in HIV-infected and high-risk uninfected adolescents
    Steven D Douglas
    Division of Immunologic and Infectious Diseases, Department of Pediatrics, Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    AIDS Res Hum Retroviruses 19:399-408. 2003
  4. ncbi Hepatitis C, depressive symptoms, viral load, and therapy: interactions and reactions
    Steven D Douglas
    Division of Allergy and Immunology, Joseph Stokes Jr Research Institute at The Children s Hospital of Philadelphia, USA
    Brain Behav Immun 19:20-2. 2005
  5. ncbi NIMH Preclinical/Clinical Therapeutics Research meeting--preclinical drug discovery session
    Steven D Douglas
    Division of Allergy and Immunology, The Children s Hospital of Philadelphia, 34th Street and Civic Center Boulevard, Philadelphia, PA 19104 4399, USA
    J Neuroimmune Pharmacol 2:5-7. 2007
  6. doi Elevated substance P levels in HIV-infected women in comparison to HIV-negative women
    Steven D Douglas
    Division of Allergy and Immunology, Department of Pediatrics, Joseph Stokes Jr Research Institute at The Children s Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA
    AIDS Res Hum Retroviruses 24:375-8. 2008
  7. pmc Neurokinin-1 receptor mRNA expression differences in brains of HIV-infected individuals
    Steven D Douglas
    Division of Allergy and Immunology, Joseph Stokes Jr Research Institute at The Children s Hospital of Philadelphia, Department of Pediatrics, University of Pennsylvania School of Medicine, USA
    J Neurol Sci 272:174-7. 2008
  8. ncbi Neurokinin-1 receptor expression and function in human macrophages and brain: perspective on the role in HIV neuropathogenesis
    Steven D Douglas
    Division of Allergy and Immunology, Joseph Stokes Jr Research Institute at The Children s Hospital of Philadelphia, Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Ann N Y Acad Sci 1144:90-6. 2008
  9. pmc Neurokinin-1 receptor: functional significance in the immune system in reference to selected infections and inflammation
    Steven D Douglas
    Department of Pediatrics, Children s Hospital of Philadelphia Research Institute, University of Pennsylvania Medical School, Philadelphia, Pennsylvania, USA
    Ann N Y Acad Sci 1217:83-95. 2011
  10. ncbi Association of depression with viral load, CD8 T lymphocytes, and natural killer cells in women with HIV infection
    Dwight L Evans
    Department of Psychiatry, University of Pennsylvania School of Medicine, 305 Blockley Hall, Philadelphia, PA 19104 6021, USA
    Am J Psychiatry 159:1752-9. 2002

Research Grants

Detail Information

Publications82

  1. ncbi T-lymphocyte subsets in HIV-infected and high-risk HIV-uninfected adolescents: retention of naive T lymphocytes in HIV-infected adolescents. The Adolescent Medicine HIV/AIDS Research Network
    S D Douglas
    Department of Pediatrics, The Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, 19104 4318, USA
    Arch Pediatr Adolesc Med 154:375-80. 2000
    ..The capacity of the immune system of adolescents to generate and repopulate naive and memory cell populations under conditions of normal homeostasis and human immunodeficiency virus (HIV) infection is largely unknown...
  2. pmc TH1 and TH2 cytokine mRNA and protein levels in human immunodeficiency virus (HIV)-seropositive and HIV-seronegative youths
    Steven D Douglas
    Division of Allergy Immunology, Joseph Stokes, Jr Research Institute, The Children s Hospital of Philadelphia, and Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Clin Diagn Lab Immunol 10:399-404. 2003
    ..On the basis of our data, we conclude that, early in the course of HIV-associated disease in adolescents, there are no detectable shifts from TH1 to TH2 cytokine production...
  3. ncbi The relationships between substance abuse, psychosocial variables, and natural killer cell enumeration and function in HIV-infected and high-risk uninfected adolescents
    Steven D Douglas
    Division of Immunologic and Infectious Diseases, Department of Pediatrics, Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    AIDS Res Hum Retroviruses 19:399-408. 2003
    ..In addition, we determined the association of HIV disease stage, antiretroviral therapy (ART), CD4(+) T-cell count, and viral load with these outcomes in the subset of HIV-infected adolescents...
  4. ncbi Hepatitis C, depressive symptoms, viral load, and therapy: interactions and reactions
    Steven D Douglas
    Division of Allergy and Immunology, Joseph Stokes Jr Research Institute at The Children s Hospital of Philadelphia, USA
    Brain Behav Immun 19:20-2. 2005
  5. ncbi NIMH Preclinical/Clinical Therapeutics Research meeting--preclinical drug discovery session
    Steven D Douglas
    Division of Allergy and Immunology, The Children s Hospital of Philadelphia, 34th Street and Civic Center Boulevard, Philadelphia, PA 19104 4399, USA
    J Neuroimmune Pharmacol 2:5-7. 2007
  6. doi Elevated substance P levels in HIV-infected women in comparison to HIV-negative women
    Steven D Douglas
    Division of Allergy and Immunology, Department of Pediatrics, Joseph Stokes Jr Research Institute at The Children s Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA
    AIDS Res Hum Retroviruses 24:375-8. 2008
    ..We now demonstrate that substance P plasma levels are significantly higher in HIV-infected women in comparison to uninfected control women...
  7. pmc Neurokinin-1 receptor mRNA expression differences in brains of HIV-infected individuals
    Steven D Douglas
    Division of Allergy and Immunology, Joseph Stokes Jr Research Institute at The Children s Hospital of Philadelphia, Department of Pediatrics, University of Pennsylvania School of Medicine, USA
    J Neurol Sci 272:174-7. 2008
    ..These preliminary data support the concept that the full-length form of NK1R may have important significance in cognitive functions and deficiency of this isoform may be relevant in neurologic and psychiatric manifestations of neuroAIDS...
  8. ncbi Neurokinin-1 receptor expression and function in human macrophages and brain: perspective on the role in HIV neuropathogenesis
    Steven D Douglas
    Division of Allergy and Immunology, Joseph Stokes Jr Research Institute at The Children s Hospital of Philadelphia, Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Ann N Y Acad Sci 1144:90-6. 2008
    ..g., THP-1 cells); and differences in regional tissue distribution (e.g., differences in different brain regions). NK1R-SP receptor pathways are important cell regulatory pathways...
  9. pmc Neurokinin-1 receptor: functional significance in the immune system in reference to selected infections and inflammation
    Steven D Douglas
    Department of Pediatrics, Children s Hospital of Philadelphia Research Institute, University of Pennsylvania Medical School, Philadelphia, Pennsylvania, USA
    Ann N Y Acad Sci 1217:83-95. 2011
    ..The proinflammatory signals modulated by SP are important in bacterial, viral, fungal, and parasitic diseases, as well as in immune system function. The SP-NK1R system is a major class 1, rhodopsin-like GPCR ligand-receptor interaction...
  10. ncbi Association of depression with viral load, CD8 T lymphocytes, and natural killer cells in women with HIV infection
    Dwight L Evans
    Department of Psychiatry, University of Pennsylvania School of Medicine, 305 Blockley Hall, Philadelphia, PA 19104 6021, USA
    Am J Psychiatry 159:1752-9. 2002
    ..This study sought to determine if depression was associated with alterations in cellular immunity variables-specifically, natural killer (NK) cells and CD8 T lymphocytes-in women with HIV infection...
  11. pmc Analyses of HIV-1 drug-resistance profiles among infected adolescents experiencing delayed antiretroviral treatment switch after initial nonsuppressive highly active antiretroviral therapy
    Allison Agwu
    Department of Pediatrics, Division of Infectious Diseases, Johns Hopkins Medical Institution, Baltimore, Maryland, USA
    AIDS Patient Care STDS 22:545-52. 2008
    ..This finding may have implications for initial treatment regimens and transmission risk in HIV-infected youth, a group with rising infection rates...
  12. ncbi Depressive and anxiety disorders in women with HIV infection
    Mary F Morrison
    Center for Clinical Epidemiolgy and Biostatistics and Department of Psychiatry, University of Pennsylvania School of Medicine, Philadelphia, 19104 6021, USA
    Am J Psychiatry 159:789-96. 2002
    ....
  13. pmc Neurokinin-1 receptor antagonist (aprepitant) suppresses HIV-1 infection of microglia/macrophages
    Xu Wang
    Department of Pediatrics, Joseph Stokes, Jr Research Institute at The Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Neuroimmune Pharmacol 3:257-64. 2008
    ..These observations provide additional evidence to support the potential use of NK-1R antagonists as therapeutic and immunomodulatory agents for the treatment of HIV-1 infection...
  14. ncbi A non-peptide substance P antagonist down-regulates SP mRNA expression in human mononuclear phagocytes
    Jian Ping Lai
    Division of Immunologic and Infectious Diseases, Joseph Stokes Jr Research Institute at The Children s Hospital of Philadelphia, Department of Pediatrics, University of Pennsylvania Medical School, Philadelphia, PA 19104, USA
    J Neuroimmunol 128:101-8. 2002
    ..Since SP plays an important role in inflammation and viral infections, these studies may have potential applications for therapeutic intervention of inflammation and viral infection of immune cells...
  15. ncbi Phase I/II trial of intravenous recombinant interleukin-2 in HIV-infected children
    Stuart E Starr
    Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, USA
    AIDS 17:2181-9. 2003
    ..To define the tolerated dose of recombinant interleukin-2 (rIL-2) in HIV-infected children (part A), and to determine the safety and immunologic effects of the tolerated rIL-2 dose in a cohort of HIV-infected children (part B)...
  16. ncbi CD8+CD38+ T cells but not HIV type 1 RNA viral load predict CD4+ T cell loss in a predominantly minority female HIV+ adolescent population
    Craig M Wilson
    Department of Pediatrics, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    AIDS Res Hum Retroviruses 20:263-9. 2004
    ..This study extends the observed association of higher CD8(+) CD38(+) T cells numbers being predictive of HIV-1 disease progression into predominantly female, minority youth...
  17. ncbi Morphine inhibits CD8+ T cell-mediated, noncytolytic, anti-HIV activity in latently infected immune cells
    Xu Wang
    The Children s Hospital of Philadelphia, 34th Street and Civic Center Boulevard, Philadelphia, PA 19104, USA
    J Leukoc Biol 78:772-6. 2005
    ..These data provide additional evidence to support the notion that opioids play a role in impairing the anti-HIV function of the immune system...
  18. pmc Selective serotonin reuptake inhibitor and substance P antagonist enhancement of natural killer cell innate immunity in human immunodeficiency virus/acquired immunodeficiency syndrome
    Dwight L Evans
    Department of Psychiatry, Joseph J Stokes Research Institute of The Children s Hospital of Philadelphia, Philadelphia, Pennsylvania, USA
    Biol Psychiatry 63:899-905. 2008
    ..This study examines the potential role of three underlying regulatory systems that have been under investigation in central nervous system research as well as immune and viral research: serotonin, neurokinin, and glucocorticoid systems...
  19. pmc Methylnaltrexone antagonizes opioid-mediated enhancement of HIV infection of human blood mononuclear phagocytes
    Wen Zhe Ho
    Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
    J Pharmacol Exp Ther 307:1158-62. 2003
    ..The ability of MNTX to block opioid-induced CCR5 expression and HIV replication at clinically relevant doses may have additional benefit for opioid abusers with HIV infection, or patients with AIDS pain receiving opioids...
  20. ncbi Longitudinal analysis of lymphocyte ratios and HIV-1 intracellular DNA levels in children
    Akihiko Saitoh
    Department of Pediatrics, Division of Infectious Diseases, University of California, San Diego, La Jolla, California 92093 0672, USA
    J Infect Dis 189:1216-20. 2004
    ..These data suggest that the increased proportion of naive lymphocytes found in children are less susceptible to HIV-1 infection than are the memory lymphocytes that dominate immune reconstitution in adults...
  21. doi Neurokinin 1 receptor isoforms and the control of innate immunity
    Florin Tuluc
    Joseph Stokes Jr Research Institute, The Children s Hospital of Philadelphia, USA
    Trends Immunol 30:271-6. 2009
    ..Targeting the truncated NK1R with pharmacologic agents might result in novel therapeutic approaches in diseases which affect the immune system, including HIV disease...
  22. ncbi Neurokinin-1 receptor antagonist (aprepitant) inhibits drug-resistant HIV-1 infection of macrophages in vitro
    Xu Wang
    Division of Allergy and Immunology, Children s Hospital of Philadelphia, Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
    J Neuroimmune Pharmacol 2:42-8. 2007
    ..Aprepitant is in clinical use for the prevention of nausea and vomiting associated with cancer chemotherapy or following surgical procedures...
  23. pmc Novel method for determination of substance P levels in unextracted human plasma by using acidification
    Donald E Campbell
    Joseph Stokes Jr Research Institute at The Children s Hospital of Philadelphia, Department of Pediatrics, University of Pennsylvania School of Medicine, USA
    Clin Vaccine Immunol 16:594-6. 2009
    ..SP is detectable in plasma in a free and bound state. Simple modification of a commercially available SP enzyme-linked immunosorbent assay allows the dissociation and capture of plasma SP without solid-phase extraction...
  24. ncbi Increased proliferation within T lymphocyte subsets of HIV-infected adolescents
    Stuart E Starr
    Department of Pediatrics, Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 4318, USA
    AIDS Res Hum Retroviruses 18:1301-10. 2002
    ..The Ki-67 assay provided new and useful information on in vivo lymphocyte proliferation in HIV-infected adolescents...
  25. pmc Virologic and immunologic correlates with the magnitude of antibody responses to the hepatitis A vaccine in HIV-infected children on highly active antiretroviral treatment
    Adriana Weinberg
    Department of Pediatrics, University of Colorado Denver, Aurora, CO 80045, USA
    J Acquir Immune Defic Syndr 52:17-24. 2009
    ..We sought to identify the immunologic and virologic factors associated with a robust response to hepatitis A virus (HAV) vaccine in children on highly active antiretroviral treatment...
  26. pmc Morphine enhances HIV infection of human blood mononuclear phagocytes through modulation of beta-chemokines and CCR5 receptor
    Chang Jiang Guo
    Division of Immunologic and Infectious Diseases, Joseph Stokes Jr Research Institute of The Children s Hospital of Philadelphia, PA 19104, USA
    J Investig Med 50:435-42. 2002
    ..Injection drug use remains a significant risk for acquiring HIV infection. The mechanisms by which morphine enhances HIV infection of human immune cells are largely unknown...
  27. ncbi Immune reconstitution and predictors of virologic failure in adolescents infected through risk behaviors and initiating HAART: week 60 results from the PACTG 381 cohort
    Bret J Rudy
    Children s Hospital of Philadelphia and University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    AIDS Res Hum Retroviruses 22:213-21. 2006
    ..Several T cell markers were predictive of subsequent virologic failure in subjects achieving short-term success. Further study is warranted to determine whether these predictors provide any benefit to clinical management...
  28. ncbi Association of depression, CD8+ T lymphocytes, and natural killer cell activity: implications for morbidity and mortality in Human immunodeficiency virus disease
    Dean G Cruess
    Department of Psychology, University of Pennsylvania, 3815 Walnut Street, Philadelphia, PA 19104, USA
    Curr Psychiatry Rep 5:445-50. 2003
    ....
  29. pmc A sensitive and rapid liquid chromatography-tandem mass spectrometry method for the quantification of the novel neurokinin-1 receptor antagonist aprepitant in rhesus macaque plasma, and cerebral spinal fluid, and human plasma with application in translati
    Di Wu
    Laboratory for Applied PK PD, Division of Clinical Pharmacology and Therapeutics, The Children s Hospital of Philadelphia, Philadelphia, PA 19104, USA
    J Pharm Biomed Anal 49:739-45. 2009
    ....
  30. pmc Measurement of plasma-derived substance P: biological, methodological, and statistical considerations
    Donald E Campbell
    Division of Allergy and Immunology, Joseph Stokes Jr Research Institute, The Children s Hospital of Philadelphia, 34th and Civic Center Blvd, Philadelphia, PA 19104, USA
    Clin Vaccine Immunol 13:1197-203. 2006
    ..These results further emphasize the need for careful consideration in the selection of methods for SP quantitation, as well as caution in the interpretation and comparison of data reported in the literature...
  31. ncbi Efavirenz liquid formulation in human immunodeficiency virus-infected children
    Stuart E Starr
    Division of Infectious and Immunologic Diseases, Abramson Research Center, The Children s Hospital of Philadelphia, PA 19104, USA
    Pediatr Infect Dis J 21:659-63. 2002
    ....
  32. pmc Peripheral blood lymphocyte subsets in adolescents: a longitudinal analysis from the REACH project
    Bret J Rudy
    Children s Hospital of Philadelphia, Department of Pediatrics, The University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, 19104, USA
    Clin Diagn Lab Immunol 9:959-65. 2002
    ..Race and/or ethnicity was associated with differences in total CD8(+) cell counts and CD8(+) memory cell counts, although both of these associations involved an interaction with gender...
  33. ncbi Alcohol potentiates hepatitis C virus replicon expression
    Ting Zhang
    Division of Allergy and Immunology, Joseph Stokes Jr Research Institute at The Children s Hospital of Philadelphia, Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Hepatology 38:57-65. 2003
    ..Thus, alcohol may play an important role in vivo as a cofactor in HCV disease progression and compromise IFN-alpha-based therapy against HCV infection...
  34. ncbi Association of resolution of major depression with increased natural killer cell activity among HIV-seropositive women
    Dean G Cruess
    Department of Psychology, University of Pennsylvania, Philadelphia, USA
    Am J Psychiatry 162:2125-30. 2005
    ..This study examined whether improvements in the diagnostic status of major depression are related to increases in NK cell activity among HIV-seropositive women...
  35. ncbi Morphine upregulates functional expression of neurokinin-1 receptor in neurons
    Qi Wan
    Division of Allergy and Immunology, Joseph Stokes Jr Research Institute at The Children s Hospital of Philadelphia, Department of Pediatrics, Philadelphia, Pennsylvania
    J Neurosci Res 84:1588-96. 2006
    ..These in vitro data provide a plausible cellular mechanism for opioid-mediated neurological disorders...
  36. pmc Methadone enhances human immunodeficiency virus infection of human immune cells
    Yuan Li
    Division of Immunologic and Infectious Diseases, Joseph Stokes Jr Research Institute of The Children s Hospital of Philadelphia, Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Infect Dis 185:118-22. 2002
    ..Although the in vivo relevance of these findings remains to be determined, the data underscore the necessity of further studies to define the role of opioids, including methadone, in the immunopathogenesis of HIV infection and AIDS...
  37. ncbi CD8+ T cell depletion amplifies hepatitis C virus replication in peripheral blood mononuclear cells
    Yuan Li
    Division of Allergy and Immunology, Department of Pediatrics, Joseph Stokes Jr Research Institute, The Children s Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA
    J Infect Dis 192:1093-101. 2005
    ..These findings may have important implications for the immunopathogenesis of HCV in both immune and hepatic cells and are relevant to the development of host innate immunity-based anti-HCV interventions...
  38. ncbi Naltrexone inhibits alcohol-mediated enhancement of HIV infection of T lymphocytes
    Xu Wang
    Division of Allergy and Immunology, Joseph Stokes Jr Research Institute at The Children s Hospital of Philadelphia, 34th Street and Civic Center Boulevard, Philadelphia, PA 19104, USA
    J Leukoc Biol 79:1166-72. 2006
    ..These findings suggest a biological mechanism for the potential therapeutic benefit of naltrexone in treating HIV-infected alcoholics...
  39. ncbi Natural killer cells inhibit hepatitis C virus expression
    Yuan Li
    Division of Allergy and Immunology, Joseph Stokes, Jr Research Institute at The Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, 34th Street and Civic Center Blvd, Philadelphia, PA 19104, USA
    J Leukoc Biol 76:1171-9. 2004
    ..Thus, NK cells hold the potential to play a vital role in controlling HCV replication in hepatic cells using an IFN-gamma-dependent mechanism...
  40. pmc Substance P (SP) enhances CCL5-induced chemotaxis and intracellular signaling in human monocytes, which express the truncated neurokinin-1 receptor (NK1R)
    Irene Chernova
    Department of Pediatrics, University of Pennsylvania School of Medicine and the Joseph Stokes Jr Research Institute, Children s Hospital of Philadelphia, 3615 Civic Center Blvd, Philadelphia, PA 19104, USA
    J Leukoc Biol 85:154-64. 2009
    ..Thus, NK1R-T is functional in human monocytes and activates select signaling pathways, and the NK1R-T-mediated enhancement of CCL5 responses does not require the NK1R terminal cytoplasmic domain...
  41. pmc CCR5 expression and beta-chemokine production during placental neonatal monocyte differentiation
    Dylan Zylla
    Division of Immunologic and Infectious Diseases, The Children s Hospital of Philadelphia, PA 19104, USA
    Pediatr Res 53:853-8. 2003
    ..CCR5 receptor plays a critical role in HIV infection of neonatal blood monocyte/macrophages...
  42. pmc Morphine enhances HIV infection of neonatal macrophages
    Yuan Li
    Division of Immunologic and Infectious Diseases, The Children s Hospital of Philadelphia, 34th Street and Civic Center Boulevard, Philadelphia, PA 19104, U S A
    Pediatr Res 54:282-8. 2003
    ..Thus, morphine, most likely through alteration of beta-chemokines and CCR5 receptor expression, enhances the susceptibility of neonatal MDM to HIV infection, and may have a cofactor role in perinatal HIV transmission and infection...
  43. ncbi Substance P and neurokinin-1 receptor modulation of HIV
    Wen Zhe Ho
    Division of Allergy and Immunology, Joseph Stokes Jr Research Institute at Children s Hospital of Philadelphia, Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Neuroimmunol 157:48-55. 2004
    ..Modulation of SP activity and SP receptor may offer a novel approach to the treatment of psychiatric disorders and to the design of new anti-HIV therapy...
  44. pmc An in vitro model of morphine withdrawal manifests the enhancing effect on human immunodeficiency virus infection of human T lymphocytes through the induction of substance P
    Xu Wang
    Department of Pediatrics, Division of Allergy and Immunology, Joseph Stokes Jr Research Institute at The Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Am J Pathol 169:1663-70. 2006
    ..These findings provide a cellular mechanism that supports the notion that opioids have a co-factor role in promoting HIV infection of the immune cells...
  45. pmc Quantification of substance p mRNA in human immune cells by real-time reverse transcriptase PCR assay
    Jian Ping Lai
    Division of Immunologic and Infectious Diseases, Children s Hospital of Philadelphia, 34th and Civic Center Blvd, Philadelphia, PA 19104, USA
    Clin Diagn Lab Immunol 9:138-43. 2002
    ..Since SP is a major modulator of neuroimmunoregulation, this assay has the potential for widespread application for basic and clinical investigations...
  46. ncbi HIV enhances substance P expression in human immune cells
    Wen Zhe Ho
    The Children s Hospital of Philadelphia, Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    FASEB J 16:616-8. 2002
    ..These data support the hypothesis that interaction of HIV and SP may have significant in vivo relevance to the immunopathogenesis of HIV infection and AIDS...
  47. pmc Innate immune markers in mothers and fathers of children newly diagnosed with cancer
    Meredith J Lutz Stehl
    Division of Oncology, The Children s Hospital of Philadelphia, Philadelphia, PA 19104 4399, USA
    Neuroimmunomodulation 15:102-7. 2008
    ..Despite associations between stress and immune function in animal and human models, the immune function in caregivers of children at the time of a child-related potentially traumatic event, like cancer, is not known...
  48. ncbi Natural killer cell inhibits human immunodeficiency virus replication in chronically infected immune cells
    Ting Zhang
    Division of Allergy and Immunology, Joseph Stokes Jr Research Institute of The Children s Hospital of Philadelphia, Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Antiviral Res 73:132-9. 2007
    ..The P38 MAPK inhibitor (SB 203580) blocked NK SN-mediated HIV inhibition. These data provide compelling evidence that NK cells have a critical role in controlling HIV activation in the reservoirs...
  49. pmc Morphine withdrawal enhances hepatitis C virus replicon expression
    Chuan qing Wang
    Department of Pediatrics, Division of Allergy and Immunology, The Children s Hospital of Philadelphia, and the Department of Psychiatry, University of Pennsylvania School of Medicine, 34th St and Civic Center Blvd, Philadelphia, PA 19104, USA
    Am J Pathol 167:1333-40. 2005
    ..These in vitro observations support the concept that opioid abuse favors HCV persistence in hepatic cells by suppressing IFN-alpha-mediated intracellular innate immunity and contributes to the development of chronic HCV infection...
  50. ncbi Depression and HIV infection: impact on immune function and disease progression
    Dean G Cruess
    Department of Psychology, University of Pennsylvania, 3815 Walnut Street, Philadelphia, PA 19104, USA
    CNS Spectr 8:52-8. 2003
    ..This paper will highlight studies examining the prevalence of depression during HIV infection and review some of the evidence examining the impact of depressive symptoms on immune function and HIV disease progression...
  51. pmc Human neuronal cells (NT2-N) express functional substance P and neurokinin-1 receptor coupled to MIP-1 beta expression
    Yuan Li
    Division of Immunologic and Infectious Diseases, Joseph Stokes, Jr Research Institute at The Children s Hospital of Philadelphia, Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA
    J Neurosci Res 71:559-66. 2003
    ..The NT2-N cell line is well suited for in vitro investigations of the SP-NK-1R pathway in immune responses and inflammation in the CNS...
  52. pmc Alcohol potentiates HIV-1 infection of human blood mononuclear phagocytes
    Xu Wang
    Division of Immunologic and Infectious Disease, Joseph Stokes Jr Research Institute at The Children s Hospital of Philadelphia, Department of Pediatrics, Pennsylvania 19104, USA
    Alcohol Clin Exp Res 26:1880-6. 2002
    ..The mechanisms by which alcohol affects the function of human immune cells that are the targets for HIV are unknown...
  53. pmc Differences in the length of the carboxyl terminus mediate functional properties of neurokinin-1 receptor
    Jian Ping Lai
    Division of Allergy and Immunology, Joseph Stokes Jr Research Institute at The Children s Hospital of Philadelphia, Department of Pediatrics, University of Pennsylvania Medical School, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 105:12605-10. 2008
    ..These differences may provide important information toward the design of new NK1R receptor antagonists...
  54. pmc Morphine enhances hepatitis C virus (HCV) replicon expression
    Yuan Li
    Division of Immunologic and Infectious Diseases, Department of Pediatrics, Stokes Research Institute, Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, 34th Street and Civic Center Boulevard, Philadelphia, PA 19104, USA
    Am J Pathol 163:1167-75. 2003
    ..Our in vitro data indicate that morphine may play an important role as a positive regulator of HCV replication in human hepatic cells and may compromise IFN-alpha therapy...
  55. pmc Interleukin-1beta induces macrophage inflammatory protein-1beta expression in human hepatocytes
    Ting Zhang
    Division of Allergy and Immunology, Joseph Strokes Jr Research Institute at The Children s Hospital of Philadelphia, Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Cell Immunol 226:45-53. 2003
    ..These observations suggest that IL-1beta-mediated up-regulation of MIP-1beta production in the hepatic cells may contribute a critical mechanism for continuous recruitment of inflammatory cell to liver and maintenance of inflammation...
  56. ncbi A non-peptide substance P antagonist (CP-96,345) inhibits morphine-induced NF-kappa B promoter activation in human NT2-N neurons
    Xu Wang
    Division of Immunologic and Infectious Diseases, Joseph Stokes Jr Research Institute of The Children s Hospital of Philadelphia, Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA
    J Neurosci Res 75:544-53. 2004
    ..Activation of NK-1R could then impact NF-kappaB expression and therefore may be an important participant in the effect of morphine on immune responses in the central nervous system...
  57. ncbi Prevalence, diagnosis, and pharmacological treatment of mood disorders in HIV disease
    Dean G Cruess
    Department of Psychology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Biol Psychiatry 54:307-16. 2003
    ....
  58. ncbi Mimic-based RT-PCR quantitation of substance P mRNA in human mononuclear phagocytes and lymphocytes
    Jian Ping Lai
    Division of Immunologic and Infectious Diseases, Joseph Stokes Jr Research Institute at The Children s Hospital of Philadelphia, PA, USA
    Methods Mol Biol 193:129-47. 2002
  59. pmc Alcohol suppresses IL-2-induced CC chemokine production by natural killer cells
    Ting Zhang
    Department of Pediatrics, Division of Allergy and Immunology, Joseph Stokes Jr Research Institute at The Children s Hospital of Philadelphia, PA 19104, USA
    Alcohol Clin Exp Res 29:1559-67. 2005
    ..We investigated whether alcohol impairs NK cell function, particularly production of CC chemokines induced by interleukin (IL)-2, the natural ligands for CCR5 receptor...
  60. pmc Quantification of CCR5 mRNA in human lymphocytes and macrophages by real-time reverse transcriptase PCR assay
    Jian Ping Lai
    Divisions of Immunologic and Infectious Diseases, Joseph Stokes, Jr, Research Institute, The Children s Hospital of Philadelphia, Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Clin Diagn Lab Immunol 10:1123-8. 2003
    ..MDM expressed higher levels of CCR5 mRNA than did PBL. Thus, this assay has the potential and a wide application for the investigation of the role of CCR5 in inflammatory diseases and viral infections, including HIV disease...
  61. pmc Incidence of noninfectious conditions in perinatally HIV-infected children and adolescents in the HAART era
    Sharon A Nachman
    Department of Pediatrics, Stony Brook University Medical Center, Stony Brook, NY 11794 8111, USA
    Arch Pediatr Adolesc Med 163:164-71. 2009
    ....
  62. pmc Interleukin-1beta upregulates functional expression of neurokinin-1 receptor (NK-1R) via NF-kappaB in astrocytes
    Chang Jiang Guo
    Division of Allergy and Immunology, Department of Pediatrics, Joseph Stokes Jr Research Institute of Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Glia 48:259-66. 2004
    ..These findings provide additional evidence that there is a biological interaction between IL-1beta and the neuropeptide SP in the CNS, which may have important implications in the inflammatory diseases in the CNS...
  63. pmc A phase I-II study of live attenuated varicella-zoster virus vaccine to boost immunity in human immunodeficiency virus-infected children with previous varicella
    Anne A Gershon
    Department of Pediatrics, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    Pediatr Infect Dis J 28:653-5. 2009
    ..There were no serious adverse events. Two years after vaccination 82% of subjects remained VZV-antibody positive and 60% had VZV-specific cell-mediated immunity. No child developed herpes zoster...
  64. ncbi Hepatitis C virus inhibits intracellular interferon alpha expression in human hepatic cell lines
    Ting Zhang
    Division of Allergy and Immunology, Joseph Stokes Jr Research Institute at The Children s Hospital of Philadelphia, Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Hepatology 42:819-27. 2005
    ..In conclusion, we provide direct evidence that HCV undermines the intracellular innate immunity of the target cells, which may account for HCV persistence in hepatic cells...
  65. ncbi CD4+ and CD8+ T cell receptor repertoire perturbations with normal levels of T cell receptor excision circles in HIV-infected, therapy-naive adolescents
    Savita Pahwa
    Immunology and Inflammation Center of Excellence, North Shore Long Island Jewish Health System and Research Institute, Manhasset, New York 11030, USA
    AIDS Res Hum Retroviruses 19:487-95. 2003
    ....
  66. ncbi Host genetic profiles predict virological and immunological control of HIV-1 infection in adolescents
    Jianming Tang
    Department of Medicine, University of Alabama at Birmingham, AL 39294 0022, USA
    AIDS 16:2275-84. 2002
    ..To evaluate the correlation between host genetic profiles and virological and immunological outcomes among HIV-1-seropositive participants from the Reaching for Excellence in Adolescent Care and Health (REACH) cohort...
  67. pmc Assessment of thymic activity in human immunodeficiency virus-negative and -positive adolescents by real-time PCR quantitation of T-cell receptor rearrangement excision circles
    Thao Pham
    David Geffen School of Medicine, University of California, Los Angeles, California, USA
    Clin Diagn Lab Immunol 10:323-8. 2003
    ..These data demonstrate the utility of TREC assessment in adolescents and that HIV infection does not uniformly result in accelerated thymic involution in childhood...
  68. pmc Long-term effects of highly active antiretroviral therapy on CD4+ cell evolution among children and adolescents infected with HIV: 5 years and counting
    Kunjal Patel
    Department of Epidemiology, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    Clin Infect Dis 46:1751-60. 2008
    ....
  69. pmc Association of site-specific and participant-specific factors with retention of children in a long-term pediatric HIV cohort study
    Paige L Williams
    Center for Biostatistics in AIDS Research, Harvard School of Public Health, Boston, MA 02115 6017, USA
    Am J Epidemiol 167:1375-86. 2008
    ..Although participant characteristics are difficult to modify, the authors identified several potentially modifiable site practices that could be targeted to improve retention...
  70. ncbi Lymphocyte subsets in healthy children from birth through 18 years of age: the Pediatric AIDS Clinical Trials Group P1009 study
    William T Shearer
    Baylor College of Medicine, Houston, Tex, USA
    J Allergy Clin Immunol 112:973-80. 2003
    ....
  71. ncbi HIV-1 vaccine induced immune responses in newborns of HIV-1 infected mothers
    Elizabeth J McFarland
    Department of Pediatric Infectious Diseases, University of Colorado Health Sciences Center, Denver Colorado, USA
    AIDS 20:1481-9. 2006
    ..This study evaluated the safety and immunogenicity of ALVAC HIV-1 vaccine with and without a subunit envelope boost in infants born to HIV-1-infected women...
  72. ncbi Plasma cytokines and oxidative damage in HIV-positive and HIV-negative adolescents and young adults: a protective role for IL-10?
    Charles B Stephensen
    USDA ARS Western Human Nutrition Research Center at the University of California, Davis and Department of Nutrition, University of California, Davis, CA 95616, USA
    Free Radic Res 39:859-64. 2005
    ..This finding is consistent with a protective role for IL-10 in diminishing oxidative damage during immune activation...
  73. ncbi Immune activation and oxidative damage in HIV-positive and HIV-negative adolescents
    Charles B Stephensen
    United States Department of Agriculture Western Human Nutrition Research Center and Department of Nutrition, University of California at Davis, Davis, CA 95616, USA
    J Acquir Immune Defic Syndr 38:180-90. 2005
    ..Plasma ceruloplasmin was associated with decreased oxidative damage in HIV-positive subjects, although this association was not seen in those taking ART...
  74. ncbi Virologic and immunologic outcomes after 24 weeks in HIV type 1-infected adolescents receiving highly active antiretroviral therapy
    Patricia M Flynn
    Department of Infectious Diseases, St Jude Children s Research Hospital, University of Tennessee Health Science Center, Memphis, Tennessee, USA
    J Infect Dis 190:271-9. 2004
    ..Adolescents represent the fastest growing demographic group of new human immunodeficiency virus (HIV) infections in the United States. At present, there is little information available about their response to therapy...
  75. ncbi Glutathione, glutathione peroxidase, and selenium status in HIV-positive and HIV-negative adolescents and young adults
    Charles B Stephensen
    US Department of Agriculture Agricultural Research Service, Western Human Nutrition Research Center, University of California, Davis, CA 95616, USA
    Am J Clin Nutr 85:173-81. 2007
    ..Antioxidant nutrient deficiencies may hasten the progression of HIV disease by impairing antioxidant defenses...
  76. ncbi Cytokine and chemokine gene polymorphisms among ethnically diverse North Americans with HIV-1 infection
    Chengbin Wang
    Department of Epidemiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Acquir Immune Defic Syndr 35:446-54. 2004
    ....
  77. ncbi Long-term observation of adolescents initiating HAART therapy: three-year follow-up
    Patricia M Flynn
    Department of Infectious Diseases, St Jude Children s Research Hospital, University of Tennessee Health Science Center, Memphis, Tennessee 38105, USA
    AIDS Res Hum Retroviruses 23:1208-14. 2007
    ..Interventions, especially those focused on adherence, are necessary to improve HAART outcomes in adolescents...
  78. ncbi CCR2 and CCR5 genotypes in HIV type 1-infected adolescents: limited contributions to variability in plasma HIV type 1 RNA concentration in the absence of antiretroviral therapy
    Jianming Tang
    Division of Geographic Medicine, Department of Medicine, Schools of Public Health and Medicine, 1665 University Blvd, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    AIDS Res Hum Retroviruses 18:403-12. 2002
    ..001). Thus, among the major CCR2-CCR5 haplotypes/genotypes in chronically infected and predominantly African-American adolescents, only the E/E genotype appeared to influence early host-virus equilibration...
  79. ncbi Relationship of plasma HIV-1 RNA dynamics to baseline factors and virological responses to highly active antiretroviral therapy in adolescents (aged 12-22 years) infected through high-risk behavior
    Hulin Wu
    Department of Biostatistics and Computational Biology, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642, USA
    J Infect Dis 189:593-601. 2004
    ....
  80. pmc Perceived stress is associated with impaired T-cell response to HPV16 in women with cervical dysplasia
    Carolyn Y Fang
    Division of Population Science, Fox Chase Cancer Center, Cheltenham, PA 19012, USA
    Ann Behav Med 35:87-96. 2008
    ..Research suggests that stress is associated with cervical disease progression, but few studies have examined the biological mechanisms that may be driving this association...
  81. pmc Association of hypercholesterolemia incidence with antiretroviral treatment, including protease inhibitors, among perinatally HIV-infected children
    Katherine Tassiopoulos
    Center for Biostatistics in AIDS Research, Harvard School of Public Health, Boston, MA 02115, USA
    J Acquir Immune Defic Syndr 47:607-14. 2008
    ..Antiretroviral therapy has been associated with hypercholesterolemia in HIV-infected children. Few longitudinal studies have been conducted to examine this association, however...
  82. ncbi Neuropsychopharmacologic treatment of depression and other neuropsychiatric disorders in HIV-infected individuals
    Martin J Repetto
    McKnight Brain Institute, Department of Psychiatry, University of Florida College of Medicine, Gainesville, FL 32610 0256, USA
    CNS Spectr 8:59-63. 2003
    ..Taken together, there is compelling evidence that psychopharmacologic intervention can improve the quality of life of mentally ill HIV-infected individuals...

Research Grants27

  1. Tachykinins Mononuclear Phagocytes and HIV-1 Infection
    Steven Douglas; Fiscal Year: 2005
    ..These proposed studies are a unique series of experiments directed toward elucidating the mechanistic role of SP and the SP receptor in the crucial links between psychosocial factors, depression and HIV/AIDS disease progression. ..
  2. Interaction of Human Immune Cells Alcohol & HIV
    Steven Douglas; Fiscal Year: 2005
    ..These studies may provide new mechanistic insights related to the role of alcohol in the pathogenesis of HIV disease and AIDS and toward design of new therapeutic approaches. ..
  3. PHILADELPHIA PEDIATRIC AIDS CLINICAL TRIALS UNIT
    Steven Douglas; Fiscal Year: 2006
    ..abstract_text> ..
  4. Tachykinins Mononuclear Phagocytes and HIV-1 Infection
    Steven Douglas; Fiscal Year: 2006
    ..These proposed studies are a unique series of experiments directed toward elucidating the mechanistic role of SP and the SP receptor in the crucial links between psychosocial factors, depression and HIV/AIDS disease progression. ..
  5. Philadelphia IMPAACT Clinical Trials Unit
    Steven Douglas; Fiscal Year: 2007
    ..administrative component: ..
  6. Tachykinins Mononuclear Phagocytes and HIV-1 Infection
    Steven Douglas; Fiscal Year: 2007
    ..These proposed studies are a unique series of experiments directed toward elucidating the mechanistic role of SP and the SP receptor in the crucial links between psychosocial factors, depression and HIV/AIDS disease progression. ..
  7. Tachykinins Mononuclear Phagocytes and HIV-1 Infection
    Steven Douglas; Fiscal Year: 2009
    ..Relevance to Public Health: These studies will further lead to understanding the pathogenesis of neurocognitive changes in HIV disease and lead to unique and novel therapeutic intervention. ..
  8. Interaction of Human Immune Cells Alcohol & HIV
    Steven Douglas; Fiscal Year: 2004
    ..These studies may provide new mechanistic insights related to the role of alcohol in the pathogenesis of HIV disease and AIDS and toward design of new therapeutic approaches. ..
  9. Tachykinins Mononuclear Phagocytes and HIV-1 Infection
    Steven Douglas; Fiscal Year: 2004
    ..These proposed studies are a unique series of experiments directed toward elucidating the mechanistic role of SP and the SP receptor in the crucial links between psychosocial factors, depression and HIV/AIDS disease progression. ..
  10. Interaction of Human Immune Cells Alcohol & HIV
    Steven Douglas; Fiscal Year: 2003
    ..These studies may provide new mechanistic insights related to the role of alcohol in the pathogenesis of HIV disease and AIDS and toward design of new therapeutic approaches. ..
  11. TACHYKININS, MONONUCLEAR PHAGOCYTES AND HIV1 INFECTION
    Steven Douglas; Fiscal Year: 1999
    ....
  12. TACHYKININS, MONONUCLEAR PHAGOCYTES AND HIV1 INFECTION
    Steven Douglas; Fiscal Year: 2000
    ....
  13. TACHYKININS, MONONUCLEAR PHAGOCYTES AND HIV1 INFECTION
    Steven Douglas; Fiscal Year: 2001
    ....
  14. Interaction of Human Immune Cells, Alcohol & HIV
    Steven Douglas; Fiscal Year: 2001
    ..These studies may provide new mechanistic insights related to the role of alcohol in the pathogenesis of HIV disease and AIDS and toward design of new therapeutic approaches. ..
  15. Interaction of Human Immune Cells Alcohol & HIV
    Steven Douglas; Fiscal Year: 2002
    ..These studies may provide new mechanistic insights related to the role of alcohol in the pathogenesis of HIV disease and AIDS and toward design of new therapeutic approaches. ..
  16. TACHYKININS, MONONUCLEAR PHAGOCYTES AND HIV1 INFECTION
    Steven Douglas; Fiscal Year: 2002
    ....
  17. Tachykinins Mononuclear Phagocytes and HIV-1 Infection
    Steven Douglas; Fiscal Year: 2003
    ..These proposed studies are a unique series of experiments directed toward elucidating the mechanistic role of SP and the SP receptor in the crucial links between psychosocial factors, depression and HIV/AIDS disease progression. ..
  18. Interaction of Human Immune Cells, Alcohol & HIV
    Steven Douglas; Fiscal Year: 2002
    ..These studies may provide new mechanistic insights related to the role of alcohol in the pathogenesis of HIV disease and AIDS and toward design of new therapeutic approaches. ..
  19. Tachykinins Mononuclear Phagocytes and HIV-1 Infection
    STEVEN DANIEL DOUGLAS; Fiscal Year: 2010
    ..Relevance to Public Health: These studies will further lead to understanding the pathogenesis of neurocognitive changes in HIV disease and lead to unique and novel therapeutic intervention. ..