Appolinaire Djikeng

Summary

Affiliation: The Institute for Genomic Research
Country: USA

Publications

  1. ncbi Cofactor-independent phosphoglycerate mutase is an essential gene in procyclic form Trypanosoma brucei
    Appolinaire Djikeng
    The Institute for Genomic Research TIGR, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Parasitol Res 100:887-92. 2007
  2. ncbi Comparative genomics of trypanosomatid parasitic protozoa
    Najib M El-Sayed
    Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Science 309:404-9. 2005
  3. pmc Viral genome sequencing by random priming methods
    Appolinaire Djikeng
    Viral Genomics Group, J, Craig Venter Institute, Rockville, MD 20850, USA
    BMC Genomics 9:5. 2008
  4. ncbi The genome of the African trypanosome Trypanosoma brucei
    Matthew Berriman
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton CB10 1SA, UK
    Science 309:416-22. 2005
  5. pmc RNA interference: advances and questions
    Elisabetta Ullu
    Department of Internal Medicine, and Department of Cell Biology, Yale Medical School, 333 Cedar Street, New Haven, CT 06520 8022, USA
    Philos Trans R Soc Lond B Biol Sci 357:65-70. 2002
  6. ncbi Analysis of gene function in Trypanosoma brucei using RNA interference
    Appolinaire Djikeng
    Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, USA
    Methods Mol Biol 265:73-83. 2004
  7. pmc Argonaute protein in the early divergent eukaryote Trypanosoma brucei: control of small interfering RNA accumulation and retroposon transcript abundance
    Huafang Shi
    Department of Internal Medicine, Yale University Medical School, New Haven, Connecticut 06536 0812, USA
    Mol Cell Biol 24:420-7. 2004
  8. ncbi In vivo analysis of the RNA interference mechanism in Trypanosoma brucei
    Christian Tschudi
    Department of Internal Medicine, Yale University Medical School, 333 Cedar Street, New Haven, Connecticut 06520 8022, USA
    Methods 30:304-12. 2003
  9. ncbi Repression of gene expression by the coliphage MS2 coat protein in Trypanosoma brucei
    Huafang Shi
    Department of Internal Medicine, Yale Medical School, BCMM 136D, 295 Congress Avenue, Box 9812, New Haven, CT 06536 8012, USA
    Mol Biochem Parasitol 144:119-22. 2005
  10. pmc An siRNA ribonucleoprotein is found associated with polyribosomes in Trypanosoma brucei
    Appolinaire Djikeng
    Department of Internal Medicine, Yale University Medical School, New Haven, Connecticut 06520 8022, USA
    RNA 9:802-8. 2003

Collaborators

Detail Information

Publications11

  1. ncbi Cofactor-independent phosphoglycerate mutase is an essential gene in procyclic form Trypanosoma brucei
    Appolinaire Djikeng
    The Institute for Genomic Research TIGR, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Parasitol Res 100:887-92. 2007
    ..We first demonstrated biochemically that depletion of the steady state levels of iPGM mRNA correlates with a marked reduction of enzyme activity. We further show that iPGAM is required for cell growth in procyclic T. brucei...
  2. ncbi Comparative genomics of trypanosomatid parasitic protozoa
    Najib M El-Sayed
    Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Science 309:404-9. 2005
    ..Contrary to recent reports, our analyses reveal no evidence that these species are descended from an ancestor that contained a photosynthetic endosymbiont...
  3. pmc Viral genome sequencing by random priming methods
    Appolinaire Djikeng
    Viral Genomics Group, J, Craig Venter Institute, Rockville, MD 20850, USA
    BMC Genomics 9:5. 2008
    ..Of increasing importance therefore is a better understanding of global viral diversity to enable better surveillance and prediction of pandemic threats; this will require rapid and flexible methods for complete viral genome sequencing...
  4. ncbi The genome of the African trypanosome Trypanosoma brucei
    Matthew Berriman
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton CB10 1SA, UK
    Science 309:416-22. 2005
    ..brucei and the greatest in L. major. Horizontal transfer of genes of bacterial origin has contributed to some of the metabolic differences in these parasites, and a number of novel potential drug targets have been identified...
  5. pmc RNA interference: advances and questions
    Elisabetta Ullu
    Department of Internal Medicine, and Department of Cell Biology, Yale Medical School, 333 Cedar Street, New Haven, CT 06520 8022, USA
    Philos Trans R Soc Lond B Biol Sci 357:65-70. 2002
    ..We provide a synopsis of what is currently known about RNAi in T. brucei and outline the recent advances that make RNAi the method of choice to disrupt gene function in these organisms...
  6. ncbi Analysis of gene function in Trypanosoma brucei using RNA interference
    Appolinaire Djikeng
    Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, USA
    Methods Mol Biol 265:73-83. 2004
    ..brucei with particular emphasis on transfection and cloning of procyclic cells, induction of dsRNA expression, isolation of RNA and analysis of dsRNA, and target mRNA...
  7. pmc Argonaute protein in the early divergent eukaryote Trypanosoma brucei: control of small interfering RNA accumulation and retroposon transcript abundance
    Huafang Shi
    Department of Internal Medicine, Yale University Medical School, New Haven, Connecticut 06536 0812, USA
    Mol Cell Biol 24:420-7. 2004
    ..Our results suggest that AGO1 function is required for production and/or stabilization of siRNAs and provide the first evidence for an Argonaute protein being involved in the regulation of retroposon transcript levels...
  8. ncbi In vivo analysis of the RNA interference mechanism in Trypanosoma brucei
    Christian Tschudi
    Department of Internal Medicine, Yale University Medical School, 333 Cedar Street, New Haven, Connecticut 06520 8022, USA
    Methods 30:304-12. 2003
    ..In this article, we describe some of the methods we employ for the analysis of the RNAi mechanism in T. brucei with particular emphasis on detection, cloning, and fractionation of siRNAs and siRNA complexes...
  9. ncbi Repression of gene expression by the coliphage MS2 coat protein in Trypanosoma brucei
    Huafang Shi
    Department of Internal Medicine, Yale Medical School, BCMM 136D, 295 Congress Avenue, Box 9812, New Haven, CT 06536 8012, USA
    Mol Biochem Parasitol 144:119-22. 2005
  10. pmc An siRNA ribonucleoprotein is found associated with polyribosomes in Trypanosoma brucei
    Appolinaire Djikeng
    Department of Internal Medicine, Yale University Medical School, New Haven, Connecticut 06520 8022, USA
    RNA 9:802-8. 2003
    ..We further provide evidence that siRNAs are associated with translating ribosomes and that this association is mediated by a 70-kD ribonucleoprotein complex...
  11. ncbi Analysis of gene function in Trypanosoma brucei using RNA interference
    Appolinaire Djikeng
    Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, USA
    Methods Mol Biol 270:287-98. 2004
    ..brucei with particular emphasis on transfection and cloning of procyclic cells, induction of dsRNA expression, isolation of RNA, and analysis of dsRNA and target mRNA...