Robert Darnell

Summary

Affiliation: The Rockefeller University
Country: USA

Publications

  1. pmc HITS-CLIP: panoramic views of protein-RNA regulation in living cells
    Robert B Darnell
    Laboratory of Neuro Oncology, The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10065, USA
    Wiley Interdiscip Rev RNA 1:266-86. 2010
  2. doi request reprint RNA protein interaction in neurons
    Robert B Darnell
    Department of Molecular Neuro Oncology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10065, USA
    Annu Rev Neurosci 36:243-70. 2013
  3. ncbi request reprint Paraneoplastic syndromes affecting the nervous system
    Robert B Darnell
    Howard Hughes Medical Institute and Laboratory of Molecular Neuro Oncology, The Rockefeller University, New York, NY 10021, USA
    Semin Oncol 33:270-98. 2006
  4. ncbi request reprint Neuronal signaling through alternative splicing: some exons CaRRE
    K J O'Donovan
    Laboratory of Molecular Neuro Oncology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Sci STKE 2001:pe2. 2001
  5. pmc Nova regulates GABA(A) receptor gamma2 alternative splicing via a distal downstream UCAU-rich intronic splicing enhancer
    B Kate Dredge
    Laboratory of Molecular Neuro Oncology, Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, USA
    Mol Cell Biol 23:4687-700. 2003
  6. ncbi request reprint An RNA map predicting Nova-dependent splicing regulation
    Jernej Ule
    Howard Hughes Medical Institute, New York, New York 10021, USA
    Nature 444:580-6. 2006
  7. ncbi request reprint CLIP identifies Nova-regulated RNA networks in the brain
    Jernej Ule
    Howard Hughes Medical Institute, Rockefeller University, New York, NY 10021, USA
    Science 302:1212-5. 2003
  8. pmc Nova autoregulation reveals dual functions in neuronal splicing
    B Kate Dredge
    Laboratory of Molecular Neuro Oncology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA
    EMBO J 24:1608-20. 2005
  9. pmc Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes
    Jennifer C Darnell
    Howard Hughes Medical Institute and Laboratory of Molecular Neuro Oncology, The Rockefeller University, New York, New York, USA
    Genes Dev 19:903-18. 2005
  10. ncbi request reprint Alternative splicing microarrays reveal functional expression of neuron-specific regulators in Hodgkin lymphoma cells
    Angela Relógio
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    J Biol Chem 280:4779-84. 2005

Research Grants

  1. Tolerance versus tumor immunity in PND
    Robert Darnell; Fiscal Year: 2005
  2. Structure/function studies of RBPs in neurologic disease
    Robert Darnell; Fiscal Year: 2005
  3. NEUROLOGY AND THE MOLECULAR ROLE OF N-RBPS IN THE BRAIN
    Robert Darnell; Fiscal Year: 2006
  4. Prostate Cancer Immunotherapy
    Robert Darnell; Fiscal Year: 2007
  5. NEUROLOGY AND THE MOLECULAR ROLE OF N-RBPS IN THE BRAIN
    Robert Darnell; Fiscal Year: 2009
  6. NEUROLOGY AND THE MOLECULAR ROLE OF N-RBPS IN THE BRAIN
    Robert Darnell; Fiscal Year: 2001
  7. NEUROLOGY AND THE MOLECULAR ROLE OF N-RBPS IN THE BRAIN
    Robert B Darnell; Fiscal Year: 2010

Collaborators

  • B J Blencowe
  • Jerome Posner
  • Bahar Taneri
  • K Musunuru
  • H Okano
  • William T O'donnell
  • S R Eddy
  • CHARLES M contact RICE
  • JENNIFER DARNELL
  • Jernej Ule
  • Matteo Ruggiu
  • Giovanni Stefani
  • B Kate Dredge
  • Wendy K Roberts
  • Aldo Mele
  • Bianca D Santomasso
  • Nejc Jelen
  • Randy S Longman
  • Donny D Licatalosi
  • Cindy Shen Huang
  • Angela Relógio
  • Aljaz Ule
  • Hiroyuki Ohba
  • K J O'Donovan
  • Sandra Pellegrini
  • Mark E Dudley
  • Travis Williams
  • Deborah Braun
  • Ashby Thomas
  • Alan N Houghton
  • Matthew L Albert
  • Nathalie E Blachere
  • Marko Zivin
  • Terry Gaasterland
  • Xuning Wang
  • John N Weinstein
  • David Kane
  • Juan Valcarcel
  • Joanna Spencer
  • Jing Shan Hu
  • Alan Williams
  • Hui Wang
  • Laura A Barker
  • Lily Yeh Jan
  • Claire Fraser
  • Vladimir Benes
  • Kirk Jensen
  • Yuh Nung Jan
  • John Blume
  • Melissa Cline
  • Tyson Clark
  • Christine Gemund
  • Claudia Ben-Dov
  • Song Hai Shi
  • Barry R Zeeberg
  • Michael Baum
  • Caitlin C Engelhard
  • Emmy Endo
  • Claire E Fraser
  • Masanori Sakaguchi
  • Masato Yano
  • Tatsuyuki Chiyoda
  • Yoshika Hayakawa
  • Kirk B Jensen

Detail Information

Publications28

  1. pmc HITS-CLIP: panoramic views of protein-RNA regulation in living cells
    Robert B Darnell
    Laboratory of Neuro Oncology, The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10065, USA
    Wiley Interdiscip Rev RNA 1:266-86. 2010
    ..Examples of the successes, limitations, and future of the technique will be discussed...
  2. doi request reprint RNA protein interaction in neurons
    Robert B Darnell
    Department of Molecular Neuro Oncology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10065, USA
    Annu Rev Neurosci 36:243-70. 2013
    ..Here we review what is known about such RNABPs and explore the potential biologic and neurologic significance of neuronal RNA regulatory systems. ..
  3. ncbi request reprint Paraneoplastic syndromes affecting the nervous system
    Robert B Darnell
    Howard Hughes Medical Institute and Laboratory of Molecular Neuro Oncology, The Rockefeller University, New York, NY 10021, USA
    Semin Oncol 33:270-98. 2006
    ..We review the currently known PND and their pathogenesis...
  4. ncbi request reprint Neuronal signaling through alternative splicing: some exons CaRRE
    K J O'Donovan
    Laboratory of Molecular Neuro Oncology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Sci STKE 2001:pe2. 2001
    ..O'Donovan and Darnell discuss a mechanism by which neurons can alter channel splicing in response to neuronal activity through a signal generated by calcium and calcium/calmodulin-dependent kinase activity...
  5. pmc Nova regulates GABA(A) receptor gamma2 alternative splicing via a distal downstream UCAU-rich intronic splicing enhancer
    B Kate Dredge
    Laboratory of Molecular Neuro Oncology, Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, USA
    Mol Cell Biol 23:4687-700. 2003
    ....
  6. ncbi request reprint An RNA map predicting Nova-dependent splicing regulation
    Jernej Ule
    Howard Hughes Medical Institute, New York, New York 10021, USA
    Nature 444:580-6. 2006
    ....
  7. ncbi request reprint CLIP identifies Nova-regulated RNA networks in the brain
    Jernej Ule
    Howard Hughes Medical Institute, Rockefeller University, New York, NY 10021, USA
    Science 302:1212-5. 2003
    ..Thus, CLIP reveals that Nova coordinately regulates a biologically coherent set of RNAs encoding multiple components of the inhibitory synapse, an observation that may relate to the cause of abnormal motor inhibition in POMA...
  8. pmc Nova autoregulation reveals dual functions in neuronal splicing
    B Kate Dredge
    Laboratory of Molecular Neuro Oncology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA
    EMBO J 24:1608-20. 2005
    ..These studies demonstrate that in addition to its previously described role as a splicing activator, Nova autoregulates its own expression by acting as a splicing repressor...
  9. pmc Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes
    Jennifer C Darnell
    Howard Hughes Medical Institute and Laboratory of Molecular Neuro Oncology, The Rockefeller University, New York, New York, USA
    Genes Dev 19:903-18. 2005
    ....
  10. ncbi request reprint Alternative splicing microarrays reveal functional expression of neuron-specific regulators in Hodgkin lymphoma cells
    Angela Relógio
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    J Biol Chem 280:4779-84. 2005
    ..This microarray design can help assess the role of alternative splicing in a variety of biological and medical problems and potentially serve as a diagnostic tool...
  11. pmc Determination and augmentation of RNA sequence specificity of the Nova K-homology domains
    Kiran Musunuru
    Laboratory of Molecular Neuro Oncology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Nucleic Acids Res 32:4852-61. 2004
    ..These data yield important insights into KH domain function and suggest a strategy by which to engineer KH domains with novel sequence preferences...
  12. ncbi request reprint Fragile X mental retardation protein is associated with translating polyribosomes in neuronal cells
    Giovanni Stefani
    Howard Hughes Medical Institute and Laboratory of Molecular Neuro Oncology, The Rockefeller University, New York, New York 10021, USA
    J Neurosci 24:7272-6. 2004
    ..These data indicate that FMRP associates with functional polyribosomes in neurons...
  13. ncbi request reprint RNA logic in time and space
    Robert B Darnell
    Howard Hughes Medical Institute, Laboratory of Molecular Neuro Oncology, The Rockefeller University, New York, NY 10021, USA
    Cell 110:545-50. 2002
    ..The talks covered mechanisms of translational regulation, particularly in terms of temporal and spatial regulation, over a wide range of biological systems...
  14. ncbi request reprint The fragile X mental retardation protein, FMRP, recognizes G-quartets
    Jennifer C Darnell
    The Rockefeller University, Laboratory of Molecular Neuro Oncology New York, New York 10021, USA
    Ment Retard Dev Disabil Res Rev 10:49-52. 2004
    ..Defects in the metabolism of this set of RNAs, presumably in the translation of their protein products, is likely to underlie the behavioral and cognitive changes seen in the disease...
  15. ncbi request reprint Paraneoplastic neurologic disorders: windows into neuronal function and tumor immunity
    Robert B Darnell
    Department of Molecular Neuro Oncology, Howard Hughes Medical Institute, Rockefeller University, Box 226, 1230 York Avenue, New York, NY 10021 6399, USA
    Arch Neurol 61:30-2. 2004
  16. ncbi request reprint Functional and mechanistic insights from genome-wide studies of splicing regulation in the brain
    Jernej Ule
    MRC Laboratory of Molecular Biology, Cambridge, England
    Adv Exp Med Biol 623:148-60. 2007
    ..These studies provide us with a platform for understanding the role of RNA binding proteins in tissue-specific splicing regulation and in disease...
  17. pmc A T-cell receptor associated with naturally occurring human tumor immunity
    Bianca D Santomasso
    Howard Hughes Medical Institute, Laboratory of Molecular Neuro Oncology, The Rockefeller University, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 104:19073-8. 2007
    ..Cloned cdr2-specific TCR genes provide a clinically relevant means for immunologic targeting of human gynecologic cancers...
  18. pmc Evolution of Nova-dependent splicing regulation in the brain
    Nejc Jelen
    Laboratory of Molecular Neuro Oncology, The Rockefeller University, New York, New York, USA
    PLoS Genet 3:1838-47. 2007
    ....
  19. ncbi request reprint Dendritic-cell maturation alters intracellular signaling networks, enabling differential effects of IFN-alpha/beta on antigen cross-presentation
    Randy S Longman
    Rockefeller University, New York, NY, USA
    Blood 109:1113-22. 2007
    ..These data provide evidence for a molecular switch in signaling pathways concomitant with DC maturation that offers a novel mechanism by which DCs modulate the integration of signals from the surrounding environment...
  20. ncbi request reprint Memory, synaptic translation, and...prions?
    Robert B Darnell
    Howard Hughes Medical Institute, Laboratory of Molecular Neuro Oncology, The Rockefeller University, New York, NY 10021, USA
    Cell 115:767-8. 2003
    ..2003a, 2003b [this issue of Cell])...
  21. ncbi request reprint Splicing regulation in neurologic disease
    Donny D Licatalosi
    Howard Hughes Medical Institute, Laboratory of Molecular Neuro Oncology, The Rockefeller University, New York, New York 10021, USA
    Neuron 52:93-101. 2006
    ..This review considers the evidence that defects in regulation of splicing may underlie many types of human neurologic diseases...
  22. ncbi request reprint RNA binding proteins and the regulation of neuronal synaptic plasticity
    Jernej Ule
    Howard Hughes Medical Institute and the Laboratory of Molecular Neuro Oncology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Curr Opin Neurobiol 16:102-10. 2006
    ..Specificity in the regulation of synaptic plasticity might, in part, relate to the functional coherence of proteins encoded by the RNA targets of each RNA-binding protein...
  23. ncbi request reprint CLIP: a method for identifying protein-RNA interaction sites in living cells
    Jernej Ule
    Howard Hughes Medical Institute and the Laboratory of Molecular Neuro Oncology, The Rockefeller University, New York, USA
    Methods 37:376-86. 2005
    ..Here, we present an improved protocol that performs RNA linker ligation before the SDS-PAGE step, and describe its application to the specific purification and amplification of RNA ligands of Nova in neurons...
  24. ncbi request reprint Common molecular pathways mediate long-term potentiation of synaptic excitation and slow synaptic inhibition
    Cindy Shen Huang
    Howard Hughes Medical Institute and Departments of Physiology and Biochemistry, University of California, San Francisco, CA 94143, USA
    Cell 123:105-18. 2005
    ....
  25. ncbi request reprint Nova regulates brain-specific splicing to shape the synapse
    Jernej Ule
    Howard Hughes Medical Institute and Laboratory of Molecular Neuro Oncology, The Rockefeller University, New York, New York, USA
    Nat Genet 37:844-52. 2005
    ..Validating a large set of Nova RNA targets has led us to identify a multi-tiered network in which Nova regulates the exon content of RNAs encoding proteins that interact in the synapse...
  26. ncbi request reprint Neuroimmunology of the paraneoplastic neurological degenerations
    Wendy K Roberts
    Howard Hughes Medical Institute, The Rockefeller University, Box 226, 1230 York Avenue, New York, New York 10021, USA
    Curr Opin Immunol 16:616-22. 2004
    ..Pieces of the mystery of this tumor immune response and neuronal autoimmunity have been assembled, but much more needs to be learned...
  27. ncbi request reprint Sox21 is a repressor of neuronal differentiation and is antagonized by YB-1
    Hiroyuki Ohba
    CREST JST and Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    Neurosci Lett 358:157-60. 2004
    ..These results suggest that Sox21 is a repressor of neuronal differentiation in the developing nervous system...
  28. ncbi request reprint A new cause of limbic encephalopathy
    Robert B Darnell
    The Rockefeller University, New York, USA
    Brain 128:1745-6. 2005

Research Grants32

  1. Tolerance versus tumor immunity in PND
    Robert Darnell; Fiscal Year: 2005
    ..These experiments offer the possibility of developing new cancer treatments and advancing the understanding of autoimmune disease of the nervous system. ..
  2. Structure/function studies of RBPs in neurologic disease
    Robert Darnell; Fiscal Year: 2005
    ....
  3. NEUROLOGY AND THE MOLECULAR ROLE OF N-RBPS IN THE BRAIN
    Robert Darnell; Fiscal Year: 2006
    ..These studies will allow us to formulate a model comparing and contrasting the role that the Nova and FMRP RNA binding proteins in neurons and in neurologic disease. ..
  4. Prostate Cancer Immunotherapy
    Robert Darnell; Fiscal Year: 2007
    ..Our results should indicate whether this new approach to DC based, immunotherapy has a potential role in the treatment of prostatic cancer as well as other malignancies. ..
  5. NEUROLOGY AND THE MOLECULAR ROLE OF N-RBPS IN THE BRAIN
    Robert Darnell; Fiscal Year: 2009
    ..abstract_text> ..
  6. NEUROLOGY AND THE MOLECULAR ROLE OF N-RBPS IN THE BRAIN
    Robert Darnell; Fiscal Year: 2001
    ....
  7. NEUROLOGY AND THE MOLECULAR ROLE OF N-RBPS IN THE BRAIN
    Robert B Darnell; Fiscal Year: 2010
    ....