G Danuser

Summary

Affiliation: The Scripps Research Institute
Country: USA

Publications

  1. ncbi request reprint New directions for fluorescent speckle microscopy
    Clare M Waterman-Storer
    Department of Cell Biology and Institute for Childhood and Neglected Diseases, The Scripps Research Institute, La Jolla, CA 92037, USA
    Curr Biol 12:R633-40. 2002
  2. ncbi request reprint Computational processing and analysis of dynamic fluorescence image data
    Jonas F Dorn
    Laboratory for Computational Cell Biology, Department of Cell Biology, CB167 The Scripps Research Institute La Jolla, California 92037, USA
    Methods Cell Biol 85:497-538. 2008
  3. pmc Fluctuations of intracellular forces during cell protrusion
    Lin Ji
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Nat Cell Biol 10:1393-400. 2008
  4. ncbi request reprint Quantitative fluorescent speckle microscopy of cytoskeleton dynamics
    Gaudenz Danuser
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Annu Rev Biophys Biomol Struct 35:361-87. 2006
  5. ncbi request reprint Coupling the dynamics of two actin networks--new views on the mechanics of cell protrusion
    G Danuser
    The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Biochem Soc Trans 33:1250-3. 2005
  6. ncbi request reprint Quantitative fluorescent speckle microscopy: where it came from and where it is going
    G Danuser
    BioMicroMetrics Group, Laboratory for Biomechanics, ETH Zurich, 8952 Schlieren, Switzerland
    J Microsc 211:191-207. 2003
  7. pmc Periodic patterns of actin turnover in lamellipodia and lamellae of migrating epithelial cells analyzed by quantitative Fluorescent Speckle Microscopy
    A Ponti
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California, USA
    Biophys J 89:3456-69. 2005
  8. pmc Recovery, visualization, and analysis of actin and tubulin polymer flow in live cells: a fluorescent speckle microscopy study
    P Vallotton
    BioMicroMetrics Group, Laboratory for Biomechanics, ETH Zurich, 8952 Schlieren, Switzerland
    Biophys J 85:1289-306. 2003
  9. ncbi request reprint Signal analysis of total internal reflection fluorescent speckle microscopy (TIR-FSM) and wide-field epi-fluorescence FSM of the actin cytoskeleton and focal adhesions in living cells
    M C Adams
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Microsc 216:138-52. 2004
  10. ncbi request reprint Two distinct actin networks drive the protrusion of migrating cells
    A Ponti
    Department of Cell Biology, Scripps Research Institute TSRI, La Jolla, CA 92037, USA
    Science 305:1782-6. 2004

Research Grants

  1. The Mechanics of Actin-mediated Cell Protrusion
    Gaudenz Danuser; Fiscal Year: 2007

Collaborators

Detail Information

Publications41

  1. ncbi request reprint New directions for fluorescent speckle microscopy
    Clare M Waterman-Storer
    Department of Cell Biology and Institute for Childhood and Neglected Diseases, The Scripps Research Institute, La Jolla, CA 92037, USA
    Curr Biol 12:R633-40. 2002
    ..Here, we review the most recent applications and developments and give a glimpse of future directions and potentials of FSM...
  2. ncbi request reprint Computational processing and analysis of dynamic fluorescence image data
    Jonas F Dorn
    Laboratory for Computational Cell Biology, Department of Cell Biology, CB167 The Scripps Research Institute La Jolla, California 92037, USA
    Methods Cell Biol 85:497-538. 2008
    ..It also aims to introduce the terminology and central concepts of computer vision to facilitate the communication between cell biologists and computer scientists in collaborative imaging projects...
  3. pmc Fluctuations of intracellular forces during cell protrusion
    Lin Ji
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Nat Cell Biol 10:1393-400. 2008
    ....
  4. ncbi request reprint Quantitative fluorescent speckle microscopy of cytoskeleton dynamics
    Gaudenz Danuser
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Annu Rev Biophys Biomol Struct 35:361-87. 2006
    ....
  5. ncbi request reprint Coupling the dynamics of two actin networks--new views on the mechanics of cell protrusion
    G Danuser
    The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Biochem Soc Trans 33:1250-3. 2005
    ....
  6. ncbi request reprint Quantitative fluorescent speckle microscopy: where it came from and where it is going
    G Danuser
    BioMicroMetrics Group, Laboratory for Biomechanics, ETH Zurich, 8952 Schlieren, Switzerland
    J Microsc 211:191-207. 2003
    ..Biol., 12, R633-R640), in which we emphasized the use of FSM in cell biological applications. Here, we focus on the technical aspects of making FSM a quantitative method...
  7. pmc Periodic patterns of actin turnover in lamellipodia and lamellae of migrating epithelial cells analyzed by quantitative Fluorescent Speckle Microscopy
    A Ponti
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California, USA
    Biophys J 89:3456-69. 2005
    ..Several algorithmic advances in speckle image processing are described enabling the analysis of kinetic and kinematic activities of polymer networks at the level of single speckles...
  8. pmc Recovery, visualization, and analysis of actin and tubulin polymer flow in live cells: a fluorescent speckle microscopy study
    P Vallotton
    BioMicroMetrics Group, Laboratory for Biomechanics, ETH Zurich, 8952 Schlieren, Switzerland
    Biophys J 85:1289-306. 2003
    ..Third, we analyze microtubule poleward flux in mitotic metaphase spindles assembled in Xenopus egg extracts, bringing new insight into the dynamics of microtubule assemblies in this system...
  9. ncbi request reprint Signal analysis of total internal reflection fluorescent speckle microscopy (TIR-FSM) and wide-field epi-fluorescence FSM of the actin cytoskeleton and focal adhesions in living cells
    M C Adams
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Microsc 216:138-52. 2004
    ..When used in time-lapse mode, TIR-FSM of actin and GFP-conjugated focal adhesion proteins will allow quantification of molecular dynamics within interesting macromolecular assemblies at the ventral surface of living cells...
  10. ncbi request reprint Two distinct actin networks drive the protrusion of migrating cells
    A Ponti
    Department of Cell Biology, Scripps Research Institute TSRI, La Jolla, CA 92037, USA
    Science 305:1782-6. 2004
    ..Productive cell advance was a function of the second colocalized network, the lamella, where actomyosin contraction was integrated with substrate adhesion...
  11. pmc Yeast kinetochore microtubule dynamics analyzed by high-resolution three-dimensional microscopy
    J F Dorn
    Laboratory for Computational Cell Biology, Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Biophys J 89:2835-54. 2005
    ..Based on these findings, we propose high-resolution light microscopy of centromere dynamics in G1 yeast cells as a sensitive assay for the regulation of single k-MT dynamics...
  12. ncbi request reprint Phenotypic clustering of yeast mutants based on kinetochore microtubule dynamics
    K Jaqaman
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Bioinformatics 23:1666-73. 2007
    ..Our goal is to identify groups of kinetochore proteins with similar effects on MT dynamics, revealing pathways through which kinetochore proteins transform chemical and mechanical input signals into cues of MT regulation...
  13. pmc Probing f-actin flow by tracking shape fluctuations of radial bundles in lamellipodia of motile cells
    G Danuser
    Department of Materials, ETH Zurich, Schlieren, Switzerland
    Biophys J 79:191-201. 2000
    ..We have generated a detailed map of the complex retrograde flow pattern throughout the lamellipodium. Such two-dimensional flow maps will give new insights into the mechanisms responsible for f-actin-mediated cell motility and growth...
  14. pmc Morphodynamic profiling of protrusion phenotypes
    M Machacek
    Laboratory for Computational Cell Biology, Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Biophys J 90:1439-52. 2006
    ..Our data support a model where activation of Rac1 mediates the propagation of protrusion waves, whose persistence depends on the relative abundance of activated Arp2/3 and polymerizable G-actin...
  15. ncbi request reprint Tracking quasi-stationary flow of weak fluorescent signals by adaptive multi-frame correlation
    L Ji
    Laboratory for Computational Cell Biology, Department of Cell Biology, CB167, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    J Microsc 220:150-67. 2005
    ..Thus, we can now probe cytoskeleton polymer dynamics in living cells at an entirely new level of complexity and with unprecedented detail...
  16. pmc Computational analysis of F-actin turnover in cortical actin meshworks using fluorescent speckle microscopy
    A Ponti
    BioMicroMetrics Group, Laboratory for Biomechanics, ETH Zurich, 8952 Schlieren, Switzerland
    Biophys J 84:3336-52. 2003
    ....
  17. ncbi request reprint Automatic fluorescent tag localization II: Improvement in super-resolution by relative tracking
    D Thomann
    Bio Micro Metrics Group, Laboratory for Biomechanics, Swiss Federal Institute of Technology, Wagistrasse 4, CH 8952 Schlieren, Switzerland
    J Microsc 211:230-48. 2003
    ..We have applied the new tracking system to extract metaphase trajectories of fluorescently tagged chromosomes relative to the spindle poles in budding yeast...
  18. ncbi request reprint Surface modification of PLGA microspheres
    M Muller
    Laboratory for Surface Science and Technology, Department of Materials, Swiss Federal Institute of Technology, ETH Zurich, Switzerland
    J Biomed Mater Res A 66:55-61. 2003
    ..Low protein-binding, functionalizable microspheres provide a fundamental basis for the design of drug delivery and biosensor systems...
  19. ncbi request reprint Automatic fluorescent tag detection in 3D with super-resolution: application to the analysis of chromosome movement
    D Thomann
    Bio Micro Metrics Group, Laboratory for Biomechanics, Swiss Federal Institute of Technology, Wagistrasse 4, CH 8952 Schlieren, Switzerland
    J Microsc 208:49-64. 2002
    ..This indicates the intimate relationship between resolution and localization accuracy...
  20. pmc Myosin II activity facilitates microtubule bundling in the neuronal growth cone neck
    Dylan T Burnette
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06511, USA
    Dev Cell 15:163-9. 2008
    ..Upon Myosin II inhibition, the movement of actin filaments and MTs immediately stopped and MTs unbundled. Thus, Myosin II-dependent compressive force is necessary for normal MT bundling in the growth cone neck...
  21. ncbi request reprint Probing intracellular force distributions by high-resolution live cell imaging and inverse dynamics
    Lin Ji
    The Scripps Research Institute, La Jolla, California 92037, USA
    Methods Cell Biol 83:199-235. 2007
    ..This technique will potentially allow the analysis of intracellular force regulation in numerous other cell functions...
  22. ncbi request reprint Locomotion of fish epidermal keratocytes on spatially selective adhesion patterns
    Gabor Csucs
    Laboratory for Biomechanics, Department of Mechanical Engineering, ETH Zurich, 8952 Schlieren, Switzerland
    Cell Motil Cytoskeleton 64:856-67. 2007
    ..This study establishes spatially selective adhesion substrates and cell morphological readouts as a means to elucidate the mechanical balance between substrate adhesion and cytoskeleton-internal tension in cell migration...
  23. pmc Actin-myosin network reorganization breaks symmetry at the cell rear to spontaneously initiate polarized cell motility
    Patricia T Yam
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Cell Biol 178:1207-21. 2007
    ..Together, these results indicate that large-scale actin-myosin network reorganization and contractility at the cell rear initiate spontaneous symmetry breaking and polarized motility of keratocytes...
  24. ncbi request reprint Architectural dynamics of the meiotic spindle revealed by single-fluorophore imaging
    Ge Yang
    Laboratory for Computational Cell Biology, Scripps Research Institute, 10550 North Torrey Pines Road, Mail Box CB167, La Jolla, CA 92037, USA
    Nat Cell Biol 9:1233-42. 2007
    ..Our data suggest that force transmission within the spindle must be understood in terms of the crosslinking dynamics of a tiled array of individual filaments, most of which do not span the distance from the pole to the metaphase plate...
  25. pmc Cofilin activity downstream of Pak1 regulates cell protrusion efficiency by organizing lamellipodium and lamella actin networks
    Violaine Delorme
    Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Dev Cell 13:646-62. 2007
    ..We propose that cofilin functions as a regulator of cell protrusion by modulating the spatial interaction of the lamellipodium and lamella in response to upstream signals...
  26. pmc Robust single-particle tracking in live-cell time-lapse sequences
    Khuloud Jaqaman
    Department of Cell Biology, The Scripps Research Institute, 10550 N Torrey Pines Rd, La Jolla, California 92037, USA
    Nat Methods 5:695-702. 2008
    ..Both applications indicate the requirement for robust and complete tracking of dense particle fields to dissect the mechanisms of receptor organization at the level of the plasma membrane...
  27. pmc Visualizing and quantifying adhesive signals
    Mohsen Sabouri-Ghomi
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Curr Opin Cell Biol 20:541-50. 2008
    ..We conclude by outlining computational multiplexing as a framework for the integration of these data into comprehensive models of adhesion signaling pathways...
  28. pmc Coordination of actin filament and microtubule dynamics during neurite outgrowth
    Andrew W Schaefer
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06511, USA
    Dev Cell 15:146-62. 2008
    ..These results reveal a role for Rho Kinase and myosin II contractility in regulation of microtubule behavior during neuronal growth...
  29. ncbi request reprint Differential transmission of actin motion within focal adhesions
    Ke Hu
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Science 315:111-5. 2007
    ....
  30. pmc Simultaneous mapping of filamentous actin flow and turnover in migrating cells by quantitative fluorescent speckle microscopy
    Pascal Vallotton
    BioMicroMetrics Group, Laboratory for Biomechanics, Swiss Federal Institute of Technology, 8952 Schlieren, Switzerland
    Proc Natl Acad Sci U S A 101:9660-5. 2004
    ..There appear to be two distinct depolymerization mechanisms, of which one depends directly on meshwork contraction...
  31. pmc Tracking retrograde flow in keratocytes: news from the front
    Pascal Vallotton
    Laboratory for Biomechanics, ETH Zurich, 8952 Schlieren, Switzerland
    Mol Biol Cell 16:1223-31. 2005
    ..Our findings support the universality of the flow phenomenon and indicate that the maintenance of keratocyte shape during locomotion depends on the regulation of both retrograde flow and actin polymerization...
  32. pmc Cell migration without a lamellipodium: translation of actin dynamics into cell movement mediated by tropomyosin
    Stephanie L Gupton
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Biol 168:619-31. 2005
    ..Inhibition of endogenous long TM isoforms alters protrusion persistence. Thus, cells can migrate with inhibited lamellipodia, and we suggest that TM is a major regulator of F-actin functional specialization in migrating cells...
  33. pmc Regional variation of microtubule flux reveals microtubule organization in the metaphase meiotic spindle
    Ge Yang
    Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Biol 182:631-9. 2008
    ....
  34. ncbi request reprint Microcontact printing of novel co-polymers in combination with proteins for cell-biological applications
    Gabor Csucs
    BioMicroMetricsGroup, BMMG, ETH Zurich, Wagistrasse 4, Schlieren CH 8952, Switzerland
    Biomaterials 24:1713-20. 2003
    ....
  35. pmc FRET or no FRET: a quantitative comparison
    Claude Berney
    BioMicroMetrics Group, Laboratory for Biomechanics, Swiss Federal Institute of Technology, CH 8952 Schlieren, Switzerland
    Biophys J 84:3992-4010. 2003
    ..Finally, we test their sensitivity and draw conclusions for the preparation of FRET experiments in more complex and less-controlled systems...
  36. pmc Integrin-dependent actomyosin contraction regulates epithelial cell scattering
    Johan de Rooij
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Cell Biol 171:153-64. 2005
    ..We conclude that integrin-dependent actomyosin traction force mediates the disruption of cell-cell adhesion during epithelial cell scattering...
  37. ncbi request reprint Filopodial actin bundles are not necessary for microtubule advance into the peripheral domain of Aplysia neuronal growth cones
    Dylan T Burnette
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06511, USA
    Nat Cell Biol 9:1360-9. 2007
    ..The resulting dynamic steady state has interesting implications for rapid microtubule-positioning responses in the P domain...
  38. pmc Kinesin 5-independent poleward flux of kinetochore microtubules in PtK1 cells
    Lisa A Cameron
    Department of Biology, University of North Carolina at Chapel Hill, 27599, USA
    J Cell Biol 173:173-9. 2006
    ..One candidate, Kif2a (kinesin 13), was detected at minus ends of fluxing kinetochore fibers. Kif2a remains associated with the ends of K fibers upon disruption of the spindle by dynein/dynactin inhibition, and these K fibers flux...
  39. pmc Comparative autoregressive moving average analysis of kinetochore microtubule dynamics in yeast
    Khuloud Jaqaman
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California
    Biophys J 91:2312-25. 2006
    ....
  40. ncbi request reprint Linking data to models: data regression
    Khuloud Jaqaman
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Rev Mol Cell Biol 7:813-9. 2006
    ....
  41. pmc Positional stability of single double-strand breaks in mammalian cells
    Evi Soutoglou
    National Cancer Institute, NIH, Bethesda, MD 20892, USA
    Nat Cell Biol 9:675-82. 2007
    ..These results support a contact-first model in which chromosome translocations predominantly form among spatially proximal DSBs...

Research Grants3

  1. The Mechanics of Actin-mediated Cell Protrusion
    Gaudenz Danuser; Fiscal Year: 2007
    ..Second, the non-steady state measurements provided by these methods will initiate novel numerical modelling to recapitulate the non-linear dynamics of cell protrusion. ..