Research Topics
Genomes and Genes | F R CrossSummaryAffiliation: The Rockefeller University Country: USA Publications
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Publications
Molecular evolution allows bypass of the requirement for activation loop phosphorylation of the Cdc28 cyclin-dependent kinaseF R Cross
The Rockefeller University, New York, New York 10021, USA
Mol Cell Biol 18:2923-31. 1998..Some growth defects remain in suppressed cak1 cdc28 strains carrying the mutant CDC28 genes, consistent with additional nonessential roles for CAK1...- Testing a mathematical model of the yeast cell cycleFrederick R Cross
The Rockefeller University, New York, NY 10021, USA
Mol Biol Cell 13:52-70. 2002..Thus, the model is a strong but incomplete attempt at a realistic representation of cell cycle control. Constraints of the sort developed here will be important in development of a truly predictive model... 
Specialization and targeting of B-type cyclinsF R Cross
Rockefeller University New York, New York 10021, USA
Mol Cell 4:11-9. 1999..Mutagenesis of a potential substrate-targeting domain of CLB5 reduced biological activity without reducing Clb5p-associated kinase activity. Thus, Clb5p may have targeting domains required for CLB5-specific biological activity...- Genetic analysis of the relationship between activation loop phosphorylation and cyclin binding in the activation of the Saccharomyces cerevisiae Cdc28p cyclin-dependent kinaseF R Cross
The Rockefeller University, New York, NY 10021, USA
Genetics 154:1549-59. 2000..This conclusion was supported by analysis of suppressors of a mutation in the Cdk phosphothreonine-binding pocket created by cyclin binding... - Conservation and function of a potential substrate-binding domain in the yeast Clb5 B-type cyclinF R Cross
The Rockefeller University, New York, NY 10021, USA
Mol Cell Biol 20:4782-90. 2000..These results support the idea of a modular, structurally conserved cyclin domain involved in substrate targeting... - Starting the cell cycle: what's the point?F R Cross
Rockefeller University, New York, USA
Curr Opin Cell Biol 7:790-7. 1995..Cln-Cdc28 kinases may directly regulate many cell cycle processes, including some classically considered to be 'post-Start'. Specialization of function among the three genetically redundant CLN genes is becoming apparent... - Mutations in SID2, a novel gene in Saccharomyces cerevisiae, cause synthetic lethality with sic1 deletion and may cause a defect during S phaseM D Jacobson
The Rockefeller University, New York, New York 10021, USA
Genetics 159:17-33. 2001..Consistent with this hypothesis, sid2-1 rad9 cells are dead or very slow growing even when SIC1 is expressed... - Mutations in CDC14 result in high sensitivity to cyclin gene dosage in Saccharomyces cerevisiaeM Yuste-Rojas
Rockefeller University, New York, NY 10021, USA
Mol Gen Genet 263:60-72. 2000..We also describe genetic interactions between CDC28 and CDC14... - Cyclin-specific START events and the G1-phase specificity of arrest by mating factor in budding yeastL J Oehlen
The Rockefeller University, New York, NY 10021, USA
Mol Gen Genet 258:183-98. 1998.... - Role of Swi4 in cell cycle regulation of CLN2 expressionF R Cross
Rockefeller University, New York, New York 10021
Mol Cell Biol 14:4779-87. 1994..Swi4 may bind to nonconsensus sequences in the CLN2 promoter (possibly in addition to consensus sites), or it may act indirectly to regulate CLN2 expression... - Genetic analysis of Cln/Cdc28 regulation of cell morphogenesis in budding yeastB K Benton
Rockefeller University, New York, NY 10021
EMBO J 12:5267-75. 1993..These results suggest direct regulation of diverse aspects of bud morphogenesis by Cln/Cdc28p activity... - Testing cyclin specificity in the exit from mitosisM D Jacobson
The Rockefeller University, New York, NY 10021, USA
Mol Cell Biol 20:4483-93. 2000.... - Distinct subcellular localization patterns contribute to functional specificity of the Cln2 and Cln3 cyclins of Saccharomyces cerevisiaeM E Miller
The Rockefeller University, New York, New York 10021, USA
Mol Cell Biol 20:542-55. 2000..The data presented here support the idea that cyclin function is regulated at the level of subcellular localization and that subcellular localization contributes to the functional specificity of Cln2p and Cln3p... - Directed evolution to bypass cyclin requirements for the Cdc28p cyclin-dependent kinaseK Levine
Rockefeller University, New York, New York, USA
Mol Cell 4:353-63. 1999..Thus, the mutants' CLN bypass activity might result from constitutive, cyclin-independent activity, suggesting that Cdk targeting by cyclins is not required for cell cycle initiation... - Genes that can bypass the CLN requirement for Saccharomyces cerevisiae cell cycle STARTC B Epstein
Rockefeller University, New York, New York 10021
Mol Cell Biol 14:2041-7. 1994..Thus, factors implicated in CLN1 and CLN2 expression and/or function are also required for BYC1 function in the absence of all three CLN genes; this may suggest the involvement of other targets of Swi4, Swi6, and Bck2 in START... - Cla4p, a Saccharomyces cerevisiae Cdc42p-activated kinase involved in cytokinesis, is activated at mitosisB K Benton
The Rockefeller University, New York, New York 10021, USA
Mol Cell Biol 17:5067-76. 1997..Cla4p kinase activity was not reduced by mutational inactivation of gin4, suggesting that Gin4p may be downstream or parallel to Cla4p in the regulation of cytokinesis... - Potential regulation of Ste20 function by the Cln1-Cdc28 and Cln2-Cdc28 cyclin-dependent protein kinasesL J Oehlen
Rockefeller University, New York, New York 10021, USA
J Biol Chem 273:25089-97. 1998.... - G1 cyclins CLN1 and CLN2 repress the mating factor response pathway at Start in the yeast cell cycleL J Oehlen
Rockefeller University, New York, New York 10021
Genes Dev 8:1058-70. 1994..The repression at Start of pheromone signaling by Cln1-Cdc28p or Cln2-Cdc28p kinase complexes may contribute to the acquisition of pheromone resistance as cells execute Start... - The role of Cdc42 in signal transduction and mating of the budding yeast Saccharomyces cerevisiaeL J Oehlen
Rockefeller University, New York, New York 10021, USA
J Biol Chem 273:8556-9. 1998..These results show that Cdc42 is not required for transduction of the mating factor signal per se but that it is essential for proper mating factor-induced morphogenesis... - The yeast Cln3 protein is an unstable activator of Cdc28F R Cross
Rockefeller University, New York, New York 10021
Mol Cell Biol 13:3266-71. 1993..These and other results strongly support the idea that Cln proteins function to activate Cdc28 at START... - Ste12 and Mcm1 regulate cell cycle-dependent transcription of FAR1L J Oehlen
Rockefeller University, New York, New York 10021, USA
Mol Cell Biol 16:2830-7. 1996..This indicates that transcription of FAR1 in the G1 phase is essential for accumulation of the protein and for pheromone-induced cell cycle arrest... - Accurate quantitation of protein expression and site-specific phosphorylationY Oda
The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
Proc Natl Acad Sci U S A 96:6591-6. 1999.... - Saccharomyces cerevisiae G1 cyclins differ in their intrinsic functional specificitiesK Levine
The Rockefeller University, New York, New York 10021, USA
Mol Cell Biol 16:6794-803. 1996..Consistent with the idea that Cln3 may be the primary transcriptional activator of CLN1, CLN2, and other genes, the activation of CLN2 transcription was found to be sensitive to the gene dosage of CLN3 but not to the gene dosage of CLN2... - Mechanisms controlling subcellular localization of the G(1) cyclins Cln2p and Cln3p in budding yeastM E Miller
The Rockefeller University, New York, New York 10021, USA
Mol Cell Biol 21:6292-311. 2001.... - Cln3-associated kinase activity in Saccharomyces cerevisiae is regulated by the mating factor pathwayD I Jeoung
The Rockefeller University, New York, New York 10021, USA
Mol Cell Biol 18:433-41. 1998.... - Structure-function analysis of the Saccharomyces cerevisiae G1 cyclin Cln2K N Huang
The Rockefeller University, New York, New York 10021, USA
Mol Cell Biol 17:4654-66. 1997..Some alleles demonstrated restricted patterns of defects, suggesting that these mutations may interfere with specific aspects of Cln2 function... - Isolation and characterization of new alleles of the cyclin-dependent kinase gene CDC28 with cyclin-specific functional and biochemical defectsK Levine
The Rockefeller University, New York, New York 10021, USA
Mol Cell Biol 18:290-302. 1998..Mutants of this sort should be useful in differentiating the function of Cdc28 complexed with different cyclin regulatory subunits... - Mutations in RAD27 define a potential link between G1 cyclins and DNA replicationE A Vallen
Rockefeller University, New York, New York 10021 6399, USA
Mol Cell Biol 15:4291-302. 1995.... - Over-expression of S. cerevisiae G1 cyclins restores the viability of alg1 N-glycosylation mutantsB K Benton
Rockefeller University, New York, NY 10021, USA
Curr Genet 29:106-13. 1996..Co-ordinate regulation of glycosylation, sugar nucleotide metabolism, and cell-cycle progression through G1 may be a feature that ensures adequate cell-wall precursors are present before bud emergence... - Retinoblastoma protein: combating algal bloomF R Cross
The Rockefeller University, 1230 York Ave, Box 237, New York, New York 10021, USA
Curr Biol 11:R824-7. 2001..The discovery of a homolog of the retinoblastoma protein (Rb) in a single-celled eukaryote--the alga Chlamydomonas--promises new and surprising insights into Rb's function in cell-cycle regulation... - 'Marker swap' plasmids: convenient tools for budding yeast molecular geneticsF R Cross
Rockefeller University, New York, NY 10021, USA
Yeast 13:647-53. 1997..These 'marker-swap' plasmids therefore eliminate the need for much subcloning to change markers. Marker-swapped alleles are acceptably stable mitotically and meiotically for most applications... - Forced periodic expression of G1 cyclins phase-locks the budding yeast cell cycleG Charvin
Laboratory of Yeast Molecular Genetics and Center for Studies in Physics and Biology, The Rockefeller University, New York, NY 10021, USA
Proc Natl Acad Sci U S A 106:6632-7. 2009..Our results provide quantitative insight into how cell size is integrated with the cell cycle oscillator... - Cyclin specificity: how many wheels do you need on a unicycle?M E Miller
The Rockefeller University, New York, NY 10021, USA
J Cell Sci 114:1811-20. 2001..Such targeting might occur through a combination of factors, including temporal expression, protein associations, and subcellular localization... - Cell cycle arrest caused by CLN gene deficiency in Saccharomyces cerevisiae resembles START-I arrest and is independent of the mating-pheromone signalling pathwayF R Cross
Rockefeller University, New York, New York 10021 6399
Mol Cell Biol 10:6482-90. 1990..These results are consistent with a specific CLN requirement for START transit... - Pheromone-dependent G1 cell cycle arrest requires Far1 phosphorylation, but may not involve inhibition of Cdc28-Cln2 kinase, in vivoA Gartner
Institute for Biochemistry and Molecular Cell Biology and Ludwig Boltzmann Forschungsstelle, University of Vienna, Vienna, Austria
Mol Cell Biol 18:3681-91. 1998..Surprisingly, Far1-associated Cdc28-Cln2 complexes are at best moderately inhibited in immunoprecipitation kinase assays, suggesting unconventional inhibitory mechanisms of Far1... - CLB5-dependent activation of late replication origins in S. cerevisiaeA D Donaldson
Department of Genetics, University of Washington, Seattle 98195 7360, USA
Mol Cell 2:173-82. 1998..In clb5 clb6 mutants, the other B-type cyclins (Clb1-4p) promote an S phase during which both early and late replication origins fire... - The pheromone receptors inhibit the pheromone response pathway in Saccharomyces cerevisiae by a process that is independent of their associated G alpha proteinJ P Hirsch
Department of Cell Biology and Anatomy, Mount Sinai Medical Center, New York, New York 10029
Genetics 135:943-53. 1993..The ability of the pheromone receptors to block the pheromone response signal in the absence of the G alpha subunit indicates that these receptors interact with another component of the signal transduction pathway... - Further characterization of a size control gene in Saccharomyces cerevisiaeF R Cross
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98104
J Cell Sci Suppl 12:117-27. 1989.... - Interaction between the MEC1-dependent DNA synthesis checkpoint and G1 cyclin function in Saccharomyces cerevisiaeE A Vallen
Department of Biology, Swarthmore College, Swarthmore, Pennsylvania 19081, USA
Genetics 151:459-71. 1999..Thus deletion of CLN1 and CLN2 could function similarly to overexpression of RNR1 in suppressing mec1 lethality... - DAF1, a mutant gene affecting size control, pheromone arrest, and cell cycle kinetics of Saccharomyces cerevisiaeF R Cross
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104
Mol Cell Biol 8:4675-84. 1988..5-fold; thus, DAF1-1 may be a hyperactive or deregulated allele of a nonessential gene involved in G1 size control. Multiple copies of DAF1-1 also greatly reduced the duration of the G1 phase of the cell cycle...