C Yan Cheng

Summary

Affiliation: The Rockefeller University
Country: USA

Publications

  1. ncbi request reprint AF-2364 [1-(2,4-dichlorobenzyl)-1H-indazole-3-carbohydrazide] is a potential male contraceptive: a review of recent data
    C Yan Cheng
    Population Council, Center for Biomedical Research, New York, NY 10021, USA
    Contraception 72:251-61. 2005
  2. pmc The biology of spermatogenesis: the past, present and future
    C Yan Cheng
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Philos Trans R Soc Lond B Biol Sci 365:1459-63. 2010
  3. pmc A peptide derived from laminin-γ3 reversibly impairs spermatogenesis in rats
    Linlin Su
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, 1230 York Avenue, New York, New York 10065, USA
    Nat Commun 3:1185. 2012
  4. pmc Rai14 (retinoic acid induced protein 14) is involved in regulating f-actin dynamics at the ectoplasmic specialization in the rat testis*
    Xiaojing Qian
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, New York, NY, USA
    PLoS ONE 8:e60656. 2013
  5. pmc A seamless trespass: germ cell migration across the seminiferous epithelium during spermatogenesis
    Claire Q F Wang
    Center for Biomedical Research, Population Council, New York, NY 10065, USA
    J Cell Biol 178:549-56. 2007
  6. pmc NC1 domain of collagen α3(IV) derived from the basement membrane regulates Sertoli cell blood-testis barrier dynamics
    Elissa W P Wong
    The Mary M Wohlford Laboratory for Male Contraceptive Research Center for Biomedical Research Population Council New York, NY USA
    Spermatogenesis 3:e25465. 2013
  7. pmc Regulation of drug transporters in the testis by environmental toxicant cadmium, steroids and cytokines
    Linlin Su
    The Mary M Wohlford Laboratory for Male Contraceptive Research Center for Biomedical Research Population Council New York, NY USA
    Spermatogenesis 2:285-293. 2012
  8. pmc Targeting testis-specific proteins to inhibit spermatogenesis: lesson from endocrine disrupting chemicals
    H T Wan
    Center for Biomedical Research, The Mary M Wohlford Laboratory for Male Contraceptive Research, Population Council, 1230 York Ave, New York, NY 10065, USA
    Expert Opin Ther Targets 17:839-55. 2013
  9. pmc Regulation of the blood-testis barrier by coxsackievirus and adenovirus receptor
    Linlin Su
    Center for Biomedical Research, Population Council, New York, New York 10065, USA
    Am J Physiol Cell Physiol 303:C843-53. 2012
  10. pmc Filamin A is a regulator of blood-testis barrier assembly during postnatal development in the rat testis
    Wenhui Su
    Population Council, New York, New York 10065, USA
    Endocrinology 153:5023-35. 2012

Research Grants

Collaborators

Detail Information

Publications127 found, 100 shown here

  1. ncbi request reprint AF-2364 [1-(2,4-dichlorobenzyl)-1H-indazole-3-carbohydrazide] is a potential male contraceptive: a review of recent data
    C Yan Cheng
    Population Council, Center for Biomedical Research, New York, NY 10021, USA
    Contraception 72:251-61. 2005
    ..These summaries are helpful to investigators in the field who seek to understand the molecular mechanism of action of AF-2364 in the rat testis and to explore its possible use for male contraception...
  2. pmc The biology of spermatogenesis: the past, present and future
    C Yan Cheng
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Philos Trans R Soc Lond B Biol Sci 365:1459-63. 2010
    ....
  3. pmc A peptide derived from laminin-γ3 reversibly impairs spermatogenesis in rats
    Linlin Su
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, 1230 York Avenue, New York, New York 10065, USA
    Nat Commun 3:1185. 2012
    ..The impairment of spermatogenesis suggests that this laminin peptide fragment may serve as a contraceptive in male rats...
  4. pmc Rai14 (retinoic acid induced protein 14) is involved in regulating f-actin dynamics at the ectoplasmic specialization in the rat testis*
    Xiaojing Qian
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, New York, NY, USA
    PLoS ONE 8:e60656. 2013
    ..In short, Rai14 is involved in the re-organization of actin filaments in Sertoli cells during the epithelial cycle, participating in conferring spermatid polarity and cell adhesion in the testis...
  5. pmc A seamless trespass: germ cell migration across the seminiferous epithelium during spermatogenesis
    Claire Q F Wang
    Center for Biomedical Research, Population Council, New York, NY 10065, USA
    J Cell Biol 178:549-56. 2007
    ..Knowledge on viral invasion and leukocyte transmigration has also shed insights into germ cell movement during spermatogenesis...
  6. pmc NC1 domain of collagen α3(IV) derived from the basement membrane regulates Sertoli cell blood-testis barrier dynamics
    Elissa W P Wong
    The Mary M Wohlford Laboratory for Male Contraceptive Research Center for Biomedical Research Population Council New York, NY USA
    Spermatogenesis 3:e25465. 2013
    ..These findings illustrate the presence of a local regulatory axis known as the BTB-basement membrane axis that regulates BTB restructuring during spermatogenesis...
  7. pmc Regulation of drug transporters in the testis by environmental toxicant cadmium, steroids and cytokines
    Linlin Su
    The Mary M Wohlford Laboratory for Male Contraceptive Research Center for Biomedical Research Population Council New York, NY USA
    Spermatogenesis 2:285-293. 2012
    ..Furthermore, cytokines and steroids were found to have differential effects in regulating the expression of drug transporters. Summary, the expression of drug transporters in the testis is regulated by toxicants, steroids and cytokines...
  8. pmc Targeting testis-specific proteins to inhibit spermatogenesis: lesson from endocrine disrupting chemicals
    H T Wan
    Center for Biomedical Research, The Mary M Wohlford Laboratory for Male Contraceptive Research, Population Council, 1230 York Ave, New York, NY 10065, USA
    Expert Opin Ther Targets 17:839-55. 2013
    ..Since many EDCs possess intrinsic estrogenic or androgenic activities, thus, the gonad is one of the major targets of EDCs...
  9. pmc Regulation of the blood-testis barrier by coxsackievirus and adenovirus receptor
    Linlin Su
    Center for Biomedical Research, Population Council, New York, New York 10065, USA
    Am J Physiol Cell Physiol 303:C843-53. 2012
    ..g., occludin) possibly mediated via its structural interactions with nonreceptor protein kinases, thereby modulating endocytic vesicle-mediated protein trafficking...
  10. pmc Filamin A is a regulator of blood-testis barrier assembly during postnatal development in the rat testis
    Wenhui Su
    Population Council, New York, New York 10065, USA
    Endocrinology 153:5023-35. 2012
    ..In short, filamin A participates in BTB assembly by regulating protein recruitment during postnatal development in the rat testis...
  11. ncbi request reprint Regulation of spermiogenesis, spermiation and blood-testis barrier dynamics: novel insights from studies on Eps8 and Arp3
    C Yan Cheng
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Biochem J 435:553-62. 2011
    ....
  12. pmc The blood-testis barrier and its implications for male contraception
    C Yan Cheng
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Pharmacol Rev 64:16-64. 2012
    ..Collectively, these findings illustrate multiple potential targets are present at the BTB for innovative contraceptive development and for better delivery of drugs to alleviate toxicant-induced reproductive dysfunction in men...
  13. pmc Polarity proteins and actin regulatory proteins are unlikely partners that regulate cell adhesion in the seminiferous epithelium during spermatogenesis
    C Y Cheng
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Population Council, New York, USA
    Histol Histopathol 26:1465-74. 2011
    ..Furthermore, these recent findings have unraveled some unexpected molecules to be targeted for male contraceptive development, which are also targets of toxicant-induced male reproductive dysfunction...
  14. doi request reprint New frontiers in nonhormonal male contraception
    C Yan Cheng
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Contraception 82:476-82. 2010
    ..In this review, we discuss several approaches for fertility control involving the testis that one day may lead to the development of a nonhormonal male contraceptive...
  15. pmc Ectoplasmic specialization: a friend or a foe of spermatogenesis?
    Helen H N Yan
    Center for Biomedical Research, Population Council, New York, NY 10021, USA
    Bioessays 29:36-48. 2007
    ..We discuss herein the mechanisms that maintain the cross-talk between ES and blood-testis barrier to facilitate cell movement and orientation in the seminiferous epithelium...
  16. pmc A local autocrine axis in the testes that regulates spermatogenesis
    C Yan Cheng
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Nat Rev Endocrinol 6:380-95. 2010
    ..This local regulatory axis is the emerging target for male contraception...
  17. pmc Regulation of blood-testis barrier dynamics by focal adhesion kinase (FAK): an unexpected turn of events
    C Yan Cheng
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, New York, NY, USA
    Cell Cycle 8:3493-9. 2009
    ..Lastly, we describe a working model, which can be used to design future functional experiments to explore the involvement of FAK in BTB dynamics by investigators in the field...
  18. pmc Regulation of spermatogenesis in the microenvironment of the seminiferous epithelium: new insights and advances
    C Yan Cheng
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Mol Cell Endocrinol 315:49-56. 2010
    ..These findings provide a new window of research for investigators in the field to tackle the functional regulation of spermatogenesis...
  19. ncbi request reprint Cell junction dynamics in the testis: Sertoli-germ cell interactions and male contraceptive development
    C Yan Cheng
    Population Council, Center for Biomedical Research, New York, New York 10021, USA
    Physiol Rev 82:825-74. 2002
    ..An in-depth survey in this subject has also identified several potential targets to be tackled to perturb spermatogenesis, which will likely lead to the development of novel male contraceptives...
  20. pmc An intracellular trafficking pathway in the seminiferous epithelium regulating spermatogenesis: a biochemical and molecular perspective
    C Yan Cheng
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, New York 10065, USA
    Crit Rev Biochem Mol Biol 44:245-63. 2009
    ..Herein, we provide a critical review based on the latest findings in the field regarding the regulation of these cellular events. These recent findings also open up a new window for investigators studying blood-tissue barriers...
  21. ncbi request reprint TGF-beta3 regulates anchoring junction dynamics in the seminiferous epithelium of the rat testis via the Ras/ERK signaling pathway: An in vivo study
    Weiliang Xia
    Population Council, Center for Biomedical Research, New York, NY 10021, USA
    Dev Biol 280:321-43. 2005
    ..Yet an agent that selectively disrupts AJs (e.g., AF-2364) can limit its effects exclusively at the Sertoli-germ cell adhesive site without perturbing the Sertoli-Sertoli TJs...
  22. ncbi request reprint Sertoli-germ cell anchoring junction dynamics in the testis are regulated by an interplay of lipid and protein kinases
    Michelle K Y Siu
    Center for Biomedical Research, Population Council, New York, New York 10021, USA
    J Biol Chem 280:25029-47. 2005
    ..In summary, these results illustrate that Sertoli-germ cell anchoring junction dynamics in the testis are regulated, at least in part, via the beta1-integrin/PI3K/PKB/ERK signaling pathway...
  23. ncbi request reprint Tumor necrosis factor {alpha} reversibly disrupts the blood-testis barrier and impairs Sertoli-germ cell adhesion in the seminiferous epithelium of adult rat testes
    Michelle W M Li
    Population Council, 1230 York Avenue, New York 10021, USA
    J Endocrinol 190:313-29. 2006
    ....
  24. ncbi request reprint Regulation of ectoplasmic specialization dynamics in the seminiferous epithelium by focal adhesion-associated proteins in testosterone-suppressed rat testes
    Ching Hang Wong
    Population Council, Center for Biomedical Research, New York, New York 10021, USA
    Endocrinology 146:1192-204. 2005
    ..In addition to its role in conferring cell adhesion formation, the p-FAK/c-Src protein complex apparently also regulates apical ES disruption via the ERK signaling pathway...
  25. ncbi request reprint Dynamin II interacts with the cadherin- and occludin-based protein complexes at the blood-testis barrier in adult rat testes
    Pearl P Y Lie
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, New York 10021, USA
    J Endocrinol 191:571-86. 2006
    ..Thus, this may permit the occludin/ZO-1 complexes to maintain the BTB integrity when the cadherin/catenin complexes are dissociated to facilitate germ cell movement...
  26. pmc P-glycoprotein regulates blood-testis barrier dynamics via its effects on the occludin/zonula occludens 1 (ZO-1) protein complex mediated by focal adhesion kinase (FAK)
    Linlin Su
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Population Council, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 108:19623-8. 2011
    ..These findings indicate that P-glycoprotein is involved in BTB restructuring during spermatogenesis but that P-glycoprotein-mediated restructuring does not "open up" the BTB to make it freely permeable to drugs...
  27. ncbi request reprint Regulation of Sertoli-germ cell adherens junction dynamics via changes in protein-protein interactions of the N-cadherin-beta-catenin protein complex which are possibly mediated by c-Src and myotubularin-related protein 2: an in vivo study using an androg
    Jiayi Zhang
    Population Council, Center for Biomedical Research, New York, New York 10021, USA
    Endocrinology 146:1268-84. 2005
    ..In summary, these results illustrate that the cadherin/catenin is a crucial cell adhesion complex that regulates AJ dynamics in the testis, and its functionality is likely modulated by the MTMR2/c-Src protein complex...
  28. pmc An autocrine axis in the testis that coordinates spermiation and blood-testis barrier restructuring during spermatogenesis
    Helen H N Yan
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 105:8950-5. 2008
    ....
  29. ncbi request reprint Regulation of Sertoli-germ cell adherens junction dynamics in the testis via the nitric oxide synthase (NOS)/cGMP/protein kinase G (PRKG)/beta-catenin (CATNB) signaling pathway: an in vitro and in vivo study
    Nikki P Y Lee
    Population Council, New York, New York 10021, USA
    Biol Reprod 73:458-71. 2005
    ..These results illustrate that the CDH2/CATNB-mediated adhesion function in the testis is regulated, at least in part, via the NOS/cGMP/PRKG/CATNB pathway...
  30. ncbi request reprint Disruption of Sertoli-germ cell adhesion function in the seminiferous epithelium of the rat testis can be limited to adherens junctions without affecting the blood-testis barrier integrity: an in vivo study using an androgen suppression model
    Weiliang Xia
    Population Council, New York, New York 10021, USA
    J Cell Physiol 205:141-57. 2005
    ....
  31. ncbi request reprint Regulation of blood-testis barrier dynamics: an in vivo study
    Ching Hang Wong
    Population Council, Center for Biomedical Research, 1230 York Avenue, New York, NY 10021, USA
    J Cell Sci 117:783-98. 2004
    ....
  32. ncbi request reprint Fer kinase/FerT and adherens junction dynamics in the testis: an in vitro and in vivo study
    Yong Mei Chen
    Population Council, New York, New York, 10021, USA
    Biol Reprod 69:656-72. 2003
    ..In summary, Fer kinase structurally associates with the N-cadherin/catenin protein complex in the testis and can possibly be used to mediate signaling function via the cadherin/catenin protein complex...
  33. ncbi request reprint C-Src and c-Yes are two unlikely partners of spermatogenesis and their roles in blood-testis barrier dynamics
    Xiang Xiao
    Center for Biomedical Research, Population Council, New York New York, USA
    Adv Exp Med Biol 763:295-317. 2012
    ..We also propose a hypothetical model on the mechanistic functions of c-Src and c-Yes in spermatogenesis so that functional experiments can be designed in future studies...
  34. ncbi request reprint Interactions among IQGAP1, Cdc42, and the cadherin/catenin protein complex regulate Sertoli-germ cell adherens junction dynamics in the testis
    Wing Yee Lui
    Population Council, Center for Biomedical Research, New York, New York 10021, USA
    J Cell Physiol 202:49-66. 2005
    ..These results illustrate that the interactions among IQGAP1, Cdc42, and beta-catenin are crucial to the regulation of Sertoli-germ cell, but not Sertoli-Sertoli cell, AJ dynamics in the seminiferous epithelium...
  35. pmc 14-3-3 Protein regulates cell adhesion in the seminiferous epithelium of rat testes
    Elissa W P Wong
    Center for Biomedical Research, Population Council, New York, New York 10065, USA
    Endocrinology 150:4713-23. 2009
    ..14-3-3theta may act as a molecular switch that coordinates these two cellular events in the seminiferous epithelium during spermatogenesis...
  36. ncbi request reprint Protein kinases and adherens junction dynamics in the seminiferous epithelium of the rat testis
    Nikki P Y Lee
    Population Council, 1230 York Avenue, New York, New York 10021, USA
    J Cell Physiol 202:344-60. 2005
    ..In short, the data reported herein have shown that c-Src, Csk, and CK2 are novel protein kinases in AJ dynamics in the testis...
  37. pmc Epidermal growth factor receptor pathway substrate 8 (Eps8) is a novel regulator of cell adhesion and the blood-testis barrier integrity in the seminiferous epithelium
    Pearl P Y Lie
    Center for Biomedical Research, Population Council, 1230 York Ave, New York, NY 10065, USA
    FASEB J 23:2555-67. 2009
    ....
  38. ncbi request reprint p-FAK-Tyr(397) regulates spermatid adhesion in the rat testis via its effects on F-actin organization at the ectoplasmic specialization
    Hin Ting Wan
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, New York, New York
    Am J Physiol Endocrinol Metab 305:E687-99. 2013
    ..These findings illustrate a mechanistic pathway mediated by p-FAK-Tyr(397) that regulates spermatid adhesion at the apical ES in vivo...
  39. pmc Targeted and reversible disruption of the blood-testis barrier by an FSH mutant-occludin peptide conjugate
    Ching Hang Wong
    Center for Biomedical Research, Population Council, 1230 York Ave, New York, NY 10021, USA
    FASEB J 21:438-48. 2007
    ..In summary, the use of an FSH mutant-occludin peptide conjugate is a feasible nanodevice to transiently compromise the BTB...
  40. pmc Focal adhesion kinase-Tyr407 and -Tyr397 exhibit antagonistic effects on blood-testis barrier dynamics in the rat
    Pearl P Y Lie
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 109:12562-7. 2012
    ....
  41. pmc Unraveling the molecular targets pertinent to junction restructuring events during spermatogenesis using the Adjudin-induced germ cell depletion model
    Weiliang Xia
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, New York 10021, USA
    J Endocrinol 192:563-83. 2007
    ....
  42. ncbi request reprint Is the cadherin/catenin complex a functional unit of cell-cell actin-based adherens junctions in the rat testis?
    Nikki P Y Lee
    Population Council, Center for Biomedical Research, New York, New York 10021, USA
    Biol Reprod 68:489-508. 2003
    ..These results thus unequivocally demonstrate that the cadherin/catenin complex, which can be up-regulated by testosterone, is indeed present between Sertoli and germ cells and is used for the assembly of functional AJs...
  43. pmc Perfluorooctanesulfonate (PFOS) perturbs male rat Sertoli cell blood-testis barrier function by affecting F-actin organization via p-FAK-Tyr(407): an in vitro study
    Hin Ting Wan
    The Mary M Wohlford Laboratory for Male Contraceptive Research H T W, D D M, C Y C, Center for Biomedical Research, Population Council, New York, New York 10065 and Department of Biology C K C W, Hong Kong Baptist University, Kowloon, Hong Kong, China
    Endocrinology 155:249-62. 2014
    ....
  44. ncbi request reprint Role of P-glycoprotein at the blood-testis barrier on adjudin distribution in the testis: a revisit of recent data
    Linlin Su
    Center for Biomedical Research, Population Council, New York, New York, USA
    Adv Exp Med Biol 763:318-33. 2012
    ..Herein, we summarize latest findings in this area of research, we also provide a critical evaluation on research areas that deserve attention in future studies...
  45. pmc Differential effects of testosterone and TGF-β3 on endocytic vesicle-mediated protein trafficking events at the blood-testis barrier
    Linlin Su
    Center for Biomedical Research, Population Council, New York, NY 10065, USA
    Exp Cell Res 316:2945-60. 2010
    ....
  46. pmc Regulation of blood-testis barrier dynamics by TGF-beta3 is a Cdc42-dependent protein trafficking event
    Elissa W P Wong
    Center for Biomedical Research, Population Council, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 107:11399-404. 2010
    ..In summary, Cdc42 is a crucial regulatory component in the TGF-beta3-mediated cascade of events that leads to the disruption of the TJ fibrils above the preleptotene spermatocytes to facilitate their transit...
  47. pmc Disruption of the blood-testis barrier integrity by bisphenol A in vitro: is this a suitable model for studying blood-testis barrier dynamics?
    Michelle W M Li
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Int J Biochem Cell Biol 41:2302-14. 2009
    ..In summary, bisphenol A reversibly perturbs the integrity of the blood-testis barrier in Sertoli cells in vitro, which can also serve as a suitable model for studying the dynamics of the blood-testis barrier...
  48. pmc Biology and regulation of ectoplasmic specialization, an atypical adherens junction type, in the testis
    Elissa W P Wong
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Biochim Biophys Acta 1778:692-708. 2008
    ..g., apical ES) in the testis. The biology and regulation based on recent studies in the testis are of interest to cell biologists in the field, in particular their regulation, which perhaps is applicable to tumorigenesis...
  49. ncbi request reprint Differential interactions between transforming growth factor-beta3/TbetaR1, TAB1, and CD2AP disrupt blood-testis barrier and Sertoli-germ cell adhesion
    Weiliang Xia
    Population Council, Center for Biomedical Research, 1230 York Avenue, New York, NY 10021, USA
    J Biol Chem 281:16799-813. 2006
    ..However, TGF-beta3 selectively disrupts Sertoli-germ cell adhesion in the seminiferous epithelium to facilitate germ cell migration without compromising BTB when TbetaRI interacts only with adaptor CD2AP...
  50. pmc Breast cancer resistance protein regulates apical ectoplasmic specialization dynamics stage specifically in the rat testis
    Xiaojing Qian
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, New York, NY 10065, USA
    Am J Physiol Endocrinol Metab 304:E757-69. 2013
    ..Indeed, a knockdown of Bcrp by RNAi in the testis perturbed the apical ES function, disrupting spermatid polarity and adhesion. In summary, Bcrp is a regulator of the F-actin-rich apical ES in the testis...
  51. ncbi request reprint TGF-beta3 regulates the blood-testis barrier dynamics via the p38 mitogen activated protein (MAP) kinase pathway: an in vivo study
    Wing Yee Lui
    Population Council, New York, New York 10021, USA
    Endocrinology 144:1139-42. 2003
    ..These results illustrate that BTB dynamics in vivo are regulated by the TGF-beta3/p38 MAP kinase pathway, which in turn determines the level of occludin at the site of Sertoli cells TJs...
  52. pmc Regulation of actin dynamics and protein trafficking during spermatogenesis--insights into a complex process
    Wenhui Su
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, New York, NY 10065, USA
    Crit Rev Biochem Mol Biol 48:153-72. 2013
    ..It is obvious that this model will be rapidly updated when more data are available in future years...
  53. pmc Secreted Frizzled-related protein 1 (sFRP1) regulates spermatid adhesion in the testis via dephosphorylation of focal adhesion kinase and the nectin-3 adhesion protein complex
    Elissa W P Wong
    Center for Biomedical Research, Population Council, Rockefeller University, New York, New York 10065, USA
    FASEB J 27:464-77. 2013
    ..In summary, this report highlights the critical role of sFRP1 in regulating spermiation via its effects on the FAK signaling and retention of nectin-3 adhesion complex at the apical ES...
  54. ncbi request reprint The apical ectoplasmic specialization-blood-testis barrier functional axis is a novel target for male contraception
    Ka Wai Mok
    Center for Biomedical Research, Population Council, New York, New York, USA
    Adv Exp Med Biol 763:334-55. 2012
    ....
  55. pmc c-Yes regulates cell adhesion at the blood-testis barrier and the apical ectoplasmic specialization in the seminiferous epithelium of rat testes
    Xiang Xiao
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Int J Biochem Cell Biol 43:651-65. 2011
    ..In summary, c-Yes regulates BTB and apical ES integrity by maintaining proper distribution of integral membrane proteins and actin filament organization at these sites...
  56. pmc Focal adhesion kinase is a blood-testis barrier regulator
    Erica R Siu
    Center for Biomedical Research, Population Council, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 106:9298-303. 2009
    ..Furthermore, FAK was shown to be a molecular target of cadmium because its knockdown would desensitize Sertoli cells to cadmium-induced TJ barrier disruption. In summary, FAK is a unique regulator of BTB dynamics in the testis...
  57. pmc Crosstalk between desmoglein-2/desmocollin-2/Src kinase and coxsackie and adenovirus receptor/ZO-1 protein complexes, regulates blood-testis barrier dynamics
    Pearl P Y Lie
    Population Council, Center for Biomedical Research, 1230 York Avenue, New York, NY 10065, United States
    Int J Biochem Cell Biol 42:975-86. 2010
    ..We hereby propose a novel regulatory protein complex composed of desmoglein-2, desmocollin-2, c-Src, coxsackie and adenovirus receptor and zonula occludens-1 at the blood-testis barrier...
  58. ncbi request reprint Cellular localization of sphingomyelin synthase 2 in the seminiferous epithelium of adult rat testes
    Nikki P Y Lee
    Population Council, Center for Biomedical Research, New York, NY 10021, USA
    J Endocrinol 192:17-32. 2007
    ..Collectively, these results suggest that SMS2 may play a crucial role in the lipid metabolism in acrosome formation and the plasma membrane restructuring from late round spermatids to early elongating spermatids...
  59. pmc Cytokines, polarity proteins, and endosomal protein trafficking and signaling-the sertoli cell blood-testis barrier system in vitro as a study model
    Xiang Xiao
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, New York, USA Department of Reproductive Physiology, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, China
    Methods Enzymol 534:181-94. 2014
    ..While we are using a Sertoli cell in vitro system that mimics the BTB in vivo, this approach should be applicable to virtually all mammalian cells. ..
  60. ncbi request reprint Zyxin, axin, and Wiskott-Aldrich syndrome protein are adaptors that link the cadherin/catenin protein complex to the cytoskeleton at adherens junctions in the seminiferous epithelium of the rat testis
    Nikki P Y Lee
    Population Council, New York, New York 10021, USA
    J Androl 25:200-15. 2004
    ..Collectively, these results illustrate that while these adaptors are structurally associated with the cadherin/catenin complex in the testis, they are regulated differently...
  61. pmc Palladin is a regulator of actin filament bundles at the ectoplasmic specialization in adult rat testes
    Xiaojing Qian
    Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, 1230 York Ave, New York, New York 10065, USA
    Endocrinology 154:1907-20. 2013
    ..Its knockdown in vivo also perturbed F-actin organization that led to a loss of spermatid polarity and adhesion, causing defects in spermatid transport and spermiation. In summary, palladin is an actin filament regulator at the ES...
  62. pmc Rictor/mTORC2 regulates blood-testis barrier dynamics via its effects on gap junction communications and actin filament network
    Ka Wai Mok
    Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, 1230 York Ave, New York, NY 10065, USA
    FASEB J 27:1137-52. 2013
    ..Rictor knockdown by RNAi was also found to impede Sertoli cell-cell GJ communication, disrupting protein distribution (e.g., occludin, ZO-1) at the BTB, illustrating that rictor is a crucial BTB regulator...
  63. pmc c-Yes regulates cell adhesion at the apical ectoplasmic specialization-blood-testis barrier axis via its effects on protein recruitment and distribution
    Xiang Xiao
    Center for Biomedical Research, Population Council, New York, NY 10065, USA
    Am J Physiol Endocrinol Metab 304:E145-59. 2013
    ..g., nectin-3) and possibly the result of changes in the underlying actin filaments at the BTB and the apical ES. These findings implicate that c-Yes is a likely target of male contraceptive research...
  64. ncbi request reprint Polarity protein complex Scribble/Lgl/Dlg and epithelial cell barriers
    Wen Hui Su
    Center for Biomedical Research, Population Council, New York New York, USA
    Adv Exp Med Biol 763:149-70. 2012
    ....
  65. pmc Intercellular adhesion molecule-2 is involved in apical ectoplasmic specialization dynamics during spermatogenesis in the rat
    Xiang Xiao
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    J Endocrinol 216:73-86. 2013
    ..Taken collectively, these results illustrate that ICAM2 plays an important role in apical ES dynamics during spermatogenesis...
  66. pmc The Scribble/Lgl/Dlg polarity protein complex is a regulator of blood-testis barrier dynamics and spermatid polarity during spermatogenesis
    Wenhui Su
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, New York, NY 10065, USA
    Endocrinology 153:6041-53. 2012
    ..In summary, the Scribble/Lgl/Dlg complex participates in the regulation of BTB dynamics and spermatid adhesion/polarity in the testis...
  67. pmc Coxsackie and adenovirus receptor (CAR) is a product of Sertoli and germ cells in rat testes which is localized at the Sertoli-Sertoli and Sertoli-germ cell interface
    Claire Q F Wang
    Center for Biomedical Research, The Population Council, 1230 York Avenue, New York, NY 10021, USA
    Exp Cell Res 313:1373-92. 2007
    ..It is possible that the receptor utilized by viral pathogens to breakthrough the epithelial barrier was also employed by developing germ cells to migrate through the inter-Sertoli cell junctions...
  68. pmc Connexin 43 and plakophilin-2 as a protein complex that regulates blood-testis barrier dynamics
    Michelle W M Li
    Center for Biomedical Research, Population Council, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 106:10213-8. 2009
    ....
  69. pmc Drug transporter, P-glycoprotein (MDR1), is an integrated component of the mammalian blood-testis barrier
    Linlin Su
    Population Council, Center for Biomedical Research, 1230 York Avenue, NY 10065, USA
    Int J Biochem Cell Biol 41:2578-87. 2009
    ..g., occludin, claudin-11 and JAM-A) was noted when testis lysates from Adjudin-treated rats were used for co-immunoprecipitation experiments, suggesting that P-gp may enhance BTB function during Sertoli-germ cell junction restructuring...
  70. pmc Par3/Par6 polarity complex coordinates apical ectoplasmic specialization and blood-testis barrier restructuring during spermatogenesis
    Elissa W P Wong
    Population Council, 1230 York Avenue, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 105:9657-62. 2008
    ....
  71. ncbi request reprint Sertoli-Sertoli and Sertoli-germ cell interactions and their significance in germ cell movement in the seminiferous epithelium during spermatogenesis
    Dolores D Mruk
    Population Council, Center for Biomedical Research, New York, New York 10021, USA
    Endocr Rev 25:747-806. 2004
    ..Other factors such as proteases, protease inhibitors, GTPases, kinases, and phosphatases also come into play. In this review, we focus on this cellular phenomenon, recapping recent developments in the field...
  72. ncbi request reprint Sertoli-germ cell adherens junction dynamics in the testis are regulated by RhoB GTPase via the ROCK/LIMK signaling pathway
    Wing Yee Lui
    Population Council, Center for Biomedical Research, New York, New York 10021, USA
    Biol Reprod 68:2189-206. 2003
    ..In summary, this report illustrates that Sertoli germ cell AJ dynamics are regulated, at least in part, via the integrin/ROCK/LIMK/cofilin signaling pathway...
  73. pmc rpS6 Regulates blood-testis barrier dynamics by affecting F-actin organization and protein recruitment
    Ka Wai Mok
    Center for Biomedical Research, Population Council, New York, New York 10065, USA
    Endocrinology 153:5036-48. 2012
    ..g. claudin-11 and occludin) to the BTB. Thus, rpS6 in the mTORC1 pathway regulates BTB restructuring via its effects on the F-actin organization and protein recruitment at the BTB...
  74. ncbi request reprint Adjudin, a potential male contraceptive, exerts its effects locally in the seminiferous epithelium of mammalian testes
    Ka Wai Mok
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, 1230 York Avenue, New York, New York 10065, USA
    Reproduction 141:571-80. 2011
    ..In this review, we highlight some of the recent advances and obstacles regarding the possible use of adjudin as a male contraceptive...
  75. pmc Blood-testis barrier dynamics are regulated by an engagement/disengagement mechanism between tight and adherens junctions via peripheral adaptors
    Helen H N Yan
    Population Council, 1230 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 102:11722-7. 2005
    ..Thus, this mechanism is used by the testis to facilitate AJ restructuring to accommodate germ cell migration while maintaining the BTB integrity...
  76. ncbi request reprint Dynamic cross-talk between cells and the extracellular matrix in the testis
    Michelle K Y Siu
    Population Council, Center for Biomedical Research, New York, New York 10021, USA
    Bioessays 26:978-92. 2004
    ....
  77. pmc Anchoring junctions as drug targets: role in contraceptive development
    Dolores D Mruk
    Population Council, Center for Biomedical Research, The Mary M Wohlford Laboratory for Male Contraceptive Research, 1230 York Avenue, New York, NY 10065, USA
    Pharmacol Rev 60:146-80. 2008
    ..Herein, we discuss a novel approach with the aim of developing a nonhormonal male contraceptive for future human use, one that involves perturbing adhesion between Sertoli and germ cells in the testis...
  78. pmc TGF-beta3 and TNFalpha perturb blood-testis barrier (BTB) dynamics by accelerating the clathrin-mediated endocytosis of integral membrane proteins: a new concept of BTB regulation during spermatogenesis
    Weiliang Xia
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Dev Biol 327:48-61. 2009
    ..In summary, this report demonstrates for the first time that BTB dynamics are regulated by TGF-beta3 and TNFalpha via an enhancement of protein endocytosis at the BTB...
  79. pmc Restricted Arp3 expression in the testis prevents blood-testis barrier disruption during junction restructuring at spermatogenesis
    Pearl P Y Lie
    Center for Biomedical Research, Population Council, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 107:11411-6. 2010
    ....
  80. ncbi request reprint Role of tissue inhibitor of metalloproteases-1 in junction dynamics in the testis
    Dolores D Mruk
    Population Council, Center for Biomedical Research, New York, New York 10021, USA
    J Androl 24:510-23. 2003
    ....
  81. ncbi request reprint Transforming growth factor beta3 regulates the dynamics of Sertoli cell tight junctions via the p38 mitogen-activated protein kinase pathway
    Wing Yee Lui
    Population Council, Center for Biomedical Research, New York, New York 10021, USA
    Biol Reprod 68:1597-612. 2003
    ..These results thus unequivocally demonstrate that TGFbeta3 utilizes the p38 MAP kinase pathway to regulate Sertoli cell TJ dynamics...
  82. ncbi request reprint Indazole carboxylic acids in male contraception
    C Yan Cheng
    The Population Council, Center for Biomedical Research, 1230 York Avenue, New York, NY, USA
    Contraception 65:265-8. 2002
    ..Neither compound affected the hypothalamus-pituitary-testicular axis, and both compounds were neither hepatotoxic nor nephrotoxic. These results suggest that these two compounds are safe for further development...
  83. ncbi request reprint TGF-betas: their role in testicular function and Sertoli cell tight junction dynamics
    Wing Yee Lui
    Population Council, Center for Biomedical Research, New York, NY 10021, USA
    Int J Androl 26:147-60. 2003
    ..This review summarizes recent advances of studies on TGF-betas in the testis, highlighting their regulatory role in TJ dynamics...
  84. ncbi request reprint Interactions of proteases, protease inhibitors, and the beta1 integrin/laminin gamma3 protein complex in the regulation of ectoplasmic specialization dynamics in the rat testis
    Michelle K Y Siu
    Population Council, Center for Biomedical Research, New York, New York 10021, USA
    Biol Reprod 70:945-64. 2004
    ..The net result of this interaction likely regulates germ cell movement in the seminiferous epithelium...
  85. pmc C-type natriuretic peptide regulates blood-testis barrier dynamics in adult rat testes
    Weiliang Xia
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10021
    Proc Natl Acad Sci U S A 104:3841-6. 2007
    ..In summary, CNP secreted by Sertoli and germ cells into the BTB microenvironment regulates BTB dynamics during spermatogenesis...
  86. ncbi request reprint Regulation of Sertoli cell tight junction dynamics in the rat testis via the nitric oxide synthase/soluble guanylate cyclase/3',5'-cyclic guanosine monophosphate/protein kinase G signaling pathway: an in vitro study
    Nikki P Y Lee
    Population Council, Center for Biomedical Research, 1230 York Avenue, New York, NY 10021, USA
    Endocrinology 144:3114-29. 2003
    ..Collectively, these results illustrate NOS is an important physiological regulator of TJ dynamics in the testis, exerting its effects via the NO/soluble guanylate cyclase/cGMP/protein kinase G signaling pathway...
  87. pmc Connexin 43 is critical to maintain the homeostasis of the blood-testis barrier via its effects on tight junction reassembly
    Michelle W M Li
    Center for Biomedical Research, Population Council, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 107:17998-8003. 2010
    ..In summary, Cx43 is crucial for TJ reassembly at the BTB during its cyclic restructuring throughout the seminiferous epithelial cycle of spermatogenesis...
  88. ncbi request reprint Ectoplasmic specialization, a testis-specific cell-cell actin-based adherens junction type: is this a potential target for male contraceptive development?
    Nikki P Y Lee
    Population Council, 1230 York Avenue, New York, NY 10021, USA
    Hum Reprod Update 10:349-69. 2004
    ..The potential to specifically target the ES may also mean that contraceptive action could be achieved without perturbing the hypothalamic-pituitary-testicular axis...
  89. pmc Cytokines and junction restructuring events during spermatogenesis in the testis: an emerging concept of regulation
    Michelle W M Li
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, United States
    Cytokine Growth Factor Rev 20:329-38. 2009
    ..Possible regulators that mediate cytokine-induced junction restructuring, including gap junction and extracellular matrix, and the role of testosterone on junction dynamics in the testis will also be discussed...
  90. pmc An occludin-focal adhesion kinase protein complex at the blood-testis barrier: a study using the cadmium model
    Erica R Siu
    Center for Biomedical Research, Population Council, 1230 York Avenue, New York, New York 10065, USA
    Endocrinology 150:3336-44. 2009
    ..Thus, these findings demonstrate that FAK is an integrated regulatory component of the occludin-ZO-1 protein complex, suggesting that functional studies can be performed to study the role of FAK in BTB dynamics...
  91. pmc Cytoskeletal dynamics and spermatogenesis
    Pearl P Y Lie
    The Mary M Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA
    Philos Trans R Soc Lond B Biol Sci 365:1581-92. 2010
    ....
  92. pmc Interleukin 1 alpha (IL1A) is a novel regulator of the blood-testis barrier in the rat
    Oli Sarkar
    Population Council, Center for Biomedical Research, New York, NY 10065, USA
    Biol Reprod 78:445-54. 2008
    ..It is possible that localized production of IL1A by Sertoli and/or germ cells in vivo results in BTB restructuring, and this may facilitate the movement of leptotene spermatocytes across the BTB...
  93. pmc Cross-talk between tight and anchoring junctions-lesson from the testis
    Helen H N Yan
    Center for Biomedical Research, The Population Council, New York, NY 10021, USA
    Adv Exp Med Biol 636:234-54. 2008
    ..Herein, we provide a critical review based on the recently available data regarding the cross-talk between TJ and anchoring junction to allow simultaneous maintenance of the BTB and germ cell movement across the seminiferous epithelium...
  94. ncbi request reprint Adhering junction dynamics in the testis are regulated by an interplay of beta 1-integrin and focal adhesion complex-associated proteins
    Michelle K Y Siu
    The Population Council, Center for Biomedical Research, New York, New York 10021, USA
    Endocrinology 144:2141-63. 2003
    ....
  95. pmc Coordinating cellular events during spermatogenesis: a biochemical model
    Pearl P Y Lie
    Center for Biomedical Research, New York, NY 10065, USA
    Trends Biochem Sci 34:366-73. 2009
    ..In this way, MAPKs would synchronize cell cycle progression in primary spermatocytes with junction remodeling and cell migration across the BTB...

Research Grants7

  1. The biology of blood-testis barrier dynamics
    C Yan Cheng; Fiscal Year: 2010
    ..It also offers new insight into: (i) developing novel approaches for male contraception, and (ii) the causes of unexplained male infertility. ..
  2. Junction Dynamics and Male Fertility Regulation
    C Yan Cheng; Fiscal Year: 2007
    ..In summary, these studies not only will define the regulation of AJ dynamics, they will identify new leads and innovative approaches for male contraceptive development. ..
  3. An in vivo model to study blood-testis barrier dynamics
    C Yan Cheng; Fiscal Year: 2007
    ..Second and perhaps most importantly, the disrupted BTB can be "resealed", making this model uniquely suitable to study the biology and regulation of BTB re-assembly during spermatogenesis. ..
  4. The biology of blood-testis barrier dynamics
    C Yan Cheng; Fiscal Year: 2009
    ..It also offers new insight into: (i) developing novel approaches for male contraception, and (ii) the causes of unexplained male infertility. ..
  5. The biology of blood-testis barrier dynamics
    C Yan Cheng; Fiscal Year: 2010
    ..It also offers new insight into: (i) developing novel approaches for male contraception, and (ii) the causes of unexplained male infertility. ..