Anchi Cheng

Summary

Affiliation: The Scripps Research Institute
Country: USA

Publications

  1. ncbi request reprint A graphical representation of image quality for three-dimensional structure analysis of two-dimensional crystals
    Anchi Cheng
    Department of Cell Biology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Acta Crystallogr A 60:351-4. 2004
  2. pmc A test-bed for optimizing high-resolution single particle reconstructions
    Scott M Stagg
    The National Resource for Automated Molecular Microscopy, Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Struct Biol 163:29-39. 2008
  3. doi request reprint Automated grid handling and image acquisition for two-dimensional crystal screening
    Anchi Cheng
    Department of Cell Biology, The National Resource for Automated Molecular Microscopy, The Scripps Research Institute, La Jolla, CA, USA
    Methods Mol Biol 955:297-305. 2013
  4. pmc Towards automated screening of two-dimensional crystals
    Anchi Cheng
    The National Resource for Automated Molecular Microscopy, Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, CB 129, La Jolla, CA 92037, USA
    J Struct Biol 160:324-31. 2007
  5. ncbi request reprint Does contamination buildup limit throughput for automated cryoEM?
    Anchi Cheng
    National Resource for Automated Molecular Microscopy and Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Struct Biol 154:303-11. 2006
  6. pmc Bootstrap resampling for voxel-wise variance analysis of three-dimensional density maps derived by image analysis of two-dimensional crystals
    Anchi Cheng
    The Scripps Research Institute, Department of Cell Biology, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Struct Biol 158:19-32. 2007
  7. pmc Appion: an integrated, database-driven pipeline to facilitate EM image processing
    Gabriel C Lander
    National Resource for Automated Molecular Microscopy, The Scripps Research Institute, CB 129, 10550 North Torrey Pines Rd, La Jolla, CA 92037, USA
    J Struct Biol 166:95-102. 2009
  8. pmc A toolbox for ab initio 3-D reconstructions in single-particle electron microscopy
    Neil R Voss
    National Resource for Automated Molecular Microscopy and Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Struct Biol 169:389-98. 2010
  9. pmc Initial evaluation of a direct detection device detector for single particle cryo-electron microscopy
    Anna Clare Milazzo
    Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA
    J Struct Biol 176:404-8. 2011
  10. ncbi request reprint Automated molecular microscopy: the new Leginon system
    Christian Suloway
    The Scripps Research Institute, La Jolla, CA 92037, USA
    J Struct Biol 151:41-60. 2005

Collaborators

Detail Information

Publications22

  1. ncbi request reprint A graphical representation of image quality for three-dimensional structure analysis of two-dimensional crystals
    Anchi Cheng
    Department of Cell Biology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Acta Crystallogr A 60:351-4. 2004
    ..The tilt geometry and image quality plot is especially useful for tracking the progress of data collection and for assessing the completeness of two data sets prior to determination of difference maps...
  2. pmc A test-bed for optimizing high-resolution single particle reconstructions
    Scott M Stagg
    The National Resource for Automated Molecular Microscopy, Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Struct Biol 163:29-39. 2008
    ..4A (FSC(0.5)) in which secondary structure is clearly discernable and the handedness of some of the alpha-helices in the GroEL structure can be determined...
  3. doi request reprint Automated grid handling and image acquisition for two-dimensional crystal screening
    Anchi Cheng
    Department of Cell Biology, The National Resource for Automated Molecular Microscopy, The Scripps Research Institute, La Jolla, CA, USA
    Methods Mol Biol 955:297-305. 2013
    ..A manual target selection bridges the two imaging passes. The two passes can be combined into one if objects of interests at different stages of trials can be reliably found using automated methods...
  4. pmc Towards automated screening of two-dimensional crystals
    Anchi Cheng
    The National Resource for Automated Molecular Microscopy, Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, CB 129, La Jolla, CA 92037, USA
    J Struct Biol 160:324-31. 2007
    ..While further development of these tools is certainly required for a turn-key system, the goal of fully automated screening appears to be within reach...
  5. ncbi request reprint Does contamination buildup limit throughput for automated cryoEM?
    Anchi Cheng
    National Resource for Automated Molecular Microscopy and Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Struct Biol 154:303-11. 2006
    ....
  6. pmc Bootstrap resampling for voxel-wise variance analysis of three-dimensional density maps derived by image analysis of two-dimensional crystals
    Anchi Cheng
    The Scripps Research Institute, Department of Cell Biology, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Struct Biol 158:19-32. 2007
    ....
  7. pmc Appion: an integrated, database-driven pipeline to facilitate EM image processing
    Gabriel C Lander
    National Resource for Automated Molecular Microscopy, The Scripps Research Institute, CB 129, 10550 North Torrey Pines Rd, La Jolla, CA 92037, USA
    J Struct Biol 166:95-102. 2009
    ..Presented here is a description of the design and architecture of the working Appion pipeline prototype and some results of its use...
  8. pmc A toolbox for ab initio 3-D reconstructions in single-particle electron microscopy
    Neil R Voss
    National Resource for Automated Molecular Microscopy and Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Struct Biol 169:389-98. 2010
    ....
  9. pmc Initial evaluation of a direct detection device detector for single particle cryo-electron microscopy
    Anna Clare Milazzo
    Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA
    J Struct Biol 176:404-8. 2011
    ..We also obtained a 3D reconstruction from ∼70K GroEL particles acquired using the DDD; the quality of the density map demonstrates the potential of this new recording device for cryoEM data acquisition...
  10. ncbi request reprint Automated molecular microscopy: the new Leginon system
    Christian Suloway
    The Scripps Research Institute, La Jolla, CA 92037, USA
    J Struct Biol 151:41-60. 2005
    ....
  11. doi request reprint Automation in single-particle electron microscopy connecting the pieces
    Dmitry Lyumkis
    National Resource for Automated Molecular Microscopy, Department of Cell Biology, The Scripps Research Institute, La Jolla, California, USA
    Methods Enzymol 483:291-338. 2010
    ..Rather, we aim to illustrate that automation is a comprehensive and versatile strategy that can deliver biological information on an unprecedented scale beyond the scope available with classical manual approaches...
  12. pmc Movies of ice-embedded particles enhance resolution in electron cryo-microscopy
    Melody G Campbell
    National Resource for Automated Molecular Microscopy, Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Structure 20:1823-8. 2012
    ..Using movies in this way is generally applicable to all cryo-EM samples and should also improve the performance of midrange electron microscopes that may have limited mechanical stability and beam coherence...
  13. ncbi request reprint Automated cryoEM data acquisition and analysis of 284742 particles of GroEL
    Scott M Stagg
    The National Resource for Automated Molecular Microscopy, Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Struct Biol 155:470-81. 2006
    ..In particular, we show that the resolution of the reconstructed 3D density maps improves with decreasing ice thickness. These data provide a basis for assessing the capabilities of high-throughput macromolecular microscopy...
  14. pmc Maximizing the potential of electron cryomicroscopy data collected using direct detectors
    David Veesler
    Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, United States National Resource for Automated Molecular Microscopy, The Scripps Research Institute, La Jolla, CA 92037, United States
    J Struct Biol 184:193-202. 2013
    ..We also compared the quality of this data to that collected with a FEI Titan Krios microscope equipped with a Falcon I direct detector, which provides a benchmark for data collected using a high-end electron microscope. ..
  15. doi request reprint Automation of data acquisition in electron crystallography
    Anchi Cheng
    Department of Cell Biology, The National Resource for Automated Molecular Microscopy, The Scripps Research Institute, La Jolla, CA, USA
    Methods Mol Biol 955:307-12. 2013
    ..General considerations for using automation software for acquiring high-resolution images of 2D crystals under low-dose conditions are presented. Protocol modifications specific to this application in Leginon are provided...
  16. ncbi request reprint Projection structure of full length connexin 43 by electron cryo-crystallography
    Anchi Cheng
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Cell Commun Adhes 10:187-91. 2003
    ..A difference map between the full length and trypsin-treated channels suggests that there are small but significant shifts in protein density upon removal of the C-tail...
  17. pmc Biophysical and ion channel functional characterization of the Torpedo californica nicotinic acetylcholine receptor in varying detergent-lipid environments
    Guillermo A Asmar-Rovira
    Departments of Cell Biology and Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Membr Biol 223:13-26. 2008
    ..Therefore, the nAChR can be stable and functional in lipid-analogue detergents or in detergents that retain moderate amounts of residual native lipids, but not in non-lipid-analogue detergents...
  18. ncbi request reprint Structure of full-length HIV-1 CA: a model for the mature capsid lattice
    Barbie K Ganser-Pornillos
    The Scripps Research Institute, Department of Cell Biology, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Cell 131:70-9. 2007
    ..Interactions between the two domains of CA further stabilize the hexamer and provide a structural explanation for the mechanism of action of known HIV-1 assembly inhibitors...
  19. ncbi request reprint The architecture of a water-selective pore in the lipid bilayer visualized by electron crystallography in vitreous ice
    Alok K Mitra
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Novartis Found Symp 245:33-46; discussion 46-50; 165-8. 2002
    ..Although not visible at the current resolution, the 3D structure suggests putative binding sites for water molecules in the size-selective pore...
  20. pmc On the freezing and identification of lipid monolayer 2-D arrays for cryoelectron microscopy
    Dianne W Taylor
    Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306 4380, USA
    J Struct Biol 160:305-12. 2007
    ..Though developed for microscopic examination of 2-D arrays, the method may have wider application to the preparation of single particle specimens for 3-D image reconstruction...
  21. pmc Milestones in electron crystallography
    Ludovic Renault
    Molecular and Cellular Biology, College of Biological Sciences, University of California at Davis, Briggs Hall, 1 Shields Ave, Davis, CA 95616, USA
    J Comput Aided Mol Des 20:519-27. 2006
    ..The server is also a repository for the 2dx software package, a user-friendly image processing system for 2D membrane protein crystals...
  22. doi request reprint Three-dimensional architecture of inorganic nanoarrays electrodeposited through a surface-layer protein mask
    Daniel B Allred
    Department of Chemical Engineering, University of Washington, Seattle, Washington 98195, USA
    Nano Lett 8:1434-8. 2008
    ....