Maria Elena Bottazzi

Summary

Affiliation: The George Washington University
Country: USA

Publications

  1. ncbi request reprint Progress in the development of a recombinant vaccine for human hookworm disease: the Human Hookworm Vaccine Initiative
    Peter J Hotez
    Department of Microbiology and Tropical Medicine, The George Washington University, Washington, DC, USA
    Int J Parasitol 33:1245-58. 2003
  2. pmc The role of cyclin D2 and p21/waf1 in human T-cell leukemia virus type 1 infected cells
    Kylene Kehn
    Department of Biochemistry and Molecular Biology, The George Washington University Medical Center, Washington, DC 20037, USA
    Retrovirology 1:6. 2004
  3. doi request reprint Model for product development of vaccines against neglected tropical diseases: a vaccine against human hookworm
    Maria Elena Bottazzi
    Department of Microbiology, Immunology and Tropical Medicine, The George Washington University, 2300 Eye Street NW, Ross Hall, Washington, DC 20037, USA
    Expert Rev Vaccines 7:1481-92. 2008
  4. ncbi request reprint An ounce of prevention on a budget: a nonprofit approach to developing vaccines against neglected diseases
    Maria Elena Bottazzi
    The George Washington University, The Human Hookworm Vaccine Initiative, Department of Microbiology, Immunology and Tropical Medicine, 2300 Eye Street NW, Ross Hall Room 733, Washington, DC 20037, USA
    Expert Rev Vaccines 5:189-98. 2006
  5. pmc Reduction of worm fecundity and canine host blood loss mediates protection against hookworm infection elicited by vaccination with recombinant Ac-16
    Ricardo T Fujiwara
    Department of Microbiology, Immunology and Tropical Medicine, The George Washington University, Ross Hall Room 727, 2300 Eye St NW, Washington, DC 20037, USA
    Clin Vaccine Immunol 14:281-7. 2007
  6. pmc Ancylostoma caninum MTP-1, an astacin-like metalloprotease secreted by infective hookworm larvae, is involved in tissue migration
    Angela L Williamson
    Department of Microbiology and Tropical Medicine, George Washington University Medical Center, Washington, DC, USA
    Infect Immun 74:961-7. 2006
  7. pmc Molecular cloning, biochemical characterization, and partial protective immunity of the heme-binding glutathione S-transferases from the human hookworm Necator americanus
    Bin Zhan
    Department of Microbiology and Tropical Medicine, The George Washington University and Sabin Vaccine Institute, Ross Hall 736, 2300 Eye St NW, Washington, DC 20037, USA
    Infect Immun 78:1552-63. 2010
  8. ncbi request reprint Immunogenicity of the hookworm Na-ASP-2 vaccine candidate: characterization of humoral and cellular responses after vaccination in the Sprague Dawley rat
    Ricardo T Fujiwara
    Department of Microbiology and Tropical Medicine, The George Washington University Medical Center, Washington, DC 20037, USA
    Hum Vaccin 1:123-8. 2005
  9. ncbi request reprint Vaccination with irradiated Ancylostoma caninum third stage larvae induces a Th2 protective response in dogs
    Ricardo T Fujiwara
    Department of Microbiology and Tropical Medicine, The George Washington University, 2300 Eye Street, Ross Hall Room 727, Medical Center, Washington, DC 20037, USA
    Vaccine 24:501-9. 2006
  10. doi request reprint Expression, purification, and molecular analysis of the Necator americanus glutathione S-transferase 1 (Na-GST-1): a production process developed for a lead candidate recombinant hookworm vaccine antigen
    Gaddam Narsa Goud
    Department of Microbiology, Immunology and Tropical Medicine, George Washington University Medical Center, Washington, DC, USA
    Protein Expr Purif 83:145-51. 2012

Collaborators

Detail Information

Publications26

  1. ncbi request reprint Progress in the development of a recombinant vaccine for human hookworm disease: the Human Hookworm Vaccine Initiative
    Peter J Hotez
    Department of Microbiology and Tropical Medicine, The George Washington University, Washington, DC, USA
    Int J Parasitol 33:1245-58. 2003
    ..Another challenge will be to produce anti-hookworm vaccine antigens at high yield low cost suitable for immunising large impoverished populations living in the developing nations of the tropics...
  2. pmc The role of cyclin D2 and p21/waf1 in human T-cell leukemia virus type 1 infected cells
    Kylene Kehn
    Department of Biochemistry and Molecular Biology, The George Washington University Medical Center, Washington, DC 20037, USA
    Retrovirology 1:6. 2004
    ..We have previously shown that Tax upregulates the cell cycle checkpoint proteins p21/waf1 and cyclin D2. Here we describe the consequences of upregulating these G1/S checkpoint regulators in HTLV-1 infected cells...
  3. doi request reprint Model for product development of vaccines against neglected tropical diseases: a vaccine against human hookworm
    Maria Elena Bottazzi
    Department of Microbiology, Immunology and Tropical Medicine, The George Washington University, 2300 Eye Street NW, Ross Hall, Washington, DC 20037, USA
    Expert Rev Vaccines 7:1481-92. 2008
    ....
  4. ncbi request reprint An ounce of prevention on a budget: a nonprofit approach to developing vaccines against neglected diseases
    Maria Elena Bottazzi
    The George Washington University, The Human Hookworm Vaccine Initiative, Department of Microbiology, Immunology and Tropical Medicine, 2300 Eye Street NW, Ross Hall Room 733, Washington, DC 20037, USA
    Expert Rev Vaccines 5:189-98. 2006
    ..Throughout this perspective, special emphasis is placed on the growing promise that product development public-private partnerships hold for developing vaccines for the world's poorest people...
  5. pmc Reduction of worm fecundity and canine host blood loss mediates protection against hookworm infection elicited by vaccination with recombinant Ac-16
    Ricardo T Fujiwara
    Department of Microbiology, Immunology and Tropical Medicine, The George Washington University, Ross Hall Room 727, 2300 Eye St NW, Washington, DC 20037, USA
    Clin Vaccine Immunol 14:281-7. 2007
    ..Our data support the use of Ac-16 as a potential candidate for vaccination against hookworm infection...
  6. pmc Ancylostoma caninum MTP-1, an astacin-like metalloprotease secreted by infective hookworm larvae, is involved in tissue migration
    Angela L Williamson
    Department of Microbiology and Tropical Medicine, George Washington University Medical Center, Washington, DC, USA
    Infect Immun 74:961-7. 2006
    ..The data strongly suggest that Ac-MTP-1 is critical for the invasion process of hookworm larvae, and moreover, that antibodies against the enzyme can neutralize its function and inhibit migration...
  7. pmc Molecular cloning, biochemical characterization, and partial protective immunity of the heme-binding glutathione S-transferases from the human hookworm Necator americanus
    Bin Zhan
    Department of Microbiology and Tropical Medicine, The George Washington University and Sabin Vaccine Institute, Ross Hall 736, 2300 Eye St NW, Washington, DC 20037, USA
    Infect Immun 78:1552-63. 2010
    ..In contrast, no protection was observed in vaccine trials with Na-GST-2 or Na-GST-3. On the basis of these and other preclinical data, Na-GST-1 is under possible consideration for further vaccine development...
  8. ncbi request reprint Immunogenicity of the hookworm Na-ASP-2 vaccine candidate: characterization of humoral and cellular responses after vaccination in the Sprague Dawley rat
    Ricardo T Fujiwara
    Department of Microbiology and Tropical Medicine, The George Washington University Medical Center, Washington, DC 20037, USA
    Hum Vaccin 1:123-8. 2005
    ..Host IL-6 levels were also significantly elevated. These data provide evidence that the immune response of the Na-ASP-2 vaccine is robust and durable and therefore suitable for the further clinical development of the Na-ASP-2 vaccine...
  9. ncbi request reprint Vaccination with irradiated Ancylostoma caninum third stage larvae induces a Th2 protective response in dogs
    Ricardo T Fujiwara
    Department of Microbiology and Tropical Medicine, The George Washington University, 2300 Eye Street, Ross Hall Room 727, Medical Center, Washington, DC 20037, USA
    Vaccine 24:501-9. 2006
    ....
  10. doi request reprint Expression, purification, and molecular analysis of the Necator americanus glutathione S-transferase 1 (Na-GST-1): a production process developed for a lead candidate recombinant hookworm vaccine antigen
    Gaddam Narsa Goud
    Department of Microbiology, Immunology and Tropical Medicine, George Washington University Medical Center, Washington, DC, USA
    Protein Expr Purif 83:145-51. 2012
    ..The expression and purification methods described here are suitable for further scale-up product development and for its use to design formulation processes suitable to generate a vaccine for clinical testing...
  11. ncbi request reprint Expression of the Necator americanus hookworm larval antigen Na-ASP-2 in Pichia pastoris and purification of the recombinant protein for use in human clinical trials
    Gaddam Narsa Goud
    Department of Microbiology and Tropical Medicine, The George Washington University Medical Center, Ross Hall 736, 2300 Eye Street NW, Washington, DC 20037, USA
    Vaccine 23:4754-64. 2005
    ..The processes developed and tested for the high yield production of recombinant Na-ASP-2 provide a foundation for clinical vaccine development...
  12. ncbi request reprint The impact of concurrent and treated Ancylostoma ceylanicum hookworm infections on the immunogenicity of a recombinant hookworm vaccine in hamsters
    Kashinath Ghosh
    Department of Microbiology and Tropical Medicine, George Washington University Medical Center, Washington, DC 20037, USA
    J Infect Dis 193:155-62. 2006
    ..The effect of concurrent (active) and treated hookworm infections on the immunogenicity of vaccination with the recombinant fusion protein Ay-Ancylostoma-secreted protein 2 was analyzed in the Golden Syrian hamster...
  13. ncbi request reprint New technologies for the control of human hookworm infection
    Peter J Hotez
    Department of Microbiology, Immunology, and Tropical Medicine, The George Washington University, 2300 I Street, NW Washington, DC 20037, USA
    Trends Parasitol 22:327-31. 2006
    ..g. tribendimidine) and a recombinant hookworm vaccine. It is hoped that, ultimately, anthelmintic vaccination will be linked to deworming as part of an expanded control package...
  14. ncbi request reprint Antibodies against a secreted protein from hookworm larvae reduce the intensity of hookworm infection in humans and vaccinated laboratory animals
    Jeffrey Bethony
    Department of Microbiology and Tropical Medicine, The George Washington University, Washington, DC 20037, USA
    FASEB J 19:1743-5. 2005
    ..These findings provide strong support for the development of an effective recombinant vaccine against hookworm infection in humans...
  15. doi request reprint Advances in vaccines against neglected tropical diseases: enhancing physical stability of a recombinant hookworm vaccine through biophysical and formulation studies
    Jordan L Plieskatt
    Department of Microbiology, Immunology and Tropical Medicine, The George Washington University, Washington, DC, USA
    Hum Vaccin Immunother 8:765-76. 2012
    ..Based on these evaluations, the recombinant Na-GST-1 antigen was reformulated and ultimately produced under Good Manufacturing Practices and with an acceptable stability profile...
  16. doi request reprint Ac-AP-12, a novel factor Xa anticoagulant peptide from the esophageal glands of adult Ancylostoma caninum
    Desheng Jiang
    Department of Microbiology, Immunology and Tropical Medicine, The George Washington University and the Sabin Vaccine Institute, Washington, DC 20037, USA
    Mol Biochem Parasitol 177:42-8. 2011
    ..Immunolocalization with specific rabbit antiserum showed that Ac-AP-12 was exclusively located in the esophageal glands of adult hookworm. Ac-AP-12 is hypothesized to facilitate both parasite blood feeding and digestion...
  17. ncbi request reprint Helminth infections of children: prospects for control
    Peter J Hotez
    Department of Microbiology and Tropical Medicine, The George Washington University Medical Center, Washington, DC 20037, USA
    Adv Exp Med Biol 568:135-44. 2005
  18. pmc Hookworm: "the great infection of mankind"
    Peter J Hotez
    Department of Microbiology and Tropical Medicine, The George Washington University, Washington, DC, USA
    PLoS Med 2:e67. 2005
  19. pmc The neglected tropical diseases of Latin America and the Caribbean: a review of disease burden and distribution and a roadmap for control and elimination
    Peter J Hotez
    Department of Microbiology, Immunology, and Tropical Medicine, The George Washington University and Sabin Vaccine Institute, Washington, DC, USA
    PLoS Negl Trop Dis 2:e300. 2008
    ..Ultimately, the roadmap for the control and elimination of the more widespread NTDs will require an inter-sectoral approach that bridges public health, social services, and environmental interventions...
  20. ncbi request reprint Hookworm infection
    Peter J Hotez
    Department of Microbiology and Tropical Medicine, George Washington University, Washington, DC 20037, USA
    N Engl J Med 351:799-807. 2004
  21. ncbi request reprint Mechanisms of HTLV-1 transformation
    Kylene Kehn
    Department of Biochemistry and Molecular Biology, School of Medicine and Health Sciences, The George Washington University, Washington, DC 20037, USA
    Front Biosci 9:2347-72. 2004
    ..Animal models of both Tax and the accessory proteins are also included based on the essential information on the transformation process in vivo that they provide...
  22. ncbi request reprint The HTLV-I Tax oncoprotein targets the retinoblastoma protein for proteasomal degradation
    Kylene Kehn
    Department of Biochemistry and Molecular Biology, The George Washington University School of Medicine, Washington, DC 20037, USA
    Oncogene 24:525-40. 2005
    ..Therefore, we propose a model whereby Tax targets Rb to the proteasome by acting as a molecular bridge bringing Rb into contact with the proteasome for degradation...
  23. ncbi request reprint Role of viral regulatory and accessory proteins in HIV-1 replication
    Anila Seelamgari
    Department of Biochemistry and Molecular Biology, School of Medicine and Health Sciences, The George Washington University, Washington, DC 20037, USA
    Front Biosci 9:2388-413. 2004
    ..In this review, we attempt to integrate information on the role of these regulatory and accessory proteins, emphasizing their interactions with other viral and cellular components, and the subsequent effect on viral replication...
  24. ncbi request reprint Protein profile of tax-associated complexes
    Kaili Wu
    Department of Biochemistry and Molecular Biology, School of Medicine and Health Sciences, The George Washington University, Washington, DC 20037, USA
    J Biol Chem 279:495-508. 2004
    ....
  25. ncbi request reprint In vitro cultured peripheral blood mononuclear cells from patients with chronic schistosomiasis mansoni show immunomodulation of cyclin D1,2,3 in the presence of soluble egg antigens
    Ana Carolina Campi-Azevedo
    Laboratorio de Imunologia Celular e Molecular, Instituto René Rachou Fiocruz, Fundacao Oswaldo Cruz, Avenida Augusto de Lima 1715, Barro Preto, 30190 002 Belo Horizonte, MG, Brazil
    Microbes Infect 9:1493-9. 2007
    ..The decreased expression of activation markers and frequency of cyclin D(1,2,3) in T cells may result in arrest of T cells in the G(0)/G(1) phase of the cell cycle, thus explaining the down-regulation observed in chronic schistosomiasis...
  26. pmc Vaccination with recombinant aspartic hemoglobinase reduces parasite load and blood loss after hookworm infection in dogs
    Alex Loukas
    Division of Infectious Diseases and Immunology, Queensland Institute of Medical Research, Brisbane, Queensland, Australia
    PLoS Med 2:e295. 2005
    ..The parasites subsequently digest hemoglobin in their intestines using a cascade of proteolysis that begins with the Ancylostoma caninum aspartic protease 1, APR-1...