Research Topics
Species | Maria Elena BottazziSummaryAffiliation: The George Washington University Country: USA Publications
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Detail Information
Publications
Progress in the development of a recombinant vaccine for human hookworm disease: the Human Hookworm Vaccine InitiativePeter J Hotez
Department of Microbiology and Tropical Medicine, The George Washington University, Washington, DC, USA
Int J Parasitol 33:1245-58. 2003..Another challenge will be to produce anti-hookworm vaccine antigens at high yield low cost suitable for immunising large impoverished populations living in the developing nations of the tropics...
The role of cyclin D2 and p21/waf1 in human T-cell leukemia virus type 1 infected cellsKylene Kehn
Department of Biochemistry and Molecular Biology, The George Washington University Medical Center, Washington, DC 20037, USA
Retrovirology 1:6. 2004..We have previously shown that Tax upregulates the cell cycle checkpoint proteins p21/waf1 and cyclin D2. Here we describe the consequences of upregulating these G1/S checkpoint regulators in HTLV-1 infected cells...
Model for product development of vaccines against neglected tropical diseases: a vaccine against human hookwormMaria Elena Bottazzi
Department of Microbiology, Immunology and Tropical Medicine, The George Washington University, 2300 Eye Street NW, Ross Hall, Washington, DC 20037, USA
Expert Rev Vaccines 7:1481-92. 2008....- An ounce of prevention on a budget: a nonprofit approach to developing vaccines against neglected diseasesMaria Elena Bottazzi
The George Washington University, The Human Hookworm Vaccine Initiative, Department of Microbiology, Immunology and Tropical Medicine, 2300 Eye Street NW, Ross Hall Room 733, Washington, DC 20037, USA
Expert Rev Vaccines 5:189-98. 2006..Throughout this perspective, special emphasis is placed on the growing promise that product development public-private partnerships hold for developing vaccines for the world's poorest people... - Reduction of worm fecundity and canine host blood loss mediates protection against hookworm infection elicited by vaccination with recombinant Ac-16Ricardo T Fujiwara
Department of Microbiology, Immunology and Tropical Medicine, The George Washington University, Ross Hall Room 727, 2300 Eye St NW, Washington, DC 20037, USA
Clin Vaccine Immunol 14:281-7. 2007..Our data support the use of Ac-16 as a potential candidate for vaccination against hookworm infection... - Ancylostoma caninum MTP-1, an astacin-like metalloprotease secreted by infective hookworm larvae, is involved in tissue migrationAngela L Williamson
Department of Microbiology and Tropical Medicine, George Washington University Medical Center, Washington, DC, USA
Infect Immun 74:961-7. 2006..The data strongly suggest that Ac-MTP-1 is critical for the invasion process of hookworm larvae, and moreover, that antibodies against the enzyme can neutralize its function and inhibit migration... - Molecular cloning, biochemical characterization, and partial protective immunity of the heme-binding glutathione S-transferases from the human hookworm Necator americanusBin Zhan
Department of Microbiology and Tropical Medicine, The George Washington University and Sabin Vaccine Institute, Ross Hall 736, 2300 Eye St NW, Washington, DC 20037, USA
Infect Immun 78:1552-63. 2010..In contrast, no protection was observed in vaccine trials with Na-GST-2 or Na-GST-3. On the basis of these and other preclinical data, Na-GST-1 is under possible consideration for further vaccine development... - Immunogenicity of the hookworm Na-ASP-2 vaccine candidate: characterization of humoral and cellular responses after vaccination in the Sprague Dawley ratRicardo T Fujiwara
Department of Microbiology and Tropical Medicine, The George Washington University Medical Center, Washington, DC 20037, USA
Hum Vaccin 1:123-8. 2005..Host IL-6 levels were also significantly elevated. These data provide evidence that the immune response of the Na-ASP-2 vaccine is robust and durable and therefore suitable for the further clinical development of the Na-ASP-2 vaccine... - Vaccination with irradiated Ancylostoma caninum third stage larvae induces a Th2 protective response in dogsRicardo T Fujiwara
Department of Microbiology and Tropical Medicine, The George Washington University, 2300 Eye Street, Ross Hall Room 727, Medical Center, Washington, DC 20037, USA
Vaccine 24:501-9. 2006.... - Expression, purification, and molecular analysis of the Necator americanus glutathione S-transferase 1 (Na-GST-1): a production process developed for a lead candidate recombinant hookworm vaccine antigenGaddam Narsa Goud
Department of Microbiology, Immunology and Tropical Medicine, George Washington University Medical Center, Washington, DC, USA
Protein Expr Purif 83:145-51. 2012..The expression and purification methods described here are suitable for further scale-up product development and for its use to design formulation processes suitable to generate a vaccine for clinical testing... - Expression of the Necator americanus hookworm larval antigen Na-ASP-2 in Pichia pastoris and purification of the recombinant protein for use in human clinical trialsGaddam Narsa Goud
Department of Microbiology and Tropical Medicine, The George Washington University Medical Center, Ross Hall 736, 2300 Eye Street NW, Washington, DC 20037, USA
Vaccine 23:4754-64. 2005..The processes developed and tested for the high yield production of recombinant Na-ASP-2 provide a foundation for clinical vaccine development... - The impact of concurrent and treated Ancylostoma ceylanicum hookworm infections on the immunogenicity of a recombinant hookworm vaccine in hamstersKashinath Ghosh
Department of Microbiology and Tropical Medicine, George Washington University Medical Center, Washington, DC 20037, USA
J Infect Dis 193:155-62. 2006..The hookworm-associated immunodepression may have important implications for design of clinical trials of human vaccines and vaccination strategies... - New technologies for the control of human hookworm infectionPeter J Hotez
Department of Microbiology, Immunology, and Tropical Medicine, The George Washington University, 2300 I Street, NW Washington, DC 20037, USA
Trends Parasitol 22:327-31. 2006..g. tribendimidine) and a recombinant hookworm vaccine. It is hoped that, ultimately, anthelmintic vaccination will be linked to deworming as part of an expanded control package... - Antibodies against a secreted protein from hookworm larvae reduce the intensity of hookworm infection in humans and vaccinated laboratory animalsJeffrey Bethony
Department of Microbiology and Tropical Medicine, The George Washington University, Washington, DC 20037, USA
FASEB J 19:1743-5. 2005..These findings provide strong support for the development of an effective recombinant vaccine against hookworm infection in humans... - Ac-AP-12, a novel factor Xa anticoagulant peptide from the esophageal glands of adult Ancylostoma caninumDesheng Jiang
Department of Microbiology, Immunology and Tropical Medicine, The George Washington University and the Sabin Vaccine Institute, Washington, DC 20037, USA
Mol Biochem Parasitol 177:42-8. 2011..Immunolocalization with specific rabbit antiserum showed that Ac-AP-12 was exclusively located in the esophageal glands of adult hookworm. Ac-AP-12 is hypothesized to facilitate both parasite blood feeding and digestion... - Helminth infections of children: prospects for controlPeter J Hotez
Department of Microbiology and Tropical Medicine, The George Washington University Medical Center, Washington, DC 20037, USA
Adv Exp Med Biol 568:135-44. 2005 - Hookworm: "the great infection of mankind"Peter J Hotez
Department of Microbiology and Tropical Medicine, The George Washington University, Washington, DC, USA
PLoS Med 2:e67. 2005 - The neglected tropical diseases of Latin America and the Caribbean: a review of disease burden and distribution and a roadmap for control and eliminationPeter J Hotez
Department of Microbiology, Immunology, and Tropical Medicine, The George Washington University and Sabin Vaccine Institute, Washington, DC, USA
PLoS Negl Trop Dis 2:e300. 2008..Ultimately, the roadmap for the control and elimination of the more widespread NTDs will require an inter-sectoral approach that bridges public health, social services, and environmental interventions... - Hookworm infectionPeter J Hotez
Department of Microbiology and Tropical Medicine, George Washington University, Washington, DC 20037, USA
N Engl J Med 351:799-807. 2004 - Advances in vaccines against neglected tropical diseases: enhancing physical stability of a recombinant hookworm vaccine through biophysical and formulation studiesJordan L Plieskatt
Department of Microbiology, Immunology and Tropical Medicine, The George Washington University, Washington, DC, USA
Hum Vaccin Immunother 8:765-76. 2012..Based on these evaluations, the recombinant Na-GST-1 antigen was reformulated and ultimately produced under Good Manufacturing Practices and with an acceptable stability profile... - Mechanisms of HTLV-1 transformationKylene Kehn
Department of Biochemistry and Molecular Biology, School of Medicine and Health Sciences, The George Washington University, Washington, DC 20037, USA
Front Biosci 9:2347-72. 2004..Animal models of both Tax and the accessory proteins are also included based on the essential information on the transformation process in vivo that they provide... - The HTLV-I Tax oncoprotein targets the retinoblastoma protein for proteasomal degradationKylene Kehn
Department of Biochemistry and Molecular Biology, The George Washington University School of Medicine, Washington, DC 20037, USA
Oncogene 24:525-40. 2005..Therefore, we propose a model whereby Tax targets Rb to the proteasome by acting as a molecular bridge bringing Rb into contact with the proteasome for degradation... - Role of viral regulatory and accessory proteins in HIV-1 replicationAnila Seelamgari
Department of Biochemistry and Molecular Biology, School of Medicine and Health Sciences, The George Washington University, Washington, DC 20037, USA
Front Biosci 9:2388-413. 2004..In this review, we attempt to integrate information on the role of these regulatory and accessory proteins, emphasizing their interactions with other viral and cellular components, and the subsequent effect on viral replication... - Protein profile of tax-associated complexesKaili Wu
Department of Biochemistry and Molecular Biology, School of Medicine and Health Sciences, The George Washington University, Washington, DC 20037, USA
J Biol Chem 279:495-508. 2004.... - In vitro cultured peripheral blood mononuclear cells from patients with chronic schistosomiasis mansoni show immunomodulation of cyclin D1,2,3 in the presence of soluble egg antigensAna Carolina Campi Azevedo
Laboratorio de Imunologia Celular e Molecular, Instituto René Rachou Fiocruz, Fundacao Oswaldo Cruz, Avenida Augusto de Lima 1715, Barro Preto, 30190 002 Belo Horizonte, MG, Brazil
Microbes Infect 9:1493-9. 2007..The decreased expression of activation markers and frequency of cyclin D(1,2,3) in T cells may result in arrest of T cells in the G(0)/G(1) phase of the cell cycle, thus explaining the down-regulation observed in chronic schistosomiasis... - Vaccination with recombinant aspartic hemoglobinase reduces parasite load and blood loss after hookworm infection in dogsAlex Loukas
Division of Infectious Diseases and Immunology, Queensland Institute of Medical Research, Brisbane, Queensland, Australia
PLoS Med 2:e295. 2005..The parasites subsequently digest hemoglobin in their intestines using a cascade of proteolysis that begins with the Ancylostoma caninum aspartic protease 1, APR-1...