Gary Bokoch

Summary

Affiliation: The Scripps Research Institute
Country: USA

Publications

  1. ncbi Control of neutrophil pseudopods by fluid shear: role of Rho family GTPases
    Ayako Makino
    Dept of Bioengineering, The Whitaker Institute of Biomedical Engineering, University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA
    Am J Physiol Cell Physiol 288:C863-71. 2005
  2. ncbi Phosphorylation of RhoGDI by p21-activated kinase 1
    Celine M DerMardirossian
    Department of Immunology and Cell Biology, The Scripps Research Institute, La Jolla, CA, USA
    Methods Enzymol 406:80-90. 2006
  3. ncbi Caspase-mediated activation of PAK2 during apoptosis: proteolytic kinase activation as a general mechanism of apoptotic signal transduction?
    G M Bokoch
    Departments of Immunology and Cell Biology IMM14, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Cell Death Differ 5:637-45. 1998
  4. ncbi Regulation of the phagocyte NADPH oxidase by Rac GTPase
    Gary M Bokoch
    Department of Immunology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, California 92037, USA
    Antioxid Redox Signal 8:1533-48. 2006
  5. ncbi Regulation of innate immunity by Rho GTPases
    Gary M Bokoch
    Department of Immunology and Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Trends Cell Biol 15:163-71. 2005
  6. pmc Emerging evidence for the importance of phosphorylation in the regulation of NADPH oxidases
    Gary M Bokoch
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California 92037, USA
    Antioxid Redox Signal 11:2429-41. 2009
  7. ncbi NADPH oxidases: not just for leukocytes anymore!
    Gary M Bokoch
    Department of Immunology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Trends Biochem Sci 28:502-8. 2003
  8. ncbi Current molecular models for NADPH oxidase regulation by Rac GTPase
    Gary M Bokoch
    Departments of Immunology and Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Blood 100:2692-6. 2002
  9. ncbi Biology of the p21-activated kinases
    Gary M Bokoch
    Departments of Immunology and Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Annu Rev Biochem 72:743-81. 2003
  10. ncbi Regulation of cell function by Rho family GTPases
    G M Bokoch
    Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Immunol Res 21:139-48. 2000

Collaborators

Detail Information

Publications69

  1. ncbi Control of neutrophil pseudopods by fluid shear: role of Rho family GTPases
    Ayako Makino
    Dept of Bioengineering, The Whitaker Institute of Biomedical Engineering, University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA
    Am J Physiol Cell Physiol 288:C863-71. 2005
    ..These results suggest that deactivation of Rac activity by fluid shear plays an important role in stable circulation of leukocytes...
  2. ncbi Phosphorylation of RhoGDI by p21-activated kinase 1
    Celine M DerMardirossian
    Department of Immunology and Cell Biology, The Scripps Research Institute, La Jolla, CA, USA
    Methods Enzymol 406:80-90. 2006
    ..We describe here methods for the analysis of RhoGDI phosphorylation and regulation by p21-activated kinase 1 (Pak1)...
  3. ncbi Caspase-mediated activation of PAK2 during apoptosis: proteolytic kinase activation as a general mechanism of apoptotic signal transduction?
    G M Bokoch
    Departments of Immunology and Cell Biology IMM14, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Cell Death Differ 5:637-45. 1998
    ..We hypothesize that this may reflect their important role in regulating the controlled and orderly demise of the dying cell...
  4. ncbi Regulation of the phagocyte NADPH oxidase by Rac GTPase
    Gary M Bokoch
    Department of Immunology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, California 92037, USA
    Antioxid Redox Signal 8:1533-48. 2006
    ....
  5. ncbi Regulation of innate immunity by Rho GTPases
    Gary M Bokoch
    Department of Immunology and Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Trends Cell Biol 15:163-71. 2005
    ..An understanding of the biochemical basis for such distinct activities should provide novel insights into the molecular details of Rho GTPase function and regulation in innate immunity...
  6. pmc Emerging evidence for the importance of phosphorylation in the regulation of NADPH oxidases
    Gary M Bokoch
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California 92037, USA
    Antioxid Redox Signal 11:2429-41. 2009
    ..We describe here the evidence for Nox regulation through the action of kinases, and speculate on how such regulatory mechanisms might contribute to the development of pathological disease states...
  7. ncbi NADPH oxidases: not just for leukocytes anymore!
    Gary M Bokoch
    Department of Immunology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Trends Biochem Sci 28:502-8. 2003
    ..Here, we describe our current understanding of Nox function, and discuss emerging paradigms for regulation of Nox activity by Rac GTPase and/or other cytosolic components...
  8. ncbi Current molecular models for NADPH oxidase regulation by Rac GTPase
    Gary M Bokoch
    Departments of Immunology and Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Blood 100:2692-6. 2002
    ..As a mechanistically detailed example of Rac GTPase signaling, the NADPH oxidase provides a potential paradigm for signaling by Rho family GTPases in general...
  9. ncbi Biology of the p21-activated kinases
    Gary M Bokoch
    Departments of Immunology and Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Annu Rev Biochem 72:743-81. 2003
    ..We propose here a key role for PAK action in coordinating the dynamics of the actin and microtubule cytoskeletons during directional motility of cells, as well as in other functions requiring cytoskeletal polarization...
  10. ncbi Regulation of cell function by Rho family GTPases
    G M Bokoch
    Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Immunol Res 21:139-48. 2000
    ..Areas of major interest include NADPH oxidase regulation by Rac2, Rac- and Cdc42-mediated control of the actin-myosin cytoskeleton via p21-activated kinase (PAK), and modulation of the apoptotic program by Rho GTPases and PAK...
  11. doi PAK'n it in: identification of a selective PAK inhibitor
    Gary M Bokoch
    Departments of Immunology and Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Chem Biol 15:305-6. 2008
    ..In this issue of Chemistry & Biology, Deacon et al. target allosteric transitions undergone during PAK activation to identify a selective inhibitor (Deacon et al., 2008)...
  12. pmc The molecular basis for adhesion-mediated suppression of reactive oxygen species generation by human neutrophils
    Tieming Zhao
    Department of Immunology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    J Clin Invest 112:1732-40. 2003
    ..These studies identify an integrin-mediated pathway in which Vav1 is as a strong candidate for the critical regulatory point in suppression of Rac2 activation and ROS generation during inflammatory responses...
  13. ncbi Phosphorylation of RhoGDI by Pak1 mediates dissociation of Rac GTPase
    Celine DerMardirossian
    Department of Immunology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Mol Cell 15:117-27. 2004
    ..These results support a role for Pak1-mediated RhoGDI phosphorylation as a mechanism for Cdc42-mediated Rac activation, and suggest the possibility of Rac-induced positive feed-forward regulation of Rac activity...
  14. pmc Reactive oxygen species regulate a slingshot-cofilin activation pathway
    Jun Sub Kim
    Department of Immunology and Microbial Science and Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Biol Cell 20:2650-60. 2009
    ..These results suggest that the formation of ROS by NADPH oxidases engages a SSH-1L-cofilin pathway to regulate cytoskeletal organization and cell migration...
  15. ncbi Antagonistic cross-talk between Rac and Cdc42 GTPases regulates generation of reactive oxygen species
    Becky A Diebold
    Departments of Immunology and Cell Biology, Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    J Biol Chem 279:28136-42. 2004
    ..We propose here a novel antagonism between Rac and Cdc42 GTPases at the level of the Nox proteins that modulates the generation of ROS used for host defense, cell signaling, and transformation...
  16. ncbi Regulation of Nox1 activity via protein kinase A-mediated phosphorylation of NoxA1 and 14-3-3 binding
    Jun Sub Kim
    Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 282:34787-800. 2007
    ....
  17. ncbi p21-activated kinase 1 phosphorylates and regulates 14-3-3 binding to GEF-H1, a microtubule-localized Rho exchange factor
    Frank T Zenke
    Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 279:18392-400. 2004
    ..Phosphorylation of GEF-H1 by PAK may be involved in regulation of GEF-H1 activity and may serve to coordinate Rho-, Rac-, and Cdc42-mediated signaling pathways...
  18. pmc GEF-H1 modulates localized RhoA activation during cytokinesis under the control of mitotic kinases
    Jörg Birkenfeld
    Departments of Immunology and Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Dev Cell 12:699-712. 2007
    ..Our results identify a GEF-H1-dependent mechanism to modulate localized RhoA activation during cytokinesis under the control of mitotic kinases...
  19. pmc Constitutive p21-activated kinase (PAK) activation in breast cancer cells as a result of mislocalization of PAK to focal adhesions
    Mary R Stofega
    Department of Immunology, The Scripps Research Institute, San Diego, California 92037, USA
    Mol Biol Cell 15:2965-77. 2004
    ..Abnormal regulation of PAK localization and activity may contribute to the tumorigenic properties of certain breast cancer cells...
  20. pmc Rac1 links leading edge and uropod events through Rho and myosin activation during chemotaxis
    Kersi N Pestonjamasp
    Departments of Immunology and Cell Biology IMM14, The Scripps Research Institute, La Jolla, CA, USA
    Blood 108:2814-20. 2006
    ..We conclude that Rac plays both positive and negative roles in the organization of the Rhomyosin "backness" program, thereby promoting stable polarity in chemotaxing neutrophils...
  21. ncbi Chronophin, a novel HAD-type serine protein phosphatase, regulates cofilin-dependent actin dynamics
    Antje Gohla
    The Scripps Research Institute, Departments of Immunology and Cell Biology, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Nat Cell Biol 7:21-9. 2005
    ..Our findings identify a physiological phospho-serine protein substrate for a mammalian HAD-type phosphatase and demonstrate that CIN is an important novel regulator of cofilin-mediated actin reorganization...
  22. pmc Structural basis of guanine nucleotide exchange mediated by the T-cell essential Vav1
    Jill E Chrencik
    Department of Cellular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Mol Biol 380:828-43. 2008
    ..Taken together, the data outline the detailed nature of Vav1's ability to contact a range of Rho GTPases using a novel protein-protein interaction network...
  23. ncbi Regulation of microtubule destabilizing activity of Op18/stathmin downstream of Rac1
    Torsten Wittmann
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 279:6196-203. 2004
    ..However, in vivo, Pak1 activity alone is not sufficient to phosphorylate Op18, indicating that additional pathways downstream of Rac1 are required for Op18 regulation...
  24. ncbi Signaling requirements for translocation of P-Rex1, a key Rac2 exchange factor involved in chemoattractant-stimulated human neutrophil function
    Tieming Zhao
    Department of Immunology, IMM14, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    J Leukoc Biol 81:1127-36. 2007
    ....
  25. pmc Phosphorylation of RhoGDI by Src regulates Rho GTPase binding and cytosol-membrane cycling
    Celine DerMardirossian
    Departments of Immunology and Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Biol Cell 17:4760-8. 2006
    ..These results suggest that Src-mediated RhoGDI phosphorylation is a novel physiological mechanism for regulating Rho GTPase cytosol membrane-cycling and activity...
  26. ncbi p21-activated kinase 1 (PAK1) interacts with the Grb2 adapter protein to couple to growth factor signaling
    Lorena A Puto
    Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 278:9388-93. 2003
    ..Blockade of this interaction decreased the epidermal growth factor-induced extension of membrane lamellae. Thus Grb2 may serve as an important mechanism for linking downstream PAK signaling to various upstream pathways...
  27. pmc Guanine nucleotide exchange factor-H1 regulates cell migration via localized activation of RhoA at the leading edge
    Perihan Nalbant
    Departments of Immunology and Microbial Science, and Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Biol Cell 20:4070-82. 2009
    ..This study establishes GEF-H1 as a critical organizer of key structural and signaling components of cell migration through the localized regulation of RhoA activity at the cell leading edge...
  28. ncbi Critical role of proline-rich tyrosine kinase 2 in reversion of the adhesion-mediated suppression of reactive oxygen species generation by human neutrophils
    Tieming Zhao
    Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Immunol 174:8049-55. 2005
    ..This activity constitutes a mechanism by which cytokines might lead to rapid elimination of invading pathogens by adherent neutrophils under normal conditions or enhance tissue damage in pathological states...
  29. pmc Regulation of leading edge microtubule and actin dynamics downstream of Rac1
    Torsten Wittmann
    Department of Cell Biology and Institute for Childhood and Neglected Diseases, La Jolla, CA 92037, USA
    J Cell Biol 161:845-51. 2003
    ..In addition, Rac1(Q61L) promoted lamellipodial actin polymerization and Pak-dependent retrograde flow. Together, these results indicate coordinated regulation of the two cytoskeletal systems in the leading edge of migrating cells...
  30. pmc Identification of a conserved Rac-binding site on NADPH oxidases supports a direct GTPase regulatory mechanism
    Yu Ya Kao
    Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 283:12736-46. 2008
    ..Our data identify a functional Rac interaction site conserved in Rac-dependent Noxs and support a direct regulatory interaction of Rac GTPases to promote activation of these NADPH oxidases...
  31. pmc Direct interaction between Tks proteins and the N-terminal proline-rich region (PRR) of NoxA1 mediates Nox1-dependent ROS generation
    Davide Gianni
    Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Eur J Cell Biol 90:164-71. 2011
    ..Taken together, these results clarify the molecular basis for the interaction between NoxA1 and the Tks proteins and may provide new insights into the pharmacological design of a more effective anti-metastatic strategy...
  32. ncbi Integrin-linked kinase regulates Bergmann glial differentiation during cerebellar development
    Richard Belvindrah
    The Scripps Research Institute, Department of Cell Biology, Institute for Childhood and Neglected Disease, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Mol Cell Neurosci 33:109-25. 2006
    ..We also show that granule cell precursor proliferation is affected in ILK-deficient mice, but our findings provide strong evidence that proliferative defects are a secondary consequence of ILK function in glia...
  33. ncbi Nucleotide exchange factor GEF-H1 mediates cross-talk between microtubules and the actin cytoskeleton
    Mira Krendel
    Department of Immunology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Nat Cell Biol 4:294-301. 2002
    ..Furthermore, these effects are inhibited by dominant-negative versions of GEF-H1. Thus, GEF-H1 links changes in microtubule integrity to Rho-dependent regulation of the actin cytoskeleton...
  34. ncbi Bacillus anthracis toxins inhibit human neutrophil NADPH oxidase activity
    Matthew A Crawford
    Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Immunol 176:7557-65. 2006
    ..Our results demonstrate that anthrax toxins effectively suppress human neutrophil-mediated innate immunity by inhibiting their ability to generate superoxide for bacterial killing...
  35. ncbi 14-3-3 regulates actin dynamics by stabilizing phosphorylated cofilin
    Antje Gohla
    Department of Immunology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Curr Biol 12:1704-10. 2002
    ..Taken together, these results suggest that 14-3-3zeta proteins may play a dynamic role in the regulation of cellular actin structures through the maintenance of phosphocofilin levels...
  36. pmc Anthrax edema toxin inhibits Nox1-mediated formation of reactive oxygen species by colon epithelial cells
    Jun Sub Kim
    Department of Immunology, Scripps Research Institute, La Jolla, Calif, USA
    J Innate Immun 1:145-52. 2009
    ..Inhibition of Nox1-mediated ROS formation in the gut epithelium may be a mechanism used by B. anthracis to circumvent the innate immune response...
  37. ncbi Affinity-based assay of Rho guanosine triphosphatase activation
    Mary Stofega
    Department of Immunology and Cell Biology, The Scripps Research Institute, La Jolla, CA, USA
    Methods Mol Biol 332:269-79. 2006
    ....
  38. ncbi GDIs: central regulatory molecules in Rho GTPase activation
    Celine DerMardirossian
    Departments of Immunology and Cell Biology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Trends Cell Biol 15:356-63. 2005
    ..We propose that phosphorylation mediated by diverse kinases might provide a means of controlling and coordinating Rho GTPase activation...
  39. ncbi Spatial and temporal analysis of Rac activation during live neutrophil chemotaxis
    Elisabeth M Gardiner
    Department of Immunology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Curr Biol 12:2029-34. 2002
    ..These data establish that Rac GTPase is spatially and temporally regulated to coordinate leading-edge extension and tail retraction during a complex motile response, the chemotaxis of human neutrophils...
  40. ncbi Rac and Rap GTPase activation assays
    Ulla G Knaus
    Department of Immunology, The Scripps Research Institute, La Jolla, CA, USA
    Methods Mol Biol 412:59-67. 2007
    ..Here, we describe affinity-based pull-down assays for detection of Rac/Cdc42 and Rap activity in stimulated neutrophils...
  41. pmc The involvement of the tyrosine kinase c-Src in the regulation of reactive oxygen species generation mediated by NADPH oxidase-1
    Davide Gianni
    Departments of Immunology and Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Biol Cell 19:2984-94. 2008
    ..Together, these results establish c-Src as an important regulator of Nox1 activity, and they may provide insight into the mechanisms of tumor formation in colon cancers...
  42. pmc Human neutrophils coordinate chemotaxis by differential activation of Rac1 and Rac2
    Hui Zhang
    Department of Immunology and Microbial Science, Scripps Research Institute, La Jolla, CA 92037 1092, USA
    J Immunol 183:2718-28. 2009
    ....
  43. pmc PyK2 and FAK connections to p190Rho guanine nucleotide exchange factor regulate RhoA activity, focal adhesion formation, and cell motility
    Yangmi Lim
    Department of Reproductive Medicine, Moores Cancer Center, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 180:187-203. 2008
    ..These studies elucidate a compensatory function for Pyk2 upon FAK loss and identify the FAK-p190RhoGEF complex as an important integrin-proximal regulator of FA formation during FN-stimulated cell motility...
  44. pmc Cofilin activity downstream of Pak1 regulates cell protrusion efficiency by organizing lamellipodium and lamella actin networks
    Violaine Delorme
    Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Dev Cell 13:646-62. 2007
    ..We propose that cofilin functions as a regulator of cell protrusion by modulating the spatial interaction of the lamellipodium and lamella in response to upstream signals...
  45. ncbi Mechanism of p21-activated kinase 6-mediated inhibition of androgen receptor signaling
    Nicolas Schrantz
    Department of Immunology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Biol Chem 279:1922-31. 2004
    ..Modulation of PAK6 activity may be responsible for regulation of AR signaling in various forms of prostate cancer...
  46. pmc Chronophin mediates an ATP-sensing mechanism for cofilin dephosphorylation and neuronal cofilin-actin rod formation
    Timothy Y Huang
    Departments of Immunology and Microbial Science, and of Cell Biology, The Scripps Research Institute, IMM14, 10550 N Torrey Pines Rd, La Jolla, CA 92122, USA
    Dev Cell 15:691-703. 2008
    ....
  47. pmc Novel p47(phox)-related organizers regulate localized NADPH oxidase 1 (Nox1) activity
    Davide Gianni
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA
    Sci Signal 2:ra54. 2009
    ..We propose that Tks organizers represent previously unrecognized members of an organizer superfamily that link Nox to localized ROS formation...
  48. pmc FGD2, a CDC42-specific exchange factor expressed by antigen-presenting cells, localizes to early endosomes and active membrane ruffles
    Christoph Huber
    Department of Immunology and Microbial Sciences, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 283:34002-12. 2008
    ..These findings are consistent with a role of FGD2 in leukocyte signaling and vesicle trafficking in cells specialized to present antigen in the immune system...
  49. pmc GEF-H1 couples nocodazole-induced microtubule disassembly to cell contractility via RhoA
    Yuan Chen Chang
    Departments of Immunology and Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Biol Cell 19:2147-53. 2008
    ..Conversely, RhoA activation and contractility are rescued by reintroduction of siRNA-resistant GEF-H1. Our studies reveal a critical role for a GEF-H1/RhoA/ROCK/MLC signaling pathway in mediating nocodazole-induced cell contractility...
  50. pmc Inactivation of rho GTPases by statins attenuates anthrax lethal toxin activity
    Aimee M DeCathelineau
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California 92037, USA
    Infect Immun 77:348-59. 2009
    ..The use of drugs capable of inhibiting Rho GTPase activity, such as statins, may provide a means to attenuate intoxication during B. anthracis infection...
  51. ncbi Transduction of proteins into intact neutrophils
    Tieming Zhao
    Department of Immunology, The Scripps Research Institute, La Jolla, CA, USA
    Methods Mol Biol 412:115-23. 2007
    ..This requires both molecular biology to generate the initial construct as well as special procedures for protein isolation and renaturation. However, it also results in highly effective functional protein delivery into human neutrophils...
  52. ncbi Cofilin phosphatases and regulation of actin dynamics
    Timothy Y Huang
    The Scripps Research Institute, Departments of Immunology and Cell Biology, 10550 N Torrey Pines Road, La Jolla, California 92037, USA
    Curr Opin Cell Biol 18:26-31. 2006
    ..These cofilin phosphatases are unrelated in sequence and regulatory properties, each potentially providing a unique mechanism for cofilin activation under varying biological circumstances...
  53. pmc Peptide inhibitors MAP the way towards fighting anthrax pathogenesis
    Aimee M DeCathelineau
    Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Biochem J 395:e1-3. 2006
    ....
  54. pmc Modulation of myosin light-chain phosphorylation by p21-activated kinase 1 in Escherichia coli invasion of human brain microvascular endothelial cells
    Rajyalakshmi S Rudrabhatla
    Division of Infectious Diseases, Childrens Hospital Los Angeles and Keck School of Medicine, University of Southern California, Los Angeles, California 90027, USA
    Infect Immun 71:2787-97. 2003
    ..coli invasion of HBMEC induces MLC phosphorylation by inhibiting the activity of PAK1 and the recruitment of phosphorylated MLC to the site of actin condensation beneath the bacteria for efficient internalization of E. coli into HBMEC...
  55. ncbi The integrin beta tail is required and sufficient to regulate adhesion signaling to Rac1
    Allison L Berrier
    The Center for Cell Biology and Cancer Research, Albany Medical College, Albany, NY 12208, USA
    J Cell Sci 115:4285-91. 2002
    ..Our findings indicate that integrin beta-tails are an important link between integrin engagement and Rac1 signaling, and that protein interactions initiated at beta tails are sufficient for integrins to regulate Rac1 activity...
  56. ncbi Phosphorylation of p85 beta PIX, a Rac/Cdc42-specific guanine nucleotide exchange factor, via the Ras/ERK/PAK2 pathway is required for basic fibroblast growth factor-induced neurite outgrowth
    Eun Young Shin
    Department of Biochemistry, College of Medicine, Chungbuk National University, Cheongju 361 763, Korea
    J Biol Chem 277:44417-30. 2002
    ..Our study defines a novel signaling pathway for bFGF-induced neurite outgrowth that involves activation of the PAK2-p85 betaPIX complex via the ERK cascade and subsequent translocation of this complex...
  57. pmc Epac/Rap and PKA are novel mechanisms of ANP-induced Rac-mediated pulmonary endothelial barrier protection
    Anna A Birukova
    Section of Pulmonary and Critical Care Medicine, Department of Medicine, University of Chicago, Chicago, Illinois 60637, USA
    J Cell Physiol 215:715-24. 2008
    ..These results suggest a novel mechanism of ANP protective effects against agonist-induced pulmonary EC barrier dysfunction via inhibition of Rho signaling by Epac/Rap1-Rac and PKA signaling cascades...
  58. ncbi Adhesion-related kinase repression of gonadotropin-releasing hormone gene expression requires Rac activation of the extracellular signal-regulated kinase pathway
    Melissa P Allen
    Department of Medicine, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    J Biol Chem 277:38133-40. 2002
    ..The data suggest that Ark suppresses GnRH gene expression via the coordinated activation of a Rac --> ERK signaling pathway and a distinct MEF2- dependent mechanism...
  59. ncbi Microbial killing: hold the bleach and pass the salt!
    Gary M Bokoch
    Nat Immunol 3:340-2. 2002
  60. ncbi Assay of Cdc42, Rac, and Rho GTPase activation by affinity methods
    Valerie Benard
    UPRESA 5018 CNRS, 31054 Toulouse, France
    Methods Enzymol 345:349-59. 2002
  61. pmc Facilitation of Ca(2+)-dependent exocytosis by Rac1-GTPase in bovine chromaffin cells
    Quanwen Li
    Department of Physiology, University of Michigan, Ann Arbor 48109, USA
    J Physiol 550:431-45. 2003
    ..In summary, Rac1 was activated by secretory stimuli and modulated the secretory pathway downstream of Ca2+ influx, partly through regulation of cytoskeletal organization...
  62. pmc Akt phosphorylation of serine 21 on Pak1 modulates Nck binding and cell migration
    Guo Lei Zhou
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Mol Cell Biol 23:8058-69. 2003
    ..This Tat-Nck peptide reduced Akt-stimulated cell migration. Together, these data suggest that Akt modulates the association of Pak with Nck to regulate cell migration...
  63. pmc Mechanism of human dermal fibroblast migration driven by type I collagen and platelet-derived growth factor-BB
    Wei Li
    The Division of Dermatology and the University of Southern California Norris Cancer Center, University of Southern California Keck School of Medicine, Los Angeles, California 90033, USA
    Mol Biol Cell 15:294-309. 2004
    ..The experiments demonstrate 1) the genes' individual roles and specificities, 2) their combined effects and sufficiency, and 3) the mechanisms of their intermolecular connections in HDF migration driven by collagen and PDGF-BB...
  64. ncbi ArgBP2gamma interacts with Akt and p21-activated kinase-1 and promotes cell survival
    Zeng Qiang Yuan
    Department of Pathology, University of South Florida College of Medicine and H Lee Moffitt Cancer Center, Tampa, Florida 33612, USA
    J Biol Chem 280:21483-90. 2005
    ..These data indicate that ArgBP2gamma is a physiological substrate of Akt, functions as an adaptor for Akt and PAK1, and plays a role in Akt/PAK1 cell survival pathway...
  65. ncbi GEF-H1 is involved in agonist-induced human pulmonary endothelial barrier dysfunction
    Anna A Birukova
    Sect of Pulmonary and Critical Care, Dept of Medicine, Biological Sciences Div, Univ of Chicago, 929 E 57th St, W410, Chicago, IL 60637, USA
    Am J Physiol Lung Cell Mol Physiol 290:L540-8. 2006
    ....
  66. ncbi Epoxycyclopentenone-containing oxidized phospholipids restore endothelial barrier function via Cdc42 and Rac
    Konstantin G Birukov
    Division of Pulmonary and Critical Care Medicine, Center for Translational Respiratory Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Circ Res 95:892-901. 2004
    ..Thus, oxidative modification of phospholipids during inflammation leads to the formation of novel regulators that may be critically involved in restoration of vascular barrier function...
  67. ncbi Beta-arrestin-dependent regulation of the cofilin pathway downstream of protease-activated receptor-2
    Maria Zoudilova
    Cell, Molecular, and Developmental Biology Program and Biomedical Sciences Division, University of California Riverside, B605 Statistics Road, Riverside, CA 92521, USA
    J Biol Chem 282:20634-46. 2007
    ..These findings identify cofilin as a novel target of beta-arrestin-dependent scaffolding and suggest that many PAR-2-induced processes may be independent of Galpha(q/11) protein coupling...
  68. doi Cellular functions of GEF-H1, a microtubule-regulated Rho-GEF: is altered GEF-H1 activity a crucial determinant of disease pathogenesis?
    Jörg Birkenfeld
    Direvo Biotech AG, Nattermannallee 1, D 50829 Cologne, Germany
    Trends Cell Biol 18:210-9. 2008
    ..We suggest that GEF-H1 could be a novel therapeutic target in multiple human diseases...
  69. ncbi Neutrophil methods and protocols. Preface
    Mark T Quinn
    Department of Veterinary Molecular Biology, Montana State University, Bozeman, USA
    Methods Mol Biol 412:vii-viii. 2007

Research Grants44

  1. REGULATION OF NEUTROPHIL RECEPTOR G PROTEIN INTERACTIONS
    Gary Bokoch; Fiscal Year: 2009
    ..Spatio-temporal evaluation of Rho activation will be related to Rac2 activity, as well as specific aspects of a) chemoattractant receptor signaling, b) motile behavior, and c) actin-myosin cytoskeletal dynamics. ..
  2. CONTROL OF PHAGOCYTE NADPH OXIDASE BY CYTOSOLIC PROTEINS
    Gary Bokoch; Fiscal Year: 2005
    ....
  3. Regulation of neutrophil receptor-G protein interactions
    Gary Bokoch; Fiscal Year: 2003
    ..The proposed studies should yield new insights into the regulation of cell motility and the inflammatory response of human leukocytes. ..
  4. G PROTEIN REGULATION OF THE NEUTROPHIL NADPH OXIDASE
    Gary Bokoch; Fiscal Year: 2004
    ..These studies will yield novel insights into the overall control of oxidant formation by phagocytic leukocytes and other cells. ..
  5. RHO GTPASES IN ADHESION/CHEMOATTRACTANT RECEPTOR CROSST
    Gary Bokoch; Fiscal Year: 2002
    ..Finally, transgenic mice lacking specific signaling proteins will be used to confirm the roles of these pathways in regulating components of the adhesion-regulated oxidative response. ..
  6. REGULATION OF NEUTROPHIL RECEPTOR G PROTEIN INTERACTIONS
    Gary Bokoch; Fiscal Year: 2006
    ..Spatio-temporal evaluation of Rho activation will be related to Rac2 activity as well as specific aspects of a) chemoattractant receptor signaling, b) motile behavior, and c) actin-myosin cytoskeletal dynamics. ..
  7. Cofilin/ADF Regulation in Rho GTPase Signaling
    Gary Bokoch; Fiscal Year: 2009
    ..Our studies will investigate a novel regulator of this important process. In addition, this protein is likely to play important roles in ischemic disorders and other stress responses. ..
  8. Cofilin / ADF Regulation in Rho GTPase Signaling
    Gary Bokoch; Fiscal Year: 2007
    ..The proposed studies shoutd give us unique information about the action of Rho GTPases to regulate CA function during cetl motility. ..
  9. Cofilin/ADF Regulation in Rho GTPase Signaling
    Gary Bokoch; Fiscal Year: 2007
    ..Our studies will investigate a novel regulator of this important process. In addition, this protein is likely to play important roles in ischemic disorders and other stress responses. ..
  10. G PROTEIN REGULATION OF THE NEUTROPHIL NADPH OXIDASE
    Gary Bokoch; Fiscal Year: 2007
    ..The biological roles of GDI kinases in modulating the GTPase-GDI regulatory cycle will be investigated. ..
  11. Cofilin / ADF Regulation in Rho GTPase Signaling
    Gary Bokoch; Fiscal Year: 2006
    ..The proposed studies should give us unique information about the action of Rho GTPases to regulate CA function during cell motility. ..
  12. RHO GTPASES IN ADHESION/CHEMOATTRACTANT RECEPTOR CROSST
    Gary Bokoch; Fiscal Year: 2001
    ..Finally, transgenic mice lacking specific signaling proteins will be used to confirm the roles of these pathways in regulating components of the adhesion-regulated oxidative response. ..
  13. REGULATION OF NEUTROPHIL RECEPTOR/G PROTEIN INTERACTIONS
    Gary Bokoch; Fiscal Year: 1999
    ..Using molecular and biochemical means, they will probe the mechanisms by which phosphatases control Pak activity, and will initiate studies on the relevance of such regulatory phosphatases to receptor-mediated Pak activation. ..
  14. G PROTEIN REGULATION OF THE NEUTROPHIL NADPH OXIDASE
    Gary Bokoch; Fiscal Year: 2000
    ....
  15. G PROTEIN REGULATION OF THE NEUTROPHIL NADPH OXIDASE
    Gary Bokoch; Fiscal Year: 1993
    ..It is anticipated that these studies might lead to novel therapeutic approaches to chronic inflammatory and rheumatic diseases...
  16. LOW MW G PROTEIN (RAP) IN NEUTROPHIL ACTIVATION
    Gary Bokoch; Fiscal Year: 1993
    ..The proposed studies will enable us to better understand the regulation of neutrophil activation, as well as to elucidate the normal cellular roles of rap and related G proteins...
  17. REGULATION OF NEUTROPHIL G PROTEIN INTERACTIONS
    Gary Bokoch; Fiscal Year: 1993
    ..In total, this grant describes an integrated approach to the elucidation of the molecular basis for signal transduction by chemoattractant receptors in human neutrophils...