Gunter Blobel

Summary

Affiliation: The Rockefeller University
Country: USA

Publications

  1. pmc Mlp-dependent anchorage and stabilization of a desumoylating enzyme is required to prevent clonal lethality
    Xiaolan Zhao
    Laboratory of Cell Biology, The Rockefeller University, New York, NY 10021, USA
    J Cell Biol 167:605-11. 2004
  2. pmc Structural and functional analysis of Nup133 domains reveals modular building blocks of the nuclear pore complex
    Ian C Berke
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA
    J Cell Biol 167:591-7. 2004
  3. doi request reprint Three-dimensional organization of chromatids by nuclear envelope-associated structures
    Gunter Blobel
    Laboratory of Cell Biology, The Rockefeller University Howard Hughes Medical Institute, New York, New York 10065, USA
    Cold Spring Harb Symp Quant Biol 75:545-54. 2010
  4. pmc Structure of a trimeric nucleoporin complex reveals alternate oligomerization states
    Vivien Nagy
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 106:17693-8. 2009
  5. pmc Structural and functional analysis of Nup120 suggests ring formation of the Nup84 complex
    Hyuk Soo Seo
    Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 106:14281-6. 2009
  6. ncbi request reprint Structural basis for the function of the beta subunit of the eukaryotic signal recognition particle receptor
    Thomas Schwartz
    Howard Hughes Medical Institute, Laboratory of Cell Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Cell 112:793-803. 2003
  7. doi request reprint A fence-like coat for the nuclear pore membrane
    Erik W Debler
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Mol Cell 32:815-26. 2008
  8. pmc Structural and functional analysis of the interaction between the nucleoporin Nup98 and the mRNA export factor Rae1
    Yi Ren
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 107:10406-11. 2010
  9. pmc Cohesin subunit SMC1 associates with mitotic microtubules at the spindle pole
    Richard W Wong
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 105:15441-5. 2008
  10. pmc Architecture of a coat for the nuclear pore membrane
    Kuo Chiang Hsia
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Cell 131:1313-26. 2007

Collaborators

Detail Information

Publications37

  1. pmc Mlp-dependent anchorage and stabilization of a desumoylating enzyme is required to prevent clonal lethality
    Xiaolan Zhao
    Laboratory of Cell Biology, The Rockefeller University, New York, NY 10021, USA
    J Cell Biol 167:605-11. 2004
    ..Together, our results reveal that Mlps play important roles in regulating Ulp1 and subsequently affect sumoylation stasis, growth, and DNA repair...
  2. pmc Structural and functional analysis of Nup133 domains reveals modular building blocks of the nuclear pore complex
    Ian C Berke
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA
    J Cell Biol 167:591-7. 2004
    ..These and several other repeat-based motifs appear to be major elements of nucleoporins, indicating a level of structural repetition that may conceptually simplify the assembly and disassembly of this huge protein complex...
  3. doi request reprint Three-dimensional organization of chromatids by nuclear envelope-associated structures
    Gunter Blobel
    Laboratory of Cell Biology, The Rockefeller University Howard Hughes Medical Institute, New York, New York 10065, USA
    Cold Spring Harb Symp Quant Biol 75:545-54. 2010
    ....
  4. pmc Structure of a trimeric nucleoporin complex reveals alternate oligomerization states
    Vivien Nagy
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 106:17693-8. 2009
    ..Docking the crystal structures of all heptamer components into the EM envelope constitutes a major advance toward the completion of the structural characterization of the Nup84 complex...
  5. pmc Structural and functional analysis of Nup120 suggests ring formation of the Nup84 complex
    Hyuk Soo Seo
    Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 106:14281-6. 2009
    ..These results illuminate important roles of terminal unstructured segments in nucleoporins for the architecture, function, and assembly of the NPC...
  6. ncbi request reprint Structural basis for the function of the beta subunit of the eukaryotic signal recognition particle receptor
    Thomas Schwartz
    Howard Hughes Medical Institute, Laboratory of Cell Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Cell 112:793-803. 2003
    ..The results suggest that the GTP/GDP switch cycle of SR beta functions as a regulatory switch for the receptor dimerization. We discuss the implications for the translocation pathway...
  7. doi request reprint A fence-like coat for the nuclear pore membrane
    Erik W Debler
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Mol Cell 32:815-26. 2008
    ..Shared architectural and mechanistic principles with the COPII coat indicate a common evolutionary origin and support the notion that the NPC coat represents another class of membrane coats...
  8. pmc Structural and functional analysis of the interaction between the nucleoporin Nup98 and the mRNA export factor Rae1
    Yi Ren
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 107:10406-11. 2010
    ..Together, these data suggest that the Rae1*Nup98 complex directly binds to the mRNP at several stages of the mRNA export pathway...
  9. pmc Cohesin subunit SMC1 associates with mitotic microtubules at the spindle pole
    Richard W Wong
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 105:15441-5. 2008
    ..Imbalances in these assembly pathways caused formation of multipolar spindles. Our data suggest that cohesin's known bundling function for chromatids in mitotic and interphase cells extends to microtubules at the spindle pole...
  10. pmc Architecture of a coat for the nuclear pore membrane
    Kuo Chiang Hsia
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Cell 131:1313-26. 2007
    ..Our model proposes that the hetero-octamer would vertically traverse and connect the four stacked rings...
  11. pmc Cell-cycle-dependent phosphorylation of the nuclear pore Nup107-160 subcomplex
    Joseph S Glavy
    Laboratories of Mass Spectrometry and Gaseous Ion Chemistry, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 104:3811-6. 2007
    ....
  12. ncbi request reprint Carrier-independent nuclear import of the transcription factor PU.1 via RanGTP-stimulated binding to Nup153
    Hualin Zhong
    Laboratory of Cell Biology, Howard Hughes Medical Institute, New York, New York 10031, USA
    J Biol Chem 280:10675-82. 2005
    ..1 toward the nuclear side of the nuclear pore complex by increasing its affinity for Nup153. This notion was confirmed by ultrastructural studies using gold-labeled PU.1 in permeabilized cells...
  13. pmc Structural and functional analysis of the interaction between the nucleoporin Nup214 and the DEAD-box helicase Ddx19
    Johanna Napetschnig
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 106:3089-94. 2009
    ..Repeat of these cycles would remove proteins from a mRNP, one at a time, akin to a ratchet mechanism for mRNA export...
  14. pmc A SUMO ligase is part of a nuclear multiprotein complex that affects DNA repair and chromosomal organization
    Xiaolan Zhao
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 102:4777-82. 2005
    ..We propose that Mms21 sumoylates proteins involved in these diverse processes and that the other members of the complex, particularly Smc5/6, facilitate proper substrate sumoylation by localizing Mms21 to specific chromosomal regions...
  15. ncbi request reprint Karyopherin-mediated import of integral inner nuclear membrane proteins
    Megan C King
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA
    Nature 442:1003-7. 2006
    ..We also provide evidence that specific nuclear pore complex proteins contribute to this process, suggesting a role for signal-mediated alterations in the nuclear pore complex to allow for passage of INM proteins along the pore membrane...
  16. pmc Structural and functional analysis of an essential nucleoporin heterotrimer on the cytoplasmic face of the nuclear pore complex
    Kimihisa Yoshida
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 108:16571-6. 2011
    ..Notably, each of the three nucleoporins contains additional nuclear pore complex binding sites, distinct from those that were defined here in the heterotrimeric Nup82•Nup159•Nup116 complex...
  17. pmc Depletion of a single nucleoporin, Nup107, prevents the assembly of a subset of nucleoporins into the nuclear pore complex
    Thomas Boehmer
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 100:981-5. 2003
    ..These data indicate redundancy of Nups in the function of the mammalian NPC...
  18. pmc Structural evolution of the membrane-coating module of the nuclear pore complex
    Xiaoping Liu
    Laboratory of Cell Biology, Rockefeller University, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 109:16498-503. 2012
    ..Another contact region is situated toward the end of the helical region of Sp-Nup120(1-950). Our findings provide information about the evolution and the assembly of the coatomer module of the nuclear pore complex...
  19. ncbi request reprint Highway to the inner nuclear membrane: rules for the road
    C Patrick Lusk
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Nat Rev Mol Cell Biol 8:414-20. 2007
    ..Here we develop a set of rules that might govern the transport of proteins to the inner nuclear membrane...
  20. pmc Three-dimensional structure and flexibility of a membrane-coating module of the nuclear pore complex
    Martin Kampmann
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, New York, USA
    Nat Struct Mol Biol 16:782-8. 2009
    ..Notably, the same organizational principle is found in the clathrin triskelion, which may share a common evolutionary origin with the heptameric complex...
  21. ncbi request reprint Uncoupling Kapbeta2 substrate dissociation and ran binding
    Yuh Min Chook
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA
    Biochemistry 41:6955-66. 2002
    ..These results support our previously proposed GTPase-mediated Kapbeta2-substrate dissociation mechanism where the acidic loop of Kapbeta2 physically couples distinct Ran and substrate binding sites...
  22. ncbi request reprint Structure of Nup58/45 suggests flexible nuclear pore diameter by intermolecular sliding
    Ivo Melcák
    Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 315:1729-32. 2007
    ..We propose that circumferential sliding plays a role in adjusting the diameter of the central transport channel...
  23. pmc Ring cycle for dilating and constricting the nuclear pore
    Sozanne R Solmaz
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 110:5858-63. 2013
    ....
  24. ncbi request reprint Crystal structure of the N-terminal domain of Nup358/RanBP2
    Susanne A Kassube
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    J Mol Biol 423:752-65. 2012
    ..Together, these data suggest that the NTD contributes to mRNP remodeling events at the cytoplasmic face of the NPC...
  25. pmc Molecular basis for the autoregulation of the protein acetyl transferase Rtt109
    Pete Stavropoulos
    Laboratory of Cell Biology, Rockefeller University and Howard Hughes Medical Institute, 1230 York Avenue, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 105:12236-41. 2008
    ..We propose a model in which the autoacetylation of Rtt109 is crucial for the regulation of its catalytic activity...
  26. pmc Crystal structure of the N-terminal domain of the human protooncogene Nup214/CAN
    Johanna Napetschnig
    Laboratory of Cell Biology and Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 104:1783-8. 2007
    ..We propose a mechanism by which the C-terminal peptide extension is involved in NPC assembly...
  27. pmc Structure and nucleic acid binding activity of the nucleoporin Nup157
    Hyuk Soo Seo
    Laboratory of Cell Biology and Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10065
    Proc Natl Acad Sci U S A 110:16450-5. 2013
    ..The predicted four hinge regions indicate an intrinsic flexibility of Nup157, which could contribute to structural plasticity within the NPC...
  28. pmc Circular permutation as a tool to reduce surface entropy triggers crystallization of the signal recognition particle receptor beta subunit
    Thomas U Schwartz
    Howard Hughes Medical Institute, Laboratory of Cell Biology, The Rockefeller University, New York, New York 10021, USA
    Protein Sci 13:2814-8. 2004
    ..The success of this method profits from prior knowledge of the domain fold, which is becoming increasingly common in today's postgenomic era...
  29. pmc Molecular architecture of the transport channel of the nuclear pore complex
    Sozanne R Solmaz
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Cell 147:590-602. 2011
    ..3 MDa and contributing 256 phenylalanine-glycine repeat regions. We propose that the occupancy of these repeat regions with transport receptors modulates ring diameter and transport activity...
  30. pmc A network of nuclear envelope membrane proteins linking centromeres to microtubules
    Megan C King
    Laboratory of Cell Biology, The Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Cell 134:427-38. 2008
    ..This work highlights a framework for communication between cytoplasmic microtubules and chromatin...
  31. pmc UNC-45/CRO1/She4p (UCS) protein forms elongated dimer and joins two myosin heads near their actin binding region
    Hang Shi
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 107:21382-7. 2010
    ..We propose that a UCS dimer links two myosins at their motor domains and thereby functions as one of the determinants for step size of myosin on actin filaments...
  32. ncbi request reprint Crystal structure and mechanism of human lysine-specific demethylase-1
    Pete Stavropoulos
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA
    Nat Struct Mol Biol 13:626-32. 2006
    ..We present a biochemical analysis of LSD1 mutants that identifies crucial residues in the active site cavity and shows the importance of the SWIRM and tower domains for catalysis...
  33. pmc Structure of a myosin•adaptor complex and pairing by cargo
    Hang Shi
    Laboratory of Cell Biology and Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10065
    Proc Natl Acad Sci U S A 111:E1082-90. 2014
    ..We propose a model whereby zipcoded mRNP and/or tER ligands couple two Myo4p•She3p heterotrimers and thereby generate a transport-competent motor complex either for separate transport or cotransport of these two cargos. ..
  34. pmc Rae1 interaction with NuMA is required for bipolar spindle formation
    Richard W Wong
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 103:19783-7. 2006
    ..These data point to the Rae1-NuMA interaction as a critical element for normal spindle formation in mitosis...
  35. pmc Vesiculoviral matrix (M) protein occupies nucleic acid binding site at nucleoporin pair (Rae1•Nup98)
    Beili Quan
    Laboratory of Cell Biology and Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10065
    Proc Natl Acad Sci U S A 111:9127-32. 2014
    ..We suggest that such repetitive cycles provide cytoplasmic stopover sites required for ratcheting mRNA across the nuclear pore. ..
  36. ncbi request reprint The beta-subunit of the protein-conducting channel of the endoplasmic reticulum functions as the guanine nucleotide exchange factor for the beta-subunit of the signal recognition particle receptor
    Jurgen Helmers
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, USA
    J Biol Chem 278:23686-90. 2003
    ..when part of the isolated trimeric Sec61p complex, function as the GEF for SRbeta, whereas the same Sec61beta, when part of the heptameric complex that facilitates posttranslational protein transport, is inactive as the GEF for SRbeta..
  37. ncbi request reprint Role of nucleoporin induction in releasing an mRNA nuclear export block
    Jost Enninga
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA
    Science 295:1523-5. 2002
    ..Thus, increased Nup98 and Nup96 expression constitutes an IFN-mediated mechanism that reverses M protein-mediated inhibition of gene expression...