Mark Bedford

Summary

Affiliation: The University of Texas
Country: USA

Publications

  1. ncbi Techniques in protein methylation
    Jaeho Lee
    Deaprtment of Carcinogenesis, Science Park Research Division, The University of Texas M D Anderson Cancer Center, Smithville, TX, USA
    Methods Mol Biol 284:195-208. 2004
  2. ncbi Protein methylation and DNA repair
    Aimee N Lake
    The University of Texas MD Anderson Cancer Center, Science Park Research Division, Smithville, TX 78957, USA
    Mutat Res 618:91-101. 2007
  3. pmc Arginine methyltransferase CARM1/PRMT4 regulates endochondral ossification
    Tatsuo Ito
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    BMC Dev Biol 9:47. 2009
  4. ncbi Arginine methylation an emerging regulator of protein function
    Mark T Bedford
    Department of Carcinogenesis, The University of Texas M D Anderson Cancer Center, Smithville, Texas 78957, USA
    Mol Cell 18:263-72. 2005
  5. pmc Protein arginine methylation in mammals: who, what, and why
    Mark T Bedford
    The University of Texas M D Anderson Cancer Center, Science Park Research Division, P O Box 389, Smithville, TX 78957, USA
    Mol Cell 33:1-13. 2009
  6. ncbi Arginine methylation at a glance
    Mark T Bedford
    Department of Carcinogenesis, The University of Texas M D Anderson Cancer Center, Smithville, TX 78957, USA
    J Cell Sci 120:4243-6. 2007
  7. ncbi The arginine methyltransferase CARM1 regulates the coupling of transcription and mRNA processing
    Donghang Cheng
    The University of Texas M D Anderson Cancer Center, Science Park Research Division, P O Box 389, Smithville, TX 78957, USA
    Mol Cell 25:71-83. 2007
  8. ncbi Small molecule regulators of protein arginine methyltransferases
    Donghang Cheng
    The University of Texas M D Anderson Cancer Center, Science Park Research Division, Smithville, Texas 78957, USA
    J Biol Chem 279:23892-9. 2004
  9. ncbi Arginine methylation of the histone H3 tail impedes effector binding
    Aimee N Iberg
    The University of Texas M D Anderson Cancer Center, Science Park Research Division, Smithville, Texas 78957, USA
    J Biol Chem 283:3006-10. 2008
  10. pmc Tudor, MBT and chromo domains gauge the degree of lysine methylation
    Jeesun Kim
    The University of Texas MD Anderson Cancer Center, Science Park Research Division, Smithville, 78957, USA
    EMBO Rep 7:397-403. 2006

Collaborators

Detail Information

Publications39

  1. ncbi Techniques in protein methylation
    Jaeho Lee
    Deaprtment of Carcinogenesis, Science Park Research Division, The University of Texas M D Anderson Cancer Center, Smithville, TX, USA
    Methods Mol Biol 284:195-208. 2004
    ..Both in vivo and in vitro methylation techniques are described, and the use of small molecule inhibitors of protein methylation will be demonstrated...
  2. ncbi Protein methylation and DNA repair
    Aimee N Lake
    The University of Texas MD Anderson Cancer Center, Science Park Research Division, Smithville, TX 78957, USA
    Mutat Res 618:91-101. 2007
    ..These PTMs include phosphorylation, ubiquitination, sumoylation, acetylation, and methylation. Here we discuss recent data surrounding the roles of arginine and lysine methylation in DNA repair processes...
  3. pmc Arginine methyltransferase CARM1/PRMT4 regulates endochondral ossification
    Tatsuo Ito
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    BMC Dev Biol 9:47. 2009
    ..Here we report that coactivator-associated arginine methyltransferase 1 (CARM1) regulates chondrocyte proliferation via arginine methylation of Sox9...
  4. ncbi Arginine methylation an emerging regulator of protein function
    Mark T Bedford
    Department of Carcinogenesis, The University of Texas M D Anderson Cancer Center, Smithville, Texas 78957, USA
    Mol Cell 18:263-72. 2005
    ..This review describes recent insights into the molecular processes regulated by arginine methylation in normal and diseased cells...
  5. pmc Protein arginine methylation in mammals: who, what, and why
    Mark T Bedford
    The University of Texas M D Anderson Cancer Center, Science Park Research Division, P O Box 389, Smithville, TX 78957, USA
    Mol Cell 33:1-13. 2009
    ..Physiological roles for protein arginine methylation have been established in signal transduction, mRNA splicing, transcriptional control, DNA repair, and protein translocation...
  6. ncbi Arginine methylation at a glance
    Mark T Bedford
    Department of Carcinogenesis, The University of Texas M D Anderson Cancer Center, Smithville, TX 78957, USA
    J Cell Sci 120:4243-6. 2007
  7. ncbi The arginine methyltransferase CARM1 regulates the coupling of transcription and mRNA processing
    Donghang Cheng
    The University of Texas M D Anderson Cancer Center, Science Park Research Division, P O Box 389, Smithville, TX 78957, USA
    Mol Cell 25:71-83. 2007
    ....
  8. ncbi Small molecule regulators of protein arginine methyltransferases
    Donghang Cheng
    The University of Texas M D Anderson Cancer Center, Science Park Research Division, Smithville, Texas 78957, USA
    J Biol Chem 279:23892-9. 2004
    ..Furthermore, AMI-1 prevents in vivo arginine methylation of cellular proteins and can modulate nuclear receptor-regulated transcription from estrogen and androgen response elements, thus operating as a brake on certain hormone actions...
  9. ncbi Arginine methylation of the histone H3 tail impedes effector binding
    Aimee N Iberg
    The University of Texas M D Anderson Cancer Center, Science Park Research Division, Smithville, Texas 78957, USA
    J Biol Chem 283:3006-10. 2008
    ..We have tested the expression of a select group of H3K4 effector-regulated genes in PRMT6 knockdown cells and found that their levels are altered. Thus, PRMT6 methylates H3R2 and is a negative regulator of N-terminal H3 tail binding...
  10. pmc Tudor, MBT and chromo domains gauge the degree of lysine methylation
    Jeesun Kim
    The University of Texas MD Anderson Cancer Center, Science Park Research Division, Smithville, 78957, USA
    EMBO Rep 7:397-403. 2006
    ....
  11. pmc Xenoestrogen-induced regulation of EZH2 and histone methylation via estrogen receptor signaling to PI3K/AKT
    Tiffany G Bredfeldt
    Department of Carcinogenesis, University of Texas, M D Anderson Cancer Center, Science Park Research Division, 1808 Park Road 1C, P O Box 389, Smithville, Texas 78957, USA
    Mol Endocrinol 24:993-1006. 2010
    ..These data demonstrate that rapid ER signaling provides a direct linkage between xenoestrogen-induced nuclear hormone receptor signaling and modulation of the epigenetic machinery during tissue development...
  12. ncbi PRMT8, a new membrane-bound tissue-specific member of the protein arginine methyltransferase family
    Jaeho Lee
    Science Park Research Division, The University of Texas M D Anderson Cancer Center, Smithville, Texas 78957, USA
    J Biol Chem 280:32890-6. 2005
    ..PRMT8 is thus an active arginine methyltransferase that is membrane-associated and tissue-specific, two firsts for this family of enzymes...
  13. ncbi The novel human protein arginine N-methyltransferase PRMT6 is a nuclear enzyme displaying unique substrate specificity
    Adam Frankel
    University of Texas M D Anderson Cancer Center, Science Park Research Division, Smithville, Texas 78957, USA
    J Biol Chem 277:3537-43. 2002
    ..This novel human PRMT, which resides solely in the nucleus when fused to the green fluorescent protein, joins a family of enzymes with diverse functions within cells...
  14. ncbi Loss of CARM1 results in hypomethylation of thymocyte cyclic AMP-regulated phosphoprotein and deregulated early T cell development
    Jeesun Kim
    The University of Texas M D Anderson Cancer Center, Science Park Research Division, Smithville, Texas 78957, USA
    J Biol Chem 279:25339-44. 2004
    ..These results demonstrate that CARM1 plays a significant role in promoting the differentiation of early thymocyte progenitors, possibly through its direct action on TARPP...
  15. pmc PABP1 identified as an arginine methyltransferase substrate using high-density protein arrays
    Jaeho Lee
    The University of Texas M D Anderson Cancer Center, Science Park Research Division, PO Box 389, Smithville, TX 78957, USA
    EMBO Rep 3:268-73. 2002
    ..Prior to these findings, the only known substrate for CARM1 was histone H3. We broaden the number of CARM1 targets and suggest a role for CARM1 in regulating transcription/translation...
  16. ncbi 14-3-3 interacts with the tumor suppressor tuberin at Akt phosphorylation site(s)
    Matt Y Liu
    Department of Carcinogenesis, Science Park Research Division, The University of Texas M D Anderson Cancer Center, Smithville, Texas 78957, USA
    Cancer Res 62:6475-80. 2002
    ..Tuberin also coimmunoprecipitated with 14-3-3, confirming the interaction between endogenous 14-3-3 and tuberin. These data establish the presence of functional and overlapping 14-3-3 and Akt recognition site(s) in tuberin...
  17. pmc Ribosomal protein S2 is a substrate for mammalian PRMT3 (protein arginine methyltransferase 3)
    Rafal Swiercz
    The University of Texas M D Anderson Cancer Center, Science Park Research Division, P O Box 389, Smithville, TX 78957, USA
    Biochem J 386:85-91. 2005
    ..Furthermore, both PRMT3 and rpS2 co-sediment with free ribosomal subunits. These studies implicate PRMT3 in ribosomal function and in the regulation of protein synthesis...
  18. pmc Specific protein methylation defects and gene expression perturbations in coactivator-associated arginine methyltransferase 1-deficient mice
    Neelu Yadav
    Department of Carcinogenesis, University of Texas M D Anderson Cancer Center, P O Box 389, Smithville 78957, USA
    Proc Natl Acad Sci U S A 100:6464-8. 2003
    ..These findings provide genetic evidence for the essential role of CARM1 in estrogen-mediated transcriptional activation...
  19. pmc Enzymatic activity is required for the in vivo functions of CARM1
    Daehoon Kim
    Science Park Research Division, The University of Texas M D Anderson Cancer Center, Smithville, Texas 78957, USA
    J Biol Chem 285:1147-52. 2010
    ..CARM1 requires its enzymatic activity for all of its known cellular functions. Thus, small molecule inhibitors of CARM1 will incapacitate all of the enzyme's cellular functions...
  20. ncbi Ribosomal protein rpS2 is hypomethylated in PRMT3-deficient mice
    Rafal Swiercz
    University of Texas M D Anderson Cancer Center, Science Park Research Division, Smithville, Texas 78957, USA
    J Biol Chem 282:16917-23. 2007
    ..Finally, the levels 40 S, 60 S, and 80 S monosomes and polyribosomes are unaffected by the loss of PRMT3, but there are additional as yet unidentified proteins that co-fractionate with ribosomes that are also dedicated PRMT3 substrates...
  21. pmc CARM1 promotes adipocyte differentiation by coactivating PPARgamma
    Neelu Yadav
    Department of Carcinogenesis, The University of Texas M D Anderson Cancer Center, Science Park, Smithville, Texas 78957, USA
    EMBO Rep 9:193-8. 2008
    ..Together, these results show that CARM1 promotes adipocyte differentiation by coactivating PPARgamma-mediated transcription and thus might be important in energy balance...
  22. ncbi WWOX binds the specific proline-rich ligand PPXY: identification of candidate interacting proteins
    John H Ludes-Meyers
    Department of Carcinogenesis, University of Texas, MD Anderson Cancer Center, Science Park Research Division, PO Box 389, Smithville, TX 78957 0389, USA
    Oncogene 23:5049-55. 2004
    ..Identification and characterization of WWOX interacting proteins will lead to an understanding of the biological functions of WWOX in normal and tumor cells...
  23. doi AMPK-mediated phosphorylation of murine p27 at T197 promotes binding of 14-3-3 proteins and increases p27 stability
    John D Short
    Department of Carcinogenesis, The University of Texas M D Anderson Cancer Center Research Division, Smithville, Texas 78957, USA
    Mol Carcinog 49:429-39. 2010
    ....
  24. ncbi Regulation of protein arginine methyltransferase 8 (PRMT8) activity by its N-terminal domain
    Joyce Sayegh
    Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, California 90095 1569, USA
    J Biol Chem 282:36444-53. 2007
    ..We suggest that the N-terminal domain may function as an autoregulator that may be displaced by interaction with one or more physiological inducers...
  25. ncbi Synthesis and biological validation of novel synthetic histone/protein methyltransferase inhibitors
    Antonello Mai
    Istituto Pasteur Fondazione Cenci Bolognetti, Dipartimento di Studi Farmaceutici, Universita degli Studi di Roma La Sapienza, P le A Moro 5, 00185 Roma, Italy
    ChemMedChem 2:987-91. 2007
  26. pmc Coactivator-associated arginine methyltransferase 1 (CARM1) is a positive regulator of the Cyclin E1 gene
    Selma El Messaoudi
    Institut de Genetique Moleculaire, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5535 Institut Fédératif de Recherche 122, Universite de Montpellier II, 34293 Montpellier, France
    Proc Natl Acad Sci U S A 103:13351-6. 2006
    ..Finally, we show that grade-3 breast tumors present coelevated mRNA levels of ACTR and CARM1, along with their transcriptional target CCNE1. All together, our results indicate that CARM1 is an important regulator of the CCNE1 gene...
  27. pmc Proteome-wide analysis in Saccharomyces cerevisiae identifies several PHD fingers as novel direct and selective binding modules of histone H3 methylated at either lysine 4 or lysine 36
    Xiaobing Shi
    Department of Biological Sciences, Stanford University, Stanford, California 94305, USA
    J Biol Chem 282:2450-5. 2007
    ..Together, our study suggests that a common function for PHD fingers is to transduce methyl-lysine events and sheds light on how a single histone modification can be linked to multiple biological outcomes...
  28. ncbi Recognition of histone H3 lysine-4 methylation by the double tudor domain of JMJD2A
    Ying Huang
    W M Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Science 312:748-51. 2006
    ..Our study provides mechanistic insights into recognition of methylated histone tails by tudor domains and reveals the structural intricacy of methyl-lysine recognition by two closely spaced effector domains...
  29. ncbi The AT-hook of the chromatin architectural transcription factor high mobility group A1a is arginine-methylated by protein arginine methyltransferase 6
    Riccardo Sgarra
    Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, University of Trieste, Italy
    J Biol Chem 281:3764-72. 2006
    ..The in vivo association of HMGA and PRMT6 place this yet functionally uncharacterized methyltransferase in the well established functional context of the chromatin structure organization...
  30. ncbi Normalization of nomenclature for peptide motifs as ligands of modular protein domains
    Rein Aasland
    Department of Molecular Biology, University of Bergen, 5020 Bergen, Norway
    FEBS Lett 513:141-4. 2002
    ..This proposal will be reviewed in the future and will therefore be open for the inclusion of new rules, modifications and changes...
  31. pmc A chromatin-wide transition to H4K20 monomethylation impairs genome integrity and programmed DNA rearrangements in the mouse
    Gunnar Schotta
    Research Institute of Molecular Pathology IMP, The Vienna Biocenter, A 1030 Vienna, Austria
    Genes Dev 22:2048-61. 2008
    ..Thus, conversion to an H4K20me1 state results in compromised chromatin that is insufficient to protect genome integrity and to process a DNA-rearranging differentiation program in the mouse...
  32. doi epigenetic multiple ligands: mixed histone/protein methyltransferase, acetyltransferase, and class III deacetylase (sirtuin) inhibitors
    Antonello Mai
    Istituto Pasteur Fondazione Cenci Bolognetti, Dipartimento di Studi Farmaceutici, Universita degli Studi di Roma La Sapienza, Piazzale A Moro 5, 00185 Rome, Italy
    J Med Chem 51:2279-90. 2008
    ..e., 40.7% ( 4l) and 42.6% ( 7b)] and/or massive, dose-dependent cytodifferentiation [i.e., 95.2% ( 1c) and 96.1% ( 4j)], whereas the single-target inhibitors eosin, curcumin, and sirtinol were ineffective or showed a weak effect...
  33. doi Protein arginine methyltransferase 1 coactivates NF-kappaB-dependent gene expression synergistically with CARM1 and PARP1
    Paul O Hassa
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    J Mol Biol 377:668-78. 2008
    ..These results suggest that the cooperative action between PRMT1 and CARM1 is required for NF-kappaB-dependent gene expression...
  34. pmc Sam68 RNA binding protein is an in vivo substrate for protein arginine N-methyltransferase 1
    Jocelyn Cote
    Sir Mortimer B Davis Jewish General Hospital, Department of Oncology, McGill University, Montreal, Quebec, H3T 1E2 Canada
    Mol Biol Cell 14:274-87. 2003
    ..These findings demonstrate that RNA binding proteins are in vivo substrates for PRMT1, and their methylation is essential for their proper localization and function...
  35. ncbi p53 is regulated by the lysine demethylase LSD1
    Jing Huang
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Nature 449:105-8. 2007
    ..Lysine methylation therefore provides similar regulatory complexity for non-histone proteins and for histones...
  36. ncbi Reprogramming the histone code
    Salam A Shaaban
    OSI Pharmaceuticals Inc, 1 Bioscience Park Drive, Farmingdale, NY 11735, USA
    Chem Biol 14:242-4. 2007
  37. ncbi Dynamics of human protein arginine methyltransferase 1(PRMT1) in vivo
    Frank Herrmann
    Department of Molecular Cell Biology, Heinrich Pette Institute, Hamburg, Germany
    J Biol Chem 280:38005-10. 2005
    ....
  38. pmc Methylation of Tat by PRMT6 regulates human immunodeficiency virus type 1 gene expression
    Marie Chloe Boulanger
    Terry Fox Molecular Oncology Group, Lady Davis Institute, Sir Mortimer B Davis Jewish General Hospital, Montreal, Quebec, Canada
    J Virol 79:124-31. 2005
    ..Our study demonstrates that arginine methylation of Tat negatively regulates its transactivation activity and that PRMT6 acts as a restriction factor for HIV replication...
  39. pmc Arginine methyltransferase CARM1 is a promoter-specific regulator of NF-kappaB-dependent gene expression
    Marcela Covic
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, Zurich, Switzerland
    EMBO J 24:85-96. 2005
    ..Our results suggest that the cooperative action between protein arginine methyltransferases and protein lysine acetyltransferases regulates NF-kappaB-dependent gene activation in vivo...

Research Grants16

  1. The Functional Analysis of the Coactivator CARM1
    Mark Bedford; Fiscal Year: 2007
    ..abstract_text> ..
  2. The Functional Analysis of the Coactivator CARM1
    Mark Bedford; Fiscal Year: 2007
    ..Ultimately, this in-depth analysis of CARM1 function will lead to the mechanistic understanding of a novel class of deregulated enzymes that play a role in a number of disease states and are new targets for drug development. ..
  3. Regulation of Transcriptional Coactivators by Environmental Estrogens (R21)
    Mark Bedford; Fiscal Year: 2007
    ..The specific aims are to characterize: 1) Biochemically xenoestrogens as co-activator inhibitors; and 2) In vitro xenoestrogens as co-activator inhibitors. ..
  4. Identifying and Characterizing Readers of the Neural Histone Code
    Mark Bedford; Fiscal Year: 2007
    ..In this study we plan to investigate how this memory code is read. This basic scientific study will clearly impact the fields of drug addiction, mental retardation and aging diseases of the brain. ..
  5. Small Molecule Regulators of Arginine Methyltransferases
    Mark Bedford; Fiscal Year: 2007
    ..Most importantly, the PRMTs are a newly identified family of enzymes that have not yet been tapped as "drugable" targets. ..
  6. The Functional Analysis of the Coactivator CARM1
    Mark Bedford; Fiscal Year: 2005
    ..abstract_text> ..
  7. Protein Array Technology for Protein Interaction
    Mark Bedford; Fiscal Year: 2003
    ..In addition, this type of protein-domain array will facilitate the identification of risk factors and chemoprevention targets at the protein level. ..
  8. The Functional Analysis of the Coactivator CARM1
    Mark T Bedford; Fiscal Year: 2010
    ..Ultimately, this in-depth analysis of CARM1 function will lead to the mechanistic understanding of a novel class of deregulated enzymes that play a role in a number of disease states and are new targets for drug development. ..