Francisco Asturias

Summary

Affiliation: The Scripps Research Institute
Country: USA

Publications

  1. pmc Structural organization of yeast and mammalian mediator complexes
    M R Dotson
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 97:14307-10. 2000
  2. ncbi request reprint Mammalian fatty acid synthase: X-ray structure of a molecular assembly line
    Francisco J Asturias
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    ACS Chem Biol 1:135-8. 2006
  3. ncbi request reprint Structure of Saccharomyces cerevisiae DNA polymerase epsilon by cryo-electron microscopy
    Francisco J Asturias
    Department of Structural Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Struct Mol Biol 13:35-43. 2006
  4. ncbi request reprint Structure and molecular organization of mammalian fatty acid synthase
    Francisco J Asturias
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Struct Mol Biol 12:225-32. 2005
  5. ncbi request reprint RNA polymerase II structure, and organization of the preinitiation complex
    Francisco J Asturias
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Curr Opin Struct Biol 14:121-9. 2004
  6. pmc Structural analysis of the RSC chromatin-remodeling complex
    Francisco J Asturias
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 99:13477-80. 2002
  7. pmc RNA polymerase II at initiation
    Francisco J Asturias
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, CB227, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 100:6893-5. 2003
  8. ncbi request reprint Head module control of mediator interactions
    Yuichiro Takagi
    Department of Structural Biology, Stanford University School of Medicine, Stanford, California 94305, USA
    Mol Cell 23:355-64. 2006
  9. pmc Mediator structural conservation and implications for the regulation mechanism
    Gang Cai
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Structure 17:559-67. 2009
  10. ncbi request reprint Electron microscopic analysis of the RSC chromatin remodeling complex
    Francisco J Asturias
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Methods Enzymol 376:48-62. 2004

Research Grants

  1. EUKARYOTIC TRANSCRIPTION INITIATION COMPLEX STRUCTURE
    Francisco Asturias; Fiscal Year: 2006
  2. STRUCTURAL STUDY OF EUKARYOTIC TRANSCRIPTION REGULATION
    Francisco Asturias; Fiscal Year: 2004
  3. Structural Studies of Eukaryotic Transcription
    Francisco J Asturias; Fiscal Year: 2010

Collaborators

Detail Information

Publications21

  1. pmc Structural organization of yeast and mammalian mediator complexes
    M R Dotson
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 97:14307-10. 2000
    ..Biochemically defined Rgr1 and Srb4 modules of yeast Mediator are then assigned to the middle and head domains...
  2. ncbi request reprint Mammalian fatty acid synthase: X-ray structure of a molecular assembly line
    Francisco J Asturias
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    ACS Chem Biol 1:135-8. 2006
    ..Further analysis with techniques capable of providing information about single molecule conformations will eventually provide a more complete understanding of FAS...
  3. ncbi request reprint Structure of Saccharomyces cerevisiae DNA polymerase epsilon by cryo-electron microscopy
    Francisco J Asturias
    Department of Structural Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Struct Mol Biol 13:35-43. 2006
    ..Together, these observations suggest a mechanism for interaction of Pol epsilon with DNA that might explain how the structure of the enzyme contributes to its intrinsic processivity...
  4. ncbi request reprint Structure and molecular organization of mammalian fatty acid synthase
    Francisco J Asturias
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Struct Mol Biol 12:225-32. 2005
    ..The new FAS structure provides a new paradigm for understanding the architecture of FAS and the related modular polyketide synthases...
  5. ncbi request reprint RNA polymerase II structure, and organization of the preinitiation complex
    Francisco J Asturias
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Curr Opin Struct Biol 14:121-9. 2004
    ..The model provides insight into the possible mechanisms of transcription initiation and regulation...
  6. pmc Structural analysis of the RSC chromatin-remodeling complex
    Francisco J Asturias
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 99:13477-80. 2002
    ..Results of nuclease protection analysis are consistent with nucleosome binding in the cavity. Such binding could explain the ability of RSC to expose nucleosomal DNA in the presence of ATP without loss of associated histones...
  7. pmc RNA polymerase II at initiation
    Francisco J Asturias
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, CB227, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 100:6893-5. 2003
  8. ncbi request reprint Head module control of mediator interactions
    Yuichiro Takagi
    Department of Structural Biology, Stanford University School of Medicine, Stanford, California 94305, USA
    Mol Cell 23:355-64. 2006
    ..The head module evidently controls Mediator-RNA polymerase II and Mediator-promoter interactions...
  9. pmc Mediator structural conservation and implications for the regulation mechanism
    Gang Cai
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Structure 17:559-67. 2009
    ..This suggests that the topology and structural dynamics of Mediator constitute important elements of a conserved regulation mechanism...
  10. ncbi request reprint Electron microscopic analysis of the RSC chromatin remodeling complex
    Francisco J Asturias
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Methods Enzymol 376:48-62. 2004
  11. ncbi request reprint RNA polymerase II/TFIIF structure and conserved organization of the initiation complex
    Wen Hsiang Chung
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Mol Cell 12:1003-13. 2003
    ..The structure of the RNAPII/TFIIF complex suggests a model for the organization of a minimal transcription initiation complex...
  12. doi request reprint Single-particle electron microscopy of animal fatty acid synthase describing macromolecular rearrangements that enable catalysis
    Edward J Brignole
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California, USA
    Methods Enzymol 483:179-202. 2010
    ....
  13. ncbi request reprint Preponderance of free mediator in the yeast Saccharomyces cerevisiae
    Yuichiro Takagi
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 280:31200-7. 2005
    ....
  14. pmc Mediator head module structure and functional interactions
    Gang Cai
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California, USA
    Nat Struct Mol Biol 17:273-9. 2010
    ....
  15. pmc Structure of a RSC-nucleosome complex and insights into chromatin remodeling
    Yuriy Chaban
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Struct Mol Biol 15:1272-7. 2008
    ..Consideration of the RSC-nucleosome structure and published biochemical information suggests that ATP-dependent DNA translocation by RSC may result in the eviction of histone octamers from adjacent nucleosomes...
  16. ncbi request reprint Structure of eukaryotic Mediator complexes
    James Z Chadick
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road CB227, La Jolla, CA 92037, USA
    Trends Biochem Sci 30:264-71. 2005
    ..The results reveal how Mediator interacts with RNA polymerase II, and suggest that regulatory information could be conveyed through changes in Mediator conformation that would influence the transcription initiation process...
  17. pmc Conformational flexibility of metazoan fatty acid synthase enables catalysis
    Edward J Brignole
    Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Struct Mol Biol 16:190-7. 2009
    ..Concomitant rearrangement of the beta-carbon-processing domains synchronizes acyl chain reduction in one chamber with acyl chain elongation in the other...
  18. ncbi request reprint Structure of yeast RNA polymerase II in solution: implications for enzyme regulation and interaction with promoter DNA
    John L Craighead
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Structure 10:1117-25. 2002
    ..The RNAPII structure suggests a revised mode of interaction with promoter DNA and demonstrates that regulation of RNAPII must involve structural changes that render the enzyme competent for initiation...
  19. ncbi request reprint A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II
    Henri Marc Bourbon
    Mol Cell 14:553-7. 2004
  20. ncbi request reprint Another piece in the transcription initiation puzzle
    Francisco J Asturias
    Nat Struct Mol Biol 11:1031-3. 2004
  21. ncbi request reprint Head-to-head coiled arrangement of the subunits of the animal fatty acid synthase
    Andrzej Witkowski
    Children s Hospital Oakland Research Institute, 5700 Martin Luther King Jr Way, Oakland, California 94609, USA
    Chem Biol 11:1667-76. 2004
    ....

Research Grants11

  1. EUKARYOTIC TRANSCRIPTION INITIATION COMPLEX STRUCTURE
    Francisco Asturias; Fiscal Year: 2006
    ..The structures will indicate the way in which regulatory information is transduced to the preinitiation complex, and reveal the structural underpinnings of the transcription regulation mechanism in eukaryotic cells. ..
  2. STRUCTURAL STUDY OF EUKARYOTIC TRANSCRIPTION REGULATION
    Francisco Asturias; Fiscal Year: 2004
    ..By identifying structural differences and similarities between the complexes we hope to contribute to the understanding of what increasingly appears as a universal mechanism for transcription control. ..
  3. Structural Studies of Eukaryotic Transcription
    Francisco J Asturias; Fiscal Year: 2010
    ..The causal relationship between problems with transcription regulation and many diseases (notably cancer) explains the significance of these studies to public health. ..