John H Wilson

Summary

Affiliation: Texas Medical Center
Country: USA

Publications

  1. ncbi request reprint Activation of gene expression by triplex-directed psoralen crosslinks
    Jie Song
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Gene 324:183-90. 2004
  2. pmc Knock-in human rhodopsin-GFP fusions as mouse models for human disease and targets for gene therapy
    Fung Chan
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 101:9109-14. 2004
  3. ncbi request reprint The nature of dominant mutations of rhodopsin and implications for gene therapy
    John H Wilson
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Mol Neurobiol 28:149-58. 2003
  4. pmc Mislocalization and degradation of human P23H-rhodopsin-GFP in a knockin mouse model of retinitis pigmentosa
    Brandee A Price
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Invest Ophthalmol Vis Sci 52:9728-36. 2011
  5. ncbi request reprint Rhodopsin-EGFP knock-ins for imaging quantal gene alterations
    Theodore G Wensel
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Vision Res 45:3445-53. 2005
  6. pmc Rhodopsin gene expression determines rod outer segment size and rod cell resistance to a dominant-negative neurodegeneration mutant
    Brandee A Price
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas, United States of America
    PLoS ONE 7:e49889. 2012
  7. ncbi request reprint Defective development of photoreceptor membranes in a mouse model of recessive retinal degeneration
    Alecia K Gross
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Vision Res 46:4510-8. 2006
  8. pmc Topoisomerase 1 and single-strand break repair modulate transcription-induced CAG repeat contraction in human cells
    Leroy Hubert
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Mol Cell Biol 31:3105-12. 2011
  9. pmc Xpa deficiency reduces CAG trinucleotide repeat instability in neuronal tissues in a mouse model of SCA1
    Leroy Hubert
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Hum Mol Genet 20:4822-30. 2011
  10. pmc Impaired photoreceptor protein transport and synaptic transmission in a mouse model of Bardet-Biedl syndrome
    Muhammad M Abd-El-Barr
    Department of Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA
    Vision Res 47:3394-407. 2007

Collaborators

Detail Information

Publications23

  1. ncbi request reprint Activation of gene expression by triplex-directed psoralen crosslinks
    Jie Song
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Gene 324:183-90. 2004
    ..Collectively, these results demonstrate that TFOs can be used to direct psoralen crosslinks adjacent to a gene as a way of activating gene expression...
  2. pmc Knock-in human rhodopsin-GFP fusions as mouse models for human disease and targets for gene therapy
    Fung Chan
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 101:9109-14. 2004
    ....
  3. ncbi request reprint The nature of dominant mutations of rhodopsin and implications for gene therapy
    John H Wilson
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Mol Neurobiol 28:149-58. 2003
    ..If added wild-type rhodopsin could slow retinal degeneration in human patients, as it does in mice, it would represent a valuable new strategy for gene therapy of RP caused by dominant rhodopsin mutations...
  4. pmc Mislocalization and degradation of human P23H-rhodopsin-GFP in a knockin mouse model of retinitis pigmentosa
    Brandee A Price
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Invest Ophthalmol Vis Sci 52:9728-36. 2011
    ..The goal was to introduce a gene that expressed rhodopsin at low levels to avoid rapid retinal degeneration, and with a readily visible tag to make it easy to distinguish from wild type rhodopsin...
  5. ncbi request reprint Rhodopsin-EGFP knock-ins for imaging quantal gene alterations
    Theodore G Wensel
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Vision Res 45:3445-53. 2005
    ..They represent tools for studying molecular triggers of photoreceptor development, for following stem cell populations, and for evaluating retinal transplantation experiments...
  6. pmc Rhodopsin gene expression determines rod outer segment size and rod cell resistance to a dominant-negative neurodegeneration mutant
    Brandee A Price
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas, United States of America
    PLoS ONE 7:e49889. 2012
    ..These results point to promising directions in gene therapy for autosomal dominant neurodegenerative diseases caused by dominant-negative mutations...
  7. ncbi request reprint Defective development of photoreceptor membranes in a mouse model of recessive retinal degeneration
    Alecia K Gross
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Vision Res 46:4510-8. 2006
    ..These results reveal that in addition to its role in transport and sorting of rhodopsin to disk membranes, rhodopsin is also essential for formation of disks...
  8. pmc Topoisomerase 1 and single-strand break repair modulate transcription-induced CAG repeat contraction in human cells
    Leroy Hubert
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Mol Cell Biol 31:3105-12. 2011
    ..These studies broaden the scope of pathways involved in transcription-induced CAG repeat instability and begin to define their interrelationships...
  9. pmc Xpa deficiency reduces CAG trinucleotide repeat instability in neuronal tissues in a mouse model of SCA1
    Leroy Hubert
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Hum Mol Genet 20:4822-30. 2011
    ..These results validate our original findings in cultured human cells and suggest that transcription may induce NER-dependent TNR instability in neuronal tissues in humans...
  10. pmc Impaired photoreceptor protein transport and synaptic transmission in a mouse model of Bardet-Biedl syndrome
    Muhammad M Abd-El-Barr
    Department of Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA
    Vision Res 47:3394-407. 2007
    ..Additionally, we show defects in synaptic transmission from the photoreceptors to secondary neurons of the visual system, demonstrating multiple functions for BBS4 in photoreceptors...
  11. pmc Efficient mutagenesis of the rhodopsin gene in rod photoreceptor neurons in mice
    Fung Chan
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Nucleic Acids Res 39:5955-66. 2011
    ..These studies establish that the genomes of terminally differentiated rod cells can be efficiently edited in living organisms...
  12. pmc Transcription-induced DNA toxicity at trinucleotide repeats: double bubble is trouble
    Yunfu Lin
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX USA
    Cell Cycle 10:611-8. 2011
    ..Transcription-induced DNA toxicity may have profound biological consequences, with particular relevance to repeat-associated neurodegenerative diseases...
  13. pmc R loops stimulate genetic instability of CTG.CAG repeats
    Yunfu Lin
    Baylor College of Medicine, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, One Baylor Plaza, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 107:692-7. 2010
    ..DNA hybrid. These studies demonstrate that persistent hybrids between the nascent RNA transcript and the template DNA strand at CTG.CAG tracts promote instability of DNA trinucleotide repeats...
  14. pmc Hsp90 modulates CAG repeat instability in human cells
    David Mittelman
    Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Cell Stress Chaperones 15:753-9. 2010
    ..The capacity of Hsp90 to modulate repeat-tract lengths suggests that the chaperone, in addition to exposing cryptic variation, might facilitate the expression of new phenotypes through induction of novel genetic variation...
  15. pmc Nucleotide excision repair, mismatch repair, and R-loops modulate convergent transcription-induced cell death and repeat instability
    Yunfu Lin
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas, United States of America
    PLoS ONE 7:e46807. 2012
    ..These observations link the mechanisms of convergent transcription-induced repeat instability and convergent transcription-induced cell death, suggesting that a common structure may trigger both outcomes...
  16. pmc Convergent transcription through a long CAG tract destabilizes repeats and induces apoptosis
    Yunfu Lin
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Mol Cell Biol 30:4435-51. 2010
    ..Convergent transcription through a CAG repeat represents a novel mechanism for triggering a cellular stress response, one that is initiated by events at a single locus in the genome and resembles the response to DNA damage...
  17. pmc Transcription destabilizes triplet repeats
    Yunfu Lin
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Mol Carcinog 48:350-61. 2009
    ....
  18. pmc Evaluating retinal toxicity of intravitreal caspofungin in the mouse eye
    Deb K Mojumder
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Invest Ophthalmol Vis Sci 51:5796-803. 2010
    ..In this study, retinal toxicity was determined after intravitreal injection of caspofungin in a mouse model to assess its safety profile for the treatment of fungal endophthalmitis...
  19. pmc Stress, genomes, and evolution
    David Mittelman
    Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Cell Stress Chaperones 15:463-6. 2010
    ..We discuss key findings that suggest a rich set of pathways by which Hsp90 can mediate the influences of the environment on the genome...
  20. pmc Repair of chromosomal double-strand breaks by precise ligation in human cells
    William Y Lin
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    DNA Repair (Amst) 12:480-7. 2013
    ..In summary, our results demonstrate that DSBs in human cells are efficiently repaired by precise ligation, which requires classical NHEJ components and is enhanced by p53 and BRCA1...
  21. pmc Zinc-finger directed double-strand breaks within CAG repeat tracts promote repeat instability in human cells
    David Mittelman
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology and Graduate Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 106:9607-12. 2009
    ..These studies identify a ZFN as a useful reagent for characterizing the effects of DSBs on CAG repeats in cells...
  22. pmc Neural reprogramming in retinal degeneration
    Robert E Marc
    Department of Ophthalmology, John A Moran Eye Center, University of Utah, Salt Lake City, Utah 84132
    Invest Ophthalmol Vis Sci 48:3364-71. 2007
    ..The authors sought to explore the functional expression of ionotropic (iGluR) and group 3, type 6 metabotropic (mGluR6) glutamate receptors in late-stage photoreceptor degeneration...
  23. ncbi request reprint [Triple helix: a new promise for gene therapy]
    Zsofia Intody
    Semmelweis Egyetem, Általános Orvostudományi Kar, I Sz Szemészeti Klinika, Budapest
    Orv Hetil 144:757-63. 2003
    ..The most common cause for autosomal dominant retinitis pigmentosa is a mutation in the rhodopsin gene...

Research Grants41

  1. Instability of Triplet Repeats in Mammalian Cells
    John H Wilson; Fiscal Year: 2010
    ..These studies will define basic mechanisms of repeat instability in germline and somatic tissues, test a novel mechanism of disease pathogenesis, and develop a reagent with potential therapeutic applications. ..
  2. Rhodopsin Gene Correction and Gene Knockout in Rod Cells
    John Wilson; Fiscal Year: 2007
    ..Assays for rhodopsin gene correction and knockout will utilize the fluorescent properties of the human rhodopsin-GFP target gene. ..
  3. Instability of Triplet Repeats in Mammalian Cells
    John Wilson; Fiscal Year: 2006
    ..abstract_text> ..
  4. RHODOSPIN GENE CORRECTION BY OLIGONUCLEOTIDE TARGETING
    John Wilson; Fiscal Year: 2005
    ..These results will be used as the basis for developing treatment protocols in mice. ..
  5. Instability of Triplet Repeats in Mammalian Cells
    John Wilson; Fiscal Year: 2007
    ..Our goal is to delineate those processes that are responsible for both the germline and somatic CTG/CAG repeat instability that characterizes myotonic dystrophy and other neurological diseases. ..
  6. Instability of Triplet Repeats in Mammalian Cells
    John Wilson; Fiscal Year: 2006
    ..Our goal is to delineate those processes that are responsible for both the germline and somatic CTG/CAG repeat instability that characterizes myotonic dystrophy and other neurological diseases. ..
  7. Instability of Triplet Repeats in Mammalian Cells
    John Wilson; Fiscal Year: 2009
    ..Our goal is to delineate those processes that are responsible for both the germline and somatic CTG/CAG repeat instability that characterizes myotonic dystrophy and other neurological diseases. ..
  8. Rhodopsin Gene Correction and Gene Knockout in Rod Cells
    John H Wilson; Fiscal Year: 2010
    ..Assays for rhodopsin gene correction and knockout will utilize the fluorescent properties of the humanrhodopsin-GFPtarget gene. ..
  9. Rhodopsin Gene Correction and Gene Knockout in Rod Cells
    John Wilson; Fiscal Year: 2009
    ..Assays for rhodopsin gene correction and knockout will utilize the fluorescent properties of the humanrhodopsin-GFPtarget gene. ..
  10. Rhodopsin Gene Correction and Gene Knockout in Rod Cells
    John Wilson; Fiscal Year: 2007
    ..Assays for rhodopsin gene correction and knockout will utilize the fluorescent properties of the humanrhodopsin-GFPtarget gene. ..
  11. RHODOPSIN GENE CORRECTION BY OLIGONUCLEOTIDE TARGETING
    John Wilson; Fiscal Year: 1999
    ..This will be done by fusing the Green fluorescent Protein (GFP) to the rhodopsin gene. This construct will allow fluorescent-activated cell sorting to be used to collect altered cells. ..
  12. TARGETED RECOMBINATION IN CELLS
    John Wilson; Fiscal Year: 2000
    ..Considering what is known about ERCC-1 function, a number of questions can be put forth. For example, the effect of nonhomologous tails could be quite interesting as could be triplex formation under repair-defective states. ..
  13. TARGETED RECOMBINATION IN MAMMALIAN CELLS
    John Wilson; Fiscal Year: 1993
    ..Collectively, these studies will advance understanding of mammalian gene targeting and may lead to improved targeting frequencies...
  14. TARGETED RECOMBINATION IN MAMMALIAN CELLS
    John Wilson; Fiscal Year: 1991
    ....