Mark E Westhusin

Summary

Affiliation: Texas A and M University
Country: USA

Publications

  1. pmc Production of transgenic calves expressing an shRNA targeting myostatin
    K Tessanne
    Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A and M University, College Station, Texas, USA
    Mol Reprod Dev 79:176-85. 2012
  2. ncbi request reprint Rescuing valuable genomes by animal cloning: a case for natural disease resistance in cattle
    M E Westhusin
    Texas A and M University, College of Veterinary Medicine and Biomedical Sciences, College Station, TX, USA
    J Anim Sci 85:138-42. 2007
  3. ncbi request reprint Cloning to reproduce desired genotypes
    M E Westhusin
    Departments of Veterinary Physiology and Pharmacology, Anatomy and Public Health, College of Veterinary Medicine, Texas A and M University, College Station, TX 77843, USA
    Theriogenology 55:35-49. 2001
  4. doi request reprint Effects of early pregnancy diagnosis by palpation per rectum on pregnancy loss in dairy cattle
    Juan E Romano
    Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX 77843, USA
    J Am Vet Med Assoc 239:668-73. 2011
  5. ncbi request reprint Early pregnancy diagnosis by palpation per rectum: influence on embryo/fetal viability in dairy cattle
    Juan E Romano
    Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX 77845, USA
    Theriogenology 67:486-93. 2007
  6. pmc Examination of DNA methyltransferase expression in cloned embryos reveals an essential role for Dnmt1 in bovine development
    Michael C Golding
    Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX 77843 4466, USA
    Mol Reprod Dev 78:306-17. 2011
  7. ncbi request reprint Cloning of GJA1 (connexin43) and its expression in canine ovarian follicles throughout the estrous cycle
    Lauri A Willingham-Rocky
    Department of Veterinary Integrative Biosciences, Texas A and M University, College Station, TX, USA
    Gene Expr Patterns 7:66-71. 2007
  8. ncbi request reprint Cloning of exotic/endangered species: desert bighorn sheep
    James Buck Williams
    Departmetn of Veterinary Physiology and Pharmacology, Texas A and M University, College Station, USA
    Methods Mol Biol 348:169-82. 2006
  9. ncbi request reprint A simple salt solution medium supplemented with yolk plasma and lactate (YPLM) supports development of preimplantation bovine embryos in vitro
    Y M Elhassan
    Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A and M University, College Station 77843 4466, USA
    Anim Reprod Sci 57:153-66. 1999
  10. ncbi request reprint Effects of stage of oestrous cycle and progesterone supplementation during culture on maturation of canine oocytes in vitro
    L A Willingham-Rocky
    Department of Veterinary Physiology and Pharmacology, Texas A and M University, College of Veterinary Medicine, College Station, Texas 77843 4466, USA
    Reproduction 126:501-8. 2003

Research Grants

Detail Information

Publications25

  1. pmc Production of transgenic calves expressing an shRNA targeting myostatin
    K Tessanne
    Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A and M University, College Station, Texas, USA
    Mol Reprod Dev 79:176-85. 2012
    ..This is the first report of transgenic livestock produced by direct injection of a recombinant lentivirus, and expressing transgenes encoding shRNAs targeting an endogenous gene (myostatin) for silencing...
  2. ncbi request reprint Rescuing valuable genomes by animal cloning: a case for natural disease resistance in cattle
    M E Westhusin
    Texas A and M University, College of Veterinary Medicine and Biomedical Sciences, College Station, TX, USA
    J Anim Sci 85:138-42. 2007
    ..Furthermore, in vitro assays performed to date indicate this bull is naturally resistant to B. abortus, Mycobacterium bovis, and Salmonella typhimurium, as was the original genetic donor...
  3. ncbi request reprint Cloning to reproduce desired genotypes
    M E Westhusin
    Departments of Veterinary Physiology and Pharmacology, Anatomy and Public Health, College of Veterinary Medicine, Texas A and M University, College Station, TX 77843, USA
    Theriogenology 55:35-49. 2001
    ..The ability to reproduce any desired genotype by cloning will ultimately depend on the amount of time and resources invested in research...
  4. doi request reprint Effects of early pregnancy diagnosis by palpation per rectum on pregnancy loss in dairy cattle
    Juan E Romano
    Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX 77843, USA
    J Am Vet Med Assoc 239:668-73. 2011
    ..To determine the effect of palpation per rectum (PPR) by use of 1 or 2 fetal membrane slips (FMSs) for pregnancy diagnosis during early gestation on pregnancy loss in dairy cattle...
  5. ncbi request reprint Early pregnancy diagnosis by palpation per rectum: influence on embryo/fetal viability in dairy cattle
    Juan E Romano
    Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX 77845, USA
    Theriogenology 67:486-93. 2007
    ..025), but was not different (P>0.05) between PAL (14.7%; 38/258) and NPAL (13.4%; 35/262). In conclusion, PPR between days 34 and 41 of pregnancy using the fetal membrane slip technique did not affect embryo/fetal viability...
  6. pmc Examination of DNA methyltransferase expression in cloned embryos reveals an essential role for Dnmt1 in bovine development
    Michael C Golding
    Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, TX 77843 4466, USA
    Mol Reprod Dev 78:306-17. 2011
    ..Our results indicate an essential role for Dnmt1 during bovine preimplantation development, and suggest proper transcriptional reprogramming of this gene family in SCNT embryos...
  7. ncbi request reprint Cloning of GJA1 (connexin43) and its expression in canine ovarian follicles throughout the estrous cycle
    Lauri A Willingham-Rocky
    Department of Veterinary Integrative Biosciences, Texas A and M University, College Station, TX, USA
    Gene Expr Patterns 7:66-71. 2007
    ....
  8. ncbi request reprint Cloning of exotic/endangered species: desert bighorn sheep
    James Buck Williams
    Departmetn of Veterinary Physiology and Pharmacology, Texas A and M University, College Station, USA
    Methods Mol Biol 348:169-82. 2006
    ..Animal care and welfare issues raised that are unique to exotic and endangered species also are raised. In this chapter, the methods used in attempts to clone the wild animal species of Desert Bighorn Sheep are described...
  9. ncbi request reprint A simple salt solution medium supplemented with yolk plasma and lactate (YPLM) supports development of preimplantation bovine embryos in vitro
    Y M Elhassan
    Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A and M University, College Station 77843 4466, USA
    Anim Reprod Sci 57:153-66. 1999
    ..In conclusion, YP, with the advantage of being clearer than EY, is capable of sustaining embryo development to the blastocyst stage in a simple salt solution of NaCl, KCl and NaHCO3 supplemented with hemicalcium L-lactate...
  10. ncbi request reprint Effects of stage of oestrous cycle and progesterone supplementation during culture on maturation of canine oocytes in vitro
    L A Willingham-Rocky
    Department of Veterinary Physiology and Pharmacology, Texas A and M University, College of Veterinary Medicine, College Station, Texas 77843 4466, USA
    Reproduction 126:501-8. 2003
    ..However, stage of oestrous cycle is a key factor in the selection criteria for meiotically competent canine oocytes for use in in vitro experiments...
  11. ncbi request reprint Production of nuclear transfer horse embryos by Piezo-driven injection of somatic cell nuclei and activation with stallion sperm cytosolic extract
    Y H Choi
    Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A and M University, College Station, TX 77843, USA
    Biol Reprod 67:561-7. 2002
    ..that direct nuclear injection using the Piezo drill is an efficient method for nuclear transfer in horse and cattle oocytes and that sperm extract can efficiently activate horse oocytes both parthenogenetically and after nuclear transfer..
  12. ncbi request reprint Amino acid concentrations in fluids from the bovine oviduct and uterus and in KSOM-based culture media
    Y M Elhassan
    Department of Veterinary Physiology and Pharmacology, Texas A and M University, College Station 77843 2471, USA
    Theriogenology 55:1907-18. 2001
    ..The particular pattern of amino acid concentrations may be an important factor to be considered for the improvement of embryo culture media...
  13. ncbi request reprint Potential for cloning dogs
    M E Westhusin
    Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A and M University, College Station, TX 77845, USA
    J Reprod Fertil Suppl 57:287-93. 2001
    ..However, significant investment into additional research is required, especially in the areas of in vitro maturation of oocytes and control of the oestrous cycle of bitches...
  14. ncbi request reprint Development rates of male bovine nuclear transfer embryos derived from adult and fetal cells
    J R Hill
    Department of Veterinary Physiology and Pharmacology, Department of Large Animal Medicine and Surgery, Texas A and M University, College Station, Texas 77843, USA
    Biol Reprod 62:1135-40. 2000
    ..Serum starvation of these adult donor cells did not improve development rates of NT embryos to blastocyst, but when fetal cells were serum-starved, there was a significant increase in development to blastocyst...
  15. ncbi request reprint In vitro development of equine nuclear transfer embryos: effects of oocyte maturation media and amino acid composition during embryo culture
    Y H Choi
    Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A and M University, College Station 77843 4466, USA
    Zygote 11:77-86. 2003
    ..CZB-C medium may be used for culture of equine embryos with results similar to those obtained with G1.2 medium, thus providing a base medium that may be modified for further study of culture requirements of equine embryos...
  16. ncbi request reprint Bovine nuclear transfer embryo development using cells derived from a cloned fetus
    J R Hill
    Department of Veterinary Physiology and Pharmacology, Texas A and M University, College Station, TX 77843 4466, USA
    Anim Reprod Sci 67:17-26. 2001
    ..Further studies on this and other recloned fetuses are necessary to determine whether the failure to produce live offspring was a result of inadequate sample size or due to the cell type selected...
  17. ncbi request reprint Role of cumulus cells on in vitro maturation of canine oocytes
    T Otoi
    Department of Physiology and Pharmacology, College of Veterinary Medicine, Texas A and M University, College Station, TX, USA
    Reprod Domest Anim 42:184-9. 2007
    ....
  18. pmc Reshaping the transcriptional frontier: epigenetics and somatic cell nuclear transfer
    Charles R Long
    Department of Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A and M University, College Station, Texas
    Mol Reprod Dev 81:183-93. 2014
    ..Interestingly, while resetting somatic transcription and associated epigenetic marks are absolutely required for development of SCNT embryos, life does not demand perfection...
  19. ncbi request reprint Early pregnancy diagnosis by transrectal ultrasonography in dairy cattle
    Juan E Romano
    Large Animal Clinical Sciences, College of Veterinary Medicine, Texas A and M University, College Station, TX 77845, USA
    Theriogenology 66:1034-41. 2006
    ..0 and 93.4% (P>0.05). In this study, heifers were diagnosed pregnant earlier than cows, and the maximum sensitivity and negative predictive value were obtained 3 days earlier in heifers than cows (days 26 and 29, respectively)...
  20. ncbi request reprint Influence of maturation culture period on the development of canine oocytes after in vitro maturation and fertilization
    Takeshige Otoi
    Department of Physiology and Pharmacology, College of Veterinary Medicine, Texas A and M University, College Station, TX 77843 4466, USA
    Reprod Nutr Dev 44:631-7. 2004
    ....
  21. ncbi request reprint Evidence for placental abnormality as the major cause of mortality in first-trimester somatic cell cloned bovine fetuses
    J R Hill
    Department of Veterinary Physiology and Pharmacology, Texas A and M University, College Station, Texas 77843 USA
    Biol Reprod 63:1787-94. 2000
    ..Our findings suggest that future research should focus on factors that promote placental and vascular growth and on fetomaternal interactions that promote placental attachment and villous formation...
  22. ncbi request reprint Analysis of DNA (cytosine 5) methyltransferase mRNA sequence and expression in bovine preimplantation embryos, fetal and adult tissues
    Michael C Golding
    Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine Texas A and M University, College Station, TX 77843 4466, USA
    Gene Expr Patterns 3:551-8. 2003
    ....
  23. ncbi request reprint Activation of equine nuclear transfer oocytes: methods and timing of treatment in relation to nuclear remodeling
    Young Ho Choi
    Departments of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Texas A and M University, College Station, Texas 77843 4466, USA
    Biol Reprod 70:46-53. 2004
    ..These results indicate that although nuclear remodeling occurs rapidly in equine cytoplasts, early activation does not improve embryonic development after reconstruction...
  24. ncbi request reprint New commercial opportunities for advanced reproductive technologies in horses, wildlife, and companion animals
    C R Long
    ViaGen Inc, 3312 Longmire Drive, College Station, TX 77845, USA
    Theriogenology 59:139-49. 2003
    ....
  25. pmc Suppression of prion protein in livestock by RNA interference
    Michael C Golding
    Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Howard Hughes Medical Institute, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Proc Natl Acad Sci U S A 103:5285-90. 2006
    ....

Research Grants2

  1. Production of New Models for Biomedical Research by RNAi
    Mark Westhusin; Fiscal Year: 2006
    ..Success of this project will lead to techniques which can be applied to a wide variety of different animal species, greatly expanding their usefulness as models for research involving human health and disease. ..
  2. Functional Analysis of the Embryonic Epigenome in a Non-rodent Model
    Mark E Westhusin; Fiscal Year: 2010
    ....