Ah Lim Tsai

Summary

Affiliation: Texas Medical Center
Country: USA

Publications

  1. ncbi request reprint Resonance Raman detection of the Fe-S bond in endothelial nitric oxide synthase
    Johannes P M Schelvis
    Department of Chemistry, New York University, New York, New York 10003, USA
    Biochemistry 41:5695-701. 2002
  2. ncbi request reprint Structural characterization of arachidonyl radicals formed by aspirin-treated prostaglandin H synthase-2
    Ah Lim Tsai
    Department of Internal Medicine, University of Texas Health Science Center at Houston, Houston, Texas 77030, USA
    J Biol Chem 277:38311-21. 2002
  3. ncbi request reprint Femtomolar sensitivity of a NO sensor from Clostridium botulinum
    Pierre Nioche
    Structural Biology Research Center and Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston, TX 77030, USA
    Science 306:1550-3. 2004
  4. ncbi request reprint Redox function of tetrahydrobiopterin and effect of L-arginine on oxygen binding in endothelial nitric oxide synthase
    Vladimir Berka
    Division of Hematology, Department of Internal Medicine, University of Texas Health Science Center, Houston, Texas 77030, USA
    Biochemistry 43:13137-48. 2004
  5. pmc Role of Tyr348 in Tyr385 radical dynamics and cyclooxygenase inhibitor interactions in prostaglandin H synthase-2
    Corina E Rogge
    Department of Internal Medicine, University of Texas Health Science Center, Houston, Texas 77030, USA
    Biochemistry 45:523-32. 2006
  6. pmc How do heme-protein sensors exclude oxygen? Lessons learned from cytochrome c', Nostoc puntiforme heme nitric oxide/oxygen-binding domain, and soluble guanylyl cyclase
    Ah Lim Tsai
    Division of Hematology, University of Texas Health Science Center at Houston, Houston, Texas 77225, USA
    Antioxid Redox Signal 17:1246-63. 2012
  7. pmc A "sliding scale rule" for selectivity among NO, CO, and O₂ by heme protein sensors
    Ah Lim Tsai
    Division of Hematology, Internal Medicine, University of Texas Medical School at Houston, Houston, Texas 77030, United States
    Biochemistry 51:172-86. 2012
  8. pmc Is Nostoc H-NOX a NO sensor or redox switch?
    Ah Lim Tsai
    Division of Hematology, Internal Medicine, University of Texas Houston Medical School, Houston, Texas 77030, USA
    Biochemistry 49:6587-99. 2010
  9. pmc Structural comparisons of arachidonic acid-induced radicals formed by prostaglandin H synthase-1 and -2
    Ah Lim Tsai
    Department of Internal Medicine, University of Texas Health Science Center at Houston, Houston, TX 77030, USA
    J Inorg Biochem 105:366-74. 2011
  10. pmc Dynamic ligand exchange in soluble guanylyl cyclase (sGC): implications for sGC regulation and desensitization
    Ah Lim Tsai
    Divisions of Hematology, University of Texas Health Science Center in Houston, Medical School, Houston, Texas 77030, USA
    J Biol Chem 286:43182-92. 2011

Collaborators

Detail Information

Publications54

  1. ncbi request reprint Resonance Raman detection of the Fe-S bond in endothelial nitric oxide synthase
    Johannes P M Schelvis
    Department of Chemistry, New York University, New York, New York 10003, USA
    Biochemistry 41:5695-701. 2002
    ..The implications of our findings for the function of eNOS will be discussed by comparison with P450(cam) and model compounds...
  2. ncbi request reprint Structural characterization of arachidonyl radicals formed by aspirin-treated prostaglandin H synthase-2
    Ah Lim Tsai
    Department of Internal Medicine, University of Texas Health Science Center at Houston, Houston, Texas 77030, USA
    J Biol Chem 277:38311-21. 2002
    ....
  3. ncbi request reprint Femtomolar sensitivity of a NO sensor from Clostridium botulinum
    Pierre Nioche
    Structural Biology Research Center and Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston, TX 77030, USA
    Science 306:1550-3. 2004
    ..Our findings have broad implications for understanding bacterial responses to NO, as well as for the activation of mammalian NO-sensitive guanylyl cyclase...
  4. ncbi request reprint Redox function of tetrahydrobiopterin and effect of L-arginine on oxygen binding in endothelial nitric oxide synthase
    Vladimir Berka
    Division of Hematology, Department of Internal Medicine, University of Texas Health Science Center, Houston, Texas 77030, USA
    Biochemistry 43:13137-48. 2004
    ....
  5. pmc Role of Tyr348 in Tyr385 radical dynamics and cyclooxygenase inhibitor interactions in prostaglandin H synthase-2
    Corina E Rogge
    Department of Internal Medicine, University of Texas Health Science Center, Houston, Texas 77030, USA
    Biochemistry 45:523-32. 2006
    ....
  6. pmc How do heme-protein sensors exclude oxygen? Lessons learned from cytochrome c', Nostoc puntiforme heme nitric oxide/oxygen-binding domain, and soluble guanylyl cyclase
    Ah Lim Tsai
    Division of Hematology, University of Texas Health Science Center at Houston, Houston, Texas 77225, USA
    Antioxid Redox Signal 17:1246-63. 2012
    ....
  7. pmc A "sliding scale rule" for selectivity among NO, CO, and O₂ by heme protein sensors
    Ah Lim Tsai
    Division of Hematology, Internal Medicine, University of Texas Medical School at Houston, Houston, Texas 77030, United States
    Biochemistry 51:172-86. 2012
    ..This multistep six-coordinate to five-coordinate mechanism appears to be common to all NO sensors that exclude O₂ binding to capture a lower level of cellular NO and prevent its consumption by dioxygenation...
  8. pmc Is Nostoc H-NOX a NO sensor or redox switch?
    Ah Lim Tsai
    Division of Hematology, Internal Medicine, University of Texas Houston Medical School, Houston, Texas 77030, USA
    Biochemistry 49:6587-99. 2010
    ..Such a large conformational transition suggests that Ns H-NOX could function as either a redox or a NO sensor in the cyanobacterium...
  9. pmc Structural comparisons of arachidonic acid-induced radicals formed by prostaglandin H synthase-1 and -2
    Ah Lim Tsai
    Department of Internal Medicine, University of Texas Health Science Center at Houston, Houston, TX 77030, USA
    J Inorg Biochem 105:366-74. 2011
    ..The improved understanding of the dynamics of AA and tyrosyl radicals in PGHS-1 and -2 will be useful for elucidating details of the cyclooxygenase mechanism, particularly the H-transfer between tyrosyl radical and AA...
  10. pmc Dynamic ligand exchange in soluble guanylyl cyclase (sGC): implications for sGC regulation and desensitization
    Ah Lim Tsai
    Divisions of Hematology, University of Texas Health Science Center in Houston, Medical School, Houston, Texas 77030, USA
    J Biol Chem 286:43182-92. 2011
    ..The implications of these observations for cellular NO/cGMP signaling and the process of rapid desensitization of sGC are discussed in the context of the proposed model of sGC/NO interactions and dynamic transformations...
  11. pmc Prostaglandin H synthase: resolved and unresolved mechanistic issues
    Ah Lim Tsai
    Department of Internal Medicine, University of Texas Health Science Center at Houston, Houston, Texas 77030, USA
    Arch Biochem Biophys 493:103-24. 2010
    ..The interconversions among radicals on Tyr385, on Tyr504, and on arachidonate, and their relationships to regulation and inactivation of the cyclooxygenase, are still under active investigation for both PGHS isozymes...
  12. ncbi request reprint Identification of Tyr504 as an alternative tyrosyl radical site in human prostaglandin H synthase-2
    Corina E Rogge
    Department of Internal Medicine, University of Texas Health Science Center at Houston, Houston, Texas 77030, USA
    Biochemistry 43:1560-8. 2004
    ....
  13. ncbi request reprint Three different oxygen-induced radical species in endothelial nitric-oxide synthase oxygenase domain under regulation by L-arginine and tetrahydrobiopterin
    Vladimir Berka
    Division of Hematology, Department of Internal Medicine, University of Texas Health Science Center at Houston, 77030, USA
    J Biol Chem 279:32243-51. 2004
    ..BH(4) prevented superoxide formation by chemical conversions of the Fe(II)O(2) intermediate, and l-arginine delayed superoxide formation by electronic interaction with the heme-bound oxygen...
  14. pmc Characterization of heme environment and mechanism of peroxide bond cleavage in human prostacyclin synthase
    Hui Chun Yeh
    Division of Hematology, Department of Internal Medicine, University of Texas Health Science Center, 6431 Fannin, Houston, TX 77030, USA
    Biochim Biophys Acta 1738:121-32. 2005
    ..PGIS catalyzes homolytic scission of the peroxide bond of a test substrate, 10-hydroperoxyoctadeca-8,12-dienoic acid, accompanied by formation of a heme intermediate with a Compound II-like optical spectrum...
  15. pmc Oxyferryl heme and not tyrosyl radical is the likely culprit in prostaglandin H synthase-1 peroxidase inactivation
    Gang Wu
    Department of Internal Medicine, University of Texas Health Science Center, Houston, Texas 77030, USA
    Biochemistry 46:534-42. 2007
    ..Computer modeling to a minimal mechanism was consistent with oxyferryl heme being the source of peroxidase inactivation...
  16. pmc Functional and structural roles of residues in the third extramembrane segment of adrenal cytochrome b561
    Wen Liu
    Department of Internal Medicine, University of Texas Health Science Center, Houston, Texas 77030, United States
    Biochemistry 50:3149-60. 2011
    ..These results also support our topological model of the adrenal cytochrome, which positions b(H) near the cytoplasmic side of the membrane...
  17. doi request reprint The selectivity of Vibrio cholerae H-NOX for gaseous ligands follows the "sliding scale rule" hypothesis. Ligand interactions with both ferrous and ferric Vc H-NOX
    Gang Wu
    Division of Hematology, Department of Internal Medicine, The University of Texas Medical School at Houston, 6431 Fannin Street, Houston, Texas 77030, United States
    Biochemistry 52:9432-46. 2013
    ..Characterization of both ferric and ferrous Vc H-NOX and their complexes with various ligands lays the foundation for understanding the possible dual roles in gas and redox sensing of Vc H-NOX...
  18. pmc Characterization of the heme environment in Arabidopsis thaliana fatty acid alpha-dioxygenase-1
    Wen Liu
    Department of Internal Medicine, University of Texas Health Science Center, Houston, Texas 77030, USA
    J Biol Chem 279:29805-15. 2004
    ..PADOX-1 is unusual in that it has a high affinity, inhibitory cyanide-binding site distinct from the distal heme face and the fatty acid site...
  19. pmc Axial ligation and stoichiometry of heme centers in adrenal cytochrome b561
    Yury Kamensky
    Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77251, USA
    Biochemistry 46:8647-58. 2007
    ..7. We provisionally assign the low-potential heme to the matrix side of the membrane; this arrangement suggests that the membrane potential modulates electron transport across the CG membrane...
  20. ncbi request reprint Redox properties of human endothelial nitric-oxide synthase oxygenase and reductase domains purified from yeast expression system
    Mei Du
    Division of Hematology, Department of Internal Medicine, University of Texas Health Science Center at Houston, Houston, Texas 77030, USA
    J Biol Chem 278:6002-11. 2003
    ....
  21. pmc Mechanism of binding of NO to soluble guanylyl cyclase: implication for the second NO binding to the heme proximal site
    Emil Martin
    Division of Cardiology, Internal Medicine, The University of Texas Medical School at Houston, Houston, Texas 77030, United States
    Biochemistry 51:2737-46. 2012
    ..Thus, as has been demonstrated with cytochrome c', a five-coordinate NO-sGC complex containing a proximal NO is formed after the binding of the second NO...
  22. pmc Cyclooxygenase reaction mechanism of PGHS--evidence for a reversible transition between a pentadienyl radical and a new tyrosyl radical by nitric oxide trapping
    Jian ming Lu
    Department of Internal Medicine, University of Texas Medical School at Houston, Houston, Texas 77030, USA
    J Inorg Biochem 105:356-65. 2011
    ..To reconcile the results from EPR and NO-trapping studies, we propose that the NS1c is in equilibrium with an AA pentadienyl radical, and that the latter reacts preferentially with NO...
  23. pmc Cyclooxygenase reaction mechanism of prostaglandin H synthase from deuterium kinetic isotope effects
    Gang Wu
    Department of Internal Medicine, University of Texas Health Science Center at Houston, Houston, TX 77030, USA
    J Inorg Biochem 105:382-90. 2011
    ..The large difference of the observed KIE from that of lipoxygenase indicates very different mechanism of hydrogen transfer...
  24. ncbi request reprint Soluble guanylyl cyclase: the nitric oxide receptor
    Emil Martin
    Department of Integrative Biology and Pharmacology, UT Health Science Center at Houston Medical School, Houston, TX 77030, USA
    Methods Enzymol 396:478-92. 2005
    ..Several approaches that enable characterization of the binding of NO to sGC heme moiety are also described...
  25. ncbi request reprint Cyclooxygenase inactivation kinetics during reaction of prostaglandin H synthase-1 with peroxide
    Gang Wu
    Division of Hematology, Department of Internal Medicine, University of Texas Health Science Center at Houston, Houston, Texas 77030, USA
    Biochemistry 42:13772-7. 2003
    ....
  26. ncbi request reprint Development of a bacterial system for high yield expression of fully functional adrenal cytochrome b561
    Wen Liu
    Department of Internal Medicine, University of Texas Health Science Center at Houston, 6431 Fannin Street, Houston, TX 77030, USA
    Protein Expr Purif 56:145-52. 2007
    ..The new bacterial expression system for cyt b561 offers a sixfold improvement in yield and other substantial advantages over existing insect and yeast cell systems for producing the recombinant cytochrome for structure-function studies...
  27. pmc Peroxide-induced radical formation at TYR385 and TYR504 in human PGHS-1
    Corina E Rogge
    Department of Internal Medicine, University of Texas Health Science Center at Houston, Houston, TX 77030, USA
    J Inorg Biochem 103:912-22. 2009
    ..Y504F mutants in both hPGHS-1 and -2 significantly decreased the cyclooxygenase activation efficiency, indicating that formation of the Tyr504 radical is functionally important for both isoforms...
  28. pmc Spectroscopic characterization of the oxyferrous complex of prostacyclin synthase in solution and in trapped sol-gel matrix
    Hui Chun Yeh
    Division of Hematology, Department of Internal Medicine, University of Texas Health Science Center, Houston, TX 77030, USA
    FEBS J 275:2305-14. 2008
    ..This result suggests that the sol-gel method may be applied to other P450s to generate a stable intermediate in the di-oxygen activation...
  29. ncbi request reprint Oxygen-induced radical intermediates in the nNOS oxygenase domain regulated by L-arginine, tetrahydrobiopterin, and thiol
    Vladimir Berka
    Division of Hematology, Department of Internal Medicine, University of Texas Health Science Center, Houston, Texas 77030, USA
    Biochemistry 47:405-20. 2008
    ..This new role of thiol found only for nNOS may be significant in neurodegenerative diseases...
  30. pmc His92 and His110 selectively affect different heme centers of adrenal cytochrome b(561)
    Wen Liu
    Department of Internal Medicine, University of Texas Health Science Center at Houston, Houston, Texas, USA
    Biochim Biophys Acta 1777:1218-28. 2008
    ..These results indicate that His92 can be considered part of the b(H) heme center, and His110 part of the b(L) heme center, in adrenal cyt b(561)...
  31. pmc Cyclooxygenase competitive inhibitors alter tyrosyl radical dynamics in prostaglandin H synthase-2
    Gang Wu
    Department of Internal Medicine, University of Texas Health Science Center at Houston, Houston, Texas 77030, USA
    Biochemistry 48:11902-11. 2009
    ..This dramatic modulation of tyrosyl radical stability by cyclooxygenase site ligands suggests a mechanism for regulating the reactivity of PGHS tyrosyl radicals with cellular antioxidants...
  32. pmc Binding kinetics of calmodulin with target peptides of three nitric oxide synthase isozymes
    Gang Wu
    Department of Internal Medicine, University of Texas Health Science Center at Houston, Houston, Texas 77030, USA
    J Inorg Biochem 105:1226-37. 2011
    ..Only iNOS(507-531) was able to bind apo Alexa-CaM, but in a very different conformation from its binding to holo Alexa-CaM...
  33. pmc High-yield production, purification and characterization of functional human duodenal cytochrome b in an Escherichia coli system
    Wen Liu
    Department of Internal Medicine, University of Texas Health Science Center, Houston, TX 77030, United States
    Protein Expr Purif 79:115-21. 2011
    ....
  34. pmc Characterization of the peroxidase mechanism upon reaction of prostacyclin synthase with peracetic acid. Identification of a tyrosyl radical intermediate
    Hui Chun Yeh
    Division of Hematology, Department of Internal Medicine, University of Texas Health Science Center at Houston, 6431 Fannin, Houston, Texas 77030, USA
    Biochemistry 48:917-28. 2009
    ..The inactivation, however, was prevented by the exogenous reductant guaiacol. The studies of PGIS with PA described herein provide a mechanistic model of a peroxidase reaction catalyzed by the class III cytochromes P450...
  35. ncbi request reprint Redox properties of human hemoglobin in complex with fractionated dimeric and polymeric human haptoglobin
    Todd L Mollan
    Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20852, USA
    Free Radic Biol Med 69:265-77. 2014
    ....
  36. ncbi request reprint Comparison of the properties of prostaglandin H synthase-1 and -2
    Richard J Kulmacz
    Department of Internal Medicine, University of Texas Health Science Center at Houston, Houston, TX 77030, USA
    Prog Lipid Res 42:377-404. 2003
    ..The focus of this review is on the structural and biochemical distinctions between PGHS-1 and-2, and how these differences relate to the functional divergence between the two isoforms...
  37. pmc Reaction mechanisms of 15-hydroperoxyeicosatetraenoic acid catalyzed by human prostacyclin and thromboxane synthases
    Hui Chun Yeh
    Division of Hematology, Department of Internal Medicine, University of Texas Health Science Center, 6431 Fannin, Houston, TX 77030, USA
    Arch Biochem Biophys 461:159-68. 2007
    ..Similar K(M) values for homolytic and heterolytic cleavages were found for PGIS ( approximately 60 microM 15-HPETE) and TXAS ( approximately 80 microM 15-HPETE), making PGIS a more efficient catalyst for the 15-HPETE reaction...
  38. pmc Peroxynitrite induces destruction of the tetrahydrobiopterin and heme in endothelial nitric oxide synthase: transition from reversible to irreversible enzyme inhibition
    Weiguo Chen
    Davis Heart and Lung Research Institute, Division of Cardiovascular Medicine, Department of Internal Medicine, College of Medicine, The Ohio State University, Columbus, Ohio 43210, USA
    Biochemistry 49:3129-37. 2010
    ..Therefore, ONOO(-)-induced eNOS inactivation is primarily due to oxidation of BH(4) and irreversible destruction of the heme/heme center...
  39. ncbi request reprint Cobalt- and nickel-binding property of cullin-2
    Koichi Kanaya
    Department of Cardiology, University of Texas M D Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA
    Biochem Biophys Res Commun 290:294-9. 2002
    ..Interestingly, Co(2+) did not affect the complex formation of the ligase, suggesting that the metal binding to cullin-2 affects the function, but not the assembly of the E3-ligase...
  40. ncbi request reprint No scavenging and the hypertensive effect of hemoglobin-based blood substitutes
    John S Olson
    Department of Biochemistry and Cell Biology, W M Keck Center for Computational Biology, Rice University, Houston, TX 77005, USA
    Free Radic Biol Med 36:685-97. 2004
    ....
  41. pmc α-Hemoglobin stabilizing protein (AHSP) markedly decreases the redox potential and reactivity of α-subunits of human HbA with hydrogen peroxide
    Todd L Mollan
    Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20852, USA
    J Biol Chem 288:4288-98. 2013
    ..Hexacoordination in the AHSP·met-α complex markedly decreases the rate of the initial H(2)O(2) reaction with iron and thus provides α-subunits protection against damaging oxidative reactions...
  42. doi request reprint Enhancement of the electron spin resonance of single-walled carbon nanotubes by oxygen removal
    William D Rice
    Department of Electrical and Computer Engineering, Rice University, Houston, Texas 77005, USA
    ACS Nano 6:2165-73. 2012
    ..We hypothesize that physisorbed molecular oxygen acts as an acceptor (p-type), compensating the donor-like (n-type) defects that are responsible for the ESR signal in bulk SWCNTs...
  43. ncbi request reprint Ligand selectivity of soluble guanylyl cyclase: effect of the hydrogen-bonding tyrosine in the distal heme pocket on binding of oxygen, nitric oxide, and carbon monoxide
    Emil Martin
    Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center, USA
    J Biol Chem 281:27836-45. 2006
    ..We propose that the mechanism of oxygen exclusion by sGC not only involves the lack of hydrogen bonding in the distal heme pocket, but also depends on structural elements from other domains of sGC...
  44. ncbi request reprint Insertion of an N7-methylguanine mRNA cap between two coplanar aromatic residues of a cap-binding protein is fast and selective for a positively charged cap
    Guanghui Hu
    Graduate Program in Structural and Computational Biology and Molecular Biophysics, Howard Hughes Medical Institute and Mars and Verna McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine Houston, Texas 77030, USA
    J Biol Chem 278:51515-20. 2003
    ....
  45. pmc HAMP domain signal relay mechanism in a sensory rhodopsin-transducer complex
    Jihong Wang
    Center for Membrane Biology, Department of Biochemistry and Molecular Biology, Houston, Texas 77030, USA
    J Biol Chem 287:21316-25. 2012
    ....
  46. pmc Different dark conformations function in color-sensitive photosignaling by the sensory rhodopsin I-HtrI complex
    Jun Sasaki
    Center for Membrane Biology, Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston, Texas 77030, USA
    Biophys J 92:4045-53. 2007
    ....
  47. pmc Opposite displacement of helix F in attractant and repellent signaling by sensory rhodopsin-Htr complexes
    Jun Sasaki
    Center for Membrane Biology, Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston, Texas 77030, USA
    J Biol Chem 286:18868-77. 2011
    ....
  48. ncbi request reprint The cyclooxygenase reaction mechanism
    Wilfred A van der Donk
    Department of Chemistry, University of Illinois at Urbana Champaign, 600 South Mathews Ave, Urbana, Illinois 61801, USA
    Biochemistry 41:15451-8. 2002
  49. pmc Determination of the structural environment of the tyrosyl radical in prostaglandin H2 synthase-1: a high frequency ENDOR/EPR study
    John C Wilson
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Am Chem Soc 127:1618-9. 2005
    ..Possible hydrogen-bonding partners, as well as implications regarding the mechanism of self-inactivation for PGHS, are discussed...
  50. ncbi request reprint Nitric oxide (NO) traffic in endothelial NO synthase. Evidence for a new NO binding site dependent on tetrahydrobiopterin?
    Anny Slama-Schwok
    Laboratory for Optics and Biosciences, INSERM U451, CNRS Unite Mixte de Recherche 7645, Ecole Polytechnique, 91128 Palaiseau, France
    J Biol Chem 277:7581-6. 2002
    ..The new non-heme site may act as a "doorstep" to the heme pocket and control NO escape from eNOS...
  51. ncbi request reprint High-field EPR study of tyrosyl radicals in prostaglandin H(2) synthase-1
    Pierre Dorlet
    Section de Bioenergetique, CNRS URA 2096, CEA Saclay, Bat 532, 91191 Gif sur Yvette Cedex, France
    Biochemistry 41:6107-14. 2002
    ..The 130 GHz pulsed EPR experiments performed on the wide-doublet species indicated that the possible interaction between the radical and the oxoferryl heme species was very weak...
  52. ncbi request reprint Synthesis of isotopically labeled arachidonic acids to probe the reaction mechanism of prostaglandin H synthase
    Sheng Peng
    Department of Chemistry, University of Illinois at Urbana Champaign, 600 South Mathews Avenue, Urbana, IL 61801, USA
    J Am Chem Soc 124:10785-96. 2002
    ..Use of 16-[(2)H(2)]-arachidonic acid indicated that only one of the protons at C16 gives rise to a strong hyperfine interaction. The findings are discussed in the context of two proposed mechanisms for the cyclooxygenase reaction...
  53. ncbi request reprint Evidence for the pathophysiological role of endogenous methylarginines in regulation of endothelial NO production and vascular function
    Arturo J Cardounel
    Department of Pharmacology, Davis Heart and Lung Research Institute, The Ohio State University College of Medicine, Columbus, Ohio 43210, USA
    J Biol Chem 282:879-87. 2007
    ..Thus, MAs are critical mediators of vascular dysfunction following vascular injury...
  54. ncbi request reprint Synchronous photoinitiation of endothelial NO synthase activity by a nanotrigger targeted at its NADPH site
    Edward Beaumont
    Unité 696, INSERM, Palaiseau France
    J Am Chem Soc 129:2178-86. 2007
    ..The selective targeting of the nanotrigger to NADPH sites should allow controlled initiation of the enzymatic activity of numerous proteins containing an NADPH site...

Research Grants26

  1. STRUCTURE/FUNCTION AND REACTION MECHANISM OF PGHS
    Ah Lim Tsai; Fiscal Year: 2002
    ..Understanding the basic reaction mechanism of PGHS in the cardiovascular systems should provide great insight into the control and regulation of the physiological and pathophysiological events associated with prostaglandins. ..
  2. STRUCTURE/FUNCTION AND REACTION MECHANISM OF PGHS
    Ah Lim Tsai; Fiscal Year: 2002
    ..Understanding the basic reaction mechanism of PGHS in the cardiovascular systems should provide great insight into the control and regulation of the physiological and pathophysiological events associated with prostaglandins. ..
  3. Structure-Function and Reacation Mechanism of eNOS
    Ah Lim Tsai; Fiscal Year: 2004
    ..The last Aim will provide understanding of the role of reductase and CaM in regulating the redox coupling with the oxygenase domain thus lead to an elucidation of the reaction of the reaction mechanism of the whole eNOS. ..
  4. Structure-Function and Reacation Mechanism of eNOS
    Ah Lim Tsai; Fiscal Year: 2005
    ..The last Aim will provide understanding of the role of reductase and CaM in regulating the redox coupling with the oxygenase domain thus lead to an elucidation of the reaction of the reaction mechanism of the whole eNOS. ..
  5. STRUCTURE/FUNCTION AND REACTION MECHANISM OF PGHS
    Ah Lim Tsai; Fiscal Year: 2005
    ..The 2nd Aim will address the role of tyrosyl radical dynamics on PGHS catalysis. The last aim will provide direct data on the membrane topology of PGHS and the mechanism of its substrate presentation and product release. ..
  6. Structure-Function and Reacation Mechanism of eNOS
    Ah Lim Tsai; Fiscal Year: 2006
    ..The last Aim will provide understanding of the role of reductase and CaM in regulating the redox coupling with the oxygenase domain thus lead to an elucidation of the reaction of the reaction mechanism of the whole eNOS. ..
  7. STRUCTURE/FUNCTION AND REACTION MECHANISM OF PGHS
    Ah Lim Tsai; Fiscal Year: 2006
    ..The 2nd Aim will address the role of tyrosyl radical dynamics on PGHS catalysis. The last aim will provide direct data on the membrane topology of PGHS and the mechanism of its substrate presentation and product release. ..
  8. STRUCTURE/FUNCTION AND REACTION MECHANISM OF PGHS
    Ah Lim Tsai; Fiscal Year: 2007
    ..The 2nd Aim will address the role of tyrosyl radical dynamics on PGHS catalysis. The last aim will provide direct data on the membrane topology of PGHS and the mechanism of its substrate presentation and product release. ..
  9. Structure-Function and Reacation Mechanism of eNOS
    Ah Lim Tsai; Fiscal Year: 2007
    ..The last Aim will provide understanding of the role of reductase and CaM in regulating the redox coupling with the oxygenase domain thus lead to an elucidation of the reaction of the reaction mechanism of the whole eNOS. ..
  10. STRUCTURE /FUNCTION AND REACTION MECHANISM OF NITRIC OXI
    Ah Lim Tsai; Fiscal Year: 2002
    ..abstract_text> ..
  11. STRUCTURE /FUNCTION AND REACTION MECHANISM OF NITRIC OXI
    Ah Lim Tsai; Fiscal Year: 2001
    ..abstract_text> ..
  12. STRUCTURE /FUNCTION AND REACTION MECHANISM OF NITRIC OXI
    Ah Lim Tsai; Fiscal Year: 1999
    ..abstract_text> ..
  13. STRUCTURE/FUNCTION AND REACTION MECHANISM OF PGHS
    Ah Lim Tsai; Fiscal Year: 1999
    ..Understanding the basic reaction mechanism of PGHS in the cardiovascular systems should provide great insight into the control and regulation of the physiological and pathophysiological events associated with prostaglandins. ..
  14. PROSTAGLANDIN H SYNTHASE--REACTION MECHANISM
    Ah Lim Tsai; Fiscal Year: 1992
    ..The third and final approach of site-directed mutagenesis will help to pinpoint the important amino acid residues and Will enhance the elucidation of the overall enzymic reaction mechanism...
  15. PROSTAGLANDIN H SYNTHASE--REACTION MECHANISM
    Ah Lim Tsai; Fiscal Year: 1993
    ..The third and final approach of site-directed mutagenesis will help to pinpoint the important amino acid residues and Will enhance the elucidation of the overall enzymic reaction mechanism...
  16. STRUCTURE/FUNCTION AND REACTION MECHANISM OF PGHS
    Ah Lim Tsai; Fiscal Year: 2000
    ..Understanding the basic reaction mechanism of PGHS in the cardiovascular systems should provide great insight into the control and regulation of the physiological and pathophysiological events associated with prostaglandins. ..
  17. STRUCTURE /FUNCTION AND REACTION MECHANISM OF NITRIC OXI
    Ah Lim Tsai; Fiscal Year: 2000
    ..abstract_text> ..
  18. STRUCTURE/FUNCTION AND REACTION MECHANISM OF PGHS
    Ah Lim Tsai; Fiscal Year: 2001
    ..Understanding the basic reaction mechanism of PGHS in the cardiovascular systems should provide great insight into the control and regulation of the physiological and pathophysiological events associated with prostaglandins. ..
  19. Radical Intermediates of Nitric Oxide Synthase & Myocardial Ischemia Reperfusion
    Ah Lim Tsai; Fiscal Year: 2010
    ..The regulation of these radical intermediates by substrate, cofactors and thiol also are studied, both in vitro and ex vivo, in order to elucidate the underlying disease mechanism of myocardial ischemia and reperfusion injury. ..