DOUGLAS STOCCO

Summary

Affiliation: Texas Tech University Health Sciences Center
Country: USA

Publications

  1. ncbi Multiple signaling pathways regulating steroidogenesis and steroidogenic acute regulatory protein expression: more complicated than we thought
    Douglas M Stocco
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    Mol Endocrinol 19:2647-59. 2005
  2. pmc Steroidogenic acute regulatory protein expression in the central nervous system
    Steven R King
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center Lubbock, TX, USA
    Front Endocrinol (Lausanne) 2:72. 2011
  3. pmc The role of specific mitogen-activated protein kinase signaling cascades in the regulation of steroidogenesis
    Pulak R Manna
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA
    J Signal Transduct 2011:821615. 2011
  4. pmc Short RNA molecules with high binding affinity to the KH motif of A-kinase anchoring protein 1 (AKAP1): implications for the regulation of steroidogenesis
    Petar N Grozdanov
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    Mol Endocrinol 26:2104-17. 2012
  5. doi The role of JUN in the regulation of PRKCC-mediated STAR expression and steroidogenesis in mouse Leydig cells
    Pulak R Manna
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    J Mol Endocrinol 41:329-41. 2008
  6. ncbi Involvement of protein kinase C and cyclic adenosine 3',5'-monophosphate-dependent kinase in steroidogenic acute regulatory protein expression and steroid biosynthesis in Leydig cells
    Youngah Jo
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    Biol Reprod 73:244-55. 2005
  7. ncbi Differential regulation of steroid hormone biosynthesis in R2C and MA-10 Leydig tumor cells: role of SR-B1-mediated selective cholesteryl ester transport
    Rekha M Rao
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    Biol Reprod 68:114-21. 2003
  8. ncbi Regulation of Leydig cell steroidogenesis by extracellular signal-regulated kinase 1/2: role of protein kinase A and protein kinase C signaling
    Pulak R Manna
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    J Endocrinol 193:53-63. 2007
  9. pmc Blocking L-type calcium channels reduced the threshold of cAMP-induced steroidogenic acute regulatory gene expression in MA-10 mouse Leydig cells
    Akhilesh K Pandey
    Garrison Institute on Aging, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    J Endocrinol 204:67-74. 2010
  10. ncbi Molecular mechanisms of insulin-like growth factor-I mediated regulation of the steroidogenic acute regulatory protein in mouse leydig cells
    Pulak R Manna
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    Mol Endocrinol 20:362-78. 2006

Research Grants

Collaborators

Detail Information

Publications45

  1. ncbi Multiple signaling pathways regulating steroidogenesis and steroidogenic acute regulatory protein expression: more complicated than we thought
    Douglas M Stocco
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    Mol Endocrinol 19:2647-59. 2005
    ....
  2. pmc Steroidogenic acute regulatory protein expression in the central nervous system
    Steven R King
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center Lubbock, TX, USA
    Front Endocrinol (Lausanne) 2:72. 2011
    ..The identification of the steroidogenic acute regulatory protein in steroid-producing neural cells provides a new tool to understand the sites, regulation, and importance of their synthesis...
  3. pmc The role of specific mitogen-activated protein kinase signaling cascades in the regulation of steroidogenesis
    Pulak R Manna
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA
    J Signal Transduct 2011:821615. 2011
    ..This paper summarizes the current level of understanding concerning the roles of the MAPK signaling cascades in the regulation of StAR expression and steroidogenesis in different steroidogenic cell models...
  4. pmc Short RNA molecules with high binding affinity to the KH motif of A-kinase anchoring protein 1 (AKAP1): implications for the regulation of steroidogenesis
    Petar N Grozdanov
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    Mol Endocrinol 26:2104-17. 2012
    ....
  5. doi The role of JUN in the regulation of PRKCC-mediated STAR expression and steroidogenesis in mouse Leydig cells
    Pulak R Manna
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    J Mol Endocrinol 41:329-41. 2008
    ..These findings describe the mechanisms by which JUN influences PRKCC signaling and provide additional and novel insight into the regulation of the steroidogenic machinery in mouse Leydig cells...
  6. ncbi Involvement of protein kinase C and cyclic adenosine 3',5'-monophosphate-dependent kinase in steroidogenic acute regulatory protein expression and steroid biosynthesis in Leydig cells
    Youngah Jo
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    Biol Reprod 73:244-55. 2005
    ..This study also demonstrates for the first time a role for PMA-induced PKC in StAR protein regulation and the requirement for submaximal doses of cAMP to produce steroids in Leydig cells...
  7. ncbi Differential regulation of steroid hormone biosynthesis in R2C and MA-10 Leydig tumor cells: role of SR-B1-mediated selective cholesteryl ester transport
    Rekha M Rao
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    Biol Reprod 68:114-21. 2003
    ....
  8. ncbi Regulation of Leydig cell steroidogenesis by extracellular signal-regulated kinase 1/2: role of protein kinase A and protein kinase C signaling
    Pulak R Manna
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    J Endocrinol 193:53-63. 2007
    ..These findings clearly demonstrate that the ERK1/2 signaling cascade involved in regulating StAR expression and steroid synthesis is mediated by multiple factors and pathways and is stimulus specific in mouse Leydig cells...
  9. pmc Blocking L-type calcium channels reduced the threshold of cAMP-induced steroidogenic acute regulatory gene expression in MA-10 mouse Leydig cells
    Akhilesh K Pandey
    Garrison Institute on Aging, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    J Endocrinol 204:67-74. 2010
    ..Blocking L-type Ca(2+) channel attenuated the inhibition and reduced the threshold of cAMP-induced Star gene expression in Leydig cells...
  10. ncbi Molecular mechanisms of insulin-like growth factor-I mediated regulation of the steroidogenic acute regulatory protein in mouse leydig cells
    Pulak R Manna
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    Mol Endocrinol 20:362-78. 2006
    ..Collectively, the present studies identify molecular events in IGF-I signaling that may influence testicular growth, development, and the Leydig cell steroidogenic machinery through autocrine/paracrine regulation...
  11. ncbi Assessment of the role of activator protein-1 on transcription of the mouse steroidogenic acute regulatory protein gene
    Pulak R Manna
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    Mol Endocrinol 18:558-73. 2004
    ..These findings demonstrate that Fos and Jun can bind to the TGACTGA element in the StAR promoter and provide novel insights into the mechanisms regulating StAR gene transcription...
  12. ncbi Involvement of peroxisome proliferator-activated receptor gamma in gonadal steroidogenesis and steroidogenic acute regulatory protein expression
    Mariusz P Kowalewski
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX, USA
    Reprod Fertil Dev 21:909-22. 2009
    ..Ciglitazone-activated PPARgamma appears to increase the sensitivity of Leydig and granulosa cells to cAMP stimulation, possibly via upregulation of c-JUN expression...
  13. ncbi Chrysin, a natural flavonoid enhances steroidogenesis and steroidogenic acute regulatory protein gene expression in mouse Leydig cells
    Kuladip Jana
    Garrison Institute on Aging, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    J Endocrinol 197:315-23. 2008
    ..These results suggest that while chrysin itself is unable to induce StAR gene expression and steroidogenesis, it appears to function by increasing the sensitivity of Leydig cells to cAMP stimulation...
  14. pmc Role of dosage-sensitive sex reversal, adrenal hypoplasia congenita, critical region on the X chromosome, gene 1 in protein kinase A- and protein kinase C-mediated regulation of the steroidogenic acute regulatory protein expression in mouse Leydig tumor c
    Pulak R Manna
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    Endocrinology 150:187-99. 2009
    ..These findings provide insight into the molecular events by which DAX-1 influences the PKA and PKC signaling pathways involved in the regulation of the StAR protein and steroidogenesis in mouse Leydig tumor cells...
  15. ncbi Mechanisms of epidermal growth factor signaling: regulation of steroid biosynthesis and the steroidogenic acute regulatory protein in mouse Leydig tumor cells
    Pulak R Manna
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    Biol Reprod 67:1393-404. 2002
    ..These findings provide novel insights into the mEGF-induced regulatory cascades associated with steroid synthesis and StAR protein expression in mouse Leydig cells...
  16. ncbi Regulation of steroidogenesis and steroidogenic acute regulatory protein in R2C cells by DAX-1 (dosage-sensitive sex reversal, adrenal hypoplasia congenita, critical region on the X chromosome, gene-1)
    Youngah Jo
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    Endocrinology 145:5629-37. 2004
    ....
  17. pmc Mechanisms of protein kinase C signaling in the modulation of 3',5'-cyclic adenosine monophosphate-mediated steroidogenesis in mouse gonadal cells
    Pulak R Manna
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    Endocrinology 150:3308-17. 2009
    ..Collectively, these findings provide insight into the mechanisms by which PKC modulates cAMP/protein kinase A responsiveness involved in regulating the steroidogenic response in mouse gonadal cells...
  18. ncbi cAMP-independent signaling regulates steroidogenesis in mouse Leydig cells in the absence of StAR phosphorylation
    Pulak R Manna
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA
    J Mol Endocrinol 37:81-95. 2006
    ..Collectively, these findings demonstrate that, in mouse Leydig cells, cAMP-independent signaling events regulate steroidogenesis in a StAR phosphorylation-independent manner...
  19. pmc The differential regulation of steroidogenic acute regulatory protein-mediated steroidogenesis by type I and type II PKA in MA-10 cells
    Matthew T Dyson
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, 3601 4th Street, Lubbock, TX 79430, United States
    Mol Cell Endocrinol 300:94-103. 2009
    ..These experiments delineate the discrete effects that type I and type II PKA exert on STAR-mediated steroidogenesis, and suggest complimentary roles for each subtype in coordinating steroidogenesis...
  20. ncbi Regulation of steroidogenesis and the steroidogenic acute regulatory protein by a member of the cAMP response-element binding protein family
    Pulak R Manna
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    Mol Endocrinol 16:184-99. 2002
    ..These are the first observations to provide evidence for the functional involvement of a CREB/CREM family member in the acute regulation of trophic hormone-stimulated steroidogenesis and StAR gene expression...
  21. ncbi Mitochondrial A-kinase anchoring protein 121 binds type II protein kinase A and enhances steroidogenic acute regulatory protein-mediated steroidogenesis in MA-10 mouse leydig tumor cells
    Matthew T Dyson
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    Biol Reprod 78:267-77. 2008
    ..This suggests that AKAP121 and PKAR2A serve to enhance steroidogenesis by directing the synthesis and activation of STAR at the mitochondria in response to cAMP...
  22. ncbi Cyclooxygenase-2 regulation of the age-related decline in testosterone biosynthesis
    XingJia Wang
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    Endocrinology 146:4202-8. 2005
    ..The present study suggests a novel mechanism in male aging involving COX2 and a potential application of the mechanism to delay the age-related decline in testosterone biosynthesis...
  23. pmc Involvement of the thromboxane A2 receptor in the regulation of steroidogenic acute regulatory gene expression in murine Leydig cells
    Akhilesh K Pandey
    Garrison Institute on Aging, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    Endocrinology 150:3267-73. 2009
    ....
  24. ncbi The involvement of epoxygenase metabolites of arachidonic acid in cAMP-stimulated steroidogenesis and steroidogenic acute regulatory protein gene expression
    XingJia Wang
    Department of Neuropsychiatry, Garrison Institute on Aging, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    J Endocrinol 190:871-8. 2006
    ..This study suggests that cAMP stimulation of MA-10 cells increases epoxygenase-generated AA metabolites and the co-action of these metabolites with PKA significantly increases StAR gene expression and steroid hormone production...
  25. ncbi Inhibition of cyclooxygenase-2 activity enhances steroidogenesis and steroidogenic acute regulatory gene expression in MA-10 mouse Leydig cells
    XingJia Wang
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    Endocrinology 144:3368-75. 2003
    ..The results of the present study suggest that inhibition of COX2 activity increases the sensitivity of steroidogenesis to cAMP stimulation in MA-10 Leydig cells...
  26. ncbi Involvement of 5-lipoxygenase metabolites of arachidonic acid in cyclic AMP-stimulated steroidogenesis and steroidogenic acute regulatory protein gene expression
    Xing Jia Wang
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA
    J Steroid Biochem Mol Biol 85:159-66. 2003
    ..The results further suggested that the AA-responsive element resides in the -67/-96 region of the StAR promoter...
  27. ncbi Interaction between arachidonic acid and cAMP signaling pathways enhances steroidogenesis and StAR gene expression in MA-10 Leydig tumor cells
    Xing Jia Wang
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA
    Mol Cell Endocrinol 188:55-63. 2002
    ..The results from this study demonstrate that AA and cAMP act in a highly synergistic manner to increase the sensitivity of steroid production to trophic hormone stimulation and probably do so by increasing StAR gene expression...
  28. ncbi The decline in testosterone biosynthesis during male aging: a consequence of multiple alterations
    XingJia Wang
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA
    Mol Cell Endocrinol 238:1-7. 2005
    ....
  29. ncbi Clinical disorders associated with abnormal cholesterol transport: mutations in the steroidogenic acute regulatory protein
    Douglas M Stocco
    Texas Technological University Health Sciences Center, 3601 4th Street, Department of Cell Biology and Biochemistry, Lubbock, TX 79430, USA
    Mol Cell Endocrinol 191:19-25. 2002
    ....
  30. pmc Inhibition of thromboxane a synthase activity enhances steroidogenesis and steroidogenic acute regulatory gene expression in MA-10 mouse Leydig cells
    XingJia Wang
    Garrison Institute on Aging, Department of Neuropsychiatry, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA
    Endocrinology 149:851-7. 2008
    ....
  31. ncbi Regulation of steroid hormone biosynthesis in R2C and MA-10 Leydig tumor cells: role of the cholesterol transfer proteins StAR and PBR
    Rekha M Rao
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA
    Endocr Res 28:387-94. 2002
    ..We also determined that the affinity of ligand binding to the PBR is comparable in the two cell lines, suggesting that PBR is unlikely to be solely responsible for the constitutive nature of R2C cell steroidogenesis...
  32. ncbi Crosstalk of CREB and Fos/Jun on a single cis-element: transcriptional repression of the steroidogenic acute regulatory protein gene
    Pulak R Manna
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    J Mol Endocrinol 39:261-77. 2007
    ....
  33. ncbi Involvement of multiple transcription factors in the regulation of steroidogenic acute regulatory protein gene expression
    Pulak R Manna
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA
    Steroids 68:1125-34. 2003
    ..Nevertheless, it has become apparent that functional cooperation, interaction, and alteration of different transcription factors are involved in the fine-tuning of the regulatory events associated with StAR gene transcription...
  34. ncbi Differences in steroidogenic acute regulatory protein expression from the luteinizing-granulosa cells of patients undergoing in vitro fertilization with embryo transfer: implications for cycle outcomes
    Jennifer Phy
    Department of Obstetrics and Gynecology, Texas Tech University Health Sciences Center, Lubbock 79430, USA
    Am J Obstet Gynecol 186:872-5. 2002
    ..The objective of the present study was to determine whether StAR expression differed in normal responders and poor responders undergoing IVF-ET...
  35. pmc Regulation of the steroidogenic acute regulatory protein gene expression: present and future perspectives
    Pulak R Manna
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA
    Mol Hum Reprod 15:321-33. 2009
    ....
  36. pmc The involvement of specific PKC isoenzymes in phorbol ester-mediated regulation of steroidogenic acute regulatory protein expression and steroid synthesis in mouse Leydig cells
    Pulak R Manna
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    Endocrinology 152:313-25. 2011
    ....
  37. ncbi Role of basic leucine zipper proteins in transcriptional regulation of the steroidogenic acute regulatory protein gene
    Pulak R Manna
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA
    Mol Cell Endocrinol 302:1-11. 2009
    ..This review will focus on the current level of understanding of the roles of selective bZIP family proteins within the complex series of processes involved in regulating StAR gene transcription...
  38. ncbi An essential component in steroid synthesis, the steroidogenic acute regulatory protein, is expressed in discrete regions of the brain
    Steven R King
    Scott Department of Urology, Baylor College of Medicine, Houston, Texas 77030 3498, USA
    J Neurosci 22:10613-20. 2002
    ..These data support a role for StAR in the production of neurosteroids and identify potential sites of active de novo steroid synthesis in the brain...
  39. ncbi The life cycle of the steroidogenic acute regulatory (StAR) protein: from transcription through proteolysis
    Zvi Granot
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
    Endocr Res 28:375-86. 2002
    ..Collectively, this study defines StAR as a unique protein that can authentically be used to probe multiple proteolytic activities in mammalian mitochondria...
  40. ncbi Stimulatory effect of progesterone on the expression of steroidogenic acute regulatory protein in MA-10 Leydig cells
    Heidi Schwarzenbach
    Institute for Hormone and Fertility Research, University of Hamburg, D 22529 Hamburg, Germany
    Biol Reprod 68:1054-63. 2003
    ..Furthermore, these results provide a new insight, indicating that progesterone mediates the activation of StAR expression exerted presumably through a novel, nonclassical progesterone receptor in mouse Leydig cells...
  41. ncbi Steroidogenic acute regulatory protein in ovarian follicles of gonadotropin-stimulated rats is regulated by a gonadotropin-releasing hormone agonist
    Griselda Irusta
    Instituto de Biologia y Medicina Experimental IByME CONICET, Facultad de Ciencias Exactas, Universidad de Buenos Aires y Nacional de la Plata, Argentina
    Biol Reprod 68:1577-83. 2003
    ..Collectively, these findings demonstrate that the inhibitory effect of LA on ovarian androgen and estradiol levels is related to changes in the follicular levels of StAR protein and steroid production...
  42. ncbi Effects of genistein, resveratrol, and quercetin on steroidogenesis and proliferation of MA-10 mouse Leydig tumor cells
    Yu Chyu Chen
    Medical and Research Services, WJB Dorn Veterans Medical Center, Columbia, South Carolina 29201, USA
    J Endocrinol 192:527-37. 2007
    ..Taken together, these results indicate that phytoestrogens have differential effects on steroidogenesis in MA-10 cells...
  43. ncbi Proteolysis of normal and mutated steroidogenic acute regulatory proteins in the mitochondria: the fate of unwanted proteins
    Zvi Granot
    Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
    Mol Endocrinol 17:2461-76. 2003
    ..Collectively, this study defines StAR as the first naturally occurring mitochondrial protein that can serve as a substrate to probe multiple proteolytic activities in mammalian mitochondria...
  44. ncbi The modulatory role of transforming growth factor beta1 and androstenedione on follicle-stimulating hormone-induced gelatinase secretion and steroidogenesis in rat granulosa cells
    Ferng Chun Ke
    Institute of Molecular and Cellular Biology, National Taiwan University, Taipei 106, Taiwan
    Biol Reprod 70:1292-8. 2004
    ....
  45. ncbi Lindane, a gap junction blocker, suppresses FSH and transforming growth factor beta1-induced connexin43 gap junction formation and steroidogenesis in rat granulosa cells
    Ferng Chun Ke
    Institute of Molecular and Cellular Biology, School of Life Sciences, National Taiwan University, Taipei 106, Taiwan
    J Endocrinol 184:555-66. 2005
    ..In conclusion, this study suggests that Cx43 gap junctions may play a critical role in FSH plus TGF beta1-stimulated progesterone production in rat ovarian granulosa cells...

Research Grants12

  1. MITOCHONDRIAL PROTEINS AND LEYDIG CELL STEROIDOGENESIS
    DOUGLAS STOCCO; Fiscal Year: 2009
    ....
  2. MITOCHONDRIAL PROTEINS AND LEYDIG CELL STEROIDOGENESIS
    DOUGLAS STOCCO; Fiscal Year: 2007
    ....
  3. REGULATION OF REPRODUCTIVE PROCESSES
    DOUGLAS STOCCO; Fiscal Year: 2003
    ..abstract_text> ..
  4. MITOCHONDRIAL PROTEINS AND LEYDIG CELL STEROIDOGENESIS
    DOUGLAS STOCCO; Fiscal Year: 1993
    ..Immunocytochemistry will be performed using the protein-A gold technique. Microsequencing of proteins will be performed by a commercial source. Cloning and sequencing of the gene will be performed using standard methodology...
  5. MITOCHONDRIAL PROTEINS AND LEYDIG CELL STEROIDOGENESIS
    Douglas M Stocco; Fiscal Year: 2010
    ....