A I Scott

Summary

Affiliation: Texas A and M University
Country: USA

Publications

  1. ncbi request reprint Biosynthesis of cobalamin (vitamin B(12))
    A I Scott
    Department of Chemistry, Texas A and M University, College Station, TX 77843 3255, USA
    Biochem Soc Trans 30:613-20. 2002
  2. ncbi request reprint Reflections on the discovery of nature's pathways to vitamin B12
    A I Scott
    Center for Biological NMR, Chemistry Department, Texas A and M University, College Station 77843 3255, USA
    Chem Rec 1:212-27. 2001
  3. ncbi request reprint Overexpression in Escherichia coli of 12 vitamin B12 biosynthetic enzymes
    C A Roessner
    Center for Biological NMR, Department of Chemistry, Texas A and M University, College Station 77843 3255, USA
    Protein Expr Purif 6:155-63. 1995
  4. ncbi request reprint Enzymatic synthesis and structure of precorrin-3, a trimethyldipyrrocorphin intermediate in vitamin B12 biosynthesis
    M J Warren
    Department of Chemistry, Texas A and M University, College Station 77843 3255
    Biochemistry 31:603-9. 1992
  5. pmc Cloning, sequencing, and expression of the uroporphyrinogen III methyltransferase cobA gene of Propionibacterium freudenreichii (shermanii)
    I Sattler
    Center for Biological NMR, Chemistry Department, Texas A and M University, College Station 77843
    J Bacteriol 177:1564-9. 1995
  6. ncbi request reprint The Escherichia coli cysG gene encodes the multifunctional protein, siroheme synthase
    J B Spencer
    Department of Chemistry, Texas A and M University, College Station 77843 3255
    FEBS Lett 335:57-60. 1993
  7. ncbi request reprint Holo-sterol carrier protein-2. (13)C NMR investigation of cholesterol and fatty acid binding sites
    N Stolowich
    Department of Chemistry, Texas A and M University, College Station, Texas 77843 4466, USA
    J Biol Chem 274:35425-33. 1999
  8. ncbi request reprint Enzymatic synthesis of dihydrosirohydrochlorin (precorrin-2) and of a novel pyrrocorphin by uroporphyrinogen III methylase
    M J Warren
    Department of Chemistry, Texas A and M University, College Station 77843
    FEBS Lett 261:76-80. 1990
  9. ncbi request reprint Evidence for an intermediate in the enzymatic formation of uroporphyrinogen III
    C Pichon
    Department of Chemistry, Texas A and M University, College Station 77843 3255
    Bioorg Med Chem 2:153-68. 1994
  10. ncbi request reprint Sterol carrier protein-2: structure reveals function
    N J Stolowich
    Department of Chemistry, University of Louisville, Kentucky 40292, USA
    Cell Mol Life Sci 59:193-212. 2002

Collaborators

Detail Information

Publications24

  1. ncbi request reprint Biosynthesis of cobalamin (vitamin B(12))
    A I Scott
    Department of Chemistry, Texas A and M University, College Station, TX 77843 3255, USA
    Biochem Soc Trans 30:613-20. 2002
    ....
  2. ncbi request reprint Reflections on the discovery of nature's pathways to vitamin B12
    A I Scott
    Center for Biological NMR, Chemistry Department, Texas A and M University, College Station 77843 3255, USA
    Chem Rec 1:212-27. 2001
    ....
  3. ncbi request reprint Overexpression in Escherichia coli of 12 vitamin B12 biosynthetic enzymes
    C A Roessner
    Center for Biological NMR, Department of Chemistry, Texas A and M University, College Station 77843 3255, USA
    Protein Expr Purif 6:155-63. 1995
    ..The activity of each enzyme has been demonstrated by the biosynthesis of hydrogenobyrinic acid from aminolevulinic acid...
  4. ncbi request reprint Enzymatic synthesis and structure of precorrin-3, a trimethyldipyrrocorphin intermediate in vitamin B12 biosynthesis
    M J Warren
    Department of Chemistry, Texas A and M University, College Station 77843 3255
    Biochemistry 31:603-9. 1992
    ..The implications of this structure for corrin biosynthesis are discussed...
  5. pmc Cloning, sequencing, and expression of the uroporphyrinogen III methyltransferase cobA gene of Propionibacterium freudenreichii (shermanii)
    I Sattler
    Center for Biological NMR, Chemistry Department, Texas A and M University, College Station 77843
    J Bacteriol 177:1564-9. 1995
    ..These two genes do not appear to constitute part of an extensive cobalamin operon...
  6. ncbi request reprint The Escherichia coli cysG gene encodes the multifunctional protein, siroheme synthase
    J B Spencer
    Department of Chemistry, Texas A and M University, College Station 77843 3255
    FEBS Lett 335:57-60. 1993
    ..Thus CysG is a multifunctional protein solely responsible for siroheme synthesis from uro'gen III in E. coli, and accordingly is renamed siroheme synthase...
  7. ncbi request reprint Holo-sterol carrier protein-2. (13)C NMR investigation of cholesterol and fatty acid binding sites
    N Stolowich
    Department of Chemistry, Texas A and M University, College Station, Texas 77843 4466, USA
    J Biol Chem 274:35425-33. 1999
    ..These data contribute to our understanding of a role for SCP-2 in both cellular cholesterol and LCFA metabolism...
  8. ncbi request reprint Enzymatic synthesis of dihydrosirohydrochlorin (precorrin-2) and of a novel pyrrocorphin by uroporphyrinogen III methylase
    M J Warren
    Department of Chemistry, Texas A and M University, College Station 77843
    FEBS Lett 261:76-80. 1990
    ....
  9. ncbi request reprint Evidence for an intermediate in the enzymatic formation of uroporphyrinogen III
    C Pichon
    Department of Chemistry, Texas A and M University, College Station 77843 3255
    Bioorg Med Chem 2:153-68. 1994
    ..Using conditions to slow down the enzyme activity (high pH, low temperature), the transient species was trapped with ammonium ions as aminomethylbilane and with sodium borohydride as methylbilane, and observed by 13C-NMR...
  10. ncbi request reprint Sterol carrier protein-2: structure reveals function
    N J Stolowich
    Department of Chemistry, University of Louisville, Kentucky 40292, USA
    Cell Mol Life Sci 59:193-212. 2002
    ..Thus, understanding the structural domains of the SCP-x/pro-SCP-2 gene and its respective posttranslationally processed proteins has provided new insights into their functions in intracellular targeting and metabolism of lipids...
  11. pmc The Escherichia coli cysG gene encodes S-adenosylmethionine-dependent uroporphyrinogen III methylase
    M J Warren
    Department of Chemistry, Texas A and M University, College Station 77843
    Biochem J 265:725-9. 1990
    ..m.r. spectrum of the methyl ester produced by incubation of the methylase with uroporphyrinogen III, derived from [4.6-13C2]porphobilinogen, and S-adenosyl[Me-13C]methionine...
  12. ncbi request reprint Tetrapyrrole assembly and modification into the ligands of biologically functional cofactors
    M J Warren
    Department of Chemistry, Texas A and M University, College Station 77843 3255
    Trends Biochem Sci 15:486-91. 1990
    ....
  13. ncbi request reprint Multiple biosynthetic pathways for vitamin B12: variations on a central theme
    C A Roessner
    Center for Biological NMR, Department of Chemistry, Texas A and M University, College Station, Texas 77843, USA
    Vitam Horm 61:267-97. 2001
    ..The aerobic process utilizes two enzymes and is dependent on molecular oxygen, in stark contrast to the anaerobic mechanism which is controlled by cobalt and requires only one enzyme...
  14. ncbi request reprint Crystal structure of precorrin-8x methyl mutase
    L W Shipman
    Department of Chemistry, Texas A and M University, College Station, TX 77843, USA
    Structure 9:587-96. 2001
    ....
  15. ncbi request reprint 19-Bromo-1-hydroxymethylbilane, a novel inhibitor of uro'gen III synthase
    C Pichon
    Department of Chemistry, Texas A and M University, College Station 77843 3255
    Bioorg Med Chem 2:267-77. 1994
    ..A novel hydroxymethylbilane analog, 19-Br-HMB (11), has been synthesized. Its activity with the enzyme Uro'gen III synthase shows competitive inhibition...
  16. ncbi request reprint Mutagenesis identifies a conserved tyrosine residue important for the activity of uroporphyrinogen III synthase from Anacystis nidulans
    Charles A Roessner
    Center for Biological NMR, Department of Chemsitry, P O Box 30012, Texas A and M University, College Station, TX 77842 3012, USA
    FEBS Lett 525:25-8. 2002
    ..Of the nine conserved amino acids altered, only a single tyrosine mutant (Y166F) showed any significant decrease in activity suggesting this residue is critical for proper substrate binding and/or catalysis...
  17. ncbi request reprint The sterol carrier protein-2 fatty acid binding site: an NMR, circular dichroic, and fluorescence spectroscopic determination
    N J Stolowich
    Department of Chemistry, Texas A and M University, College Station 77843 3255, USA
    Biochemistry 36:1719-29. 1997
    ..The pH-induced changes in SCP-2 secondary structure and ligand binding activity may be important to the mechanism whereby this protein interacts with membrane surfaces to enhance lipid binding/transfer...
  18. ncbi request reprint Enzymatic synthesis of S-adenosyl-L-methionine on the preparative scale
    J Park
    Center for Biological NMR, Department of Chemistry, Texas A and M University, College Station 77843 3255, USA
    Bioorg Med Chem 4:2179-85. 1996
    ..coli enzyme in the presence of additives such as beta ME, acetonitrile, urea, or pTsONa. The recombinant yeast SAM synthetase was used to generate SAM in situ for use in the multi-enzymatic synthesis of precorrin 2...
  19. pmc Biosynthesis of vitamin B12: factor IV, a new intermediate in the anaerobic pathway
    A I Scott
    Department of Chemistry, Texas A and M University, College Station 77843 3255, USA
    Proc Natl Acad Sci U S A 93:14316-9. 1996
    ..Incorporation of radiolabeled factor IV into cobyrinic acid established the biointermediacy of this cobalt complex, whose structure has implications for the mechanisms of the anaerobic pathway to B12...
  20. ncbi request reprint Engineering Escherichia coli for the synthesis of taxadiene, a key intermediate in the biosynthesis of taxol
    Q Huang
    Center for Biological NMR, Department of Chemistry, PO Box 30012, Texas A and M University, College Station, TX 77842-3012, USA
    Bioorg Med Chem 9:2237-42. 2001
    ..3mg per liter of cell culture. The success of both in vitro and in vivo synthesis of taxadiene bodes well for the future production of taxoids by non-paclitaxel producing organisms through pathway engineering...
  21. ncbi request reprint Expression of 9 Salmonella typhimurium enzymes for cobinamide synthesis. Identification of the 11-methyl and 20-methyl transferases of corrin biosynthesis
    C A Roessner
    Department of Chemistry, Texas A and M University, College Station 77843 3255
    FEBS Lett 301:73-8. 1992
    ..The enzymatic functions of the gene products of cbiL and cbiF are associated with C-methylation at C-20 of precorrin-2 and C-11 of precorrin-3...
  22. ncbi request reprint An improved synthesis of the dinucleotides pdCpA and pdCpdA
    X F Zhu
    Center for Biological NMR, Department of Chemistry, Texas A and M University, College Station 77842-3012, USA
    Nucleosides Nucleotides Nucleic Acids 20:197-211. 2001
    ..The utility and efficiency of this approach has been further demonstrated by its application to the synthesis of total DNA dinucleotide pdCpdA 17 and total RNA dinucleotide 21...
  23. pmc Gene dissection demonstrates that the Escherichia coli cysG gene encodes a multifunctional protein
    M J Warren
    School of Biological Sciences, Queen Mary and Westfield College, London, U K
    Biochem J 302:837-44. 1994
    ..The evidence presented in this paper suggests that the CysG protein is a multifunctional protein involved in SAM-dependent methylation, pyridine dinucleotide dependent dehydrogenation and ferrochelation...
  24. ncbi request reprint Overexpression, purification, and characterization of the thermostable mevalonate kinase from Methanococcus jannaschii
    K X Huang
    Department of Biochemistry and Cell Biology, Rice University, 6100 Main Street, Houston, Texas 77005 1892, USA
    Protein Expr Purif 17:33-40. 1999
    ..8. The Michaelis constant (K(m)) for (RS)-mevalonate was 68.5 microM and was 92 microM for ATP. The V(max) was 387 units mg(-1). The optimal temperature for mevalonate kinase activity was 70-75 degrees C...