Ke He Ruan

Summary

Affiliation: Texas Medical Center
Country: USA

Publications

  1. ncbi request reprint Implications of the molecular basis of prostacyclin biosynthesis and signaling in pharmaceutical designs
    Ke He Ruan
    The Vascular Biology Research Center, Division of Hematology, Department of Internal Medicine, University of Texas Health Science Center at Houston, 77030, USA
    Curr Pharm Des 12:925-41. 2006
  2. pmc Solution structure and topology of the N-terminal membrane anchor domain of a microsomal cytochrome P450: prostaglandin I2 synthase
    Ke He Ruan
    The Vascular Biology Research Center and Division of Hematology, Department of Internal Medicine, The University of Texas Health Science Center, Houston, TX 77030, USA
    Biochem J 368:721-8. 2002
  3. ncbi request reprint Solution structure of a common substrate mimetic of cyclooxygenase-downstream synthases bound to an engineered thromboxane A2 synthase using a high-resolution NMR technique
    Ke He Ruan
    Vascular Biology Research Center, Division of Hematology, Department of Internal Medicine, The University of Texas Health Science Center, Houston, 77030, USA
    Arch Biochem Biophys 444:165-73. 2005
  4. ncbi request reprint The N-terminal membrane anchor domain of the membrane-bound prostacyclin synthase involved in the substrate presentation of the coupling reaction with cyclooxygenase
    Ke He Ruan
    Department of Internal Medicine, The University of Texas Health Science Center, Houston, TX 77030, USA
    Arch Biochem Biophys 435:372-81. 2005
  5. ncbi request reprint Advance in understanding the biosynthesis of prostacyclin and thromboxane A2 in the endoplasmic reticulum membrane via the cyclooxygenase pathway
    Ke He Ruan
    The Vascular Biology Research Center and Division of Hematology, Department of Internal Medicine, The University of Texas Health Science Center, Houston, Texas 77030, USA
    Mini Rev Med Chem 4:639-47. 2004
  6. ncbi request reprint NMR structure of the thromboxane A2 receptor ligand recognition pocket
    Ke He Ruan
    Vascular Biology Research Center and Division of Hematology, Department of Internal Medicine, The University of Texas Health Science Center, Houston, TX 77030, USA
    Eur J Biochem 271:3006-16. 2004
  7. ncbi request reprint Evidence of the residues involved in ligand recognition in the second extracellular loop of the prostacyclin receptor characterized by high resolution 2D NMR techniques
    Ke He Ruan
    Vascular Biology Research Center and Division of Hematology, Department of Internal Medicine, The University of Texas Health Science Center, Houston, TX 77030, USA
    Arch Biochem Biophys 418:25-33. 2003
  8. ncbi request reprint Identification of the residues in the helix F/G loop important to catalytic function of membrane-bound prostacyclin synthase
    Hui Deng
    The Vascular Biology Research Center and Division of Hematology, Department of Internal Medicine, The University of Texas Health Science Center, Houston, Texas 77030, USA
    Biochemistry 42:5609-17. 2003
  9. pmc Substrate access channel topology in membrane-bound prostacyclin synthase
    Hui Deng
    The Vascular Biology Research Center and Division of Hematology, Department of Internal Medicine, The University of Texas Health Science Center, 6431 Fannin Street, Houston, TX 77030, U S A
    Biochem J 362:545-51. 2002
  10. ncbi request reprint Structural and functional characterization of the first intracellular loop of human thromboxane A2 receptor
    Linda Geng
    Vascular Biology Research Center and Division of Hematology, Department of Internal Medicine, The University of Texas Health Science Center, Houston, Texas 77030, USA
    Arch Biochem Biophys 423:253-65. 2004

Collaborators

Detail Information

Publications40

  1. ncbi request reprint Implications of the molecular basis of prostacyclin biosynthesis and signaling in pharmaceutical designs
    Ke He Ruan
    The Vascular Biology Research Center, Division of Hematology, Department of Internal Medicine, University of Texas Health Science Center at Houston, 77030, USA
    Curr Pharm Des 12:925-41. 2006
    ....
  2. pmc Solution structure and topology of the N-terminal membrane anchor domain of a microsomal cytochrome P450: prostaglandin I2 synthase
    Ke He Ruan
    The Vascular Biology Research Center and Division of Hematology, Department of Internal Medicine, The University of Texas Health Science Center, Houston, TX 77030, USA
    Biochem J 368:721-8. 2002
    ..The distance between residues 1 and 20 is approx. 20 A (1 A=0.1 nm), less than the thickness of a lipid bilayer. This indicates that the N-terminal membrane anchor domain of prostaglandin I(2) synthase does not penetrate the ER membrane...
  3. ncbi request reprint Solution structure of a common substrate mimetic of cyclooxygenase-downstream synthases bound to an engineered thromboxane A2 synthase using a high-resolution NMR technique
    Ke He Ruan
    Vascular Biology Research Center, Division of Hematology, Department of Internal Medicine, The University of Texas Health Science Center, Houston, 77030, USA
    Arch Biochem Biophys 444:165-73. 2005
    ..The NMR technique can be used as a powerful tool to determine the conformations of PGH(2) bound to other COX-downstream synthases...
  4. ncbi request reprint The N-terminal membrane anchor domain of the membrane-bound prostacyclin synthase involved in the substrate presentation of the coupling reaction with cyclooxygenase
    Ke He Ruan
    Department of Internal Medicine, The University of Texas Health Science Center, Houston, TX 77030, USA
    Arch Biochem Biophys 435:372-81. 2005
    ..The TXAS N-terminal domain could not replace the function of the corresponding domain of PGIS, indicating that the facilitation of the substrate presentation is specific...
  5. ncbi request reprint Advance in understanding the biosynthesis of prostacyclin and thromboxane A2 in the endoplasmic reticulum membrane via the cyclooxygenase pathway
    Ke He Ruan
    The Vascular Biology Research Center and Division of Hematology, Department of Internal Medicine, The University of Texas Health Science Center, Houston, Texas 77030, USA
    Mini Rev Med Chem 4:639-47. 2004
    ....
  6. ncbi request reprint NMR structure of the thromboxane A2 receptor ligand recognition pocket
    Ke He Ruan
    Vascular Biology Research Center and Division of Hematology, Department of Internal Medicine, The University of Texas Health Science Center, Houston, TX 77030, USA
    Eur J Biochem 271:3006-16. 2004
    ..This molecular mechanism of ligand recognition is the first that may be applied to other prostanoid receptors and other GPCRs...
  7. ncbi request reprint Evidence of the residues involved in ligand recognition in the second extracellular loop of the prostacyclin receptor characterized by high resolution 2D NMR techniques
    Ke He Ruan
    Vascular Biology Research Center and Division of Hematology, Department of Internal Medicine, The University of Texas Health Science Center, Houston, TX 77030, USA
    Arch Biochem Biophys 418:25-33. 2003
    ....
  8. ncbi request reprint Identification of the residues in the helix F/G loop important to catalytic function of membrane-bound prostacyclin synthase
    Hui Deng
    The Vascular Biology Research Center and Division of Hematology, Department of Internal Medicine, The University of Texas Health Science Center, Houston, Texas 77030, USA
    Biochemistry 42:5609-17. 2003
    ....
  9. pmc Substrate access channel topology in membrane-bound prostacyclin synthase
    Hui Deng
    The Vascular Biology Research Center and Division of Hematology, Department of Internal Medicine, The University of Texas Health Science Center, 6431 Fannin Street, Houston, TX 77030, U S A
    Biochem J 362:545-51. 2002
    ..These observations support our topology model in which the PGIS substrate access channel opening is positioned close to the ER membrane...
  10. ncbi request reprint Structural and functional characterization of the first intracellular loop of human thromboxane A2 receptor
    Linda Geng
    Vascular Biology Research Center and Division of Hematology, Department of Internal Medicine, The University of Texas Health Science Center, Houston, Texas 77030, USA
    Arch Biochem Biophys 423:253-65. 2004
    ..The difference between the side chain functions of Lys and Arg in effecting the signaling was discussed...
  11. ncbi request reprint Identification of residues important for ligand binding of thromboxane A2 receptor in the second extracellular loop using the NMR experiment-guided mutagenesis approach
    Shui Ping So
    Vascular Biology Research Center and Division of Hematology, Department of Internal Medicine, The University of Texas Health Science Center, Houston, Texas 77030, USA
    J Biol Chem 278:10922-7. 2003
    ..This information facilitates the understanding of the molecular mechanism of thromboxane A(2) binding to the important receptor and its signal transduction...
  12. ncbi request reprint Characterization of the substrate mimic bound to engineered prostacyclin synthase in solution using high-resolution NMR spectroscopy and mutagenesis: implication of the molecular mechanism in biosynthesis of prostacyclin
    Ke He Ruan
    The Center for Experimental Therapeutics and PharmacoInformatics and Department of Pharmacological and Pharmaceutical Sciences, College of Pharmacy, University of Houston, Houston, Texas 77204, USA
    Biochemistry 47:680-8. 2008
    ....
  13. ncbi request reprint A profile of the residues in the first intracellular loop critical for Gs-mediated signaling of human prostacyclin receptor characterized by an integrative approach of NMR-experiment and mutagenesis
    Lihai Zhang
    Vascular Biology Research Center and Division of Hematology, Department of Internal Medicine, The University of Texas Health Science Center, Houston, Texas 77030, USA
    Biochemistry 44:11389-401. 2005
    ..The conservation of the basic residues (Arg45 in the IP) in all of the prostanoid receptors suggests that the iLP1 regions of the other prostanoid receptors may also contain the epitopes important to their signaling...
  14. ncbi request reprint Solution structure of the third extracellular loop of human thromboxane A2 receptor
    Jiaxin Wu
    Vascular Biology Research Center and Division of Hematology, Department of Internal Medicine, The University of Texas Health Science Center, 6431 Fannin St, Houston, TX 77030, USA
    Arch Biochem Biophys 414:287-93. 2003
    ..These results provide valuable information for the characterization of the complete 3D structure of the extracellular domains of the human TP receptor...
  15. ncbi request reprint Determination of the membrane contact residues and solution structure of the helix F/G loop of prostaglandin I2 synthase
    Jiaxin Wu
    Vascular Biology Research Center and Division of Hematology, Department of Internal Medicine, The University of Texas Health Science Center, 6431 Fannin St, Houston, TX 77030, USA
    Arch Biochem Biophys 411:27-35. 2003
    ....
  16. pmc A simple, quick, and high-yield preparation of the human thromboxane A2 receptor in full size for structural studies
    Ke He Ruan
    Department of Pharmacological and Pharmaceutical Sciences, Center for Experimental Therapeutics and Pharmacoinformatics, University of Houston, Houston, Texas 77204 5037, USA
    Biochemistry 47:6819-26. 2008
    ..These studies provide a basis for the high-yield expression and purification of the GPCR for the structural and functional characterization using biophysics approaches...
  17. pmc Large-scale expression, purification, and characterization of an engineered prostacyclin-synthesizing enzyme with therapeutic potential
    Ke He Ruan
    Department of Pharmacological and Pharmaceutical Sciences, Center for Experimental Therapeutics and Pharmacoinformatics, College of Pharmacy, University of Houston, Science and Research Building 2, Room 521, Houston, TX 77204 5037, USA
    Arch Biochem Biophys 480:41-50. 2008
    ..These studies have clearly demonstrated that the isolated TriCat enzyme protein functions in the selective biosynthesis of the vascular protector, PGI(2), and revealed its potential for anti-thrombosis therapeutics...
  18. ncbi request reprint Prostacyclin is an autocrine regulator in the contraction of oviductal smooth muscle
    Farinaz Arbab
    Department of Pathology, Baylor College of Medicine, University of Texas Health Science Center at Houston, Texas, USA
    Hum Reprod 17:3053-9. 2002
    ..It is well established that prostacyclin relaxes vascular smooth muscle, but whether oviductal smooth muscle synthesizes prostacyclin and whether its contraction is affected by prostacyclin remain unclear...
  19. ncbi request reprint Structural and functional analysis of the C-terminus of Galphaq in complex with the human thromboxane A2 receptor provides evidence of constitutive activity
    Annirudha Chillar
    Center for Experimental Therapeutics and Pharmacoinformatics, Department of Pharmacological and Pharmaceutical Sciences, University of Houston, Houston, TX 77204, USA
    Biochemistry 49:6365-74. 2010
    ..The study indicates a promising new outlook for the examination of prostanoid receptor-G-protein interactions in greater detail using integrated NMR spectroscopy, the purified receptor, and the stable cell line...
  20. pmc Assembling NMR structures for the intracellular loops of the human thromboxane A2 receptor: implication of the G protein-coupling pocket
    Jiaxin Wu
    The Center for Experimental Therapeutics and PharmacoInformatics, Department of Pharmacological and Pharmaceutical Sciences, College of Pharmacy, University of Houston, Room 521 Science and Research Building 2, Houston, TX 77204 5037, USA
    Arch Biochem Biophys 470:73-82. 2008
    ..These results reveal the possibly important molecular mechanisms in TP signaling and provide structural information to characterize other prostanoid receptor signalings...
  21. ncbi request reprint Engineering of a protein with cyclooxygenase and prostacyclin synthase activities that converts arachidonic acid to prostacyclin
    Ke He Ruan
    Vascular Biology Research Center and Division of Hematology, Department of Internal Medicine, The University of Texas Health Science Center, Houston, Texas 77030, USA
    Biochemistry 45:14003-11. 2006
    ..The studies have also provided a model linking COX with its downstream enzymes to specifically regulate biosynthesis of eicosanoids which have potent biological functions...
  22. doi request reprint Endothelial-like progenitor cells engineered to produce prostacyclin rescue monocrotaline-induced pulmonary arterial hypertension and provide right ventricle benefits
    Lei Zhou
    Department of Molecular Cardiology, Texas Heart Institute, Houston, TX 77030, USA
    Circulation 128:982-94. 2013
    ..We hypothesize that localized jugular vein delivery of prostacyclin-producing cells may provide sustained therapeutic effects without the limitations of systemic delivery...
  23. pmc A strategy using NMR peptide structures of thromboxane A2 receptor as templates to construct ligand-recognition pocket of prostacyclin receptor
    Cheng Huai Ruan
    Vascular Biology Research Center, Division of Hematology, Department of Internal Medicine, The University of Texas Health Science Center, Houston, Texas 77030, USA
    BMC Biochem 6:23. 2005
    ..The studies have identified the segment along with several residues in the eLP domains important to ligand recognition, as well as proposed a ligand recognition pocket for the TP receptor...
  24. pmc Characterization of the prostaglandin H2 mimic: binding to the purified human thromboxane A2 receptor in solution
    Ke He Ruan
    Center for Experimental Therapeutics and Pharmacoinformatics, Department of Pharmacological and Pharmaceutical Sciences, College of Pharmacy, University of Houston, 4800 Calhoun Rd S and R II Bldg, Houston, TX 77204, USA
    Arch Biochem Biophys 477:396-403. 2008
    ..The results also provided sufficient information for speculating the molecular basis of how PGH(2) binds to multiple target proteins even though unrelated in their protein sequences...
  25. pmc Engineering of a novel hybrid enzyme: an anti-inflammatory drug target with triple catalytic activities directly converting arachidonic acid into the inflammatory prostaglandin E2
    Ke He Ruan
    Department of Pharmacological and Pharmaceutical Sciences, The Center for Experimental Therapeutics and PharmacoInformatics, University of Houston, College of Pharmacy, Houston, TX 77030, USA
    Protein Eng Des Sel 22:733-40. 2009
    ..The COX-2-10aa-mPGES-1 hybrid enzyme engineering may be a novel approach in creating inflammation cell and animal models, which are particularly valuable targets for the next generation of NSAID screening...
  26. doi request reprint Ligand-specific conformation determines agonist activation and antagonist blockade in purified human thromboxane A2 receptor
    Ke He Ruan
    The Center for Experimental Therapeutics and PharmacoInformatics, Department of Pharmacological and Pharmaceutical Sciences, College of Pharmacy, 521 Science and Research Building 2, University of Houston, Houston, Texas 77004 5037, USA
    Biochemistry 48:3157-65. 2009
    ..These studies are the first to show a possible mechanism of the ligand-specific conformation-dependent agonist activation and antagonist blockage in the GPCR...
  27. ncbi request reprint Characterization of the molecular mechanisms of the coupling between intracellular loops of prostacyclin receptor with the C-terminal domain of the Galphas protein in human coronary artery smooth muscle cells
    Lihai Zhang
    Vascular Biology Research Center and Division of Hematology, Department of Internal Medicine, The University of Texas Health Science Center, Houston, TX 77030, USA
    Arch Biochem Biophys 454:80-8. 2006
    ....
  28. pmc Involvement of non-conserved residues important for PGE2 binding to the constrained EP3 eLP2 using NMR and site-directed mutagenesis
    Annirudha Chillar
    Center for Experimental Therapeutics and Pharmacoinformatics, and Department of Pharmacological and Pharmaceutical Sciences, S and R 2, College of Pharmacy, University of Houston, Houston, TX 77204 5037, USA
    FEBS Lett 582:2863-8. 2008
    ....
  29. doi request reprint Establishing novel prostacyclin-synthesizing cells with therapeutic potential against heart diseases
    Ke He Ruan
    Center for Experimental Therapeutics and Pharmacoinformatics, Department of Pharmacological and Pharmaceutical Sciences, University of Houston, Houston, TX, USA
    Int J Cardiol 163:163-9. 2013
    ..For decades, there have been many ongoing attempts to use prostaglandin I(2) (PGI(2)) to treat heart diseases, such as pulmonary arterial hypertension. However, the short half life of PGI(2) has limited the therapeutic impact potential...
  30. pmc Engineered endothelial progenitor cells that overexpress prostacyclin protect vascular cells
    Qi Liu
    the Texas Heart Institute at St Luke s Episcopal Hospital, Houston, TX, USA
    J Cell Physiol 227:2907-16. 2012
    ..PGI2-EPCs showed enhanced intrinsic proangiogenic properties and provided favorable paracrine-mediated cellular protections, including promoting in vitro angiogenesis of native EPCs and hyperpolarization of SMCs under hypoxia...
  31. pmc Prostacyclin therapy for pulmonary arterial hypertension
    Cheng Huai Ruan
    Department of Internal Medicine, New York Hospital Medical Center of Queens Weil Cornell Medical College Affiliated Hospital, Flushing, New York 11355, USA
    Tex Heart Inst J 37:391-9. 2010
    ..By using the recent advances in technology and the molecular understanding of prostacyclin synthesis, researchers are prepared to make significant advances in the treatment of pulmonary arterial hypertension...
  32. ncbi request reprint Solution structure of the first intracellular loop of prostacyclin receptor and implication of its interaction with the C-terminal segment of G alpha s protein
    Lihai Zhang
    Vascular Biology Research Center and Division of Hematology, Department of Internal Medicine, The University of Texas Health Science Center, Houston, Texas 77030, USA
    Biochemistry 45:1734-44. 2006
    ..The possible charge and hydrophobic interactions between the two peptides were also discussed. These data prompted intriguing speculations on the IP/G alpha s coupling which mediates vasodilatation and inhibition of platelet aggregation...
  33. ncbi request reprint Prunella stica inhibits the proliferation but not the prostaglandin production of Ishikawa cells
    Jaou Chen Huang
    Department of Obstetrics and Gynecology, University of Texas Health Science Center, 6431 Fannin Street, Houston, TX 77030, USA
    Life Sci 79:436-41. 2006
    ..The therapeutic effects of P. stica reside, in part, in inhibiting the proliferation of the epithelial cells derived from human endometrium. The active components are small molecules...
  34. ncbi request reprint COX-2-10aa-PGIS gene therapy improves erectile function in rats after cavernous nerve injury
    Haocheng Lin
    Department of Urology, Affiliated Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing, China
    J Sex Med 10:1476-87. 2013
    ..COX-2-10aa-PGIS is a newly engineered protein with COX-2 and prostacyclin synthase activities that converts arachidonic acid directly to prostacyclin (prostaglandin I2 [PGI2]). PGI2 is a potent smooth muscle relaxant...
  35. pmc Physical and functional antagonism between tumor suppressor protein p53 and fortilin, an anti-apoptotic protein
    Yanjie Chen
    Division of Cardiology, Department of Internal Medicine, University of Texas Medical Branch, Galveston, Texas 77555, USA
    J Biol Chem 286:32575-85. 2011
    ..Our results suggest that fortilin is a novel p53-interacting molecule and p53 inhibitor and that it is a logical molecular target in cancer therapy...
  36. ncbi request reprint Purification and characterization of a cyclooxygenase-2 and angiogenesis suppressing factor produced by human fibroblasts
    Wu Guo Deng
    Vascular Biology Research Center, Institute of Molecular Medicine, and Division of Hematology, University of Texas Houston Medical School, Houston, Texas, USA
    FASEB J 16:1286-8. 2002
    ..These findings provide evidence for the control of COX-2 transcription by an endogenous cellular factor...
  37. doi request reprint A new strategy, SuperEnzyme gene therapy in penile rehabilitation
    Jiuhong Yuan
    University of Texas Health Science Center at Houston and MD Anderson Cancer Center, Division of Urology, Houston, TX 77030, USA
    J Sex Med 6:328-33. 2009
    ..Transfection of SuperEnzyme into the penis to generate high levels of PGI(2) may increase penile blood inflow, alleviate hypoxia, and prevent apoptosis and fibrosis with potential use for ED after RP...
  38. ncbi request reprint From the design to the clinical application of thromboxane modulators
    Jean Michel Dogne
    Natural and Synthetic Drug Research Centre, Medicinal Chemistry, University of Liege, Belgium
    Curr Pharm Des 12:903-23. 2006
    ..Finally, in the last part we will highlight the latest perspectives in drug design of thromboxane modulators and in their future therapeutic applications such as cancer, metastasis and angiogenesis...
  39. pmc A profile of the residues in the second extracellular loop that are critical for ligand recognition of human prostacyclin receptor
    Feng Ni
    The Department of Pharmacological and Pharmaceutical Sciences, and The Center for Experimental Therapeutics and PharmacoInformatics, University of Houston, TX 77204 5037, USA
    FEBS J 275:128-37. 2008
    ....
  40. ncbi request reprint New developments on thromboxane and prostacyclin modulators part I: thromboxane modulators
    Jean Michel Dogne
    Natural and Synthetic Drug Research Center, Medicinal Chemistry, University of Liege, 1, Av de l Hôpital, B 4000 Liege, Belgium
    Curr Med Chem 11:1223-41. 2004
    ..In this review, we will propose a description of the recently described thromboxane modulators of major interest from both a pharmacological and a chemical point of view...