Stephen C Pflugfelder

Summary

Affiliation: Texas Medical Center
Country: USA

Publications

  1. ncbi request reprint Antiinflammatory therapy for dry eye
    Stephen C Pflugfelder
    Ocular Surface Center, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas 77030, USA
    Am J Ophthalmol 137:337-42. 2004
  2. ncbi request reprint Immunoregulation on the ocular surface: 2nd Cullen Symposium
    Stephen C Pflugfelder
    Ophthalmology Ocular Surface Center, Cullen Eye Institute, Houston, TX 77030, USA
    Ocul Surf 7:67-77. 2009
  3. ncbi request reprint Correlation of the Schirmer 1 and fluorescein clearance tests with the severity of corneal epithelial and eyelid disease
    A Macri
    Cullen Eye Institute, 6565 Fannin St, NC 205, Houston, TX 77030, USA
    Arch Ophthalmol 118:1632-8. 2000
  4. doi request reprint Effects of sequential artificial tear and cyclosporine emulsion therapy on conjunctival goblet cell density and transforming growth factor-beta2 production
    Stephen C Pflugfelder
    Ocular Surface Center, Baylor College of Medicine, Houston, TX, USA
    Cornea 27:64-9. 2008
  5. pmc Matrix metalloproteinase-9 knockout confers resistance to corneal epithelial barrier disruption in experimental dry eye
    Stephen C Pflugfelder
    Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA
    Am J Pathol 166:61-71. 2005
  6. pmc Cleavage of functional IL-2 receptor alpha chain (CD25) from murine corneal and conjunctival epithelia by MMP-9
    Cintia S de Paiva
    Ocular Surface Center, Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine, Houston, Texas, USA
    J Inflamm (Lond) 6:31. 2009
  7. ncbi request reprint Future directions in therapeutic interventions for conjunctival inflammatory disorders
    Stephen C Pflugfelder
    Ocular Surface Center, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas, USA
    Curr Opin Allergy Clin Immunol 7:450-3. 2007
  8. ncbi request reprint Corneal thickness indices discriminate between keratoconus and contact lens-induced corneal thinning
    Stephen C Pflugfelder
    Ocular Surface Center, Cullen Eye Institute, Baylor College of Medicine, Houston, Texas 77030, USA
    Ophthalmology 109:2336-41. 2002
  9. pmc Epithelial-immune cell interaction in dry eye
    Stephen C Pflugfelder
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA
    Cornea 27:S9-11. 2008
  10. pmc Partial enrichment of a population of human limbal epithelial cells with putative stem cell properties based on collagen type IV adhesiveness
    De Quan Li
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, 6565 Fannin Street, NC 205, Houston, TX 77030, USA
    Exp Eye Res 80:581-90. 2005

Research Grants

  1. PATHOGENESIS OF CONJUNCTIVAL SQUAMOUS METAPLASIA
    STEPHEN PFLUGFELDER; Fiscal Year: 2000
  2. PATHOGENESIS OF CONJUNCTIVAL SQUAMOUS METAPLASIA
    STEPHEN CARL PFLUGFELDER; Fiscal Year: 2010
  3. PATHOGENESIS OF CONJUNCTIVAL SQUAMOUS METAPLASIA
    STEPHEN PFLUGFELDER; Fiscal Year: 2009
  4. Environmentally-Induced Ocular Surface Inflammation Mimicking Sjogren's Syndrome
    STEPHEN PFLUGFELDER; Fiscal Year: 2009
  5. PATHOGENESIS OF CONJUNCTIVAL SQUAMOUS METAPLASIA
    STEPHEN PFLUGFELDER; Fiscal Year: 2007
  6. Pathogenesis of Conjunctival Squamous
    STEPHEN PFLUGFELDER; Fiscal Year: 2005
  7. Pathogenesis of Conjunctival Squamous
    STEPHEN PFLUGFELDER; Fiscal Year: 2004
  8. Pathogenesis of Conjunctival Squamous
    STEPHEN PFLUGFELDER; Fiscal Year: 2003
  9. Pathogenesis of Conjunctival Squamous
    STEPHEN PFLUGFELDER; Fiscal Year: 2002
  10. Environmentally-Induced Ocular Surface Inflammation Mimicking Sjogren's Syndrome
    Michael J Stern; Fiscal Year: 2010

Collaborators

Detail Information

Publications70

  1. ncbi request reprint Antiinflammatory therapy for dry eye
    Stephen C Pflugfelder
    Ocular Surface Center, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas 77030, USA
    Am J Ophthalmol 137:337-42. 2004
    ..To present evidence establishing the relationship between inflammation and dry eye and supporting the use of antiinflammatory therapy for dry eye...
  2. ncbi request reprint Immunoregulation on the ocular surface: 2nd Cullen Symposium
    Stephen C Pflugfelder
    Ophthalmology Ocular Surface Center, Cullen Eye Institute, Houston, TX 77030, USA
    Ocul Surf 7:67-77. 2009
    ..The ocular surface is an immunologically active mucosal site that contains numerous mechanisms to regulate the immune response to prevent tissue destruction and vision loss...
  3. ncbi request reprint Correlation of the Schirmer 1 and fluorescein clearance tests with the severity of corneal epithelial and eyelid disease
    A Macri
    Cullen Eye Institute, 6565 Fannin St, NC 205, Houston, TX 77030, USA
    Arch Ophthalmol 118:1632-8. 2000
    ....
  4. doi request reprint Effects of sequential artificial tear and cyclosporine emulsion therapy on conjunctival goblet cell density and transforming growth factor-beta2 production
    Stephen C Pflugfelder
    Ocular Surface Center, Baylor College of Medicine, Houston, TX, USA
    Cornea 27:64-9. 2008
    ..To evaluate the effects of sequential treatment with artificial tears and cyclosporine emulsion on conjunctival goblet cell density and production of transforming growth factor (TGF)-beta2 in patients with dry eye disease...
  5. pmc Matrix metalloproteinase-9 knockout confers resistance to corneal epithelial barrier disruption in experimental dry eye
    Stephen C Pflugfelder
    Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA
    Am J Pathol 166:61-71. 2005
    ..This appears to be because of accelerated loss of tight junction bearing superficial corneal epithelial cells, perhaps by proteolytic cleavage of occludin...
  6. pmc Cleavage of functional IL-2 receptor alpha chain (CD25) from murine corneal and conjunctival epithelia by MMP-9
    Cintia S de Paiva
    Ocular Surface Center, Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine, Houston, Texas, USA
    J Inflamm (Lond) 6:31. 2009
    ..Soluble IL-2Ralpha (CD25) has been reported as an inflammatory marker. We evaluated the expression of CD25 and CD122 in the ocular surface epithelium and investigated the mechanism of proteolytic cleavage of CD25 from these cells...
  7. ncbi request reprint Future directions in therapeutic interventions for conjunctival inflammatory disorders
    Stephen C Pflugfelder
    Ocular Surface Center, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas, USA
    Curr Opin Allergy Clin Immunol 7:450-3. 2007
    ..To review recent advances in pathogenesis and treatment of conjunctival inflammatory disorders...
  8. ncbi request reprint Corneal thickness indices discriminate between keratoconus and contact lens-induced corneal thinning
    Stephen C Pflugfelder
    Ocular Surface Center, Cullen Eye Institute, Baylor College of Medicine, Houston, Texas 77030, USA
    Ophthalmology 109:2336-41. 2002
    ..The corneal thickness index (CTI) was used to compare central and peripheral corneal thicknesses. The discriminant function 1 (DF1) was used to evaluate corneal thickness and central keratometry measurements...
  9. pmc Epithelial-immune cell interaction in dry eye
    Stephen C Pflugfelder
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA
    Cornea 27:S9-11. 2008
    ..On the other hand, they are targets of cytokines produced by activated T cells that are recruited to the ocular surface in response to dry eye. This interaction between epithelial and immune cells in dry eye will be thoroughly reviewed...
  10. pmc Partial enrichment of a population of human limbal epithelial cells with putative stem cell properties based on collagen type IV adhesiveness
    De Quan Li
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, 6565 Fannin Street, NC 205, Houston, TX 77030, USA
    Exp Eye Res 80:581-90. 2005
    ..The RAC population enriched for certain putative stem cell properties may prove useful in the future for transplantation to diseased and damaged corneas with limbal stem cell deficiency...
  11. pmc Disruption of TGF-β signaling improves ocular surface epithelial disease in experimental autoimmune keratoconjunctivitis sicca
    Cintia S de Paiva
    Ocular Surface Center, Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine, Houston, Texas, United States of America
    PLoS ONE 6:e29017. 2011
    ..These mice have a truncated TGF-β receptor in CD4(+) T cells, rendering them unresponsive to TGF-β...
  12. doi request reprint Hyperosmolarity-induced cornification of human corneal epithelial cells is regulated by JNK MAPK
    Zhuo Chen
    Department of Ophthalmology, The Ocular Surface Center, Cullen Eye Institute, Baylor College of Medicine, Houston, TX 77030, USA
    Invest Ophthalmol Vis Sci 49:539-49. 2008
    ....
  13. ncbi request reprint Apical corneal barrier disruption in experimental murine dry eye is abrogated by methylprednisolone and doxycycline
    Cintia S de Paiva
    Ocular Surface Center, Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine, Houston, Texas 77030, USA
    Invest Ophthalmol Vis Sci 47:2847-56. 2006
    ..To evaluate the mechanism of apical corneal epithelial barrier disruption in response to experimental ocular surface desiccation and the effects of two anti-inflammatory agents (methylprednisolone and doxycycline) on this process...
  14. pmc Essential role for c-Jun N-terminal kinase 2 in corneal epithelial response to desiccating stress
    Cintia S de Paiva
    Ocular Surface Center, Cullen Eye Institute, Baylor College of Medicine, 6565 Fannin St, NC 205, Houston, TX 77030, USA
    Arch Ophthalmol 127:1625-31. 2009
    ..To investigate the protective effects of c-Jun N-terminal kinase (JNK)-1 and -2 gene knockout (KO) on the corneal epithelial response to desiccating stress...
  15. ncbi request reprint Dry eye-induced conjunctival epithelial squamous metaplasia is modulated by interferon-gamma
    Cintia S de Paiva
    Ocular Surface Center, Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine, Houston, Texas 77030, USA
    Invest Ophthalmol Vis Sci 48:2553-60. 2007
    ..To investigate the role of interferon (IFN)-gamma in the pathogenesis of conjunctival squamous metaplasia in dry eye...
  16. pmc Desiccating stress promotion of Th17 differentiation by ocular surface tissues through a dendritic cell-mediated pathway
    Xiaofen Zheng
    Department of Ophthalmology, Baylor College of Medicine, Ocular Surface Center, Cullen Eye Institute, Houston, Texas 77030, USA
    Invest Ophthalmol Vis Sci 51:3083-91. 2010
    ..To explore the phenomenon that corneal and conjunctival tissues subjected to desiccating stress (DS) promote Th17 differentiation by stimulating the production of Th17-inducing cytokines through a dendritic cell (DC)-mediated pathway...
  17. pmc JNK and ERK MAP kinases mediate induction of IL-1beta, TNF-alpha and IL-8 following hyperosmolar stress in human limbal epithelial cells
    De Quan Li
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, 6565 Fannin Street, NC 205, Houston, TX 77030, USA
    Exp Eye Res 82:588-96. 2006
    ..The efficacy of doxycycline in treating ocular surface diseases may be due to its ability to suppress JNK and ERK signalling activation and inflammatory mediator production in the limbal epithelium...
  18. ncbi request reprint Corticosteroid and doxycycline suppress MMP-9 and inflammatory cytokine expression, MAPK activation in the corneal epithelium in experimental dry eye
    Cintia S de Paiva
    Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine, 6565 Fannin Street, NC 205, Houston, TX 77030, USA
    Exp Eye Res 83:526-35. 2006
    ..Both corticosteroid and doxycycline suppressed this molecular stress response...
  19. ncbi request reprint Neurturin-deficient mice develop dry eye and keratoconjunctivitis sicca
    Xiu Jun Song
    Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA
    Invest Ophthalmol Vis Sci 44:4223-9. 2003
    ..Neurturin-deficient (NRTN(-/-)) mice have defective parasympathetic innervation of their lacrimal glands. This study was conducted to evaluate tear function and ocular surface phenotype in NRTN(-/-) mice...
  20. pmc Age-related T-cell cytokine profile parallels corneal disease severity in Sjogren's syndrome-like keratoconjunctivitis sicca in CD25KO mice
    Cintia S de Paiva
    Ocular Surface Center, Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine, Houston, TX 77030, USA
    Rheumatology (Oxford) 49:246-58. 2010
    ..The objective of this study was to evaluate the severity of corneal epithelial disease and T-cell cytokine profile in the ocular surface tissues of CD25KO mice...
  21. ncbi request reprint Hyperosmolarity-induced apoptosis in human corneal epithelial cells is mediated by cytochrome c and MAPK pathways
    Lihui Luo
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA
    Cornea 26:452-60. 2007
    ..To study whether hyperosmolarity induces apoptosis in human corneal epithelial cells through cytochrome c-mediated death pathways and by activation of mitogen-activated protein kinases (MAPKs)...
  22. ncbi request reprint Desiccating stress stimulates expression of matrix metalloproteinases by the corneal epithelium
    Rosa M Corrales
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA
    Invest Ophthalmol Vis Sci 47:3293-302. 2006
    ..To determine the effects of experimental dryness on production of matrix metalloproteinases (MMPs) and their physiological inhibitors (TIMPs) by the corneal epithelium...
  23. pmc Production and activity of matrix metalloproteinase-9 on the ocular surface increase in dysfunctional tear syndrome
    Suksri Chotikavanich
    Department of Ophthalmology, Ocular Surface Center, Cullen Eye Institute, Baylor College of Medicine, Houston, Texas 77030, USA
    Invest Ophthalmol Vis Sci 50:3203-9. 2009
    ..To evaluate production and activity of metalloproteinase (MMP)-9 on the ocular surface of patients with dysfunctional tear syndrome (DTS) and determine any correlation between MMP-9 activity and clinical parameters...
  24. ncbi request reprint Strain-related cytokine profiles on the murine ocular surface in response to desiccating stress
    Rosa M Corrales
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA
    Cornea 26:579-84. 2007
    ..To evaluate the effects of desiccating ocular surface stress on levels of inflammatory cytokines in the corneal epithelium, conjunctiva, and tear fluid of BALB/c and C57BL/6 mice...
  25. pmc Cell size correlates with phenotype and proliferative capacity in human corneal epithelial cells
    Cintia S de Paiva
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, 6565 Fannin Street, NC 205, Houston, Texas 77030, USA
    Stem Cells 24:368-75. 2006
    ..The smallest cells in population A seem to be enriched for putative stem cells, and small cell size may represent one of the important properties of adult corneal epithelial stem cells...
  26. ncbi request reprint Transglutaminase participates in UVB-induced cell death pathways in human corneal epithelial cells
    Louis Tong
    Ocular Surface Center, Cullen Eye Institute, Baylor College of Medicine, Houston, Texas 77030, USA
    Invest Ophthalmol Vis Sci 47:4295-301. 2006
    ..This study evaluates the role of transglutaminase in regulating tumor necrosis factor (TNF) receptor clustering as well as caspase activation in UVB-induced apoptosis in human corneal epithelial cells...
  27. ncbi request reprint Hyperosmolar saline is a proinflammatory stress on the mouse ocular surface
    Lihui Luo
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA
    Eye Contact Lens 31:186-93. 2005
    ....
  28. pmc Pharmacological cholinergic blockade stimulates inflammatory cytokine production and lymphocytic infiltration in the mouse lacrimal gland
    John D Pitcher
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas 77030, USA
    Invest Ophthalmol Vis Sci 52:3221-7. 2011
    ..To investigate the effects of cholinergic blockade on inflammatory cell infiltration and cytokine production in the mouse lacrimal gland (LG)...
  29. pmc TSLP and downstream molecules in experimental mouse allergic conjunctivitis
    Xiaofen Zheng
    Department of Ophthalmology, Baylor College of Medicine, Ocular Surface Center, Cullen Eye Institute, Houston, Texas 77030, USA
    Invest Ophthalmol Vis Sci 51:3076-82. 2010
    ..To explore the potential role of thymic stromal lymphopoietin (TSLP) and its downstream molecules in the development of ocular allergic inflammation using a short ragweed (SRW)-induced mouse model of allergic conjunctivitis (AC)...
  30. ncbi request reprint Experimental dry eye stimulates production of inflammatory cytokines and MMP-9 and activates MAPK signaling pathways on the ocular surface
    Lihui Luo
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, 6565 Fannin Street, Houston, TX 77030, USA
    Invest Ophthalmol Vis Sci 45:4293-301. 2004
    ....
  31. ncbi request reprint Expression of Th-1 chemokines and chemokine receptors on the ocular surface of C57BL/6 mice: effects of desiccating stress
    Kyung Chul Yoon
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas 77030, USA
    Invest Ophthalmol Vis Sci 48:2561-9. 2007
    ..To evaluate the effects of desiccating ocular surface stress on the expression of chemokines and their receptors by the corneal epithelium and conjunctiva of C57BL/6 and BALB/c mice...
  32. pmc Nerve growth factor and its receptor TrkA serve as potential markers for human corneal epithelial progenitor cells
    Hong Qi
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, 6565 Fannin Street, NC 205, Houston, TX 77030, USA
    Exp Eye Res 86:34-40. 2008
    ..The spatial expression of NGF and TrkA by small clusters of basal cells interspersed between negative cell patches suggests that they are potential markers for human corneal epithelial progenitor cells...
  33. doi request reprint Transcription factor TCF4 maintains the properties of human corneal epithelial stem cells
    Rong Lu
    Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat Sen University, Guangzhou, People s Republic of China
    Stem Cells 30:753-61. 2012
    ..These findings demonstrate that transcription factor TCF4 plays an important role in determining or maintaining the phenotype and functional properties of human corneal epithelial stem cells...
  34. pmc Interferon-γ exacerbates dry eye-induced apoptosis in conjunctiva through dual apoptotic pathways
    Xiaobo Zhang
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas 77030, USA
    Invest Ophthalmol Vis Sci 52:6279-85. 2011
    ..To investigate the role of interferon (IFN)-γ in dry eye-associated conjunctival apoptosis...
  35. pmc Molecular signatures and biological pathway profiles of human corneal epithelial progenitor cells
    Fang Bian
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, TX, USA
    Int J Biochem Cell Biol 42:1142-53. 2010
    ..These genes and related pathways are interesting targets for further identification and isolation of limbal stem cells as well as other tissue-specific adult stem cells...
  36. pmc Chemokine receptors CCR6 and CXCR3 are necessary for CD4(+) T cell mediated ocular surface disease in experimental dry eye disease
    Terry G Coursey
    Ocular Surface Center, Dept of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine, Houston, Texas, United States of America
    PLoS ONE 8:e78508. 2013
    ....
  37. pmc Dendritic cell-derived thrombospondin-1 is critical for the generation of the ocular surface Th17 response to desiccating stress
    Niral B Gandhi
    1 Baylor College of Medicine, 6565 Fannin St, NC 505, Houston, TX 77030, USA
    J Leukoc Biol 94:1293-301. 2013
    ..Reconstitution of TSP-1KO mice with WT DCs prior to DS reversed the resistance of the TSP-1KO to DS-induced immunopathology. In conclusion, DC-derived TSP-1 is critical for generating the Th17 ocular surface response to DS. ..
  38. pmc Desiccating environmental stress exacerbates autoimmune lacrimal keratoconjunctivitis in non-obese diabetic mice
    Kyung Chul Yoon
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA
    J Autoimmun 30:212-21. 2008
    ..These findings indicate that desiccating environmental stress aggravates Sjögren's syndrome-like lacrimal keratoconjunctivitis in the NOD mouse which has defective immunoregulation...
  39. pmc Spontaneous autoimmune dacryoadenitis in aged CD25KO mice
    Ehsan Rahimy
    Ocular Surface Center, Cullen Eye Institute, Baylor College of Medicine, Houston, TX 77030, USA
    Am J Pathol 177:744-53. 2010
    ..Taken together, autoimmune LG infiltration with loss of LG function was observed in CD25KO mice as early as 8 weeks of age. Time-related switch from Th-17 to Th-1 inflammation was noted in CD25KO mice...
  40. pmc Expression and regulation of cornified envelope proteins in human corneal epithelium
    Louis Tong
    Ocular Surface Center, Cullen Eye Institute, Baylor College of Medicine, Houston, Texas 77030, USA
    Invest Ophthalmol Vis Sci 47:1938-46. 2006
    ....
  41. pmc Desiccating stress induces CD4+ T-cell-mediated Sjögren's syndrome-like corneal epithelial apoptosis via activation of the extrinsic apoptotic pathway by interferon-γ
    Xiaobo Zhang
    Ocular Surface Center, Cullen Eye Institute, and the Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA
    Am J Pathol 179:1807-14. 2011
    ..CD4(+) T-cell-produced IFN-γ plays a pivotal role in DS-induced corneal epithelial apoptosis via activation of the extrinsic apoptotic pathway...
  42. pmc Suppressive effects of azithromycin on zymosan-induced production of proinflammatory mediators by human corneal epithelial cells
    De Quan Li
    Department of Ophthalmology, Baylor College of Medicine, Ocular Surface Center, Cullen Eye Institute, Houston, Texas 77030, USA
    Invest Ophthalmol Vis Sci 51:5623-9. 2010
    ..This study was conducted to explore the suppressive effects of azithromycin on the production of proinflammatory mediators by human corneal epithelial cells (HCECs) stimulated by a fungal component, zymosan...
  43. pmc Induction of Th17 differentiation by corneal epithelial-derived cytokines
    Xiaofen Zheng
    Department of Ophthalmology, Ocular Surface Center, Cullen Eye Institute, Baylor College of Medicine, Houston, Texas 77030, USA
    J Cell Physiol 222:95-102. 2010
    ..These findings demonstrate for the first time that Th17 differentiation can be promoted by cytokines produced by corneal epithelium that are exposed to hyperosmotic, microbial, and inflammatory stimuli...
  44. pmc Altered morphology and function of the lacrimal functional unit in protein kinase C{alpha} knockout mice
    Zhuo Chen
    Department of Ophthalmology, The Ocular Surface Center, Cullen Eye Institute, Baylor College of Medicine, Houston, Texas 77030, USA
    Invest Ophthalmol Vis Sci 51:5592-600. 2010
    ..In this study, the lacrimal function unit (LFU) and the stressed wound-healing response were examined in PKCα(-/-) mice...
  45. ncbi request reprint Topical cyclosporine inhibits conjunctival epithelial apoptosis in experimental murine keratoconjunctivitis sicca
    Bradley Strong
    Ocular Surface Center, Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine, Houston, Texas 77030, USA
    Cornea 24:80-5. 2005
    ..The purpose of this study is to determine if topical CsA treatment inhibits conjunctival epithelial apoptosis in a murine model of KCS...
  46. doi request reprint Glial cell-derived neurotrophic factor gene delivery enhances survival of human corneal epithelium in culture and the overexpression of GDNF in bioengineered constructs
    Hong Qi
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA
    Exp Eye Res 87:580-6. 2008
    ....
  47. pmc Phenotypic characterization of human corneal epithelial cells expanded ex vivo from limbal explant and single cell cultures
    Hyun Seung Kim
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, 6565 Fannin Street, NC 205, Houston, TX 77030, USA
    Exp Eye Res 79:41-9. 2004
    ..Slow-cycling BrdU-label retaining cells, that are characteristic of stem cells, were identified in the cultures...
  48. pmc A native-like corneal construct using donor corneal stroma for tissue engineering
    Jing Lin
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas, United States of America
    PLoS ONE 7:e49571. 2012
    ..This construct may have great potential for clinical use in corneal reconstruction...
  49. pmc Evaluation of the transforming growth factor-beta activity in normal and dry eye human tears by CCL-185 cell bioassay
    Xiaofen Zheng
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA
    Cornea 29:1048-54. 2010
    ..To develop a new bioassay method using human lung epithelial cells (CCL-185) to assess activity of transforming growth factor beta (TGF-beta) in human tear fluid from normal subjects and patients with dry eye...
  50. pmc Tear cytokine profiles in dysfunctional tear syndrome
    Helene Lam
    Department of Ophthalmology, Ocular Surface Center, Cullen Eye Institute, Baylor College of Medicine, Houston, Texas, USA
    Am J Ophthalmol 147:198-205. e1. 2009
    ..To compare tear cytokine and chemokine concentrations in asymptomatic control and Dysfunctional Tear syndrome (DTS) patients and determine the correlations between tear inflammatory mediators and clinical severity...
  51. pmc Association between high tear epidermal growth factor levels and corneal subepithelial fibrosis in dry eye conditions
    Kavita Rao
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas 77030, USA
    Invest Ophthalmol Vis Sci 51:844-9. 2010
    ..To compare tear epidermal growth factor (EGF) concentration in dry eye (DE) conditions and determine correlations between EGF levels and severity of symptoms and ocular surface signs...
  52. pmc A potential link between bacterial pathogens and allergic conjunctivitis by dendritic cells
    Ruzhi Deng
    School of Optometry and Ophthalmology, Wenzhou Medical College, Wenzhou, China Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, TX, USA
    Exp Eye Res 120:118-26. 2014
    ..Our findings demonstrate that DCs not only respond to TSLP, but also produce TSLP via TLR/MyD88/NFκB pathways in response to bacterial pathogens, suggesting a potential link between bacteria and allergic disease. ..
  53. pmc Topical interferon-gamma neutralization prevents conjunctival goblet cell loss in experimental murine dry eye
    Xiaobo Zhang
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, 6565 Fannin Street, NC505, Houston, TX 77030, USA Shenyang He Eye Hospital, Shenyang, Liaoning, China
    Exp Eye Res 118:117-24. 2014
    ..In summary, this study demonstrated that topical neutralization of IFN-γ prevents GC loss via modulating apoptosis and maintaining IL-13 signaling. ..
  54. ncbi request reprint Doxycycline inhibits TGF-beta1-induced MMP-9 via Smad and MAPK pathways in human corneal epithelial cells
    Hyun Seung Kim
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas, USA
    Invest Ophthalmol Vis Sci 46:840-8. 2005
    ....
  55. ncbi request reprint Potential localization of putative stem/progenitor cells in human bulbar conjunctival epithelium
    Hong Qi
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas
    J Cell Physiol 225:180-5. 2010
    ....
  56. pmc Desiccating stress decreases apical corneal epithelial cell size--modulation by the metalloproteinase inhibitor doxycycline
    Robert M Beardsley
    Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine, Houston, TX, USA
    Cornea 27:935-40. 2008
    ..We investigated the effects of desiccating stress on murine corneal apical epithelial cell area and desquamation by using 4 defined parameters and evaluated the effects of the metalloproteinase inhibitor doxycycline on this process...
  57. pmc An immunoprotective privilege of corneal epithelial stem cells against Th17 inflammatory stress by producing glial cell-derived neurotrophic factor
    Fang Bian
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas 77030, USA
    Stem Cells 28:2172-81. 2010
    ..This may represent a unique immunoprotective property of limbal stem cells against inflammatory challenges on the ocular surface...
  58. ncbi request reprint Effects of contact lens multipurpose solutions on human corneal epithelial survival and barrier function
    Eliseu Y Chuang
    Department of Ophthalmology, Ocular Surface Center, Cullen Eye Institute, Baylor College of Medicine, Houston, TX 77030, USA
    Eye Contact Lens 34:281-6. 2008
    ..To examine the effect of 4 commercially available contact lens multipurpose solutions (MPS) on the viability and barrier function of human corneal epithelial cells in vitro...
  59. doi request reprint Human corneal epithelium-derived thymic stromal lymphopoietin links the innate and adaptive immune responses via TLRs and Th2 cytokines
    Ping Ma
    Department of Ophthalmology, Ocular Surface Center, Cullen Eye Institute, Baylor College of Medicine, Houston, Texas 77030, USA
    Invest Ophthalmol Vis Sci 50:2702-9. 2009
    ..To explore the crucial role of the human corneal epithelium-derived proallergic cytokine thymic stromal lymphopoietin (TSLP) in initiation and regulation of immune responses...
  60. pmc ABCG2 transporter identifies a population of clonogenic human limbal epithelial cells
    Cintia S de Paiva
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, 6565 Fannin St, NC 205, Houston, Texas 77030, USA
    Stem Cells 23:63-73. 2005
    ....
  61. pmc Patterned expression of neurotrophic factors and receptors in human limbal and corneal regions
    Hong Qi
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA
    Mol Vis 13:1934-41. 2007
    ..To evaluate the expression patterns of neurotrophic factors (NTFs) and their receptors in the human cornea with the intention of exploring the role of NTFs in maintaining corneal epithelial stem cells in the limbus...
  62. doi request reprint The impact of the Boston ocular surface prosthesis on wavefront higher-order aberrations
    Koray Gumus
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas 77030, USA
    Am J Ophthalmol 151:682-690.e2. 2011
    ....
  63. pmc The β-catenin/Tcf4/survivin signaling maintains a less differentiated phenotype and high proliferative capacity of human corneal epithelial progenitor cells
    Rong Lu
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, TX, USA
    Int J Biochem Cell Biol 43:751-9. 2011
    ..This study may have high impact and clinic implication on the expansion of corneal epithelial stem cells in regenerative medicine, especially for ocular surface reconstruction...
  64. doi request reprint Effects of osmoprotectants on hyperosmolar stress in cultured human corneal epithelial cells
    Rosa M Corrales
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA
    Cornea 27:574-9. 2008
    ..This study assessed the effect of osmoprotectant compounds on the activation state of mitogen-activated protein (MAP) kinases in human corneal epithelial cells incubated in hyperosmolar conditions...
  65. pmc Gap junction protein connexin 43 serves as a negative marker for a stem cell-containing population of human limbal epithelial cells
    Zhuo Chen
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas 77030, USA
    Stem Cells 24:1265-73. 2006
    ..Our findings suggest that human limbal basal cells do not express connexin 43, which could serve as a negative cell surface marker for the stem cell-containing population of human limbal epithelial cells...
  66. pmc Factors predicting the ocular surface response to desiccating environmental stress
    Anastasia Alex
    George R Brown School of Engineering, Rice University, Houston, TX, USA
    Invest Ophthalmol Vis Sci 54:3325-32. 2013
    ..To identify factors predicting the ocular surface response to experimental desiccating stress...
  67. pmc Targeted inhibition of p57 and p15 blocks transforming growth factor beta-inhibited proliferation of primary cultured human limbal epithelial cells
    Zhuo Chen
    The Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA
    Mol Vis 12:983-94. 2006
    ..To evaluate the role of cyclin-dependent kinase inhibitors p57 and p15 in transforming growth factor (TGF)-beta1 or TGF-beta2 inhibited proliferation of primary cultured human limbal epithelial cells using short interfering RNA (siRNA)...
  68. pmc Noninvasive assessment of tear stability with the tear stability analysis system in tear dysfunction patients
    Koray Gumus
    Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas 77030, USA
    Invest Ophthalmol Vis Sci 52:456-61. 2011
    ..To evaluate tear film stability in patients with tear dysfunction and an asymptomatic control group by using the novel, noninvasive Tear Stability Analysis System (TSAS)...
  69. ncbi request reprint Assessing the severity of keratitis sicca with videokeratoscopic indices
    Cintia Sade de Paiva
    Ocular Surface Center, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas, USA
    Ophthalmology 110:1102-9. 2003
    ..To determine the correlation between the regularity indices of the Tomey TMS-2N computerized videokeratoscopy (CVK) instrument (Tomey, Waltham, MA) with conventional measures of dry eye symptoms and disease...

Research Grants11

  1. PATHOGENESIS OF CONJUNCTIVAL SQUAMOUS METAPLASIA
    STEPHEN PFLUGFELDER; Fiscal Year: 2000
    ....
  2. PATHOGENESIS OF CONJUNCTIVAL SQUAMOUS METAPLASIA
    STEPHEN CARL PFLUGFELDER; Fiscal Year: 2010
    ....
  3. PATHOGENESIS OF CONJUNCTIVAL SQUAMOUS METAPLASIA
    STEPHEN PFLUGFELDER; Fiscal Year: 2009
    ....
  4. Environmentally-Induced Ocular Surface Inflammation Mimicking Sjogren's Syndrome
    STEPHEN PFLUGFELDER; Fiscal Year: 2009
    ..We further propose that the severity of this ALKC is worsened by defective self tolerance mechanisms. ..
  5. PATHOGENESIS OF CONJUNCTIVAL SQUAMOUS METAPLASIA
    STEPHEN PFLUGFELDER; Fiscal Year: 2007
    ....
  6. Pathogenesis of Conjunctival Squamous
    STEPHEN PFLUGFELDER; Fiscal Year: 2005
    ....
  7. Pathogenesis of Conjunctival Squamous
    STEPHEN PFLUGFELDER; Fiscal Year: 2004
    ....
  8. Pathogenesis of Conjunctival Squamous
    STEPHEN PFLUGFELDER; Fiscal Year: 2003
    ....
  9. Pathogenesis of Conjunctival Squamous
    STEPHEN PFLUGFELDER; Fiscal Year: 2002
    ....
  10. Environmentally-Induced Ocular Surface Inflammation Mimicking Sjogren's Syndrome
    Michael J Stern; Fiscal Year: 2010
    ..We further propose that the severity of this ALKC is worsened by defective self tolerance mechanisms. ..