Yasuhiro Nishiyama

Summary

Affiliation: Texas Medical Center
Country: USA

Publications

  1. ncbi request reprint An efficient synthesis of N-tert-butoxycarbonyl-O-cyclohexyl-L-tyrosine
    Y Nishiyama
    Department of Industrial Chemistry, Faculty of Engineering, Seikei University, Tokyo, Japan
    Chem Pharm Bull (Tokyo) 49:233-5. 2001
  2. ncbi request reprint Towards covalent vaccination: improved polyclonal HIV neutralizing antibody response induced by an electrophilic gp120 V3 peptide analog
    Yasuhiro Nishiyama
    Chemical Immunology Research Center, Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, Houston, Texas 77030, USA
    J Biol Chem 282:31250-6. 2007
  3. ncbi request reprint Anti-HIV-1 peptides derived from partial amino acid sequences of CC-chemokine RANTES. Regulated upon activation, normal T-cell expressed and secreted
    Yasuhiro Nishiyama
    Chemical Immunology and Therapeutics Research Center, Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, 77030, USA
    Bioorg Med Chem 10:4113-7. 2002
  4. ncbi request reprint Covalent reactivity of phosphonate monophenyl esters with serine proteinases: an overlooked feature of presumed transition state analogs
    Yasuhiro Nishiyama
    Chemical Immunology and Therapeutics Research Center, Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
    Arch Biochem Biophys 402:281-8. 2002
  5. ncbi request reprint Toward selective covalent inactivation of pathogenic antibodies: a phosphate diester analog of vasoactive intestinal peptide that inactivates catalytic autoantibodies
    Yasuhiro Nishiyama
    Chemical Immunology and Therapeutics Research Center, Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, Houston, Texas 77030, USA
    J Biol Chem 279:7877-83. 2004
  6. ncbi request reprint VIPase autoantibodies in Fas-defective mice and patients with autoimmune disease
    Yogesh Bangale
    Chemical Immunology Research Center, Department of Pathology, University of Texas Medical School, 6431 Fannin, Houston, TX 77030, USA
    FASEB J 17:628-35. 2003
  7. pmc Antibodies to a superantigenic glycoprotein 120 epitope as the basis for developing an HIV vaccine
    Stephanie A Planque
    Department of Pathology and Laboratory Medicine, Chemical Immunology Research Center, University of Texas Medical School at Houston, Houston, TX 77030, USA
    J Immunol 189:5367-81. 2012
  8. ncbi request reprint Characterization of gp120 hydrolysis by IgA antibodies from humans without HIV infection
    Stephanie Planque
    Chemical Immunology Research Center, Department of Pathology and Laboratory Medicine and Hemophilia and Thrombophilia Center, University of Texas Houston Medical School, Houston, Texas 77030, USA
    AIDS Res Hum Retroviruses 23:1541-54. 2007
  9. pmc Exceptional amyloid beta peptide hydrolyzing activity of nonphysiological immunoglobulin variable domain scaffolds
    Hiroaki Taguchi
    Chemical Immunology Research Center, Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, Houston, Texas 77030, USA
    J Biol Chem 283:36724-33. 2008
  10. pmc Catalytic antibodies to amyloid beta peptide in defense against Alzheimer disease
    Hiroaki Taguchi
    Chemical Immunology Research Center, University of Texas Houston Medical School, Houston, TX 77030, USA
    Autoimmun Rev 7:391-7. 2008

Research Grants

  1. Neutralizing antibodies raised by covalent immunization
    Yasuhiro Nishiyama; Fiscal Year: 2007

Collaborators

Detail Information

Publications44

  1. ncbi request reprint An efficient synthesis of N-tert-butoxycarbonyl-O-cyclohexyl-L-tyrosine
    Y Nishiyama
    Department of Industrial Chemistry, Faculty of Engineering, Seikei University, Tokyo, Japan
    Chem Pharm Bull (Tokyo) 49:233-5. 2001
    ..The new synthetic method described here is convenient for practical use, and would facilitate the widespread use of the Chx group for the hydroxy-protection of Tyr...
  2. ncbi request reprint Towards covalent vaccination: improved polyclonal HIV neutralizing antibody response induced by an electrophilic gp120 V3 peptide analog
    Yasuhiro Nishiyama
    Chemical Immunology Research Center, Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, Houston, Texas 77030, USA
    J Biol Chem 282:31250-6. 2007
    ..These results suggest that electrophilic immunization induces a sufficiently robust nucleophilic Ab response to enhance the anti-microbial efficacy of candidate polypeptide vaccines...
  3. ncbi request reprint Anti-HIV-1 peptides derived from partial amino acid sequences of CC-chemokine RANTES. Regulated upon activation, normal T-cell expressed and secreted
    Yasuhiro Nishiyama
    Chemical Immunology and Therapeutics Research Center, Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, 77030, USA
    Bioorg Med Chem 10:4113-7. 2002
    ..The results indicate that multiple regions, including the N-terminal part responsible for chemotactic activity, are involved in anti-HIV-1 activity of RANTES, yielding possible lead compounds for anti-HIV-1 agents...
  4. ncbi request reprint Covalent reactivity of phosphonate monophenyl esters with serine proteinases: an overlooked feature of presumed transition state analogs
    Yasuhiro Nishiyama
    Chemical Immunology and Therapeutics Research Center, Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
    Arch Biochem Biophys 402:281-8. 2002
    ..47 vs 2.0 min(-1)). These observations suggest that the covalent reactivity of phosphonate monoesters contributes to their interactions with serine proteinases, including certain proteolytic antibodies...
  5. ncbi request reprint Toward selective covalent inactivation of pathogenic antibodies: a phosphate diester analog of vasoactive intestinal peptide that inactivates catalytic autoantibodies
    Yasuhiro Nishiyama
    Chemical Immunology and Therapeutics Research Center, Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, Houston, Texas 77030, USA
    J Biol Chem 279:7877-83. 2004
    ..These results suggest a novel route to antigen-specific covalent targeting of pathogenic Abs...
  6. ncbi request reprint VIPase autoantibodies in Fas-defective mice and patients with autoimmune disease
    Yogesh Bangale
    Chemical Immunology Research Center, Department of Pathology, University of Texas Medical School, 6431 Fannin, Houston, TX 77030, USA
    FASEB J 17:628-35. 2003
    ..These observations show that VIP is a target for specific catalytic autoantibodies in autoimmune disease...
  7. pmc Antibodies to a superantigenic glycoprotein 120 epitope as the basis for developing an HIV vaccine
    Stephanie A Planque
    Department of Pathology and Laboratory Medicine, Chemical Immunology Research Center, University of Texas Medical School at Houston, Houston, TX 77030, USA
    J Immunol 189:5367-81. 2012
    ..The studies indicate broad and potent HIV neutralization by constitutive Abs as an innate, germline-encoded activity directed to the superantigenic CD4BD(core) epitope that is available for amplification for vaccination against HIV...
  8. ncbi request reprint Characterization of gp120 hydrolysis by IgA antibodies from humans without HIV infection
    Stephanie Planque
    Chemical Immunology Research Center, Department of Pathology and Laboratory Medicine and Hemophilia and Thrombophilia Center, University of Texas Houston Medical School, Houston, Texas 77030, USA
    AIDS Res Hum Retroviruses 23:1541-54. 2007
    ..The neutralizing activity was specifically inhibited by an electrophilic inhibitor of the catalytic activity. The existence of catalytic IgAs to gp120 in uninfected humans suggests their role in resistance to HIV...
  9. pmc Exceptional amyloid beta peptide hydrolyzing activity of nonphysiological immunoglobulin variable domain scaffolds
    Hiroaki Taguchi
    Chemical Immunology Research Center, Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, Houston, Texas 77030, USA
    J Biol Chem 283:36724-33. 2008
    ..In view of their superior catalytic activity, the VL domain IgVs may help attain clearance of medically important antigens more efficiently than natural Igs...
  10. pmc Catalytic antibodies to amyloid beta peptide in defense against Alzheimer disease
    Hiroaki Taguchi
    Chemical Immunology Research Center, University of Texas Houston Medical School, Houston, TX 77030, USA
    Autoimmun Rev 7:391-7. 2008
    ..Reduced Abeta aggregation and neurotoxicity attributable to the catalytic function were evident. These findings provide a foundation for development of catalytic Igs for AD immunotherapy...
  11. ncbi request reprint Towards irreversible HIV inactivation: stable gp120 binding by nucleophilic antibodies
    Yasuhiro Nishiyama
    Chemical Immunology and Therapeutics Research Center, Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, Houston, Texas 77030, USA
    J Mol Recognit 19:423-31. 2006
    ..These findings suggest the feasibility of raising antibodies capable of long-lasting inactivation of antigens by electrophilic immunization...
  12. ncbi request reprint Naturally occurring proteolytic antibodies: selective immunoglobulin M-catalyzed hydrolysis of HIV gp120
    Sudhir Paul
    Chemical Immunology and Therapeutics Research Center, Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, Houston, Texas 77030, USA
    J Biol Chem 279:39611-9. 2004
    ..These observations suggest that IgMs can selectively cleave gp120 by a nucleophilic mechanism and raise the possibility of their role as defense enzymes...
  13. pmc Toward effective HIV vaccination: induction of binary epitope reactive antibodies with broad HIV neutralizing activity
    Yasuhiro Nishiyama
    Department of Pathology and Laboratory Medicine, Chemical Immunology Research Center, University of Texas Houston Medical School, Houston, Texas 77030, USA
    J Biol Chem 284:30627-42. 2009
    ..We present models accounting for improved CD4-binding site recognition and broad HIV neutralizing activity of the mAbs, long sought goals in HIV vaccine development...
  14. pmc Neutralization of genetically diverse HIV-1 strains by IgA antibodies to the gp120-CD4-binding site from long-term survivors of HIV infection
    Stephanie Planque
    Chemical Immunology Research Center and Gulf States Hemophilia and Thrombophilia Center, Departments of Pathology and Pediatrics, University of Texas Houston Medical School, Houston, Texas, USA
    AIDS 24:875-84. 2010
    ..To identify an HIV epitope suitable for vaccine development...
  15. ncbi request reprint Autoantibody-catalyzed hydrolysis of amyloid beta peptide
    Hiroaki Taguchi
    Chemical Immunology Research Center, Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, Houston, Texas 77030, USA
    J Biol Chem 283:4714-22. 2008
    ..Our results suggest that catalytic IgM autoantibodies can help clear Abeta, and they open the possibility of using catalytic Abs for AD immunotherapy...
  16. ncbi request reprint Cross-clade HIV-1 neutralization by an antibody fragment from a lupus phage display library
    Sangeeta Karle
    Chemical Immunology Research Center, Department of Pathology, University of Texas, Houston Medical School, USA
    AIDS 18:329-31. 2004
    ..The scFv neutralized R5 and X4-dependent HIV-1 strains from clades B, C, and D. The lupus repertoire may be useful as a source of neutralizing antibodies to HIV...
  17. pmc Back to the future: covalent epitope-based HIV vaccine development
    Sudhir Paul
    Department of Pathology and Laboratory Medicine, Chemical Immunology Research Center, University of Texas Houston Medical School, 6431 Fannin, MSB 2 230A, Houston, TX 77030, USA
    Expert Rev Vaccines 9:1027-43. 2010
    ..We summarize evidence supporting development of covalent immunization as the first vaccine strategy with the potential to induce an antibody response to a conserved HIV epitope that neutralizes genetically divergent HIV strains...
  18. ncbi request reprint Naturally occurring catalytic antibodies: evidence for preferred development of the catalytic function in IgA class antibodies
    Yukie Mitsuda
    Department of Pathology and Laboratory Medicine, Chemical Immunology Research Center, University of Texas Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
    Mol Biotechnol 36:113-22. 2007
    ..These observations suggest that mechanisms underlying B cell clonal selection favor the retention and improvement of catalytic activity in the IgA, but not the IgG compartment of the immune response...
  19. ncbi request reprint Broadly distributed chemical reactivity of natural antibodies expressed in coordination with specific antigen binding activity
    Stephanie Planque
    Chemical Immunology and Therapeutics Research Center, Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, 77030, USA
    J Biol Chem 278:20436-43. 2003
    ....
  20. ncbi request reprint Specific HIV gp120-cleaving antibodies induced by covalently reactive analog of gp120
    Sudhir Paul
    Chemical Immunology Research Center, Department of Pathology, University of Texas, Houston Medical School, 77030, USA
    J Biol Chem 278:20429-35. 2003
    ..These findings suggest the feasibility of raising antigen-specific proteolytic antibodies on demand by covalent immunization...
  21. ncbi request reprint A mechanism-based probe for gp120-Hydrolyzing antibodies
    Hiroaki Taguchi
    Chemical Immunology and Therapeutics Research Center, Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
    Bioorg Med Chem Lett 12:3167-70. 2002
    ..These results reveal that the peptidyl phosphonate ester can serve as a probe for the antigen recognition and catalytic subsites of proteolytic antibodies...
  22. ncbi request reprint Antibodies to the superantigenic site of HIV-1 gp120: hydrolytic and binding activities of the light chain subunit
    Yasuhiro Nishiyama
    Chemical Immunology and Therapeutics Research Center, Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
    Mol Immunol 44:2707-18. 2007
    ..The pairing of such light chains with heavy chains capable of gp120(SAg) recognition represents a potential mechanism for generating protective Abs with enhanced HIV binding strength and anti-viral proteolytic activity...
  23. ncbi request reprint Ontogeny of proteolytic immunity: IgM serine proteases
    Stephanie Planque
    Department of Pathology and Laboratory Medicine, University of Texas, Houston Medical School, Houston, Texas 77030, USA
    J Biol Chem 279:14024-32. 2004
    ..These observations indicate the existence of serine protease-like BCRs and secreted IgM Abs as innate immunity components with potential roles in B cell development and Ab effector functions...
  24. pmc Immunological origin and functional properties of catalytic autoantibodies to amyloid beta peptide
    Sudhir Paul
    Department of Pathology and Laboratory Medicine, Chemical Immunology Research Center, University of Texas Houston Medical School, Houston, TX 77030, USA
    J Clin Immunol 30:S43-9. 2010
    ....
  25. pmc Catalytic antibodies to HIV: physiological role and potential clinical utility
    Stephanie Planque
    Department of Pathology, University of Texas Houston Medical School, Houston, TX, USA
    Autoimmun Rev 7:473-9. 2008
    ..Further study of the properties of the naturally occurring anti-SAg catalytic Igs should provide valuable guidance in designing a prophylactic vaccine that amplifies protective catalytic immunity to HIV...
  26. doi request reprint Nature and nurture of catalytic antibodies
    Sudhir Paul
    Chemical Immunology Research Center, Department of Pathology, University of Texas Houston Medical School, Texas, USA
    Adv Exp Med Biol 750:56-75. 2012
    ..These concepts may be useful for developing novel vaccines that capitalize and improve on constitutive antibody functions for protection against microbes...
  27. ncbi request reprint Broadly distributed nucleophilic reactivity of proteins coordinated with specific ligand binding activity
    Yasuhiro Nishiyama
    Chemical Immunology and Therapeutics Research Center, Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
    J Mol Recognit 18:295-306. 2005
    ..These observations suggest the potential of EPs for specific and covalent targeting of proteins, and raise the possibility of nucleophile-electrophile pairing as a novel mechanism stabilizing protein-protein complexes...
  28. ncbi request reprint Antibodies as defensive enzymes
    Sudhir Paul
    Chemical Immunology and Therapeutics Research Center, Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, MSB 2 250, 6431 Fannin, Houston, TX 77030, USA
    Springer Semin Immunopathol 26:485-503. 2005
    ....
  29. ncbi request reprint Autoantibodies to the epidermal growth factor receptor in systemic sclerosis, lupus, and autoimmune mice
    Stephanie Planque
    Chemical Immunology and Therapeutics Research Center, Department of Pathology and Internal Medicine, University of Texas Houston Medical School, Houston, Texas 77030, USA
    FASEB J 17:136-43. 2003
    ..Short-lived but strong inhibition of cellular DNA synthesis was observed in the presence of the autoantibodies. We concluded that autoantibody responses to EGFR hold the potential of fulfilling a pathogenic role in autoimmune disease...
  30. ncbi request reprint Prospects for immunotherapeutic proteolytic antibodies
    Yong Xin Zhou
    Chemical Immunology and Therapeutics Research Center, Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
    J Immunol Methods 269:257-68. 2002
    ..In reference to therapy of HIV infection, assurance is required that the Abs recognize the native conformation of gp120 expressed as a trimer on the virus surface...
  31. ncbi request reprint Vasoactive intestinal peptide binding autoantibodies in autoimmune humans and mice
    Yogesh Bangale
    Department of Pathology and Medicine, Chemical Immunology Research Center, University of Texas Medical School, Houston, TX, USA
    Peptides 23:2251-7. 2002
    ..The depletion of VIP by specific antibodies in autoimmune disease may interfere with VIP regulation of T cells and inflammatory cells and result in further amplification of autoreactive immunological responses...
  32. pmc Constant domain-regulated antibody catalysis
    Gopal Sapparapu
    Chemical Immunology Research Center, Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, Houston, Texas 77030, USA
    J Biol Chem 287:36096-104. 2012
    ....
  33. pmc Covalent inactivation of factor VIII antibodies from hemophilia A patients by an electrophilic FVIII Analog
    Stephanie Planque
    Chemical Immunology Research Center, Department of Pathology, University of Texas Houston Medical School, Houston, Texas 77030, USA
    J Biol Chem 283:11876-86. 2008
    ..E-FVIII is a prototype reagent able to attain irreversible and specific inactivation of pathogenic Abs...
  34. ncbi request reprint Antibody light chain-catalyzed hydrolysis of a hepatitis C virus peptide
    Hiroaki Taguchi
    Chemical Immunology and Therapeutics Research Center, Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, 6431 Fannin, 77030, USA
    Bioorg Med Chem Lett 14:4529-32. 2004
    ..One of the 39 light chains studied hydrolyzed the Val197-Ser198 bond of the peptide with Km and kcat values of 223 +/- 7 microM and 0.087 +/- 0.001 min(-1)...
  35. pmc Antigen-specific proteolysis by hybrid antibodies containing promiscuous proteolytic light chains paired with an antigen-binding heavy chain
    Gopal Sapparapu
    Chemical Immunology Research Center, Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, Houston, Texas 77030, USA
    J Biol Chem 284:24622-33. 2009
    ..These studies provide proof-of-principle that the catalytic activity of a light chain can be rendered antigen-specific by pairing with a noncovalently binding heavy chain subunit...
  36. pmc Beneficial catalytic immunity to abeta peptide
    Sudhir Paul
    Chemical Immunology Research Center, University of Texas Houston Medical School, Houston, Texas 77030, USA
    Rejuvenation Res 13:179-87. 2010
    ..We present our view that catalytic antibodies are likely to emerge as more efficacious and safer immunotherapy reagents compared to traditional Abeta-binding antibodies...
  37. ncbi request reprint Theory of proteolytic antibody occurrence
    Sudhir Paul
    Chemical Immunology and Therapeutics Research Center, Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, 6431 Fannin, 77030, USA
    Immunol Lett 103:8-16. 2006
    ..This article attempts to provide a rational basis for natural Ab catalysis, in the hope that understanding this phenomenon will stimulate medical and basic science advances in the field...
  38. pmc CD4 binding determinant mimicry for HIV vaccine design
    Yasuhiro Nishiyama
    Chemical Immunology Research Center, Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School Houston, TX, USA
    Front Immunol 3:383. 2012
    ..Successful vaccine targeting of the CD4BD will require a sufficiently rigid immunogen that mimics the native epitope conformation and bypasses B cell checkpoints restricting synthesis of the neutralizing antibodies...
  39. ncbi request reprint Phosphonate ester probes for proteolytic antibodies
    S Paul
    Department of Pathology and Laboratory Medicine, Chemical Immunology and Therapeutics Research Center, University of Texas Houston Medical School, Houston, Texas 77030, USA
    J Biol Chem 276:28314-20. 2001
    ..The covalent reactivity of Abs with phosphonate esters suggests their ability to recapitulate the catalytic mechanism utilized by classical serine proteases...
  40. pmc Constitutive production of catalytic antibodies to a Staphylococcus aureus virulence factor and effect of infection
    Eric L Brown
    Center for Infectious Diseases, University of Texas School of Public Health, Houston, Texas 77030, USA
    J Biol Chem 287:9940-51. 2012
    ..These data suggest a potential defense function for constitutively produced catalytic antibodies to a putative superantigenic site of Efb, but an adaptive catalytic response appears to be proscribed...
  41. ncbi request reprint Homeostatic regulation of intestinal villous epithelia by B lymphocytes
    Yasuhiro Nishiyama
    Department of Microbiology, Keio University School of Medicine, Nippon Medical School, Tokyo, Japan
    J Immunol 168:2626-33. 2002
    ....
  42. ncbi request reprint Identification of multiple isolated lymphoid follicles on the antimesenteric wall of the mouse small intestine
    Hiromasa Hamada
    Department of Microbiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
    J Immunol 168:57-64. 2002
    ..These findings indicate that ILF, PP, and CP constitute three distinct organized gut-associated lymphoid tissues that reside in the lamina propria of the mouse small intestine...
  43. ncbi request reprint Catalytic antibodies and their applications
    Carl Veith Hanson
    Viral and Rickettsial Disease Laboratory, California Department of Health Services, Richmond, 94804, USA
    Curr Opin Biotechnol 16:631-6. 2005
    ..Artificial CAbs are likely to find increasing applications in research, clinical medicine, diagnostics and manufacturing...
  44. ncbi request reprint Onset of hepatic erythropoiesis after malarial infection in mice
    Ramesh C Halder
    Department of Immunology, Niigata University School of Medicine, Niigata 951 8510, Japan
    Parasitol Int 52:259-68. 2003
    ..Many clusters of red blood cells appeared de novo in the parenchymal space of the liver. These results revealed that malarial infection had a potential to induce the onset of hepatic erythropoiesis in mice...

Research Grants1

  1. Neutralizing antibodies raised by covalent immunization
    Yasuhiro Nishiyama; Fiscal Year: 2007
    ..The studies are likely to strengthen the foundation for further development of neutralizing antibodies capable of permanent virus inactivation. ..